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Age-Stage, Two-Sex Life Table Characteristics of Aedes albopictus and Aedes Aegypti in Penang Island, Malaysia

Maimusa HA, Ahmad AH, Kassim NF, Rahim J

J Am Mosq Control Assoc, 2016 Mar;32(1):1-11.
PMID: 27105211 DOI: 10.2987/moco-32-01-1-11.1

The life table developmental attributes of laboratory colonies of wild strains of Aedes albopictus and Aedes aegypti were analyzed and compared based on the age-stage, two-sex life table. Findings inclusive in this study are: adult preoviposition periods, total preoviposition period, mean intrinsic rate of increase (r), mean finite rate of increase (λ), net reproductive rates (R0), and mean generation time (T). The total preadult development time was 9.47 days for Ae. albopictus and 8.76 days for Ae. aegypti. The life expectancy was 19.01 days for Ae. albopictus and 19.94 days for Ae. aegypti. Mortality occurred mostly during the adult stage. The mean development time for each stage insignificantly correlated with temperature for Ae. albopictus (r = -0.208, P > 0.05) and (r = -0.312, P > 0.05) for Ae. aegypti. The population parameters suggest that Ae. albopictus and Ae. aegypti populations are r-strategists characterized by a high r, a large R0, and short T. This present study provides the first report to compare the life parameters of Ae. albopictus and Ae. aegypti strains from Penang island, Malaysia.

Matched MeSH terms: Laboratories
Fulltext Quisy-SDS accelerates the searching process of chemical safety datasheet

Muhammad Firdaus Othman,, Nor Aimi Abdul Wahab, Suhaiza Hasan, Marina Mokhtar, Noorezal Atfyinna Mohd Napiah, Mohd Noor Mokhtar, et al.

Jurnal Inovasi Malaysia, 2019;2(2):123-139.
MyJurnal

Teaching, learning and reseach activities in chemical laboratory usually involves a variety of hazardous chemicals. All chemicals stored in the laboratories should be accompanied by a safety data sheet which contains information such as chemical composition, safety precautions for handling and storing chemicals and emergency measures in case of accident. Ineffective management of chemical safety data sheet makes it difficult to access and resulting in slow action taken in the event of accident. To overcome this problem, Quisy-SDS was introduced to ensure a more effective chemical safety information management. Quisy-SDS provides a convenient method for all lab users and emergency respondents to acess the chemical safety information. This method uses a Quick Response (QR) code and website that allow users to acess the information in no time, anytime and anywhere. The use of Quisy-SDS has successfully reduced the search time of chemical safety information by 98% compared to the previous method.

Matched MeSH terms: Laboratories
Fulltext Bacterial identification process using BactFinder© mobile application

Nadiawati Alias, Nor Hasima Mahmod, Noor Afiza Badaluddin, Mohammad Khairul Asyraf Ridzuan

Jurnal Inovasi Malaysia, 2019;2(2):1-18.
MyJurnal

BactFinder© is an interactive and mobile application built to facilitate bacteria identification. As widely known, bacteria are composed of large domains of prokaryotic microorganisms which are various in types, hence the identification of these bacterial groups requires a systematic and orderly identification process. Through this mobile app, experimental results from biochemical and bacterial morphological tests carried out in the laboratory before hand must be submitted to the application before bacterial identification can be performed. Previously, academics user and students have to refer to many references such as research books and related academic journals in order to finalise their bacteria identification. This process, of course, will take relatively longer time and less effective. This BactFinder© mobile application is equipped with a database of 19 types of biochemical tests including bacteria morphology. The database was built based on Bergey’s Manual of Systematic Bacteriology, Bergey’s Manual of Determinative Bacteriology and other related scientific journals. The BactFinder© mobile app has been proved able to provide fast (± 2 seconds) and precise responses in helping students and academics user in the process of identifying bacteria species in the lab. This application is also suitable for undergraduate, post-graduate and academicians in the field of microbiology, biotechnology and science in general.

Matched MeSH terms: Laboratories
Genetics of glucose phosphate isomerase and phosphoglucomutase in Aedes albopictus (diptera: culicidae)

Yong HS, Chan KL, Dhaliwal SS, Cheong WH, Chiang GL, Mak JW

Theor Appl Genet, 1981 Nov;59(6):345-8.
PMID: 24276567 DOI: 10.1007/BF00276447

Glucose phosphate isomerase (E.C. 5.3.1.9) and phosphoglucomutase (E.C. 2.7.5.1) were found to be polymorphic in a laboratory colony of Aedes albopictus. The glucose phosphate isomerase locus is represented by two alleles resulting in three genotypes, while the phosphoglucomutase locus is represented by at least five alleles giving rise to a total of 15 genotypes. The inheritance of these two enzymes is of the Mendelian type with codominant alleles. Present data indicate that these genes are not linked.Of 105 mosquitoes analysed for these two gene-enzyme systems, the frequencies for glucose phosphate isomerase alleles are Gpi (S)=0.68 and Gpi (F)=0.32, while the frequencies for phosphoglucomutase alleles are Pgm (A)=0.16, Pgm (B)=0.11, Pgm (C)=0.19, Pgm (D)=0.30 and Pgm (F)= 0.24. The frequencies of the three glucose phosphate isomerase genotypes are in accord with Hardy-Weinberg expectations (X 1 (2) =2.74). Similarly, the frequencies of the 15 phosphoglucomutase genotypes probably do not differ significantly from Hardy-Weinberg expectations (X 10 (2) = 18.45).

Matched MeSH terms: Laboratories
Fulltext Review of knowledge, attitude, and practice among laboratory workers towards occupational safety and health

Nayef Shabbab Almutairi, Shamsul Bahri Bin Md Tamrin, Ng Yee Guan, Vivien How

Malaysian Journal of Medicine and Health Sciences, 2020;16(1):297-303.
MyJurnal

Laboratory workers exposed to diverse occupational exposures to accidents. Evidently, extant, yet very limited em- pirical underpinnings suggest that knowledge, practices and awareness of safety precautions are uncharacteristically poor among laboratory workers. As such, their demonstration of safety-related attitudes and practices have remained questionable. This paper, thus presents a systematic search of the literature on laboratory workers’ safety-related knowledge, attitudes, and practices. Specifically, literature published between 2007 and 2017 that characteristically attempted to evaluate knowledge, attitudes and practices among laboratory workers on Occupational Safety and Health were reviewed. Evidently, results from the review indicate poor knowledge, attitude and practice among the laboratory workers. It is critical that strategies be put in place by the management of the health facilities to institute and undertake activities in the form of training, improved safety management practices, organizational commitment and improved safety culture.

Matched MeSH terms: Laboratories
Fulltext Misleading free thyroxine (FT4): a case report

Nurul Hidayah Hashim, Intan Nureslyna Samsudin, Ana Dalila Masiman, Subashini C. Thambiah

Malaysian Journal of Medicine and Health Sciences, 2020;16(2):320-322.
MyJurnal

Interpretation of thyroid function test (TFT) is often straightforward but in certain scenarios, discordance between the clinical impression and the laboratory results exists. A 50-year-old woman with a ten years history of hypothyroidism on levothyroxine presented with a recent notable change in TFT [elevated free thyroxine (FT4) and thyroid-stimulat- ing hormone (TSH)], in an otherwise clinically euthyroid and previously stable TFT, leading to levothyroxine being withheld. This case report highlights the possibility of assay interference as a cause of discordant TFT. It also draws the importance of close collaboration between clinicians and the laboratory to avoid unnecessary investigations and inappropriate management of such a case.

Matched MeSH terms: Laboratories
Fulltext Improvement of urgent tests laboratory turnaround time through laboratory lean management

Salbiah Isa, Rohayu Hami, Hanita Hashim, Mohd Nizam S., Harani MS., Sairi S., et al.

Malaysian Journal of Medicine and Health Sciences, 2020;16(102):15-21.
MyJurnal

Introduction: Laboratory turnaround time (LTAT) is considered a reliable indicator of the quality and efficiency of a laboratory’s service. LTAT achievement, particularly of urgent tests, remains unsatisfactory and challenging in many clinical laboratories especially in tertiary health care centres with high workload and restricted resources. The unresolved issue of unsatisfactory urgent renal profile (RP) LTAT below the standard performance goal prompted our interest to improve laboratory’s handling of urgent test request. We thus implemented the Lean principle in the management of urgent test requests using urgent RP as the test model. Methods: The implementation of laboratory Lean involved 4 steps process; (1) Development of burning platform for change (2) Identification of waste (3) Plan- ning and implementation of control measures (4) Measuring, monitoring, and sustaining the improvement. Urgent RP LTAT and the percentage of the request met the time requirement determined based on the data extracted from laboratory information system (LIS) before and after the implementation of Lean was compared to assess the effec- tiveness. Results: Urgent RP LTAT after the implementation of Lean was reduced i.e 35 min (before) vs 31 min (after), with the percentage of LTAT met the time requirement was significantly increased above the set target i.e 82.8% (before) to 93.5% (after) with P-value = 0.001. Conclusion: Implementation of innovation using Lean management has significantly improved urgent RP LTAT achievement, thus optimised urgent test management in our Chemical Pathology laboratory. Lean is a strongly recommended strategy to improve urgent test LTAT especially in laboratories with restricted resources.

Matched MeSH terms: Laboratories
Fulltext Introducing a technique in sustaining adult worker bees of Heterotrigona itama (Cockerell, 1918) in laboratory to facilitate future health research on Kelulut honey

Syazana Jumaan, Jahangir Kamaldin, Rosliza Jajuli, Izfa Riza Hazmi

Malaysian Journal of Medicine and Health Sciences, 2020;16(102):22-30.
MyJurnal

Introduction: The study is introducing a laboratory technique to sustain the longevity of Heterotrigona itama sting- less bees collected from the farm in order to facilitate future health research on Kelulut honey. Methods: The worker bees were held in laboratory at 26 ± 2 oC, 57 ± 8 % relative humidity (RH) and 12:12 hours (light:dark period) in a cup covered with meshed cloth and installed with an inverted-wick system consists of a drinking straw with the bottom end loosely plugged with cotton wool. The artificial diet was pipetted into the straw to wet the cotton wool. The bees were divided into five diet groups, namely a) unfed - control, b) distilled water, c) purified tap water, d) non-carbonated isotonic drink or e) 5% honey solution. Feeding activity and survival of worker bees were observed daily. Results: The worker bees are seen to frequent and lick the cotton wool wetted with artificial diets. Comparison between the artificial diets, Kaplan-Meier statistical analysis showed that the 5% honey solution and non-carbonated isotonic drink have significantly (P < 0.05) extended the longevity of the worker bees with 50% survival probability at least 8 days. When the similar holding and feeding technique used for the bees from commercial farms, the 50% survival probability was extended to 14 days. Conclusion: The inverted-wick system with the use of 5% honey solu- tion or isotonic drink as the artificial diet is capable to hold the H. itama worker bees at least for a week with survival above 50% for laboratory experiments.

Matched MeSH terms: Laboratories
Fulltext Bioaerosol levels in indoor air of animal house and hospital laboratories; a comparison with library and administrative offices

Siti Marwanis Anua, Nur Fatin Haris, Nurzafirah Mazlan

Malaysian Journal of Medicine and Health Sciences, 2020;16(101):94-100.
MyJurnal

Introduction: This study reported the concentration of bacterial and fungal bioaerosol at an animal house and hospi- tal laboratories with the aim to compare the concentration levels at library and administrative offices. The bioaerosol levels between mid-shift (afternoon) were also compared to the concentration measured during pre-shift (morning). Methods: The NIOSH 0800 method utilising microbiological air sampler collecting airborne bacterial and fungal samples via impaction technique on Nutrient agar (NA) and Sabouraud Dextrouse agar (SDA) as culture medium, respectively. Sampling was done twice daily; before (pre-shift) and during working (mid-shift) hour. Results: The highest bacteria and fungi concentration was recorded at the animal house with median concentration of 2477 CFU/ m3 (IQR=121-2477) and 791 CFU/m3 (IQR = 379-2081), respectively. Higher-risked workplaces such as animal house and hospital laboratories have significantly higher bioaerosol concentrations compared to control workplaces such as library and administrative offices (p

Matched MeSH terms: Laboratories, Hospital
Replicating remembering "remembering"

Janssen SMJ, Anthony K, Chang CYM, Choong EL, Neoh JY, Lim A

Memory, 2020 Dec 31.
PMID: 33382346 DOI: 10.1080/09658211.2020.1868525

When examining spontaneously recovered memories of childhood sexual abuse, victims report that there had been periods in which they had forgotten the abuse. However, there are sometimes people with whom the victim had spoken about the abuse during the period in which the victim had supposedly forgotten the abuse, suggesting the victim had not forgotten the abuse but the prior recall of the abuse. The underestimation of previous knowledge is termed the forgot-it-all-along effect. The goal of the present study was replicating the results of a laboratory study that had provided a theoretical understanding for the forgot-it-all-along effect by showing that people have difficulties remembering "remembering" when the memory had previously been recalled in a different context. The effect was replicated by using the same neutral context sentences, suggesting the finding was robust. We also extended the experimental design by using positive and negative context sentences, but it did not become smaller when the positive sentences provided the different context or larger when the negative sentences provided the different context. Although the sample sizes were sufficiently large to provide statistical power for the forgot-it-all-along effect, they may not have been sufficiently large to observe the moderation effects of emotional context.

Matched MeSH terms: Laboratories
Qualitative and Quantitative Analysis of Mycotoxins

Rahmani A, Jinap S, Soleimany F

Compr Rev Food Sci Food Saf, 2009 Jul;8(3):202-251.
PMID: 33467794 DOI: 10.1111/j.1541-4337.2009.00079.x

Mycotoxin toxicity occurs at very low concentrations, therefore sensitive and reliable methods for their detection are required. Consequently, sampling and analysis of mycotoxins is of critical importance because failure to achieve a suitable verified analysis can lead to unacceptable consignments being accepted or satisfactory shipments unnecessarily rejected. The general mycotoxin analyses carried out in laboratories are still based on physicochemical methods, which are continually improved. Further research in mycotoxin analysis has been established in such techniques as screening methods with TLC, GC, HPLC, and LC-MS. In some areas of mycotoxin method development, immunoaffinity columns and multifunctional columns are good choices as cleanup methods. They are appropriate to displace conventional liquid-liquid partitioning or column chromatography cleanup. On the other hand, the need for rapid yes/no decisions for exported or imported products has led to a number of new screening methods, mainly, rapid and easy-to-use test kits based on immuno-analytical principles. In view of the fact that analytical methods for detecting mycotoxins have become more prevalent, sensitive, and specific, surveillance of foods for mycotoxin contamination has become more commonplace. Reliability of methods and well-defined performance characteristics are essential for method validation. This article covers some of the latest activities and progress in qualitative and quantitative mycotoxin analysis.

Matched MeSH terms: Laboratories
Fulltext Surface-Mounted Bare and Packaged Fiber Bragg Grating Sensors for Measuring Rock Strain in Uniaxial Testing

Isah BW, Mohamad H

Sensors (Basel), 2021 Apr 22;21(9).
PMID: 33922008 DOI: 10.3390/s21092926

The paper explores the possibility of using high-resolution fiber Bragg grating (FBG) sensing technology for on-specimen strain measurement in the laboratory. The approach provides a means to assess the surface deformation of the specimen, both the axial and radial, through a chain of FBG sensor (C-FBG), in a basic setup of a uniaxial compression test. The method is cost-effective, straightforward and can be commercialized. Two C-FBG; one was applied directly to the sample (FBGBare), and the other was packaged (FBGPack) for ease of application. The approach measures the local strain with high-resolution and accuracy levels that match up to the existing local strain measuring sensors. The approach enables the evaluation of small-strain properties of the specimen intelligently. The finite element model analysis deployed has proven the adaptability of the technique for measuring material deformation. The adhesive thickness and packaging technique have been shown to influence the sensitivity of the FBG sensors. Owing to the relative ease and low-cost of instrumentation, the suggested method has a great potential to be routinely applied for elemental testing in the laboratory.

Matched MeSH terms: Laboratories
Potent allelopathy and non-PSTs, non-spirolides toxicity of the dinoflagellate Alexandrium leei to phytoplankton, finfish and zooplankton observed from laboratory bioassays

Shang L, Xu Y, Leaw CP, Lim PT, Wang J, Chen J, et al.

Sci Total Environ, 2021 Aug 01;780:146484.
PMID: 33774286 DOI: 10.1016/j.scitotenv.2021.146484

The dinoflagellate genus Alexandrium has been well known for causing paralytic shellfish poisoning (PSP) worldwide. Several non-PSP toxin-producing species, however, have shown to exhibit fish-killing toxicity. Here, we report the allelopathic activity of Alexandrium leei from Malaysia to other algal species, and its toxicity to finfish and zooplankton, via laboratory bioassays. Thirteen microalgal species that co-cultured with Al. leei revealed large variability in the allelopathic effects of Al. leei on the test algae, with the growth inhibition rates ranging from 0 to 100%. The negative allelopathic effects of Al. leei on microalgae included loss of flagella and thus the motility, damages of chain structure, deformation in cell morphology, and eventually cell lysis. The finfish experienced 100% mortality within 24 h exposed to the live culture (2000-6710 cells·mL-1), while the rotifer and brine shrimp exhibited 96-100% and 90-100% mortalities within 48 h when exposed to 500-6000 cells·mL-1 of Al. leei. The mortality of the test animals depended on the Al. leei cell density exposed, leading to a linear relationship between mortality and cell density for the finfish, and a logarithmic relationship for the two zooplankters. When exposed to the treatments using Al. leei whole live culture, cell-free culture medium, extract of algal cells in the f/2-Si medium, extract of methanol, and the re-suspended freeze-and-thaw algal cells, the test organisms (Ak. sanguinea and rotifers) all died at the cell density of 8100 cells·mL-1 within 24 h. Toxin analyses by HILIC-ESI-TOF/MS and LC-ESI-MS/MS demonstrated that Al. leei did not produce PSP-toxins and 13-desmethyl spirolide C. Overall, our findings demonstrated potent allelopathy and toxicity of Al. leei, which do not only pose threats to the aquaculture industry, fisheries, and marine ecosystems but may also play a part role in the population dynamics and bloom formation of this species.

Matched MeSH terms: Laboratories
Fulltext Impact of diabetes mellitus on clinico-laboratory characteristics and in-hospital clinical outcomes among patients with myocardial infarction

Khalid SH, Liaqat I, Mallhi TH, Khan AH, Ahmad J, Khan YH

J Pak Med Assoc, 2020 Dec;70(12(B)):2376-2382.
PMID: 33475547 DOI: 10.47391/JPMA.370

OBJECTIVE: Diabetes mellitus (DM) along with myocardial infarction (MI) carries increased burden on patients in terms of morbidity, mortality and cost. Current study was aimed to investigate the impact of DM on clinico-laboratory characteristics on in-hospital treatment outcomes among MI patients.o compare the outcome of mesh hernioplasty performed under local anaesthesia in relatively young and older patients regarding wound complications and urinary retention.
METHODS: All MI patients admitted to the emergency department of Faisalabad Institute of Cardiology from April, 2016 to March, 2017 were recruited into the study. The clinico-laboratory profile and in-hospital outcomes of patients with and without DM were compared using chi-squared test or student t-test, where appropriate.
RESULTS: A total 4063 patients (Mean age: 55.86 ± 12.37years) with male preponderance were included into the study. STEMI was most prevalent (n = 2723, 67%) type of MI among study participants. DM was present in substantial number of cases (n = 3688, 90.8%). Patients with DM presented with increased BMI, higher blood pressure, elevated levels of cholesterol, serum creatinine, and blood urea nitrogen, when compared to the patients without DM (p<0.05). Out of 560 patients who were followed up, cardiogenic shock was frequent (n = 293, 52.3%) adverse outcome followed by heart failure (n = 114, 20.4%), atrial fibrillation (n = 78, 13.9%) and stroke (n = 75, 13.4 %). Moreover, in-hospital adverse outcomes were more prevalent among MI patients with DM than those without DM.
CONCLUSIONS: MI patients with DM present with varying clinico laboratory characteristics as well as experience higher prevalence of adverse cardiovascular events as compared to patients without DM. These patients require individual management strategy on very first day of admission.

Matched MeSH terms: Laboratories
A new species of cockroach, Periplaneta gajajimana sp. nov., collected in Gajajima, Kagoshima Prefecture, Japan

Komatsu N, Lio H, Ooi HK

Trop Biomed, 2021 Jun 01;38(2):48-52.
PMID: 33973572 DOI: 10.47665/tb.38.2.036

We described a new species of cockroach, Periplaneta gajajimana sp. nov., which was collected in Gajajima, Kagoshima-gun Toshimamura, Kagoshima Prefecture, Japan, on November 2012. The new species is characterized by its reddish brown to blackish brown body, smooth surface pronotum, well developed compound eyes, dark brown head apex, dark reddish brown front face and small white ocelli connected to the antennal sockets. In male, the tegmen tip reach the abdomen end or are slightly shorter, while in the female, it does not reach the abdominal end and exposes the abdomen beyond the 7th abdominal plate. We confirmed the validity of this new species by breeding the specimens in our laboratory to demonstrate that the features of the progeny were maintained for several generations. For comparison and easy identification of this new species, the key to species identification of the genus Periplaneta that had been reported in Japan to date are also presented.

Matched MeSH terms: Laboratories
Fulltext Delivery time of orthodontic appliances from government dental laboratories in Selangor

Loke, S.T., Tan, S.Y.

Malaysian Dental Journal, 2011;32(1):5-11.
MyJurnal

Compliance to delivery of orthodontic appliances within specified times and factors which influenced compliance by dental technicians in Selangor was evaluated. This is a prospective 8-month study of 18 trainee/ trained technicians from 4 main government dental laboratories in Kajang, Klang, Tanjong Karang and Shah Alam. Delivery times specified by orthodontists were 1 day for plastic retainer, 3 days acrylic retainer, 5 days active plates (URA), 10 days functional appliances (FA), 10 days transpalatal arch (TPA) and 10 days for quadhelix. Punctual delivery was recorded as ‘compliant’. Compliance was compared between appliances, clinics, technicians, orthodontists and seniority of technicians. The sample comprised appliances from 365 patients; 38 (10.4%) Tanjong.Karang, 114 (31.2%) Kajang, 191 (52.3%) Klang and 22 (6.0%) Shah Alam. The majority of appliances were retainers (66.3%), followed by URA (13.4%), functionals (9.3%), TPA (9.0%) and quadhelix (1.9%). Mean compliance for all appliances in Selangor was 55%. Plastic retainers had highest compliance (77.8%), followed by acrylic retainers (59.9%), quadhelix (57.1%), FA (47.1%), TPA (45.5%) and URA (24.5%). Senior technicians (>3 years in service) were more compliant than juniors (

Matched MeSH terms: Laboratories, Dental
Fulltext GIS based assessment on landslide hazard zonation: case study of Cameron Highlands – Gua Musang road Kelantan, Malaysia

Mohamed M. GahGah, Juhari Mat Akhir, Abdul Ghani M. Rafek, Ibrahim Abdullah

Sains Malaysiana, 2009;38(6):827-833.

The aim of this study is to investigate the factors that cause landslides in the area along the new road between Cameron Highlands and Gua Musang. Landslide factors such as lineaments have been extracted from remote sensing data (Landsat TM image) using ERDAS software. A soil map has been produced using field work and laboratory analysis, and the lithology, roads, drainage pattern and rainfall have been digitized using ILWIS software together with the slope angle and elevation from the Digital Elevation Model (DEM). All these parameters, which are vital for landslide hazard assessment, have been integrated into the geographical information system (GIS) for further data processing. Weightage for these landslide relevant factors related to their influence in landslide occurrence using the heuristic method has been carried out. The results from this combination through a modified ‘index overlay with multi class maps’ model was used to produce a landslide hazard zonation map. Five classes of potential landslide hazard have been derived as the following: very low hazard zone 17.27%, low hazard zone 39.35%, medium hazard zone 25.1%, high hazard zone 15.35% and very high hazard zone 2.93%. The results from this work have been checked through the landslide inventory using available aerial photos interpretation and field work, and show that the slope and elevation have the most direct affect on landslide occurrence.

Matched MeSH terms: Laboratories
Fulltext Hemolyzed Specimens: Major Challenge for Identifying and Rejecting Specimens in Clinical Laboratories

Wan Azman WN, Omar J, Koon TS, Tuan Ismail TS

Oman Med J, 2019 Mar;34(2):94-98.
PMID: 30918601 DOI: 10.5001/omj.2019.19

Pre-analytical quality in clinical chemistry testing is as important as analytical and post-analytical quality. The most prevalent pre-analytical interference and a major source of error producing unreliable laboratory test results is hemolysis of blood samples. In vitro hemolysis may be due to the blood withdrawal technique or sample handling whereas in vivo hemolysis can originate from acquired, hereditary, or iatrogenic conditions and is not technique dependent. Interpreting in vivo or in vitro hemolysis requires clinicians to supply reliable clinical history and findings. Even then, to reject or release the result with interpretation is still under debate. Thus, hemolyzed specimens are a serious pre-analytical problem calling for well-designed and strictly implemented laboratory guidelines. The aim of this non-systematic review (addressed to healthcare professionals) was to highlight the challenges in identifying and rejecting hemolysis specimens.

Matched MeSH terms: Laboratories
Prevention of Group A streptococcal infection in Malaysia

Jamal F

Family Practitioner, 1988;11(1):73-74.

Group A streptococcus is an important cause of pharyngitis, skin infection and or its non-suppurative sequelae, i.e. rheumatic fever and post-streptococcal glomerulonephritis. The National Streptococcus Reference Laboratory was established in 1982 to collect data on various aspects of this infection.

Matched MeSH terms: Laboratories
Fulltext Transformation improvement with the Standardized Pressure Agrobacterium Infiltration Device (SPAID)

Mad' Atari MFB, Folta KM

BMC Res Notes, 2019 Mar 15;12(1):144.
PMID: 30876440 DOI: 10.1186/s13104-019-4117-3

OBJECTIVE: The treatment of plant tissue with Agrobacterium tumefaciens is often a critical first step to both stable and transient plant transformation. In both applications bacterial suspensions are oftentimes physically introduced into plant tissues using hand-driven pressure from a needleless syringe. While effective, this approach has several drawbacks that limit reproducibility. Pressure must be provided with the syringe perfectly perpendicular to the tissue surface. The researcher must also attempt to provide even and consistent pressure, both within and between experimental replicates. These factors mean that the procedures do not always translate well between research groups or biological replicates.
RESULTS: We have devised a method to introduce Agrobacterium suspensions into plant leaves with greater reproducibility. Using a decommissioned dissecting microscope as an armature, a syringe body with the bacterial suspension is mounted to the nosepiece. Gentle, even pressure is applied by rotating the focus knob. The treatment force is measured using a basic kitchen scale. The development of the Standardized Pressure Agrobacterium Infiltration Device (SPAID) provides a means to deliver consistent amounts of bacterial suspensions into plant tissues with the goal of increasing reproducibility between replicates and laboratories.

Matched MeSH terms: Laboratories

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