Browse publications by year: 2013

  1. Khan AA, Asari MA, Hassan A, Aiman N
    Folia Morphol (Warsz), 2013 Feb;72(1):82-5.
    PMID: 23749717
    Anatomical variations in the femoral vein are of great clinical importance especially in cases of deep vein thrombosis (DVT). Knowledge of the variable anatomy of the femoral vein is important to minimise false-negative findings on ultrasound examination in patients with DVT and help to explain the 'silent' DVT. Furthermore, the presence of a duplicated femoral vein itself is associated with higher incidence of DVT. These venous anomalies are usually due to the truncular venous malformation. In the present study, while dissecting the right lower limb, we found a case of variation of the femoral vein. In this case, besides a duplicated femoral vein, we also noticed a 3rd interconnecting channel near the apex of the femoral triangle joining the two veins. This variation has not been reported previously by other authors. Considering its uniqueness and clinical importance, we decided to report this case.
    MeSH terms: Adult; Cadaver; Dissection; Femoral Vein/abnormalities*; Femoral Vein/anatomy & histology*; Humans; Leg/anatomy & histology; Leg/blood supply; Male; Venous Thrombosis/pathology*; Vascular Malformations/pathology*
  2. Farshad Ashraf M, Abd Aziz M, Abdul Kadir M, Stanslas J, Farokhian E
    Plant Cell Physiol, 2013 Aug;54(8):1356-64.
    PMID: 23749812 DOI: 10.1093/pcp/pct083
    This study focuses on the establishment of in vitro tuberization of Chlorophytum borivilianum using solid and liquid culture systems. A high in vitro tuberization rate on solid and stationary liquid Murashige and Skoog media was observed in the presence of 60 g l⁻¹ sucrose with 950, 1,265 and 1,580 µM 2-chloroethyl-trimethylammonium chloride (CCC). Application of a higher sucrose concentration of 90 g l⁻¹ showed a negative interaction with CCC on in vitro tuber number and days to in vitro tuber induction. For economic feasibility, 950 µM CCC with 60 g l⁻¹ sucrose was chosen as the best combination for in vitro tuberization in both solid and stationary liquid media. For optimization of in vitro tuber production,a comparison between solid, stationary liquid and shake liquid culture was carried out. Liquid culture with shaking at 80 r.p.m. resulted in a >2.5-fold increase in in vitro tuber production compared with solid culture.
    MeSH terms: Chlormequat/pharmacology*; Plant Growth Regulators/pharmacology*; Sucrose/pharmacology*; Plant Shoots/drug effects; Plant Shoots/growth & development; Liliaceae/drug effects*; Liliaceae/growth & development; Plant Tubers/drug effects*; Plant Tubers/growth & development; Culture Techniques/methods*
  3. Abdul Rahman WF, Md Hashim MN, Win TT, Bakrin IH
    BMJ Case Rep, 2013;2013.
    PMID: 23749834 DOI: 10.1136/bcr-2013-010001
    Solid variant of papillary thyroid carcinoma (PTC) is a rare, poorly characterised variant and predominantly reported in children with a history of radiation exposure. This variant has a high propensity for extra-thyroidal extension and cervical lymph node metastases. A 14-year-old Malay girl who had no history of radiation exposure, presented with multiple cervical lymphadenopathy and it was clinically suspicious for tuberculosis or lymphoma. An incisional biopsy revealed a metastatic PTC. The patient underwent total thyroidectomy with bilateral lateral neck dissection and histopathology report was solid variant of PTC. Whole-body I(131) scan was performed which revealed an intense tracer uptake in the neck. She was planned for radioactive iodine ablation and now on regular follow-up for monitoring of possible tumour metastasis.
    MeSH terms: Adolescent; Carcinoma/diagnosis*; Carcinoma/pathology; Diagnosis, Differential; Female; Humans; Lymphatic Diseases/diagnosis; Neck; Thyroid Neoplasms/diagnosis*; Thyroid Neoplasms/pathology
  4. Razmara J, Deris SB, Illias RB, Parvizpour S
    Bioinformation, 2013;9(7):345-8.
    PMID: 23750078 DOI: 10.6026/97320630009345
    A hidden Markov model (HMM) has been utilized to predict and generate artificial secretory signal peptide sequences. The strength of signal peptides of proteins from different subcellular locations via Lactococcus lactis bacteria correlated with their HMM bit scores in the model. The results show that the HMM bit score +12 are determined as the threshold for discriminating secreteory signal sequences from the others. The model is used to generate artificial signal peptides with different bit scores for secretory proteins. The signal peptide with the maximum bit score strongly directs proteins secretion.
  5. Jan Mohamed HJ, Mitra AK, Zainuddin LR, Leng SK, Wan Muda WM
    Women Health, 2013;53(4):335-48.
    PMID: 23751089 DOI: 10.1080/03630242.2013.788120
    Metabolic syndrome has been associated with an increased risk of cardiovascular disease and diabetes mellitus. The objective of this study was to determine gender differences in the prevalence and factors associated with metabolic syndrome in a rural Malay population. This cross-sectional study, conducted in Bachok, Kelantan, involved 306 respondents aged 18 to 70 years. The survey used a structured questionnaire to collect information on demographics, lifestyle, and medical history. Anthropometric measurements, such as weight, height, body mass index, waist and hip circumference, and blood pressure were measured. Venous blood samples were taken by a doctor or nurses and analyzed for lipid profile and fasting glucose. The overall prevalence of metabolic syndrome was 37.5% and was higher among females (42.9%). Being unemployed or a housewife and being of older age were independently associated with metabolic syndrome in a multivariate analysis. Weight management and preventive community-based programs involving housewives, the unemployed, and adults of poor education must be reinforced to prevent and manage metabolic syndrome effectively in adults.
    MeSH terms: Adolescent; Adult; Aged; Anthropometry; Blood Glucose/metabolism; Blood Pressure; Cardiovascular Diseases/complications; Cardiovascular Diseases/etiology; Cardiovascular Diseases/epidemiology; Cross-Sectional Studies; Female; Health Surveys; Humans; Hypertension/complications; Hypertension/epidemiology; Lipids/blood; Malaysia/epidemiology; Male; Middle Aged; Population Surveillance; Surveys and Questionnaires; Regression Analysis; Risk Factors; Rural Population/statistics & numerical data; Sex Factors; Socioeconomic Factors; Body Mass Index; Prevalence; Metabolic Syndrome X/complications; Metabolic Syndrome X/epidemiology*; Metabolic Syndrome X/physiopathology; Young Adult; Obesity, Abdominal/blood; Obesity, Abdominal/epidemiology*
  6. Tan PY, Tan CP, Abas F, Ho CW, Mustapha WA
    Molecules, 2013 Jun 10;18(6):6792-803.
    PMID: 23752466 DOI: 10.3390/molecules18066792
    Palm sugar-like flavouring (PSLF) is a type of flavour product that is formed by heating amino acids and sugar under specific heating conditions. Unfortunately, PSLF has a salty taste and contains high amounts of acrylamide. Hence, the objective of this research was to reduce saltiness and acrylamide without negatively affecting the aroma properties of PSLF. A decrease in the sodium phosphate (NaHPO₄) buffer concentration from 0.20 to 0.02 M was found to reduce sodium to approximately 15% of the level found in original PSLF. A further decrease (~25%) in the sodium content was achieved by removing monobasic sodium phosphate (NaH₂PO₄) from the buffer system. Meanwhile, the addition of CaCl₂ at 20-40 mg/L reduced the acrylamide content in PSLF by as much as 58%. A CaCl₂ concentration of 20 mg/mL was most favourable as it most efficiently suppressed acrylamide formation while providing an acceptably high flavour yield in PSLF. In view of the high acrylamide content in PSLF, additional work is necessary to further reduce the amount of acrylamide by controlling the asparagine concentration in the precursor mixture.
    MeSH terms: Amino Acids/chemistry; Buffers; Calcium Chloride/chemistry*; Carbohydrates/chemistry; Flavoring Agents/chemistry*; Hot Temperature; Phosphates/chemistry; Pyrazines/chemistry; Taste*; Acrylamide/chemistry*
  7. Mehrali M, Shirazi FS, Mehrali M, Metselaar HS, Kadri NA, Osman NA
    J Biomed Mater Res A, 2013 Oct;101(10):3046-57.
    PMID: 23754641 DOI: 10.1002/jbm.a.34588
    Functionally graded material (FGM) is a heterogeneous composite material including a number of constituents that exhibit a compositional gradient from one surface of the material to the other subsequently, resulting in a material with continuously varying properties in the thickness direction. FGMs are gaining attention for biomedical applications, especially for implants, owing to their reported superior composition. Dental implants can be functionally graded to create an optimized mechanical behavior and achieve the intended biocompatibility and osseointegration improvement. This review presents a comprehensive summary of biomaterials and manufacturing techniques researchers employ throughout the world. Generally, FGM and FGM porous biomaterials are more difficult to fabricate than uniform or homogenous biomaterials. Therefore, our discussion is intended to give the readers about successful and obstacles fabrication of FGM and porous FGM in dental implants that will bring state-of-the-art technology to the bedside and develop quality of life and present standards of care.
    MeSH terms: Biocompatible Materials/pharmacology*; Biocompatible Materials/chemistry*; Humans; Materials Testing; Dental Implants*; Dental Prosthesis Design; Mechanical Phenomena
  8. Maxwell O, Wagiran H, Ibrahim N, Lee SK, Sabri S
    Radiat Prot Dosimetry, 2013 Dec;157(2):271-7.
    PMID: 23754832 DOI: 10.1093/rpd/nct140
    The purpose of this project is to evaluate the suitability of different sites as locations for obtaining underground water for consumption. The analysis of ²³⁸U, ²³²Th and ⁴⁰K from rock samples from each layer of borehole at a depth of ∼50 m at Site A borehole, S3L1-S3L6 in Gosa and 40 m at Site B borehole, S4L1-S4L5 in Lugbe, Abuja, north central Nigeria is presented. The gamma-ray spectrometry was carried out using a high-purity germanium detector coupled to a computer-based high-resolution multichannel analyzer. The activity concentrations at Site A borehole for ²³⁸U have a mean value of 26 ± 3, ranging from 23 ± 2 to 30 ± 3 Bq kg⁻¹, ²³²Th a mean value of 63 ± 5, ranging from 48 ± 4 to 76 ± 6 Bq kg⁻¹ and ⁴⁰K a mean value of 573 ± 72, ranging from 437 ± 56 to 821 ± 60 Bq kg⁻¹. The activity concentrations at Site B borehole for ²³⁸U have a mean value of 20 ± 2, ranging from 16 ± 2 to 23 ± 2 Bq kg⁻¹, ²³²Th a mean value of 46 ± 4, ranging from 43 ± 4 to 49 ± 4 Bq kg⁻¹, ⁴⁰K a mean value of 915 ± 116 and ranging from 817 ± 103 Bq kg⁻¹ to 1011 ± 128 Bq kg⁻¹. It is noted that the higher activity concentrations of ²³²Th and ²³⁸U are found in Site A at Gosa. Site B has lower radioactivity, and it is recommended that both sites are suitable for underground water consumption.
    MeSH terms: Background Radiation*; Germanium; Humans; Nigeria; Potassium Radioisotopes/analysis*; Radiation Monitoring*; Soil Pollutants, Radioactive/analysis*; Spectrometry, Gamma; Thorium/analysis*; Uranium/analysis*
  9. Yen FY, Chong KM, Ha LM
    PLoS One, 2013;8(6):e65440.
    PMID: 23755231 DOI: 10.1371/journal.pone.0065440
    This paper proposes three synthetic-type control charts to monitor the mean time-between-events of a homogenous Poisson process. The first proposed chart combines an Erlang (cumulative time between events, Tr ) chart and a conforming run length (CRL) chart, denoted as Synth-Tr chart. The second proposed chart combines an exponential (or T) chart and a group conforming run length (GCRL) chart, denoted as GR-T chart. The third proposed chart combines an Erlang chart and a GCRL chart, denoted as GR-Tr chart. By using a Markov chain approach, the zero- and steady-state average number of observations to signal (ANOS) of the proposed charts are obtained, in order to evaluate the performance of the three charts. The optimal design of the proposed charts is shown in this paper. The proposed charts are superior to the existing T chart, Tr chart, and Synth-T chart. As compared to the EWMA-T chart, the GR-T chart performs better in detecting large shifts, in terms of the zero- and steady-state performances. The zero-state Synth-T4 and GR-Tr (r = 3 or 4) charts outperform the EWMA-T chart for all shifts, whereas the Synth-Tr (r = 2 or 3) and GR-T 2 charts perform better for moderate to large shifts. For the steady-state process, the Synth-Tr and GR-Tr charts are more efficient than the EWMA-T chart in detecting small to moderate shifts.
    MeSH terms: Accidents, Occupational/statistics & numerical data; Algorithms*; Ambulatory Care/statistics & numerical data; Coal Mining; Humans; Markov Chains*; Time; Models, Statistical*; Poisson Distribution
  10. Saharudin KA, Sreekantan S, Abd Aziz SN, Hazan R, Lai CW, Mydin RB, et al.
    J Nanosci Nanotechnol, 2013 Mar;13(3):1696-705.
    PMID: 23755576
    The present study deals with surface modification of Ti6Al4V alloy via anodization technique. The morphology, structure, adhesion and bioactivity of Ti6Al4V alloy after anodization process were investigated in detail. The influence of fluoride content and direct circuit (DC) applied voltage during anodization of Ti6Al4V alloy in a bath with electrolytes composed of ethylene glycol (EG) and ammonium fluoride (NH4F) were considered. It was found that the average pore sizes and length of nanoporous or nanotubes were increasing with the fluoride content and applied voltage. A minimum of 3 wt% of NH4F is required to grow a self-organized nanotube arrays. As the fluoride content was increased to 5 wt%, TiO2 nanotubes with average diameter of 110 nm and 3.4 microm lengths were successfully synthesized. It is noteworthy to point out that the rate of the nanotube formation was increasing up to 9 microm thick bioactive TiO2 nanotubes layer as anodization time was increased to 3 h. Based on the results obtained, the PA6 cells cultured on anodic Ti6Al4V alloy showed highest level of cell viability and greater cell adhesion compared to the flat Ti6Al4V foil substrate. In fact, highly ordered nanotubes structure on Ti6Al4V alloy can provide beneficial effects for PA6 cells in attachment and proliferation.
    MeSH terms: Cell Adhesion; Electrodes*; Microscopy, Electron, Scanning; Surface Properties; Titanium/pharmacology*; Crystallography, X-Ray; Nanotubes; Cell Proliferation; Photoelectron Spectroscopy
  11. Al-Khatib AR, Rajion ZA, Masudi SM, Hassan R, Townsend GC
    Homo, 2013 Aug;64(4):296-311.
    PMID: 23755965 DOI: 10.1016/j.jchb.2013.04.002
    The aim of this study was to evaluate the relationships of selected facial measurements with mesio-distal crown widths and dental arch dimensions in individuals with normal occlusions. A cross-sectional study was conducted on 276 subjects with Angle's Class I normal occlusions. Three-dimensional images of the face and dental casts were captured and analyzed using stereophotogrammetric systems. Significant correlations were found between the sagittal facial variables and both upper and lower dental arch dimensions and to lesser degree with the horizontal and vertical variables. The values of correlation coefficients calculated between facial and dental crown measurements ranged from .01 to .50 for upper teeth and .01 to .49 for lower teeth. The values of correlation coefficients between facial and upper dental arch dimensions ranged from .01 to .55 and those between facial and lower dental arch dimensions ranged from .01 to .60. A principal components analysis showed that the sagittal dimensions, face height, nose, labial fissure, binocular widths were positively associated with dental arch dimensions and mesio-distal crown diameters in males. On the other hand, only the sagittal variables were associated with dental dimensions in females. The results of this study confirm that positive associations exist between facial and dental arch dimensions. These relationships should be taken into consideration when attempts are made to modify dental arch size as part of orthodontic treatment. Moreover, these relationships are also relevant to prosthodontists involved with selecting tooth sizes that display optimal functional balance with the craniofacial structures.
    MeSH terms: Adolescent; Adult; Cross-Sectional Studies; Dental Arch/anatomy & histology; Dental Occlusion*; Face/anatomy & histology*; Female; Humans; Malaysia; Male; Tooth/anatomy & histology*; Imaging, Three-Dimensional; Young Adult
  12. Shueh CS, Neela V, Hussin S, Hamat RA
    J Microbiol Methods, 2013 Aug;94(2):141-143.
    PMID: 23756145 DOI: 10.1016/j.mimet.2013.06.001
    We developed a time-saving and cost-efficient Pulsed Field Gel Electrophoresis (PFGE) method for the typing of Stenotrophomonas maltophilia by modifying the conventional procedures. Our modifications related to the cell suspension preparation, lysis of bacterial cells in plugs, washing steps, and consumption of restriction enzyme. Although few rapid PFGE protocols on Gram-negative bacteria are available, the use of comparatively large amounts of costly reagents prompted us to look for other alternative. Hence, by considering the speed, simplicity, and relatively low cost, the modified protocol may be of more practical value than other established protocols in investigating S. maltophilia nosocomial outbreaks.
    MeSH terms: Humans; Bacterial Typing Techniques/economics; Bacterial Typing Techniques/methods*; Electrophoresis, Gel, Pulsed-Field/economics; Electrophoresis, Gel, Pulsed-Field/methods*; Gram-Negative Bacterial Infections/microbiology*; Stenotrophomonas maltophilia/classification; Stenotrophomonas maltophilia/genetics; Stenotrophomonas maltophilia/isolation & purification*
  13. Bhoo-Pathy N, Uiterwaal CS, Dik VK, Jeurnink SM, Bech BH, Overvad K, et al.
    Clin Gastroenterol Hepatol, 2013 Nov;11(11):1486-92.
    PMID: 23756220 DOI: 10.1016/j.cgh.2013.05.029
    BACKGROUND & AIMS: Few modifiable risk factors have been implicated in the etiology of pancreatic cancer. There is little evidence for the effects of caffeinated coffee, decaffeinated coffee, or tea intake on risk of pancreatic cancer. We investigated the association of total coffee, caffeinated coffee, decaffeinated coffee, and tea consumption with risk of pancreatic cancer.

    METHODS: This study was conducted within the European Prospective Investigation into Nutrition and Cancer cohort, comprising male and female participants from 10 European countries. Between 1992 and 2000, there were 477,312 participants without cancer who completed a dietary questionnaire and were followed up to determine pancreatic cancer incidence. Coffee and tea intake was calibrated with a 24-hour dietary recall. Adjusted hazard ratios (HRs) were computed using multivariable Cox regression.

    RESULTS: During a mean follow-up period of 11.6 y, 865 first incidences of pancreatic cancers were reported. When divided into fourths, neither total intake of coffee (HR, 1.03; 95% confidence interval [CI], 0.83-1.27; high vs low intake), decaffeinated coffee (HR, 1.12; 95% CI, 0.76-1.63; high vs low intake), nor tea were associated with risk of pancreatic cancer (HR, 1.22, 95% CI, 0.95-1.56; high vs low intake). Moderately low intake of caffeinated coffee was associated with an increased risk of pancreatic cancer (HR, 1.33; 95% CI, 1.02-1.74), compared with low intake. However, no graded dose response was observed, and the association attenuated after restriction to histologically confirmed pancreatic cancers.

    CONCLUSIONS: Based on an analysis of data from the European Prospective Investigation into Nutrition and Cancer cohort, total coffee, decaffeinated coffee, and tea consumption are not related to the risk of pancreatic cancer.

    MeSH terms: Coffee/adverse effects*; Diet/adverse effects*; Diet/methods*; Feeding Behavior; Female; Follow-Up Studies; Humans; Male; Middle Aged; Pancreatic Neoplasms/epidemiology*; Prospective Studies; Surveys and Questionnaires; Tea/adverse effects*; Cohort Studies; Risk Assessment
  14. Kavitha R, Nazni WA, Tan TC, Lee HL, Azirun MS
    J Forensic Leg Med, 2013 Jul;20(5):480-2.
    PMID: 23756518 DOI: 10.1016/j.jflm.2013.03.007
    Forensic entomological specimens collected from human decedents during crime scene investigations in Malaysia in the past 6 years (2005-2010) are reviewed. A total of 80 cases were recorded and 93 specimens were collected. From these specimens, 10 species of cyclorrphagic flies were identified, consisting of Chrysomya rufifacies (Macquart) -38 specimens (40.86%), Chrysomya megacephala (Fabricius) -36 specimens (38.70%), Chrysomya villeneuvi (Patton) -2 specimens (2.15%), Chrysomya nigripes (Aubertin) -2 specimens (2.15%), Chrysomya pinguis (Walker) -1 specimen (1.08%), Hermetia illucens (Linnaeus) -1 specimen (1.08%), Hemipyrellia liguriens (Wiedemann) -5 specimens (5.37%), Synthesiomyia nudiseta (Wulp) -1 specimen (1.08%), Megaselia scalaris (Loew)-1 specimen (1.08%) and Sarcophaga ruficornis (Fabricius) -4 specimens (4.30%). In two specimens (2.15%), the maggots were not identifiable. Ch. megacephala and Ch. rufifacies were the commonest species found in human decedents from three different ecological habitats. S. nudiseta is an uncommon species found only on human cadavers from indoors. A total of 75 cases (93.75%) had a single fly infestation and 5 cases (6.25%) had double fly infestation. In conclusion, although large numbers of fly species were found on human decedents, the predominant species are still those of Chrysomya.
    MeSH terms: Animals; Diptera/physiology*; Entomology; Feeding Behavior*; Humans; Larva; Malaysia; Postmortem Changes*; Forensic Pathology
  15. Gouk SW, Cheng SF, Mok JS, Ong AS, Chuah CH
    Br J Nutr, 2013 Dec 14;110(11):1987-95.
    PMID: 23756564 DOI: 10.1017/S0007114513001475
    The present study aimed to determine the effect of positional distribution of long-chain SFA in TAG, especially at the sn-1, 3 positions, on fat deposition using the C57BL/6 mouse model. Throughout the 15 weeks of the study, mice were fed with diets fortified with palm olein (POo), chemically interesterified POo (IPOo) and soyabean oil (SOY). Mice receiving the SOY-enriched diet gained significantly higher amounts of subcutaneous fat (P= 0·011) and total fat (P= 0·013) compared with the POo group, despite similar body mass gain being recorded. During normalisation with food consumption to obtain the fat:feed ratio, mice fed with the POo-enriched diet exhibited significantly lower visceral (P= 0·044), subcutaneous (P= 0·006) and total (P= 0·003) fat:feed than those fed with the SOY-enriched diet. It is noteworthy that mice fed with the IPOo-enriched diet gained 14·3 % more fat per food consumed when compared with the POo group (P= 0·013), despite their identical total fatty acid compositions. This was mainly attributed to the higher content of long-chain SFA at the sn-1, 3 positions of TAG in POo, which results in delayed absorption after deacylation as evidenced by the higher amounts of long-chain SFA excreted in the faeces of mice fed with the POo-enriched diet. Negative correlations were found between the subcutaneous, visceral as well as total fat accretion per food consumption and the total SFA content at the sn-1, 3 positions, while no relationships were found for MUFA and PUFA. The present results show that the positional distribution of long-chain SFA exerts a more profound effect on body fat accretion than the total SFA content.
    MeSH terms: Adipose Tissue/growth & development; Adipose Tissue/metabolism*; Animals; Diet, Reducing*; Dietary Fats/analysis; Dietary Fats/metabolism*; Digestion; Fatty Acids/analysis; Fatty Acids/metabolism*; Fatty Acids/chemistry; Feces/chemistry; Intestinal Absorption; Isomerism; Male; Mice, Inbred C57BL; Plant Oils/metabolism; Plant Oils/chemistry; Random Allocation; Soybean Oil/metabolism; Soybean Oil/chemistry; Triglycerides/metabolism*; Triglycerides/chemistry; Weaning; Molecular Structure; Weight Gain; Subcutaneous Fat/growth & development; Subcutaneous Fat/metabolism; Adiposity*; Subcutaneous Fat, Abdominal/growth & development; Subcutaneous Fat, Abdominal/metabolism; Mice
  16. Sow AY, Ismail A, Zulkifli SZ
    Environ Sci Pollut Res Int, 2013 Dec;20(12):8964-73.
    PMID: 23757028 DOI: 10.1007/s11356-013-1857-9
    The present study investigates the concentration of Pb, Cd, Ni, Zn, and Cu in the paddy field soils collected from Tumpat, Kelantan. Soil samples were treated with sequential extraction to distinguish the anthropogenic and lithogenic origin of Pb, Cd, Ni, Zn, and Cu. ELFE and oxidizable-organic fractions were detected as the lowest accumulation of Pb, Cd, Ni, Zn, and Cu. Therefore, all the heavy metals examined were concentrated, particularly in resistant fraction, indicating that those heavy metals occurred and accumulated in an unavailable form. The utilization of agrochemical fertilizers and pesticides might not elevate the levels of heavy metals in the paddy field soils. In comparison, the enrichment factor and geoaccumulation index for Pb, Cd, Ni, Zn, and Cu suggest that these heavy metals have the potential to cause environmental risk, although they present abundance in resistant fraction. Therefore, a complete study should be conducted based on the paddy cycle, which in turn could provide a clear picture of heavy metals distribution in the paddy field soils.
    MeSH terms: Agriculture; Environmental Monitoring/methods*; Environmental Pollution/statistics & numerical data*; Fertilizers; Malaysia; Oryza; Soil/chemistry; Soil Pollutants/analysis*; Metals, Heavy/analysis*
  17. Dewi FD, Gundavarapu KC, Cugati N
    Oral Health Prev Dent, 2013;11(2):131-9.
    PMID: 23757453 DOI: 10.3290/j.ohpd.a29735
    PURPOSE: To find the differences in patient satisfaction related to dental services among three ethnic groups - Chinese, Indian and Malay - at AIMST University Dental Centre and analyse them with an importance-performance grid, identifying the weak and strong points, in order to provide better service.

    MATERIALS AND METHODS: This questionnaire-based study consisted of convenience samples of 174 patients of Chinese, Indian and Malay ethnicity. Importance-performance analysis for 20 attributes were compared using Likert's scale. The data obtained were statistically analysed using the Kruskal-Wallis test.

    RESULTS: Chinese and Indians both emphasised low performance on the interpersonal relationship attribute in terms of the receptionist's courtesy, whereas the Malay participants were concerned with convenience attributes. All the ethnic groups favoured maintaining existing major attributes towards technical competency, interpersonal relationship and facility factors. This study demonstrated priority differences between the ethnic groups' perception of the quality of dental services, where ethnic Chinese showed the highest gap (measure of dissatisfaction) between importance and performance compared to ethnic Malays, followed by ethnic Indians.

    CONCLUSION: The patients from the three major ethnic groups of Malaysia were generally well satisfied. Perhaps more priority should be placed on improving the interpersonal relationship attribute, especially with the receptionists.

    MeSH terms: Adolescent; Adult; Attitude to Health; Child; China/ethnology; Clinical Competence; Dental Auxiliaries; Dental Care/psychology*; Dental Care/standards; Dental Clinics/organization & administration; Dental Health Services/standards; Dentist-Patient Relations; Educational Status; Ethnic Groups/ethnology; Ethnic Groups/psychology*; Female; Humans; India/ethnology; Malaysia; Male; Middle Aged; Professional-Patient Relations; Quality Assurance, Health Care/methods; Quality of Health Care; Surveys and Questionnaires; Patient Satisfaction*; Marital Status; Young Adult
  18. Jayachandra S, D'Souza UJ
    PMID: 23758154
    The objective of this study was to study the possible reproductive adverse effects of the diazinon on rat offspring exposed in utero and during lactation. Dams were gavaged daily (10, 15, and 30 mg/kg) before mating, during mating, and during pregnancy and lactation in separate groups. Reproductive outcome data of dams were examined. Body weight, testis weight, testicular marker enzyme activities (alkaline phosphatase, acid phosphatase, lactate dehydrogenase, and glucose-6-phosphate dehydrogenase), qualitative and quantitative testicular and epididymal histology, and immunohistochemisty for 3-β-hydroxysteroid dehydrogenase (HSD) were examined in male offspring at puberty and adulthood. The 30-mg/kg dose induced significant adverse effects at both puberty and adulthood in offspring. At puberty the male offspring showed a decrease in testicular weight, degenerative changes, and 3-β-HSD. Moreover, an increase in activity of alkaline and acid phosphatase also was observed. At adulthood, there was a decrease in testicular weight and 3-β-HSD with an increase in the levels of testicular marker enzyme. There was evidence of some adverse reproductive effects in male offspring at the 15-mg/kg dose. Most of the adverse effects were irreversible and were evident at both puberty and adulthood in offspring, although a few parameters reverted back to the normal growth pattern. Hence, diazinon is a reproductive toxicant in male offspring, which caused significant damage to the testes when exposed during prenatal and postnatal life.
    MeSH terms: Animals; Animals, Newborn/growth & development; Animals, Newborn/metabolism*; Diazinon/pharmacology*; Diazinon/toxicity; Dose-Response Relationship, Drug; Epididymis/drug effects; Epididymis/pathology; Female; Insecticides/pharmacology*; Insecticides/toxicity; Male; Organ Size/drug effects; Pregnancy; Pregnancy Outcome; Prenatal Exposure Delayed Effects/chemically induced*; Prenatal Exposure Delayed Effects/metabolism; Prenatal Exposure Delayed Effects/pathology; Spermatogenesis/drug effects*; Spermatogenesis/physiology; Spermatozoa/drug effects*; Spermatozoa/pathology; Testis/drug effects; Testis/enzymology*; Testis/pathology; 3-Hydroxysteroid Dehydrogenases/metabolism; Biomarkers/metabolism; Rats, Sprague-Dawley; Models, Animal; Rats
  19. Kew ST, Chakravarthi S
    N Engl J Med, 2013 Jun 13;368(24):2303.
    PMID: 23758235 DOI: 10.1056/NEJMicm1204882
    MeSH terms: Cascara/adverse effects; Colon/pathology*; Colonic Diseases/chemically induced; Colonic Diseases/pathology*; Female; Humans; Melanosis/chemically induced; Melanosis/pathology*; Middle Aged
  20. Hussein AS, Ghasheer HF, Ramli NM, Schroth RJ, Abu-Hassan MI
    Eur J Paediatr Dent, 2013 Jun;14(2):113-8.
    PMID: 23758460
    AIM: To assess the salivary levels of Copper (Cu), Zinc (Zn), Manganese (Mn) and Iron (Fe) obtained from children of different ethnic backgrounds in Shah Alam, Malaysia and investigate the possible relationships with caries.

    MATERIALS AND METHODS: One hundred and twenty primary school children were included. They were divided into caries and caries-free groups. Unstimulated whole saliva was collected from each participant using spitting method. The salivary elements were measured using an Atomic Absorption Spectrophotometer. Descriptive statistics, bivariate and Pearson's correlation analysis were performed.

    RESULTS: Salivary Cu and Zn levels were significantly higher in children with dental caries compared to those caries-free (p < 0.05). Moreover, these elements had a positive correlation with dental caries (Cu: r=0.698, p<0.001; Zn: r=0.181, p<0.05). No significant variations in Mn and Fe were observed between caries and caries-free group (p>0.05). Additionally, there were significant differences in salivary Zn and Fe among different age groups (p<0.05) and highly significant differences in salivary Cu, Mn and Fe among different ethnic groups (p<0.001). However, all elements exhibited no significant differences between males and females.

    CONCLUSION: The salivary Cu and Zn levels showed significant differences between caries and caries-free groups. The findings also revealed significant variations in the levels of salivary Cu, Mn and Fe among different ethnic groups and salivary Zn and Fe among different age groups.

    MeSH terms: Age Factors; Child; China/ethnology; Copper/analysis; Dental Caries/ethnology; Dental Caries/metabolism*; DMF Index; Ethnic Groups*; Female; Humans; India/ethnology; Iron/analysis; Malaysia; Male; Manganese/analysis; Saliva/chemistry*; Sex Factors; Spectrophotometry, Atomic; Trace Elements/analysis*; Zinc/analysis
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