Curcumin-loaded niosomes downregulate mRNA expression of pro-inflammatory markers involved in asthma: an in vitro study

Jin-Ying Wong 1 , Yin Ng Z 1 , Mehta M 2 , Shukla SD 3 , Panneerselvam J 4 , Madheswaran T 4 Show all authors , Gupta G 5 , Negi P 6 , Kumar P 7 , Pillay V 7 , Hsu A 3 , Hansbro NG 8 , Wark P 3 , Bebawy M 2 , Hansbro PM 8 , Dua K 2 , Chellappan DK 9

Affiliations 

  • 1 School of Pharmacy, International Medical University, Bukit Jalil, Kuala Lumpur 57000, Malaysia
  • 2 Discipline of Pharmacy, Graduate School of Health, University of Technology Sydney, NSW 2007, Australia
  • 3 Priority Research Centre for Healthy Lungs, University of Newcastle & Hunter Medical Research Institute, New Lambton Heights, Newcastle, NSW 2305, Australia
  • 4 Department of Pharmaceutical Technology, International Medical University, Bukit Jalil, Kuala Lumpur 57000, Malaysia
  • 5 School of Pharmacy, Suresh Gyan Vihar University, Jagatpura, Jaipur 302017, India
  • 6 School of Pharmaceutical Sciences, Shoolini University, Solan, Himachal Pradesh 173229, India
  • 7 Wits Advanced Drug Delivery Platform Research Unit, Department of Pharmacy & Pharmacology, School of Therapeutic Sciences, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg 2193, South Africa
  • 8 Centre for Inflammation, Centenary Institute, Sydney, NSW 2050, Australia
  • 9 Department of Life Sciences, School of Pharmacy, International Medical University, Bukit Jalil, Kuala Lumpur 57000, Malaysia
Nanomedicine (Lond), 2020 12;15(30):2955-2970.
PMID: 33252322 DOI: 10.2217/nnm-2020-0260

Abstract

Aim: In this study, curcumin was encapsulated in niosomes (Nio-Curc) to increase its effectiveness for the treatment of asthma. Materials & methods: The formulation underwent various physicochemical characterization experiments, an in vitro release study, molecular simulations and was evaluated for in vitro anti-inflammatory activity. Results: Results showed that Nio-Curc had a mean particle size of 284.93 ± 14.27 nm, zeta potential of -46.93 and encapsulation efficacy of 99.62%, which demonstrates optimized physicochemical characteristics. Curcumin release in vitro could be sustained for up to 24 h. Additionally, Nio-Curc effectively reduced mRNA transcript expression of pro-inflammatory markers; IL-6, IL-8, IL-1β and TNF-α in immortalized human airway basal cell line (BCi-NS1.1). Conclusion: In this study, we have demonstrated that Nio-Curc mitigated the mRNA expression of pro-inflammatory markers in an in vitro study, which could be applied to treatment of asthma with further studies.

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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