Displaying publications 21 - 40 of 296 in total

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  1. Amran M, Fediuk R, Vatin N, Lee YH, Murali G, Ozbakkaloglu T, et al.
    Materials (Basel), 2020 Sep 28;13(19).
    PMID: 32998362 DOI: 10.3390/ma13194323
    Foamed concrete (FC) is a high-quality building material with densities from 300 to 1850 kg/m3, which can have potential use in civil engineering, both as insulation from heat and sound, and for load-bearing structures. However, due to the nature of the cement material and its high porosity, FC is very weak in withstanding tensile loads; therefore, it often cracks in a plastic state, during shrinkage while drying, and also in a solid state. This paper is the first comprehensive review of the use of man-made and natural fibres to produce fibre-reinforced foamed concrete (FRFC). For this purpose, various foaming agents, fibres and other components that can serve as a basis for FRFC are reviewed and discussed in detail. Several factors have been found to affect the mechanical properties of FRFC, namely: fresh and hardened densities, particle size distribution, percentage of pozzolanic material used and volume of chemical foam agent. It was found that the rheological properties of the FRFC mix are influenced by the properties of both fibres and foam; therefore, it is necessary to apply an additional dosage of a foam agent to enhance the adhesion and cohesion between the foam agent and the cementitious filler in comparison with materials without fibres. Various types of fibres allow the reduction of by autogenous shrinkage a factor of 1.2-1.8 and drying shrinkage by a factor of 1.3-1.8. Incorporation of fibres leads to only a slight increase in the compressive strength of foamed concrete; however, it can significantly improve the flexural strength (up to 4 times), tensile strength (up to 3 times) and impact strength (up to 6 times). At the same time, the addition of fibres leads to practically no change in the heat and sound insulation characteristics of foamed concrete and this is basically depended on the type of fibres used such as Nylon and aramid fibres. Thus, FRFC having the presented set of properties has applications in various areas of construction, both in the construction of load-bearing and enclosing structures.
    Matched MeSH terms: Plastics
  2. Andreas, Hadibarata T, Sathishkumar P, Prasetia H, Hikmat, Pusfitasari ED, et al.
    Chemosphere, 2021 Aug;276:130185.
    PMID: 33743420 DOI: 10.1016/j.chemosphere.2021.130185
    Indonesia is the second-largest contributor of microplastics (MPs) pollution in the marine ecosystem. Most MPs pollution-related studies in Indonesia focus on seawater, sediment, with less information found on the commercially important fish species used for human consumption. Skipjack Tuna (Euthynnus affinis) is one of the major exporting fishery commodities from Indonesia. This exploratory study aimed to determine MPs presence in the digestive tract of Skipjack Tuna from the Southern Coast of Java, Indonesia. The fish samples were collected from five different fish traditional auction market along the Southern Coast of Java, Indonesia, namely Pangandaran, Pamayang Sari, Ciletuh, Santolo, and Palabuhan Ratu. The gastrointestinal tract of Skipjack tuna was pretreated using alkaline destruction and filtered. The presence of MPs in the treated samples was visually identified using an optical microscope, while Polybrominated diphenyl ethers (PBDEs) contaminants were analyzed using Gas Chromatography-Mass Spectrometry (GC-MS). A total of 19 suspected MPs particles were found in the form of filament (84%), angular (11%), and round (5%). This result would provide a better indication of the MPs contamination in marine life species in the Southern Coast of Java, Indonesia, as useful information for marine environmental monitoring program in the future.
    Matched MeSH terms: Plastics
  3. Aralappanavar VK, Mukhopadhyay R, Yu Y, Liu J, Bhatnagar A, Praveena SM, et al.
    Sci Total Environ, 2024 May 10;924:171435.
    PMID: 38438042 DOI: 10.1016/j.scitotenv.2024.171435
    The harmful effects of microplastics (MPs) pollution in the soil ecosystem have drawn global attention in recent years. This paper critically reviews the effects of MPs on soil microbial diversity and functions in relation to nutrients and carbon cycling. Reports suggested that both plastisphere (MP-microbe consortium) and MP-contaminated soils had distinct and lower microbial diversity than that of non-contaminated soils. Alteration in soil physicochemical properties and microbial interactions within the plastisphere facilitated the enrichment of plastic-degrading microorganisms, including those involved in carbon (C) and nutrient cycling. MPs conferred a significant increase in the relative abundance of soil nitrogen (N)-fixing and phosphorus (P)-solubilizing bacteria, while decreased the abundance of soil nitrifiers and ammonia oxidisers. Depending on soil types, MPs increased bioavailable N and P contents and nitrous oxide emission in some instances. Furthermore, MPs regulated soil microbial functional activities owing to the combined toxicity of organic and inorganic contaminants derived from MPs and contaminants frequently encountered in the soil environment. However, a thorough understanding of the interactions among soil microorganisms, MPs and other contaminants still needs to develop. Since currently available reports are mostly based on short-term laboratory experiments, field investigations are needed to assess the long-term impact of MPs (at environmentally relevant concentration) on soil microorganisms and their functions under different soil types and agro-climatic conditions.
    Matched MeSH terms: Plastics*
  4. Auta HS, Abioye OP, Aransiola SA, Bala JD, Chukwuemeka VI, Hassan A, et al.
    J Environ Manage, 2022 Feb 15;304:114273.
    PMID: 34902688 DOI: 10.1016/j.jenvman.2021.114273
    In-situ bioremediation of mangrove soil contaminated with polyethylene terephthalate (PET) and polystyrene (PS) microplastics was investigated using indigenous microbial consortium with adequate capacity to degrade the plastics. Eight (8) bacteria were isolated from plastic/microplastic-inundated mangrove soil and screened for the ability to degrade PET and PS microplastics. Optical density at 600 nm and colony forming unit counts were measured to evaluate the growth response of the microbes in the presence of PS and PET microplastics at different times of exposure. Structural and surface changes that occurred post biodegradation on the microplastics were determined through EDS and SEM analysis. The obtained results demonstrated the elongation and disappearance of peaks, suggesting that the microbial consortium could modify both types of microplastics. The overall results of the microplastic degradation showed varied degrees of weight loss after 90 experimental days, with the treated plot recorded 18% weight loss. The augmented soil was increased in the concentrations of Si S, and Fe and decreased in the concentrations of C, O, Na, Mg, Al, Cl, and K after bioremediation.
    Matched MeSH terms: Plastics
  5. Auta HS, Emenike CU, Fauziah SH
    Environ Pollut, 2017 Dec;231(Pt 2):1552-1559.
    PMID: 28964604 DOI: 10.1016/j.envpol.2017.09.043
    The continuous accumulation of microplastics in the environment poses ecological threats and has been an increasing problem worldwide. In this study, eight bacterial strains were isolated from mangrove sediment in Peninsular Malaysia to mitigate the environmental impact of microplastics and develop a clean-up option. The bacterial isolates were screened for their potential to degrade UV-treated microplastics from polyethylene (PE), polyethylene terephthalate (PET), polypropylene (PP), and polystyrene (PS). Only two isolates, namely, Bacillus cereus and Bacillus gottheilii, grew on a synthetic medium containing different microplastic polymers as the sole carbon source. A shake flask experiment was carried out to further evaluate the biodegradability potential of the isolates. Degradation was monitored by recording the weight loss of microplastics and the growth pattern of the isolates in the mineral medium. The biodegradation extent was validated by assessment of the morphological and structural changes through scanning electron microscopy and Fourier transform infrared spectroscopy analyses. The calculated weight loss percentages of the microplastic particles by B. cereus after 40 days were 1.6%, 6.6%, and 7.4% for PE, PET, and PS, respectively. B. gottheilii recorded weight loss percentages of 6.2%, 3.0%, 3.6%, and 5.8% for PE, PET, PP, and PS, respectively. The designated isolates degraded the microplastic material and exhibited potential for remediation of microplastic-contaminated environment. Biodegradation tests must be conducted to characterize the varied responses of microbes toward pollutants, such as microplastics. Hence, a novel approach for biodegradation of microplastics must be developed to help mitigate the environmental impact of plastics and microplastic polymers.
    Matched MeSH terms: Plastics/analysis*
  6. Auta HS, Emenike CU, Fauziah SH
    Environ Int, 2017 May;102:165-176.
    PMID: 28284818 DOI: 10.1016/j.envint.2017.02.013
    The presence of microplastics in the marine environment poses a great threat to the entire ecosystem and has received much attention lately as the presence has greatly impacted oceans, lakes, seas, rivers, coastal areas and even the Polar Regions. Microplastics are found in most commonly utilized products (primary microplastics), or may originate from the fragmentation of larger plastic debris (secondary microplastics). The material enters the marine environment through terrestrial and land-based activities, especially via runoffs and is known to have great impact on marine organisms as studies have shown that large numbers of marine organisms have been affected by microplastics. Microplastic particles have been found distributed in large numbers in Africa, Asia, Southeast Asia, India, South Africa, North America, and in Europe. This review describes the sources and global distribution of microplastics in the environment, the fate and impact on marine biota, especially the food chain. Furthermore, the control measures discussed are those mapped out by both national and international environmental organizations for combating the impact from microplastics. Identifying the main sources of microplastic pollution in the environment and creating awareness through education at the public, private, and government sectors will go a long way in reducing the entry of microplastics into the environment. Also, knowing the associated behavioral mechanisms will enable better understanding of the impacts for the marine environment. However, a more promising and environmentally safe approach could be provided by exploiting the potentials of microorganisms, especially those of marine origin that can degrade microplastics.

    CAPSULE: The concentration, distribution sources and fate of microplastics in the global marine environment were discussed, so also was the impact of microplastics on a wide range of marine biota.

    Matched MeSH terms: Plastics/analysis*
  7. Azfaralariff A, Mat Lazim A, Amran NH, Mukhtar NH, Bakri ND, Azrihan NN, et al.
    Waste Manag Res, 2023 Jul;41(7):1219-1226.
    PMID: 36883418 DOI: 10.1177/0734242X231155395
    In recent years, the environmental pollution of microplastics (MPs) has increasingly drawn our attention. MPs are small fragments of plastics that are commonly dispersed in the environment. The accumulation of environmental MPs is due to population growth and urbanization, while natural disasters such as hurricanes, flooding and human activity may influence their distribution. The leaching of chemicals from MPs raises a significant safety problem and environmental approaches aimed at reducing the use and recycling of plastics, with the replacement by bioplastics and wastewater treatment developments are called for. This summary also helps in demonstrating the connection between terrestrial and freshwater MPs and wastewater treatment plants as the major contributors to environmental MPs by discharges of sludge and effluent. More research on the classification, detection, characterization and toxicity of MPs are essential to enable greater options and solutions. Control initiatives need to intensify the comprehensive study of MP waste control and management information programmes in the fields of institutional engagement, technological research and development, legislation and regulation. A comprehensive quantitative analysis approach for MPs should be created in the future, and more reliable traceability analysis methods should be built to examine further its environmental activity and existence, where this should be done to improve scientific research on MP pollution in terrestrial, freshwater and marine environments and hence, develop more scientific and rational control policies.
    Matched MeSH terms: Plastics/analysis; Plastics/toxicity; Plastics/chemistry
  8. Azman MA, Ramli MZ, Che Othman SF, Shafiee SA
    Mar Pollut Bull, 2021 Sep;170:112630.
    PMID: 34146861 DOI: 10.1016/j.marpolbul.2021.112630
    This study investigated the accumulation of debris at four sites, namely, Gebeng, Batu Hitam, Cherok Paloh, and Air Leleh, along the Pahang coastline, Peninsular Malaysia from March 2019 to February 2020. Plastic was the dominant debris (86.1%) and followed by cloth/fabric-based debris (6.0%), processed lumber debris (3.3%), rubber (2.7%), glass (1.5%), and metal (0.4%). The land-based debris (82.0%) was the major source of the deposition of marine waste. A statistically significant relationship was found between the seasonal variation and marine debris density in tidal and seasonal current along the Pahang coastline. In general, the Northeast Monsoon season had a higher amount of debris than the Southwest Monsoon season.
    Matched MeSH terms: Plastics
  9. Azura Azami N, Ira Aryani W, Aik-Hong T, Amirul AA
    Protein Expr Purif, 2019 03;155:35-42.
    PMID: 30352276 DOI: 10.1016/j.pep.2018.10.008
    Depolymerase is an enzyme that plays an important role in the hydrolysis of polyhydroxyalkanoates [PHAs]. In the current study, Burkholderia cepacia DP1 was obtained from Penang, Malaysia in which the enzyme was purified using ion exchange and gel filtration (Superdex-75) column chromatography. The molecular mass of the enzyme was estimated to be 53.3 kDa using SDS-PAGE. The enzyme activity was increased to 36.8 folds with the recovery of 16.3% after purification. The enzyme activity was detected between pH 6.0-10 and at 35-55 °C with pH 6.0 and 45 °C facilitating the maximum activity. Depolymerase was inactivated by Tween-20, Tween-80, SDS and PMSF, but insensitive to metal ions (Mg2+, Ca2+, K+, Na2+, Fe3+) and organic solvents (methanol, ethanol, and acetone). The apparent Km values of the purified P(3HB) depolymerase enzyme for P(3HB) and P(3HB-co-14%3HV) were 0.7 mg/ml and 0.8 mg/ml, respectively. The Vmax values of the purified enzyme were 10 mg/min and 8.89 mg/min for P(3HB) and P(3HB-co-14%3HV), respectively. The current study discovered a new extracellular poly(3-hydroxybutyrate) [P(3HB)] depolymerase enzyme from Burkholderia cepacia DP1 isolated and purified to homogeneity from the culture supernatant. To the best of our knowledge, this is the first report demonstrating the purification and biochemical characterization of P(3HB) depolymerase enzyme from genus Burkholderia.
    Matched MeSH terms: Plastics/chemistry
  10. Behera DP, Kolandhasamy P, Sigamani S, Devi LP, Ibrahim YS
    Mar Pollut Bull, 2021 Apr;165:112100.
    PMID: 33581571 DOI: 10.1016/j.marpolbul.2021.112100
    Marine debris is a global issue with adverse impacts on marine organisms, ecological processes, aesthetics, and economies of nations. Several studies have been conducted to quantify the plastic debris along Indian beaches. This baseline study describes the results of a survey conducted on the types of plastic litters and their quantification during January to March 2020 along Mandvi beach in Gujarat. A quadrate having 10 × 10 m size was used for sampling the plastic litter on the shoreline. A total of 10 quadrates along the shore was considered for quantification of the plastic materials based on their density, color, and weight. The plastic material observed includes gutkha pouches, food wrappers, and fragments, along with plastic straws, cutleries, and fragments of various dimensions and thickness. The major contributing factors for the debris abundance in Mandvi beach are land-based sources and recreational activities. The results suggest that similar long-term projects covering extensive areas should be undertaken for accurate quantification of available debris and their impacts on coastal habitats of Gujarat.
    Matched MeSH terms: Plastics
  11. Boey JY, Mohamad L, Khok YS, Tay GS, Baidurah S
    Polymers (Basel), 2021 May 12;13(10).
    PMID: 34065779 DOI: 10.3390/polym13101544
    Overconsumption of plastic goods and improper handling of petroleum-derived plastic waste have brought a plethora of negative impacts to the environment, ecosystem and human health due to its recalcitrance to degradation. These drawbacks become the main driving force behind finding biopolymers with the degradable properties. With the advancement in biopolymer research, polyhydroxyalkanoate (PHA) and poly(lacyic acid) (PLA) and its composites have been alluded to as a potential alternative to replace the petrochemical counterpart. This review highlights the current synthesis process and application of PHAs and PLA and its composites for food packaging materials and coatings. These biopolymers can be further ameliorated to enhance their applicability and are discussed by including the current commercially available packaging products. Factors influencing biodegradation are outlined in the latter part of this review. The main aim of this review article is to organize the scattered available information on various aspects of PHAs and PLA, and its composites for packaging application purposes. It is evident from a literature survey of about 140 recently published papers from the past 15 years that PLA and PHA show excellent physical properties as potential food packaging materials.
    Matched MeSH terms: Plastics
  12. Bostan N, Ilyas N, Akhtar N, Mehmood S, Saman RU, Sayyed RZ, et al.
    Environ Res, 2023 Oct 01;234:116523.
    PMID: 37422115 DOI: 10.1016/j.envres.2023.116523
    Plastic is now considered part and parcel of daily life due to its extensive usage. Microplastic (MP) pollution is becoming a growing worry and has been ranked as the second most critical scientific problem in the realm of ecology and the environment. Microplastics are smaller in size than the plastic and are more harmful to biotic and as well as abiotic environments. The toxicity of microplastic depends upon its shape and size and increases with an increase in its adsorption capacity and their toxicity. The reason behind their harmful nature is their small size and their large surface area-to-volume ratio. Microplastic can get inside fruits, vegetables, seeds, roots, culms, and leaves. Hence microplastic enters into the food chain. There are different entry points for microplastic to enter into the food chain. Such sources can include polluted food, beverages, spices, plastic toys, and household (packing, cooking, etc.). The concentration of microplastic in terrestrial environments is increasing day by day. Microplastic causes the destruction of soil structure; destroys soil microbiota, cause depletion of nutrients in the soil, and their absorption by plants decreases plant growth. Apart from other environmental problems caused by microplastic, human health is also badly affected by microplastic pollution present in the terrestrial environment. The presence of microplastics in the human body has been confirmed. Microplastic enters into the body of humans in different possible ways. According to their way of entering the body, microplastics cause different diseases in humans. MPs also cause negative effects on the human endocrine system. At the ecosystem level, the impacts of microplastic are interconnected and can disrupt ecological processes. Although recently different papers have been published on several aspects of the microplastic present in the terrestrial environment but there is no complete overview that focus on the interrelationship of MPs in plants, and soil and their effect on higher animals like a human. This review provides a completely detailed overview of existing knowledge about sources, occurrences, transport, and effects of microplastic on the food chain and soil quality and their ecotoxicological effects on plants and humans.
    Matched MeSH terms: Plastics/toxicity
  13. Bourtsalas ACT, Yepes IM, Tian Y
    J Environ Manage, 2023 Oct 15;344:118604.
    PMID: 37459814 DOI: 10.1016/j.jenvman.2023.118604
    This study analyzes the regional implications of China's 2017 import ban on plastic waste by examining U.S. census data. A statistically significant decrease in total U.S. plastic waste exports was found, dropping from about 1.4 million tons to 0.6 million tons in the post-ban period. California remained the top exporter, throughout both pre- and post-ban periods, while South Carolina exhibited the highest per capita exports. Malaysia emerged as the largest importer of U.S. plastic waste, followed by Vietnam, Indonesia, and Thailand. The ban also led to a change in the composition of the exported plastic waste. Ethylene polymers increased from 32.6% of total exports in the pre-ban period to 46.9% in the post-ban period. Other plastics (vinyl chloride polymers, styrene polymers, and for plastics not elsewhere specified or included) decreased from 67.4% of total exports in the pre-ban period to 53.1% in the post-ban period. Moreover, we found that exporting plastic waste has significant environmental and human health impacts. For example, the Global Warming Potential (GWP) decreased from 20 million tons CO2-eq in the scenario where 100% of plastics are exported, or 25 million tons exported from the U.S. since 2002, to -11.1 million tons CO2-eq in the scenario where 100% of plastics are treated domestically. Transportation exacerbates these impacts for exported waste scenarios, increasing to 5.4 million tons CO2-eq when plastics are exported by ship while decreasing to 0.9 million tons CO2-eq for domestic treatment. Although exporting plastic waste is initially cost-effective, our study highlights that investing in domestic waste management can yield significant long-term benefits, considering the environmental and public health impacts. Therefore, it is crucial to prioritize context-specific solutions to address the challenges of the evolving global plastic waste landscape.
    Matched MeSH terms: Plastics*
  14. Butt FI, Muhammad N, Hamid A, Moniruzzaman M, Sharif F
    Int J Biol Macromol, 2018 Dec;120(Pt A):1294-1305.
    PMID: 30189278 DOI: 10.1016/j.ijbiomac.2018.09.002
    PHAs (polyhydroxyalkanoates) have emerged as biodegradable plastics more strongly in the 20th century. A wide range of bacterial species along with fungi, plants, oilseed crops and carbon sources have been used extensively to synthesize PHA on large scales. Alteration of PHA monomers in their structures and composition has led to the development of biodegradable and biocompatible polymers with highly specific mechanical properties. This leads to the incorporation of PHA in numerous biomedical applications within the previous decade. PHAs have been fabricated in various forms to perform tissue engineering to repair liver, bone, cartilage, heart tissues, cardiovascular tissues, bone marrow, and to act as drug delivery system and nerve conduits. A large number of animal trials have been carried out to assess the biomedical properties of PHA monomers, which also confirms the high compatibility of PHA family for this field. This review summarizes the synthesis of PHA from different sources, and biosynthetic pathways and biomedical applications of biosynthesized polyhydroxyalkanoates.
    Matched MeSH terms: Biodegradable Plastics/chemical synthesis; Biodegradable Plastics/therapeutic use; Biodegradable Plastics/chemistry*
  15. Chang BP, Hazizan Md Akil, Ramdziah bt Md Nasir
    Sains Malaysiana, 2015;44:819-825.
    As of today, ultra-high molecular weight polyethylene (UHMWPE) is a thermoplastic material normally used as bearing
    components for human joint replacements. However, formation of wear debris from UHMWPE after certain service
    periods may cause adverse effects which remain as unresolved issues. In this study, mechanical and dry sliding wear
    properties of UHMWPE reinforced with different loading of talc particles were investigated. The wear test was carried
    out using Ducom TR-20 pin-on-disc tester at different pressure velocity (pv) factors under dry sliding conditions. The
    worn surfaces and transfer films of pure UHMWPE and talc/UHMWPE composites were observed under scanning electron
    microscope (SEM). The experimental results showed that the microhardness increased with the increase of talc loadings
    in UHMWPE. The 20 wt. % talc/UHMWPE composites showed a 17% increment in microhardness as compared with pure
    UHMWPE. The dry sliding wear behaviour of UHMWPE was also improved upon the reinforcement of talc. The wear rate
    of UHMWPE decreased after incorporation of talc particles. The coefficient of friction (COF) increased slightly under low
    pv conditions. At high pv conditions, the COF decreased in values with increasing talc loadings. The improvement in
    wear behaviour may be attributed to the increase in load-carrying capacity and surface hardness of the talc/UHMWPE
    composites. SEM micrographs on worn surfaces showed that plastic deformation and grooving wear were dominant for
    UHMWPE. The plastic deformation and grooving wear were reduced upon the reinforcement of talc particles. The talc/
    UHMWPE composites produced smoother and uniform transfer films as compared to pure UHMWPE.
    Matched MeSH terms: Plastics
  16. Chek MF, Hiroe A, Hakoshima T, Sudesh K, Taguchi S
    Appl Microbiol Biotechnol, 2019 Feb;103(3):1131-1141.
    PMID: 30511262 DOI: 10.1007/s00253-018-9538-8
    Polyhydroxyalkanoates (PHAs) are biopolymers synthesized by a wide range of bacteria, which serve as a promising candidate in replacing some conventional petrochemical-based plastics. PHA synthase (PhaC) is the key enzyme in the polymerization of PHA, and the crystal structures were successfully determined using the catalytic domain of PhaC from Cupriavidus necator (PhaCCn-CAT) and Chromobacterium sp. USM2 (PhaCCs-CAT). Here, we review the beneficial mutations discovered in PhaCs from a structural perspective. The structural comparison of the residues involved in beneficial mutation reveals that the residues are near to the catalytic triad, but not inside the catalytic pocket. For instance, Ala510 of PhaCCn is near catalytic His508 and may be involved in the open-close regulation, which presumably play an important role in substrate specificity and activity. In the class II PhaC1 from Pseudomonas sp. 61-3 (PhaC1Ps), Ser325 stabilizes the catalytic cysteine through hydrogen bonding. Another residue, Gln508 of PhaC1Ps is located in a conserved hydrophobic pocket which is next to the catalytic Asp and His. A class I, II-conserved Phe420 of PhaCCn is one of the residues involved in dimerization and its mutation to serine greatly reduced the lag phase. The current structural analysis shows that the Phe362 and Phe518 of PhaC from Aeromonas caviae (PhaCAc) are assisting the dimer formation and maintaining the integrity of the core beta-sheet, respectively. The structure-function relationship of PhaCs discussed in this review will serve as valuable reference for future protein engineering works to enhance the performance of PhaCs and to produce novel biopolymers.
    Matched MeSH terms: Plastics
  17. Chek MF, Kim SY, Mori T, Tan HT, Sudesh K, Hakoshima T
    iScience, 2020 May 22;23(5):101084.
    PMID: 32388399 DOI: 10.1016/j.isci.2020.101084
    Biodegradable polyester polyhydroxyalkanoate (PHA) is a promising bioplastic material for industrial use as a replacement for petroleum-based plastics. PHA synthase PhaC forms an active dimer to polymerize acyl moieties from the substrate acyl-coenzyme A (CoA) into PHA polymers. Here we present the crystal structure of the catalytic domain of PhaC from Chromobacterium sp. USM2, bound to CoA. The structure reveals an asymmetric dimer, in which one protomer adopts an open conformation bound to CoA, whereas the other adopts a closed conformation in a CoA-free form. The open conformation is stabilized by the asymmetric dimerization and enables PhaC to accommodate CoA and also to create the product egress path. The bound CoA molecule has its β-mercaptoethanolamine moiety extended into the active site with the terminal SH group close to active center Cys291, enabling formation of the reaction intermediate by acylation of Cys291.
    Matched MeSH terms: Plastics
  18. Chek MF, Kim SY, Mori T, Arsad H, Samian MR, Sudesh K, et al.
    Sci Rep, 2017 07 13;7(1):5312.
    PMID: 28706283 DOI: 10.1038/s41598-017-05509-4
    Polyhydroxyalkanoate (PHA) is a promising candidate for use as an alternative bioplastic to replace petroleum-based plastics. Our understanding of PHA synthase PhaC is poor due to the paucity of available three-dimensional structural information. Here we present a high-resolution crystal structure of the catalytic domain of PhaC from Chromobacterium sp. USM2, PhaC Cs -CAT. The structure shows that PhaC Cs -CAT forms an α/β hydrolase fold comprising α/β core and CAP subdomains. The active site containing Cys291, Asp447 and His477 is located at the bottom of the cavity, which is filled with water molecules and is covered by the partly disordered CAP subdomain. We designated our structure as the closed form, which is distinct from the recently reported catalytic domain from Cupriavidus necator (PhaC Cn -CAT). Structural comparison showed PhaC Cn -CAT adopting a partially open form maintaining a narrow substrate access channel to the active site, but no product egress. PhaC Cs -CAT forms a face-to-face dimer mediated by the CAP subdomains. This arrangement of the dimer is also distinct from that of the PhaC Cn -CAT dimer. These findings suggest that the CAP subdomain should undergo a conformational change during catalytic activity that involves rearrangement of the dimer to facilitate substrate entry and product formation and egress from the active site.
    Matched MeSH terms: Biodegradable Plastics/metabolism
  19. Chen HL, Gibbins CN, Selvam SB, Ting KN
    Environ Pollut, 2021 Nov 15;289:117895.
    PMID: 34364115 DOI: 10.1016/j.envpol.2021.117895
    Microplastic pollution is widely recognised as a global issue, posing risks to natural ecosystems and human health. The combination of rapid industrial and urban development and relatively limited environmental regulation in many tropical countries may increase the amount of microplastic entering rivers, but basic data on contamination levels are lacking. This is especially the case in tropical South East Asian countries. In this paper, the abundance, composition and spatio-temporal variation of microplastic in the Langat River, Malaysia, were assessed, and the relationship between microplastic concentration and river discharge was investigated. Water samples were collected over a 12-month period from 8 sampling sites on the Langat, extending from forested to heavily urbanised and industrial areas. All 508 water samples collected over this period contained microplastic; mean concentration across all sites and times was 4.39 particles/L but extended up to 90.00 particles/L in some urban tributaries. Most microplastics were secondary in origin, and dominated by fibres. Microplastic counts correlated directly with river discharge, and counts increased and decreased in response to changes in flow. A time-integrated assessment of the microplastic load conveyed by the Langat suggested that the river is typically (50 % of the time) delivering around 5 billion particles per day to the ocean. The positive correlation between the concentration of microplastics and suspended sediments in the Langat suggested that continuously logging turbidity sensors could be used to provide better estimates of microplastic loads and improve assessment of human and ecological health risks.
    Matched MeSH terms: Plastics
  20. Chen HL, Selvam SB, Ting KN, Gibbins CN
    Environ Monit Assess, 2023 Jan 18;195(2):307.
    PMID: 36652034 DOI: 10.1007/s10661-022-10856-5
    Recent increase in awareness of the extent of microplastic contamination in marine and freshwater systems has heightened concerns over the ecological and human health risks of this ubiquitous material. Assessing risks posed by microplastic in freshwater systems requires sampling to establish contamination levels, but standard sampling protocols have yet to be established. An important question is whether sampling and assessment should focus on microplastic concentrations in the water or the amount deposited on the bed. On three dates, five replicated water and bed sediment samples were collected from each of the eight sites along the upper reach of the Semenyih River, Malaysia. Microplastics were found in all 160 samples, with mean concentrations of 3.12 ± 2.49 particles/L in river water and 6027.39 ± 16,585.87 particles/m2 deposited on the surface of riverbed sediments. Fibres were the dominant type of microplastic in all samples, but fragments made up a greater proportion of the material on the bed than in the water. Within-site variability in microplastic abundance was high for both water and bed sediments, and very often greater than between-site variability. Patterns suggest that microplastic accumulation on the bed is spatially variable, and single samples are therefore inadequate for assessing bed contamination levels at a site. Sites with the highest mean concentrations in samples of water were not those with the highest concentrations on the bed, indicating that monitoring based only on water samples may not provide a good picture of either relative or absolute bed contamination levels, nor the risks posed to benthic organisms.
    Matched MeSH terms: Plastics
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