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Detection of dengue using PAMAM dendrimer integrated tapered optical fiber sensor

Mustapha Kamil Y 1 , Al-Rekabi SH 2 , Yaacob MH 1 , Syahir A 3 , Chee HY 4 , Mahdi MA 1 Show all authors , Abu Bakar MH 5

Affiliations 

  • 1 Wireless and Photonic Networks Research Centre, Faculty of Engineering, Universiti Putra Malaysia, 43400, Serdang, Malaysia
  • 2 Electrical Engineering Department, Al Suwayrah Technical Institute, Middle Technical University in Baghdad, Baghdad, Iraq
  • 3 Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400, Serdang, Malaysia
  • 4 Department of Microbiology and Parasitology, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Serdang, 43400, Serdang, Malaysia
  • 5 Wireless and Photonic Networks Research Centre, Faculty of Engineering, Universiti Putra Malaysia, 43400, Serdang, Malaysia. mhab@ieee.org
Sci Rep, 2019 09 17;9(1):13483.
PMID: 31530893 DOI: 10.1038/s41598-019-49891-7

Abstract

The exponential escalation of dengue cases has indeed become a global health crisis. This work elaborates on the development of a biofunctionalized tapered optical fiber (TOF) based sensor with the integration of polyamidoamine (PAMAM) dendrimer for the detection of dengue E protein. The dimension of the TOF generated an evanescent field that was sensitive to any changes in the external medium while the integration of PAMAM promoted more adhesion of bio-recognition molecules; anti-DENV II E protein antibodies; that were complementary to the targeted protein. This in return created more active sites for the absorption of DENV II E proteins onto the tapered region. The resolution and detection limit of the sensor are 19.53 nm/nM and 1 pM, respectively with Kd = 1.02 × 10-10 M.

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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