Displaying publications 21 - 40 of 57 in total

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  1. Banabilh SM, Suzina AH, Mohamad H, Dinsuhaimi S, Samsudin AR, Singh GD
    Clin Oral Investig, 2010 Oct;14(5):491-8.
    PMID: 19806371 DOI: 10.1007/s00784-009-0342-9
    The aim of the present study is to investigate nasal airway morphology in Asian adults with and without obstructive sleep apnea (OSA) using acoustic rhinometry (AR), principal components analysis (PCA), and 3-D finite-element analysis (FEA). One hundred eight adult Malays aged 18-65 years (mean ± SD, 33.2 ± 13.31) underwent clinical examination and limited channel polysomnography, providing 54 patients with OSA and 54 non-OSA controls. The mean minimal cross section area 1 (MCA1) and the mean minimal cross sectional area 2 (MCA2) were obtained from AR for all subjects and subjected to t tests. The OSA and control nasal airways were reconstructed in 3-D and subjected to PCA and FEA. The mean MCA1 and MCA2 using AR were found to be significantly smaller in the OSA group than in the control group (p < 0.001). Comparing the 3-D OSA and control nasal airways using PCA, the first two eigenvalues accounted for 94% of the total shape change, and statistical differences were found (p < 0.05). Similarly, comparing the nasal airways using FEA, the 3-D mean OSA nasal airway was significantly narrower in the OSA group compared to the control group. Specifically, decreases in size of approx. 10-22% were found in the nasal valve/head of inferior turbinate area. In conclusion, differences in nasal airway morphology are present when comparing patients with OSA to controls. These differences need to be recognized as they can improve our understanding of the etiological basis of obstructive sleep apnea and facilitate its subsequent management.
  2. Mohamad H, Lajis NH, Abas F, Ali AM, Sukari MA, Kikuzaki H, et al.
    J Nat Prod, 2005 Feb;68(2):285-8.
    PMID: 15730265
    Phytochemical studies on the rhizomes of Etlingera elatior have resulted in the isolation of 1,7-bis(4-hydroxyphenyl)-2,4,6-heptatrienone (1), demethoxycurcumin (2), 1,7-bis(4-hydroxyphenyl)-1,4,6-heptatrien-3-one (3), 16-hydroxylabda-8(17),11,13-trien-15,16-olide (4), stigmast-4-en-3-one, stigmast-4-ene-3,6-dione, stigmast-4-en-6beta-ol-3-one, and 5alpha,8alpha-epidioxyergosta-6,22-dien-3beta-ol. Compounds 1 and 4 are new, and their structures were elucidated by analysis of spectroscopic data. Diarylheptanoids 1-3 were found to inhibit lipid peroxidation in a more potent manner than alpha-tocopherol.
  3. Mustapha I, Mohd Ali B, Rasid MF, Sali A, Mohamad H
    Sensors (Basel), 2015;15(8):19783-818.
    PMID: 26287191 DOI: 10.3390/s150819783
    It is well-known that clustering partitions network into logical groups of nodes in order to achieve energy efficiency and to enhance dynamic channel access in cognitive radio through cooperative sensing. While the topic of energy efficiency has been well investigated in conventional wireless sensor networks, the latter has not been extensively explored. In this paper, we propose a reinforcement learning-based spectrum-aware clustering algorithm that allows a member node to learn the energy and cooperative sensing costs for neighboring clusters to achieve an optimal solution. Each member node selects an optimal cluster that satisfies pairwise constraints, minimizes network energy consumption and enhances channel sensing performance through an exploration technique. We first model the network energy consumption and then determine the optimal number of clusters for the network. The problem of selecting an optimal cluster is formulated as a Markov Decision Process (MDP) in the algorithm and the obtained simulation results show convergence, learning and adaptability of the algorithm to dynamic environment towards achieving an optimal solution. Performance comparisons of our algorithm with the Groupwise Spectrum Aware (GWSA)-based algorithm in terms of Sum of Square Error (SSE), complexity, network energy consumption and probability of detection indicate improved performance from the proposed approach. The results further reveal that an energy savings of 9% and a significant Primary User (PU) detection improvement can be achieved with the proposed approach.
  4. Abdullah B, Lim EH, Mohamad H, Husain S, Aziz ME, Snidvongs K, et al.
    Surg Radiol Anat, 2019 May;41(5):543-550.
    PMID: 30542929 DOI: 10.1007/s00276-018-2157-3
    PURPOSE: The variations of the anterior ethmoidal artery (AEA) in different populations should be recognized by surgeons to prevent unwarranted complications during surgery. The aim of this study was to assess the anatomical variations of AEA in Asian population.

    METHODS: A cross-sectional study of 252 AEA identified by computed tomography (CT) of the paranasal sinuses. The multiplanar CT images were acquired from SOMATOM® Definition AS+ and reconstructed to axial, coronal and sagittal view at 1 mm slice thickness.

    RESULTS: 42.5% of AEA was within skull base (grade I), 20.2% at skull base (grade II) and 37.3% coursed freely below skull base (grade III). The prevalence of supraorbital ethmoid cell (SOEC) and suprabullar cell (SBC) was 29.8% and 48.0%. The position of AEA at skull base has significant association with SOEC (p 

  5. Sundram ER, Norsa'adah B, Mohamad H, Moy FM, Husain NRN, Shafei MN
    Oman Med J, 2019 Jan;34(1):49-55.
    PMID: 30671184 DOI: 10.5001/omj.2019.08
    Objectives: We sought to determine the effectiveness of a voice care program among primary school teachers in a northeastern district in Malaysia.
    Methods: We conducted a randomized community trial in eight primary schools in a northeastern district in Malaysia. The self-administered and validated Malay-Voice Handicap Index-10 (M-VHI-10) questionnaire was used to assess overall voice handicap scores pre-intervention and eight weeks post-intervention. Teachers with a score of five or more (n = 86) were randomized into intervention (n = 41) and control groups (n = 45). The intervention group received portable voice amplifiers and vocal hygiene instruction, which was delivered by lectures and a booklet. The control group was not prescribed any intervention.
    Results: The sociodemographic, lifestyle, and occupational characteristics of the teachers (except maximum number of students per class) were similar between both groups. The baseline M-VHI-10 scores between both groups were also comparable. After the intervention phase, there was a significant effect observed in the total M-VHI-10 scores (p = 0.021, F-stat (df): 5.33 (1,79)) between both groups after controlling for the maximum number of students per class.
    Conclusions: Our results support the use of voice amplification in adjunct with vocal hygiene instruction as a prevention and treatment modality to reduce voice handicap among teachers. Our study demonstrated encouraging evidence on the low-cost voice care program as well as the success of group and workplace-based approaches in the school setting.
  6. Saad WK, Shayea I, Hamza BJ, Mohamad H, Daradkeh YI, Jabbar WA
    Sensors (Basel), 2021 Jul 31;21(15).
    PMID: 34372437 DOI: 10.3390/s21155202
    The massive growth of mobile users will spread to significant numbers of small cells for the Fifth Generation (5G) mobile network, which will overlap the fourth generation (4G) network. A tremendous increase in handover (HO) scenarios and HO rates will occur. Ensuring stable and reliable connection through the mobility of user equipment (UE) will become a major problem in future mobile networks. This problem will be magnified with the use of suboptimal handover control parameter (HCP) settings, which can be configured manually or automatically. Therefore, the aim of this study is to investigate the impact of different HCP settings on the performance of 5G network. Several system scenarios are proposed and investigated based on different HCP settings and mobile speed scenarios. The different mobile speeds are expected to demonstrate the influence of many proposed system scenarios on 5G network execution. We conducted simulations utilizing MATLAB software and its related tools. Evaluation comparisons were performed in terms of handover probability (HOP), ping-pong handover probability (PPHP) and outage probability (OP). The 5G network framework has been employed to evaluate the proposed system scenarios used. The simulation results reveal that there is a trade-off in the results obtained from various systems. The use of lower HCP settings provides noticeable enhancements compared to higher HCP settings in terms of OP. Simultaneously, the use of lower HCP settings provides noticeable drawbacks compared to higher HCP settings in terms of high PPHP for all scenarios of mobile speed. The simulation results show that medium HCP settings may be the acceptable solution if one of these systems is applied. This study emphasises the application of automatic self-optimisation (ASO) functions as the best solution that considers user experience.
  7. Saniasiaya J, Hamid SSA, Mohamad H, Wahab WNNWA, Zawawi N
    Turk Arch Otorhinolaryngol, 2019 Sep;57(3):157-160.
    PMID: 31620699 DOI: 10.5152/tao.2019.3900
    Paediatric germ cell tumor is composed of various neoplasms which exhibit capricious clinical presentation and histological features depending on the age and the area of presentation. Yolk sac tumor is an extremely rare malignant tumor of embryonic origin which usually arises from the gonads. Its manifestation in the head and neck region is extremely rare. Here, we report a rare case of the malignant transformation of mature teratoma into yolk sac tumor of the neck in an infant. Diagnosis was confirmed following histopathological examination of the neck mass along with marked increase of the serum alpha-fetoprotein. The child unfortunately succumbed prior to chemotherapy. We highlight the challenge we faced in diagnosing and managing this rare entity. We would also like to recommend serial monitoring of serum alpha-fetoprotein in all patients with mature teratoma to detect malignant transformation early.
  8. Chaiyasarn K, Ali N, Phuphasuwan P, Poovarodom N, Joyklad P, Mohamad H, et al.
    Polymers (Basel), 2021 Oct 19;13(20).
    PMID: 34685363 DOI: 10.3390/polym13203604
    Experimental and finite element analysis results of reinforced concrete beams under monotonic loading were presented in this study. In the experimental program, one beam was tested in an as-built condition. The other two beams were strengthened using natural hybrid FRP layers in different configurations. The natural hybrid FRP composite was developed by using natural jute FRP and basalt FRP. One of the most appealing advantages of natural fiber is its beneficial impact on the environment, which is necessary for the sustainability recognition as an alternative to synthetic FRP. The hybrid FRP was applied to the bottom concrete surface in one beam, while a U-shaped strengthening pattern was adopted for the other beam. The flexural behavior of each beam was assessed through strain measurements. Each beam was incorporated with conventional strain gages, as well as the Brillouin Optical Time Domain Analysis (BOTDA) technique. BOTDA has its exclusive advantages due to its simple system architecture, easy implementation, measurement speed, and cross-sensitivity. The experimental results revealed that the beam strengthened with the U-shaped hybrid FRP composite pattern had a better flexural response than the other counterpart beams did both in terms of peak loads and maximum bottom longitudinal steel bar strains. Beams B-01 and B-02 exhibited 20.5% and 28.4% higher energy dissipation capacities than the control beam did, respectively. The ultimate failure of the control beam was mainly due to the flexural cracks at very low loads, whereas the ultimate failure mode of FRP composite-strengthened beams was due to the rupture of the hybrid FRP composite. Further, strain measurements using BOTDA exhibited similar patterns as conventional strain gage measurements did. However, it was concluded that BOTDA measurements were substantially influenced by the bottom flexural cracks, ultimately resulting in shorter strain records than those of conventional strain gages. Nonlinear structural analysis of the beams was performed using the computer program ATENA. The analytical results for the control beam specimen showed a close match with the corresponding experimental results mainly in terms of maximum deflection. However, the analytical peak load was slightly higher than the corresponding experimental value.
  9. Oksal E, Pangestika I, Muhammad TST, Mohamad H, Amir H, Kassim MNI, et al.
    Saudi Pharm J, 2020 Oct;28(10):1263-1275.
    PMID: 33132720 DOI: 10.1016/j.jsps.2020.08.017
    Pandanus tectorius fruit, a natural product rich in tangeretin and ethyl caffeate, has been reported to have potential as anti-hypercholesterolemia agent via Scavenger Receptor Class B type 1 (SR-B1) pathway. However, due to its semi-polar properties, P. tectorius extract exhibits poor solubility when used as a medical remedy. The extract's solubility can potentially be improved through a synthesis of nanoparticles of chitosan-P. tectorius fruit extract. This can also increase the extract's SR-B1 gene expression activity. To date, no studies of nanoparticles of chitosan-P. tectorius fruit extract and its pathway via SR-B1 have been published anywhere. In this study, cytotoxicity properties against HepG2 were explored by MTT. Then luciferase assay was used to detect their effectiveness in increasing SR-B1 activity. An in vivo study using Sprague dawley was carried out to observe the extract nanoparticles' effectiveness in reducing the cholesterol levels and the toxicity property in rat's liver. As the results showed, the extract nanoparticles had no cytotoxic activity against HepG2 cells and exhibited higher SR-B1 gene expression activity than the non-nanoparticle form. As the in vivo study proved, nanoparticle treatment can reduce the levels of TC (197%), LDL (360%), and TG (109%), as well as increase the level of HDL cholesterol by 150%, in comparison to those for the untreated high-cholesterol diet group. From the toxicity study, it was found that there was non-toxicity in the liver. It can be concluded that nanoparticles of chitosan-P. tectorius fruit extract successfully increased P. tectorius fruit extract's effectiveness in reducing hypercholesterolemia via SR-B1 pathway. Hence, it can be suggested that nanoparticles of chitosan-P. tectorius fruit extract is safe and suitable as an alternative treatment for controlling hypercholesterolemia via SR-B1 pathway.
  10. Raj SM, Lopez D, Thambidorai CR, Kandasamy P, Toufeeq Khan TF, Mohamad H, et al.
    Singapore Med J, 1995 Aug;36(4):371-4.
    PMID: 8919148
    A survey of 142 cases of acute pancreatitis was undertaken in two major hospitals serving the state of Kelantan in Malaysia. Females outnumbered males by a ratio of more than 3:1. The incidence among females peaked in the third decade of life. Twenty-one percent (23/109) of the females were pregnant. Ultrasonography revealed gallstones in only 9.4% (13/138). However, abnormalities of serum transaminases were found in 35% (35/100), suggesting that occult gallstones or microlithiasis may be the cause in a significant proportion of patients. Alcohol was virtually absent as an aetiological factor. There was a higher frequency of Ascaris infection in this group than a control hospital population (11/35 vs 33/242; p < 0.02) suggesting that ascariasis may be an important cause of acute pancreatitis in Kelantan. Only 8.4% fell into the category of severe pancreatitis. The overall mortality rate was 2.1%.
  11. Ali M, Mohd Noor SNF, Mohamad H, Ullah F, Javed F, Abdul Hamid ZA
    Biomed Phys Eng Express, 2024 Apr 17;10(3).
    PMID: 38224615 DOI: 10.1088/2057-1976/ad1e75
    Guided tissue/bone regeneration (GTR/GBR) is a widely used technique in dentistry to facilitate the regeneration of damaged bone and tissue, which involves guiding materials that eventually degrade, allowing newly created tissue to take its place. This comprehensive review the evolution of biomaterials for guided bone regeneration that showcases a progressive shift from non-resorbable to highly biocompatible and bioactive materials, allowing for more effective and predictable bone regeneration. The evolution of biomaterials for guided bone regeneration GTR/GBR has marked a significant progression in regenerative dentistry and maxillofacial surgery. Biomaterials used in GBR have evolved over time to enhance biocompatibility, bioactivity, and efficacy in promoting bone growth and integration. This review also probes into several promising fabrication techniques like electrospinning and latest 3D printing fabrication techniques, which have shown potential in enhancing tissue and bone regeneration processes. Further, the challenges and future direction of GTR/GBR are explored and discussed.
  12. Kah Hui C, Majid NI, Mohd Yusof H, Mohd Zainol K, Mohamad H, Mohd Zin Z
    Heliyon, 2020 Jun;6(6):e04337.
    PMID: 32637711 DOI: 10.1016/j.heliyon.2020.e04337
    Cardiovascular diseases (CVDs) are silent killers and hyperlipidemia is a high-risk factor. Morinda citrolia leaf (MCL), which is commonly consumed by many cultural groups and has high level of catechins, might exert antihyperlipidemic properties. In this study, the catechins profile of MCL water extract was determined via HPLC and ultraperformance liquid chromatography-traveling wave ion mobility-quadrupole time of flight mass spectrometry (UPLC-TWIMS-QTOF). The major catechin in MCL and the most widely studied catechin with hypolipidemic activity, epigallocatechin gallate (EGCG), was studied in a cytotoxicity test on HepG2 cells prior the in vitro anti-hyperlipidemic assay. The total catechins of MCL reached 141.88 ± 5.04 mg/g, with catechin gallate (CG) (75.27 ± 8.49 mg/g) as the major catechin. Catechin derivatives that were identified include epigallocatechin-3-O-gallate (EGCG) with m/z 459.0912 [M + H]+, epigallocatechin (EGC) with m/z 307.0818 [M + H]+, CG with m/z 443.0976 [M + H]+, epigallocatechin(4β→8)-gallocatechin with m/z 649.0951 [M + K]+, and gallocatechin(4α→8)-epicatechin with m/z 633.1 [M + K]+. Cell inhibitions of MCL, CG and EGCG were more than IC50 of 100 μg/ml. MCL increased LDL-c uptake up to 1.11 ± 0.03-fold, but this was insignificant relative to control. CG and EGCG significantly increased LDL-c uptake up to 1.37 ± 0.19-fold and 1.59 ± 0.19-fold, respectively. Thus, MCL with CG has shown potential for modulating hyperlipidemia.
  13. Zamli KM, Hashim F, Razali SA, Yusoff HM, Mohamad H, Abdullah F, et al.
    Saudi Pharm J, 2023 Sep;31(9):101703.
    PMID: 37546528 DOI: 10.1016/j.jsps.2023.101703
    Amoebae of the genus Acanthamoeba can cause diseases such as amoebic keratitis and granulomatous amoebic encephalitis. Until now, treatment options for these diseases have not been fully effective and have several drawbacks. Therefore, research into new drugs is needed for more effective treatment of Acanthamoeba infections. Eugenol, a phenolic aromatic compound mainly derived from cloves, has a variety of pharmaceutical properties. In this study, nine eugenol derivatives (K1-K9), consisting of five new and four known compounds, were synthesized and screened for their antiamoebic properties against Acanthamoeba sp. The structure of these compounds was characterized spectroscopically by Fourier transform infrared (FTIR), Ultraviolet-Visible (UV-Vis), 1H and 13C Nuclear Magnetic Resonance (NMR) and mass spectrometer (MS). The derived molecules were screened for antiamoebic activity by determining IC50 values based on 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and observation of amoeba morphological changes by light and fluorescence microscopy. Most of the tested compounds possessed strong to moderate cytotoxic effects against trophozoite cells with IC50 values ranging from 0.61 to 24.83 μg/mL. Observation of amoebae morphology by light microscopy showed that the compounds caused the transformed cells to be roundish and reduced in size. Furthermore, fluorescence microscopy observation using acridine orange (AO) and propidium iodide (PI) (AO/PI) staining showed that the cells have damaged membranes by displaying a green cytoplasm with orange-stained lysosomes. Acidification of the lysosomal structure indicated disruption of the internal structure of Acanthamoeba cells when treated with eugenol derivatives. The observed biological results were also confirmed by interaction simulations based on molecular docking between eugenol derivatives and Acanthamoeba profilin. These interactions could affect the actin-binding ability of the protein, disrupting the shape and mobility of Acanthamoeba. The overall results of this study demonstrate that eugenol derivatives can be considered as potential drugs against infections caused by Acanthamoeba.
  14. Wan Draman WNA, Md Daud MK, Mohamad H, Hassan SA, Abd Rahman N
    Laryngoscope Investig Otolaryngol, 2021 Dec;6(6):1300-1306.
    PMID: 34938866 DOI: 10.1002/lio2.682
    OBJECTIVE: To determine the current microbiological profile of chronic suppurative otitis media (CSOM), their antimicrobial sensitivity, their resistance pattern to locally available antibiotics and the appropriate antibiotic against isolated microorganisms causing CSOM.

    METHODS: This cross-sectional study involved 91 ear swab specimens obtained from patients clinically diagnosed with active CSOM. Swabs were cultured for microbial identification according to a standard protocol. We performed antibiotic susceptibility testing, using the modified Kirby-Bauer disc diffusion method, and the diameter of the inhibition zone was interpreted based Clinical Laboratory Standards Institute guidelines.

    RESULTS: Microbial growth was seen in 85 (93.4%) samples, but 6 (6.6%) samples had no growth. Among the samples with growth, 63 (69.2%) were monomicrobial, 13 (14.3%) were polymicrobial, and 9 (9.9%) were of mixed growth with more than three microorganisms. The most common bacteria isolated was Pseudomonas aeruginosa (32.6%) followed by Staphylococcus aureus (16.9%) and Klebsiella spp. (5.6%). The most sensitive antibiotics against P aeruginosa were ceftazidime, meropenem, piperacillin-tazobactam, and cefepime. S aureus showed the highest sensitivity toward rifampin, cefoxitin, and fusidic acid.

    CONCLUSIONS: The bacteriological profile of CSOM showed a high prevalence of P aeruginosa, followed by S aureus and Klebsiella spp. with different distributions in different age groups. We observed a declining pattern of their antibiotic sensitivity. It is important to be aware of the current trend of the bacteriological profiles and to revise the antibiotic regime according to both the sensitivity and age groups.Level of Evidence: NA.

  15. Al-Rawi HA, Yau KL, Mohamad H, Ramli N, Hashim W
    ScientificWorldJournal, 2014;2014:960584.
    PMID: 25140350 DOI: 10.1155/2014/960584
    Cognitive radio (CR) enables unlicensed users (or secondary users, SUs) to sense for and exploit underutilized licensed spectrum owned by the licensed users (or primary users, PUs). Reinforcement learning (RL) is an artificial intelligence approach that enables a node to observe, learn, and make appropriate decisions on action selection in order to maximize network performance. Routing enables a source node to search for a least-cost route to its destination node. While there have been increasing efforts to enhance the traditional RL approach for routing in wireless networks, this research area remains largely unexplored in the domain of routing in CR networks. This paper applies RL in routing and investigates the effects of various features of RL (i.e., reward function, exploitation, and exploration, as well as learning rate) through simulation. New approaches and recommendations are proposed to enhance the features in order to improve the network performance brought about by RL to routing. Simulation results show that the RL parameters of the reward function, exploitation, and exploration, as well as learning rate, must be well regulated, and the new approaches proposed in this paper improves SUs' network performance without significantly jeopardizing PUs' network performance, specifically SUs' interference to PUs.
  16. Saddki N, Mohamad H, Mohd Yusof NI, Mohamad D, Mokhtar N, Wan Bakar WZ
    Health Qual Life Outcomes, 2013 Jun 20;11:100.
    PMID: 23786866 DOI: 10.1186/1477-7525-11-100
    BACKGROUND: The objective of this study was to determine the validity and reliability of the Malay translated Sleep Apnea Quality of Life Index (SAQLI) in patients with obstructive sleep apnea (OSA).

    METHODS: In this cross sectional study, the Malay version of SAQLI was administered to 82 OSA patients seen at the OSA Clinic, Hospital Universiti Sains Malaysia prior to their treatment. Additionally, the patients were asked to complete the Malay version of Medical Outcomes Study Short Form (SF-36). Twenty-three patients completed the Malay version of SAQLI again after 1-2 weeks to assess its reliability.

    RESULTS: Initial factor analysis of the 40-item Malay version of SAQLI resulted in four factors with eigenvalues >1. All items had factor loadings >0.5 but one of the factors was unstable with only two items. However, both items were maintained due to their high communalities and the analysis was repeated with a forced three factor solution. Variance accounted by the three factors was 78.17% with 9-18 items per factor. All items had primary loadings over 0.5 although the loadings were inconsistent with the proposed construct. The Cronbach's alpha values were very high for all domains, >0.90. The instrument was able to discriminate between patients with mild or moderate and severe OSA. The Malay version of SAQLI correlated positively with the SF-36. The intraclass correlation coefficients for all domains were >0.90.

    CONCLUSIONS: In light of these preliminary observations, we concluded that the Malay version of SAQLI has a high degree of internal consistency and concurrent validity albeit demonstrating a slightly different construct than the original version. The responsiveness of the questionnaire to changes in health-related quality of life following OSA treatment is yet to be determined.

  17. Shaari K, Ling KC, Rashid ZM, Jean TP, Abas F, Raof SM, et al.
    Mar Drugs, 2009;7(1):1-8.
    PMID: 19370166 DOI: 10.3390/md7010001
    In a preliminary screen, Aaptos aaptos showed significant cytotoxic activity towards a panel of cell lines and was thus subjected to bioassay-guided isolation of the bioactive constituents. In addition to the known aaptamine, two new derivatives of the alkaloid were isolated from the bioactive chloroform fraction of the crude methanolic extract. Detailed analysis by NMR and mass spectroscopy enabled their identification to be 3-(phenethylamino)demethyl(oxy)aaptamine and 3-(isopentylamino)demethyl(oxy) aaptamine. The cytotoxic activities of the three alkaloids were further evaluated against CEM-SS cells.
  18. Chaudhry GE, Rahman NH, Sevakumaran V, Ahmad A, Mohamad H, Zafar MN, et al.
    J Adv Pharm Technol Res, 2020 10 10;11(4):233-237.
    PMID: 33425710 DOI: 10.4103/japtr.JAPTR_81_20
    Breast cancer is among the frequently occurring cancer worldwide. The foremost underline aim of this study was to determine the growth inhibitory effect along with mechanistic study of a Bruguiera gymnorrhiza extract on MCF-7. The cytotoxicity activity was determined by using the MTS assay. Butanol extract exhibited the maximum cytotoxicity activity against the MCF-7 cells with IC50 of 3.39 μg/mL, followed by diethyl ether and methanol extract (IC50 at 16.22 μg/mL and 37.15 μg/mL, respectively) at 72 h. The DeadEndTM Colorimetric Apoptosis Detection System confirmed the induction of apoptosis (via DNA fragmentation) in MCF-7 cells. Both butanol and diethyl ether extracts of B. gymnorrhiza significantly increase the caspase-3 level. However, the diethyl ether extract induced higher caspase-9 levels compared to caspase-8, suggesting that the intrinsic pathway was the major route in the process of apoptosis. Thin-layer chromatography profiling demonstrated the presence of phenolic, terpene, and alkaloid compounds in crude methanol, diethyl ether, and butanol extracts. The phytochemicals present in the extracts of B. gymnorrhiza might have the potential to be a future therapeutic agent against breast cancer.
  19. Shehzad MT, Hameed A, Al-Rashida M, Imran A, Uroos M, Asari A, et al.
    Bioorg Chem, 2019 11;92:103244.
    PMID: 31541804 DOI: 10.1016/j.bioorg.2019.103244
    The role of aldose reductase (ALR2) in diabetes mellitus is well-established. Our interest in finding ALR2 inhibitors led us to explore the inhibitory potential of new thiosemicarbazones. In this study, we have synthesized adamantyl-thiosemicarbazones and screened them as aldehyde reductase (ALR1) and aldose reductase (ALR2) inhibitors. The compounds bearing phenyl 3a, 2-methylphenyl 3g and 2,6-dimethylphenyl 3m have been identified as most potent ALR2 inhibitors with IC50 values of 3.99 ± 0.38, 3.55 ± 0.26 and 1.37 ± 0.92 µM, respectively, compared with sorbinil (IC50 = 3.14 ± 0.02 μM). The compounds 3a, 3g, and 3m also inhibit ALR1 with IC50 value of 7.75 ± 0.28, 7.26 ± 0.39 and 7.04 ± 2.23 µM, respectively. Molecular docking was also performed for putative binding of potent inhibitors with target enzyme ALR2. The most potent 2,6-dimethylphenyl bearing thiosemicarbazone 3m (IC50 = 1.37 ± 0.92 µM for ALR2) and other two compound 3a and 3g could potentially lead for the development of new therapeutic agents.
  20. Mohamad H, Abas F, Permana D, Lajis NH, Ali AM, Sukari MA, et al.
    Z Naturforsch C J Biosci, 2005 1 26;59(11-12):811-5.
    PMID: 15666539
    The methanol extract of the dried ripe fruits of Alpinia rafflesiana was investigated for its DPPH free radical scavenger constituents. 2',3',4',6'-Tetrahydroxychalcone (7), which has never been isolated from natural sources was found to be most active as a DPPH free radical scavenger with the IC50 value of 55 microM. Other known compounds isolated from this species include 5,6-dehydrokawain (1), flavokawin B (2). 1,7-diphenyl-5-hydroxy-6-hepten-3-one (3), (-)-pinocembrin (4), cardamonin (5) and (-)-pinostrobin (6). The DPPH free radical scavenger compounds were detected using TLC autographic analysis. The percentage inhibition of DPPH free radical scavenging activity was measured on isolates (5-7) using colorimetric analysis.
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