Displaying publications 141 - 160 of 254 in total

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  1. Khalid AA, Abd Razak NI, Anuar ST, Ibrahim YS, Rusli MU, Jaafar M
    Mar Pollut Bull, 2024 Dec 13;211:117412.
    PMID: 39674038 DOI: 10.1016/j.marpolbul.2024.117412
    This study presents the first evidence of microplastics in natural sea turtle nests at Chagar Hutang Turtle Sanctuary (CHTS) on Redang Island, a crucial habitat for green turtles. Microplastics were detected in all studied turtle nests (0-70 cm depth), with a total abundance of 12,270 microplastic items per kg dry weight of sand. Fibers (80.7 %) were the most prevalent shape, primarily black (37.9 %), with a high proportion of small-sized particles (<300-1000 μm), indicating prolonged accumulation and environmental degradation. These microplastics, primarily polyethylene terephthalate (PET) and polyethylene (PE), are likely from fishing nets, food packaging, and textiles. Despite the sanctuary's remote location, it is heavily impacted by microplastics carried by tides and wind, raising concerns about their potential effects on turtle egg incubation. The findings offer critical insights into the impact of microplastics on turtle habitats and underscore the urgent need for conservation efforts to protect green sea turtles in Malaysia.
  2. Saddki N, Noor MM, Norbanee TH, Rusli MA, Norzila Z, Zaharah S, et al.
    AIDS Care, 2009 Oct;21(10):1271-8.
    PMID: 20024703 DOI: 10.1080/09540120902803216
    This study determines the validity and reliability of the Malay version of the World Health Organization Quality of Life (WHOQOL) assessment instrument in patients with human immunodeficiency virus (HIV) infection. A cross-sectional study on 157 patients with HIV seen at the Infectious Disease Unit, Hospital Raja Perempuan Zainab II, Kota Bharu, Kelantan was conducted. Factor analysis identified five major domains: physical needs, spirituality, social relationship, psychological, and environment. Significant correlation was found between each domain scores and the general health questions. The instrument was able to discriminate between asymptomatic and symptomatic HIV positive patients for all domain scores except for the spirituality domain. The internal consistency of the five domains ranged from 0.70 to 0.83. The intraclass correlation coefficient (ICC) ranged from 0.60 to 0.87 across all domains. In conclusion, the Malay version of WHOQOL-HIV BREF is a valid and reliable instrument in assessing quality of life in HIV positive patients.
  3. Chung CM, Lu MZ, Wong CY, Goh SG, Azhar MI, Lim YM, et al.
    Diabet Med, 2016 May;33(5):674-80.
    PMID: 26202696 DOI: 10.1111/dme.12864
    AIM: The aim of this study is to construct a new tool for the assessment of sexual dysfunction among men with diabetes that is valid and reliable across different ethnicities, languages and socio-economic backgrounds in South East Asia.

    METHODS: Focus group interviews were conducted to determine the construct of the questionnaire. Content and face validity were assessed by a panel of experts. A pilot study was conducted to validate the Sexual Dysfunction in Asian Men with Diabetes (SAD-MEN) questionnaire in English and Malay. The International Index of Erectile Function-5 (IIEF-5) was used for comparison. Construct validity was assessed using exploratory factor analysis, reliability was determined using Cronbach's α (> 0.700), and test-retest reliability using Spearman's rank correlation coefficient.

    RESULTS: The SAD-MEN questionnaire yielded moderate face and content validity, with high reliability as shown by Cronbach's α values of 0.949 for sexual performance and 0.775 for sexual desire for the English version. The Malay language questionnaire had a Cronbach's α value of 0.945 for sexual performance and 0.750 for sexual desire. Test-retest reliability using Spearman's test gave correlation coefficients of r = 0.853, P = 0.000 for the English language questionnaire and r = 0.908, P = 0.000 for the Malay language questionnaire.

    CONCLUSION: The SAD-MEN questionnaire is a valid and reliable tool by which to assess sexual dysfunction in English- and Malay-speaking Malaysian and South East Asian men with diabetes.

  4. Md Pauzi SH, Saari HN, Roslan MR, Syed Khair Azman Jamalulil SNS, Tauan IS, Mohd Rusli FA, et al.
    Malays J Pathol, 2019 Aug;41(2):133-138.
    PMID: 31427548
    INTRODUCTION: Evaluation of HER2 status in breast cancer using immunohistochemistry (IHC) and in-situ-hybridisation (ISH) study is important to establish prognosis and to select patient for targeted therapy.

    OBJECTIVE: The study aims to determine the concordance between HER2 protein IHC score and its gene status by dual-colour dual-hapten in-situ-hybridization (DDISH) study.

    MATERIALS AND METHODS: Retrospective study was performed on 767 referred breast cancer cases over a period of five years. The HER2 IHC score (the initial and repeat test score) and the results of HER2 gene status by DDISH were retrieved from the histopathological reports. The agreement between initial IHC score with repeat test score was measured using Cohen Kappa. Chi square test analyzed the association between HER2 IHC score with its gene status by DDISH.

    RESULTS: The concordance of HER2 IHC score between the initial and repeat test were 52.7% and 89.4% for IHC score 2+ and 3+ respectively. There was moderate agreement of HER2 IHC score between the initial and repeat test score (ϰ = 0.526, p<0.001). A significant association noted between HER2 IHC score with its gene status by DDISH (p<0.001). Only 56 out of 207 cases (27.1%) with 2+ IHC score showed HER2 gene amplification while the majority of cases with 3+ IHC score were gene-amplified (446 out of 451, 98.9%).

    CONCLUSION: ISH study should be done in all IHC-equivocal cases (2+) to select patient for targeted therapy. Gene amplification must also be confirmed in IHC-positive cases (3+) to prevent from giving non-effective treatment with possible adverse effects to patient with non-amplified HER2 gene.

  5. Md Said R, Mohd Zain R, Chan HK, Soelar SA, Rusli N, Nasir NH, et al.
    J Viral Hepat, 2020 06;27(6):638-643.
    PMID: 31997563 DOI: 10.1111/jvh.13267
    Approximately 2.5% of the Malaysian population is currently living with hepatitis C virus (HCV) infection. Yet, the public awareness of the disease is limited and under-screening remains a major challenge. With the support of international non-for-profit organizations, the Ministry of Health in Malaysia recently launched a one-week nationwide hepatitis C screening campaign in conjunction with the World Hepatitis Day. For the first time, the rapid diagnostic test (RDT) for HCV screening was introduced in public health institutions. This campaign involved 49 hospitals and 38 health clinics across the country, targeting the adult general population with unknown HCV infection status. Of the 11 382 participants undergoing the RDT, 1.9% were found to be positive for hepatitis C antibody (anti-HCV) and were referred to on-site medical departments or nearby hospitals for confirmatory testing and treatment. Men, the Malay ethnic group, intranasal and injection drug users and ex-prisoners were shown to have higher odds of being positive for anti-HCV. In addition to serving as a model to educate the general population about the disease, this campaign demonstrates the feasibility of decentralizing HCV screening, particularly by promoting the use of RDT, and linking the HCV-infected patients to care in Malaysia.
  6. Chuan MW, Wong KL, Riyadi MA, Hamzah A, Rusli S, Alias NE, et al.
    PLoS One, 2021;16(6):e0253289.
    PMID: 34125874 DOI: 10.1371/journal.pone.0253289
    Silicene has attracted remarkable attention in the semiconductor research community due to its silicon (Si) nature. It is predicted as one of the most promising candidates for the next generation nanoelectronic devices. In this paper, an efficient non-iterative technique is employed to create the SPICE models for p-type and n-type uniformly doped silicene field-effect transistors (FETs). The current-voltage characteristics show that the proposed silicene FET models exhibit high on-to-off current ratio under ballistic transport. In order to obtain practical digital logic timing diagrams, a parasitic load capacitance, which is dependent on the interconnect length, is attached at the output terminal of the logic circuits. Furthermore, the key circuit performance metrics, including the propagation delay, average power, power-delay product and energy-delay product of the proposed silicene-based logic gates are extracted and benchmarked with published results. The effects of the interconnect length to the propagation delay and average power are also investigated. The results of this work further envisage the uniformly doped silicene as a promising candidate for future nanoelectronic applications.
  7. Rusli SM, Choo JM, Lee TH, Piozzi GN, Cuellar-Gomez H, Baek SJ, et al.
    Colorectal Dis, 2023 Mar;25(3):431-442.
    PMID: 36281503 DOI: 10.1111/codi.16387
    AIM: The applicability of laparoscopic D3 oncological resection for splenic flexure cancer (SFC) surgery has not been fully explored due to technical difficulties and variations in surgical procedure. The aim of this work is to describe the feasibility of performing laparoscopic D3 resection in SFC and its impact on long-term survival.

    METHOD: A retrospective study on 47 out of 52 consecutive patients who underwent elective laparoscopic colectomy for SFC from December 2006 until December 2019 at Korea University Anam Hospital was performed. Data on patients' demographic and clinical features, surgical procedures, intraoperative and postoperative complications, pathological features and follow-up were collected. Categorical data are expressed as frequencies (n) and percentages (%). Continuous data are expressed as mean ± standard deviation and median (range). The Kaplan-Meier test was used to determine the overall survival (OS), progression-free survival (PFS) and disease-free survival (DFS).

    RESULTS: The median age of patients was 67.0 years (range 27-87 years) and 72.3% were men. Ten (21.3%) patients presented with an obstructing tumour and underwent an elective laparoscopic colectomy, while 68.1% of patients presented with Stage II and III disease. The conversion rate was 4.3% and the morbidity rate was 31.9%. There was one postoperative death secondary to splenic infarction and anastomotic leak leading to multi-organ failure. Four deaths occurred due to disease progression during a median follow-up of 63.8 months. The rate of recurrence was 20%, the 5-year OS was 89.6% and the 5-year PFS was 72.9%. After R0 resection, the 5-year OS was 91.5% and the 5-year DFS was 74.5%.

    CONCLUSION: Laparoscopic D3 colectomy for SFC is feasible, with an acceptable morbidity and long-term oncological outcome when performed by highly skilled laparoscopic colorectal surgeons with knowledge of the complex anatomy around the splenic flexure. Further randomized trials should be performed to determine the advantage of laparoscopic D3 colectomy over conventional colectomy for SFC.

  8. Hashim NM, Che Daud AZ, Ibrahim AH, Ab Majid MH, Mohd Ghazali MN, Abdul Razak MM, et al.
    Prosthet Orthot Int, 2024 Jan 01;48(1):89-99.
    PMID: 37639558 DOI: 10.1097/PXR.0000000000000270
    BACKGROUND: An excellent validated and reliable instrument is paramount in holistically evaluating the prosthetic and orthotic (P&O) service, encompassing functional outcomes, health-related quality of life (HRQoL), and patient satisfaction with devices and service.

    OBJECTIVE: To perform a translation and cross-cultural adaptation of 3 modules of the Orthotics and Prosthetics Users' Survey (OPUS): (1) lower-extremity functional status (LEFS), (2) client satisfaction with device and services (CSDS), and (3) HRQoL in Malay language, and analyze its psychometric properties.

    STUDY DESIGN: Translation and validation study.

    METHODS: This translation process consisted of 4 phases: (1) a forward-backward translation, (2) content and face validity by utilizing content and face validity indices, (3) pilot testing and psychometric analysis using exploratory factor analysis, and (4) test-retest reliability.

    RESULTS: One item from OPUS Health Quality of Life Index-Malay pilot version, 5 items from OPUS LEFS-Malay pilot version, and 4 items of OPUS Satisfaction with Device and Services-Malay pilot version were deleted because of poor factor loading of <0.6. The final version of Modified OPUS HRQoL-M, Modified OPUS LEFS-M, and Modified OPUS CSDS-M consisted of 22 items, 15 items, and 17 items, respectively. The final versions of all 3 Modified OPUS Malay version possess good internal consistency of 0.854, 0.927, and 0.98, and intraclass correlation of 0.773, 0.871, and 0.821, respectively .

    CONCLUSION: Modified OPUS HRQoL-M, Modified OPUS LEFS-M, and Modified OPUS CSDS-M are valid and reliable instruments to be adopted into the local Malaysia population.

  9. Amer Hamzah M, Mohd Kasim NA, Shamsuddin A, Mustafa N, Mohamad Rusli NI, Teh CY, et al.
    3 Biotech, 2020 Mar;10(3):105.
    PMID: 32099746 DOI: 10.1007/s13205-020-2092-y
    In this study, we analyzed the Rc and Rd genes that are responsible for the coloration of rice pericarps from six upland rice varieties. We also examined the association of pericarp coloration to the single nucleotide polymorphism in 9-cis-epoxycarotenoid dioxygenase 2 (NCED2), a key gene involved in abscisic acid (ABA) biosynthesis. Our findings demonstrated that all the upland rice varieties analyzed have a Rd gene which encodes a complete dihydroflavonol-4-reductase without early translational termination codon irrespective of their pericarp colors. However, the upland rice varieties with white pericarps were found to have a defective Rc gene with a 14-base deletion at exon 7 which could disrupt the function of a positive regulator of proanthocyanidin biosynthesis. In addition, the NCED2 genes from the upland rice varieties with white pericarps in this study have a C-allele while the NCED2 genes from Pandasan Red, Tomou and Taragang varieties that bear red pericarps were found to have a T-allele which was reported to be associated with a higher ABA level in upland rice. A better understanding of the gene sequences of upland rice varieties with red pericarp may provide important information for rice breeding programs.
  10. Azfar ZM, Nazri SM, Rusli AM, Maizurah O, Zahiruddin WM, Azwany YN, et al.
    J Prev Med Hyg, 2018 Mar;59(1):E92-E98.
    PMID: 29938244 DOI: 10.15167/2421-4248/jpmh2018.59.1.776
    INTRODUCTION: Many efforts have been done to reduce leptospirosis infections in Malaysia especially among high risk groups including town service workers. Town service workers are more likely to be exposed to the leptospiral infection resulting from their occupational activities.

    METHODS: A cross sectional study was conducted in northeastern Malaysia involving 321 town service workers who were subjected to answer an interviewer-guided validated questionnaire which consists of sociodemographic, knowledge, attitude and practice information. Data were entered and analyzed using SPSS Version 20.

    RESULTS: All of the respondents were Malay with mean (SD) age of 40.6 (10.28) years old. The mean (SD) duration of employment was 12.1 (9.62) years. Fifty four respondents (16.8%) had never heard of leptospirosis. Among the respondents, 215 (67.0%) of them had poor knowledge on leptospirosis. Meanwhile, 167 (52.0%) and only 128 (39.9%) of them had satisfactory attitude and practice respectively. It was found that knowledge on risk factors for leptospirosis was lacking. There were high risk attitudes such as drinking habit and protective equipment used during working with the favourable answers ranged from 67.3% to 89.1%. The weakest area identified in their practice was also on the use of protective equipment.

    CONCLUSIONS: The workers' level of knowledge and practice were relatively poor despite an overall good practice on leptospirosis. This finding might expose them to an increased risk of contracting leptospirosis. Identified weak areas in their knowledge, attitude and practice will assist the policy makers to develop a focused and well-directed intervention program on leptospirosis infection.

  11. Ismail A, Reffien MAM, Ibrahim NM, Sobri HNM, Abidin NDIZ, Rusli SASS, et al.
    Glob J Qual Saf Healthc, 2020 Nov;3(4):134-138.
    PMID: 37260578 DOI: 10.36401/JQSH-20-10
    INTRODUCTION: Globally, stroke continues to become a significant public health issue contributing to one of the significant causes of morbidity and mortality. The study aimed to describe the characteristics of patients with stroke who were admitted to a teaching hospital in Malaysia and to determine the factors associated with length of stay (LOS).

    METHODS: This is a single-center, cross-sectional study using in-patient data maintained by the Case-Mix Unit of a teaching hospital in Malaysia from 2016 to 2017. The study included all patients with International Classification of Disease (ICD) code 164 (stroke, not specified as hemorrhage or infarct). The significance of association was determined using nonparametric tests in the form of the Mann-Whitney U test and the Kruskal-Wallis test.

    RESULTS: A total of 162 stroke patients from 2016 to 2017 from Case-Mix database were included in the study. The age ranged from 31 to 97 years old. The minimum and maximum LOS for patients with stroke ranged from 1 to 17 days. The severity of illness was found to be significantly associated with longer LOS (p < 0.001); however, age, sex, and presence of co-morbidities did not show any significant association.

    CONCLUSION: Despite its limitations, this study is an essential first step to examine the characteristics of patients with stroke and to determine the factors associated with LOS.

  12. Yusoff NYM, Aris H, Nasiruddin WHM, Rusli AKC, Yurnaidi Z, Safrina R, et al.
    PMID: 39028458 DOI: 10.1007/s11356-024-34358-8
    Renewable energy consumption is a crucial solution to addressing pressing environmental issues, particularly climate change and air pollution. Investigating the factors that drive its adoption is highly significant, as it provides policymakers and stakeholders with valuable insights to accelerate the transition to renewable energy sources. Through this approach, we can minimise the negative consequences of our reliance on fossil fuels, thereby protecting the integrity of the environment. Therefore, the primary goal of this study is to thoroughly investigate the main factors that influence renewable energy consumption and environmental change in six specifically chosen ASEAN countries. The stationarity of the 1990-2019 data was tested using panel data techniques such as Levin, Lin, and Chu (LLC), Im Pesaran (IPS), and the Shin W-stat test. According to the stationarity tests, after the first order, all variables exhibit stationarity. Additionally, Pedroni's co-integration test result confirmed that there was a long-term relationship among the variables. Different methods, such as dynamic ordinary least squares (DOLS), fully modified ordinary least squares (FMOLS), and pooled ordinary least squares (POLS), are used for cointegration estimating. The results suggest that there is a positive co-integration between renewable energy use and GDP in six ASEAN countries, indicating a long-term relationship. The positive relationship between GDP and renewable energy use suggests that economic growth is the primary driving force behind ASEAN's renewable energy adoption. However, factors like carbon emissions, population density, and foreign direct investment (FDI) negatively impact the demand for renewable energy. The limited availability of renewable energy in certain ASEAN countries may discourage foreign direct investment (FDI) due to the inverse relationship between FDI and renewable energy use. The studies also revealed that carbon emissions, which contribute to environmental pollution, do not motivate industries to invest in renewable energy. This finding would challenge the Environmental Kuznets Curve (EKC) hypothesis. According to the EKC, there is a significant transition towards renewable energy as a response to environmental degradation. However, it is worth noting that several ASEAN countries have experienced economic growth while also experiencing higher levels of carbon emissions. Given that economic expansion might not be environmentally beneficial, this research has implications for ASEAN energy policies. The ASEAN region faces a challenge in investing in renewable energy due to the excessive dependence on fossil fuels. Therefore, an in-depth evaluation of the main factor behind ASEAN's environmental concerns, which promotes the adoption of renewable energy, can greatly influence policy decisions, particularly in attaining net zero emissions. Policymakers can utilise this comprehensive analysis to establish informed objectives for policies related to renewable energy and develop strategic plans, i.e. reforming fuel subsidies. The goal is to encourage the development of environmentally friendly and sustainable energy plans for the future in the ASEAN region.
  13. Al-Zurfi, Balsam Mahdi Nasir, Fuad, Maher D., Abdelqader, Mohammed A., Baobaid, Mohammed Faez, Elnajeh, Maged, Hasanain Faisal Ghazi, et al.
    MyJurnal
    Dengue Fever is a major public health issue in Malaysia. This study aimed to assess knowledge, attitude and practice regarding dengue fever and effectiveness of health education programme among 204 students of Alam Shah Science School, Cheras/Kuala Lumpur. Study design was a cross sectional study followed by an interventional (pre-post) Study. The self-administered questionnaire included questions on sociodemographic factors, knowledge, attitude and practice regarding dengue fever. Majority of respondents were Malays (96.6%), monthly family income more than RM 3000 (64.6%) and lives in urban area (64.6%). There was no significant association between knowledge status and socio-demographic factors (p-value>0.05). Most of the respondents had good knowledge (63.2%) good attitudes (79.9%) regarding dengue fever. However, practices to prevent dengue was poor (74.0%). Only about one-fifth of the respondents (18.2%) believed that dengue fever is a flu-like illness. Knowledge score was significantly increased after health education programme (p value
  14. Shakir Aizat Shahruddin, Pradheeksha Saseedaran, Aishah Salleh, Che Amira Ahmad Azmi, Nik Harriz Izwan Mohammad Alfaisal, Rusli Abdullah, et al.
    MyJurnal
    House officers who are suffering from stress, anxiety or depression are at risk of harm to themselves, colleagues and even patients, as well as affecting their livelihood. Objective: This
    study is aimed to find the prevalence of stress, anxiety and depression among house officers working
    in Kota Kinabalu, Sabah. Method: A cross-sectional study was done using a self-administered
    questionnaire containing socio-demographic factors and Depression, Anxiety and Stress Scale (DASS)
    21. Results: Prevalence of stress, anxiety and depression were 57.1%, 63.7%, and 42.9% respectively. Multiple logistic regression analysis found that house officers who thought of quitting Housemanship was associated with stress (adjusted OR = 3.64, 95% CI: (1.44, 9.20), p = 0.006), and depression (adjusted OR = 8.26, 95% CI: (2.96, 23.02), p < 0.001). House officers who perceives that they are bullied is associated with anxiety (adjusted OR = 4.16, 95% CI: (1.33, 13.07), p = 0.015). Work
    experience of the house officers in months have a protective effect to stress (adjusted OR = 0.92, 95%
    CI: (0.87, 0.97), p = 0.004), anxiety (adjusted OR = 0.93, 95% CI: (0.88, 0.98), p = 0.006) and
    depression (adjusted OR = 0.92, 95% CI: (0.87, 0.98), p = 0.006). Conclusion: The prevalence of
    stress, anxiety and depression among house officers in Kota Kinabalu, Sabah is high. Management
    should look into the findings and understand the importance of addressing this problem as it may
    affect patient care and safety, and the livelihood of house officers. Future studies identifying why
    house officers are thinking of quitting service and why they do feel that they are being bullied may be
    warranted.
  15. Shaiful Azuar Mohamad, Nurul Shahnadz Amir Hamzah, Mohd Yusof Hamzah, Rusli Ibrahim, Khairuddin Abd Rahim, Choong, Yew Keong, et al.
    MyJurnal
    The fermentation of Pleurotus pulmonarius (non-irradiated) and Pleurotus pulmonarius (irradiated) were carried out in Erlenmeyer flask 500 mL (working volume 250 mL) and screened for the presence of β-glucan (1, 3:1, 6). The biomass obtained was extracted using Modified Mizuno Method to get the endopolysaccharide. The endopolysaccharides of Pleurotus pulmonarius (irradiated) contained higher content of β-glucan (1, 3:1, 6) with 16.7 g/100g crude polysaccharide compared to the non-irradiated strain. The irradiated strain was chosen for further investigation. Fractionation of endopolysaccharide Pleurotus pulmonarius (irradiated) using column chromatograph yielded 7 fractions. The first fraction (F1) contained high molecular weight fraction ~ 105 Da which potential immunomodulation characteristics. The Pleurotus pulmonarius (irradiated) was produced in a 5 L air-lift bioreactor. The highest biomass was obtained at air flowrate of 2 L/min, yielding productivity of 2.56 g/L.d.
  16. Ainul Mardhiyah Mohd Razib, Goh TL, Nur Amanina Mazlan, Muhammad Fahmi Abdul Ghani, Tuan Rusli Tuan Mohamed, Abdul Ghani Rafek, et al.
    Sains Malaysiana, 2018;47:1413-1421.
    The stability of the limestone cliff at Gunung Kandu, Gopeng, Perak, Malaysia was assessed based on the Slope Mass
    Rating (SMR) system on 53 cross sections of the Gunung Kandu hill slopes. The slopes of Gunung Kandu were identified
    as class I (very good) to IV (poor). The kinematic analysis showed that 12 out of 53 hill slopes of Gunung Kandu were
    identified as having potential wedge, planar and toppling failures. The assessment showed that the stability of the western
    flanks can be classified as stable to unstable with the probability of failure from 0.2 to 0.6. The stability of the eastern and
    southern flanks range from very stable to partially stable with the probability of failure from 0.0 to 0.4. While the stability
    of northern flanks are from very stable to stable with the probability of failure of 0.0 - 0.2. This systematic approach
    offers a practical method especially for large area of rock slope stability assessment and the results from probability of
    failure values will help engineers to design adequate mitigation measures.
  17. Siti Nursyazwani Maadon, Sarini Ahmad Wakid, Iwana Izni Zainudin, Lili Syahani Rusli, Mohd Syahril Mohd Zan, Nor’Aishah Hasan, et al.
    Sains Malaysiana, 2018;47:3025-3030.
    Endophytic fungi are those living inside the host plant without causing any apparent negative effect on the host plant. Two
    isolates endophytic fungi from leaves and two isolates from root at Universiti Teknologi MARA (UiTM) Reserve Forest,
    Negeri Sembilan were successfully isolated and identified by morphology and molecular characteristic. Samples were
    surface sterilized and sub-cultured to obtain a pure culture. Characteristics of the isolates such as colony appearance,
    mycelial texture, conidia/spores and pigmentation were studied to explore their morphology. Isolates were also subjected to
    a PCR-based genotyping test. There were noticeable differences in morphological characteristics among the four isolates.
    Microscopic analysis showed four isolates consist of septa and conidia/spores. The pigmentation result showed that
    colony in A1leaf samples demonstrated an orange color on potato dextrose agar (PDA) media, colony in A1root demonstrate
    a black texture in PDA media while hairy colonies in the others two isolates showed a white color on PDA media. Based on
    molecular analyses the fungal genera showed 99-100% similarity with the related fungi recorded in the GenBank. Both
    morphology and molecular sequencing of internal transcribed spacer (ITS) regions of endophytic fungi showed that three
    isolates (A1root, C2leaf, and C3root) were grouped in Basidiomycota while one isolate (A1leaf) belonged to Ascomycota. The
    endophyte funguses were identified as Daldinia sp. (A1leaf), Polyporales sp. (A1root,) Lentinus sp. (C2leaf,) and Rigidoporus
    sp. (C3root). Overall, the new discoveries of isolated endophyte fungal have dyeing potential of fungal pigments which
    offer a viable alternative to natural vegetable and harmful synthetic dyes.
  18. Wong JM, Nur Amanina Mazlan, Ainul Mardhiyah Mohd Razib, Azimah Hussin, Abdul Ghani Rafek, Ailie Sofyiana Serasa, et al.
    Sains Malaysiana, 2018;47:59-66.
    The limestone hill of Batu Caves is slowly being turned into a recreation park for slope climbing, base jumping and cave exploring. Quantitative assessment on the stability of the cave is essential to ensure the safety of tourists and visitors. The aim of this study was to quantitatively assess the stability of Gua Damai, Batu Caves, Selangor, Malaysia by using the Q system for rock mass classification, together with other factors such as cave width and thickness of the cave roof. The stability of the limestone cave wall was evaluated using Slope Mass Rating (SMR). A discontinuity survey conducted along the slopes beneath the opening of the cave showed that the rock mass comprised of four major joint sets labeled as J1, J2, J3, and J4 with the dip directions and angles of 110˚/73˚, 325˚/87˚, 243˚/39˚ and 054˚/30˚, respectively. The result of kinematic analysis showed that the dip direction/dip angle of a potential wedge failure was 051˚/59˚. By referring to the ratio of cave roof thickness with cave width, the results showed that the cave is stable. Based on the relationship between Q system and the cave width, the stabilities of Section 4 of Gua Damai is stable while Section 1, 2, 3, 5, 6, 7 and 8 require supports. Based on SMR, the cave walls stability at Portion c, d, and f were not stable while Portion a, b, e and g were stable. Overall, the most stable part of the cave is Section 4 followed by Sections 5 and 2. Sections 1, 3 and 8 are moderately stable while Sections 6 and 7 have poor stability.
  19. Pan Y, Tiong KH, Abd-Rashid BA, Ismail Z, Ismail R, Mak JW, et al.
    Phytomedicine, 2014 Oct 15;21(12):1645-50.
    PMID: 25442272 DOI: 10.1016/j.phymed.2014.08.003
    This study was designed to investigate eight herbal active constituents (andrographolide, asiaticoside, asiatic acid, madecassic acid, eupatorin, sinensetin, caffeic acid, and rosmarinic acid) on their potential inhibitory effects on human cytochrome P450 1A2 (CYP1A2) activity. A fluorescence-based enzyme assay was performed by co-incubating human cDNA-expressed CYP1A2 with its selective probe substrate, 3-cyano-7-ethoxycoumarin (CEC), in the absence or presence of various concentrations of herbal active constituents. The metabolite (cyano-hydroxycoumarin) formed was subsequently measured in order to obtain IC50 values. The results indicated that only eupatorin and sinensetin moderately inhibited CYP1A2 with IC50 values of 50.8 and 40.2 μM, while the other active compounds did not significantly affect CYP1A2 activity with IC50 values more than 100 μM. Ki values further determined for eupatorin and sinensetin were 46.4 and 35.2 μM, respectively. Our data indicated that most of the investigated herbal constituents have negligible CYP1A2 inhibitory effect. In vivo studies however may be warranted to ascertain the inhibitory effect of eupatorin and sinensetin on CYP1A2 activity in clinical situations.
  20. Yoon YK, Ali MA, Wei AC, Choon TS, Ismail R
    Eur J Med Chem, 2015 Mar 26;93:614-24.
    PMID: 24996257 DOI: 10.1016/j.ejmech.2013.06.025
    A total of 51 novel benzimidazoles were synthesized by a 4-step reaction starting from basic compound 4-fluoro-3-nitrobenzoic acid under relatively mild reaction conditions. The structure of the novel benzimidazoles was confirmed by mass spectra as well as (1)H NMR spectroscopic data. Out of the 51 novel synthesized compounds, 42 of them were screened for their antimycobacterial activity against Mycobacterium tuberculosis H37Rv strain using BacTiter-Glo™ Microbial Cell Viability (BTG) method. Results of activity screened using Alamar Blue method was also provided for comparison purposes. Two of the novel benzimidazoles synthesized showed moderately good activity with IC50 of less than 15 μM. Compound 5g, ethyl 2-(4-(trifluoromethyl)phenyl)-1-(2-morpholinoethyl)-1H-benzo[d]imidazole-5-carboxylate, was found to be the most active with IC50 of 11.52 μM.
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