Displaying publications 541 - 560 of 1005 in total

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  1. Rojroongwasinkul N, Bao Kle N, Sandjaja S, Poh BK, Boonpraderm A, Huu CN, et al.
    Public Health Nutr, 2016 Jul;19(10):1741-50.
    PMID: 26592313 DOI: 10.1017/S1368980015003316
    OBJECTIVE: Health and nutritional information for many countries in the South-East Asian region is either lacking or no longer up to date. The present study aimed to calculate length/height percentile values for the South-East Asian Nutrition Survey (SEANUTS) populations aged 0·5-12 years, examine the appropriateness of pooling SEANUTS data for calculating common length/height percentile values for all SEANUTS countries and whether these values differ from the WHO growth references.

    DESIGN: Data on length/height-for-age percentile values were collected. The LMS method was used for calculating smoothened percentile values. Standardized site effects (SSE) were used for identifying large or unacceptable differences (i.e. $\mid\! \rm SSE \!\mid$ >0·5) between the pooled SEANUTS sample (including all countries) and the remaining pooled SEANUTS samples (including three countries) after weighting sample sizes and excluding one single country each time, as well as with WHO growth references.

    SETTING: Malaysia, Thailand, Vietnam and Indonesia.

    SUBJECTS: Data from 14202 eligible children were used.

    RESULTS: From pair-wise comparisons of percentile values between the pooled SEANUTS sample and the remaining pooled SEANUTS samples, the vast majority of differences were acceptable (i.e. $\mid\! \rm SSE \!\mid$ ≤0·5). In contrast, pair-wise comparisons of percentile values between the pooled SEANUTS sample and WHO revealed large differences.

    CONCLUSIONS: The current study calculated length/height percentile values for South East Asian children aged 0·5-12 years and supported the appropriateness of using pooled SEANUTS length/height percentile values for assessing children's growth instead of country-specific ones. Pooled SEANUTS percentile values were found to differ from the WHO growth references and therefore this should be kept in mind when using WHO growth curves to assess length/height in these populations.

    Matched MeSH terms: Thailand
  2. Koh MT, Liu CS, Chiu CH, Boonsawat W, Watanaveeradej V, Abdullah N, et al.
    Epidemiol Infect, 2016 Apr;144(6):1192-200.
    PMID: 26468043 DOI: 10.1017/S0950268815002393
    Surveillance data on the burden of pertussis in Asian adults are limited. This cross-sectional study evaluated the prevalence of serologically confirmed pertussis in adults with prolonged cough in Malaysia, Taiwan and Thailand. Adults (⩾19 years) with cough lasting for ⩾14 days without other known underlying cause were enrolled from outpatient clinics of seven public and/or private hospitals. Single blood samples for anti-pertussis toxin antibodies (anti-PT IgG) were analysed and economic impact and health-related quality of life (EQ-5D) questionnaires assessed. Sixteen (5·13%) of the 312 chronically coughing adults had serological evidence of pertussis infection within the previous 12 months (anti-PT IgG titre ⩾62·5 IU/ml). Three of them were teachers. Longer duration of cough, paroxysms (75% seroconfirmed, 48% non-seroconfirmed) and breathlessness/chest pain (63% seroconfirmed, 36% non-seroconfirmed) were associated with pertussis (P < 0·04). Of the seroconfirmed patients, the median total direct medical cost per pertussis episode in public hospitals (including physician consultations and/or emergency room visits) was US$13 in Malaysia, US$83 in Taiwan (n = 1) and US$26 in Thailand. The overall median EQ-5D index score of cases was 0·72 (range 0·42-1·00). Pertussis should be considered in the aetiology of adults with a prolonged or paroxysmal cough, and vaccination programmes considered.
    Study site in Malaysia: Klinik Kesihatan Seremban, Negeri Sembilan; Primary Care Clinic, University Malaya Medical Centre (UMMC), Kuala Lumpur, Malaysia
    Matched MeSH terms: Thailand
  3. Takaoka H, Srisuka W, Saeung A, Otsuka Y
    J Med Entomol, 2014 Nov 01;51(6):1109-15.
    PMID: 26309295 DOI: 10.1603/ME14039
    Simulium (Simulium) lomkaoense sp. nov. is described from females, males, pupae, and larvae in Thailand. This new species is assigned to the Simulium malyschevi Dorogostaisky, Rubtsov & Vlasenko species-group of the subgenus Simulium, and appears to be closely related to Simulium baimaii Kuvangkadilok & Takaoka from Thailand in having a similar shape of the female and male genitalia, pupal gill with two inflated filaments, and simple wall-pocket-shaped cocoon. This new species is compared taxonomically with S. baimaii and other related species. It represents the third species of the S. malyschevi species-group known from Thailand.
    Matched MeSH terms: Thailand
  4. How KY, Hong KW, Chan KG
    PeerJ, 2015;3:e1117.
    PMID: 26290785 DOI: 10.7717/peerj.1117
    Quorum sensing is a mechanism for regulating proteobacterial gene expression in response to changes in cell population. In proteobacteria, N-acyl homoserine lactone (AHL) appears to be the most widely used signalling molecules in mediating, among others, the production of extracellular virulence factors for survival. In this work, the genome of B. cepacia strain GG4, a plasmid-free strain capable of AHL synthesis was explored. In silico analysis of the 6.6 Mb complete genome revealed the presence of a LuxI homologue which correspond to Type I quorum sensing. Here, we report the molecular cloning and characterization of this LuxI homologue, designated as BurI. This 609 bp gene was cloned and overexpressed in Escherichia coli BL21(DE3). The purified protein was approximately 25 kDa and is highly similar to several autoinducer proteins of the LuxI family among Burkholderia species. To verify the AHL synthesis activity of this protein, high resolution liquid chromatography-mass spectrometry analysis revealed the production of 3-oxo-hexanoylhomoserine lactone, N-octanoylhomoserine lactone and 3-hydroxy-octanoylhomoserine lactone from induced E. coli BL21 harboring the recombinant BurI. Our data show, for the first time, the cloning and characterization of the LuxI homologue from B. cepacia strain GG4 and confirmation of its AHL synthesis activity.
    Matched MeSH terms: Thailand
  5. Amerena J, Chen SA, Sriratanasathavorn C, Cho JG, Dejia H, Omar R, et al.
    PMID: 26279634 DOI: 10.4137/CMC.S22022
    A prospective 1-year observational survey was designed to assess the management and control of atrial fibrillation (AF) in eight countries within the Asia-Pacific region. Patients (N = 2,604) with recently diagnosed AF or a history of AF ≤1 year were included. Clinicians chose the treatment strategy (rhythm or rate control) according to their standard practice and medical discretion. The primary endpoint was therapeutic success. At baseline, rhythm- and rate-control strategies were applied to 35.7% and 64.3% of patients, respectively. At 12 months, therapeutic success was 43.2% overall. Being assigned to rhythm-control strategy at baseline was associated with a higher therapeutic success (46.5% vs 41.4%; P = 0.0214) and a lower incidence of clinical outcomes (10.4% vs 17.1% P < 0.0001). Patients assigned to rate-control strategies at baseline had higher cardiovascular morbidities (history of heart failure or valvular heart disease). Cardiovascular outcomes may be less dependent on the choice of treatment strategy than cardiovascular comorbidities.
    Matched MeSH terms: Thailand
  6. Tan KY, van der Beek EM, Chan MY, Zhao X, Stevenson L
    Nutr Rev, 2015 Sep;73(9):634-41.
    PMID: 26269489 DOI: 10.1093/nutrit/nuv029
    The Association of Southeast Asian Nations aims to act as a single market and allow free movement of goods, services, and manpower. The purpose of this article is to present an overview of the current regulatory framework for health claims in Southeast Asia and to highlight the current barriers and opportunities in the regulatory frameworks in the Association of Southeast Asian Nations. To date, 5 countries in Southeast Asia, i.e., Indonesia, Malaysia, the Philippines, Singapore, and Thailand, have regulations and guidelines to permit the use of health claims on food products. There are inconsistencies in the regulations and the types of evidence required for health claim applications in these countries. A clear understanding of the regulatory frameworks in these countries may help to increase trade in this fast-growing region and to provide direction for the food industry and the regulatory community to develop and market food products with better nutritional quality tailored to the needs of Southeast Asian consumers.
    Matched MeSH terms: Thailand
  7. Lü L, Zhou HZ
    Zootaxa, 2015;3992(1):1-94.
    PMID: 26250256 DOI: 10.11646/zootaxa.3992.1.1
    Oxytelus species are widespread over all continents except Antarctica. Southeast Asia is one of the biodiversity hotspots in the world. In this paper, we review the Oxytelus species currently known from Southeast Asia. Seven species are described as new to science: O. castaneus sp. nov. (Vietnam), O. finitimus sp. nov. (Laos), O. grandiculus sp. nov. (Malaysia, Indonesia, and Thailand), O. insulanus sp. nov. (Malaysia and Indonesia), O. lompobatangensis sp. nov. and O. poecilopterus sp. nov. (Indonesia), and O. sublividus sp. nov. (Vietnam and Laos). Seven new synonymies are proposed: O. ruptus Fauvel = O. sublucidus Cameron, O. lucens Bernhauer = O. malaisei Scheerpeltz, O. puncticeps Kraatz = O. (Anotylus) micantoides Scheerpeltz, O. subferrugineus Cameron = O. kedirianus Cameron, O. megaceros Fauvel = O. kalisi Bernhauer, O. subincisus Cameron = O. fruhstorferi Cameron, O. antennalis Fauvel = O. cheesmani Bernhauer. Lectotypes are designated for the following names: O. armiger Fauvel, O. bellicosus Fauvel, O. discalis Cameron, O. gigantulus Fauvel, O. ginyuenensis Bernhauer, O. javanus Cameron, O. kalisi Bernhauer, O. kedirianus Cameron, O. lucens Bernhauer, O. lucidulus Cameron, O. mandibularis Cameron, O. megaceros Fauvel, O. nilgiriensis Cameron, O. subferrugineus Cameron, O. subincisus Cameron, O. sublucidus Cameron, O. subsculptus Cameron, O. antennalis Fauvel (New Caledonia, New Guinea and Australia), O. cheesmani Bernhauer (New Hebrides), O. cheesmanianus Cameron (Papua New Guinea), O. hingstoni Cameron (India), and O. tibetanus Bernhauer (China). The species O. (Anotylus) transversipennis Scheerpeltz is transferred to the genus Anotylus. Other than the new species, nine previously known species are redescribed, a key to 30 species and line drawings or color photographs of 42 species are provided. Thus, a total of 30 species are recorded from Southeast Asia.
    Matched MeSH terms: Thailand
  8. Tham KW, Abdul Ghani R, Cua SC, Deerochanawong C, Fojas M, Hocking S, et al.
    Obes Rev, 2023 Feb;24(2):e13520.
    PMID: 36453081 DOI: 10.1111/obr.13520
    Obesity is a chronic disease in which the abnormal or excessive accumulation of body fat leads to impaired health and increased risk of mortality and chronic health complications. Prevalence of obesity is rising rapidly in South and Southeast Asia, with potentially serious consequences for local economies, healthcare systems, and quality of life. Our group of obesity specialists from Bangladesh, Brunei Darussalam, India, Indonesia, Malaysia, Philippines, Singapore, Sri Lanka, Thailand, and Viet Nam undertook to develop consensus recommendations for management and care of adults and children with obesity in South and Southeast Asia. To this end, we identified and researched 12 clinical questions related to obesity. These questions address the optimal approaches for identifying and staging obesity, treatment (lifestyle, behavioral, pharmacologic, and surgical options) and maintenance of reduced weight, as well as issues related to weight stigma and patient engagement in the clinical setting. We achieved consensus on 42 clinical recommendations that address these questions. An algorithm describing obesity care is presented, keyed to the various consensus recommendations.
    Matched MeSH terms: Thailand
  9. Kuesap J, Suphakhonchuwong N, Eksonthi B, Huaihongthong S
    Malar J, 2025 Feb 27;24(1):63.
    PMID: 40016697 DOI: 10.1186/s12936-025-05305-w
    BACKGROUND: The genetic diversity of malaria parasites varies between regions in the world. The genetic polymorphisms of the genes Pvs48/45 and Pvs47 which encode gametocyte/gamete proteins of Plasmodium vivax, were studied because of their potential as transmission-blocking vaccine (TBV) targets. The aim of the present study was to investigate the genetic diversity of Pvs48/45 and Pvs47 in clinical isolates from endemic areas of Thailand.

    METHODS: Plasmodium vivax samples collected from four provinces neighbouring either Myanmar or Malaysia were analysed using polymerase chain reaction and nucleotide sequencing.   RESULTS: Fifteen and 18 amino acid substitutions were observed in 36 Pvs48/45 and 62 Pvs47 deduced amino acid sequences, respectively. Eleven haplotypes were identified in Pvs48/45 and 26 in Pvs47. Overall, low nucleotide diversities were observed for Pvs48/45 (π = 0.00104) and Pvs47 (π = 0.00321). Tajima's D, and Fu and Li's D* and F* values were negative for both genes, Pvs48/45 and Pvs47 while a significant difference was found in Pvs48/45 (P 

    Matched MeSH terms: Thailand
  10. Kim L, Maijan P, Yeo SF
    Sci Rep, 2025 Mar 17;15(1):9115.
    PMID: 40097660 DOI: 10.1038/s41598-025-93940-3
    A society risks experiencing inadequate educational services from higher education institutions if severe shortages of lecturers persist. Addressing such a critical social issue necessitates prioritizing lecturer satisfaction, as it is intrinsically linked to lecturer retention, reduced turnover rates, institutional cohesion, lecturer well-being, professional teaching development, and enhanced research and publication outcomes. The study aims to investigate the spillover effects of work-family conflict on job-related consequences, specifically stress and burnout, which subsequently shape work attitudes such as lecturer satisfaction, with occupational experience examined as a moderating variable. Data were collected from 450 Thai lecturers through a survey questionnaire, and a path analysis technique was employed for data analysis. The findings revealed that work-family conflict significantly influenced lecturer stress, which, in turn, was a key predictor of burnout. Additionally, burnout was found to significantly impact lecturer satisfaction, whereas stress did not directly influence satisfaction making burnout act as the mediator between stress and satisfaction. Furthermore, occupational experience moderated the relationship between burnout and lecturer satisfaction, highlighting its critical role in mitigating the adverse effects of burnout on overall work attitudes.
    Matched MeSH terms: Thailand
  11. Sittichaya W, Beaver RA, Smith SM
    Zootaxa, 2024 Apr 29;5446(1):65-76.
    PMID: 39645890 DOI: 10.11646/zootaxa.5446.1.3
    Four new species, Arixyleborus halabala Sittichaya, Beaver & Smith sp. nov., Arixyleborus longicauda Sittichaya, Beaver & Smith sp. nov., Arixyleborus vellus Sittichaya, Beaver & Smith sp. nov. are described from Thailand, and Arixyleborus liratus Sittichaya, Beaver & Smith sp. nov. from Thailand and East Malaysia (Sabah). With the inclusion of the species described here, the diversity of Arixyleborus is increased to 46 species, of which 20 occur in Thailand. A synoptic list with distributions and habitat types is provided.
    Matched MeSH terms: Thailand
  12. Pechdee P, Boonsuya A, Arunsan P, Thanchonnang C, La N, Rattanapitoon NK, et al.
    Trop Biomed, 2024 Dec 01;41(4):543-552.
    PMID: 39876513 DOI: 10.47665/tb.41.4.016
    Haplorchis taichui is the minute intestinal fluke (MIF), presents a significant public health concern in Thailand. Despite praziquantel (PZQ) being the main treatment, concerns over recurrent use and drug resistance have surfaced. Thus, local herbal alternatives effective against gastrointestinal parasites could be crucial for reducing issues, necessitating exploration of herbal sources for alternative treatments. The objective of this study is to evaluate the efficacy of crude extracts from Allium sativum, Thunbergia laurifolia, and Eurycoma longifolia against H. taichui newly excysted juveniles (NEJ). H. taichui NEJs were exposed to varying concentrations of A. sativum (5, 10, and 20 mg/ml), T. laurifolia, and E. longifolia (100, 200, and 400 mg/ml), alongside PZQ (20 mg/ml) and RPMI culture medium as controls. Motility assessment at different exposure times and morphological surface changes were conducted by scanning electron microscopy (SEM). Treatment with A. sativum, T. laurifolia, and E. longifolia inhibited motility in H. taichui NEJs, resulting in reduced relative motility (RM) values and survival index (SI). Significant differences were noted in A. sativum and E. longifolia treated groups, with T. laurifolia showing no significant differences compared to the negative control. Morphological damage, especially tegumental swelling, was evident across all treatment groups, notably severe in the A. sativum group. These findings suggest the potential effectiveness of crude extracts of A. sativum, T. laurifolia, and E. longifolia against H. taichui NEJs. However, further investigations are necessary to understand their mechanisms and key bioactive compounds for developing effective anti-parasitic agents against helminthic infections.
    Matched MeSH terms: Thailand
  13. López-Medina E, Di Pasquale A, Green A
    Hum Vaccin Immunother, 2026 Dec;22(1):2593730.
    PMID: 41586636 DOI: 10.1080/21645515.2025.2593730
    Healthcare professionals (HCPs) perform an essential role in facilitating dengue vaccine uptake, as they are trusted sources of vaccine information and their perspectives shape both patient acceptance and peer adoption. This study assessed HCPs' Knowledge, Attitudes, and Practices (KAP) regarding dengue vaccination, and identified factors influencing their likelihood to recommend the vaccine to patients ('recommendation') and to encourage colleagues to do the same ('advocacy'). A cross-sectional survey of 815 HCPs in Argentina, Brazil, Colombia, Indonesia, Malaysia, and Thailand was conducted. Globally, composite scores were lower for Knowledge (56%) than Attitudes (70%) and Practices (72%). In multivariate analysis, "Perceived ease of recommending dengue vaccines" (recommendation: regression coefficient 0.30, p 
    Matched MeSH terms: Thailand
  14. Siripatoomrut N, Romgaew T, Aobaom S
    Int J Legal Med, 2025 Sep;139(5):2109-2116.
    PMID: 40493061 DOI: 10.1007/s00414-025-03532-z
    This study evaluated the allele frequencies and forensic parameters of 23 autosomal short tandem repeat (aSTR) loci in 406 genotypes from a Cambodian population residing in Thailand. The loci were stored in the Thailand DNA database of the Police Forensic Science Center 1, Pathum Thani. No significant deviations from Hardy-Weinberg equilibrium were found for any loci after applying the Bonferroni correction (p > 0.002). SE33 exhibited the highest degree of genetic polymorphism, making it the most informative locus, while TPOX was the least informative. The combined power of discrimination (CPD) and combined power of exclusion (CPE) for the 23 aSTR loci were both at 0.9999. A phylogenetic tree was constructed to compare the Cambodian population with four other populations (Laotian, Thai, Vietnamese, and Malaysian). The results showed that the Cambodian population clustered with the other four populations, with the Laotian population being the most closely related. However, six dominant alleles unique to the Cambodian population in Thailand were identified, distinguishing this population from the Laotian, Thai, Vietnamese, and Malaysian populations. Moreover, two of these dominant alleles were exclusively found in the Cambodian population in Thailand and were not present in any of the 22 other compared populations. Therefore, the allele frequency data from this study can serve as a reference for identifying allele differences in other populations and can further enhance the accuracy of forensic human identification and kinship testing.
    Matched MeSH terms: Thailand
  15. Zhao J, Virani S, Sriplung H
    Health Policy Plan, 2017 Apr 01;32(3):430-436.
    PMID: 27935803 DOI: 10.1093/heapol/czw145
    Background: The national cervical cancer screening program in Thailand has been successful in reducing overall burden from this disease. However, evaluation on spatial and temporal scales is needed to assess the efficacy of this program in smaller regions. Here, we geographically assess incidence in a province with a uniquely heterogeneous distribution of lifestyle factors associated with religiosity.

    Methods: Cervical cancer cases were extracted from the provincial cancer registry from 1989 to 2013. Age-adjusted incidence rates were calculated using population statistics from the census bureau and adjusted to the Segi world standard population. Bayesian hierarchical modelling was employed to spatiotemporally map cervical cancer incidence trends in Songkhla province in 5-year period.

    Results: Overall, the incidence of cervical cancer decreased in Songkhla province. The three districts with a Muslim population of greater than 70% had consistently lower cervical cancer rates from 1989 to 2013 compared with the rest of the predominantly Buddhist districts. Hotspots of incidence were identified in Sadao, Hat Yai and the juncture of Mueang Songkhla and Singhanakhon in each 5-year period.

    Conclusions: Distinct cervical cancer incidence trends by religion over time indicate differences in sexual habits, lifestyle and religion-associated culture between Muslims and Buddhists, and suggest divergent risk factor profiles for these groups. The high incidence rates in Sadao and Hat Yai is likely explained by the main road to Malaysia, which runs across these two areas and has frequent commercial sex trade. Female sex workers should be targeted as a vulnerable population for screening efforts to address this continuing burden of cervical cancer.

    Matched MeSH terms: Thailand/epidemiology
  16. van Enter BJD, Lau YL, Ling CL, Watthanaworawit W, Sukthana Y, Lee WC, et al.
    Am J Trop Med Hyg, 2017 Jul;97(1):232-235.
    PMID: 28719309 DOI: 10.4269/ajtmh.16-0999
    Toxoplasma gondii primary infection in pregnancy is associated with poor obstetric outcomes. This study aimed to determine the seroprevalence of Toxoplasma infection in pregnant migrant and refugee women from Myanmar attending antenatal care in Thailand. A random selection of 199 residual blood samples from first antenatal screen in 2014-2015 was tested for Toxoplasma IgG and IgM antibodies. Seroprevalence of Toxoplasma infection was 31.7% (95% confidence interval = 25.6-38.4). Avidity testing in the three positive IgM cases indicated all were past infections. Multiparity (≥ 3 children) was significantly associated with higher Toxoplasma seropositivity rates. Seroprevalence of T. gondii infection in this pregnant population is similar to the only other report from Myanmar, where multiparity was also identified as a significant association. Toxoplasma infection is important in pregnant women. Nevertheless, in this marginalized population, this infection may be given less priority, due to resource constraints in providing the most basic components of safe motherhood programs.
    Matched MeSH terms: Thailand/epidemiology
  17. Wameadesa N, Sae-lim A, Hayeebilan F, Rattanachuay P, Sukhumungoon P
    PMID: 29642296
    Local Thai and imported Malaysian beef in southern Thailand area carry
    several Shiga toxin-producing Escherichia coli (STEC) serotypes. STEC O104 is an
    important pathogen capable of causing outbreaks with considerable morbidity
    and mortality. This study investigated the presence of E. coli O104 from local Thai
    and imported Malaysian beef obtained from markets in Hat Yai City, Songkhla
    Province during August 2015 - February 2016. Thirty-one E. coli O104 strains
    were isolated from 12 beef samples (16% and 23% Thai and imported Malaysian,
    respectively). Thirty strains possessed aggA (coding for a major component of
    AAF/I fimbriae), a gene associated with enteroaggregative E. coli (EAEC) pathotype,
    and all strains carried fimH (encoding Type 1 fimbriae). Thirty strains
    belonged to phylogenetic group B1 and one strain (from Malaysian beef) to group
    A. Agglutination of yeast cells was observed among 29 E. coli O104 strains. Investigation
    of stx2 phage occupancy loci demonstrated that sbcB was occupied in 12
    strains. Antimicrobial susceptibility assay revealed that 7 strains were resistant
    to at least one antimicrobial agent and two were multi-drug resistant. One strain
    carried extended spectrum β-lactamase gene blaCTX-M and three carried blaTEM. PFGE-generated DNA profiling showed identical DNA pattern between that of
    one EAEC O104 strain from Thai beef and another from Malaysian beef, indicating
    that these two strains originated from the same clone. This is the first report
    in Thailand describing the presence of EAEC O104 from both Thai and imported
    Malaysian beef and their transfer between both countries. Thorough surveillance
    of this pathogen in fresh meats and vegetables should help to prevent any possible
    outbreak of E. coli O104.
    Matched MeSH terms: Thailand/epidemiology
  18. Senapin S, Dong HT, Meemetta W, Gangnonngiw W, Sangsuriya P, Vanichviriyakit R, et al.
    J Fish Dis, 2019 Jan;42(1):119-127.
    PMID: 30397913 DOI: 10.1111/jfd.12915
    In Southeast Asia, a new disease called scale drop disease (SDD) caused by a novel Megalocytivirus (SDDV) has emerged in farmed Asian sea bass (Lates calcarifer) in Singapore, Malaysia and Indonesia. We received samples from an Eastern Thai province that also showed gross signs of SDD (loss of scales). Clinical samples of 0.2-1.1 kg L. calcarifer collected between 2016 and 2018 were examined for evidence of SDDV infection. Histopathology was similar to that in the first report of SDDV from Singapore including necrosis, inflammation and nuclear pyknosis and karyorrhexis in the multiple organs. Intracytoplasmic inclusion bodies were also observed in the muscle tissue. In a density-gradient fraction from muscle extracts, TEM revealed enveloped, hexagonal megalocytiviral-like particles (~100-180 nm). By PCR using primers derived from the Singaporean SDDV genome sequence, four different genes were amplified and sequenced from the Thai isolate revealing 98.7%-99.9% identity between the two isolates. Since viral inclusions were rarely observed, clinical signs and histopathology could not be used to easily distinguish between SDD caused by bacteria or SDDV. We therefore recommend that PCR screening be used to monitor broodstock, fry and grow-out fish to estimate the current impact of SDDV in Southeast Asia and to prevent its spread.
    Matched MeSH terms: Thailand/epidemiology
  19. Ninvilai P, Nonthabenjawan N, Limcharoen B, Tunterak W, Oraveerakul K, Banlunara W, et al.
    Transbound Emerg Dis, 2018 Oct;65(5):1208-1216.
    PMID: 29520997 DOI: 10.1111/tbed.12859
    Duck Tembusu virus (DTMUV), a newly emerging virus in ducks, was first reported in China in 2010. However, an unknown severe contagious disease associated with severe neurological signs and egg production losses in ducks, resembling to DTMUV infection, was observed in Thailand since 2007. To determine the presence of DTMUV in 2007, the clinical samples from affected ducks collected in 2007 were tested for DTMUV using pathological and virological analyses. Gross and histopathological lesions of affected ducks were mostly restricted to the ovary, brain and spinal cord, and correlated with the presence of flavivirus antigen in the brain and spinal cord samples. Subsequently, DTMUV was identified by RT-PCR and nucleotide sequencing of the polyprotein gene. Phylogenetic analysis of the polyprotein gene sequence revealed that the 2007 Thai DTMUV was a unique virus, belonged within DTMUV cluster 1, but distinctively separated from the Malaysian DTMUV, which was the most closely related DTMUV. It is interesting to note that the 2007 Thai DTMUV was genetically different from the currently circulating Thai and Chinese DTMUVs, which belonged to cluster 2. Our findings indicated that the 2007 Thai DTMUV emerged earlier from a common ancestor with the recently reported DTMUVs; however, it was genetically distinctive to any of the currently circulating DTMUVs. In conclusion, our data demonstrated the presence of DTMUV in the Thai ducks since 2007, prior to the first report of DTMUV in China in 2010. This study indicates that DTMUV may have circulated in the region long before 2010 and highlights high genetic diversity of DTMUVs in Asia.
    Matched MeSH terms: Thailand/epidemiology
  20. Hong SA, Peltzer K
    Subst Use Misuse, 2019;54(2):288-296.
    PMID: 30463459 DOI: 10.1080/10826084.2018.1517797
    BACKGROUND: Though alcohol and tobacco are the most commonly used substances among adolescents, little is known about the patterning of early adolescent substance use in Association of South-East Asian Nations (ASEAN) member states.

    OBJECTIVES: This study examined past month patterns of substances use and its gender difference among adolescents.

    METHODS: Cross-sectional samples among adolescents aged 13-16 years who completed the Global School-based Student Health Survey (GSHS) from eight ASEAN countries were included in the analysis (n = 40,212).

    RESULTS: Prevalence of past month any tobacco use was relatively high in Brunei, Indonesia, Malaysia, Thailand, and the Philippines (11-15%), but prevalence of dual cigarette and other forms of tobacco use was about 2-5% in the five countries. Past month alcohol consumption prevalence was also high in Thailand, Viet Nam, and the Philippines (16-24%), compared to the rest countries (1.4-8.2%). Moreover, prevalence of the concurrent alcohol and tobacco use was higher in Thailand and the Philippines (7 and 10%, respectively), particularly in boys (13 and 15%, respectively). Conclusions/importance: Almost 30-40% of the boys and 10-20% of girls in Malaysia, Philippines, Thailand, and Viet Nam are engaged in at least one of the two risk behaviors, and the concurrent alcohol and tobacco use was also relatively high among boys in those countries (5-15%). This study may provide some valuable insights on alcohol and tobacco policy in the region and requires to begin prevention and treatment programs in ASEAN member states.

    Matched MeSH terms: Thailand/epidemiology
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