Displaying publications 601 - 620 of 3987 in total

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  1. Tan GH, Vijayaletchumy K
    Bull Environ Contam Toxicol, 1994 Sep;53(3):351-6.
    PMID: 7919710
    Matched MeSH terms: Fresh Water; Water Pollutants, Chemical/analysis*
  2. Ismail BS, Ngan CK, Cheah UB, Abdullah WY
    Bull Environ Contam Toxicol, 2004 Apr;72(4):836-43.
    PMID: 15200001 DOI: 10.1007/s00128-004-0320-5
    Matched MeSH terms: Water Movements; Water Pollutants, Chemical/analysis*
  3. Heng LY, Jusoh K, Ling CH, Idris M
    Bull Environ Contam Toxicol, 2004 Feb;72(2):373-9.
    PMID: 15106775
    Matched MeSH terms: Water Pollutants/pharmacokinetics; Water Pollutants/toxicity*
  4. Marghany M
    J Environ Sci (China), 2004;16(1):44-8.
    PMID: 14971450
    RADARSAT data have a potential role for coastal pollution monitoring. This study presents a new approach to detect and forecast oil slick trajectory movements. The oil slick trajectory movements is based on the tidal current effects and Fay's algorithm for oil slick spreading mechanisms. The oil spill trajectory model contains the integration between Doppler frequency shift model and Lagrangian model. Doppler frequency shift model implemented to simulate tidal current pattern from RADARSAT data while the Lagrangian model used to predict oil spill spreading pattern. The classical Fay's algorithm was implemented with the two models to simulate the oil spill trajectory movements. The study shows that the slick lengths are effected by tidal current V component with maximum velocity of 1.4 m/s. This indicates that oil slick trajectory path is moved towards the north direction. The oil slick parcels are accumulated along the coastline after 48 h. The analysis indicated that tidal current V components were the dominant forcing for oil slick spreading.
    Matched MeSH terms: Water Movements; Water Pollutants/analysis*
  5. Nor Fadhillah Mohmaed Azmin, N Shofia A’yun Syafie, Azlin Suhaida Azmi, Mimi Fina Hamidon, Ani Liza Asnawi
    MyJurnal
    Sg. Papar is one of the rivers in Kota Kinabalu which is mainly used for water supply especially in Papar district. For the past years, many pollution cases concerning Sg. Papar have been reported which originated from various sources including pig farm, agricultural run-off and deforestation. These resulted in a frequent shutdown of the water treatment plants in Papar district leading to water supply disturbance and water supply deficiency in the affected area. The data utilized in this study were obtained from water quality tests performed on river water samples taken from Limbahau water treatment plant recorded from September 2013 to September 2016. Principal Component Analysis (PCA) was used in this study to analyze and correlate the physicochemical parameters with the water treatment plant shutdown. The results revealed that eight parameters (pH, alum, nitrate, TDS, DO, conductivity, colour and chloride) analysed in this study correlate with each other and the parameter that mostly caused the drastic change in the river water and as pollution index is turbidity. This study is critical for understanding the relationship between the water quality paramters and environmental issues.
    Matched MeSH terms: Water Supply; Water Purification; Water Quality
  6. Attias E, Thomas D, Sherman D, Ismail K, Constable S
    Sci Adv, 2020 Nov;6(48).
    PMID: 33239299 DOI: 10.1126/sciadv.abd4866
    Conventional hydrogeologic framework models used to compute ocean island sustainable yields and aquifer storage neglect the complexity of the nearshore and offshore submarine environment. However, the onshore aquifer at the island of Hawai'i exhibits a notable volumetric discrepancy between high-elevation freshwater recharge and coastal discharge. In this study, we present a novel transport mechanism of freshwater moving from onshore to offshore through a multilayer formation of water-saturated layered basalts with interbedded low-permeability layers of ash/soil. Marine electromagnetic imaging reveals ∼35 km of laterally continuous resistive layers that extend to at least 4 km from west of Hawai'i's coastline, containing about 3.5 km3 of freshened water. We propose that this newly found transport mechanism of fresh groundwater may be the governing mechanism in other volcanic islands. In such a scenario, volcanic islands worldwide can use these renewable offshore reservoirs, considered more resilient to climate change-driven droughts, as new water resources.
    Matched MeSH terms: Fresh Water; Water; Groundwater; Water Resources
  7. Rashid SS, Liu YQ
    Sci Total Environ, 2021 Feb 20;756:143849.
    PMID: 33248794 DOI: 10.1016/j.scitotenv.2020.143849
    The occurrence of various micropollutants such as pharmaceuticals personal care products, endocrine disrupting chemicals (PPCPs/EDCs) and metals in municipal wastewater, and their poor removal efficiencies can lead to toxicity impact on humans, and freshwater and terrestrial ecosystems. Life cycle assessment is an efficient and effective tool to evaluate the environmental impact of wastewater treatment plants, but guidelines for toxicity assessment are lacking due to the complexity. This study aims to evaluate both life cycle inventory by including metals and PEC, and life cycle toxicity assessment (LCIA) methods namely CML-IA, Recipe, USEtox, EDIP 2003 and IMPACT 2002+ in midpoint category with a large centralised wastewater treatment plant in Malaysia as a case study. The removal efficiencies of metals and PPCPs/EDCs in the wastewater ranged from 9% to 99% and no clear patterns were found about occurrence and removal efficiencies of metals and PPCPs/EDCs in developing and developed countries. The inclusion of metals and PPCPs/EDCs in effluent resulted in 76% increase in freshwater ecotoxicity potential (FEP) and 88% increase in terrestrial ecotoxicity potential (TEP) while only 4% increase in human toxicity potential (HTP). The results indicate the importance of including direct emissions such as metals and PPCPs/EDCs even in low-strength municipal wastewater for environmental toxicity assessment. The comparison of five LCIA methods suggests that HTP assessment is more challenging due to inconsistency between five LCIA methods while CML-IA, Recipe, and IMPACT 2002+ achieved consistent human toxicity and ecotoxicity assessment results in the WWTP. The results highlight the importance of sampling and inclusion of metals and PPCPs/EDCs data especially prioritised micropollutants for life cycle toxicity assessment and recommends LCIA methods for ecotoxicity assessment of WWTPs in the current scientific development situation on toxicity studies, which can provide guidance to researchers for life cycle toxicity assessment of wastewater treatment.
    Matched MeSH terms: Water Purification*; Waste Water
  8. Thiruvenkadam S, Izhar S, Hiroyuki Y, Harun R
    Biomed Res Int, 2018;2018:1931634.
    PMID: 30533428 DOI: 10.1155/2018/1931634
    Subcritical water extraction (SCW) was used to extract oil from Chlorella pyrenoidosa. The operational factors such as reaction temperature, reaction time, and biomass loading influence the oil yield during the extraction process. In this study, response surface methodology was employed to identify the desired extraction conditions for maximum oil yield. Experiments were carried out in batch reactors as per central composite design with three independent factors including reaction temperature (170, 220, 270, 320, and 370°C), reaction time (1, 5, 10, 15, and 20 min), and biomass loading (1, 3, 5, 10, and 15%). A maximum oil yield of 12.89 wt.% was obtained at 320°C and 15 min, with 3% biomass loading. Sequential model tests showed the good fit of experimental data to the second-order quadratic model. This study opens the great potential of SCW to extract algal oil for use in algal biofuel production.
    Matched MeSH terms: Fresh Water; Water/chemistry*
  9. Arumugam N, Chelliapan S, Kamyab H, Thirugnana S, Othman N, Nasri NS
    PMID: 30551682 DOI: 10.3390/ijerph15122851
    Inadequately treated or untreated wastewater greatly contribute to the release of unwanted toxic contaminants into water bodies. Some of these contaminants are persistent and bioaccumulative, becoming a great concern as they are released into the environment. Despite the abundance of wastewater treatment technologies, the adsorption method overall has proven to be an excellent way to treat wastewater from multiple industry sources. Because of its significant benefits, i.e., easy availability, handling, and higher efficiency with a low cost relative to other treatments, adsorption is opted as the best method to be used. However, biosorption using naturally found seaweeds has been proven to have promising results in removing pollutants, such as dyes from textile, paper, and the printing industry, nitrogen, and phosphorous and phenolic compounds, as well as heavy metals from various sources. Due to its ecofriendly nature together with the availability and inexpensiveness of raw materials, biosorption via seaweed has become an alternative to the existing technologies in removing these pollutants from wastewater effectively. In this article, the use of low-cost adsorbent (seaweed) for the removal of pollutants from wastewater has been reviewed. An extensive table summarises the applicability of seaweed in treating wastewater. Literature reported that the majority of research used simulated wastewater and minor attention has been given to biosorption using seaweed in the treatment of real wastewater.
    Matched MeSH terms: Water Pollutants, Chemical/isolation & purification*; Water Pollutants, Chemical/metabolism*
  10. J.Karim, M.A.M.Idrus, N.H.F.Hashim, M.Abdullah, S.S.Sharifuddin, M.F.Muhazeli, et al.
    MyJurnal
    Rivers as surface water in Malaysia are recipients of effluents and wastewater and yet it is important water source for daily uses of some villagers living along the river. Endocrine disruptors such as Bisphenol A (BPA) can be found in river due to continuous discharge into it. The objectives of this research is to find out the occurrence and concentration of BPA in Sungai Langat and also to see how water quality parameters such as temperature, pH, dissolved oxygen (DO), turbidity, Total Suspended Solid (TSS), Biochemical Oxygen Demand (BOD), Chemical Oxygen Demand (COD) and ammonia nitrogen (AN) affecting the concentration of BPA. 12 stations in total including upstream to downstream of Sungai Langat and also tributary of Sungai Langat. The instrument used to find out concentration of BPA is Triple Quadrupole LC/MS. The source of BPA are mainly industrial effluents and also direct domestic discharges. The water quality parameters that will affect concentration of BPA are Ammonia Nitrogen (AN), turbidity, Biochemical Oxygen Demand (BOD), Total Suspended Solid (TSS), and Chemical Oxygen Demand (COD), Dissolved Oxygen (DO). While pH and water temperature are also factors that will affect concentration of BPA but the significance is not shown in the analysis. It can be concluded that upstream of Sungai Langat has lower concentration of BPA than downstream.
    Matched MeSH terms: Water; Water Quality; Waste Water
  11. Yi X, Yin S, Huang L, Li H, Wang Y, Wang Q, et al.
    Sci Total Environ, 2021 Jun 01;771:144644.
    PMID: 33736175 DOI: 10.1016/j.scitotenv.2020.144644
    Chlorine radical plays an important role in the formation of ozone and secondary aerosols in the troposphere. It is hence important to develop comprehensive emissions inventory of chlorine precursors in order to enhance our understanding of the role of chlorine chemistry in ozone and secondary pollution issues. Based on a bottom-up methodology, this study presents a comprehensive emission inventory for major atomic chlorine precursors in the Yangtze River Delta (YRD) region of China for the year 2017. Four primary chlorine precursors are considered in this study: hydrogen chloride (HCl), fine particulate chloride (Cl-) (Cl- in PM2.5), chlorine gas (Cl2), and hypochlorous acid (HClO) with emissions estimated for twelve source categories. The total emissions of these four species in the YRD region are estimated to be 20,424 t, 15,719 t, 1556 and 9331 t, respectively. The emissions of HCl are substantial, with major emissions from biomass burning and coal combustion, together accounting for 68% of the total HCl emissions. Fine particulate Cl- is mainly emitted from industrial processing, biomass burning and waste incineration. The emissions of Cl2 and HClO are mainly associated with usage of chlorine-containing disinfectants, for example, water treatment, wastewater treatment, and swimming pools. Emissions of each chlorine precursor are spatially allocated based on the characteristics of individual source category. This study provides important basic dataset for further studies with respect to the effects of chlorine chemistry on the formation of air pollution complex in the YRD region.
    Matched MeSH terms: Water; Water Purification; Waste Water
  12. Tee HS, Lee CY
    J Insect Physiol, 2015 Jun;77:45-54.
    PMID: 25921676 DOI: 10.1016/j.jinsphys.2015.04.007
    The impact of desiccation on habitat selection, foraging and survival has been characterized for many insects. However, limited information is available for parasitic wasps. In this study, water balance, relative humidity (RH) preference, and effect of humidity on survival of solitary Evania appendigaster (L.) (Hymenoptera: Evaniidae) and gregarious Aprostocetus hagenowii (Ratzeburg) (Hymenoptera: Eulophidae) were examined. These species are both oothecal parasitoids of the American cockroach Periplaneta americana (L.) (Dictyoptera: Blattidae). E. appendigaster had significantly higher cuticular permeability (CP) and a lower surface area to volume ratio but a similar percentage of total body water content compared to A. hagenowii. No differences in these attributes were found between sexes of each parasitoid species. The percentage of total body water loss rates among E. appendigaster males and females and A. hagenowii females were similar but significantly lower than that of A. hagenowii males. All parasitoids except E. appendigaster males exhibited reduced survival times as the RH of their enclosure decreased from 87% to 38%, but this phenomenon did not occur when parasitoids were given a sugar solution. In environmental chambers with a 44-87% RH gradient, both sexes of E. appendigaster resided significantly more often in the 87% RH chamber than in the 44% RH chamber. For A. hagenowii, females preferred both the driest and the wettest chambers and males preferred the driest ones. These results demonstrate the water balance profile and its relationship to life history traits and differential responses to RH in these competing parasitoid wasps, suggesting the role of physiological and behavioral adaptations in shaping their ecological niche.
    Matched MeSH terms: Body Water; Water-Electrolyte Balance/physiology*
  13. Hasan H, Parker A, Pollard SJT
    Sci Total Environ, 2021 Feb 10;755(Pt 1):142868.
    PMID: 33348485 DOI: 10.1016/j.scitotenv.2020.142868
    We explore the interplay between preventative risk management and regulatory style for the implementation of water safety plans in Malaysia and in England and Wales, two jurisdictions with distinct philosophies of approach. Semi-structured interviews were conducted with 32 water safety professionals in Malaysia, 23 in England and Wales, supported by 6 Focus Group Discussions (n = 53 participants). A grounded theory approach produced insights on the transition from drinking water quality surveillance to preventative risk management. Themes familiar to this type of regulatory transition emerged, including concerns about compliance policy; overseeing the risk management controls of regulatees with varied competencies and funds available to drive change; and the portfolio of interventions suited to a more facilitative regulatory style. Because the potential harm from waterborne illness is high where pathogen exposures occur, the transition to risk-informed regulation demands mature organisational cultures among water utilities and regulators, and a laser-like focus on ensuring risk management controls are delivered within water supply systems.
    Matched MeSH terms: Water Supply; Water Quality; Drinking Water
  14. Zahidi I, Wilson G, Brown K, Hou FKK
    J Health Pollut, 2020 Dec;10(28):201207.
    PMID: 33324504 DOI: 10.5696/2156-9614-10.28.201207
    Background: Rivers are susceptible to pollution and water pollution is a growing problem in low- and middle-income countries (LMIC) with rapid development and minimal environmental protections. There are universal pollutant threshold values, but they are not directly linked to river activities such as sand mining and aquaculture. Water quality modelling can support assessments of river pollution and provide information on this important environmental issue.

    Objectives: The objective of the present study was to demonstrate water quality modelling methodology in reviewing existing policies for Malaysian river catchments based on an example case study.

    Methods: The MIKE 11 software developed by the Danish Hydraulic Institute was used to model the main pollutant point sources within the study area - sand mining and aquaculture. Water quality data were obtained for six river stations from 2000 to 2015. All sand mining and aquaculture locations and approximate production capacities were quantified by ground survey. Modelling of the sand washing effluents was undertaken with the advection-dispersion module due to the nature of the fine sediment. Modelling of the fates of aquaculture deposits required both advection-dispersion and Danish Hydraulic Institute ECO Lab modules to simulate the detailed interactions between water quality determinants.

    Results: According to the Malaysian standard, biochemical oxygen command (BOD) and ammonium (NH4) parameters fell under Class IV at most of the river reaches, while the dissolved oxygen (DO) parameter varied between Classes II to IV. Total suspended solids (TSS) fell within Classes IV to V along the mid river reaches of the catchment.

    Discussion: Comparison between corresponding constituents and locations showed that the water quality model reproduced the long-term duration exceedance for the main body of the curves. However, the water quality model underestimated the infrequent high concentration observations. A standard effluent disposal was proposed for the development of legislation and regulations by authorities in the district that could be replicated for other similar catchments.

    Conclusions: Modelling pollutants enables observation of trends over the years and the percentage of time a certain class is exceeded for each individual pollutant. The catchment did not meet Class II requirements and may not be able to reach Class I without extensive improvements in the quality and reducing the quantity of both point and non-point effluent sources within the catchment.

    Competing Interests: The authors declare no competing financial interests.

    Matched MeSH terms: Water; Water Pollution; Water Quality
  15. Cheng SY, Show PL, Juan JC, Chang JS, Lau BF, Lai SH, et al.
    Chemosphere, 2021 Jan;262:127829.
    PMID: 32768754 DOI: 10.1016/j.chemosphere.2020.127829
    Recent trend to recover value-added products from wastewater calls for more effective pre-treatment technology. Conventional landfill leachate treatment is often complex and thus causes negative environmental impacts and financial burden. In order to facilitate downstream processing of leachate wastewater for production of energy or value-added products, it is pertinent to maximize leachate treatment performance by using simple yet effective technology that removes pollutants with minimum chemical added into the wastewater that could potentially affect downstream processing. Hence, the optimization of coagulation-flocculation leachate treatment using multivariate approach is crucial. Central composite design was applied to optimize operating parameters viz. Alum dosage, pH and mixing speed. Quadratic model indicated that the optimum COD removal of 54% is achieved with low alum dosage, pH and mixing speed of 750 mgL-1, 8.5 and 100 rpm, respectively. Optimization result showed that natural pH of the mature landfill leachate sample is optimum for alum coagulation process. Hence, the cost of pH adjustment could be reduced for industrial application by adopting optimized parameters. The inherent mechanism of pollutant removal was elucidated by FTIR peaks at 3853 cm-1 which indicated that hydrogen bonds play a major role in leachate removal by forming well aggregated flocs. This is concordance with SEM image that the floc was well aggregated with the porous linkages and amorphous surface structure. The optimization of leachate treatment has been achieved by minimizing the usage of alum under optimized condition.
    Matched MeSH terms: Water Pollutants, Chemical/analysis*; Waste Water
  16. Ahmed, Moussa Mohamed, Nik Rashida Nik Abdul Ghani, Jami, Mohammed Saedi, Mirghani, Mohamed Elwathig Saeed, Md. Noor Salleh
    MyJurnal
    Boron has been classified as a drinking water pollutant in many countries. It is harmful to many plants, exceptionally sensible plants, and human health. Therefore, boron level needs to be decreased to 0.3 mg/L for drinking water and within 0.5 mg/L to 1 mg/L for irrigation water. In this study, various operational parameters namely pH, contact time and liquid/solid ratio were investigated to determine the potential of using date seed (or date pit or date stone) to remove boron from seawater. This study's main objective was to determine boron adsorption capacities of date seeds prepared by various methods (i.e., powdered, activated, acid-treated and defatted seed) by batch adsorption process using boron contaminated synthetic seawater. The process parameters of the selected biosorbent among the four date seed preparations methods were optimized. The surface characteristics were analyzed by using Fourier Transform Infrared Spectroscopy (FTIR) and Scanning Electron Microscope (SEM). The results showed that acid-treated date seed was the best biosorbent in terms of removing 89.18% boron from aqueous solution at neutral pH, liquid to solid ratio of 5 within 2 hours of reaction time at room temperature (25°C±2°C).
    Matched MeSH terms: Water Pollutants; Water Pollution; Drinking Water
  17. Adib MNM, Rowshon MK, Mojid MA, Habibu I
    Sci Rep, 2020 05 20;10(1):8336.
    PMID: 32433561 DOI: 10.1038/s41598-020-65114-w
    Climate change-induced spatial and temporal variability of stremflow has significant implications for hydrological processes and water supplies at basin scale. This study investigated the impacts of climate change on streamflow of the Kurau River Basin in Malaysia using a Climate-Smart Decision Support System (CSDSS) to predict future climate sequences. For this, we used 25 reliazations consisting from 10 Global Climate Models (GCMs) and three IPCC Representative Concentration Pathways (RCP4.5, RCP6.0 and RCP8.5). The generated climate sequences were used as input to Soil and Water Assessment Tool (SWAT) to simulate projected changes in hydrological processes in the basin over the period 2021-2080. The model performed fairly well for the Kurau River Basin, with coefficient of determination (R2), Nash-Sutcliffe Efficiency (NSE) and Percent Bias (PBIAS) of 0.65, 0.65 and -3.0, respectively for calibration period (1981-1998) and 0.60, 0.59 and -4.6, respectively for validation period (1996-2005). Future projections over 2021-2080 period show an increase in rainfall during August to January (relatively wet season, called the main irrigation season) but a decrease in rainfall during February to July (relatively dry season, called the off season). Temperature projections show increase in both the maximum and minimum temperatures under the three RCP scenarios, with a maximum increase of 2.5 °C by 2021-2080 relative to baseline period of 1976-2005 under RCP8.5 scenario. The model predicted reduced streamflow under all RCP scenarios compared to the baseline period. Compared to 2021-2050 period, the projected streamflow will be higher during 2051-2080 period by 1.5 m3/s except in February for RCP8.5. The highest streamflow is predicted during August to December for both future periods under RCP8.5. The seasonal changes in streamflow range between -2.8% and -4.3% during the off season, and between 0% (nil) and -3.8% during the main season. The assessment of the impacts of climatic variabilities on the available water resources is necessary to identify adaptation strategies. It is supposed that such assessment on the Kurau River Basin under changing climate would improve operation policy for the Bukit Merah reservoir located at downstream of the basin. Thus, the predicted streamflow of the basin would be of importance to quantify potential impacts of climate change on the Bukit Merah reservoir and to determine the best possible operational strategies for irrigation release.
    Matched MeSH terms: Water; Water Supply; Water Resources
  18. Alexander AD, Evans LB, Baker MF, Baker HJ, Ellison D, Marriapan M
    Appl Microbiol, 1975 Jan;29(1):30-3.
    PMID: 1110490
    Pathogenic leptospiras (1,424) isolated from natural waters and wet soils in Malaysia comprised 29 different serovars (synonym serotypes). All except two of the serovars had been found previously in Malaysia. The exceptional serovars were werrasingha, an Autumnalis serogroup member originally isolated in Ceylon, and a new serovar designated evansi. Serovar evansi had serological affinities with serovar ranarum which was isolated from the kidney of a frog in Iowa. The large variety of serovars found in jungle areas was consistent with similar previous findings of diverse serovar infections in troops who had operated in Malaysian jungles.
    Matched MeSH terms: Fresh Water; Water Microbiology*
  19. Revathi M, Sivagaami Sundari G, Ahmed Basha C, Alam M, Sagadevan S, Ahmad N
    J Nanosci Nanotechnol, 2020 10 01;20(10):6547-6554.
    PMID: 32385012 DOI: 10.1166/jnn.2020.18562
    This investigation aims at the reclamation of Cr(VI) from synthetic electroplating industrial effluent by electroextraction process namely electrochemical ion exchange (EIX). An electrochemical ion exchange reactor of desired dimensions was fabricated with the help of ion-permeable membranes, stainless steel cathode and PbO₂ coated Ti expanded mesh anode. The performance of the reactor was studied in batch recirculation mode, continuous flow mode at different experimental conditions. The influence of various experimental factors, for instance, initial metal ion concentration (20, 300, 1000 mg/L of Cr(VI)), applied voltages (2.5 V, 5 V, 7.5 V, 10 V) and flow rates of the process stream (2, 4, 6, 8, 10, 12 and 14 ml/min) on removal/reclamation efficiency was deliberated. For comparison purposes, an electrodialysis process was conducted at the same optimal conditions. It was found that the EIX process with three compartments has more removal efficiency at optimum experimental conditions than the electrodialysis process. The continuous flow process of the reactor with 300 mg/L of Cr(VI) as inlet concentration has studied to predict the breakeven point of the reactor. It was noted that Cr(VI) ion concentration in the treated wastewater is almost zero up to the discharge of 20 liters of treated rinse water.
    Matched MeSH terms: Water; Water Purification; Waste Water
  20. Chávez M, Cabezas AF, Ferauds M, Castillo JE, Caicedo LD
    Trop Biomed, 2020 Sep 01;37(3):650-662.
    PMID: 33612779 DOI: 10.47665/tb.37.3.650
    Pseudomonas aeruginosa is considered an opportunistic pathogen, causing a wide variety of infections in compromised hosts, also frequently develops multi-resistance to antibiotics and can colonize various habitats, including water systems. The main aim of this study was to investigate antibiotics susceptibility pattern, genotypic diversity and detection of resistence genes in P. aeruginosa isolates from clinical and aquatic environment sources. Of the 220 P. aeruginosa isolates examined, 48 were clinical isolates and 172 isolates from wastewater and freshwater. Susceptibility to eight antimicrobial agents was carried out by disk diffusion method. Clinical and environmental isolates were screened for the presence of the genes encoding blaKPC-2, blaCTX-M-9, blaPER-1, blaOXA-10, blaIMP-1, blaVIM-2 and blaampC by polymerase chain reaction (PCR). Isolates were examined with PCR-SSCP analysis of partial DNAr 16S sequence. Isolates were mainly resistant to cefoxitin. Multidrug-resistant P. aeruginosa (MDRPA) strains were found in 70% and 90.3% of the clinical and environmental isolates, respectively. The prevalence rates of â-lactamase genes were recorded (blaKPC-2 41.3%, blaVIM-2 36.8%, blaIMP-1 13.6%, blaCTX-M-9 10.9% and blaampC 10.5%,). The PCR-SSCP analysis showed three conformational patterns. All clinical isolates and most environmental isolates were grouped into a single cluster. In this study, we found that P. aeruginosa strains recovered from city water systems must be considered potential reservoir for ESBL genes, especially blaKPC-2 and blaVIM-2.
    Matched MeSH terms: Fresh Water; Water; Waste Water
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