MyMedR

Phosphorylated neurofilament H (pNF-H) as a potential diagnostic marker for neurological disorders in horses

Intan-Shameha AR, Divers TJ, Morrow JK, Graves A, Olsen E, Johnson AL, et al.

Res Vet Sci, 2017 Oct;114:401-405.

PMID: 28750210 DOI: 10.1016/j.rvsc.2017.07.020

The current study aimed at the investigating the potential use of phosphorylated neurofilament H (pNF-H) as a diagnostic biomarker for neurologic disorders in the horse. Paired serum and cerebrospinal fluid (CSF) samples (n=88) and serum only (n=30) were obtained from horses diagnosed with neurologic disorders and clinically healthy horses as control. The neurologic horses consisted of equine protozoal myeloencephalitis (EPM) (38 cases) and cervical vertebral malformation (CVM) (23 cases). Levels of pNF-H were determined using an ELISA. The correlation between CSF and serum concentrations of pNF-H was evaluated using Spearman's Rank test and the significance of the difference among the groups was assessed using a nonparametric test. Horses had higher pNF-H levels in the CSF than serum. Horses afflicted with EPM had significantly higher serum pNF-H levels in comparison to controls or CVM cases. The correlation between CSF and serum pNF-H levels was poor in both the whole study population and among subgroups of horses included in the study. There was significant association between the likelihood of EPM and the concentrations of pNF-H in either the serum or CSF. These data suggest that pNF-H could be detected in serum and CSF samples from neurologic and control horses. This study demonstrated that pNF-H levels in serum and CSF have the potential to provide objective information to help in the early diagnosis of horses afflicted with neurologic disorders.

Matched MeSH terms: Encephalomyelitis/cerebrospinal fluid; Horse Diseases/cerebrospinal fluid; Nervous System Diseases/cerebrospinal fluid; Sarcocystosis/cerebrospinal fluid; Biomarkers/cerebrospinal fluid

Salmonella enteritidis ventriculitis

Johan AJ, Hung LC, Norlijah O

Southeast Asian J. Trop. Med. Public Health, 2013 May;44(3):456-9.

PMID: 24050077

Salmonella sp are important causes of meningitis among neonates and young children in Malaysia. We present a case of Salmonella enteritidis meningitis in a six week old female who presented with a one week history of fever, diarrhea and seizures which was unsuccessfully treated with a third generation cephalosporin. She had a relapse of meningitis complicated with ventriculitis and hydrocephalus, requiring an eleven week course of meropenem. She improved clinically, but did not have improvement in the cerebrospinal fluid (CSF) glucose level despite prolonged antibiotic use. This case illustrates the dilemma in determining the duration of antibiotic needed to successfully treat Salmonella enteritidis ventriculitis.

Matched MeSH terms: Cerebrospinal Fluid/immunology; Cerebrospinal Fluid/microbiology

Fulltext Proximal migration of the Hakim's valve into a porencephalic cyst

Chee Pin Chee

Med J Malaysia, 1987 Dec;42(4):309-13.

PMID: 3331410

An unusual case of proximal migration of a Hakim's valve intracranially into a porencephalic cyst two years after insertion of the ventriculo-peritoneal shunt in a neonate is reported. The underlying cause is discussed. It is recommended that all shunt should be anchored with nonabsorbable suture material properly on to the pericranium.

Matched MeSH terms: Cerebrospinal Fluid Shunts/adverse effects; Cerebrospinal Fluid Shunts/instrumentation*

Spinal pseudomeningocoele: a diagnostic dilemma

Julia PE, Nazirah H

Spinal Cord, 2007 Dec;45(12):804-5.

PMID: 17710102

Case report.

Matched MeSH terms: Encephalocele/cerebrospinal fluid; Meningocele/cerebrospinal fluid; Spinal Cord Diseases/cerebrospinal fluid

Fulltext Surgical Management of Large Occipital Encephalocoele: a case report

Sim SK, Theophilus SC, Noor Azman AR

International Medical Journal Malaysia, 2014;13(2):59-62.

MyJurnal

Cranium dysraphism occur less commonly than spinal dysraphism. Overall, occipital encephalocoeles are more frequent than anterior encephalocoes. A large occipital encephalocoele possess difficulty in surgical intervention, not only the sac contains large amount of herniated brain tissue, but the appropriate positioning for successful intubation as well as the higher rate of surgical complications such as cerebrospinal fluid leakage and skin necrosis. In this case report, surgical management of large occipital encephalocoele is discussed. The use of antibiotics in ruptured encephalocoele is recommended.

Matched MeSH terms: Cerebrospinal Fluid Leak

Fulltext Intracranial Subdural Hematoma after Lumbar Spine Surgery: A Case Report

Zakaria AF, Tsuji M

Malays Orthop J, 2019 Nov;13(3):85-87.

PMID: 31890118 DOI: 10.5704/MOJ.1911.016

Intracranial subdural hematoma following lumbar surgery is a devastating but rare complication. It has been implicated due to intracranial hypotension secondary to persistent cerebrospinal fluid leakage. The resultant drop in intracranial pressure presumably causes traction and tearing of venous structures. Patients typically present with postural headaches. However, other symptoms of subdural hematoma, intracranial hypotension and cerebrospinal fluid leak must also be cautioned.

Matched MeSH terms: Cerebrospinal Fluid Leak

Fulltext Cerebrospinal fluid rhinorrhoea secondary to amyloidosis of the sphenoid sinus

Ali EA, Philip R, Prepageran N, Peh SC

Med J Malaysia, 2008 Oct;63(4):341-2.

PMID: 19385501 MyJurnal

Amyloidosis of the skull base is a rare entity. A patient with localized amyloidosis of the sphenoid sinus presented at our institution with cerebrospinal fluid rhinorrhoea. Endoscopic excision of the lesion and multilayered obliteration of the sphenoid sinus resolved the symptoms.

Matched MeSH terms: Cerebrospinal Fluid Rhinorrhea/etiology*

Fulltext Endoscopic transnasal approach to anterior and middle cranial base lesions

Tan SH, Brand Y, Prepageran N, Waran V

Neurol India, 2015 Sep-Oct;63(5):673-80.

PMID: 26448224 DOI: 10.4103/0028-3886.166539

We present our experience in managing pathologies involving the anterior and middle cranial base using an endoscopic transnasal approach, highlighting the surgical technique, indications, and complications. The different types of endoscopic approaches used include the transtuberculum/transplanum, transcribiform, transsellar, and cavernous sinus approaches. The common indications include repair of cerebrospinal fluid leaks (both spontaneous and post traumatic) and excision of pituitary adenomas, meningiomas, craniopharyngiomas, esthesioneuroblastomas, and other malignancies of the anterior cranial base. Careful reconstruction is performed with the multilayer technique utilizing fat, fascia lata, and fibrin sealant. The endoscopic transnasal approach, coupled with the present-day sophisticated neuronavigation systems, allows access to lesions in the midline extending from the cribriform plate to the craniovertebral junction. However, preoperative planning and careful selection of cases with evaluation of each case on an individual basis with regard to the lateral extension of the lesion are imperative.

Matched MeSH terms: Cerebrospinal Fluid Rhinorrhea; Cerebrospinal Fluid Leak

Detection of Mycoplasma pneumoniae from cerebrospinal fluid samples of pediatric patients

Tay ST, Habsah MY, Tan SC, Rohani MY

Southeast Asian J. Trop. Med. Public Health, 2000 Dec;31(4):684-7.

PMID: 11414412

Central nervous system manifestations are probably the most frequent extrapulmonary complications of infections due to Mycoplasma pneumoniae, occur mostly in children. In this study, we attempted to isolate M. pneumoniae and to detect the organism by polymerase chain reaction (PCR) from cerebrospinal fluid samples (CSF) of pediatric patients. Of the 244 CSF samples, no M. pneumoniae was isolated. Six (2.5%) of the CSF samples were positive by PCR amplification. More effort are necessary to isolate the organism from CSF samples in order to ascertain the role of M. pneumoniae in causing neurological complications.

Matched MeSH terms: Mycoplasma Infections/cerebrospinal fluid*

Fulltext Per rectal extrusion of a ventriculo peritoneal shunt catheter. A case report

Ali J, Cheah FKC

Med J Malaysia, 1987 Sep;42(3):201-3.

PMID: 3333537

A rare complication of per rectal extrusion of a ventricule peritoneal shunt catheter occuring in a four-month old Chinese baby boy with hydrocephalus is described. Perforation of the bowel by the shunt occurred without any peritonitis or retrograde infection of the shunt system. Its pathogenesis and diagnosis are discussed.

Matched MeSH terms: Cerebrospinal Fluid Shunts/adverse effects*

Fulltext Isolation of salmonella from unusual sites in the body

Jegathesan M

Med J Malaysia, 1984 Dec;39(4):289-91.

PMID: 6544935

During the period 1971-1982, 57 salmonella strains other than S. typhi and S. paratyphi were isolated from sites other than stools and blood. Cerebrospinal fluid was the commonest specimen followed by urine and pus. The commonest serotypes of the 22 encountered were S. typhimurium and S. enteritidis. Twenty-four of the 57 patients were children under one year of age. These findings highlight the need to remember the unusual manifestation of salmonellosis in differential diagnoses.

Matched MeSH terms: Cerebrospinal Fluid/microbiology*

The pattern of bacteriological cultures in a state laboratory

Lim Swee Eng, Bin Mohamed Hussain AH

Med J Malaysia, 1973 Dec;28(2):103-8.

PMID: 4276266

Matched MeSH terms: Cerebrospinal Fluid/microbiology

A pseudo-cryptococcal artefact derived from leucocytes in wet India ink mounts of centrifuged cerebrospinal fluid

Thiruchelvan N, Wuu KY, Arseculeratne SN, Ashraful-Haq J

J Clin Pathol, 1998 Mar;51(3):246-8.

PMID: 9659271

Wet India ink mounts of cerebrospinal fluid (CSF) are useful in the laboratory diagnosis of cryptococcal meningitis. Pseudo-cryptococcal artefacts in such mounts have been attributed to leucocytes in CSF but their mode of formation has not been explained. This report describes the reproduction of such an artefact in cryptococcus free CSF-leucocyte mixtures that had been subjected to high speed centrifugation. The viscosity of DNA that could provide a morphological pseudo-capsule, and the yellow-green fluorescence of the pseudo-capsular material on staining with acridine-orange, suggest that lymphocytic nuclear DNA, which possibly leaked out after damage to the lymphocyte membrane by centrifugation, was responsible for this artefact.

Matched MeSH terms: Meningitis, Cryptococcal/cerebrospinal fluid

DE WITT GF,+DEWITT GF: ACTIVITY OF GLUTAMIC OXALACETIC TRANSAMINASE IN THE CEREBROSPINAL FLUID IN NEUROLOGICAL DISEASES

CHING CK

Med J Malaysia, 1963 Sep;18:8-12.

PMID: 14064302

Matched MeSH terms: Cerebrospinal Fluid*

Choroid plexus papilloma five years after shunting for hydrocephalus

Pyn CC, Arumugasamy N

Med J Malaysia, 1976 Sep;31(1):65-8.

PMID: 1023016

Matched MeSH terms: Cerebrospinal Fluid Shunts/adverse effects*

The movement of radioactive albumin (I131 R.H.S.A.) from blood into C.S.F. and vice versa by dilution method in normal and Plasmodium knowlesi infected Macaca mulatta

Migasena P, Maegraith BG

Med J Malaya, 1968 Mar;22(3):250.

PMID: 4234388

Matched MeSH terms: Serum Albumin, Radio-Iodinated/cerebrospinal fluid

Melioidosis mimicking miliary tuberculosis

Tan RZ, Mohd Nor F, Shafie S, Tan LJ

Forensic Sci Med Pathol, 2019 03;15(1):151-154.

PMID: 30293222 DOI: 10.1007/s12024-018-0026-3

Melioidosis is an infectious disease caused by Burkholderia pseudomallei, a gram-negative intracellular bacillus. Tuberculosis, also an infectious disease, is caused by Mycobacterium tuberculosis, an acid fast bacillus. In both diseases, patients commonly present with fever and respiratory symptoms due to sepsis which might lead to respiratory failure or sudden death if left untreated. Not only are these two entities similar in clinical presentation, but the autopsy findings may mimic each other, giving rise to difficulties in determining the cause of death. We report a case of melioidosis and compare it to a typical case of miliary tuberculosis. Similarities between the cases on gross and histopathological examinations are discussed. In such circumstances, microbiological culture of bodily fluids and internal organs should be performed to ascertain the correct cause of death.

Matched MeSH terms: Cerebrospinal Fluid/microbiology

Fulltext The Pathogenesis of the Demyelinating Form of Guillain-Barre Syndrome (GBS): Proteo-peptidomic and Immunological Profiling of Physiological Fluids

Ziganshin RH, Ivanova OM, Lomakin YA, Belogurov AA, Kovalchuk SI, Azarkin IV, et al.

Mol Cell Proteomics, 2016 Jul;15(7):2366-78.

PMID: 27143409 DOI: 10.1074/mcp.M115.056036

Acute inflammatory demyelinating polyneuropathy (AIDP) - the main form of Guillain-Barre syndrome-is a rare and severe disorder of the peripheral nervous system with an unknown etiology. One of the hallmarks of the AIDP pathogenesis is a significantly elevated cerebrospinal fluid (CSF) protein level. In this paper CSF peptidome and proteome in AIDP were analyzed and compared with multiple sclerosis and control patients. A total protein concentration increase was shown to be because of even changes in all proteins rather than some specific response, supporting the hypothesis of protein leakage from blood through the blood-nerve barrier. The elevated CSF protein level in AIDP was complemented by activization of protein degradation and much higher peptidome diversity. Because of the studies of the acute motor axonal form, Guillain-Barre syndrome as a whole is thought to be associated with autoimmune response against neurospecific molecules. Thus, in AIDP, autoantibodies against cell adhesion proteins localized at Ranvier's nodes were suggested as possible targets in AIDP. Indeed, AIDP CSF peptidome analysis revealed cell adhesion proteins degradation, however no reliable dependence on the corresponding autoantibodies levels was found. Proteome analysis revealed overrepresentation of Gene Ontology groups related to responses to bacteria and virus infections, which were earlier suggested as possible AIDP triggers. Immunoglobulin blood serum analysis against most common neuronal viruses did not reveal any specific pathogen; however, AIDP patients were more immunopositive in average and often had polyinfections. Cytokine analysis of both AIDP CSF and blood did not show a systemic adaptive immune response or general inflammation, whereas innate immunity cytokines were up-regulated. To supplement the widely-accepted though still unproven autoimmunity-based AIDP mechanism we propose a hypothesis of the primary peripheral nervous system damaging initiated as an innate immunity-associated local inflammation following neurotropic viruses egress, whereas the autoantibody production might be an optional complementary secondary process.

Matched MeSH terms: Autoantibodies/cerebrospinal fluid*

Fulltext Tuberculous meningitis is a major cause of mortality and morbidity in adults with central nervous system infections in Kota Kinabalu, Sabah, Malaysia: an observational study

Lee HG, William T, Menon J, Ralph AP, Ooi EE, Hou Y, et al.

BMC Infect Dis, 2016 06 16;16:296.

PMID: 27306100 DOI: 10.1186/s12879-016-1640-x

BACKGROUND: Central nervous system (CNS) infections are a significant contributor to morbidity and mortality globally. However, most published studies have been conducted in developed countries where the epidemiology and aetiology differ significantly from less developed areas. Additionally, there may be regional differences due to variation in the socio-economic levels, public health services and vaccination policies. Currently, no prospective studies have been conducted in Sabah, East Malaysia to define the epidemiology and aetiology of CNS infections. A better understanding of these is essential for the development of local guidelines for diagnosis and management.

METHODS: We conducted a prospective observational cohort study in patients aged 12 years and older with suspected central nervous system infections at Queen Elizabeth Hospital, Kota Kinabalu, Sabah, Malaysia between February 2012 and March 2013. Cerebrospinal fluid was sent for microscopy, biochemistry, bacterial and mycobacterial cultures, Mycobacterium tuberculosis polymerase chain reaction (PCR), and multiplex and MassCode PCR for various viral and bacterial pathogens.

RESULTS: A total of 84 patients with clinically suspected meningitis and encephalitis were enrolled. An aetiological agent was confirmed in 37/84 (44 %) of the patients. The most common diagnoses were tuberculous meningitis (TBM) (41/84, 48.8 %) and cryptococcal meningoencephalitis (14/84, 16.6 %). Mycobacterium tuberculosis was confirmed in 13/41 (31.7 %) clinically diagnosed TBM patients by cerebrospinal fluid PCR or culture. The acute case fatality rate during hospital admission was 16/84 (19 %) in all patients, 4/43 (9 %) in non-TBM, and 12/41 (29 %) in TBM patients respectively (p = 0.02).

CONCLUSION: TBM is the most common cause of CNS infection in patients aged 12 years or older in Kota Kinabalu, Sabah, Malaysia and is associated with high mortality and morbidity. Further studies are required to improve the management and outcome of TBM.

Matched MeSH terms: Central Nervous System Infections/cerebrospinal fluid; Meningoencephalitis/cerebrospinal fluid; Tuberculosis, Meningeal/cerebrospinal fluid; Meningitis, Cryptococcal/cerebrospinal fluid

Fulltext Multi-assay investigation of viral etiology in pediatric central nervous system infections

Altay-Kocak A, Bozdayi G, Michel J, Polat M, Kanik-Yuksek S, Tezer H, et al.

J Infect Dev Ctries, 2020 06 30;14(6):572-579.

PMID: 32683347 DOI: 10.3855/jidc.12327

INTRODUCTION: In an attempt to identify a wide spectrum of viral infections, cerebrospinal fluid (CSF) specimens were collected from pediatric cases with the preliminary diagnosis of viral encephalitis/meningoencephalitis in two reference hospitals, from October 2011 to December 2015.

METHODOLOGY: A combination of nucleic acid-based assays, including in house generic polymerase chain reaction (PCR) assays for enteroviruses, flaviviruses and phleboviruses, a commercial real-time PCR assay for herpesviruses and a commercial real time multiplex PCR, enabling detection of frequently-observed viral, bacterial and fungal agents were employed for screening.

RESULTS: The microbial agent could be characterized in 10 (10%) of the 100 specimens. Viral etiology could be demonstrated in 7 (70%) specimens, which comprises Human Herpesvirus 6 (4/7), Herpes Simplex virus type1 (2/7) and Enteroviruses (1/7). In 3 specimens (30%), Streptococcus pneumoniae, Listeria monocytogenes and Staphylococcus aureus were detected via the multiplex PCR, which were also isolated in bacteriological media. All specimens with detectable viral nucleic acids, as well as unreactive specimens via nucleic acid testing remained negative in bacteriological cultures.

CONCLUSIONS: Herpes and enteroviruses were identified as the primary causative agents of central nervous system infections in children. Enterovirus testing must be included in the diagnostic work-up of relevant cases.

Matched MeSH terms: Central Nervous System Infections/cerebrospinal fluid; Enterovirus Infections/cerebrospinal fluid; Herpesviridae Infections/cerebrospinal fluid; Virus Diseases/cerebrospinal fluid

Displaying publications 21 - 40 of 71 in total