Browse publications by year: 2010

  1. Dayangku Norsuhazenah PS, Baki MM, Mohamad Yunus MR, Sabir Husin Athar PP, Abdullah S
    Ann Acad Med Singap, 2010 Jul;39(7):565-4.
    PMID: 20697675
    INTRODUCTION: In laryngectomised patients, tracheoesophageal speech is the gold standard for voice rehabilitation. This study evaluated complications related to the tracheoesophageal puncture (TEP) and the success rate in voice prosthesis after total laryngectomy at our institution over a 10-year period.

    MATERIALS AND METHODS: A retrospective review of 22 TEPs was performed between January 1998 and December 2008. The timing of TEP, type of voice prosthesis, surgical and prosthesis-related complications, and TEP closure were noted.

    RESULTS: Eighteen percent of the patients underwent primary and 82% secondary TEP. Our patients were predominantly males (95.4%) of Chinese descent with a mean age of 62.1 years. The types of voice prostheses used were ProvoxTM (n = 15), Voicemasters (n = 6), and Blom-Singer (n = 1). Prosthesis- related complications occurred in 77.3%. Notable complications were leakage (82.5%), prosthesis displacement (41.2%), intractable aspiration (29.4%), and aspiration of prosthesis (23.5%). The most common surgical-related complication was tracheostomal stenosis. An array of interventions comprising resizing or changing prosthesis type, nasogastric catheter insertion, stomaplasty, purse string suturing, and bronchoscopic removal of bronchial aspirated prosthesis were implemented to address encountered complications. In a mean follow-up of 34.8 months, 68.2% of patients achieved functional tracheoesophageal speech (75% of primary TEP and 67% of secondary TEP). There were 7 TEP closures indicated by persistent leakage, recurrent dislodgement, phonatory failure and, in 1 patient, persistent pain.

    CONCLUSIONS: TEP has become an integral part in the rehabilitation of a laryngectomee. However, management of the frequent complications related to TEP requires specific efforts and specialistic commitments in order to treat them.

    MeSH terms: Academic Medical Centers/statistics & numerical data; Adult; Aged; Constriction, Pathologic/etiology; Constriction, Pathologic/epidemiology; Female; Humans; Laryngectomy/rehabilitation*; Larynx, Artificial/adverse effects*; Malaysia/epidemiology; Male; Middle Aged; Punctures/adverse effects*; Retrospective Studies; Speech, Alaryngeal*; Trachea/surgery; Incidence; Equipment Failure Analysis*
  2. Kessler M, Jonas R, Cicuzza D, Kluge J, Piatek K, Naks P, et al.
    Plant Biol (Stuttg), 2010 Sep 1;12(5):788-93.
    PMID: 20701702 DOI: 10.1111/j.1438-8677.2009.00270.x
    The colonisation of land by plants may not have been possible without mycorrhizae, which supply the majority of land plants with nutrients, water and other benefits. In this sense, the mycorrhization of basal groups of land plants such as ferns and lycophytes is of particular interest, yet only about 9% of fern and lycophyte species have been sampled for their mycorrhization status, and no community-level analyses exist for tropical fern communities. In the present study, we screened 170 specimens of ferns and lycophytes from Malaysia and Sulawesi (Indonesia), representing 126 species, and report the mycorrhization status for 109 species and 19 genera for the first time. Mycorrhizal colonisations were detected in 96 (56.5%) of the specimens, 85 of which corresponded to arbuscular mycorrhizae (AMF), three to dark-septate endophytes (DSE) and four to mixed colonisations (AMF + DSE). DSE colonisations were lower than in comparable samples of ferns from the Andes, suggesting a geographical or taxonomic pattern in this type of colonisation. Epiphytes had significantly lower levels of colonisation (26.1%) than terrestrial plants (70.7%), probably due to the difficulty of establishment of mycorrhizal fungi in the canopy habitat.
    MeSH terms: Indonesia; Malaysia; Ecosystem*; Plant Roots/microbiology; Ferns/microbiology*; Mycorrhizae/classification*
  3. Ma NH, Teh CL, Rapaee A, Lau KB, Fong AY, Hi S, et al.
    Int J Rheum Dis, 2010 Aug;13(3):223-9.
    PMID: 20704618 DOI: 10.1111/j.1756-185X.2010.01533.x
    INTRODUCTION: Rheumatoid arthritis (RA) patients who have active disease with longer disease duration have been reported to have increased risk of cardiovascular events compared to the normal population.
    OBJECTIVE: The primary aim of our study is to ascertain the prevalence of significant asymptomatic coronary artery disease (CAD) in Asian RA patients who are in remission using multi-detector computed tomography (MDCT). The secondary aims of our study are the usage of pulse wave velocity and the biomarkers N-terminal pro-brain natriuretic peptide (NT-proBNP) and high-senstivity C-reactive protein (hs-CRP) to detect subclinical atherosclerosis in RA patients.
    METHODS: We performed a comparative cross-sectional study of 47 RA patients who were in remission with a control group of non-RA patients with a history of atypical chest pain in Sarawak General Hospital from November 2008 to February 2009. All patients underwent 64-slice MDCT, assessment of arterial stiffness using the SphygmoCor test and blood analysis for NT-proBNP and hsCRP.
    RESULTS: There were 94 patients in our study with a mean age of 50 +/- 8.8 years. The RA and control patients in each group were matched in terms of traditional CV risk factors. Our RA patients had a median disease duration of 3 years (IQR 5.5). MDCT showed evidence of CAD in nine (19.1%) RA patients and three (6.4%) control patients (P = 0.06). There was no significant association between pulse wave velocity (PWV) and presence of CAD in our RA group. There was no significant correlation between PWV with levels of proBNP or hsCRP in our RA patients.
    CONCLUSIONS: In our current pilot study with the limitation of small sample size, RA was not associated with an increased risk of CAD in our RA patients who were in remission. Larger studies of CAD in Asian RA patients are needed to confirm our current finding.
    Study site: Sarawak General Hospital, Kuching, Sarawak, Malaysia
    MeSH terms: Adult; Arteries/physiopathology; Arthritis, Rheumatoid/ethnology*; Arthritis, Rheumatoid/therapy; C-Reactive Protein/analysis; Coronary Artery Disease/blood; Coronary Artery Disease/ethnology*; Coronary Artery Disease/radiography; Coronary Artery Disease/ultrasonography; Cross-Sectional Studies; Elasticity; Female; Humans; Malaysia; Malaysia/epidemiology; Male; Middle Aged; Peptide Fragments/blood; Pilot Projects; Remission Induction; Risk Factors; Tomography, X-Ray Computed; Biomarkers/blood; Prevalence; Chi-Square Distribution; Case-Control Studies; Coronary Angiography/methods; Risk Assessment; Natriuretic Peptide, Brain/blood; Asian Continental Ancestry Group/statistics & numerical data*; Asymptomatic Diseases
  4. Zahed MA, Aziz HA, Isa MH, Mohajeri L, Mohajeri S
    Bioresour Technol, 2010 Dec;101(24):9455-60.
    PMID: 20705460 DOI: 10.1016/j.biortech.2010.07.077
    To determine the influence of nutrients on the rate of biodegradation, a five-level, three-factor central composite design (CCD) was employed for bioremediation of seawater artificially contaminated with crude oil. Removal of total petroleum hydrocarbons (TPH) was the dependent variable. Samples were extracted and analyzed according to US-EPA protocols. A significant (R(2)=0.9645, P<0.0001) quadratic polynomial mathematical model was generated. Removal from samples not subjected to optimization and removal by natural attenuation were 53.3% and 22.6%, respectively. Numerical optimization was carried out based on desirability functions for maximum TPH removal. For an initial crude oil concentration of 1g/L supplemented with 190.21 mg/L nitrogen and 12.71 mg/L phosphorus, the Design-Expert software predicted 60.9% hydrocarbon removal; 58.6% removal was observed in a 28-day experiment.
    MeSH terms: Analysis of Variance; Biodegradation, Environmental; Models, Chemical; Nitrogen/analysis; Petroleum/analysis*; Phosphorus/analysis; Seawater/analysis*; Time Factors; Environmental Restoration and Remediation/methods*
  5. Sreekantan S, Saharudin KA, Lockman Z, Tzu TW
    Nanotechnology, 2010 Sep 10;21(36):365603.
    PMID: 20705970 DOI: 10.1088/0957-4484/21/36/365603
    In this work, 18.5 microm titanium oxide (TiO(2)) nanotube arrays were formed by the anodization of titanium (Ti) foil in ethylene glycol containing 1 wt% water and 5 wt% fluoride for 60 min at 60 V. The fast growth rate of the nanotube arrays at 308 nm min(-1) was achieved due to the excess fluoride content and the limited amount of water in ethylene glycol used for anodization. Limited water content and excess fluoride in ethylene glycol inhibited the formation of a thick barrier layer by increasing the dissolution rate at the bottom of the nanotubes. This eased the transport of titanium, fluorine and oxygen ions, and allowed the nanotubes to grow deep into the titanium foil. At the same time, the neutral condition offered a protective environment along the tube wall and pore mouth, which minimized lateral and top dissolution. Results from x-ray photoelectron spectra revealed that the TiO(2) nanotubes prepared in ethylene glycol contained Ti, oxygen (O) and carbon (C) after annealing. The photocatalytic activity of the nanotube arrays produced was evaluated by monitoring the degradation of methyl orange. Results indicate that a nanotube with an average diameter of 140 nm and an optimal tube length of 18.5 microm with a thin tube wall (20 nm) is the optimum structure required to achieve high photocatalytic reaction. In addition, the existence of carbon, high degree of anatase crystallinity, smooth wall and absence of fluorine enhanced the photocatalytic activity of the sample.
  6. Soosean C, Marimuthu K, Sudhakaran S, Xavier R
    Eur Rev Med Pharmacol Sci, 2010 Jul;14(7):605-11.
    PMID: 20707250
    The efficacy of dietary inclusion of various parts of mangosteen (Garcinia mangostana L.) extract on growth and hematological parameters of African catfish (Clarias gariepinus) fingerlings were investigated.
    MeSH terms: Animals; Catfishes/growth & development*; Erythrocyte Count; Hemoglobins/metabolism; Leukocyte Count; Plant Extracts/pharmacology*; Aquaculture; Plant Leaves; Plant Shoots; Garcinia mangostana/chemistry*
  7. Wei OY, Xavier R, Marimuthu K
    Eur Rev Med Pharmacol Sci, 2010 Aug;14(8):675-81.
    PMID: 20707287
    The objective of this study is to gain a better understanding of the antimicrobial properties of the mucus extract of snakehead fish, Channa striatus against selected human and fish pathogenic microbes.
    MeSH terms: Animals; Anti-Bacterial Agents/isolation & purification; Anti-Bacterial Agents/pharmacology*; Bacteria/drug effects*; Bacteria/pathogenicity; Fishes/metabolism*; Microbial Sensitivity Tests; Mucus/chemistry*; Aeromonas hydrophila/drug effects
  8. Zainuddin A, Chua KH, Abdul Rahim N, Makpol S
    BMC Mol. Biol., 2010;11:59.
    PMID: 20707929 DOI: 10.1186/1471-2199-11-59
    Several genes have been used as housekeeping genes and choosing an appropriate reference gene is important for accurate quantitative RNA expression in real time RT-PCR technique. The expression levels of reference genes should remain constant between the cells of different tissues and under different experimental conditions. The purpose of this study was to determine the effect of different experimental treatments on the expression of glyceraldehyde 3-phosphate dehydrogenase (GAPDH) mRNA so that the reliability of GAPDH as reference gene for quantitative real time RT-PCR in human diploid fibroblasts (HDFs) can be validated. HDFs in 4 different treatment groups viz; young (passage 4), senescent (passage 30), H2O2-induced oxidative stress and gamma-tocotrienol (GTT)-treated groups were harvested for total RNA extraction. Total RNA concentration and purity were determined prior to GAPDH mRNA quantification. Standard curve of GAPDH expression in serial diluted total RNA, melting curve analysis and agarose gel electrophoresis were used to determine the reliability of GAPDH as reference gene.
    MeSH terms: beta-Galactosidase/genetics; beta-Galactosidase/metabolism; Cells, Cultured; Child; Chromans/pharmacology; Diploidy*; Fibroblasts/cytology; Fibroblasts/drug effects; Fibroblasts/physiology*; Glyceraldehyde-3-Phosphate Dehydrogenases/genetics*; Glyceraldehyde-3-Phosphate Dehydrogenases/metabolism; Humans; Hydrogen Peroxide/pharmacology; Male; Reference Standards; RNA, Messenger/genetics; RNA, Messenger/metabolism*; Vitamin E/analogs & derivatives; Vitamin E/pharmacology; Oxidants/pharmacology; Cell Aging/genetics; Oxidative Stress/drug effects; Cell Shape
  9. Ong MP, Sam IC, Azwa H, Mohd Zakaria IE, Kamarulzaman A, Wong MH, et al.
    J Infect, 2010 Nov;61(5):440-2.
    PMID: 20708031 DOI: 10.1016/j.jinf.2010.08.001
    MeSH terms: Adolescent; Adult; Aged; Anti-Bacterial Agents/economics; Anti-Bacterial Agents/therapeutic use; Child; Child, Preschool; Female; Hospitalization/economics*; Hospitals, Teaching; Humans; Infant; Infant, Newborn; Influenza, Human/diagnosis; Influenza, Human/drug therapy; Influenza, Human/economics*; Malaysia; Male; Middle Aged; Polymerase Chain Reaction/economics; Young Adult; Pandemics
  10. Zakaria NA, Azamathulla HM, Chang CK, Ghani AA
    Sci Total Environ, 2010 Oct 1;408(21):5078-85.
    PMID: 20708217 DOI: 10.1016/j.scitotenv.2010.07.048
    This paper presents Gene-Expression Programming (GEP), which is an extension to the genetic programming (GP) approach to predict the total bed material load for three Malaysian rivers. The GEP is employed without any restriction to an extensive database compiled from measurements in the Muda, Langat, and Kurau rivers. The GEP approach demonstrated a superior performance compared to other traditional sediment load methods. The coefficient of determination, R(2) (=0.97) and the mean square error, MSE (=0.057) of the GEP method are higher than those of the traditional method. The performance of the GEP method demonstrates its predictive capability and the possibility of the generalization of the model to nonlinear problems for river engineering applications.
    MeSH terms: Computer Simulation*; Environmental Monitoring/methods*; Malaysia; Water Movements; Water Pollutants/chemistry*; Geologic Sediments/chemistry*; Rivers/chemistry*; Geological Processes
  11. Kassim M, Achoui M, Mansor M, Yusoff KM
    Fitoterapia, 2010 Dec;81(8):1196-201.
    PMID: 20708657 DOI: 10.1016/j.fitote.2010.07.024
    We investigated the effects of honey and its methanol and ethyl acetate extracts on inflammation in animal models. Rats' paws were induced with carrageenan in the non-immune inflammatory and nociceptive model, and lipopolysaccharide (LPS) in the immune inflammatory model. Honey and its extracts were able to inhibit edema and pain in inflammatory tissues as well as showing potent inhibitory activities against NO and PGE(2) in both models. The decrease in edema and pain correlates with the inhibition of NO and PGE(2). Phenolic compounds have been implicated in the inhibitory activities. Honey is potentially useful in the treatment of inflammatory conditions.
    MeSH terms: Animals; Honey/analysis*; Inflammation/drug therapy*; Inflammation/metabolism; Malaysia; Male; Nitric Oxide/antagonists & inhibitors*; Dinoprostone/antagonists & inhibitors*; Rats, Sprague-Dawley; Rats
  12. Zyoud SH, Awang R, Sulaiman SA, Al-Jabi SW
    Pharmacoepidemiol Drug Saf, 2010 Oct;19(10):1064-70.
    PMID: 20712021 DOI: 10.1002/pds.1955
    To investigate the relationship between different types of adverse drug reaction (ADR) and late time to N-acetylcysteine (NAC) infusion in patients presenting to the hospital with acetaminophen overdose.
    MeSH terms: Acetaminophen/poisoning*; Acetaminophen/therapeutic use; Acetylcysteine/administration & dosage; Acetylcysteine/adverse effects; Acetylcysteine/therapeutic use*; Adult; Anaphylaxis/chemically induced; Anaphylaxis/drug therapy; Antidotes/administration & dosage; Antidotes/adverse effects; Antidotes/therapeutic use*; Female; Humans; Male; Retrospective Studies; Risk Factors; Time Factors; Young Adult; Drug Overdose/drug therapy*
  13. Tee HP, Corte C, Al-Ghamdi H, Prakoso E, Darke J, Chettiar R, et al.
    World J Gastroenterol, 2010 Aug 21;16(31):3905-10.
    PMID: 20712051
    AIM: To study the significance of cap-fitted colonoscopy in improving cecal intubation time and polyp detection rate.

    METHODS: This study was a prospective randomized controlled trial conducted from March 2008 to February 2009 in a tertiary referral hospital at Sydney. The primary end point was cecal intubation time and the secondary endpoint was polyp detection rate. Consecutive cases of total colonoscopy over a 1-year period were recruited. Randomization into either standard colonoscopy (SC) or cap-assisted colonoscopy (CAC) was performed after consent was obtained. For cases randomized to CAC, one of the three sizes of cap was used: D-201-15004 (with a diameter of 15.3 mm), D-201-14304 (14.6 mm) and D-201-12704 (13.0 mm). All of these caps were produced by Olympus Medical Systems, Japan. Independent predictors for faster cecal time and better polyp detection rate were also determined from this study.

    RESULTS: There were 200 cases in each group. There was no significant difference in terms of demographic characteristics between the two groups. CAC, when compared to the SC group, had no significant difference in terms of cecal intubation rate (96.0% vs 97.0%, P = 0.40) and time (9.94 +/- 7.05 min vs 10.34 +/- 6.82 min, P = 0.21), or polyp detection rate (32.8% vs 31.3%, P = 0.75). On the subgroup analysis, there was no significant difference in terms of cecal intubation time by trainees (88.1% vs 84.8%, P = 0.40), ileal intubation rate (82.5% vs 79.0%, P = 0.38) or total colonoscopy time (23.24 +/- 13.95 min vs 22.56 +/- 9.94 min, P = 0.88). On multivariate analysis, the independent determinants of faster cecal time were consultant-performed procedures (P < 0.001), male patients (P < 0.001), non-usage of hyoscine (P < 0.001) and better bowel preparation (P = 0.01). The determinants of better polyp detection rate were older age (P < 0.001), no history of previous abdominal surgery (P = 0.04), patients not having esophagogastroduodenoscopy in the same setting (P = 0.003), trainee-performed procedures (P = 0.01), usage of hyoscine (P = 0.01) and procedures performed for polyp follow-up (P = 0.01). The limitations of the study were that it was a single-center experience, no blinding was possible, and there were a large number of endoscopists.

    CONCLUSION: CAC did not significantly different from SC in term of cecal intubation time and polyp detection rate.

    MeSH terms: Adult; Aged; Cathartics/therapeutic use; Clinical Competence; Colonic Polyps/diagnosis*; Colonoscopy/adverse effects; Colonoscopy/methods*; Equipment Design; Female; Humans; Male; Middle Aged; New South Wales; Predictive Value of Tests; Prospective Studies; Scopolamine Hydrobromide/therapeutic use; Sex Factors; Time Factors; Chi-Square Distribution; Linear Models; Muscarinic Antagonists/therapeutic use; Colonoscopes*
  14. Momtaz YA, Ibrahim R, Hamid TA, Yahaya N
    Omega (Westport), 2010;61(2):145-62.
    PMID: 20712141
    Spousal death is one the most stressful life events that seriously affects the psychological well being of widowed. This study examined the mediating effects of social and personal religiosity on the psychological well being of widowed elderly people. The sample for this study was comprised of 1367 widowed and married elderly Muslims from Malaysia. Psychological well being, religiosity, and physical health were measured using WHO-5 Well being Index, Intrinsic Extrinsic religiosity scale, and a checklist of 16 physical health problems, respectively. Data analysis was conducted using the Statistical Package for Social Sciences (version-13). As expected, bivariate correlation analysis revealed that widowhood is statistically and negatively associated with psychological well being. Results of multiple hierarchical regression analyses and Sobel test showed that only the indirect effect of widowhood through personal religiosity was statistically significant (Sobel = -2.79, p < .01). Sobel test for social religiosity was not significant (Sobel = -1.54, p > .05). The results of this study confirmed earlier studies, which found that widowhood negatively affects psychological well being of elderly people. Overall, the findings show that the potential solace provided by religiosity can decrease the negative effects of widowhood on the psychological well being of widowed elderly people.
    MeSH terms: Adaptation, Psychological; Aged; Aged, 80 and over; Attitude to Death/ethnology*; Bereavement*; Female; Humans; Islam/psychology*; Life Change Events; Loneliness; Malaysia; Male; Surveys and Questionnaires; Regression Analysis; Religion and Psychology*; Social Perception; Social Support; Widowhood/ethnology; Widowhood/psychology*
  15. Beng TS, Chin LE
    J Palliat Med, 2010 Aug;13(8):1037-8.
    PMID: 20712471 DOI: 10.1089/jpm.2009.0314
    We present a case of gross lower limb edema in a 21-year-old man with an intra-abdominal malignant fibrous histiocytoma. He had a 1-month history of lower limb edema secondary to inferior vena caval obstruction. His edema failed to respond to a combination of diuretics, oral frusemide 40 mg daily and oral spironolactone 100 mg daily. Subcutaneous drainage of both lower limbs with butterfly needles was performed with minimal improvement. However, he gained significant symptomatic relief with multiple subcutaneous punctures and stoma bag drainage. A total of 12.8 L of serous fluid was drained with this simple and effective method. This is the first report of the use of this method.
    MeSH terms: Adult; Clinical Protocols; Constriction, Pathologic; Drainage/instrumentation; Drainage/methods*; Edema/etiology; Edema/therapy*; Humans; Leg*; Male; Palliative Care/methods*; Punctures/instrumentation; Punctures/methods*; Retroperitoneal Neoplasms/complications; Retroperitoneal Neoplasms/therapy; Vena Cava, Inferior; Fatal Outcome; Patient Selection; Histiocytoma, Malignant Fibrous/complications; Histiocytoma, Malignant Fibrous/therapy
  16. Chen J, Teo YY, Toh DS, Sung C
    Pharmacogenomics, 2010 Aug;11(8):1077-94.
    PMID: 20712526 DOI: 10.2217/pgs.10.79
    The frequencies of alleles implicated in drug-response variability provide vital information for public health management. Differences in frequencies between genetically diverse groups of individuals can hamper drug assessments, particularly in populations where clinical data are not readily available.
    MeSH terms: China/ethnology; Drug and Narcotic Control*; Gene Frequency*; Genetics, Population; Humans; India/ethnology; Malaysia/ethnology; Pharmacogenetics/legislation & jurisprudence; Pharmacogenetics/methods*; Singapore/epidemiology; Polymorphism, Single Nucleotide*; Databases, Genetic*; European Continental Ancestry Group/genetics*; Asian Continental Ancestry Group/genetics*
  17. Naidu R, Har YC, Taib NA
    Pathol. Int., 2010 Sep;60(9):614-20.
    PMID: 20712647 DOI: 10.1111/j.1440-1827.2010.02568.x
    The aim of the present study was to evaluate the association between the Glyoxalase I (GLOI) Ala111Glu polymorphism and breast cancer risk among the major Malaysian ethnic groups, the Malays, Chinese and Indians, as well as clinico-pathological characteristics of these patients. Genotyping of GLOI gene was performed on blood samples obtained from 387 patients and 252 normal healthy women who had no history of any malignancy using the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. The genotype and allele frequencies of GLOI polymorphism were not significantly different between the patients and normal individuals among the Malays (P= 0.721, 0.402), Chinese (P= 0.208, 0.079) and Indians (P= 0.612, 0.349), respectively. The Malay, Chinese and Indian women who were Glu/Glu homozygotes (P= 0.419, 0.093, 0.367), Ala/Glu heterozygotes (P= 0.648, 0.182, 0.402) and carriers of Glu allele (P= 0.402, 0.079, 0.349), respectively, were not associated with breast cancer risk. The Glu allele genotype was significantly associated with absence of progesterone receptor (P= 0.036). Thus, the polymorphic variant of the GLOI gene might not be a useful genetic marker to identify Malaysian Malay, Chinese or Indian women who could be at greater risk of developing breast cancer.

    Study site: Universiti Malaya Medical Centre (UMMC)
    MeSH terms: Adult; Aged; Aged, 80 and over; Breast Neoplasms/genetics*; China/ethnology; Female; Gene Frequency/genetics; Genotype; Hospitals, University; Humans; India/ethnology; Lactoylglutathione Lyase/genetics*; Malaysia; Middle Aged; Polymorphism, Genetic/genetics*; Receptors, Progesterone/genetics*; Risk Factors; Case-Control Studies; Genetic Predisposition to Disease/genetics
  18. Moharam BA, Jantan I, Ahmad Fb, Jalil J
    Molecules, 2010 Aug;15(8):5124-38.
    PMID: 20714290 DOI: 10.3390/molecules15085124
    Nine essential oils, hydrodistilled from different parts of five Goniothalamus species (G. velutinus Airy-Shaw, G. woodii Merr., G. clemensii Ban, G. tapis Miq. and G. tapisoides Mat Salleh) were evaluated for their ability to inhibit platelet aggregation in human whole blood using an electrical impedance method and their inhibitory effects on platelet activating factor (PAF) receptor binding with rabbit platelets using 3H-PAF as a ligand. The chemical composition of the oils was analyzed by gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS). The bark oil of G. velutinus was the most effective sample as it inhibited both arachidonic acid (AA) and ADP-induced platelet aggregation with IC(50) values of 93.6 and 87.7 microg/mL, respectively. Among the studied oils, the bark oils of G. clemensii, G. woodii, G. velutinus and the root oil of G. tapis showed significant inhibitory effects on PAF receptor binding, with IC(50 )values ranging from 3.5 to 10.5 microg/mL. The strong PAF antagonistic activity of the active oils is related to their high contents of sesquiterpenes and sesquiterpenoids, and the individual components in the oils could possibly produce a synergistic effect in the overall antiplatelet activity of the oils.
    MeSH terms: Animals; Aspirin/pharmacology; Humans; Oils, Volatile/analysis; Oils, Volatile/pharmacology*; Platelet Aggregation/drug effects*; Platelet Aggregation Inhibitors/pharmacology*; Platelet Membrane Glycoproteins/antagonists & inhibitors*; Protein Binding/drug effects; Rabbits; Reference Standards; Terpenes/pharmacology; Plant Leaves/chemistry; Plant Roots/chemistry; Inhibitory Concentration 50; Plant Bark/chemistry; Receptors, G-Protein-Coupled/antagonists & inhibitors*; Goniothalamus/chemistry*
  19. Tan YO, Han S, Lu YS, Yip CH, Sunpaweravong P, Jeong J, et al.
    Cancer, 2010 Dec 1;116(23):5348-57.
    PMID: 20715159 DOI: 10.1002/cncr.25476
    Overexpression of the epidermal growth factor receptor-related gene ErbB2 occurs in 18% to 25% of patients with breast cancer in Western countries and is associated with a poor prognosis. The prevalence of ErbB2-positive tumors in Asia is unclear, partly because data are limited. The objective of this review was to summarize the reported prevalence of ErbB2-positive tumors from a large sample of Asian patients and to examine ErbB2 assessment methods in Asia. From searches of MEDLINE, local language journals, and local and international conference proceedings as well as locoregional breast cancer experts' recommendations, the authors selected up to 5 studies each from India, Korea, Malaysia, the Philippines, Singapore, Taiwan, and Thailand that reported ErbB2 results based on assessment with immunohistochemistry (IHC) and/or fluorescence in situ hybridization (FISH). The reported prevalence of ErbB2-positive tumors in 22 studies on 24,671 patients, of whom 14,398 patients were assessed for ErbB2 status, varied widely (range, 6%-65%) as did the assessment methods used. Most studies (n=21) used IHC to assess ErbB2 status, but definitions for positivity varied. When robust assessment methods were used, the median prevalence was 19% based on strong IHC staining (IHC3+; n=9812 patients) and 25% based on FISH (n=681 patients). Data on the prevalence of ErbB2-positive breast cancer in Asia are limited. The current survey indicated that the prevalence in Asia may be similar to that in Western countries; thus, up to 1 in 4 Asian patients with breast cancer potentially could benefit from ErbB2-targeted treatment. A standard, reliable ErbB2 assessment method available to patients across Asia is urgently required.
    MeSH terms: Asia; Breast Neoplasms/genetics*; Breast Neoplasms/epidemiology*; Female; Humans; Immunohistochemistry; Prevalence; In Situ Hybridization, Fluorescence; Receptor, ErbB-2/genetics*
  20. Hairi NN, Bulgiba A, Cumming RG, Naganathan V, Mudla I
    BMC Public Health, 2010;10:492.
    PMID: 20716377 DOI: 10.1186/1471-2458-10-492
    The prevalence and correlates of physical disability and functional limitation among older people have been studied in many developed countries but not in a middle income country such as Malaysia. The present study investigated the epidemiology of physical disability and functional limitation among older people in Malaysia and compares findings to other countries.
    MeSH terms: Activities of Daily Living*; Aged; Cross-Sectional Studies; Disability Evaluation*; Female; Disabled Persons/classification*; Disabled Persons/statistics & numerical data; Humans; Malaysia; Male; Middle Aged; Rural Population; Social Class; Prevalence
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