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Influential factors in stability of lower-limb amputees

Kamali M, Karimi MT, Eshraghi A, Omar H

Am J Phys Med Rehabil, 2013 Dec;92(12):1110-8.
PMID: 23900009 DOI: 10.1097/PHM.0b013e31829b4b7a

Lower-limb amputation is mainly a result of trauma, vascular disease, diabetes, or congenital disorders. Persons with amputation lose their ability to stand and walk on the basis of the level of amputation. Contribution of level of amputation, type of amputation, or cause of amputation to balance impairment has not been clearly defined. Furthermore, it is controversial how much the mentioned parameters influence standing stability. Therefore, the aim of this review article was to find the relationship between the abovementioned factors and balance impairment in the available literature. It was also aimed to find the possibility of improving standing stability by the use of different prosthesis components.
Fulltext People living with HIV/AIDS (PLWHA) and HIV/AIDS associated oral lesions; a study in Malaysia

Khan SA, Moorthy J, Omar H, Hasan SS

BMC Public Health, 2012;12:850.
PMID: 23043358 DOI: 10.1186/1471-2458-12-850

The continuous increase in number of people living with HIV/AIDS (PLWHA) represents a serious health and economic burden. HIV positive individuals with oral lesions have significantly lower oral health-related quality of life than HIV positive individuals without oral lesions. The objective of this study was to assess the knowledge, attitude and practices (KAP) within a cohort of HIV/AIDS positive patients towards HIV/AIDS associated oral lesions.
Effect of CAD-CAM porcelain veneers thickness on their cemented color

Omar H, Atta O, El-Mowafy O, Khan SA

J Dent, 2010;38 Suppl 2:e95-9.
PMID: 20493232 DOI: 10.1016/j.jdent.2010.05.006

To determine the effect of thickness of porcelain veneers constructed from CAD-CAM on their final color when two resin cements were used.
Utilization of debate as an educational tool to learn health economics for dental students in Malaysia

Khan SA, Omar H, Babar MG, Toh CG

J Dent Educ, 2012 Dec;76(12):1675-83.
PMID: 23225687

Health economics, a special branch of science applying economic principles to the health delivery system, is a relatively young subdiscipline. The literature is scanty about teaching health economics in the medical and dental fields. Delivery methods of this topic vary from one university to another, with lectures, seminars, and independent learning reported as teaching/learning tools used for the topic. Ideally, debates should foster the development of logical reasoning and communication skills. Health economics in dentistry is taught under the community oral health module that constitutes part of an outcome-based dental curriculum in a private dental school in Kuala Lumpur, Malaysia. For this study, the students were divided into two groups: active participants (active debaters) and supporting participants (nonactive debaters). The debate style chosen for this activity was parliamentary style. Active and nonactive debaters' perceptions were evaluated before and after the activity through a structured questionnaire using a five-point rating scale addressing the topic and perceptions about debate as an educational tool. Cronbach's alpha coefficient was used as a measure of internal consistency for the questionnaire items. Among a total of eighty-two third-year dental students of two successive cohorts (thirty-eight students and forty-four students), seventy-three completed the questionnaire, yielding a response rate of 89 percent. Students' responses to the questionnaire were analyzed with the Kruskal-Wallis analysis of variance test. Results revealed that the students felt that their interest in debate, knowledge of the topic, and reinforcement of the previous knowledge had improved following participation in the debate. Within the limitations of this study, it can be concluded that debate was a useful tool in teaching health economics to dental students.
Toxic heavy metal (Pb and Cd) content in tobacco cigarette brands in Selangor state, Peninsular Malaysia

Janaydeh M, Ismail A, Zulkifli SZ, Omar H

Environ Monit Assess, 2019 Sep 16;191(10):637.
PMID: 31529162 DOI: 10.1007/s10661-019-7755-y

Cadmium (Cd) and lead (Pb) are ubiquitous metals widely distributed in the environment, resulting in toxic health effects. This project aims to evaluate Pb and Cd as toxic elements in 15 different tobacco cigarette brands produced and/or sold in Selangor state, Peninsular Malaysia. The concentrations of Pb and Cd in all tobacco brands tested in this study were determined using the air-acetylene flame atomic absorption spectrophotometer (AAS). On average, the concentrations of Pb and Cd in different tobacco brand samples ranged from 3.05 and 0.80 μg/g dw, respectively. The results indicate that assessment mean values of Pb inhaled from smoking one packet of 20 cigarettes were in the range of 1.55-3.51 μg. Furthermore, the concentration of Cd inhaled per packet of cigarettes (20 sticks) is estimated to be 0.04-0.36 μg. However, there was a significant difference in the concentrations of Pb and Cd between the different brands of tobacco cigarettes, among cigarette prices (cheap versus expensive) of tobacco brands. In conclusion, cigarette smokers in Selangor, Malaysia, are heavily exposed to Pb and Cd, and such exposure could adversely affect their health in the long term. The impact of toxic heavy metals on smokers in this state would be an area for future research.
Surface characterization analysis of failed dental implants using scanning electron microscopy

Daood U, Bandey N, Qasim SB, Omar H, Khan SA

Acta Odontol Scand, 2011 Nov;69(6):367-73.
PMID: 21449690 DOI: 10.3109/00016357.2011.569507

To investigate the failure of 15 dental implants (Paragon/Zimmer) in relation to their surface quality.
Attitudes of dental and chiropractic students towards a shared learning programme-An interprofessional learning model

Omar H, Khan S, Haneline M, Toh CG

Eur J Dent Educ, 2021 Aug;25(3):592-599.
PMID: 33222374 DOI: 10.1111/eje.12636

INTRODUCTION: Interprofessional learning (IPL) is the first stage towards the goal of interprofessional collaborative care. To enhance IPL experience, the School of Dentistry, International Medical University developed an IPL model based on the core competencies and the learning outcomes for dental and chiropractic students in their second and fourth year, respectively. The model was based on experiential learning and adult learning theories in addition to Miller's framework for clinical competencies.
METHODS: The programme was developed as a student-centred, collaborative approach to achieve the learning outcomes for dental and chiropractic students. Second-year dental students (n = 46) and chiropractic students (n = 23) in their fourth year participated in the programme. The focus of the programme was to address the prevention of work-related musculoskeletal disorders (WMSDs) amongst dental students and to provide the chiropractic students with the opportunity to assess and identify risk factors for WMSDs in the dental setting. The readiness for interprofessional learning scale (RIPLS) questionnaire was completed prior to the interprofessional education programme and once again afterwards to determine dental and chiropractic students' awareness of roles and responsibilities of the other profession, and their attitudes to interprofessional education and teamwork.
RESULTS: Dental and chiropractic students showed similar levels of readiness for shared learning. The results of this study suggest that the IPL programme contributed to the development of the students' positive perceptions towards the positive professional identity and the roles of other healthcare professionals.
CONCLUSION: This study provides initial support for the integrated interprofessional learning experiences within the school. The results of the study will shape future curricula changes to further strengthen interprofessional education and subsequent interprofessional collaborative care.
Fulltext Classification of Suncus murinus species complex (Soricidae: Crocidurinae) in Peninsular Malaysia using image analysis and machine learning approaches

Abu A, Leow LK, Ramli R, Omar H

BMC Bioinformatics, 2016 Dec 22;17(Suppl 19):505.
PMID: 28155645 DOI: 10.1186/s12859-016-1362-5

BACKGROUND: Taxonomists frequently identify specimen from various populations based on the morphological characteristics and molecular data. This study looks into another invasive process in identification of house shrew (Suncus murinus) using image analysis and machine learning approaches. Thus, an automated identification system is developed to assist and simplify this task. In this study, seven descriptors namely area, convex area, major axis length, minor axis length, perimeter, equivalent diameter and extent which are based on the shape are used as features to represent digital image of skull that consists of dorsal, lateral and jaw views for each specimen. An Artificial Neural Network (ANN) is used as classifier to classify the skulls of S. murinus based on region (northern and southern populations of Peninsular Malaysia) and sex (adult male and female). Thus, specimen classification using Training data set and identification using Testing data set were performed through two stages of ANNs.
RESULTS: At present, the classifier used has achieved an accuracy of 100% based on skulls' views. Classification and identification to regions and sexes have also attained 72.5%, 87.5% and 80.0% of accuracy for dorsal, lateral, and jaw views, respectively. This results show that the shape characteristic features used are substantial because they can differentiate the specimens based on regions and sexes up to the accuracy of 80% and above. Finally, an application was developed and can be used for the scientific community.
CONCLUSIONS: This automated system demonstrates the practicability of using computer-assisted systems in providing interesting alternative approach for quick and easy identification of unknown species.
Distribution, mobility, and pollution assessment of Cd, Cu, Ni, Pb, Zn, and Fe in intertidal surface sediments of Sg. Puloh mangrove estuary, Malaysia

Udechukwu BE, Ismail A, Zulkifli SZ, Omar H

Environ Sci Pollut Res Int, 2015 Mar;22(6):4242-55.
PMID: 25292304 DOI: 10.1007/s11356-014-3663-4

Sungai Puloh mangrove estuary supports a large diversity of macrobenthic organisms and provides social benefits to the local community. Recently, it became a major recipient of heavy metals originating from industries in the hinterland as a result of industrialization and urbanization. This study was conducted to evaluate mobility and pollution status of heavy metals (Cd, Cu, Ni, Pb, Zn, and Fe) in intertidal surface sediments of this area. Surface sediment samples were collected based on four different anthropogenic sources. Metals concentrations were analyzed using an atomic absorption spectrophotometer (AAS). Results revealed that the mean concentrations were Zn (1023.68 ± 762.93 μg/g), Pb (78.8 ± 49.61 μg/g), Cu (46.89 ± 43.79 μg/g), Ni (35.54 ± 10.75 μg/g), Cd (0.94 ± 0.29 μg/g), and Fe (7.14 ± 0.94%). Most of the mean values of analyzed metals were below both the interim sediment quality guidelines (ISQG-low and ISQG-high), except for Pb concentration (above ISQG-low) and Zn concentration (above ISQG-high), thus suggesting that Pb and Zn may pose some environmental concern. Cadmium, Pb, and Zn concentrations were above the threshold effect level (TEL), indicating seldom adverse effect of these metals on macrobenthic organisms. Pollution load index (PLI) indicated deterioration and other indices revealed the intertidal surface sediment is moderately polluted with Cd, Pb, and Zn. Therefore, this mangrove area requires urgent attention to mitigate further contamination. Finally, this study will contribute to data sources for Malaysia in establishing her own ISQG since it is a baseline study with detailed contamination assessment indices for surface sediment of intertidal mangrove area.
Fulltext Studies on the genetic variation of the green unicellular alga Haematococcus pluvialis (Chlorophyceae) obtained from different geographical locations using ISSR and RAPD molecular marker

Mostafa N, Omar H, Tan SG, Napis S

Molecules, 2011 Mar 22;16(3):2599-608.
PMID: 21441863 DOI: 10.3390/molecules16032599

Haematococcus pluvialis (Flotow) is a unicellular green alga, which is considered to be the best astaxanthin-producing organism. Molecular markers are suitable tools for the purpose of finding out genetic variations in organisms; however there have been no studies conducted on ISSR or RAPD molecular markers for this organism. The DNA of 10 different strains of H. pluvialis (four strains from Iran, two strains from Finland, one strain from Switzerland and three strains from the USA) was extracted. A genetic similarity study was carried out using 14 ISSR and 12 RAPD primers. Moreover, the molecular weights of the bands produced ranged from 0.14 to 3.4 Kb. The PCA and dendrogram clustered the H. pluvialis strains into various groups according to their geographical origin. The lowest genetic similarity was between the Iran2 and USA2 strains (0.08) and the highest genetic similarity was between Finland1 and Finland2 (0.64). The maximum numbers of bands produced by the ISSR and RAPD primers were 35 and 6 bands, respectively. The results showed that ISSR and RAPD markers are useful for genetic diversity studies of Haematococcus as they showed geographical discrimination.
Assessing changes of carbon stock in dipterocarp forest due to hydro-electric dam construction in Malaysia

Dullah H, Malek MA, Omar H, Mangi SA, Hanafiah MM

Environ Sci Pollut Res Int, 2021 Aug;28(32):44264-44276.
PMID: 33847888 DOI: 10.1007/s11356-021-13833-6

Deforestation and forest degradation are among the leading global concerns, as they could reduce the carbon sink and sequestration potential of the forest. The impoundment of Kenyir River, Hulu Terengganu, Malaysia, in 1985 due to the development of hydropower station has created a large area of water bodies following clearance of forested land. This study assessed the loss of forest carbon due to these activities within the period of 37 years, between 1972 and 2019. The study area consisted of Kenyir Lake catchment area, which consisted mainly of forests and the great Kenyir Lake. Remote sensing datasets have been used in this analysis. Satellite images from Landsat 1-5 MSS and Landsat 8 OLI/TRIS that were acquired between the years 1972 and 2019 were used to classify land uses in the entire landscape of Kenyir Lake catchment. Support vector machine (SVM) was adapted to generate the land-use classification map in the study area. The results show that the total study area includes 278,179 ha and forest covers dominated the area for before and after the impoundment of Kenyir Lake. The assessed loss of carbon between the years 1972 and 2019 was around 8.6 million Mg C with an annual rate of 0.36%. The main single cause attributing to the forest loss was due to clearing of forest for hydro-electric dam construction. However, the remaining forests surrounding the study area are still able to sequester carbon at a considerable rate and thus balance the carbon dynamics within the landscapes. The results highlight that carbon sequestration scenario in Kenyir Lake catchment area shows the potential of the carbon sink in the study area are acceptable with only 17% reduction of sequestration ability. The landscape of the study area is considered as highly vegetated area despite changes due to dam construction.
Fulltext Exposure of the endangered Milky stork population to cadmium and lead via food and water intake in Kuala Gula Bird Sanctuary, Perak, Malaysia

Rahman F, Ismail A, Omar H, Hussin MZ

Toxicol Rep, 2017;4:502-506.
PMID: 28959680 DOI: 10.1016/j.toxrep.2017.09.003

The Milky stork is listed as an endangered species endemic to the Southeast Asia region. In Malaysia, the population is currently being reintroduced back into the wild. However, the increase of anthropogenic activity throughout the coastal area might expose the population to hazardous chemicals such as heavy metals. This study highlights the contamination of cadmium (Cd) and lead (Pb) in the Milky stork's diet. Additionally, this is the first time an integrated exposure model being used to assess heavy metal exposure risk to the population. Lead level (5.5-7.98 mg kg-1) in particular was relatively high compared to Cd (0.08-0.33 mg kg-1). This was probably related to the different niches occupied by the species in the aquatic environment. The results further show that the predicted exposure doses (through intake of both food and water) for all metals are much lower than the Tolerable Daily Intake (TDI) values. The total exposure dose for Cd was 0.11 mg kg-1 d-1 with TDI value of 0.54 mg kg-1 d-1 while Pb total exposure dose was 0.31 mg kg-1 d-1 with TDI value of 0.64 mg kg-1 d-1. Several possible factors that could lead to the observed pattern were discussed. In conclusion, there is an urgent need to improve the current habitat quality to protect the endangered species. The authors also emphasized on the protection of remaining Milky stork's habitats i.e. mudflats and mangroves and the creation of buffer zone to mitigate the negative impacts that may arise from pollution activity.
Fulltext Functional data geometric morphometrics with machine learning for craniodental shape classification in shrews

Pillay AB, Pathmanathan D, Dabo-Niang S, Abu A, Omar H

Sci Rep, 2024 Jul 06;14(1):15579.
PMID: 38971911 DOI: 10.1038/s41598-024-66246-z

This work proposes a functional data analysis approach for morphometrics in classifying three shrew species (S. murinus, C. monticola, and C. malayana) from Peninsular Malaysia. Functional data geometric morphometrics (FDGM) for 2D landmark data is introduced and its performance is compared with classical geometric morphometrics (GM). The FDGM approach converts 2D landmark data into continuous curves, which are then represented as linear combinations of basis functions. The landmark data was obtained from 89 crania of shrew specimens based on three craniodental views (dorsal, jaw, and lateral). Principal component analysis and linear discriminant analysis were applied to both GM and FDGM methods to classify the three shrew species. This study also compared four machine learning approaches (naïve Bayes, support vector machine, random forest, and generalised linear model) using predicted PC scores obtained from both methods (a combination of all three craniodental views and individual views). The analyses favoured FDGM and the dorsal view was the best view for distinguishing the three species.
Prevention of mother-to-child transmission of hepatitis B virus: An observation of routine practice in a tertiary liver centre before and after the introduction of the global health sector strategy on viral hepatitis

Hoo CZ, Wan Abdullah WZ, Omar H, Tan SS

Med J Malaysia, 2023 Mar;78(2):234-240.
PMID: 36988536

INTRODUCTION: Worldwide, around 296 million people have hepatitis B virus (HBV) infection, most commonly transmitted from mother-to-child. Global Health Sector Strategy on Viral Hepatitis (GHSSVH) was introduced in May 2016, calling for elimination of viral hepatitis by 2030. This study aims to compare practice in a tertiary liver centre before and after GHSSVH introduction for prevention of mother-to-child transmission (MTCT).
MATERIALS AND METHODS: This retrospective cohort study was performed in a tertiary referral liver centre in Malaysia, using data from electronic medical record from January 2015 to December 2019. A total of 1457 medical records of female with HBV infection were screened. The inclusion criteria of the study were pregnant women with HBsAg positive or known to have HBV infection during the study period. We excluded patients with co-infections of other types of viral hepatitis or human immunodeficiency virus, concurrent liver diseases (e.g.: autoimmune hepatitis, Wilson’s disease), previous organ transplant and malignancy—except for hepatocellular carcinoma (HCC).
RESULTS: This study included 117 pregnancies and 21/117 (17.9%) were on antiviral therapy (AVT) for HBV. In 2017– 2019, 13/18 (72.2%) of those with HBV DNA >200,000IU/ml were on AVT, compared to 5/9 (55.6%) for 2015–2016, indicating 58% (95% CI −63% to 568%) higher odds of being on AVT in post GHSSVH group after accounting for HBV DNA.
CONCLUSION: Uptake of maternal AVT for the prevention of MTCT shows an increased trend since the introduction of GHSSVH, with room for improvement.
Fulltext Magnetoencephalography Phantom Comparison and Validation: Hospital Universiti Sains Malaysia (HUSM) Requisite

Omar H, Ahmad AL, Hayashi N, Idris Z, Abdullah JM

Malays J Med Sci, 2015 Dec;22(Spec Issue):20-8.
PMID: 27006634 MyJurnal

Magnetoencephalography (MEG) has been extensively used to measure small-scale neuronal brain activity. Although it is widely acknowledged as a sensitive tool for deciphering brain activity and source localisation, the accuracy of the MEG system must be critically evaluated. Typically, on-site calibration with the provided phantom (Local phantom) is used. However, this method is still questionable due to the uncertainty that may originate from the phantom itself. Ideally, the validation of MEG data measurements would require cross-site comparability.
Seasonal influence on physicochemical properties of the sediments from Bay of Bengal coast with statistical approach

Pandion K, Dowlath MJH, Arunachalam KD, Abd-Elkader OH, Yadav KK, Nazir N, et al.

Environ Res, 2023 Oct 15;235:116611.
PMID: 37437863 DOI: 10.1016/j.envres.2023.116611

The current study aims to investigate the influence of seasonal changes on the pollution loads of the sediment of a coastal area in terms of its physicochemical features. The research will focus on analyzing the nutrients, organic carbon and particle size of the sediment samples collected from 12 different sampling stations in 3 different seasons along the coastal area. Additionally, the study discusses about the impact of anthropogenic activities such as agriculture and urbanization and natural activities such as monsoon on the sediment quality of the coastal area. The nutrient changes in the sediment were found to be: pH (7.96-9.45), EC (2.89-5.23 dS/m), nitrogen (23.98-57.23 mg/kg), phosphorus (7.75-11.36 mg/kg), potassium (217-398 mg/kg), overall organic carbon (0.35-0.99%), and sediment proportions (8.91-9.3%). Several statistical methods were used to investigate changes in sediment quality. According to the three-way ANOVA test, the mean value of the sediments differs significantly with each season. It correlates significantly with principal factor analysis and cluster analysis across seasons, implying contamination from both natural and man-made sources. This study will contribute to developing effective management strategies for the protection and restoration of degraded coastal ecosystem.
Fulltext N-cyanomethylnorboldine: a new aporphine isolated from Alseodaphne perakensis (Lauraceae)

Nafiah MA, Mukhtar MR, Omar H, Ahmad K, Morita H, Litaudon M, et al.

Molecules, 2011 Apr 20;16(4):3402-9.
PMID: 21512448 DOI: 10.3390/molecules16043402

A phytochemical study of the bark of Alseodaphne perakensis has yielded three aporphine alkaloids: the new compound N-cyanomethylnorboldine (1), and the two known alkaloids N-methyllaurotetanine (2) and norboldine (3). The isolation was achieved by chromatographic techniques and the structural elucidation was performed via spectral methods, notably 1D- and 2D-NMR, UV, IR, and HRFABMS. The vasorelaxation activity of compound 1 has been studied.
Construction of cDNA library and preliminary analysis of expressed sequence tags from green microalga Ankistrodesmus convolutus Corda

Thanh T, Chi VT, Abdullah MP, Omar H, Noroozi M, Ky H, et al.

Mol Biol Rep, 2011 Jan;38(1):177-82.
PMID: 20354903 DOI: 10.1007/s11033-010-0092-4

Green microalga Ankistrodesmus convolutus Corda is a fast growing alga which produces appreciable amount of carotenoids and polyunsaturated fatty acids. To our knowledge, this is the first report on the construction of cDNA library and preliminary analysis of ESTs for this species. The titers of the primary and amplified cDNA libraries were 1.1×10(6) and 6.0×10(9) pfu/ml respectively. The percentage of recombinants was 97% in the primary library and a total of 337 out of 415 original cDNA clones selected randomly contained inserts ranging from 600 to 1,500 bps. A total of 201 individual ESTs with sizes ranging from 390 to 1,038 bps were then analyzed and the BLASTX score revealed that 35.8% of the sequences were classified as strong match, 38.3% as nominal and 25.9% as weak match. Among the ESTs with known putative function, 21.4% of them were found to be related to gene expression, 14.4% ESTs to photosynthesis, 10.9% ESTs to metabolism, 5.5% ESTs to miscellaneous, 2.0% to stress response, and the remaining 45.8% were classified as novel genes. Analysis of ESTs described in this paper can be an effective approach to isolate and characterize new genes from A. convolutus and thus the sequences obtained represented a significant contribution to the extensive database of sequences from green microalgae.
Cloning and characterization of ribulose-1,5-bisphosphate carboxylase/oxygenase small subunit (RbcS) cDNA from green microalga Ankistrodesmus convolutus

Thanh T, Chi VT, Abdullah MP, Omar H, Noroozi M, Napis S

Mol Biol Rep, 2011 Nov;38(8):5297-305.
PMID: 21287365 DOI: 10.1007/s11033-011-0679-4

An initial study on gene cloning and characterization of unicellular green microalga Ankistrodesmus convolutus was carried out to isolate and characterize the full-length cDNA of ribulose-1,5-bisphosphate carboxylase/oxygenase small subunit (RbcS) as a first step towards elucidating the structure of A. convolutus RbcS gene. The full-length of A. convolutus RbcS cDNA (AcRbcS) contained 28 bp of 5' untranslated region (UTR), 225 bp of 3' non-coding region, and an open reading frame of 165 amino acids consisting of a chloroplast transit peptide with 24 amino acids and a mature protein of 141 amino acids. The amino acid sequence has high identity to those of other green algae RbcS genes. The AcRbcS contained a few conserved domains including protein kinase C phosphorylation site, tyrosine kinase phosphorylation site and N-myristoylation sites. The AcRbcS was successfully expressed in Escherichia coli and a ~21 kDa of anticipated protein band was observed on SDS-PAGE. From the phylogenetic analysis of RbcS protein sequences, it was found that the RbcS of A. convolutus has closer genetic relationship with green microalgae species compared to those of green seaweed and green macroalgae species. Southern hybridization analysis revealed that the AcRbcS is a member of a small multigene family comprising of two to six members in A. convolutus genome. Under different illumination conditions, RT-PCR analysis showed that AcRbcS transcription was reduced in the dark, and drastically recovered in the light condition. Results presented in this paper established a good foundation for further study on the photosynthetic process of A. convolutus and other green algae species where little information is known on Rubisco small subunit.
Rapid and effective method of RNA isolation from green microalga Ankistrodesmus convolutus

Thanh T, Omar H, Abdullah MP, Chi VT, Noroozi M, Ky H, et al.

Mol Biotechnol, 2009 Oct;43(2):148-53.
PMID: 19507070 DOI: 10.1007/s12033-009-9182-8

The rapid and effective method for the isolation of RNA from green microalga Ankistrodesmus convolutus based on homogenization in a simple CTAB buffer and selective precipitation of RNA with lithium chloride is developed. This procedure avoids the use of toxic chaotropic agents and phenol while high concentration of dithiothreitol is used to inhibit RNase activity and prevent oxidative cross-linking of nucleic acids by phenolics. The extraction procedure was able to produce high quality and intact RNA from A. convolutus. The yield of total RNA was 0.69-0.73 mg/g of fresh weight, with A(260)/A(280) ratio of 1.79-1.86. The obtained RNA was of sufficient quality and suitable for downstream application such as RT-PCR and cDNA library construction. The procedure may also have wider applicability for total RNA isolation from other green microalgae species.

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