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  1. Tan MK, Kamaruddin KN
    Zootaxa, 2016 May 12;4111(1):21-40.
    PMID: 27394894 DOI: 10.11646/zootaxa.4111.1.2
    Bukit Larut is a hill station at the southern tip of the Bintang Range, Perak of Peninsular Malaysia. While the biodiversity of Bukit Larut has been previously documented, its entomofauna, including the Orthoptera, remains relatively unknown. A faunistic survey was conducted in 2015 as part of the continuous exploration of the highlands in Malay Peninsula. An annotated species list of 71 (24 Caelifera and 47 Ensifera) species of Orthoptera from ten families (five from each order) is presented here. While the coverage of lineages in the orthopteran phylogeny is well-represented, the diversity in Bukit Larut is dominated by the three main families: Acrididae, Gryllidae and Tettigoniidae. Eight new locality records for Bukit Larut and/or Peninsular Malaysia and potential new species awaiting description highlight that the orthopteran diversity in Bukit Larut is not exhaustive.
  2. Tan TL, Goh YY
    PLoS One, 2017;12(7):e0180554.
    PMID: 28671974 DOI: 10.1371/journal.pone.0180554
    INTRODUCTION: This paper investigates the role of Group II Secretory Phospholipase A2 (sPLA2-IIA) as a biomarker for the diagnosis of sepsis and bacterial infection in adults. Sepsis and bacterial infection are common problems encountered by patients in the hospital and often carry adverse outcomes if not managed early.

    METHODS: Two independent reviewers conducted a comprehensive search using Ovid MEDLINE published from years 1993 to 2016 and SCOPUS published from year 1985 to 2017 to screen for relevant studies. The main inclusion criteria included adult subjects, patients with suspected or confirmed signs of infection and relevant outcomes which looked into the role of sPLA2-IIA in detecting the presence of sepsis and bacterial infection in the subjects.

    RESULTS AND DISCUSSION: Four studies met the inclusion criteria. SPLA2-IIA was found to be effective in detecting the presence of sepsis and bacterial infection in adults. The levels of serum sPLA2-IIA also correlated well with the presence of sepsis and bacterial infection.

    CONCLUSION: This systematic review highlights the role of sPLA2-IIA as a reliable tool to diagnose sepsis and bacterial infection in adult patients. Nonetheless, further studies should be done in the future to provide more compelling evidence on its application in the clinical setting.

  3. Tan YL, Wee TC
    Med J Malaysia, 2017 12;72(6):372-373.
    PMID: 29308778
    We report a rare case of adult human metapneumovirus (HMPV) in a healthy 32-year-old man. There was dramatic deterioration in his condition developing pneumonia with Type-I respiratory failure and encephalitis. He needed mechanical ventilation in the intensive care setting and was treated with intravenous ribavirin. Post-extubation he remained severely physically and cognitively impaired despite rehabilitation. Treatment of HMPV pneumonia is at present, still without specific antiviral therapy. Managing HMPV-encephalitis remained supportive and challenging. More definite treatment strategies are needed.
  4. Ho CC, Tan HM
    Sex Med Rev, 2013 May;1(1):42-49.
    PMID: 27784559 DOI: 10.1002/smrj.4
    INTRODUCTION: Testosterone treatment for hypogonadism is detrimental for men in reproductive age as it impairs spermatogenesis, and therefore affects fertility. It is, therefore, not indicated in men with hypogonadism and infertility.

    AIM: The aim of this review is to analyze current data regarding options of treatment for men with hypogonadism and infertility.

    MAIN OUTCOMES MEASURES: A comprehensive review of the current literature on management of infertility among hypogonadal men.

    METHODS: A literature search using PubMed from 1980 to 2012 was done on articles published in the English language. The following medical subject heading terms were used: "infertility," "infertile," "hypogonadism;" "testosterone deficiency" and "men" or "male;" and "treatment" or "management."

    RESULTS: The options for hypogonadal testicular failure are limited. Hormonal treatment is by and large ineffective. For secondary hypogonadism (hypogonadotropic/normogonadotropic hypogonadism), the options include gonadotropin-releasing hormone, human chorionic gonadotropin (hCG), human menopausal gonadotropin (hMG), follicle-stimulating hormone (FSH), and anti-estrogens and aromatase inhibitors. Dopamine antagonist is indicated for prolactinoma. Artificial reproductive technique is indicated for primary testicular failure and also when medical therapy fails.

    CONCLUSION: The most suitable option with the current data available is hCG with or without hMG/FSH. Testosterone supplementation should be avoided, but if they are already on it, it is still possible for a return of normal sperm production within 1 year after discontinuing testosterone. Ho CCK and Tan HM. Treatment of the hypogonadal infertile male-A review. Sex Med Rev 2013;1:42-49.

  5. Ng ZY, Tan GYA
    Antonie Van Leeuwenhoek, 2018 May;111(5):727-742.
    PMID: 29511956 DOI: 10.1007/s10482-018-1042-8
    Tioman Island is one of many sources for underexplored actinobacterial diversity in Malaysia. Selective isolation, molecular profiling, 16S rRNA gene sequencing and phylogenetic analyses were carried out to highlight the diversity of the marine actinobacterial community in a sediment collected off Tioman Island. A high number of diverse actinobacteria were recovered using skim milk/HEPES pre-treatment on a mannitol-based medium. A total of 123 actinobacterial strains were isolated, including thirty obligate marine actinobacteria putatively identified as Salinispora spp. Molecular fingerprinting profiles obtained with a double digestion approach grouped the remaining non-Salinispora-like strains into 24 different clusters, with Streptomyces and Blastococcus as the major clusters. A total of 17 strains were identified as novel actinobacterial species within the genera Streptomyces (n = 6), Blastococcus (n = 5), Marinactinospora (n = 3), Nocardiopsis (n = 1), Agromyces (n = 1) and Nonomuraea (n = 1) based on 16S rRNA gene sequence analyses. Polyphasic data from three putative Marinactinospora spp. showed that the strains represent a new genus in the Nocardiopsaceae family. Crude extracts from the strains were also found to inhibit the growth of Gram-positive (Staphylococcus aureus, Bacillus subtilis) and Gram-negative (Providencia alcalifaciens) pathogens. Hierarchical clustering of the bioactivities of an active fraction revealed a unique profile, which is closely related that of fosfomycin.
  6. Tan CS, Yam MF
    Naunyn Schmiedebergs Arch Pharmacol, 2018 06;391(6):561-569.
    PMID: 29552696 DOI: 10.1007/s00210-018-1481-9
    Previous studies have demonstrated that 3'-hydroxy-5,6,7,4'-tetramethoxyflavone (TMF) content in Orthosiphon stamineus fractions correlate with its vasorelaxation activity. Even with the availability of previous studies, there is still very little information on the vasorelaxation effect of TMF, and few scientific studies have been carried out. Therefore, the present study was designed to investigate the vasorelaxation activity and mechanism of action of the TMF. The vasorelaxation activity and the underlying mechanisms of TMF were evaluated on thoracic aortic rings isolated from Sprague Dawley rats. TMF caused the relaxation of aortic rings with endothelium pre-contracted with phenylephrine. However, the vasorelaxant effect of TMF was significantly decreased in PE-primed endothelium-denuded and potassium chloride-primed endothelium-intact aortic rings. In the presence of Nω-nitro-L-arginine methyl ester, methylene blue, 1H-[1,2,4]oxadiazolo[4,3-a]quinoxalin-1-one, indomethacin, tetraethylammonium, 4-aminopyridine, barium chloride, atropine and propranolol, the relaxation stimulated by TMF was significantly reduced. TMF was also found to reduce Ca2+ release from sarcoplasmic reticulum (via IP3R) and block calcium channels (VOCC). The present study demonstrates the vasorelaxant effect of TMF involves NO/sGC/cGMP and prostacyclin pathways, calcium and potassium channels and muscarinic and beta-adrenergic receptors.
  7. Lim HM, Tan KS
    J Rubber Res, 2022;25(5):413-419.
    PMID: 36320319 DOI: 10.1007/s42464-022-00184-1
    Commercially available carboxylated acrylonitrile butadiene latex (XNBR) was physically blended with natural rubber latex (NR) at varying blend ratios to investigate its effect on the mechanical properties and morphology. Methyl methacrylate grafted natural rubber latex (MG) was added to the latex blends as a third polymer to study whether it could enhance the mechanical properties of the latex blend films. It was found that the tensile strength of the blend films irrespective of composition decreased when the two latexes were blended as compared to the virgin latex films. The modulus 300 decreased while the elongation at break and tear strength of the blend films increased gradually as the ratio of NR increased in the blend films. It was found that the MG did not enhance the mechanical properties of the XNBR/NR blend films under the current experimental condition. AFM phase imaging analysis revealed enhanced polymer distribution and evidence of NR-MG-XNBR interactions.
  8. Tan ST, Kannan TM
    Dialogues Health, 2023 Dec;2:100094.
    PMID: 36568862 DOI: 10.1016/j.dialog.2022.100094
    BACKGROUND AND PURPOSE: The enforcement of nationwide lockdowns has worsened the obesity epidemic in Malaysia. Therefore, this study aims to compare eating behaviour by relative weight change status among young adults in Malaysia throughout the COVID-19 pandemic.

    DESIGN/METHODOLOGY/APPROACH: Socio-demographics, body height, pre-pandemic body weight, and post-lockdown body weight were self-reported by the young adults. The eating behaviour was assessed using the validated Three-Factor Eating Questionnaire-Revised 18-item (TFEQ-R18). The mean difference in eating behaviour subscales was analysed using MANCOVA with a Bonferroni-adjusted post-hoc test.

    FINDINGS: One-third of the young adults put on body weight due to the lockdowns, with an average relative weight gain of 12.44 ± 9.67%. Conversely, about one-fifth of the young adults reported having lighter body weight in the post-lockdown pandemic phase, with an average relative weight loss of 10.10 ± 4.66%. Young adults in the weight loss category had a significantly higher standardised raw score in cognitive restraints than those in the sustained weight and weight gain categories. In addition, young adults in the weight gain category had statistically higher standardised raw scores in uncontrolled eating and emotional eating compared to those in the sustained weight category. Weight trajectory during the COVID-19 pandemic is linked to disordered eating behaviour among young adults in Malaysia.

    ORIGINALITY/VALUE: The findings presented in this study can be potentially valuable in formulating weight management strategies in the post-COVID-19 era.

  9. Tan GC, Wong YP
    Malays J Pathol, 2022 Dec;44(3):365.
    PMID: 36591706
    No abstract available.
  10. Tan SG, Xu PY
    Trop Biomed, 2022 Dec 01;39(4):524-530.
    PMID: 36602211 DOI: 10.47665/tb.39.4.007
    Canine babesiosis caused by Babesia spp. is a noteworthy tick-borne zoonotic disease of domestic dogs and wild canids. In present study, a total of 556 blood samples were randomly collected from pet dogs in eight cities of Hunan province, subtropical China. Genomic DNA was extracted and Babesia DNA was detected by amplification of partial 18S rRNA gene sequences. A total of 56 (10.1%) blood samples were tested positive for Babesia species. Sequence analysis showed that 29 dogs (5.2%) were positive for B. gibsoni, and other 27 dogs for B. vogeli (4.9%). The age and health status were considered as important risk factors for B. gibsoni and B. vogeli infections in pet dogs in this study (P<0.05). Phylogenetic analysis showed that the examined positive samples were highly clustered in the same branch with B. gibsoni and B. vogeli, respectively. This is the first molecular report of B. gibsoni infection in pet dogs in Hunan province, subtropical China. Our finding has provided a guide for the control of dog babesiosis in China and elsewhere.
  11. Tong SF, Tan CE
    Malays Fam Physician, 2022 Nov 30;17(3):2-8.
    PMID: 36606174 DOI: 10.51866/cm0004
    Evidence-based medicine is the foundation of current medical practice. Suitable evidence is needed to support the holistic approach in clinical practice. Quantitative research produces some evidence needed for disease treatment based on probabilities or averages. However, the practice of evidence-based medicine should be personalised to individual patients without relying solely on an average perspective. Beliefs, values and expectations are unique for each individual and may differ significantly from the average. Therefore, understanding individual differences requires evidence from qualitative research. This is particularly important in family medicine practice, which focuses on holistic care for the person, family and community. Findings from properly conducted qualitative research can offer in-depth and comprehensive accounts on healthcare issues from patient and practice perspectives. Qualitative research also provides explanatory power and analytical transferability, which can be applied into daily family medicine practice. In conclusion, evidence from qualitative research should be rightfully equally acknowledged in family medicine and healthcare.
  12. Tan KK, Chin CN
    Singapore Med J, 1996 Dec;37(6):668-9.
    PMID: 9104074
    Unilateral pulmonary agenesis is a rare disorder and is an unusual cause of respiratory distress in the newborn. It is often associated with other congenital abnormalities, as documented in about 200 cases of unilateral pulmonary agenesis in the current literature. The onset and mode of presentation are highly variable, from asymptomatic cases discovered incidentally to symptomatic cases diagnosed in early infancy. We report a newborn infant with right pulmonary agenesis associated with facial and skeletal abnormalities who presented with respiratory distress. Unilateral pulmonary agenesis should be considered in the differential diagnosis of respiratory distress in the newborn, particularly when there are other associated congenital abnormalities.
  13. Tan KK, Easaw PE
    Singapore Med J, 1995 Jun;36(3):326-7.
    PMID: 8553105
    A 5-year-old Chinese boy presented with difficulty in walking and weakness of his lower limbs for one year, especially towards the evening. Bilateral equinovarus posturing of the feet and tremors of the upper limbs were noted on physical examination. Dopa-responsive dystonia was diagnosed after a remarkable symptomatic response to levodopa. This disorder is reported here to highlight an often misdiagnosed condition is children which is important because it is treatable. Dopa-responsive dystonia should be considered in the differential diagnosis of gait disturbance in children.
  14. Ramli N, Rahmat K, Tan GP
    Singapore Med J, 2008 Jul;49(7):e175-7.
    PMID: 18695851
    Malignant osteopetrosis is associated with petrous carotid canal and internal carotid artery stenosis in the skull base. We present a four-year-old boy with malignant osteopetrosis who developed right frontal lobe infarction as a result of bilateral internal carotid artery hypotrophy.
  15. Tan CT, Rapport RL
    Singapore Med J, 1981 Jun;22(3):140-3.
    PMID: 7302620
    Language studies were done on four multilingual dextral Chinese patients who developed dysphasia from various causes. The left hemisphere appeared to be dominant for all the languages in the four patients. All the languages and dialects were universally involved during the development of dysphasia. In one patient,
    there was evidence of delayed restitution in the patient's mother tongue (Hokkien) comparing with Mandarin and English which were the languages used in the hospital and for reading.
  16. Tan HW, Ch'ng SL
    Singapore Med J, 1991 Jun;32(3):189-90.
    PMID: 1876897
    We report a previously undocumented drug interaction between cyclosporine A and quinine. A 39 year old Asian with a recent renal transplant was diagnosed to have a mild cerebral falciparum malaria. He was treated with seven days of oral quinine (600 mg, 8 hourly), followed by a stat dose of pyrimethamine (75 mg)--sulfadoxime (1200mg) because of a strong suspicion of chloroquine resistant falciparum malaria. Using a polyclonal radioimmunoassay method, we measured morning trough cyclosporine A level before, during and after the quinine treatment. Results showed a gradual decrease in the cyclosporine A level from a baseline value of 328 ng/ml to 107 ng/ml after seven days of oral quinine with a subsequent rise to pre-treatment level after discontinuation of quinine. There was no significant change in the dose of cyclosporine A administered during the period of quinine treatment (4.05 to 3.83 mg/kg body weight). Biochemical liver function tests, serum creatinine and hematological parameters were also essentially unchanged during this period. In vitro study showed no significant methodological interference in the cyclosporine assay by quinine dihydrochloride. These findings suggest an in vivo drug interaction between cyclosporine A and quinine. The mechanism of this interaction is not clear. Further studies are required to confirm the significance of this observation. Quinine and its stereoisomer, quinidine should be used with caution until further information is available.
  17. Tan CT, Chua KB
    Curr Infect Dis Rep, 2008 Jul;10(4):315-20.
    PMID: 18765105
    Nipah virus was first discovered in 1999, after a severe outbreak of viral encephalitis among pig farm workers in Malaysia. The disease is thought to spread from Pteropus bats to pigs and then to humans following close contact. The reported mortality rate in this outbreak was 40%. The main necropsy finding in patients with Nipah virus encephalitis was disseminated microinfarction associated with vasculitis and direct neuronal involvement. Relapse of encephalitis was seen in 10% of those who survived the initial illness. Since that initial report, recurrent outbreaks of Nipah virus encephalitis have been seen in Bangladesh and West Bengal, India. These outbreaks occurred between January and May, with Pteropus giganteus as a reservoir of the virus. In Bangladesh, the virus probably spread directly from bats to humans-with human to human spread as another important mode of infection-and the mortality rate was 70%.
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