Radon exists naturally in the air. It can accumulate inside the buildings which may affect the indoor air quality. Radon is a radioactive gas that produces alpha particles during decay time. The alpha particles might cause harm to the human lungs and stomach. Inhalation of radon is one of the causes of lung cancer disease. Samples of inhaled radon in different rooms at the workplace were taken hourly through a passive diffusion chamber. The detection method was done using Alpha Spectrometry. The short term measurement was applied in the study to monitor the average weekly radon reading in different rooms in the Medical Imaging Laboratory of the University of Selangor (UNISEL). All tested rooms showed the existence of radon gas with different concentrations. Some of them showed the maximum reading of radon concentration which was higher than the radon action level of 148Bq/m3 or 4pCi/l. Their weekly average of radon concentration is contributing almost 50% of the accumulated radon concentration in the laboratory. It is highly recommended that monitoring the concentration of radon in indoor air is performed to ensure it is at a safe and healthy level.