MyMedR

Fusion genes in malignant neoplastic disorders of haematopoietic system

Saleem M, Yusoff NM

Hematology, 2016 Oct;21(9):501-12.

PMID: 26871368 DOI: 10.1080/10245332.2015.1106816

OBJECTIVES: The new World Health Organization's (WHO) classification of haematopoietic and lymphoid tissue neoplasms incorporating the recurrent fusion genes as the defining criteria for different haematopoietic malignant phenotypes is reviewed. The recurrent fusion genes incorporated in the new WHO's classification and other chromosomal rearrangements of haematopoietic and lymphoid tissue neoplasms are reviewed.

METHODOLOGY: Cytokines and transcription factors in haematopoiesis and leukaemic mechanisms are described. Genetic features and clinical implications due to the encoded chimeric neoproteins causing malignant haematopoietic disorders are reviewed.

RESULTS AND DISCUSSION: Multiple translocation partner genes are well known for leukaemia such as MYC, MLL, RARA, ALK, and RUNX1. With the advent of more sophisticated diagnostic tools and bioinformatics algorithms, an exponential growth in fusion genes discoveries is likely to increase.

CONCLUSION: Demonstration of fusion genes and their specific translocation breakpoints in malignant haematological disorders are crucial for understanding the molecular pathogenesis and clinical phenotype of cancer, determining prognostic indexes and therapeutic responses, and monitoring residual disease and relapse status.

Aerosol-based delivery of fibroblast cells for treatment of lung diseases

Kardia E, Yusoff NM, Zakaria Z, Yahaya B

J Aerosol Med Pulm Drug Deliv, 2014 Feb;27(1):30-4.

PMID: 23409833 DOI: 10.1089/jamp.2012.1020

Cell-based therapy has great potential to treat patients with lung diseases. The administration of cells into an injured lung is one method of repairing and replacing lost lung tissue. However, different types of delivery have been studied and compared, and none of the techniques resulted in engraftment of a high number of cells into the targeted organ. In this in vitro study, a novel method of cell delivery was introduced to investigate the possibility of delivering aerosolized skin-derived fibroblasts.

Fulltext Seropositive status of dengue virus infection among blood donors in North Malaysia

Harif NF, Kader ZS, Joshi SR, Yusoff NM

Asian J Transfus Sci, 2014 Jan;8(1):64.

PMID: 24678182 DOI: 10.4103/0973-6247.126702

Fulltext A Consanguinity Related Autosomal Translocation which Leads to Premature Ovarian Failure

Yik MY, Zain MM, Zakaria Z, Yusoff NM

J Clin Diagn Res, 2013 Feb;7(2):355-7.

PMID: 23543039 DOI: 10.7860/JCDR/2013/4354.2767

The premature ovarian failures with underlying chromosomal abnormalities are normally X-linked, although their associations with the autosomal and the Robertsonian translocations are also possible. Here, we are reporting a case of premature ovarian failure which was associated with a translocation between the long arm of chromosome 7 at q11.23 and the short arm of chromosome 5 at p15.3. The proband was a 26-year-old Malay woman who presented with premature ovarian failure, who was referred for cytogenetic testing due to the suspicion of a chromosomal anomaly. Her physical examination revealed that she had no abdominal or pelvic masses and that she had normal secondary sexual characteristics. Her medical history as well, revealed no points for concern. However, a consanguineous relationship existed, as the patient's paternal grandmother and maternal grandfather were biological cousins. Our present case indicated that region p15.3 of chromosome 5 and region q11.23 of chromosome 7 possibly carried essential genes for the ovarian function and that they postulated a link between the consanguinity and the chromosomal abnormalities.

Fulltext Preliminary comparative analysis of antibacterial effects of activated and non-activated of expired platelet concentrate by disc diffusion method

Zabidi MA, Yusoff NM, Kader ZS

Indian J Pathol Microbiol, 2012 Jan-Mar;55(1):47-51.

PMID: 22499300 DOI: 10.4103/0377-4929.94855

Platelets release more than 30 cytokines to provide primary hemostatic function. In addition, platelets are also known to release antimicrobial peptides upon activation by thrombin.

Association of ABO blood group B with myocardial infarction

Sheikh MK, Bahari MB, Yusoff NM, Knight A

J Coll Physicians Surg Pak, 2009 Aug;19(8):514-7.

PMID: 19651016 DOI: 08.2009/JCPSP.514517

The purpose of this study was to find out the association between blood group B and Myocardial Infarction (MI) in sample population in Malaysia.

Fulltext Association of ABO blood groups with diabetes mellitus

Kamil M, Al-Jamal HA, Yusoff NM

Libyan J Med, 2010 Feb 08;5.

PMID: 21483592 DOI: 10.3402/ljm.v5i0.4847

So far no studies have been performed in Malaysia to look at association of diabetes mellitus (DM) with blood groups. We studied the association of ABO blood groups with DM type 2.

Gene Silencing by RNAi in Mammalian Cells

Ponthan F, Yusoff NM, Soria NM, Heidenreich O, Coffey K

Curr Protoc Mol Biol, 2015 Jul 01;111:26.2.1-26.2.17.

PMID: 26131850 DOI: 10.1002/0471142727.mb2602s111

This unit provides information how to use short interfering RNA (siRNA) for sequence-specific gene silencing in mammalian cells. Several methods for siRNA generation and optimization, as well as recommendations for cell transfection and transduction, are presented.

Fulltext Blood transfusion services amidst the COVID-19 pandemic

Noordin SS, Yusoff NM, Karim FA, Chong SE

J Glob Health, 2021 Apr 17;11:03053.

PMID: 33884188 DOI: 10.7189/jogh.11.03053

An analysis of genetic association in opioid dependence susceptibility

Nagaya D, Zahari Z, Saleem M, Yahaya BH, Tan SC, Yusoff NM

J Clin Pharm Ther, 2018 Feb;43(1):80-86.

PMID: 28656735 DOI: 10.1111/jcpt.12585

WHAT IS KNOWN: Drug addiction is a novelty-seeking personality trait that is associated with the candidate genes OPRD1 (opioid delta receptors), OPRK1 (opioid kappa receptors) and PDYN (prodynorphin). However, associations between single nucleotide polymorphisms (SNPs) rs1042114 (80G>T) of the OPRD1 gene, rs702764 (843 A>G) of the OPRK1 gene, and rs910080 (3' UTR _743T>C), rs1997794 (5' UTR -381A>G) and rs1022563 (3' UTR) of the PDYN gene and novelty seeking remain controversial as reported results have not been reproducible.

OBJECTIVE: The goal of this study was to determine the frequencies of SNPs rs1042114, rs702764, rs1997794, rs1022563 and rs910080 in the Malaysian population and to study their association with opioid dependence in Malaysian Malays.

METHODS: A total of 459 Malay male with opioid dependence and 543 healthy male (controls) subjects were included in this study. SNPs were genotyped using the TaqMan SNP genotyping assay. Statistical analysis was performed using Golden Helix SVS software suite to identify the distribution of allele and genotype frequencies, and SNP-SNP interactions were also analysed in this study.

RESULTS AND DISCUSSION: SNP rs1042114 in the OPRD1 gene is strongly associated with opiate addiction (P=.0001). In individuals homozygous for this risk allele, the likelihood of opiate addiction is increased by a factor 1.62 (95% confidence interval (CI) 1.412-1.875). Polymorphic alleles at SNP rs702764 of OPRK1 were not associated with opioid dependence. A significant association between opioid dependence and SNP rs910080 of PDYN (P=.0217) was detected, but there was no association for SNPs rs199774 and rs1022563. A significant interaction was also identified between homozygous wild-type genotype TT of rs702764 with the risk genotypes TG/GG of rs1042114 (odds ratio (OR)=2.111 (95% CI 1.227-3.631), P=.0069) and with the risk genotypes GA/AA of rs910080 (OR=1.415 (95% CI 1.04-1.912), P=.0239).

WHAT IS NEW AND CONCLUSION: The results indicate that SNPs rs1042114 and rs910080 contribute to vulnerability to opioid dependence in the Malaysian Malay population. These results will help us to understand the effect of the SNPs and the SNP-SNP interaction on opioid dependence and may assist in efforts to screen vulnerable individuals and match them with individually tailored prevention and treatment strategies.

Direction of commissural axon projections in different regions of the spinal cord during chicken embryonic development

Li H, Yang C, Yusoff NM, Yahaya BH, Lin J

Neuroscience, 2017 09 01;358:269-276.

PMID: 28687312 DOI: 10.1016/j.neuroscience.2017.06.053

Few researchers have investigated the direction of commissural axon projections on the contralateral side of the vertebrate embryonic spinal cord, especially for comparison between its different regions. In this study, pCAGGS-GFP plasmid expression was limited to different regions of the chicken embryonic spinal cord (cervical, anterior limb, anterior thorax, posterior thorax and posterior limb) at E3 using in ovo electroporation with modified electrodes and optimal electroporation conditions. Then open-book technique was performed at E6 to analyze the direction of axon projections in different spinal cord regions. The results show that in the five investigated regions, most axons projected rostrally after crossing the floor plate while a minority projected caudally. And there was a significant difference between the rostral and caudal projection quantities (P<0.01). The ratio of rostral and caudal projections was significantly different between the five investigated regions (P<0.05), except between the cervical region and the anterior limb (P>0.05). The projections were most likely to be rostral for the posterior limb followed by the posterior thorax, cervical region, anterior limb and anterior thorax. Our data for the direction of the commissural axon projections will be helpful in the future analyses of axon projection mechanisms and spinal cord-brain circuit formation.

Gene frequencies of human platelet alloantigens in Kelantan, Malaysia

Bhaskar S, Abdullah JM, Yusoff NM, Prasad A

Singapore Med J, 2007 Jun;48(6):598-9.

PMID: 17538770

WITHDRAWN: Rh genotypes among Malaysian blood donors

Musa RH, Hassan A, Ayob Y, Yusoff NM

Transfus Apher Sci, 2014 Dec 12.

PMID: 26144905 DOI: 10.1016/j.transci.2014.12.001

This article has been withdrawn at the request of the author and editor. The Publisher apologizes for any inconvenience this may cause. The full Elsevier Policy on Article Withdrawal can be found at http://www.elsevier.com/locate/withdrawalpolicy.

Fulltext Hereditary spherocytosis in a malay patient with chronic haemolysis

Sheikh MK, Yusoff NM, Kaur G, Khan FA

Malays J Med Sci, 2007 Jul;14(2):54-7.

PMID: 22993492 MyJurnal

This case report describes a 35-year-old lady who presented with generalized weakness and lethargy of two weeks duration and jaundice of more than 20 years duration. Her initial workup was suggestive of haemolysis and blood film showed a leucoerythoblastic picture with moderate microspherocytes. She was finally diagnosed as a case of hereditary spherocytosis after ruling out other possible causes of chronic haemolysis and supported by an abnormal osmotic fragility test, although family members refused for screening. Hereditory spherocytosis is uncommon in Malay population and presentation with jaundice of 20 years duration with leucoerythroblastic picture on blood film were interesting features in this case. Patient is being followed closely for need of splenectomy in near future as per severity of haemolysis and currently being managed with folic acid supplement.

Fulltext A Cell Internalizing Antibody Targeting Capsid Protein (p24) Inhibits the Replication of HIV-1 in T Cells Lines and PBMCs: A Proof of Concept Study

Ali SA, Teow SY, Omar TC, Khoo AS, Choon TS, Yusoff NM

PLoS One, 2016;11(1):e0145986.

PMID: 26741963 DOI: 10.1371/journal.pone.0145986

There remains a need for newer therapeutic approaches to combat HIV/AIDS. Viral capsid protein p24 plays important roles in HIV pathogenesis. Peptides and small molecule inhibitors targeting p24 have shown to inhibit virus replication in treated cell. High specificity and biological stability of monoclonal antibodies (mAbs) make them an attractive contender for in vivo treatments. However, mAbs do not enter into cells, thus are restricted to target surface molecules. This also makes targeting intracellular HIV-1 p24 a challenge. A mAb specific to p24 that can internalize into the HIV-infected cells is hypothesized to inhibit the virus replication. We selected a mAb that has previously shown to inhibit p24 polymerization in an in vitro assay and chemically conjugated it with cell penetrating peptides (CPP) to generate cell internalizing anti-p24 mAbs. Out of 8 CPPs tested, κFGF-MTS -conjugated mAbs internalized T cells most efficiently. At nontoxic concentration, the κFGF-MTS-anti-p24-mAbs reduced the HIV-1 replication up to 73 and 49% in T-lymphocyte and PBMCs respectively. Marked inhibition of HIV-1 replication in relevant cells by κFGF-MTS-anti-p24-mAbs represents a viable strategy to target HIV proteins present inside the cells.

Fulltext Molecular basis of Rh blood group system in the Malaysian population

Musa RH, Muhamad NA, Hassan A, Ayob Y, Yusoff NM

Asian J Transfus Sci, 2015 Jan-Jun;9(1):48-54.

PMID: 25722573 DOI: 10.4103/0973-6247.150951

Rh molecular studies have been previously mainly conducted in Caucasians and African population. There is a limited data on the molecular basis for Rh genotypes among Asians.

Fulltext The effects of virgin coconut oil (VCO) as supplementation on quality of life (QOL) among breast cancer patients

Law KS, Azman N, Omar EA, Musa MY, Yusoff NM, Sulaiman SA, et al.

Lipids Health Dis, 2014;13:139.

PMID: 25163649 DOI: 10.1186/1476-511X-13-139

Breast cancer is the most common cancer amongst Malaysian women. Both the disease and its treatment can disrupt the lives of the woman and adversely affect all aspects of life and thus can alter a woman's quality of life. The aim of this study was to examine the effect of virgin coconut oil (VCO) on the quality of life (QOL) of patients diagnosed with breast cancer.

Fulltext Identification of novel mutations in exon 14 of the f8 gene in malaysian patients with severe hemophilia a

Moses EJ, Ling SP, Al-Hassan FM, Karim FA, Yusoff NM

Indian J Clin Biochem, 2012 Apr;27(2):207-8.

PMID: 23543988 DOI: 10.1007/s12291-011-0161-z

Fulltext Quality indicators for discarding blood in the National Blood Center, Kuala Lumpur

Morish M, Ayob Y, Naim N, Salman H, Muhamad NA, Yusoff NM

Asian J Transfus Sci, 2012 Jan;6(1):19-23.

PMID: 22623837 DOI: 10.4103/0973-6247.95045

The implementation of quality system and continuous evaluation of all activities of the Blood Transfusion Services (BTS) can help to achieve the maximum quantity and quality of safe blood. Optimizing blood collection and processing would reduce the rate of discard and improve the efficiency of the BTS. The objective of this study is to determine the rate of discard of blood and blood component and identify its reasons at the National Blood Centre (NBC), Kuala Lumpur, during the year of 2007 in order to introduce appropriate intervention.

Fulltext Chromosomal 16p microdeletion in Rubinstein-Taybi syndrome detected by oligonucleotide-based array comparative genomic hybridization: a case report

Mohd Fadley MA, Ismail A, Thong MK, Yusoff NM, Zakaria Z

J Med Case Rep, 2012;6:30.

PMID: 22269667 DOI: 10.1186/1752-1947-6-30

Chromosomal aberrations of chromosome 16 are uncommon and submicroscopic deletions have rarely been reported. At present, a cytogenetic or molecular abnormality can only be detected in 55% of Rubinstein-Taybi syndrome patients, leaving the diagnosis in 45% of patients to rest on clinical features only. Interestingly, this microdeletion of 16 p13.3 was found in a young child with an unexplained syndromic condition due to an indistinct etiological diagnosis. To the best of our knowledge, no evidence of a microdeletion of 16 p13.3 with contiguous gene deletion, comprising cyclic adenosine monophosphate-response element-binding protein and tumor necrosis factor receptor-associated protein 1 genes, has been described in typical Rubinstein-Taybi syndrome.

Displaying publications 1 - 20 of 57 in total