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Fulltext Glutathione (GSH) improves sperm quality and testicular morphology in streptozotocin-induced diabetic mice

Abdullah F, Khan Nor-Ashikin MN, Agarwal R, Kamsani YS, Abd Malek M, Bakar NS, et al.

Asian J Androl, 2021 1 22;23(3):281-287.
PMID: 33473013 DOI: 10.4103/aja.aja_81_20

Diabetes mellitus (DM) is known to cause reproductive impairment. In men, it has been linked to altered sperm quality and testicular damage. Oxidative stress (OS) plays a pivotal role in the development of DM complications. Glutathione (GSH) is a part of a nonenzymatic antioxidant defense system that protects lipid, protein, and nucleic acids from oxidative damage. However, the protective effects of exogenous GSH on the male reproductive system have not been comprehensively examined. This study determined the impact of GSH supplementation in ameliorating the adverse effect of type 1 DM on sperm quality and the seminiferous tubules of diabetic C57BL/6NTac mice. GSH at the doses of 15 mg kg-1 and 30 mg kg-1 was given intraperitoneally to mice weekly for 6 consecutive weeks. The mice were then weighed, euthanized, and had their reproductive organs excised. The diabetic (D Group) showed significant impairment of sperm quality and testicular histology compared with the nondiabetic (ND Group). Diameters of the seminiferous lumen in diabetic mice treated with 15 mg kg-1 GSH (DGSH15) were decreased compared with the D Group. Sperm motility was also significantly increased in the DGSH15 Group. Improvement in testicular morphology might be an early indication of the protective roles played by the exogenous GSH in protecting sperm quality from effects of untreated type 1 DM or diabetic complications. Further investigation using different doses and different routes of GSH is necessary to confirm this suggestion.

Matched MeSH terms: Dietary Supplements
Dietary astaxanthin augments disease resistance of Asian seabass, Lates calcarifer (Bloch, 1790), against Vibrio alginolyticus infection

Lim KC, Yusoff FM, Shariff M, Kamarudin MS

Fish Shellfish Immunol, 2021 Jul;114:90-101.
PMID: 33838221 DOI: 10.1016/j.fsi.2021.03.025

This investigation describes the impacts of dietary provisioning with astaxanthin on hemato-biochemistry, non-specific immunity, and disease resistance of the Asian seabass, Lates calcarifer, against the virulent Vibrio alginolyticus; with specific reference to dose-response associations and variations over different post-infection periods (0-, 7-, and 14-day). Triplicate groups of fish weighing 28 g, on average, were fed various diets (C, the control or astaxanthin-free; AXT50, 50 mg astaxanthin kg-1 diet; AXT100, 100 mg astaxanthin kg-1 diet; and AXT150, 150 mg astaxanthin kg-1 diet) for 90 days and subsequently challenged with V. alginolyticus at the end of the feeding period. Experimental infection unveiled that supplemented fish demonstrated significant improvements (P

Matched MeSH terms: Dietary Supplements
Fulltext The therapeutic potential of curcumin in alleviating N-diethylnitrosamine and iron nitrilotriacetate induced renal cell tumours in mice via inhibition of oxidative stress: Implications for cancer chemoprevention

Iqbal M, Shah MD, Vun-Sang S, Okazaki Y, Okada S

Biomed Pharmacother, 2021 Jul;139:111636.
PMID: 33957566 DOI: 10.1016/j.biopha.2021.111636

This study was designed to reveal the protective effects of dietary supplementation of curcumin against renal cell tumours and oxidative stress induced by renal carcinogen iron nitrilotriacetate (Fe-NTA) in ddY male mice. The results showed that mice treated with a renal carcinogen, Fe-NTA, a 35% renal cell tumour incidence was noticed, whereas renal cell tumour occurrence was elevated to 80% in Fe-NTA promoted and N-diethylnitrosamine (DEN)-initiated mice as compared with saline- treated mice. No incidence of tumours has been observed in DEN-initiated non-promoted mice. Diet complemented with 0.5% and 1.0% curcumin fed prior to, during and after treatment with Fe-NTA in DEN-initiated animals, tumour incidence was reduced dose-dependently to about 45% and 30% respectively. Immunohistochemical studies also revealed the increased formation of 4-hydroxy-2-nonenal (HNE)-modified protein adducts and 8-hydroxy-2'-deoxyguanosine (8-OHdG) in kidney tissue of mice treated with an intraperitoneal injection of Fe-NTA (6.0 mg Fe/kg body weight.). Furthermore, Fe-NTA treatment of mice also resulted in significant elevation of malondialdehyde (MDA), serum urea, and creatinine and decreases renal glutathione. However, the changes in most of these parameters were attenuated dose-dependently by prophylactic treatment of animals with 0.5% and 1% curcumin diet, this may be due to its antioxidative impact of curcumin. These results suggest that intake of curcumin is beneficial for the prevention of renal cell tumours and oxidative stress damage mediated by renal carcinogen, Fe-NTA.

Matched MeSH terms: Dietary Supplements
Texturized mung bean protein as a sustainable food source: techno-functionality, anti-nutrient properties, in vivo protein quality and toxicity

Brishti FH, Chay SY, Muhammad K, Rashedi Ismail-Fitry M, Zarei M, Saari N

Food Funct, 2020 Oct 21;11(10):8918-8930.
PMID: 32996964 DOI: 10.1039/d0fo01463j

Mung bean is gaining attention as a sustainable and economic source of plant protein. The current study evaluates the techno-functionality, anti-nutrient properties, in vivo protein quality and toxicity of texturized mung bean protein (TMBP) produced under optimized conditions. Our work successfully produces TMBP with improved techno-functionalities that are crucial for meat-based food applications, credited to retained juiciness and fat-binding ability. Alkaline extraction and extrusion significantly reduce trypsin inhibitor, phytic acid and tannin content in TMBP. An in vivo study using Sprague-Dawley rats reveals the good protein quality of TMBP, with a true protein digestibility of 99.26% resembling casein (99.36%, control protein), a net protein utilization of 63.99% and a biological value of 64.46%. The good protein quality, increased lean muscle mass along with reduced cholesterol and triglyceride secures TMBP's potential as a Protein meal replacer and dietary suplement. Non-toxicity of TMBP is confirmed by normal serum biochemical parameters and healthy organs, ascertaining the safety of alkaline extraction. The current study elucidates the production of TMBP with improved techno-functionalities (for meat-based food applications), reduced anti-nutritional factors and high quality (for weight-watchers and malnourished individuals).

Matched MeSH terms: Dietary Supplements
Fulltext Supplementation of postbiotic RI11 improves antioxidant enzyme activity, upregulated gut barrier genes, and reduced cytokine, acute phase protein, and heat shock protein 70 gene expression levels in heat-stressed broilers

Humam AM, Loh TC, Foo HL, Izuddin WI, Zulkifli I, Samsudin AA, et al.

Poult Sci, 2021 Mar;100(3):100908.
PMID: 33518339 DOI: 10.1016/j.psj.2020.12.011

The aim of this work was to evaluate the impacts of feeding different levels of postbiotic RI11 on antioxidant enzyme activity, physiological stress indicators, and cytokine and gut barrier gene expression in broilers under heat stress. A total of 252 male broilers Cobb 500 were allocated in cages in environmentally controlled chambers. All the broilers received the same basal diet from 1 to 21 d. On day 22, the broilers were weighed and grouped into 7 treatment groups and exhibited to cyclic high temperature at 36 ± 1°C for 3 h per day until the end of the experiment. From day 22 to 42, broilers were fed with one of the 7 following diets: negative control, basal diet (0.0% RI11) (NC group); positive control, NC diet + 0.02% (w/w) oxytetracycline (OTC group); antioxidant control, NC diet + 0.02% (w/w) ascorbic acid. The other 4 other groups were as follows: NC diet + 0.2% cell-free supernatant (postbiotic RI11) (v/w), NC diet + 0.4% cell-free supernatant (postbiotic RI11) (v/w), NC diet + 0.6% cell-free supernatant (postbiotic RI11) (v/w), and NC diet + 0.8% cell-free supernatant (postbiotic RI11) (v/w). Supplementation of different levels (0.4, 0.6, and 0.8%) of postbiotic RI11 increased plasma glutathione peroxidase, catalase, and glutathione enzyme activity. Postbiotic RI11 groups particularly at levels of 0.4 and 0.6% upregulated the mRNA expression of IL-10 and downregulated the IL-8, tumor necrosis factor alpha, heat shock protein 70, and alpha-1-acid glycoprotein levels compared with the NC and OTC groups. Feeding postbiotic RI11, particularly at the level of 0.6%, upregulated ileum zonula occludens-1 and mucin 2 mRNA expressions. However, no difference was observed in ileum claudin 1, ceruloplasmin, IL-6, IL-2, and interferon expression, but downregulation of occludin expression was observed as compared with the NC group. Supplementation of postbiotic RI11 at different levels quadratically increased plasma glutathione peroxidase, catalase and glutathione, IL-10, mucin 2, and zonula occludens-1 mRNA expression and reduced plasma IL-8, tumor necrosis factor alpha, alpha-1-acid glycoprotein, and heat shock protein 70 mRNA expression. The results suggested that postbiotics produced from Lactiplantibacillus plantarum RI11 especially at the level of 0.6% (v/w) could be used as an alternative to antibiotics and natural sources of antioxidants in poultry feeding.

Matched MeSH terms: Dietary Supplements
Fulltext Selective uptake of alpha-tocotrienol and improvement in oxidative status in rat brains following short- and long- term intake of tocotrienol rich fraction

Musalmah, M., Leow, K.S., Nursiati, M.T., Raja Najmi Hanis Raja, l., Fadly Syah, A., Renuka, S., et al.

Malays J Nutr, 2013;19(2):251-259.
MyJurnal

Introduction: Tocotrienol exerts neuroprotective effects resulting in an improved circulating oxidative status. However, accumulation of tocotrienol due to longterm intake may exert pro-oxidant effects. Thus the effects of short- and longterm supplementation of vitamin E tocotrienol rich fraction (TRF) on the parameters of oxidative status in rat brains were determined. Methods: Wistar rats aged 3 months were supplemented with TRF for 3 or 8 months. Control groups received equivolume of distilled water. Rats were sacrificed and brains
harvested, weighed and homogenised. Supernatants were analysed for catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) activities, vitamin E and protein carbonyl. Results: A significant decline in the level of total vitamin E and its isomers with increasing age were found. TRF supplementation increased the level of total vitamin E with alpha-tocotrienol (ATT) being the major isomer raised. Glutathione peroxidase activity was also
significantly increased in the long-term supplemented group compared to the short-term supplemented and control groups. The results also showed significantly higher superoxide dismutase activity (p

Matched MeSH terms: Dietary Supplements
Fulltext Effects of conjugated linoleic acid supplementation and exercise on body fat mass and blood lipid profiles among overweight Iranians

Loh, Su Peng, Abas Mohaghehgi, Hanieh Fouladi

Malays J Nutr, 2018;24(2):203-213.
MyJurnal

Introduction: Conjugated linoleic acid (CLA) has been studied for its fat mass
reduction effects. This study aimed to determine the effects of CLA supplementation
on body fat mass (BFM) and selected blood lipid profiles among overweight Iranian.

Methods: A total of 180 adults with BMI = 26-29 kg/m2 and BFM exceeding 21%
and 28% for men and women, respectively were recruited through voluntary
participation from weight management clinics in Tehran. They were assigned
randomly to three groups as follows: Group (1) (control group) receives weight loss
diet only; Group (2) receives weight loss diet +3 gr/day CLA supplement (mixture
of cis-9, trans-11 and trans-10, cis-12) twice a day and Group (3) weight loss diet
+3 gr/day CLA supplement as Group (2) twice a day + regular exercise (walking at
5.5-6 km/h for at least 160 minutes/week). The trial was conducted for 12 weeks.
Anthropometric measurements and blood lipid profiles were determined at weeks 0,
6 and 12.

Results: Both Group 2 and Group 3 showed a significant between-group
difference in reduction of BFM (1.3% and 2.6% respectively) compared to Group 1.
Group 2 supplementation showed increased free fatty acid (FFA) (0.44 mM to 0.55
mM) and decreased HDL-chol (47.5 mg/dL to 42.0 mg/dL) between weeks 0 and
12. These results were not observed for Group 3.

Conclusion: Combination of CLA
supplementation with exercise showed BFM reduction in overweight Iranian adults.
Further research is suggested to verify the findings of this study.

Matched MeSH terms: Dietary Supplements
Warfarin and food, herbal or dietary supplement interactions: A systematic review

Tan CSS, Lee SWH

Br J Clin Pharmacol, 2021 02;87(2):352-374.
PMID: 32478963 DOI: 10.1111/bcp.14404

AIMS: To present an updated overview on the safety of concurrent use of food, herbal or dietary supplement and warfarin.
METHODS: A systematic literature review was performed on 5 databases from inception up to 31 December 2019. These interactions were classified depending on the likelihood of interaction and supporting evidences.
RESULTS: A total of 149 articles describing 78 herbs, food or dietary supplements were reported to interact with warfarin. These reports described potentiation with 45 (57.7%) herbs, food or dietary supplements while 23 (29.5%) reported inhibition and 10 (12.8%) reported limited impact on warfarin pharmacokinetics and pharmacodynamics. Twenty unique herb and dietary supplements also reported to result in minor bleeding events, such as purpura and gum bleeding as well as major events such as intracranial bleeding that led to death.
CONCLUSION: While most food, herbs and supplements can be safely taken in moderation, healthcare professionals should be aware of the increased risk of bleeding when taking several food and herbs. These include Chinese wolfberry, chamomile tea, cannabis, cranberry, chitosan, green tea, Ginkgo biloba, ginger, spinach, St. John's Wort, sushi and smoking tobacco. Patients should be counselled to continue to seek advice from their healthcare professionals when starting any new herbs, food or supplement.

Matched MeSH terms: Dietary Supplements
Fulltext Safety assessment of a new developed fruit juice product - mixed fruit Juice in experimental rats

Hadijah, H., Norazlanshah, H., Muhammad, I., Roowi, S.

International Food Research Journal, 2015;22(6):2657-2663.
MyJurnal

The interest in dietary antioxidants which are mainly found in fruits, has prompted research in
the field of commercial high antioxidant juice for healthy purposes. Fruits also are rich with antioxidants that help in reducing of degenerative diseases such as cancer, arthritis, cardiovascular
disease and inflammation. Based on the health claims from the natural antioxidants, a new healthy juice called Mixed Fruit Juice (MFJ) has been developed by using three combinations of local fruits (soursop, mango and kasturi lime). In order to promote the commercial use of this product, the safety evaluation is needed to be carried out. The 28-days repeated toxicity test has been conducted in female and male rats for pre-clinical safety assessment prior to human study. There was no mortality observed when varying doses of the MFJ (5, 10 and 20%) administered to all rats. Hematological analysis showed no significant differences in most parameters examined. There were no significant changes observed in the liver and kidney functions tests of all treated-rats as compared to control normal rats. Furthermore, lipid profiles and blood glucose level were also within the normal range as noted in control rats. The present data demonstrate that the supplementation of MFJ did not produce adverse effects on the body system of experimental rats. This is the first documented report on the safety assessment of
MFJ in rats.

Matched MeSH terms: Dietary Supplements
Fulltext The Safety Of Capsules Containing Lactic Acid Bacilli

Paraidathathu, Thomas, Lee, S.H.

Ann Dent, 1999;6(1):-.
MyJurnal

The population in Malaysia use various types of health and food supplements. These products are considered safe and are used without any concern for their toxicity. Among the products used as health supplements are products that contain lactic acid bacteria. This project studied the acute and subacute toxicity of a product containing minerals, herbs, vitamins and live lactic acid bacteria, on Sprague- Dawley rats. Acute toxicity was tested 24 hours after a single dose and subacute toxicity was studied 24 hours after 7 days of daily dosing. The parameters that were studied were alanine aminotransferase (AL T,SGPT), aspartate aminotransferase (AST, SGOT), serum urea, ratios of weight of kidney and liver weight to body weight and percentage changes in body weights. The contents of capsules of the product (6, 8 or lO capsules for acute studies and 6, 10 and 12 for subacute studies) were mixed with corn oil and fed orally to rats. Control rats were fed with corn oil alone. In the acute studies, the level of ALT in the rats treated with the contents of the capsule was lower than controls. There were no significant changes in the other parameters of the rats in the treatment groups as compared to controls. There were no significant differences in all the parameters between rats in the treatment groups as compared to controls in the subacute studies. Sprague-Dawley rats fed with high doses of the product did not show signs of toxicity in the parameters that were studied.

Matched MeSH terms: Dietary Supplements
Fulltext Phenolics, flavonoids content and antioxidant activities of 4 Malaysian herbal plants

Azizah Othman, Nor Juwariah Mukhtar, Nurul Syakirin Ismail, Sui Kiat Chang

International Food Research Journal, 2014;21(2):759-766.
MyJurnal

Water and ethanolic extracts of four Malaysian local herbs, Tenggek burung (Melicope Iunu-ankenda), Kesum (Polygonum minus), Curry leave (Murraya Koenigii) and Salam (Eugenia polyantha) were investigated for their total phenolic content (TPC), total flavonoids content (TFC) and antioxidant activities (AA). Total phenolic content (TPC) of the herbs was determined using Folin-Ciocalteu reagent assay while the total flavonoid content (TFC) was determined based on aluminium chloride-flavonoid assay. The determination of AA was done using 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activitiy and β-carotene bleaching assays (BCB). Different extraction solvents significantly affected the TPC, TFC and AA of all herbs studied (p < 0.05). Both Tenggek burung and Kesum showed highest TPC, TFC and AA regardless of extraction solvents compared to Curry leave and Salam. All herbs showed strong positive correlation between TPC and DPPH assay. However, negative and low correlation between TFC and AA were obtained for all herbs studied. This showed that phenolic compounds of certain structures were responsible for the AA of all the herbs in this study. In conclusion, all herbs in this study except curry leave could be inexpensive sources of good natural antioxidants with nutraceutical potential in food industry.

Matched MeSH terms: Dietary Supplements
Fulltext Composition of tree peony (Paeonia suffruticosa) and Chinese apple flower (Malus spp.) buds

Voon, H.C., Bhat, Rajeev, Karim, A.A., Rosma, A.

International Food Research Journal, 2013;20(3):1173-1179.
MyJurnal

Traditionally, in Chinese medicine, tree peony and apple flower buds are used to prepare herbal decoctions to cure various ailments. As both of these flowers are popular and used regularly, providing scientific evidence on their basic composition is a necessity. Hence, in the present study, we report the chemical composition of these two flower buds. Results revealed tree peony and apple flower buds to have high crude protein (15.73 and 26.30%), fibre (13.11 and 16.51%), and carbohydrate (57.84 and 40.63%) contents. Both the flowers had significant amounts of essential amino acids and unsaturated fatty acids. Essential minerals present in tree peony and apple flowers were potassium (1540.37 and 1125.60 mg/100 g), calcium (462.46 and 449.98 mg/100 g), magnesium (241.51 and 164.23 mg/100 g), sodium (12.75 and 20.06 mg/100 g), and phosphorus (420.00 and 590.00 mg/100 g), respectively. Heavy metals (cadmium, nickel, mercury, lead, and arsenic) were detected in trace amounts (< 0.50 mg/100 g) in both the flower buds. Results obtained indicate that both flowers could be exploited as an additional source of nutraceutical for the development of new functional foods.

Matched MeSH terms: Dietary Supplements
Development of sustainable dye adsorption system using nutraceutical industrial fennel seed spent-studies using Congo red dye

Taqui SN, Yahya R, Hassan A, Nayak N, Syed AA

Int J Phytoremediation, 2017 Jul 03;19(7):686-694.
PMID: 28121459 DOI: 10.1080/15226514.2017.1284746

Fennel seed spent (FSS)-an inexpensive nutraceutical industrial spent has been used as an efficient biosorbent for the removal of Congo red (CR) from aqueous media. Results show that the conditions for maximum adsorption would be pH 2-4 and 30°C were ideal for maximum adsorption. Based on regression fitting of the data, it was determined that the Sips isotherm (R2 = 0.994, χ2 = 0.5) adequately described the mechanism of adsorption, suggesting that the adsorption occurs homogeneously with favorable interaction between layers with favorable interaction between layers. Thermodynamic analysis showed that the adsorption is favorable (negative values for ΔG°) and endothermic (ΔH° = 12-20 kJ mol-1) for initial dye concentrations of 25, 50, and 100 ppm. The low ΔH° value indicates that the adsorption is a physical process involving weak chemical interactions like hydrogen bonds and van der Waals interactions. The kinetics revealed that the adsorption process showed pseudo-second-order tendencies with the equal influence of intraparticle as well as film diffusion. The scanning electron microscopy images of FSS show a highly fibrous matrix with a hierarchical porous structure. The Fourier transform infrared spectroscopy analysis of the spent confirmed the presence of cellulosic and lignocellulosic matter, giving it both hydrophilic and hydrophobic properties. The investigations indicate that FSS is a cost-effective and efficient biosorbent for the remediation of toxic CR dye.

Matched MeSH terms: Dietary Supplements
Fulltext Dietary supplementation among doctors in Kelantan

Rohana Abdul Jalil, Zulkifli Ahmad

Malaysian Journal of Public Health Medicine, 2007;7(1):60-63.
MyJurnal

The aim of the study is to describe the usage of dietary supplemenu among doctors working in the hospitals in Kota Bharu, Kelantan. The method used was a postal questionaire survey of the doctors, both specialists and medical officers working in these hospitals. The results showed that
only 28.7% of the doctors are taking dietary supplements. Significantly more female doctors and doctors on long term medication, were taking these supplements. The dietary supplements commonly used were multivitamins and minerals (92.3%), garlic (26.9%), vitamin C (26.9%) and
lecithin (15.4%). The majority of the doctors who took dietary supplements (60%) felt their health status have improved. These supplements were mostly obtained from hospital pharmacies. Doctors who puchase their own supplements spend between RMIO to RMIZO per month. The main reasons given for taking these supplements were that the doctors jfelt healthier' and to ‘prevent or as a supplementary treatment’ for conditions like hypercholesterolaemia, hypertension, ischaemic heart disease and cancers. There were four doctors who regularly use traditional dietary supplements. Most doctors will generally advice their patients and children to take dietary supplements regularly, as they felt that it is important for health.

Matched MeSH terms: Dietary Supplements
Fulltext Complementary and alternative medicine use among breast cancer patients in a tertiary hospital in Malaysia

Raja, Lexshimi R.G., Oranye, N.O., Ho, S.E., Zuraida, J., Zulkifli, S.Z.

Malaysian Journal of Public Health Medicine, 2013;13(1):11-19.
MyJurnal

The Use of Complementary and alternative medicine (CAM) is a common phenomenon among women with breast cancer in Malaysia. This study examines the prevalence, types, influencing factors and source of information regarding CAM among women with breast cancer, using a cross sectional research design. A purposive sampling was used to recruit 103 women with breast cancer who were attending follow up care at the Radiotherapy and Oncology unit of Universiti Kebangsaan Malaysia Medical Centre. Data was gathered through the use of self–administered questionnaires. Ninety one women identified themselves as CAM users while twelve were non users. Among the CAM users, 57(62.6%) were Malays, 27(29.7%) Chinese and 7(7.7%) Indians. The common types of CAM used were vitamins, nutritional supplements and traditional medicines (TM). CAM was used more frequently upon diagnosis (63.7%) and after completion of conventional treatment (97.8%). With regard to decision-making, most of the women (62.6%) did not make the decision to use CAM and were mostly influenced by their husband and mother (67%). Mother (71.4%), husband (67%), and relatives (56%) were the main sources of information and all values showed significant association with CAM use (p

Matched MeSH terms: Dietary Supplements
Fulltext Establishing a culture system that supports in vitro expansion of adult microglia

Subramaniam, Hemavathy, Alireza Badiei, Ramasamy, Rajesh, Maha Abdullah, Vidyadaran, Sharmili

Malaysian Journal of Medicine and Health Sciences, 2010;6(2):25-30.
MyJurnal

Introduction: The vast majority of in vitro research on microglia are based on cells isolated from
neonatal animals (3-5 days of age). Studying microglia of adults has been limited by the lack of a suitable culture system that supports their growth. In this study, we describe a protocol for growing microglia of adults based on modifications of the technique for culturing microglia isolated from neonatal rats. Methods: Mixed glia isolated from adult rats (age range of 1 month to 3 years old) were seeded in
culture flasks coated with poly-L-lysine. Cells were maintained in DMEM media supplemented with
insulin-transferrin-selenium (ITS) and recombinant human macrophage colony-stimulating factor
(M-CSF). Mild trypsinisation was carried out to isolate microglia from mixed glia culture. Results:
Microglia cells of adult rats were successfully grown in vitro. For the expansion of adult microglia,
it was observed that coating the cell culture flasks with poly-L-lysine was crucial to encourage cell
adherence. The substitution of insulin in culture media with ITS was found to improve cell yield and
reduced the number of days required for culture from 28 days to 14 days. Addition of M-CSF to cell
culture medium, along with the improvisations described above provided the best adult microglia cell
yield (2.91 ± 0.56 x 106 cells) compared to the technique of replating cells (0.91 ± 0.65 x 106 cells;
p

Matched MeSH terms: Dietary Supplements
Fulltext Nutrients depictions of Barhi date palm (Phoenix dactylifera L.) kernels

Mahmud, I.A., Mirghani, M.E.S., Alkhatib, M.F.R., Yusof, F., Shahabuddin, M., Rashidi, O., et al.

International Food Research Journal, 2017;24(101):0-0.
MyJurnal

Phoenix dactylifera L (Date palm) is one of the oldest known fruit crops in the world, and
the consumption of date fruits is no longer restricted to the Middle Eastern countries. Date
palm kernels are waste products of date fruit industry which are normally being discarded.
Based on their dietary fiber content; date palm kernels (DPK) have been proposed to be used
as fiber-based food supplement, caffeine free coffee alternative and animal feed ingredient.
Hence, utilization of such waste is highly desirable for the date industry. To accommodate these
benefits, and subsequent to some uses associated with DPK, this study sought to investigate the
biochemical and nutritional values of the Barhi date palm kernels (BDPK) grown in Iraq. The
results show that BDPK is an excellent source of dietary fiber (66.24 g/100g). Glutamic acid
was found to be the predominant amino acid, (0.674 g/100g), followed by Arginine and aspartic
acid (0.437 g/100g and 0.320 g/100g, respectively). Potassium was the most occurring mineral
in BDPK (2.39 g/kg), and the main sugars were sucrose and fructose (0.548 g/100g and 0.249
g/100g, respectively). Gas-liquid chromatography revealed that the main unsaturated fatty acid
(USFA) was oleic acid (40.927 mg/100g), while the main saturated fatty acid (SFA) were lauric
acid (20.270 mg/100g) and myristic acid (12.288 mg/100g). Furthermore, the BDPK depicted
considerable concentrations of vitamins, in which vitamin B5 (40.4 mg/100g) showed the
highest value. The results obtained indicate a strong potential for BDPK to be used in human
nutrition, cosmetics, and pharmaceutical applications and may provide an important economic
advantage through increasing the utilization of BDKP while also additive value will be added
to the residue.

Matched MeSH terms: Dietary Supplements
Fulltext Fish gelatin nanoparticles and their food applications: A review

Akbar, I., Jaswir, I., Jamal, P., Octavianti, F.

International Food Research Journal, 2017;24(101):0-0.
MyJurnal

Considerable attention has been directed to nanoparticles based on gelatin biopolymer due to its numerous available active group sites for attaching target molecules and acting as a drug or nutraceutical delivery system aiming to improve the therapeutic effects and also to reduce the side effects of formulated drugs as gelatin is a natural biodegradable biocompatible polymer, nontoxic, readily available, cheap and is used in parental formulations. With mammalian gelatin (pig and cow) as the major source of gelatin production, alternatives are required due to sociocultural and health concerns to maintain halal status. This paper aims at reviewing fish skin gelatin from warm water species which can provide a potential alternative source of gelatin with almost the same rheological properties as mammalian gelatin and is a beneficial way to use fish waste such as skin, bones and fin which is generally discarded. The study also entails a lot of research being done in the field of nanoencapsulation of gelatin with various nutraceuticals as well as drug and gene therapy. There is an especially increasing interest in encapsulating biopeptides within gelatin nanoparticles in the functional food industry due to their role in preventing or delaying the onset of various diseases, food fortification, improvement of food quality, increase in shelf life, targeted peptide delivery and hence can be used as additives in food products. This review also attempts to provide an overview of the application of gelatin nanoparticles in nanoencapsulation in the food industry.

Matched MeSH terms: Dietary Supplements
Fulltext The effect of acute pomegranate extract supplementation on oxygen uptake in highly-trained cyclists during high-intensity exercise in a high altitude environment

Crum EM, Che Muhamed AM, Barnes M, Stannard SR

J Int Soc Sports Nutr, 2017;14:14.
PMID: 28572749 DOI: 10.1186/s12970-017-0172-0

BACKGROUND: Recent research has indicated that pomegranate extract (POMx) may improve performance during aerobic exercise by enhancing the matching of vascular oxygen (O2) provision to muscular requirements. POMx is rich in ellagitannin polyphenols and nitrates (NO3-), which are both associated with improvements in blood flow and O2 delivery. Primarily, this study aimed to determine whether POMx improves performance in a cycling time trial to exhaustion at 100%VO2max (TTE100%) in highly-trained cyclists. In addition, we investigated if the O2 cost (VO2) of submaximal exercise was lower with POMx, and whether any changes were greater at high altitude where O2 delivery is impaired.
METHODS: Eight cyclists exercised at three submaximal intensities before completing a TTE100% at sea-level (SEA) and at 1657 m of altitude (ALT), with pre-exercise consumption of 1000 mg of POMx or a placebo (PLAC) in a randomized, double-blind, crossover design. Data were analysed using a three way (treatment x altitude x intensity) or two-way (treatment x altitude) repeated measures ANOVA with a Fisher's LSD post-hoc analysis. Significance was set at p ≤ 0.05. The effect size of significant interactions was calculated using Cohen's d.
RESULTS: TTE100% performance was reduced in ALT but was not influenced by POMx (p > 0.05). Plasma NO3- were 10.3 μmol greater with POMx vs. PLAC (95% CI, 0.8, 19.7,F1,7 = 7.83, p 0.05). Submaximal VO2 values were not affected by POMx (p ≥ 0.05).
CONCLUSIONS: The restoration of SEA VO2 values at ALT is likely driven by the high polyphenol content of POMx, which is proposed to improve nitric oxide bioavailability. Despite an increase in VO2, no change in exercise performance occurred and therefore this study does not support the use of POMx as an ergogenic supplement.

Matched MeSH terms: Dietary Supplements
Fulltext Effects of naturally-produced lovastatin on feed digestibility, rumen fermentation, microbiota and methane emissions in goats over a 12-week treatment period

Candyrine SCL, Mahadzir MF, Garba S, Jahromi MF, Ebrahimi M, Goh YM, et al.

PLoS One, 2018;13(7):e0199840.
PMID: 29975711 DOI: 10.1371/journal.pone.0199840

Twenty male Saanen goats were randomly assigned to four levels of lovastatin supplementation and used to determine the optimal dosage and sustainability of naturally produced lovastatin from fermentation of palm kernel cake (PKC) with Aspergillus terreus on enteric methane (CH4) mitigation. The effects on ruminal microbiota, rumen fermentation, feed digestibility and health of animal were determined over three measuring periods (4-, 8- and 12-weeks) and the accumulation of lovastatin in tissues was determined at the end of the experiment. The diets contained 50% rice straw, 22.8% concentrates and 27.2% of various proportions of untreated or treated PKC to achieve the target daily intake level of 0 (Control), 2, 4 or 6 mg lovastatin/kg body weight (BW). Enteric CH4 emissions per dry matter intake (DMI), decreased significantly (P<0.05) and equivalent to 11% and 20.4%, respectively, for the 2 and 4 mg/kg BW groups as compared to the Control. No further decrease in CH4 emission thereafter with higher lovastatin supplementation. Lovastatin had no effect on feed digestibility and minor effect on rumen microbiota, and specifically did not reduce the populations of total methanogens and Methanobacteriales (responsible for CH4 production). Similarly, lovastatin had little effect on rumen fermentation characteristics except that the proportion of propionate increased, which led to a decreasing trend (P<0.08) in acetic: propionate ratio with increasing dosage of lovastatin. This suggests a shift in rumen fermentation pathway to favor propionate production which serves as H+ sink, partly explaining the observed CH4 reduction. No adverse physiological effects were noted in the animals except that treated PKC (containing lovastatin) was less palatable at the highest inclusion level. Lovastatin residues were detected in tissues of goats fed 6 mg lovastatin/kg BW at between 0.01 to 0.03 μg/g, which are very low.

Matched MeSH terms: Dietary Supplements

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