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Fulltext Evaluation of extraction solvent on antioxidant activity, total phenolic and total flavonoid content in Elaeis Guineensis

Wan Zuraida Wan Mohd Zain, Siti Nur Lisha Mohd Ghazali, Samsiah Jusoh

ESTEEM Academic Journal, 2019;15(1):25-32.
MyJurnal

Oil palm or Elaeis guineens is a rich natural source of phenolic with flavonoid as the main constituents. These phenolics are potent antioxidants that can be used in the food industry, cosmetics and others. Therefore, the study was aimed to determine the effect of solvents which were methanol, ethyl acetate and hexane also different plant parts which were leaves, frond and fresh fruit bunch toward antioxidant activity (AOA), total phenolic content (TPC) and total flavonoid
content (TFC). The antioxidant was analysed using the DPPH method, TPC by Ciocalteu assay and TFC by aluminium chloride colorimetric assay. The result from ANOVA indicated that there was a difference (P < 0.05) in the extracting ability of each solvent and different plant parts for AOA, TPC and TFC. Generally, the result suggested that methanol give the highest antioxidant
activity, TPC and TFC compared to ethyl acetate and hexane. Therefore, the solvent used should be selected properly to allow for a high level of extraction efficiency.

Matched MeSH terms: Plant Leaves
Fulltext Separation of steviol glycosides from stevia rebaudiana using different aqueous extraction techniques

Mustapha bin Akil, Chong, Saw Peng, Norellia binti Bahari

Borneo International Journal of Biotechnology, 2020;1(1):77-87.
MyJurnal

Stevia rebaudiana has recently gained the attention of the food industry as one of the natural sweeteners. The sweet flavour is contributed by the glycoside compounds, especially the rebaudioside A and stevioside, which are the stevia main chemical markers. The aim of the work reported here was to compare the different extraction techniques of stevia leaves using different technologies such as the high pressure and ultrasonic on the extraction of steviol glycosides. In this paper, the extraction techniques yielding the highest glycosides from the leaves of Stevia rebaudiana were determined using hot water extraction (HWE), pressurised liquid extraction (PLE) and ultrasound-assisted extraction (UAE). The steviol glycoside yields were quantified by two chemical markers, rebaudioside A and stevioside of Stevia rebaudiana using highperformance liquid chromatography (HPLC) analysis. The result showed that the HWE managed to obtain 1,110 mg of steviol glycosides. The PLE obtained 294 mg steviol glycosides and the UAE obtained 427.5 mg steviol glycosides. As a conclusion, the results suggested the most efficient technique for stevia extraction in this study was the HWE.

Matched MeSH terms: Plant Leaves
Bacterial endosymbiont inhabiting Leucaena leucocephala leaves and their antioxidant and antidiabetic potential

Chigurupati S, Vijayabalan S, Selvarajan KK, Alhowail A, Kauser F

J Complement Integr Med, 2020 Dec 22;18(2):319-325.
PMID: 34187119 DOI: 10.1515/jcim-2020-0203

OBJECTIVES: Research on endosymbionts is emerging globally and is considered as a potential source of bioactive phytochemicals. The present study examines the antioxidant and antidiabetic of the endophytic crude extract isolated from Leucaena leucocephala leaves.
METHODS: Endophytic bacteria were isolated from the leaves of L. leucocephala and 16S rRNA gene sequencing was used to establish their identity. The in vitro antioxidant effect of endophytic crude extract (LL) was evaluated using 2-diphenyl-1-picrylhydrazyl (DPPH) and 2, 2'-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS) free radical scavenging methods. The in vitro antidiabetic properties of LL were evaluated using α-amylase and α-glucosidase enzyme inhibition assay.
RESULTS: The isolated endophytic bacteria were identified as Cronobacter sakazakii. LL displayed potent free radical scavenging effect against ABTS and DPPH radicals with an inhibitory concentration 50% (IC50) value of 17.49 ± 0.06 and 11.3 ± 0.1 μg/mL respectively. LL exhibited α-amylase and α-glucosidase inhibition with an IC50 value of 23.3 ± 0.08 and 23.4 ± 0.1 μg/mL respectively compared to the standard drug (acarbose). Both glucose loaded normoglycemic rats and STZ induced diabetic rats treated with LL (200 mg/kg) exhibited a considerable reduction in blood glucose levels p<0.01 after 8 h of treatment when compared to normal and diabetic control rats respectively.
CONCLUSIONS: Thus, the study shows that LL has a wellspring of natural source of antioxidants, and antidiabetic agents and phytoconstituents present in endophytes could be the rich source for bioactive compounds.

Matched MeSH terms: Plant Leaves
Fulltext Optimized aqueous extraction conditions for maximal phenolics, flavonoids and antioxidant capacity from Artocarpus heterophyllus (Jackfruit) leaves by response surface methodology (RSM)

Nor Hafiza Sayuti, ‘Ammar Akram Kamarudin, Nor Asma Ab. Razak, Norazalina Saad, Mohd Sabri Pak Dek, Norhaizan Mohd Esa

Malaysian Journal of Medicine and Health Sciences, 2020;16(2):135-144.
MyJurnal

Introduction: There are numerous studies on the therapeutic properties of Artocarpus heterophyllus. However, stud- ies on the aqueous extraction of A. heterophyllus leaves are limited. This present study was conducted to optimize the extraction conditions of A. heterophyllus leaves to yield the highest phenolic, flavonoids and antioxidant contents. Methods: Response surface methodology (RSM) was employed to obtain a higher phenolic extraction parameter(s) of A. heterophyllus leaves using Central Composite Design (CCD). The antioxidant activity was then determined via ABTS (2,29-azinobis (3 ethylbenzothiazoline-6-sulfonic acid)) and DPPH (2,2-Diphenyl-1-picrylhydrazyl) assay and analysis of the individual phenolics was performed by high performance liquid chromatography (HPLC). Results: The optimum extraction conditions with higher phenolics content and antioxidant activity was achieved at 81°C, 100 min and 40 mL/g sample with a good desirability value of 0.87. Under these optimized parameters, total phenolics and flavonoids were 174.48 ± 4.05 mg GAE/g sample and 21.44 ± 0.05 mg RE/g sample, respectively. Meanwhile, antioxidant activity via ABTS and DPPH assays were 90.88% ± 0.09 and 87.22% ± 0.62, respectively. Finally, under optimal extraction conditions revealed 4 compounds identified as chlorogenic acid, quercetin, rutin and kaempferol. Conclusion: The optimisation are promising to improve phenolic yield and antioxidant activity in A. heterophyllus leaves. It also proved that A. heterophyllus leaves can be used as an alternative natural antioxidant especially in medicinal applications since all identified compound possess significant biological activities for human health.

Matched MeSH terms: Plant Leaves
Fulltext Efficacy of the Aqueous Extract of Azadirachta indica Against the Marine Parasitic Leech and Its Phytochemical Profiling

Venmathi Maran BA, Josmeh D, Tan JK, Yong YS, Shah MD

Molecules, 2021 Mar 29;26(7).
PMID: 33805254 DOI: 10.3390/molecules26071908

Zeylanicobdella arugamensis (Hirudinea), a marine parasitic leech, not only resulted in the mortality of the host fish (Groupers) but also caused economic losses. The current study aimed to elucidate the antiparasitic efficacy of the aqueous extract of the Azadirachta indica leaves against Z. arugamensis and to profile the composition via LC-Q Exactive HF Orbitrap mass spectrometry. Different concentrations (25, 50 and 100 mg/mL) of A. indica extract were prepared and tested on the parasitic leeches. The total mortality of leeches was noticed with an exposure to the A. indica aqueous extract. The average times required for the aqueous extract at concentrations of 25, 50 and 100 mg/mL to kill the leeches were 42.65 ± 9.20, 11.69 ± 1.11 and 6.45 ± 0.45 min, respectively, in a dose-dependent manner. The Orbitrap mass spectrometry analysis indicated the presence of five flavonoids (myricetin 3-O-galactoside, trifolin, isorhamnetin, quercetin and kaempferol), four aromatics (4-methoxy benzaldehyde, scopoletin, indole-3-acrylic acid and 2,4-quinolinediol), three phenolics (p-coumaric acid, ferulic acid and phloretin) and two terpenoids (pulegone and caryophyllene oxide). Thus, our study indicates that A. indica aqueous extract is a good source of metabolites with the potential to act as a biocontrol agent against the marine parasitic leech in aquaculture.

Matched MeSH terms: Plant Leaves
Multiple resistance mechanisms associated with low pirimifos-methyl resistance in Culex pipiens pipiens in three populations of Tunisia

Tabbabi A, Daaboub J, Laamari A, Ben-Cheikh R, Feriani M, Boubaker C, et al.

Trop Biomed, 2018 Dec 01;35(4):1107-1114.
PMID: 33601857

The aim of this study was to evaluate the resistance status of Culex pipiens pipiens to pirimiphos-methyl insecticide. Three field populations of mosquitoes were collected from Tunisia and analyzed in laboratory. The samples studied showed low level of resistance not exceeding 5-folds. The low resistance recorded is particularly interesting, because it leaves a range of tools useable by vector control services. Both metabolic and target-site resistance mechanisms were identified. Different esterases of high activity including A2-B2, A4-B4 (and/or A5-B5) and B12 were observed in studied field samples using starch electrophoresis although opposite results were found using synergists tests on samples # 1 and 3. The polymorphism of AChE1 (Acetylcholinesterase) was analyzed and three phenotypes were detected: susceptible (ACHE1S, phenotype [SS]), resistant (ACHE1R, phenotype [RR]), and heterozygous (phenotype [RS]) of ACHE1. The resistance of Culex pipiens pipiens to pirimiphos-methyl remains low although the occurrences of multiple resistance mechanisms are able to confer high resistance levels to organophosphate insecticides. Therefore, continuous monitoring of resistance is fundamental for rational use of insecticides and mosquito control programs.

Matched MeSH terms: Plant Leaves
Fulltext Health benefits of Stevia rebaudiana Bertoni as zero calorie natural sweetener: a review

Haida, Z., Asikin, A., Hakiman, M.

International Food Research Journal, 2020;27(5):783-789.
MyJurnal

Stevia rebaudiana Bertoni is a perennial herbal species with a number of therapeutic properties. The leaves of S. rebaudiana are the most important part of the plant as they contain high
level of sweetener compounds known as steviol glycosides; which are known to possess
antioxidant, antimicrobial, and antifungal activities. Among the steviol glycosides, stevioside
and rebaudioside A are the most abundant sweetening compounds of interest, documented to
be 30 - 250 folds sweeter than sucrose or refined sugar. The therapeutic properties of the
species make it more interesting as they play important roles as anti-hyperglycaemic agent to
lower glucose levels in the blood, thus commonly used in the treatment of diabetes mellitus
and obesity. Known to have sweet tasting leaves, its extract which contains antioxidant
compounds have been used to treat cancer, reduce inflammatory, and hypertension. S. rebaudiana has drawn the attention of health-conscious fitness lovers all over the world as non-caloric sweetener, and has been widely cultivated for being the most suitable sweetening substitution.

Matched MeSH terms: Plant Leaves
Fulltext Morus alba L. Plant: Bioactive Compounds and Potential as a Functional Food Ingredient

Chen C, Mohamad Razali UH, Saikim FH, Mahyudin A, Mohd Noor NQI

Foods, 2021 Mar 23;10(3).
PMID: 33807100 DOI: 10.3390/foods10030689

Morus alba L. (M. alba) is a highly adaptable plant that is extensively incorporated in many traditional and Ayurveda medications. Various parts of the plant, such as leaves, fruits, and seeds, possess nutritional and medicinal value. M. alba has abundant phytochemicals, including phenolic acids, flavonoids, flavonols, anthocyanins, macronutrients, vitamins, minerals, and volatile aromatic compounds, indicating its excellent pharmacological abilities. M. alba also contains high nutraceutical values for protein, carbohydrates, fiber, organic acids, vitamins, and minerals, as well as a low lipid value. However, despite its excellent biological properties and nutritional value, M. alba has not been fully considered as a potential functional food ingredient. Therefore, this review reports on the nutrients and bioactive compounds available in M. alba leaves, fruit, and seeds; its nutraceutical properties, functional properties as an ingredient in foodstuffs, and a microencapsulation technique to enhance polyphenol stability. Finally, as scaling up to a bigger production plant is needed to accommodate industrial demand, the study and limitation on an M. alba upscaling process is reviewed.

Matched MeSH terms: Plant Leaves
Fulltext Shading Effects on Leaf Gas Exchange, Leaf Pigments and Secondary Metabolites of Polygonum minus Huds., an Aromatic Medicinal Herb

Mohd Yusof FF, Yaacob JS, Osman N, Ibrahim MH, Wan-Mohtar WAAQI, Berahim Z, et al.

Plants (Basel), 2021 Mar 23;10(3).
PMID: 33806923 DOI: 10.3390/plants10030608

The growing demand for high value aromatic herb Polygonum minus-based products have increased in recent years, for its antioxidant, anticancer, antimicrobial, and anti-inflammatory potentials. Although few reports have indicated the chemical profiles and antioxidative effects of Polygonum minus, no study has been conducted to assess the benefits of micro-environmental manipulation (different shading levels) on the growth, leaf gas exchange and secondary metabolites in Polygonum minus. Therefore, two shading levels (50%:T2 and 70%:T3) and one absolute control (0%:T1) were studied under eight weeks and 16 weeks of exposures on Polygonum minus after two weeks. It was found that P. minus under T2 obtained the highest photosynthesis rate (14.892 µmol CO2 m-2 s-1), followed by T3 = T1. The increase in photosynthesis rate was contributed by the enhancement of the leaf pigments content (chlorophyll a and chlorophyll b). This was shown by the positive significant correlations observed between photosynthesis rate with chlorophyll a (r2 = 0.536; p ≤ 0.05) and chlorophyll b (r2 = 0.540; p ≤ 0.05). As the shading levels and time interval increased, the production of total anthocyanin content (TAC) and antioxidant properties of Ferric Reducing Antioxidant Power (FRAP) and 2,2-Diphenyl-1-picrylhydrazyl (DPPH) also increased. The total phenolic content (TPC) and total flavonoid content (TFC) were also significantly enhanced under T2 and T3. The current study suggested that P.minus induce the production of more leaf pigments and secondary metabolites as their special adaptation mechanism under low light condition. Although the biomass was affected under low light, the purpose of conducting the study to boost the bioactive properties in Polygonum minus has been fulfilled by 50% shading under 16 weeks' exposure.

Matched MeSH terms: Plant Leaves
Fulltext Comparison of the effects of two laser photobiomodulation techniques on bio-physical properties of Zea mays L. seeds

Hasan M, Hanafiah MM, Alhilfy IHH, Aeyad Taha Z

PeerJ, 2021;9:e10614.
PMID: 33520446 DOI: 10.7717/peerj.10614

Background: Laser applications in agriculture have recently gained much interest due to improved plant characteristics following laser treatment before the sowing of seeds. In this study, maize seeds were exposed to different levels of laser treatment prior to sowing to improve their field performance. The aim of this study is to evaluate the impact of pre-sowing laser photobiomodulation on the field emergence and growth of treated maize seeds.
Methods: The maize seeds were first photobiomodulated with two lasers: 1) a helium-neon (He-Ne) red laser (632.8 nm), and 2) a neodymium-doped yttrium aluminum garnet (Nd:YAG) green laser (532 nm). Following three replications of randomized complete block design (RCBD), four irradiation treatments were applied (45 s, 65 s, 85 s, and 105 s) at two power intensities (2 mW/cm2 and 4 mW/cm2).
Results: Based on the results, maize seeds pretreated with a green laser and 2 mW/cm2 power intensity for 105 s exhibited the highest rate of seed emergence (96%) compared to the untreated control seeds with a lower seed emergence rate (62.5%). Furthermore, maize seeds treated with a red laser for 45 s showed an increased vigor index compared to the other treatment options and the control (P plant, and 3.4 cm in stem diameter compared to the untreated seeds. Moreover, the green laser treatment showed 57.4 days to anthesis, which was earlier than the untreated seeds (61.4 days). The results showed that the protein, oil, and starch contents of the seeds irradiated with the green laser were 17.54%, 6.18%, and 73.32%, respectively, compared to the seeds irradiated by the red laser with 16.51%, 6.33%, and 71.05%, respectively.
Conclusions: The photo biomodulation of maize seeds using a green laser light can improve the field emergence, seedling growth, and seed quality of the treated seed compared to the red laser treatment.

Matched MeSH terms: Plant Leaves
Fulltext Effect of plant ontogeny on quantity and chemical constituents of essential oil in sweet basil (Ocimum Basilicum L.) grown in Sudan

Aburigal, Yasmin A. A., Elmogtaba, Elfadl Y., Sirible, Awatif M., Hamza, Nada B., Hussein, Ismail H., Mirghani, Mohamed E. S.

Biological and Natural Resources Engineering Journal, 2020;3(2):1-6.
MyJurnal

Sweet basil (Ocimum basilicum L.) is a very important food additive as well as for its therapeutic and cosmetic potential. The composition of essential oils in plants is affected by genetics and environmental conditions, which is determined by growth region and harvesting time in terms of ontogenetical variability. This study was carried out to assess the effect of plant ontogeny (pre-flowering, at flowering, fruiting) on essential oil content and chemical constituents for four sweet basil cultivated under irrigation conditions in the experimental farm of the National Oilseed Processing Research Institute (NOPRI), University of Gezira, Sudan. The essential oils were hydro-distilled from the leaves using Clevenger apparatus and the chemical constituents were determined by GC-MS. The results reveal that the essential oil yield content ranged from 0.1% to 0.2% at pre-flowering stage, whereas the oil content obtained at post-flowering stage was 0.1% for the investigated accessions. The highest essential oil content was recorded at flowering stage (0.2-0.5%), where the two Sudanese accessions had the maximum content (0.5%). The major chemical constituents, linalool, citral, methyleugenol, and eucalyptol reported at different developmental stages, punctuated between 5.73% and 32.93% in the four investigated accessions.

Matched MeSH terms: Plant Leaves
Fulltext Development of Organic Insect-Repellent Biodegradable Pot

Nur Atirah Hasmi, Siti Sarah Azman, Nadia Nisha Musa, Wan Nurul Hidayah Wan Anuar, Nur Nadirah Rodzali

Jurnal Inovasi Malaysia, 2020;4(1):99-111.
MyJurnal

The potential of natural organic compounds as a repellent in biodegradable organic pot is budding into one of the new agriculture innovation. The use of recyclable materials in the production of the pot can reduce the excessive waste disposal problem and thus prevent pollution problems. The objectives are to recycle old newspapers into biodegradable pot for seedling of plants and to repel plant insect pests using organic compounds (Pandanus sp., Petroselinum crispum and Alium sativum). The production of the biodegradable pot incorporates the use of waste materials such as old newspapers and coconut husk together with natural organic materials such as tapioca flour, Pandanus amaryllifolius (pandan leaf), Petroselinum crispum (parsley) and Alium sativum (garlic). This product is distinguishable among others as it is environmental-friendly since the pot can naturally break over time (biodegradable). The aroma from the Pandanus amaryllifolius, Petroselinum crispum and Alium sativum can repel flying and crawling insect that may attack the crops. The organic insect-repellent biodegradable pot has a potential in replacing plastic polybags since the crops planted in the pot can be directly replanted in the soil without the need of disposing the pot. Root is allowed to grow through the pot where later on, it will be degraded. Apart from that, this pot has high commercial values since it is easy to be handled, low in production cost, eco-friendly where only recyclable and natural organic materials were used, and the most crucial point is it can act as an insect repellent.

Matched MeSH terms: Plant Leaves
Fulltext PHYTOTOXIC ACTIVITY OF GERANIOL-TREATED LEMONGRASS LEAF MULCH AGAINST COMMON WEEDS IN NURSERIES

NORLIYANA FATIN OMAR, CHUAH TSE SENG

UMT Journal of Undergraduate Research, 2019;1(1):1-11.
MyJurnal

Hand weeding continues to be one of the most laborious aspects of nursery
maintenance. Oxygenated monoterpene, an important group of secondary metabolite found in
essential oils, has a potential herbicidal activity that could be exploited as natural herbicide
whereas organic mulch could delay weed emergence. Thus, this study aimed to examine the
phytotoxicity of geraniol, an oxygenated monoterpene compound, in combination with
lemongrass leaf mulch against three common weeds, Eleusine indica (grass), Cyperus distans
(sedge), and Tridax procumbens (broadleaf). Greenhouse experiments were carried out by
treating 4.0 t/ha lemongrass leaf mulch with 7.5% (v/v) geraniol compound. The pretreated
mulch acted synergistically and inhibited the emergence and shoot growth of T. procumbens
completely. However, the pretreated mulch exhibited a moderate inhibitory effect on C. distans
emergence and growth. Geraniol-treated lemongrass leaf mulch acted synergistically and
inhibited the emergence of E. indica by 72%, but it acted antagonistically and caused a 45%
reduction of shoot biomass. The present findings suggest that geraniol-treated lemongrass leaf
mulch has potent herbicidal activity but its phytotoxic effect is species-dependent.

Matched MeSH terms: Plant Leaves
Fulltext Green chemistry synthesis of nanostructured zinc oxide powder using azadirachta indica extract for latent fingermark development

Revathi Rajan, Yusmazura Zakaria, Shaharum Shamsuddin, Nik Fakhuruddin Nik Hassan

Malaysian Journal of Medicine and Health Sciences, 2020;16(101):38-42.
MyJurnal

Introduction: Application of nano-engineered fingerprint dusting powders has been a recent trend to achieve latent fingermark development with superior ridge clarity. As such, efforts have been made to utilise natural resources to increase the sustainability of these emerging nano-engineered powders. Lithium-doped zinc oxide, primarily used as white pigments, have been previously applied to latent fingermarks with success. In the current study, nanostruc- tured zinc oxide, synthesised using neem extract as the reducing agent, was evaluated for fingermark development on non-porous surfaces. Methods: The reduction of zinc nitrate hexahydrate was facilitated by neem extract, pre- pared by boiling neem leaves in distilled water. The thick yellow paste recovered was calcined in the furnace to produce a light yellow powder. Physicochemical composition of the powder was determined using microscopic and spectroscopic instruments. The effectiveness of the powder was tested on natural fingermark deposited on several non-porous surfaces. Results: Nanostructured zinc oxide with particle size ranging in between 1 to 3 µm consisting of highly aggregated spherical particle with less than 100 nm dimensions were synthesised. Developed fingermarks revealed excellent ridge details and contrast on dark coloured surfaces. Studying the fingermark closely under scan- ning electron microscope displayed selective distribution of particle on the ridges of the fingermark residue and very minimal deposition on the fingermark valleys. Conclusion: Nanostructured zinc oxide fabricated using green chem- istry approach can be applied for the development of fingermark. Nevertheless, future works can be undertaken to enhance particle dispersity and to confer strong photoluminescence to the zinc oxide nanoparticles.

Matched MeSH terms: Plant Leaves
Fulltext Integrated Approach for Species Identification and Quality Analysis for Labisia pumila Using DNA Barcoding and HPLC

Tarmizi AAA, Wagiran A, Mohd Salleh F, Chua LS, Abdullah FI, Hasham R, et al.

Plants (Basel), 2021 Apr 07;10(4).
PMID: 33917172 DOI: 10.3390/plants10040717

Labisia pumila is a precious herb in Southeast Asia that is traditionally used as a health supplement and has been extensively commercialized due to its claimed therapeutic properties in boosting a healthy female reproductive system. Indigenous people used these plants by boiling the leaves; however, in recent years it has been marketed as powdered or capsuled products. Accordingly, accuracy in determination of the authenticity of these modern herbal products has faced great challenges. Lack of authenticity is a public health risk because incorrectly used herbal species can cause adverse effects. Hence, any measures that may aid product authentication would be beneficial. Given the widespread use of Labisia herbal products, the current study focuses on authenticity testing via an integral approach of DNA barcoding and qualitative analysis using HPLC. This study successfully generated DNA reference barcodes (ITS2 and rbcL) for L. pumila var. alata and pumila. The DNA barcode that was generated was then used to identify species of Labisia pumila in herbal medicinal products, while HPLC was utilized to determine their quality. The findings through the synergistic approach (DNA barcode and HPLC) implemented in this study indicate the importance of both methods in providing the strong evidence required for the identification of true species and to examine the authenticity of such herbal medicinal products.

Matched MeSH terms: Plant Leaves
Fulltext Novel Mangosteen-Leaves-Based Marker Ink: Color Lightness, Viscosity, Optimized Composition, and Microstructural Analysis

Mohd Basri MS, Liew Min Ren B, A Talib R, Zakaria R, Kamarudin SH

Polymers (Basel), 2021 May 14;13(10).
PMID: 34069259 DOI: 10.3390/polym13101581

Dry mangosteen leaves are one of the raw materials used to produce marker ink. However, research using this free and abundant resource is rather limited. The less efficient one-factor-at-a-time (OFAT) approach was mostly used in past studies on plant-based marker ink. The use of statistical analysis and the regression coefficient model (mathematical model) was considered essential in predicting the best combination of factors in formulating mangosteen leaf-based marker ink. Ideally, ink should have maximum color lightness, minimum viscosity, and fast-drying speed. The objective of this study to study the effect of glycerol and carboxymethyl cellulose (CMC) on the color lightness and viscosity of mangosteen-leaves-based marker ink. The viscosity, color lightness, and drying properties of the ink were tested, the significant effect of glycerol and CMC (responses) on ink properties was identified and the prediction model on the optimum value of the responses was developed by using response surface methodology (RSM). The microstructure of mangosteen leaves was analyzed to study the surface morphology and cell structure during dye extraction. A low amount of glycerol used was found to increase the value of color lightness. A decrease in CMC amounts resulted in low viscosity of marker ink. The optimum formulation for the ink can be achieved when the weight percents of glycerol, benzalkonium chloride, ferrous sulphate, and CMC are set at 5, 5, 1, and 3, respectively. SEM micrographs showed the greatest amount of cell wall structure collapse on samples boiled with the lowest amount of glycerol.

Matched MeSH terms: Plant Leaves
Fulltext Manilkara zapota (L.) P. Royen: potential source of natural antioxidants

Norain Mohd Tamsir, Norhaizan Mohd Esa, Siti Nursalwah Che Omar, Nurul Husna Shafie

Malaysian Journal of Medicine and Health Sciences, 2020;16(106):196-204.
MyJurnal

Introduction: Manilkara zapota (L.) P. Royen or sapodilla is a fruit-bearing tree that has been cultivated mainly in tropical areas including Mexico and South East Asia. The fruits and the other parts of M. zapota plant have been used since ages ago for various medicinal purposes. However, the data on the antioxidant properties of various parts of M. zapota is limited. Therefore, we aimed to measure the content and capacity of antioxidants in various M. zapota plant parts and also to screen the phytoconstituents present in the part with the highest antioxidant content and capacity. Methods: The in vitro antioxidant evaluation including the content of total phenolic (TPC) and total flavonoids (TFC) as well as β-carotene bleaching and 1-diphenyl-2-picrylhydrazyl (DPPH) scavenging ability of the leaves, seeds, flesh, and peels of M. zapota extracted by aqueous and ethanol were determined. The plant part that exhibited the highest TPC, TFC, and antioxidant capacity was selected for phytoconstituents identification using liq- uid chromatography mass spectrometry. Results: M. zapota leaves aqueous extract exhibited the highest TPC, TFC, and antioxidant capacities and therefore selected for phytoconstituents identification. Our study provide additional data in which a total of 39 phytoconstituents have been identified in the M. zapota leaves including m-coumaric acid, quinic acid, robinetinidol-4alpha-ol, isoorientin 6’’-O-caffeate, apocynin A, and C16 Sphinganine. Conclusion: Thus, our study revealed that M. zapota leaves aqueous extract has potential as a promising naturally-occurring an- tioxidant candidate which could be useful for medicinal and nutritional functions.

Matched MeSH terms: Plant Leaves
Fulltext Processing and Characterisation of Banana Leaf Fibre Reinforced Thermoplastic Cassava Starch Composites

Jumaidin R, Diah NA, Ilyas RA, Alamjuri RH, Yusof FAM

Polymers (Basel), 2021 Apr 28;13(9).
PMID: 33924842 DOI: 10.3390/polym13091420

Increasing environmental concerns have led to greater attention to the development of biodegradable materials. The aim of this paper is to investigate the effect of banana leaf fibre (BLF) on the thermal and mechanical properties of thermoplastic cassava starch (TPCS). The biocomposites were prepared by incorporating 10 to 50 wt.% BLF into the TPCS matrix. The samples were characterised for their thermal and mechanical properties. The results showed that there were significant increments in the tensile and flexural properties of the materials, with the highest strength and modulus values obtained at 40 wt.% BLF content. Thermogravimetric analysis showed that the addition of BLF had increased the thermal stability of the material, indicated by higher-onset decomposition temperature and ash content. Morphological studies through scanning electron microscopy (SEM) exhibited a homogenous distribution of fibres and matrix with good adhesion, which is crucial in improving the mechanical properties of biocomposites. This was also attributed to the strong interaction of intermolecular hydrogen bonds between TPCS and fibre, proven by the FT-IR test that observed the presence of O-H bonding in the biocomposite.

Matched MeSH terms: Plant Leaves
PHOTOSYNTHETIC AND RESPIRATORY CHARACTERISTICS OF MALAYAN SUN AND SHADE FERNS

Nasrulhaq-Boyce A, Mohamed MAH

New Phytol, 1987 Jan;105(1):81-88.
PMID: 33874033 DOI: 10.1111/j.1469-8137.1987.tb00112.x

A comparative study of four Malayan ferns, Christensenia aesculifolia (Bl.) Maxon, Tectaria singaporeana (Wall.) Ching, Abacopteris multilineata (Wall.) Ching and Hymenophyllum polyanthos Sw. from shady habitats and another four, Dicranopteris linearis (Burm.) Und., Lygodium scandens (L.) Sw., Blechnum orientate Linn, and Stenochlaena palustris (Burm.) Bedd. from sunlit habitats showed that the total chlorophyll content expressed on a gram fresh weight basis was greater in the shade ferns. There was little difference in the chlorophyll content between the sun and shade ferns when it was expressed on a per unit leaf area basis. The protein and protohaem content was greater in the sun ferns. Measurements of the in vitro photochemical activities of the photosystems I and II in isolated chloroplasts by means of an oxygen electrode showed higher rates in the sun ferns. As determined by spectrophotometric analysis, the photosynthetic cytochrome content from isolated chloroplasts was greater in the sun ferns. The results indicate that the sun ferns have physiological characteristics favouring greater capacity for photosynthesis. Mitochondria isolated from the sun ferns showed faster rates of electron transport using exogenous NADH as substrate.

Matched MeSH terms: Plant Leaves
Fulltext Brief Dataset on produced handsheet from oil palm residue lignocellulose treated with Bacillus cereus on mechanical and physical characterization

Syarifah SM, Mohd Kassim AS, Mohd Aripin A, Chan CM, Zainulabidin MH, Ishak N, et al.

Data Brief, 2021 Jun;36:107030.
PMID: 34026964 DOI: 10.1016/j.dib.2021.107030

This article presents experimental data on oil palm biomass (oil palm leaves, oil palm trunk and empty fruit bunch) handsheet production characterization by biodelignification treatment using Bacillus cereus extracted from termite gut (Coptotermus curvignathus). It associates the lignocellulose chemical composition obtained via technical association pulp and paper industry TAPPI T 222 om-02 testing on lignin content reduction determination, holocellulose and hemicellulose content determination (Kurscher-Hoffner method). Several data obtained for handsheet characterization presents brightness, opacity, contrast ratio, din transparency, thickness, bursting and tearing indexes are collected. Handsheet surface morphology was also observed on ratio of gaps differences between fiber bonding conducted using scanning electron microscope (SEM) and ImageJ software. The raw data findings supplement chemical composition analysis for both untreated and treated substrates on handsheet quality performance check as presented in the research article "Bio-Mechanical Pulping of Bacteria Pre-Treatment on Oil Palm Biomass for Handsheet Production" [1]. For understanding correlations into the difference among lignocellulose content composition which affect the handsheet formation and mechanical strength refer to article from this research [1]. This dataset is made publicly available for optimizing alternative waste material reuse in the pulp and paper industrial section.

Matched MeSH terms: Plant Leaves

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