METHODOLOGY: The BEVQ has been authorized by the original author and was meticulously translated through a 10-step protocol. The content validity of the BEVQ was rigorously evaluated by four independent experts using the item-level content validity index (I-CVI), scale-level content validity index average (S-CVI/Ave), sum of the content validity index/universal agreement (S-CVI/UA), and the modified kappa statistic (κ m). The face validity was also conducted on 30 adolescents, ensuring clarity and comprehensive validation.
RESULTS: The translation process required minor modifications to ensure linguistic and cultural equivalence to the original questionnaire. The Arabic version of the BEVQ (BEVQ-A) achieved S-CVI/Ave scores ranging from 0.90 to 0.98 and S-CVI/UA scores ranging from 0.75 to 1.00. The modified kappa statistic (κ m) indicated that the majority of items were categorized as good to excellent. These scores confirmed that the BEVQ-A possessed robust content validity. Additionally, the BEVQ-A demonstratedcomprehensive and clear face validity, with a sum of face validity index (S-FVI) score of 0.97.
CONCLUSION: In conclusion, the Arabic-translated version of the BEVQ is a valid and reliable instrument for assessing total beverage consumption among Arabic-speaking adolescents.
METHODS: Different combinations of nitrogen sources, salts and pre-culture combinations were applied in the fermentation media and lovastatin yield was analysed chromatographically.
RESULT: The exclusion of MnSO4 ·5H2O, CuSO4·5H2O and FeCl3·6H2O were shown to significantly improve lovastatin production (282%), while KH2PO4, MgSO4·7H2O, and NaCl and ZnSO4·7H2O were indispensable for good lovastatin production. Simple nitrogen source (ammonia) was unfavourable for morphology, growth and lovastatin production. In contrast, yeast extract (complex nitrogen source) produced the highest lovastatin yield (25.52 mg/L), while powdered soybean favoured the production of co-metabolites ((+)-geodin and sulochrin). Intermediate lactose: yeast extract (5:4) ratio produced the optimal lovastatin yield (12.33 mg/L) during pre-culture, while high (5:2) or low (5:6) lactose to yeast extract ratio produced significantly lower lovastatin yield (7.98 mg/L and 9.12 mg/L, respectively). High spore concentration, up to 107 spores/L was shown to be beneficial for lovastatin, but not for co-metabolite production, while higher spore age was shown to be beneficial for all of its metabolites.
CONCLUSION: The findings from these investigations could be used for future cultivation of A. terreus in the production of desired metabolites.