Displaying publications 21 - 40 of 53 in total

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  1. Fulltext Development of high-throughput SNP-based genotyping in Acacia auriculiformis x A. mangium hybrids using short-read transcriptome data
    Wong MM, Cannon CH, Wickneswari R
    BMC Genomics, 2012;13:726.
    PMID: 23265623 DOI: 10.1186/1471-2164-13-726
    Next Generation Sequencing has provided comprehensive, affordable and high-throughput DNA sequences for Single Nucleotide Polymorphism (SNP) discovery in Acacia auriculiformis and Acacia mangium. Like other non-model species, SNP detection and genotyping in Acacia are challenging due to lack of genome sequences. The main objective of this study is to develop the first high-throughput SNP genotyping assay for linkage map construction of A. auriculiformis x A. mangium hybrids.
    Matched MeSH terms: Hybridization, Genetic*
  2. Identification of hybrids of painted and milky storks using FTA card-collected blood, molecular markers, and morphologies
    Yee EY, Zainuddin ZZ, Ismail A, Yap CK, Tan SG
    Biochem Genet, 2013 Oct;51(9-10):789-99.
    PMID: 23846110 DOI: 10.1007/s10528-013-9607-8
    Suspicious hybrids of painted storks and milky storks were found in a Malaysian zoo. Blood of these birds was sampled on FTA cards for DNA fingerprinting. Of 44 optimized primers, 6 produced diagnostic markers that could identify hybrids. The markers were based on simple, direct PCR-generated multilocus banding patterns that provided two sets of genetic data, one for each of the two stork species and another for the hybrids. It also revealed that large DNA fragments (3,000 bp) could be amplified from blood collected on FTA cards. When the results of each individual bird's DNA fingerprint were compared with plumage characters, the hybrids were found to express a range of intermediate phenotypic traits of the pure breeds with no dominant plumage characteristic from either parental species.
    Matched MeSH terms: Hybridization, Genetic
  3. Fulltext Induced tensile properties with EB- cross linking of hybridized kenaf/palf reinforced HDPE composite
    Aji, I.S., Zinudin, E.S., Khairul, M.Z., Abdan, K., S. M. Sapuan
    Pertanika Journal of Science & Technology, 2013;21(1):135-140.
    MyJurnal
    Electron beam irradiation, without any addition of cross-linking agents, was investigated at varying
    doses of EB-Irradiation to develop an environmentally friendly hybridized kenaf (bast)/ pineapple leaf
    fibre (PALF) bio-composites. Improvement in tensile property of the hybrid was achieved with the result
    showing a direct proportionality relationship between tensile properties and increasing radiation dose.
    Statistical analysis software (SAS) was employed to validate the result. HDPE has been shown to have
    self-cross-linked, enabling interesting tensile properties with irradiation. Statistical analysis validated
    the results obtained and also showed that adequate mixing of fibres and matrix had taken place at 95%
    confidence level. Hybridization and subsequent irradiation increased the tensile strength and modulus
    of HDPE up to 31 and 185%, respectively, at about 100kGy. Meanwhile, SEM was used to view the
    interaction between the fibres and matrix.
    Matched MeSH terms: Hybridization, Genetic
  4. Timeframes of speciation, reticulation, and hybridization in the bulldog bat explained through phylogenetic analyses of all genetic transmission elements
    Khan FA, Phillips CD, Baker RJ
    Syst Biol, 2014 Jan 1;63(1):96-110.
    PMID: 24149076 DOI: 10.1093/sysbio/syt062
    Phylogenetic comparisons of the different mammalian genetic transmission elements (mtDNA, X-, Y-, and autosomal DNA) is a powerful approach for understanding the process of speciation in nature. Through such comparisons the unique inheritance pathways of each genetic element and gender-biased processes can link genomic structure to the evolutionary process, especially among lineages which have recently diversified, in which genetic isolation may be incomplete. Bulldog bats of the genus Noctilio are an exemplar lineage, being a young clade, widely distributed, and exhibiting unique feeding ecologies. In addition, currently recognized species are paraphyletic with respect to the mtDNA gene tree and contain morphologically identifiable clades that exhibit mtDNA divergences as great as among many species. To test taxonomic hypotheses and understand the contribution of hybridization to the extant distribution of genetic diversity in Noctilio, we used phylogenetic, coalescent stochastic modeling, and divergence time estimates using sequence data from cytochrome-b, cytochrome c oxidase-I, zinc finger Y, and zinc finger X, as well as evolutionary reconstructions based on amplified fragment length polymorphisms (AFLPs) data. No evidence of ongoing hybridization between the two currently recognized species was identified. However, signatures of an ancient mtDNA capture were recovered in which an mtDNA lineage of one species was captured early in the noctilionid radiation. Among subspecific mtDNA clades, which were generally coincident with morphology and statistically definable as species, signatures of ongoing hybridization were observed in sex chromosome sequences and AFLP. Divergence dating of genetic elements corroborates the diversification of extant Noctilio beginning about 3 Ma, with ongoing hybridization between mitochondrial lineages separated by 2.5 myr. The timeframe of species' divergence within Noctilio supports the hypothesis that shifts in the dietary strategies of gleaning insects (N. albiventris) or fish (N. leporinus) are among the most rapid instances of dietary evolution observed in mammals. This study illustrates the complex evolutionary dynamics shaping gene pools in nature, how comparisons of genetic elements can serve for understanding species boundaries, and the complex considerations for accurate taxonomic assignment.
    Matched MeSH terms: Hybridization, Genetic*
  5. Fulltext Isolation and characterization of CCoAOMT in interspecific hybrid of Acacia auriculiformis x Acacia mangium--a key gene in lignin biosynthesis
    Pang SL, Ong SS, Lee HH, Zamri Z, Kandasamy KI, Choong CY, et al.
    Genet. Mol. Res., 2014;13(3):7217-38.
    PMID: 25222227 DOI: 10.4238/2014.September.5.7
    This study was directed at the understanding of the function of CCoAOMT isolated from Acacia auriculiformis x Acacia mangium. Full length cDNA of the Acacia hybrid CCoAOMT (AhCCoAOMT) was 1024-bp long, containing 750-bp coding regions, with one major open reading frame of 249 amino acids. On the other hand, full length genomic sequence of the CCoAOMT (AhgflCCoAOMT) was 2548 bp long, containing three introns and four exons with a 5' untranslated region (5'UTR) of 391 bp in length. The 5'UTR of the characterized CCoAOMT gene contains various regulatory elements. Southern analysis revealed that the Acacia hybrid has more than three copies of the CCoAOMT gene. Real-time PCR showed that this gene was expressed in root, inner bark, leaf, flower and seed pod of the Acacia hybrid. Downregulation of the homologous CCoAOMT gene in tobacco by antisense (AS) and intron-containing hairpin (IHP) constructs containing partial AhCCoAOMT led to reduction in lignin content. Expression of the CCoAOMT in AS line (pART-HAS78-03) and IHP line (pART-HIHP78-06) was reduced respectively by 37 and 75% compared to the control, resulting in a decrease in the estimated lignin content by 24 and 56%, respectively. AhCCoAOMT was found to have altered not only S and G units but also total lignin content, which is of economic value to the pulp industry. Subsequent polymorphism analysis of this gene across eight different genetic backgrounds each of A. mangium and A. auriculiformis revealed 47 single nucleotide polymorphisms (SNPs) in A. auriculiformis CCoAOMT and 30 SNPs in A. mangium CCoAOMT.
    Matched MeSH terms: Hybridization, Genetic*
  6. Fulltext A classical genetic solution to enhance the biosynthesis of anticancer phytochemicals in Andrographis paniculata Nees
    Valdiani A, Talei D, Tan SG, Abdul Kadir M, Maziah M, Rafii MY, et al.
    PLoS One, 2014;9(2):e87034.
    PMID: 24586262 DOI: 10.1371/journal.pone.0087034
    Andrographolides, the diterpene lactones, are major bioactive phytochemicals which could be found in different parts of the medicinal herb Andrographis paniculata. A number of such compounds namely andrographolide (AG), neoandrographolide (NAG), and 14-deoxy-11,12-didehydroandrographolide (DDAG) have already attracted a great deal of attention due to their potential therapeutic effects in hard-to-treat diseases such as cancers and HIV. Recently, they have also been considered as substrates for the discovery of novel pharmaceutical compounds. Nevertheless, there is still a huge gap in knowledge on the genetic pattern of the biosynthesis of these bioactive compounds. Hence, the present study aimed to investigate the genetic mechanisms controlling the biosynthesis of these phytochemicals using a diallel analysis. The high performance liquid chromatography analysis of the three andrographolides in 210 F1 progenies confirmed that the biosynthesis of these andrographolides was considerably increased via intraspecific hybridization. The results revealed high, moderate and low heterosis for DDAG, AG and NAG, respectively. Furthermore, the preponderance of non-additive gene actions was affirmed in the enhancement of the three andrographolides contents. The consequence of this type of gene action was the occurrence of high broad-sense and low narrow-sense heritabilities for the above mentioned andrographolides. The prevalence of non-additive gene action suggests the suitability of heterosis breeding and hybrid seed production as a preferred option to produce new plant varieties with higher andrographolide contents using the wild accessions of A. paniculata. Moreover, from an evolutionary point of view, the occurrence of population bottlenecks in the Malaysian accessions of A. paniculata was unveiled by observing a low level of additive genetic variance (VA ) for all the andrographolides.
    Matched MeSH terms: Hybridization, Genetic/genetics
  7. Interspecific Cross-Mating Between Aedes aegypti and Aedes albopictus Laboratory Strains: Implication of Population Density on Mating Behaviors
    Marcela P, Hassan AA, Hamdan A, Dieng H, Kumara TK
    J Am Mosq Control Assoc, 2015 Dec;31(4):313-20.
    PMID: 26675452 DOI: 10.2987/moco-31-04-313-320.1
    Mating behavior between Aedes aegypti and Ae. albopictus, established colony strains were examined under laboratory conditions (30-cm(3) screened cages) for 5 consecutive days. The effect of selected male densities (30, 20, 10) and female density (20) on the number of swarming, mating pairs, eggs produced, and inseminated females were evaluated. Male densities significantly increased swarming behavior, mating pairs, and egg production of heterospecific females, but female insemination was reduced. Aedes aegypti males mate more readily with heterospecific females than do Ae. albopictus males. The current study suggests that Ae. aegypti males were not species-specific in mating, and if released into the field as practiced in genetically modified mosquito techniques, they may mate with both Ae. aegypti and Ae. albopictus females, hence reducing populations of both species by producing infertile eggs.
    Matched MeSH terms: Hybridization, Genetic*
  8. Fulltext dsRNA silencing of an R2R3-MYB transcription factor affects flower cell shape in a Dendrobium hybrid
    Lau SE, Schwarzacher T, Othman RY, Harikrishna JA
    BMC Plant Biol, 2015;15:194.
    PMID: 26260631 DOI: 10.1186/s12870-015-0577-3
    The R2R3-MYB genes regulate pigmentation and morphogenesis of flowers, including flower and cell shape, and therefore have importance in the development of new varieties of orchids. However, new variety development is limited by the long breeding time required in orchids. In this study, we identified a cDNA, DhMYB1, that is expressed during flower development in a hybrid orchid, Dendrobium hybrida (Dendrobium bobby messina X Dendrobium chao phraya) then used the direct application of dsRNA to observe the effect of gene silencing on flower phenotype and floral epidermal cell shape.
    Matched MeSH terms: Hybridization, Genetic
  9. Fulltext A comparative study on standard, modified and chaotic firefly algorithms
    Hong, Choon Ong, Tilahun, Surafel Luleseged, Tang, Suey Shya
    Pertanika Journal of Science & Technology, 2015;23(2):251-269.
    MyJurnal
    Many studies have been carried out using different metaheuristic algorithms on optimisation problems in various fields like engineering design, economics and routes planning. In the real world, resources and time are scarce. Thus the goals of optimisation algorithms are to optimise these available resources. Different metaheuristic algorithms are available. The firefly algorithm is one of the recent metaheuristic algorithms that is used in many applications; it is also modified and hybridised to improve its performance. In this paper, we compare the Standard Firefly Algorithm, the Elitist Firefly Algorithm, also called the Modified Firefly Algorithm with the Chaotic Firefly Algorithm, which embeds chaos maps in the Standard Firefly Algorithm. The Modified Firefly Algorithm differs from the Standard Firefly Algorithm in such a way that the global optimum solution at a particular iteration will not move randomly but in a direction that is chosen from randomly generated directions that can improve its performance. If none of these directions improves its performance, then the algorithm will not be updated. On the other hand, the Chaotic Firefly Algorithm tunes the parameters of the algorithms for the purpose of increasing the global search mobility i.e. to improve the attractiveness of fireflies. In our study, we found that the Chaotic Firefly Algorithms using three different chaotic maps do not perform as well as the Modified Firefly Algorithms; however, at least one or two of the Chaotic Firefly Algorithms outperform the Standard Firefly Algorithm under the given accuracy and efficiency tests.
    Matched MeSH terms: Hybridization, Genetic
  10. Morphological and molecular characterization of yellow oyster mushroom, Pleurotus citrinopileatus, hybrids obtained by interspecies mating
    Rosnina AG, Tan YS, Abdullah N, Vikineswary S
    World J Microbiol Biotechnol, 2016 Feb;32(2):18.
    PMID: 26745978 DOI: 10.1007/s11274-015-1959-2
    Pleurotus citrinopileatus (yellow oyster mushroom) has an attractive shape and yellow colour but the fragile texture complicates packaging, and its strong aroma is unappealing to consumers. This study aimed to improve the characteristics and yield of P. citrinopileatus by interspecies mating between monokaryotic cultures of P. citrinopileatus and P. pulmonarius. Ten monokaryon cultures of the parental lines were crossed in all combinations to obtain hybrids. Eleven compatible mating pairs were obtained and cultivated to observe their sporophore morphology and yield. The selected hybrid, i.e. P1xC9, was beige in colour while hybrid P3xC8 was yellow in colour. Their sporophores had less offensive aroma, improved texture and higher yield. The DNA sequences of these hybrids were found to be in the same clade as the P. citrinopileatus parent with a bootstrap value of 99%. High bootstrap values indicate high genetic homology between hybrids and the P. citrinopileatus parent. The biological efficiencies of these hybrids P1xC9 (70.97%) and P3xC8 (52.14%) were also higher than the P. citrinopileatus parent (35.63%). Interspecies hybrids obtained by this mating technique can lead to better strains of mushrooms for genetic improvement of the Pleurotus species.
    Matched MeSH terms: Hybridization, Genetic*
  11. A Natural Population Derived from Species Hybridizationin the Drosophila ananassae Species Complexon Penang Island, Malaysia
    Sawamura K, Sato H, Lee CY, Kamimura Y, Matsuda M
    Zoolog Sci, 2016 Oct;33(5):467-475.
    PMID: 27715417
    We surveyed natural population of the Drosophila ananassae species complex on Penang Island, Malaysia. Analyses of phenotypic traits, chromosome arrangements, molecular markers, and reproductive isolation suggest the existence of two species: D. ananassae and D. cf. parapallidosa. Molecular marker analysis indicates that D. cf. parapallidosa carries chromosome Y and 4 introgressions from D. ananassae. Thus, D. cf. parapallidosa seems to be a hybrid descendant that recently originated from a natural D. parapallidosa♀× D. ananassae♂ cross. Furthermore, D. cf. parapallidosa behaves differently from authentic D. parapallidosa with respect to its reproductive isolation from D. ananassae. Premating isolation is usually seen in only the D. ananassae♀× D. parapallidosa♂ cross, but we observed it in crosses of both directions between D. ananassae and D. cf. parapallidosa. In addition, hybrid males from the D. ananassae♀× D. parapallidosa♂ cross are usually sterile, but they were fertile when D. ananassae♀ were mated with D. cf. parapallidosa ♂. We attempted an artificial reconstruction of the hybrid species to simulate the evolutionary process(es) that produced D. cf. parapallidosa. This is a rare case of natural hybrid population in Drosophila and may be a useful system for elucidating speciation with gene flow.
    Matched MeSH terms: Hybridization, Genetic
  12. The effect of feed-based vaccination on tilapia farm endemic for streptococcosis
    Ismail MS, Syafiq MR, Siti-Zahrah A, Fahmi S, Shahidan H, Hanan Y, et al.
    Fish Shellfish Immunol, 2017 Jan;60:21-24.
    PMID: 27864157 DOI: 10.1016/j.fsi.2016.11.040
    A tilapia farm experiencing endemic streptococcosis was selected to study the effect of vaccination with a feed-based vaccine on naturally ocurring streptococcosis. A total of 9000 red tilapia, Oreochromis niloticus × Oreochromis mossambicus of 100 ± 20 g were divided into 9 cages. Fish of Group 1 in cages 1, 2 and 3 were not vaccinated. Group 2 in cages 4, 5 and 6 were vaccinated on days 0 and 14 (single booster) while Group 3 in cages 7, 8 and 9 were vaccinated on days 0, 14 and 42 (double booster). Vaccination was done by oral administration of the feed-based bacterin vaccine at 4% bodyweight. Samples of serum for antibody study and the brain, eyes and kidney for bacterial isolation were collected at 14-day intervals. The study was carried out during the critical months between April and June. Following vaccination and booster, there was significant (p 
    Matched MeSH terms: Hybridization, Genetic
  13. Fulltext Phylogeography of the smooth-coated otter (Lutrogale perspicillata): distinct evolutionary lineages and hybridization with the Asian small-clawed otter (Aonyx cinereus)
    Moretti B, Al-Sheikhly OF, Guerrini M, Theng M, Gupta BK, Haba MK, et al.
    Sci Rep, 2017 Jan 27;7:41611.
    PMID: 28128366 DOI: 10.1038/srep41611
    We investigated the phylogeography of the smooth-coated otter (Lutrogale perspicillata) to determine its spatial genetic structure for aiding an adaptive conservation management of the species. Fifty-eight modern and 11 archival (dated 1882-1970) otters sampled from Iraq to Malaysian Borneo were genotyped (mtDNA Cytochrome-b, 10 microsatellite DNA loci). Moreover, 16 Aonyx cinereus (Asian small-clawed otter) and seven Lutra lutra (Eurasian otter) were sequenced to increase information available for phylogenetic reconstructions. As reported in previous studies, we found that L. perspicillata, A. cinereus and A. capensis (African clawless otter) grouped in a clade sister to the genus Lutra, with L. perspicillata and A. cinereus being reciprocally monophyletic. Within L. perspicillata, we uncovered three Evolutionarily Significant Units and proved that L. p. maxwelli is not only endemic to Iraq but also the most recent subspecies. We suggest a revision of the distribution range limits of easternmost L. perspicillata subspecies. We show that smooth-coated otters in Singapore are L. perspicillata x A. cinereus hybrids with A. cinereus mtDNA, the first reported case of hybridization in the wild among otters. This result also provides evidence supporting the inclusion of L. perspicillata and A. cinereus in the genus Amblonyx, thus avoiding the paraphyly of the genus Aonyx.
    Matched MeSH terms: Hybridization, Genetic*
  14. Fulltext A Cross-Species Gene Expression Marker-Based Genetic Map and QTL Analysis in Bambara Groundnut
    Chai HH, Ho WK, Graham N, May S, Massawe F, Mayes S
    Genes (Basel), 2017 Feb 22;8(2).
    PMID: 28241413 DOI: 10.3390/genes8020084
    Bambara groundnut (Vigna subterranea (L.) Verdc.) is an underutilised legume crop, which has long been recognised as a protein-rich and drought-tolerant crop, used extensively in Sub-Saharan Africa. The aim of the study was to identify quantitative trait loci (QTL) involved in agronomic and drought-related traits using an expression marker-based genetic map based on major crop resources developed in soybean. The gene expression markers (GEMs) were generated at the (unmasked) probe-pair level after cross-hybridisation of bambara groundnut leaf RNA to the Affymetrix Soybean Genome GeneChip. A total of 753 markers grouped at an LOD (Logarithm of odds) of three, with 527 markers mapped into linkage groups. From this initial map, a spaced expression marker-based genetic map consisting of 13 linkage groups containing 218 GEMs, spanning 982.7 cM (centimorgan) of the bambara groundnut genome, was developed. Of the QTL detected, 46% were detected in both control and drought treatment populations, suggesting that they are the result of intrinsic trait differences between the parental lines used to construct the cross, with 31% detected in only one of the conditions. The present GEM map in bambara groundnut provides one technically feasible route for the translation of information and resources from major and model plant species to underutilised and resource-poor crops.
    Matched MeSH terms: Hybridization, Genetic
  15. Fulltext Ancestry, Plasmodium cynomolgi prevalence and rhesus macaque admixture in cynomolgus macaques (Macaca fascicularis) bred for export in Chinese breeding farms
    Zhang X, Meng Y, Houghton P, Liu M, Kanthaswamy S, Oldt R, et al.
    J Med Primatol, 2017 04;46(2):31-41.
    PMID: 28266719 DOI: 10.1111/jmp.12256
    BACKGROUND: Most cynomolgus macaques (Macaca fascicularis) used in the United States as animal models are imported from Chinese breeding farms without documented ancestry. Cynomolgus macaques with varying rhesus macaque ancestry proportions may exhibit differences, such as susceptibility to malaria, that affect their suitability as a research model.

    METHODS: DNA of 400 cynomolgus macaques from 10 Chinese breeding farms was genotyped to characterize their regional origin and rhesus ancestry proportion. A nested PCR assay was used to detect Plasmodium cynomolgi infection in sampled individuals.

    RESULTS: All populations exhibited high levels of genetic heterogeneity and low levels of inbreeding and genetic subdivision. Almost all individuals exhibited an Indochinese origin and a rhesus ancestry proportion of 5%-48%. The incidence of P. cynomolgi infection in cynomolgus macaques is strongly associated with proportion of rhesus ancestry.

    CONCLUSIONS: The varying amount of rhesus ancestry in cynomolgus macaques underscores the importance of monitoring their genetic similarity in malaria research.

    Matched MeSH terms: Hybridization, Genetic
  16. First report on the successful hybridization of Pangasianodon hypophthalmus (Sauvage, 1878) and Clarias gariepinus (Burchell, 1822)
    Okomoda VT, Koh ICC, Shahreza MS
    Zygote, 2017 Aug;25(4):443-452.
    PMID: 28635581 DOI: 10.1017/S0967199417000259
    Breeding and larval performance of novel hybrids from reciprocal crosses of Asian catfish Pangasianodon hypophthalmus (Sauvage, 1878) and African catfish Clarias gariepinus (Burchell, 1822) were investigated in this study. Spawning was by hormonal injection of brood fish, artificial fertilization, and incubation in triplicate aquarium tanks (0.5 × 0.5 × 0.5 m3) with continuous aeration. Reciprocal crosses (♀C. gariepinus × ♂P. hypophthalmus and ♀P. hypophthalmus × ♂C. gariepinus) had lower hatchability (≤50%) than their pure siblings (≥75%). Fish from all crosses survived until the juvenile stage but survival at 35 days post hatching (dph) was higher for pure C. gariepinus sib. ♀C. gariepinus × ♂P. hypophthalmus was observed to be less resistant to degradation of water quality than the other crosses, however it had higher body weight compared with the other crosses that showed similar performance. Morphological comparison of surviving juvenile at 35 dph, showed that all ♀P. hypophthalmus × ♂C. gariepinus and 13% of the ♀C. gariepinus × ♂P. hypophthalmus exhibited the very same morphology as that of their maternal parent species, while the other portion of the ♀C. gariepinus × ♂P. hypophthalmus cross exhibited morphological traits that were intermediate between those of both parent species. This study been the first successful attempt to hybridize both species and therefore, laid the groundwork for further studies on the aquaculture potentials of the novel hybrids.
    Matched MeSH terms: Hybridization, Genetic
  17. ISSR marker-assisted genetic diversity analysis of Dioscorea hispida and selection of the best variety for sustainable production
    Nudin NFH, Ali AM, Ngah N, Mazlan NZ, Mat N, Ghani MNA, et al.
    C. R. Biol., 2017 Aug;340(8):359-366.
    PMID: 28888550 DOI: 10.1016/j.crvi.2017.08.003
    Plant breeding is a way of selection of a particular individual for the production of the progeny by separating or combining desired characteristics. The objective of this study was to justify different characteristics of Dioscorea hispida (Ubi gadong) varieties using molecular techniques to select the best variety for sustainable production at the farmer's level. A total of 160 germplasms of Ubi gadong were collected from different locations at the Terengganu and Kelantan states of Malaysia. Forty eight (48) out of 160 germplasms were selected as "primary" selection based on yield and other qualitative characters. Selected collections were then grown and maintained for ISSR marker-assisted genetic diversity analysis. Overall plant growth and yield of tubers were also determined. A total of 12 ISSR markers were tested to justify the characteristics of Ubi gadong varieties among which three markers showed polymorphic bands and on average 57.3% polymorphism were observed representing the highest variation among germplasms. The ISSR marker based on UPGMA cluster analysis grouped all 48 D. hispida into 10 vital groups that proved a vast genetic variation among germplasm collections. Therefore, hybridization should be made between two distant populations. The D. hispida is already proved as the highest starch content tuber crops and very rich in vitamins with both micro and macro minerals. Considering all these criteria and results from marker-assisted diversity analysis, accessions that are far apart based on their genetic coefficient (like DH27 and DH71; DH30 and DH70; DH43 and DH62; DH45 and DH61; DH77 and DH61; DH78 and DH57) could be selected as parents for further breeding programs. This will bring about greater diversity, which will lead to high productive index in terms of increase in yield and overall quality and for the ultimate target of sustainable Ubi gadong production.
    Matched MeSH terms: Hybridization, Genetic
  18. Fulltext Mass spectrometry data of metabolomics analysis of Nepenthes pitchers
    Rosli MAF, Azizan KA, Baharum SN, Goh HH
    Data Brief, 2017 Oct;14:295-297.
    PMID: 28795107 DOI: 10.1016/j.dib.2017.07.068
    Hybridisation plays a significant role in the evolution and diversification of plants. Hybridisation among Nepenthes species is extensive, either naturally or man-made. To investigate the effects of hybridisation on the chemical compositions, we carried out metabolomics study on pitcher tissue of Nepenthes ampullaria, Nepenthes rafflesiana and their hybrid, Nepenthes × hookeriana. Pitcher samples were harvested and extracted in methanol:chloroform:water via sonication-assisted extraction before analysed using LC-TOF-MS. MS data were analysed using XCMS online version 2.2.5. This is the first MS data report towards the profiling, identification and comprehensive comparison of metabolites present in Nepenthes species.
    Matched MeSH terms: Hybridization, Genetic
  19. Genetic analysis of samples from wild populations opens new perspectives on hybridization between long-tailed (Macaca fascicularis) and rhesus macaques (Macaca mulatta)
    Bunlungsup S, Kanthaswamy S, Oldt RF, Smith DG, Houghton P, Hamada Y, et al.
    Am J Primatol, 2017 12;79(12).
    PMID: 29095514 DOI: 10.1002/ajp.22726
    In the past decade, many researchers have published papers about hybridization between long-tailed and rhesus macaques. These previous works have proposed unidirectional gene flow with the Isthmus of Kra as the zoogeographical barrier of hybridization. However, these reports analyzed specimens of unknown origin and/or did not include specimens from Thailand, the center of the proposed area of hybridization. Collected specimens of long-tailed and rhesus macaques representing all suspected hybridization areas were examined. Blood samples from four populations each of long-tailed and rhesus macaques inhabiting Thailand, Myanmar, and Laos were collected and analyzed with conspecific references from China (for rhesus macaques) and multiple countries from Sundaic regions (for long-tailed macaques). Ninety-six single nucleotide polymorphism (SNP) markers specifically designed to interrogate admixture and ancestry were used in genotyping. We found genetic admixture maximized at the hybrid zone (15-20°N), as well as admixture signals of varying strength in both directions outside of the hybrid zone. These findings show that the Isthmus of Kra is not a barrier to gene flow from rhesus to long-tailed populations. However, to precisely identify a southernmost barrier, if in fact a boundary rather than simple isolation by distance exists, the samples from peninsular Malaysia must be included in the analysis. Additionally, a long-tailed to rhesus gene flow boundary was found between northern Thailand and Myanmar. Our results suggest that selection of long-tailed and rhesus macaques, the two most commonly used non-human primates for biomedical research, should take into account not only the species identification but also the origin of and genetic admixture within and between the species.
    Matched MeSH terms: Hybridization, Genetic*
  20. One-pot green synthesis of highly reduced graphene oxide decorated with silver nanoparticles
    Nur Suhaili Abd Aziz, Muhammad Khairullah Nor Azmi, Abdul Manaf Hashim
    Sains Malaysiana, 2017;46:1083-1088.
    A one-pot green sonochemical process assisted by ascorbic acid as the reducing agent to produce highly reduced graphene oxide (rGO) decorated with silver nanoparticles (AgNPs) is demonstrated. A complete removal of oxygen-containing group in the GO sheets was confirmed by no observation of the peak corresponds to C-O, C=O and -OH bond. The unexpected decrease of peak intensity corresponds to sp2 hybridized C=C group is explained by a so-called bond polarity effect. The peak observed at ~400 nm seems to show the presence of AgNPs and the red shifting of C=C peak to ~270 nm after the introduction of ascorbic acid indicates the formation of highly reduced GO. The increase of AgNPs size and the crumpled silk-like morphology after the introduction of ascorbic acid also indicate the aggressive reduction of both AgNPs and GO. The increase of ID/IG ratio after the introduction of ascorbic acid seems to indicate the increase of the number of small sp2 domains, the presence of unrepaired defects and the restoration of the sp2 network. This work provides the promising green sonochemical approach by utilizing non-toxic and environmental-friendly reducing agent to produce highly reduced GO decorated with AgNPs for various applications.
    Matched MeSH terms: Hybridization, Genetic
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