Browse publications by year: 2013

  1. Haafiz MK, Hassan A, Zakaria Z, Inuwa IM, Islam MS, Jawaid M
    Carbohydr Polym, 2013 Oct 15;98(1):139-45.
    PMID: 23987327 DOI: 10.1016/j.carbpol.2013.05.069
    In this work, polylactic acid (PLA) composites filled with microcrystalline cellulose (MCC) from oil palm biomass were successfully prepared through solution casting. Fourier transform infrared (FT-IR) spectroscopy indicates that there are no significant changes in the peak positions, suggesting that incorporation of MCC in PLA did not result in any significant change in chemical structure of PLA. Thermogravimetric analysis was conducted on the samples. The T50 decomposition temperature improved with addition of MCC, showing increase in thermal stability of the composites. The synthesized composites were characterized in terms of tensile properties. The Young's modulus increased by about 30%, while the tensile strength and elongation at break for composites decreased with addition of MCC. Scanning electron microscopy (SEM) of the composites fractured surface shows that the MCC remained as aggregates of crystalline cellulose. Atomic force microscopy (AFM) topographic image of the composite surfaces show clustering of MCC with uneven distribution.
    MeSH terms: Cellulose/chemistry*; Fruit/chemistry; Polymers/chemistry*; Temperature; Biomass*; Lactic Acid/chemistry*; Arecaceae/chemistry*; Mechanical Processes
  2. Thomas R, Hamat RA, Neela V
    J Med Microbiol, 2013 Nov;62(Pt 11):1777-1779.
    PMID: 23988629 DOI: 10.1099/jmm.0.063230-0
    MeSH terms: Animals; Biological Assay; Disease Models, Animal*; Survival Analysis; Caenorhabditis elegans/microbiology*; Stenotrophomonas maltophilia/pathogenicity*
  3. Ali MS, Said ZS, Rahman RN, Chor AL, Basri M, Salleh AB
    Int J Mol Sci, 2013 Aug 28;14(9):17608-17.
    PMID: 23989606 DOI: 10.3390/ijms140917608
    Seeding is a versatile method for optimizing crystal growth. Coupling this technique with capillary counter diffusion crystallization enhances the size and diffraction quality of the crystals. In this article, crystals for organic solvent-tolerant recombinant elastase strain K were successfully produced through microseeding with capillary counter-diffusion crystallization. This technique improved the nucleation success rate with a low protein concentration (3.00 mg/mL). The crystal was grown in 1 M ammonium phosphate monobasic and 0.1 M sodium citrate tribasic dihydrate pH 5.6. The optimized crystal size was 1 × 0.1 × 0.05 mm³. Elastase strain K successfully diffracted up to 1.39 Å at SPring-8, Japan, using synchrotron radiation for preliminary data diffraction analysis. The space group was determined to be monoclinic space group P12(1)1 with unit cell parameters of a = 38.99 Ǻ, b = 90.173 Å and c = 40.60 Å.
    MeSH terms: Crystallization/methods*; Pancreatic Elastase/chemistry*; Pseudomonas aeruginosa/enzymology*; Solvents/chemistry*
  4. Ngeow YF, Leong ML, Wong YL, Wong GJ, Tan JL, Wee WY, et al.
    Genome Announc, 2013;1(4).
    PMID: 23990576 DOI: 10.1128/genomeA.00669-13
    Mycobacterium massiliense is a nontuberculous mycobacterium associated with human infections. We report here the draft genome sequence of M. massiliense strain M159, isolated from the bronchial aspirate of a patient who had a pulmonary infection. This strain showed genotypic and in vitro resistance to a number of tetracyclines and beta-lactam antibiotics.
    MeSH terms: Anti-Bacterial Agents; Humans; Monobactams; Nontuberculous Mycobacteria; Respiratory Tract Infections; Tetracyclines
  5. Chuah LO, Ho WY, Beh BK, Yeap SK
    PMID: 23990846 DOI: 10.1155/2013/751658
    Garcinia is a plant under the family of Clusiaceae that is commonly used as a flavouring agent. Various phytochemicals including flavonoids and organic acid have been identified in this plant. Among all types of organic acids, hydroxycitric acid or more specifically (-)-hydroxycitric acid has been identified as a potential supplement for weight management and as antiobesity agent. Various in vivo studies have contributed to the understanding of the anti-obesity effects of Garcinia/hydroxycitric acid via regulation of serotonin level and glucose uptake. Besides, it also helps to enhance fat oxidation while reducing de novo lipogenesis. However, results from clinical studies showed both negative and positive antiobesity effects of Garcinia/hydroxycitric acid. This review was prepared to summarise the update of chemical constituents, significance of in vivo/clinical anti-obesity effects, and the importance of the current market potential of Garcinia/hydroxycitric acid.
    MeSH terms: Citrates; Flavonoids; Flavoring Agents; Glucose; Obesity; Serotonin; Anti-Obesity Agents; Dietary Supplements; Clusiaceae; Garcinia; Lipogenesis; Phytochemicals
  6. Sady H, Al-Mekhlafi HM, Mahdy MA, Lim YA, Mahmud R, Surin J
    PLoS Negl Trop Dis, 2013;7(8):e2377.
    PMID: 23991235 DOI: 10.1371/journal.pntd.0002377
    BACKGROUND: Schistosomiasis, one of the most prevalent neglected tropical diseases, is a life-threatening public health problem in Yemen especially in rural communities. This cross-sectional study aims to determine the prevalence and associated risk factors of schistosomiasis among children in rural Yemen.

    METHODS/FINDINGS: Urine and faecal samples were collected from 400 children. Urine samples were examined using filtration technique for the presence of Schistosoma haematobium eggs while faecal samples were examined using formalin-ether concentration and Kato Katz techniques for the presence of S. mansoni. Demographic, socioeconomic and environmental information were collected via a validated questionnaire. Overall, 31.8% of the participants were found to be positive for schistosomiasis; 23.8% were infected with S. haematobium and 9.3% were infected with S. mansoni. Moreover, 39.5% of the participants were anaemic whereas 9.5% had hepatosplenomegaly. The prevalence of schistosomiasis was significantly higher among children aged >10 years compared to those aged ≤ 10 years (P<0.05). Multivariate analysis confirmed that presence of other infected family member (P<0.001), low household monthly income (P = 0.003), using unsafe sources for drinking water (P = 0.003), living nearby stream/spring (P = 0.006) and living nearby pool/pond (P = 0.002) were the key factors significantly associated with schistosomiasis among these children.

    CONCLUSIONS/SIGNIFICANCE: This study reveals that schistosomiasis is still highly prevalent in Yemen. These findings support an urgent need to start an integrated, targeted and effective schistosomiasis control programme with a mission to move towards the elimination phase. Besides periodic drug distribution, health education and community mobilisation, provision of clean and safe drinking water, introduction of proper sanitation are imperative among these communities in order to curtail the transmission and morbidity caused by schistosomiasis. Screening and treating other infected family members should also be adopted by the public health authorities in combating this infection in these communities.

    MeSH terms: Adolescent; Age Factors; Animals; Anthelmintics/therapeutic use; Child; Child, Preschool; Cross-Sectional Studies; Demography; Feces/parasitology; Female; Health Education; Humans; Infant; Infant, Newborn; Male; Surveys and Questionnaires; Risk Factors; Rural Population; Schistosoma haematobium/isolation & purification*; Schistosoma mansoni/isolation & purification*; Schistosomiasis/epidemiology*; Schistosomiasis/parasitology; Schistosomiasis/prevention & control; Socioeconomic Factors; Urine/parasitology; Yemen/epidemiology; Prevalence
  7. Delshad S, Chun JM
    Int J Ophthalmol, 2013;6(4):467-70.
    PMID: 23991380 DOI: 10.3980/j.issn.2222-3959.2013.04.11
    To describe and compare the corneal endothelial cell density and morphology in young, low and moderate myopic Chinese adults in Malaysian Chinese population.
  8. Liam CK, Pang YK, Poh ME, Kow KS, Wong CK, Varughese R
    Respirol Case Rep, 2013 Sep;1(1):20-2.
    PMID: 25473531 DOI: 10.1002/rcr2.14
    Breast metastases from non-small cell lung carcinoma are rarely reported. We report a case of a female patient with primary adenocarcinoma of the lower lobe of her right lung presenting with a massive right-sided malignant pleural effusion. The tumor harbored an epidermal growth factor receptor insertion mutation in exon 20 but was anaplastic lymphoma kinase translocation negative. She did not respond to treatment with erlotinib. First- and second-line cytotoxic chemotherapy resulted in stable disease as the best responses. She developed right breast metastasis 20 months after her initial presentation. The rarity of the condition and the likely mechanism of the breast metastasis are discussed.
    MeSH terms: Erlotinib Hydrochloride; Adenocarcinoma; Carcinoma, Non-Small-Cell Lung; Exons; Female; Humans; Lung Neoplasms; Receptor, Epidermal Growth Factor; Pleural Effusion, Malignant; Mutagenesis, Insertional; Receptor Protein-Tyrosine Kinases
  9. Abolfathi Momtaz Y, Hamid TA, Ibrahim R
    Psychogeriatrics, 2013 Sep;13(3):182-8.
    PMID: 25913768 DOI: 10.1111/psyg.12009
    Elder abuse is a pervasive phenomenon around the world with devastating effects on the victims. Although it is not a new phenomenon, interest in examining elder abuse is relatively new. This paper aims to provide an overview of the aetiological theories and measures of elder abuse. The paper briefly reviews theories to explain causes of elder abuse and then discusses the most commonly used measures of elder abuse. Based on the reviewed theories, it can be concluded that elder abuse is a multifactorial problem that may affect elderly people from different backgrounds and involve a wide variety of potential perpetrators, including caregivers, adult children, and partners. The review of existing measurement instruments notes that many different screening and assessment instruments have been developed to identify elders who are at risk for or are victims of abuse. However, there is a real need for more measurements of elder abuse, as the current instruments are limited in scope.
    MeSH terms: Aged; Aged, 80 and over; Elder Abuse*; Humans; Risk Factors; Caregivers*
  10. Mohammed Nawi A, Ismail A, Abdullah S
    Iran J Public Health, 2013 Sep;42(9):996-1006.
    PMID: 26060660
    BACKGROUND:
    Child with Down's syndrome is an individual who is suitable and eligible to receive early intervention services. This study aimed to measure the family outcome among parents of Down syndrome children, on the impact of receiving early intervention and identify the factors influencing it.

    METHODS:
    A cross sectional was conducted from April 2009 until January 2010 with a total of 125 parents of children with Down syndrome. There are five domains of family outcomes that has been studied which are understanding the strengths, abilities and special needs of children, knowing the rights and talk on children behalf, assisting the child to grow and learn, having a support system and be involved in the community. Children with Down syndrome aged four to 15 years was chosen as the respondents when they were accompanying their children in seven rehabilitation centers or during house visits.

    RESULTS:
    Family outcomes among parents of Down syndrome children who receive early intervention is better, 67.3 percent, compared to parents of Down syndrome children who receive late intervention, 41.4 percent. There are significant relationship between the acceptance level of intervention, parents education level, family income and the family outcomes. Parents of children who receive early intervention were more positive in understanding the strengths, abilities and special needs of their children compared to other family outcomes.

    CONCLUSION:
    Families whom children received early intervention had indirectly proved the importance and benefit of early intervention, not only for children with special needs, but for their family as well.

    KEYWORDS:
    Down syndrome children; intervention; special needs
    MeSH terms: Child; Cross-Sectional Studies; Down Syndrome; Humans; Income; Learning; Parents; Surveys and Questionnaires; Rehabilitation Centers; Early Intervention (Education); Knowledge
  11. Sahoo S, Myint KT, Soe HH, Singh S
    PMID: 26107029 DOI: 10.1097/APO.0b013e3182a6481a
    PURPOSE: One of the major challenges lies in the use of strategies to assess students working in small groups. The assessment by tutors and peers, and self-assessment by students appear to be integral parts of problem-based-learning (PBL) tutorials. Our study aimed to analyze the formative assessment scores by students themselves, their tutor and peers during undergraduate ophthalmology tutorials.

    DESIGN: A cross sectional study.

    METHODS: One hundred undergraduate students' self-assessment made during ophthalmology PBL tutorials were compared with that made by their tutor and student peers. At the end of every session, each student and student peers were given an assessment form dealing with criteria like responsibility, information processing, communication, critical analysis and self-awareness. The questionnaires were filled using the 5-point Likert scale. The tutor's assessment was made using the same criteria.

    RESULTS: There were significant positive correlations between self-assessment and tutor assessment in regard to participation in PBL session (P = 0.01), punctuality for each session (P ≤ 0.001) and bring new information (P = 0.001). There was a positive correlation between self-assessment and peer assessment regarding active participation (P = 0.02) and bringing new information (P = 0.003). However, there was no significant correlation between the overall scores given by selves and those by peers and the tutor.

    CONCLUSIONS: Students tended to overrate themselves. The self-assessment scores were generally higher than those given by their tutor and peers. To foster evidence-based practice of ophthalmology, the information acquisition skills need to be improved.

  12. Juhan N, Abd Razak N, Zubairi YZ, Khattak MN, Naing NN
    Iran J Public Health, 2013 Sep;42(9):980-7.
    PMID: 26175969
    BACKGROUND: Cervical cancer is the third most common cancer among women in Malaysia. The objective of this study was to estimate the effect of explanatory variables on survival time of cervical cancer patients receiving treatment at a hospital in Malaysia.

    METHODS: In this retrospective record review study, cervical cancer data obtained from Hospital UniversitiSains Malaysia (HUSM) was analysed. The data comprises of 120 patients who had been diagnosed as cervical cancer between 1(st) July 1995 and 30(th) June 2007, and obtained treatment from the hospital. The outcome variable was survival time (in months) from cervical cancer diagnosis to death. A stratified Weibull model was applied to study the effect of explanatory variable on survival time when there was time-dependent covariate in the model.

    RESULTS: Stage of disease and metastases were important prognostic variables. However, metastasis had been stratified because this variable did not satisfy the proportional hazard assumption. In without metastasis stratum, patients who were diagnosed at stage III & IV are at 2.30 times the risk of death as those in stage I & II. Meanwhile, in with metastasis stratum, patients in stage III & IV group had 3.53 times the hazard faced by patients in stage I & II.

    CONCLUSION: The prognosis of cervical cancer patients was dependent upon the stage at diagnosis, after the stratification of the metastasis variable. A poorer prognosis on survival was observed for patients in stage III & IV than those in stage I & II.

  13. Ahmad A, Hamid R, Dada AC, Usup G
    Probiotics Antimicrob Proteins, 2013 Sep;5(3):165-75.
    PMID: 26782985 DOI: 10.1007/s12602-013-9140-4
    Bacteriocin-producing Pseudomonas putida strain FStm2 isolated from shark showed broad range of antibacterial activity against all pathogens tested except Bacillus subtilis ATCC11774, MRSA N32064, Proteus mirabilis ATCC12453, Enterococcus faecalis ATCC14506, Salmonella typhimurium ATCC51312, Salmonella mutan ATCC25175, and Aeromonas hydrophila Wbf314. Of the three growth media tested in this study, TSB was observed to support the bacteriocin activity the most. While the highest bacteriocin activity was observed for media supplemented with 1 % NaCl, there was an observed reduction in bacteriocin activity with increasing salt concentration. Although the least bacteriocin activity was observed for marine broth, addition of increasing amounts of tryptone, glucose, or yeast extract increased bacteriocin activity. This was, however, contrary to the effect observed when MgSO4 and MnSO4 were added as supplements. In the presence of α-amylase, lipase, DNase, and RNase, a positive effect on bacteriocin production was observed. Proteinase K strongly inhibited bacteriocin production. Furthermore, the bacteriocins produced were heat stable within the temperature range of 30-70 °C. Bacteriocin activity also was not affected within a wide pH range of 3-9. Exposure to detergents did not inhibit the activity of the bacteriocin at the concentrations tested. Instead, a positive effect on the relative activity of produced bacteriocin was observed as sodium dodecyl sulfate (SDS), EDTA, and Tween 20 at 1 % concentration all improved bacteriocin activity when the cell-free supernatant was tested against Serratia marcescens ATCC 13880. The bacteriocin was purified by ammonium sulfate precipitation and gel filtration on a Superdex-200 column. SDS-PAGE analysis of the partially purified bacteriocin revealed an apparent molecular weight of ~32 kDa.
  14. Keshavarz S, Baharudin R, Mounts NS
    J Genet Psychol, 2013 May-Jun;174(3):253-70.
    PMID: 23991523
    The authors investigated the moderating role of father's education on the associations between perceived paternal parenting styles and locus of control among 382 Malaysian adolescents with an average age of 14.27. Data were collected by means of adolescents' self-report using standardized instruments (i.e., parental authority questionnaire and Nowicki-Strickland Internal-External Control Scale for Children). Results revealed that there were significant negative relationships between fathers' authoritative parenting style (r = -.243, p < .001) and authoritarian parenting style (r = -.130, p < .01) with adolescents' internal locus of control. Furthermore, the findings indicated that father's high level of education moderated the relationship between perceived paternal authoritarian parenting and locus of control (b = -0.147, p < .001). The findings underscore the need to include the role of parents' education when assessing the links between parenting styles and adolescents' locus of control.
    MeSH terms: Adolescent; Adult; Aged; Authoritarianism; Culture; Educational Status; Father-Child Relations*; Fathers/education; Fathers/psychology*; Female; Humans; Internal-External Control*; Malaysia/ethnology; Male; Middle Aged; Surveys and Questionnaires; Social Perception; Parenting/ethnology*; Parenting/psychology
  15. Nge CE, Gan CY, Low YY, Thomas NF, Kam TS
    Org. Lett., 2013 Sep 20;15(18):4774-7.
    PMID: 23991636 DOI: 10.1021/ol4021404
    Two new indole alkaloids, voatinggine (1) and tabertinggine (2), which are characterized by previously unencountered natural product skeletons, were isolated from a Malayan Tabernaemontana species. The structures and absolute configuration of these alkaloids were determined using NMR and MS analysis, and X-ray diffraction analysis. A possible biogenetic pathway to these novel alkaloids from an iboga precursor, and via a common cleavamine-type intermediate, is presented.
    MeSH terms: Stereoisomerism; Molecular Structure; Crystallography, X-Ray; Plant Leaves/chemistry; Nuclear Magnetic Resonance, Biomolecular; Indole Alkaloids/chemical synthesis*; Indole Alkaloids/chemistry; Tabernaemontana/chemistry*
  16. Ahmadian M, Samah AA
    Asian Pac J Cancer Prev, 2013;14(7):4005-13.
    PMID: 23991945
    BACKGROUND: Although breast cancer is a major public health worry among Asian women, adherence to screening for the disease remains an obstacle to its prevention. A variety of psycho-social and cultural factors predispose women to delay or avoidance of screening for breast cancer symptoms at the early stages when cure is most likely to be successful. Yet few interventions implemented to date to address this condition in this region have drawn on health behavior theory.

    MATERIALS AND METHODS: This paper reviews the existing literature on several cognitive theories and models associated with breast cancer screening, with an emphasis on the work that has been done in relation to Asian women. To conduct this review, a number of electronic databases were searched with context-appropriate inclusion criteria.

    RESULTS: Little empirical work was found that specifically addressed the applicability of health theories in promoting adherence to the current breast cancer prevention programs Among Asian women. However, a few studies were found that addressed individual cognitive factors that are likely to encourage women's motivation to protect themselves against breast cancer in this region of the world. The findings suggest that multi-level, socio-cultural interventions that focus on cognitive factors have much promise with this issue.

    CONCLUSIONS: Interventions are needed that effectively and efficiently target the personal motivation of at-risk Asian women to seek out and engage in breast cancer prevention. Concerning implications, personal motivation to seek out and engage in individual preventive actions for breast cancer prevention among Asian women is a timely, high priority target with practical implications for community development and health promotion. Further studies using qualitative, anthropologic approaches shaped for implementation in multi-ethnic Asian settings are needed to inform and guide these interventions.

    MeSH terms: Asia; Breast Neoplasms/diagnosis*; Breast Neoplasms/prevention & control; Breast Neoplasms/psychology; Female; Humans; Motivation; Health Behavior*; Early Detection of Cancer/psychology*
  17. Ho CC, Tan WP, Pathmanathan R, Tan WK, Tan HM
    Asian Pac J Cancer Prev, 2013;14(7):4057-9.
    PMID: 23991952
    BACKGROUND: Fluorescence in situ hybridization (FISH) testing may be useful to screen for bladder carcinoma or dysplasia by detecting aneuploidy chromosomes 3, 7, 17 and deletion of the chromosome 9p21 locus in urine specimens. This study aimed to assess the sensitivity, specificity, positive and negative predictive value of FISH in a multi-ethnic population in Asia.

    MATERIALS AND METHODS: Patients with haematuria and/or past history of urothelial cancer on follow-up had their voided urine tested with FISH. Patients then underwent cystoscopy/ ureteroscopy and any lesions seen were biopsied. The histopathological reports of the bladder or ureteroscopic mucosal biopsies were then compared with the FISH test results.

    RESULTS: Two hundred sixty patients were recruited. The sensitivity and specificity of the FISH test was 89.2% and 83.4% respectively. The positive (PPV) and negative predictive values (NPV) were 47.1% and 97.9%. By excluding patients who had positive deletion of chromosome 9, the overall results of the screening test improved: sensitivity 84.6%; specificity 96.4%; PPV 75.9% and NPV 97.9%.

    CONCLUSIONS: UroVysion FISH has a high specificity of detecting urothelial cancer or dysplasia when deletion of chromosome 9 is excluded. Negative UroVysion FISH-tests may allow us to conserve health resources and minimize trauma by deferring cystoscopic or ureteroscopic examination.

    MeSH terms: Adult; Aged; Aged, 80 and over; Urinary Bladder Neoplasms/diagnosis*; Cystoscopy/methods*; Female; Follow-Up Studies; Humans; Middle Aged; Prognosis; Retrospective Studies; Sensitivity and Specificity; In Situ Hybridization, Fluorescence/methods*; Ureteroscopy/methods*; Young Adult
  18. Abdull Razis AF, Noor NM
    Asian Pac J Cancer Prev, 2013;14(7):4235-8.
    PMID: 23991982
    Glucoraphanin is the main glucosinolate found in broccoli and other cruciferous vegetables (Brassicaceae). The objective of the study was to evaluate whether glucoraphanin and its breakdown product sulforaphane, are potent modulators of various phase I and phase II enzymes involved in carcinogen-metabolising enzyme systems in vitro. The glucosinolate glucoraphanin was isolated from cruciferous vegetables and exposed to human hepatoma cell line HepG2 at various concentrations (0-25 μM) for 24 hours. Glucoraphanin at higher concentration (25 μM) decreased dealkylation of methoxyresorufin, a marker for cytochrome P4501 activity; supplementation of the incubation medium with myrosinase (0.018 U), the enzyme that converts glucosinolate to its corresponding isothiocyanate, showed minimal induction in this enzyme activity at concentration 10 μM. Quinone reductase and glutathione S-transferase activities were unaffected by this glucosinolate; however, supplementation of the incubation medium with myrosinase elevated quinone reductase activity. It may be inferred that the breakdown product of glucoraphanin, in this case sulforaphane, is superior than its precursor in modulating carcinogen- metabolising enzyme systems in vitro and this is likely to impact on the chemopreventive activity linked to cruciferous vegetable consumption.
    MeSH terms: Glucosinolates/pharmacology*; Glutathione Transferase/metabolism*; Glycoside Hydrolases/metabolism*; Carcinoma, Hepatocellular/drug therapy; Carcinoma, Hepatocellular/enzymology*; Humans; Imidoesters/pharmacology*; Liver Neoplasms/drug therapy; Liver Neoplasms/enzymology; Tumor Cells, Cultured; Anticarcinogenic Agents/pharmacology; NAD(P)H Dehydrogenase (Quinone)/metabolism*; Isothiocyanates/pharmacology*
  19. Hasan SI, Mohd Ashari NS, Mohd Daud K, Che Husin CM
    Int J Rheum Dis, 2013 Aug;16(4):430-6.
    PMID: 23992264 DOI: 10.1111/1756-185X.12062
    BACKGROUND: The ethiopathogenesis of increased apoptosis of lymphocytes in systemic lupus erythematosus (SLE) is still incompletely understood but anti-C1q autoantibodies have been shown to induce apoptosis in lymphocytes from healthy donors and certain cell lines.
    AIM: This study was undertaken to investigate the relationship between peripheral lymphocyte apoptosis and serum levels of anti-C1q autoantibodies in SLE patients.
    METHODS: The sera of 124 patients with SLE involving 62 active SLE and 62 inactive SLE, fulfilling America College of Rheumatology (ACR) classification criteria for SLE (1997) were incubated with peripheral blood lymphocytes of healthy donors. The results were compared with 124 sex- and age-matched normal controls. Apoptotic lymphocytes (AL) were detected by flow cytometry using annexin V and propidium iodide binding. Anti-C1q autoantibodies were detected by an enzyme-linked immunoassay kit for all SLE patients.
    RESULTS: Results demonstrated that the percentage of AL in the peripheral blood of active SLE patients was significantly higher (n = 62, 34.95 ± 12.78%) than that of the inactive SLE patients (n = 62, 30.69 ± 10.13%, P = 0.042, 95%CI = 0.16-8.36) and normal controls (n = 124, 27.92 ± 10.22%, P = 0.001, 95%CI = 3.33-10.73). The percentage of AL significantly correlated with serum levels of anti-C1q autoantibodies in the active SLE patients (r = 0.263, P = 0.039) but not in the inactive SLE patients (r = 0.170, P = 0.185).
    CONCLUSION: The results of this study suggest that increased serum levels of anti-C1q autoantibodies are responsible for apoptosis and may play a pathogenic role in SLE patients, especially in active disease.
    KEYWORDS: anti-C1q; apoptosis; flowcytometry; systemic lupus erythematosus
    Study site: Medical outpatient clinic and medical wards, Hospital Universiti Sains Malaysia (HUSM), Kelantan, Malaysia
    MeSH terms: Adolescent; Adult; Autoantibodies/blood; Autoantibodies/pharmacology*; Autoantibodies/physiology; Cell Line; Cells, Cultured; Cross-Sectional Studies; Enzyme-Linked Immunosorbent Assay; Female; Flow Cytometry; Hospitals, University; Humans; Lupus Erythematosus, Systemic/blood*; Lupus Erythematosus, Systemic/etiology; Lupus Erythematosus, Systemic/immunology*; Lymphocytes/drug effects; Lymphocytes/pathology*; Malaysia; Male; Middle Aged; Outpatient Clinics, Hospital; Propidium; Complement C1q/immunology*; Case-Control Studies; Apoptosis/drug effects*; Annexin A5; Serum/immunology*; Young Adult; In Vitro Techniques
  20. Lau YS, Tian XY, Mustafa MR, Murugan D, Liu J, Zhang Y, et al.
    Br J Pharmacol, 2013 Nov;170(6):1190-8.
    PMID: 23992296 DOI: 10.1111/bph.12350
    Boldine is a potent natural antioxidant present in the leaves and bark of the Chilean boldo tree. Here we assessed the protective effects of boldine on endothelium in a range of models of diabetes, ex vivo and in vitro.
    MeSH terms: Angiotensin II; Animals; Antioxidants/pharmacology*; Aorta, Thoracic/cytology; Aorta, Thoracic/drug effects*; Aorta, Thoracic/physiology; Aporphines/pharmacology*; Cells, Cultured; Diabetes Mellitus, Type 2/metabolism*; Diabetes Mellitus, Type 2/physiopathology; Endothelium, Vascular/drug effects; Endothelium, Vascular/physiology; Male; Mice, Inbred C57BL; Mice, Transgenic; Vasodilation/drug effects; Oxidative Stress; Endothelial Cells/drug effects; Endothelial Cells/metabolism; Receptor, Angiotensin, Type 1/metabolism; Mice; Nitric Oxide Synthase Type III/metabolism; Bone Morphogenetic Protein 4/metabolism*; In Vitro Techniques
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