Browse publications by year: 2016

  1. Veettil SK, Saokaew S, Lim KG, Ching SM, Phisalprapa P, Chaiyakunapruk N
    J Gastrointest Oncol, 2016 Aug;7(4):595-602.
    PMID: 27563450 DOI: 10.21037/jgo.2016.04.02
    BACKGROUND: Colorectal cancer (CRC) is the third most common cancer worldwide and is associated with substantial socioeconomic burden. Despite considerable research, including numerous randomised controlled trials (RCTs) and systematic reviews assessed the effect of various chemopreventive interventions for CRC, there remains uncertainty regarding the comparative effectiveness of these agents. No network meta-analytic study has been published to evaluate the efficacies of these agents for CRC. Therefore, the aim of this study is to summarise the direct and indirect evidence for these interventions to prevent CRC in average-high risk individuals, and to rank these agents for practical consideration.

    METHODS: We will acquire eligible studies through a systematic search of MEDLINE, EMBASE, the Cochrane Central Registry of Controlled Trials, CINAHL plus, IPA and clinicaltrials.gov website. The Cochrane Risk of Bias Tool will be used to assess the quality of included studies. The primary outcomes are the incidence of CRC, the incidence/recurrence of any adenoma or change in polyp burden (number or size). Quantitative synthesis or meta-analysis will be considered. We will also construct a network meta-analysis (NMA) to improve precision of the comparisons among chemo-preventive interventions by combining direct and indirect evidence. The probability of each treatment being the best and/or safest, the number-needed-to-treat [NNT; 95% credible interval (CrIs)], and the number-needed-to-harm (NNH; 95% CrIs) will be calculated to provide measures of treatment efficacy. The GRADE approach will be used to rate the quality of evidence of estimates derived from NMA.

    RESULTS: This protocol has been registered (registration number: CRD42015025849) with the PROSPERO (International Prospective Register of Systematic Reviews). The procedures of this systematic review and NMA will be conducted in accordance with the PRISMA-compliant guideline. The results of this systematic review and NMA will be submitted to a peer-reviewed journal for publication.

    CONCLUSIONS: To the best of our knowledge, this study will be the first NMA to identify the comparative effectiveness of interventions for the prevention of CRC. The results of our study will update evidence for chemoprevention of CRC, identify key areas for future research, and provide a framework for conducting large systematic reviews involving indirect comparisons.

  2. Sahoo S
    Int J Appl Basic Med Res, 2016 Jul-Sep;6(3):166-9.
    PMID: 27563580 DOI: 10.4103/2229-516X.186959
    To know the individual's current level of readiness and to manage self-directed learning (SDL) not only help learners but also the instructors. The objectives of this study were to find SDL readiness among 4(th) year medical student and to analyze the effect of weekly assessment of SDL topics.
    MeSH terms: Education, Medical, Undergraduate; Learning; Students, Medical; Knowledge; Educational Personnel
  3. Al-Majedy YK, Al-Amiery AA, Kadhum AA, Mohamad AB
    Biomed Res Int, 2016;2016:5891703.
    PMID: 27563671 DOI: 10.1155/2016/5891703
    The problem of bacteria resistance to many known agents has inspired scientists and researchers to discover novel efficient antibacterial drugs. Three rapid, clean, and highly efficient methods were developed for one-pot synthesis of 7-(aryl)-10,10-dimethyl-10,11-dihydrochromeno[4,3-b]chromene-6,8(7H,9H)-dione derivatives. Three components are condensed in the synthesis, 4-hydroxycoumarin, 5,5-dimethyl-1,3-cyclohexanedione, and aromatic aldehydes, using tetrabutylammonium bromide (TBAB), diammonium hydrogen phosphate (DAHP), or ferric chloride (FeCl3), respectively. Each method has different reaction mechanisms according to the catalyst. The present methods have advantages, including one-pot synthesis, excellent yields, short reaction times, and easy isolation of product. All catalysts utilized in our study could be reused several times without losing their catalytic efficiency. All synthesized compounds were fully characterized and evaluated for their antibacterial activity.
    MeSH terms: Aldehydes/chemical synthesis; Aldehydes/pharmacology; Anti-Bacterial Agents/chemical synthesis*; Anti-Bacterial Agents/pharmacology; Antioxidants/chemical synthesis; Antioxidants/chemistry; Catalysis; Cyclohexanones/chemical synthesis*; Cyclohexanones/pharmacology; Humans; Staphylococcus aureus/drug effects*; Staphylococcus aureus/pathogenicity; Thiosemicarbazones/chemical synthesis*; Thiosemicarbazones/pharmacology; 4-Hydroxycoumarins/chemical synthesis*; 4-Hydroxycoumarins/pharmacology
  4. Gunggu A, Chang CT, Cheah WL
    J Diabetes Res, 2016;2016:9158943.
    PMID: 27563681 DOI: 10.1155/2016/9158943
    Diabetes mellitus is a public health concern in Malaysia. Treatment of diabetes is costly and can lead to complications if disease is poorly controlled. Diabetes self-management (DSM) is found to be essential for optimal glycemic control. This cross-sectional study was conducted among samples from four randomly selected diabetes clinics in Sarawak, Malaysia. The aim was to determine the predictors for DSM. Face-to-face interview using questionnaire was used to collect data. Four hundred respondents with type 2 diabetes mellitus (T2DM) were recruited. Majority of the respondents were Sarawak Bumiputra (Iban and Bidayuh, 48.6%) and female (68.6%). The mean age was 58.77 years (SD = 11.46) and approximately half of the respondents (50.6%) had T2DM for six years (SD = 4.46). The mean fasting blood glucose (FBG) was 8.06 mmol/L (SD = 2.94), with majority (76.1%) having the level higher than 6.1 mmol/L. Multiple logistic regression tests showed significant linear relationship between DSM and belief in treatment effectiveness (p = 0.001), family support (p = 0.007), and self-efficacy (p = 0.027). Health care personnel must convince patients with T2DM of the effectiveness of the treatment, empower and enhance their self-efficacy, and enlist the family support so as to ensure patients sustain their DSM efforts.
    Study site: diabetes clinics in Kuching and Samarahan Division of Sarawak, Malaysia
    MeSH terms: Aged; Blood Glucose; Borneo; Cross-Sectional Studies; Diabetes Mellitus, Type 2/blood; Diabetes Mellitus, Type 2/drug therapy; Diabetes Mellitus, Type 2/psychology*; Female; Humans; Hypoglycemic Agents/therapeutic use; Insulin/therapeutic use; Health Knowledge, Attitudes, Practice*; Malaysia; Male; Middle Aged; Self Care/psychology*; Blood Glucose Self-Monitoring/psychology*; Treatment Outcome; Self Efficacy*
  5. Carapinha JL
    J Med Econ, 2016 Aug 26.
    PMID: 27564849
    To compare the pharmacoeconomic guidelines in South Africa (SA) with other middle- and high-income countries.
    MeSH terms: Income; Male; Prostate; South Africa; Global Health; Economics, Pharmaceutical; Developed Countries
  6. Fatimah, A.B., Lai, C.Y., Saari, N., Zaman, M.Z.
    MyJurnal
    Raw goat milk is recognized as one kind of nutritious food owed to its originality and
    medicinal values. This study aimed to evaluate the physico-chemical and microbiological
    qualities of locally produced raw goat milk prior any processing steps during storage. Milk
    samples passed organoleptic test and C.O.B. test were mostly (88.89 %) failed in alcohol test.
    AOAC Official method of oven drying method, Kjeldahl method and Soxhlet method were
    performed in physico-chemical analysis where results obtained were partially in lined with
    reported literature due to subjective factors of breeds, geographical areas and feeds. The locally
    produced raw goat milk’s compositions are high in water content and low in fat percentage.
    Initial total plate count, coliform count and proteolytic count tested were 3.44 log cfu/ml, 1.87
    log cfu/ml and 1.97 log cfu/ml, respectively. Storage time showed significant effect on the
    bacterial counts (p>0.05) of milk samples. Shelf-life of milk samples were kept up to 12 hours
    under ambient temperature (3.95 log cfu/ml) had not exceeded the standard limit. The shelflife
    of the milk samples were extended up to 16 days storage under refrigerated temperature
    of 4°C. The microbiological quality of the milk samples showed a significant bacteriological
    growth upon prolonged storage and high initial coliform count indicates possible poor hygienic
    practices at farm level.
    MeSH terms: Agriculture; Alcohols; Animals; Breeding; Goats; Milk; Sensation; Temperature; Water; Biological Processes; Physiological Processes; Bacterial Load
  7. Amosa MK, Jami MS, Alkhatib MF, Majozi T
    Environ Sci Pollut Res Int, 2016 Nov;23(22):22554-22567.
    PMID: 27557958
    This study has applied the concept of the hybrid PAC-UF process in the treatment of the final effluent of the palm oil industry for reuse as feedwater for low-pressure boilers. In a bench-scale set-up, a low-cost empty fruit bunch-based powdered activated carbon (PAC) was employed for upstream adsorption of biotreated palm oil mill effluent (BPOME) with the process conditions: 60 g/L dose of PAC, 68 min of mixing time and 200 rpm of mixing speed, to reduce the feedwater strength, alleviate probable fouling of the membranes and thus improve the process flux (productivity). Three polyethersulfone ultrafiltration membranes of molecular weight cut-off (MWCO) of 1, 5 and 10 kDa were investigated in a cross-flow filtration mode, and under constant transmembrane pressures of 40, 80, and 120 kPa. The permeate qualities of the hybrid processes were evaluated, and it was found that the integrated process with the 1 kDa MWCO UF membrane yielded the best water quality that falls within the US EPA reuse standard for boiler-feed and cooling water. It was also observed that the permeate quality is fit for extended reuse as process water in the cement, petroleum and coal industries. In addition, the hybrid system's operation consumed 37.13 Wh m(-3) of energy at the highest applied pressure of 120 kPa, which is far lesser than the typical energy requirement range (0.8-1.0 kWh m(-3)) for such wastewater reclamation.
    MeSH terms: Adsorption; Charcoal*; Feasibility Studies; Membranes, Artificial; Molecular Weight; Polymers; Pressure; Sulfones; Ultrafiltration; Waste Disposal, Fluid; Water Quality; Waste Water*
  8. Syed Najmuddin SU, Romli MF, Hamid M, Alitheen NB, Nik Abd Rahman NM
    BMC Complement Altern Med, 2016 Aug 24;16(1):311.
    PMID: 27558166 DOI: 10.1186/s12906-016-1290-y
    Annona muricata Linn which comes from Annonaceae family possesses many therapeutic benefits as reported in previous studies and to no surprise, it has been used in many cultures to treat various ailments including headaches, insomnia, and rheumatism to even treating cancer. However, Annona muricata Linn obtained from different cultivation area does not necessarily offer the same therapeutic effects towards breast cancer (in regards to its bioactive compound production). In this study, anti-proliferative and anti-cancer effects of Annona muricata crude extract (AMCE) on breast cancer cell lines were evaluated.
    MeSH terms: Animals; Antineoplastic Agents, Phytogenic/pharmacology*; Breast Neoplasms*; Female; Humans; Nitric Oxide/metabolism; Plant Extracts/pharmacology*; Plant Extracts/chemistry; Apoptosis/drug effects; Plant Leaves/chemistry*; Annona/chemistry*; Cell Line, Tumor; Cell Proliferation/drug effects; Mice
  9. McStea M, McGeechan K, Kamaruzzaman SB, Rajasuriar R, Tan MP
    Postgrad Med, 2016 Nov;128(8):797-804.
    PMID: 27558757 DOI: 10.1080/00325481.2016.1229103
    Metabolic Syndrome (METs) definitions vary and diagnosis takes into account consumption of medications commonly prescribed for conditions defining METs. This paper evaluates the potential differences in population characteristics using two different methods of defining METs, with and without the adjustment of the effects of pharmacotherapy on biochemical and blood pressure (BP) measurements Methods: This was a cross-sectional study utilizing the Malaysian Elders Longitudinal Research (MELoR) cohort comprising urban community-dwellers aged ≥55 years. Participants were interviewed using a structured questionnaire during home visits where medications were reviewed. Health impacts assessed included heart disease, stroke, body mass index (BMI), peptic ulcers, arthritis, and number of medications and comorbidities. Risk factors and health impacts associated with METs were determined by Poisson multivariate regression models using a binary and count dependent variables.
    MeSH terms: Age Factors; Aged; Aged, 80 and over; Hypolipidemic Agents/administration & dosage*; Cardiovascular Agents/administration & dosage*; Cross-Sectional Studies; Female; Humans; Hypoglycemic Agents/administration & dosage*; Longitudinal Studies; Malaysia/epidemiology; Male; Middle Aged; Risk Factors; Sex Factors; Socioeconomic Factors; Comorbidity; Body Mass Index; Prevalence; Polypharmacy*; Metabolic Syndrome X/epidemiology*
  10. Gill MR, Harun SN, Halder S, Boghozian RA, Ramadan K, Ahmad H, et al.
    Sci Rep, 2016 08 25;6:31973.
    PMID: 27558808 DOI: 10.1038/srep31973
    Ruthenium(II) polypyridyl complexes can intercalate DNA with high affinity and prevent cell proliferation; however, the direct impact of ruthenium-based intercalation on cellular DNA replication remains unknown. Here we show the multi-intercalator [Ru(dppz)2(PIP)](2+) (dppz = dipyridophenazine, PIP = 2-(phenyl)imidazo[4,5-f][1,10]phenanthroline) immediately stalls replication fork progression in HeLa human cervical cancer cells. In response to this replication blockade, the DNA damage response (DDR) cell signalling network is activated, with checkpoint kinase 1 (Chk1) activation indicating prolonged replication-associated DNA damage, and cell proliferation is inhibited by G1-S cell-cycle arrest. Co-incubation with a Chk1 inhibitor achieves synergistic apoptosis in cancer cells, with a significant increase in phospho(Ser139) histone H2AX (γ-H2AX) levels and foci indicating increased conversion of stalled replication forks to double-strand breaks (DSBs). Normal human epithelial cells remain unaffected by this concurrent treatment. Furthermore, pre-treatment of HeLa cells with [Ru(dppz)2(PIP)](2+) before external beam ionising radiation results in a supra-additive decrease in cell survival accompanied by increased γ-H2AX expression, indicating the compound functions as a radiosensitizer. Together, these results indicate ruthenium-based intercalation can block replication fork progression and demonstrate how these DNA-binding agents may be combined with DDR inhibitors or ionising radiation to achieve more efficient cancer cell killing.
    MeSH terms: Animals; Cattle; Cell Line; DNA/metabolism; DNA Damage/drug effects; DNA Damage/radiation effects; DNA Repair/drug effects; DNA Replication/drug effects*; HeLa Cells; Histones/metabolism; Humans; Intercalating Agents/metabolism; Intercalating Agents/toxicity; Intercalating Agents/chemistry*; Phosphorylation/drug effects; Quinolines/toxicity; Quinuclidines/toxicity; Radiation-Sensitizing Agents/metabolism; Radiation-Sensitizing Agents/toxicity; Radiation-Sensitizing Agents/chemistry*; Radiation, Ionizing; Ruthenium/chemistry*; Gene Expression/drug effects; Gene Expression/radiation effects; Apoptosis/drug effects; Apoptosis/radiation effects; BRCA1 Protein/genetics; BRCA1 Protein/metabolism; Cell Proliferation/drug effects; Coordination Complexes/metabolism; Coordination Complexes/toxicity; Coordination Complexes/chemistry*; G1 Phase Cell Cycle Checkpoints/drug effects; Checkpoint Kinase 1/antagonists & inhibitors; Checkpoint Kinase 1/metabolism*
  11. Yap FB, Kiung ST, Yap JB
    Indian Dermatol Online J, 2016 Jul-Aug;7(4):255-8.
    PMID: 27559497 DOI: 10.4103/2229-5178.185495
    There is a paucity of data on quality of life issues in patients with leprosy suffering from erythema nodosum leprosum (ENL). Thus, we aim to study the effect of ENL on quality of life.
    MeSH terms: Erythema Nodosum; Humans; Leprosy; Quality of Life; Leprosy, Lepromatous
  12. Huang CK, Wang HH, Nor Hanipah Z
    J Laparoendosc Adv Surg Tech A, 2016 Nov;26(11):921-924.
    PMID: 27560500
    Peritoneal dialysate leakage is a well-known complication of continuous ambulatory peritoneal dialysis (CAPD). In late leakage, it is usually managed conservatively and subsequently converted to hemodialysis. We hereby report a case of peritoneal dialysate leakage secondary to necrotic peritoneum, which was managed by laparoscopic excision of the affected peritoneum. Regeneration of new peritoneum was documented and the patient could resume CAPD successfully.
    MeSH terms: Humans; Male; Middle Aged; Necrosis; Peritoneal Dialysis, Continuous Ambulatory/adverse effects*; Peritoneal Diseases/etiology; Peritoneal Diseases/surgery*; Laparoscopy/methods*; Peritoneum/pathology; Peritoneum/surgery*; Phytic Acid; Tomography, X-Ray Computed; Dialysis Solutions; Organotechnetium Compounds; Radiopharmaceuticals
  13. Seman-Kamarulzaman AF, Mohamed-Hussein ZA, Ng CL, Hassan M
    PLoS One, 2016;11(8):e0161707.
    PMID: 27560927 DOI: 10.1371/journal.pone.0161707
    Juvenile Hormone III is of great concern due to negative effects on major developmental and reproductive maturation in insect pests. Thus, the elucidation of enzymes involved JH III biosynthetic pathway has become increasing important in recent years. One of the enzymes in the JH III biosynthetic pathway that remains to be isolated and characterized is farnesal dehydrogenase, an enzyme responsible to catalyze the oxidation of farnesal into farnesoic acid. A novel NAD+-farnesal dehydrogenase of Polygonum minus was purified (315-fold) to apparent homogeneity in five chromatographic steps. The purification procedures included Gigacap S-Toyopearl 650M, Gigacap Q-Toyopearl 650M, and AF-Blue Toyopearl 650ML, followed by TSK Gel G3000SW chromatographies. The enzyme, with isoelectric point of 6.6 is a monomeric enzyme with a molecular mass of 70 kDa. The enzyme was relatively active at 40°C, but was rapidly inactivated above 45°C. The optimal temperature and pH of the enzyme were found to be 35°C and 9.5, respectively. The enzyme activity was inhibited by sulfhydryl agent, chelating agent, and metal ion. The enzyme was highly specific for farnesal and NAD+. Other terpene aldehydes such as trans- cinnamaldehyde, citral and α- methyl cinnamaldehyde were also oxidized but in lower activity. The Km values for farnesal, citral, trans- cinnamaldehyde, α- methyl cinnamaldehyde and NAD+ were 0.13, 0.69, 0.86, 1.28 and 0.31 mM, respectively. The putative P. minus farnesal dehydrogenase that's highly specific towards farnesal but not to aliphatic aldehydes substrates suggested that the enzyme is significantly different from other aldehyde dehydrogenases that have been reported. The MALDI-TOF/TOF-MS/MS spectrometry further identified two peptides that share similarity to those of previously reported aldehyde dehydrogenases. In conclusion, the P. minus farnesal dehydrogenase may represent a novel plant farnesal dehydrogenase that exhibits distinctive substrate specificity towards farnesal. Thus, it was suggested that this novel enzyme may be functioning specifically to oxidize farnesal in the later steps of JH III pathway. This report provides a basic understanding for recombinant production of this particular enzyme. Other strategies such as adding His-tag to the protein makes easy the purification of the protein which is completely different to the native protein. Complete sequence, structure and functional analysis of the enzyme will be important for developing insect-resistant crop plants by deployment of transgenic plant.
    MeSH terms: Acrolein/analogs & derivatives; Acrolein/chemistry; Aldehyde Dehydrogenase/metabolism; Aldehyde Dehydrogenase/chemistry*; Aldehyde Oxidoreductases/metabolism; Aldehyde Oxidoreductases/chemistry*; Farnesol/analogs & derivatives; Farnesol/chemistry; Metals/chemistry; Plant Proteins/metabolism; Plant Proteins/chemistry*; Sesquiterpenes/metabolism*; Substrate Specificity; Plant Leaves/enzymology; Plant Leaves/metabolism; Polygonum/enzymology*; Polygonum/metabolism; Monoterpenes/chemistry
  14. Tanuma J, Jiamsakul A, Makane A, Avihingsanon A, Ng OT, Kiertiburanakul S, et al.
    PLoS One, 2016;11(8):e0161562.
    PMID: 27560968 DOI: 10.1371/journal.pone.0161562
    BACKGROUND: In resource-limited settings, routine monitoring of renal function during antiretroviral therapy (ART) has not been recommended. However, concerns for tenofovir disoproxil fumarate (TDF)-related nephrotoxicity persist with increased use.

    METHODS: We investigated serum creatinine (S-Cr) monitoring rates before and during ART and the incidence and prevalence of renal dysfunction after starting TDF by using data from a regional cohort of HIV-infected individuals in the Asia-Pacific. Time to renal dysfunction was defined as time from TDF initiation to the decline in estimated glomerular filtration rate (eGFR) to <60 ml/min/1.73m2 with >30% reduction from baseline using the Chronic Kidney Disease Epidemiology Collaboration (CKD-EPI) equation or the decision to stop TDF for reported TDF-nephrotoxicity. Predictors of S-Cr monitoring rates were assessed by Poisson regression and risk factors for developing renal dysfunction were assessed by Cox regression.

    RESULTS: Among 2,425 patients who received TDF, S-Cr monitoring rates increased from 1.01 to 1.84 per person per year after starting TDF (incidence rate ratio 1.68, 95%CI 1.62-1.74, p <0.001). Renal dysfunction on TDF occurred in 103 patients over 5,368 person-years of TDF use (4.2%; incidence 1.75 per 100 person-years). Risk factors for developing renal dysfunction included older age (>50 vs. ≤30, hazard ratio [HR] 5.39, 95%CI 2.52-11.50, p <0.001; and using PI-based regimen (HR 1.93, 95%CI 1.22-3.07, p = 0.005). Having an eGFR prior to TDF (pre-TDF eGFR) of ≥60 ml/min/1.73m2 showed a protective effect (HR 0.38, 95%CI, 0.17-0.85, p = 0.018).

    CONCLUSIONS: Renal dysfunction on commencing TDF use was not common, however, older age, lower baseline eGFR and PI-based ART were associated with higher risk of renal dysfunction during TDF use in adult HIV-infected individuals in the Asia-Pacific region.

    MeSH terms: Tenofovir/adverse effects*; Tenofovir/therapeutic use; Adult; Creatinine/blood; Female; Glomerular Filtration Rate; Humans; Kidney/drug effects*; Kidney/physiopathology*; Kidney Diseases/chemically induced*; Male; Middle Aged; Prospective Studies; Risk Factors; HIV Infections/drug therapy*; Poisson Distribution; Proportional Hazards Models; Databases, Factual; Anti-HIV Agents/adverse effects*; Anti-HIV Agents/therapeutic use
  15. Norizzah, A.R., Junaida, A.R., Maryam ‘Afifah, A.L.
    MyJurnal
    The aim of this study was to determine the effects of xanthan gum and carrageenan on the
    oil uptake and acceptability of banana (Musa acuminate) fritters during repeated deep fat
    frying. Banana namely ‘Pisang Awak’ at maturity stage 6 were peeled, cut and dipped into 3
    batter formulations containing 1% carrageenan, 1% xanthan gum and a control. The bananas
    were deep fried at 170±5°C for 3 minutes in 2.5L cooking oil without oil replenishment for
    3 consecutive days. The moisture, oil content, texture, colour and acceptability of the banana
    fritters were evaluated at first and every 10th frying cycles. Results indicated that the oil and
    moisture content of fried bananas were dependent on frying cycles. The oil content increased
    while the moisture decreased with increased in frying cycles. There was significant reduction
    (p0.05) in terms of overall acceptance between
    treated and untreated. Hence, 1% xanthan gum was effective in reducing oil absorption of
    banana fritters without affecting the overall sensory acceptability
    MeSH terms: Carrageenan; Color; Cooking; Excipients; Polysaccharides, Bacterial; Musa
  16. Conell J, Bauer R, Glenn T, Alda M, Ardau R, Baune BT, et al.
    Int J Bipolar Disord, 2016 Dec;4(1):17.
    PMID: 27552813 DOI: 10.1186/s40345-016-0058-0
    BACKGROUND: Information seeking is an important coping mechanism for dealing with chronic illness. Despite a growing number of mental health websites, there is little understanding of how patients with bipolar disorder use the Internet to seek information.

    METHODS: A 39 question, paper-based, anonymous survey, translated into 12 languages, was completed by 1222 patients in 17 countries as a convenience sample between March 2014 and January 2016. All patients had a diagnosis of bipolar disorder from a psychiatrist. Data were analyzed using descriptive statistics and generalized estimating equations to account for correlated data.

    RESULTS: 976 (81 % of 1212 valid responses) of the patients used the Internet, and of these 750 (77 %) looked for information on bipolar disorder. When looking online for information, 89 % used a computer rather than a smartphone, and 79 % started with a general search engine. The primary reasons for searching were drug side effects (51 %), to learn anonymously (43 %), and for help coping (39 %). About 1/3 rated their search skills as expert, and 2/3 as basic or intermediate. 59 % preferred a website on mental illness and 33 % preferred Wikipedia. Only 20 % read or participated in online support groups. Most patients (62 %) searched a couple times a year. Online information seeking helped about 2/3 to cope (41 % of the entire sample). About 2/3 did not discuss Internet findings with their doctor.

    CONCLUSION: Online information seeking helps many patients to cope although alternative information sources remain important. Most patients do not discuss Internet findings with their doctor, and concern remains about the quality of online information especially related to prescription drugs. Patients may not rate search skills accurately, and may not understand limitations of online privacy. More patient education about online information searching is needed and physicians should recommend a few high quality websites.

  17. Hussein FE, Liew AK, Ramlee RA, Abdullah D, Chong BS
    J Endod, 2016 Oct;42(10):1441-5.
    PMID: 27552839 DOI: 10.1016/j.joen.2016.07.009
    INTRODUCTION: Ignoring the cluster effect is a common statistical oversight that is also observed in endodontic research. The aim of this study was to explore the use of multilevel modeling in investigating the effect of tooth-level and patient-level factors on apical periodontitis (AP).

    METHODS: A random sample of digital panoramic radiographs from the database of a dental hospital was evaluated. Two calibrated examiners (κ ≥ 0.89) assessed the technical quality of the root fillings and the radiographic periapical health status by using the periapical index. Descriptive statistical analysis was carried out, followed by multilevel modeling by using tooth-level and patient-level predictors. Model fit information was obtained, and the findings of the best-fit model were reported.

    RESULTS: A total of 6409 teeth were included in the analysis. The predicted probability of a tooth having AP was 0.42%. There was a statistically significant variability between patients (P 

    MeSH terms: Adolescent; Adult; Age Factors; Aged; Female; Humans; Male; Middle Aged; Periapical Periodontitis/diagnosis*; Periapical Periodontitis/therapy; Radiography, Panoramic; Retrospective Studies; Multilevel Analysis; Young Adult
  18. Khan MS, Majid AM, Iqbal MA, Majid AS, Al-Mansoub M, Haque RS
    Eur J Pharm Sci, 2016 Oct 10;93:304-18.
    PMID: 27552907 DOI: 10.1016/j.ejps.2016.08.032
    Glioblastoma multiforme is a highly malignant, heterogenic, and drug resistant tumor. The blood-brain barrier (BBB), systemic cytotoxicity, and limited specificity are the main obstacles in designing brain tumor drugs. In this study a computational approach was used to design brain tumor drugs that could downregulate VEGF and IL17A in glioblastoma multiforme type four. Computational screening tools were used to evaluate potential candidates for antiangiogenic activity, target binding, BBB permeability, and ADME physicochemical properties. Additionally, in vitro cytotoxicity, migration, invasion, tube formation, apoptosis, ROS and ELISA assays were conducted for molecule 6 that was deemed most likely to succeed. The efflux ratio of membrane permeability and calculated docking scores of permeability to glycoproteins (P-gps) were used to determine the BBB permeability of the molecules. The results showed BBB permeation for molecule 6, with the predicted efficiency of 0.55kcal/mol and binding affinity of -37kj/mol corresponding to an experimental efflux ratio of 0.625 and predicted -15kj/mol of binding affinity for P-gps. Molecule 6 significantly affected the angiogenesis pathways by 2-fold downregulation of IL17A and VEGF through inactivation of active sites of HSP90 (predicted binding: -37kj/mol, predicted efficiency: 0.55kcal/mol) and p23 (predicted binding: 12kj/mol, predicted efficiency: 0.17kcal/mol) chaperon proteins. Additionally, molecule 6 activated the 17.38% relative fold of ROS level at 18.3μg/mL and upregulated the caspase which lead the potential synergistic apoptosis through the antiangiogenic activity of molecule 6 and thereby the highly efficacious anticancer upshot. The results indicate that the binding of the molecules to the therapeutic target is not essential to produce a lethal effect on cancer cells of the brain and that antiangiogenic efficiency is much more important.
    MeSH terms: Animals; Aorta/drug effects; Aorta/physiology; Blood-Brain Barrier/metabolism; Brain Neoplasms/metabolism*; Cell Line; Cell Movement/drug effects; Cell Survival/drug effects; Computer Simulation; Glioblastoma/metabolism*; Humans; Imidazoles/pharmacology*; Imidazoles/chemistry; Male; Models, Biological; Rats, Sprague-Dawley; Reactive Oxygen Species/metabolism; Caspases/metabolism; Interleukin-17/metabolism*; Angiogenesis Inhibitors/pharmacology*; Angiogenesis Inhibitors/chemistry; Quantitative Structure-Activity Relationship; Vascular Endothelial Growth Factor A/metabolism*; Cell Proliferation/drug effects; Molecular Docking Simulation; In Vitro Techniques
  19. Nasreen HE, Alam MA, Edhborg M
    PMID: 27553260 DOI: 10.1111/jcap.12150
    Few studies have examined the adolescents' depression in low-income countries and no research has yet been carried out in Bangladesh. This study estimated the prevalence of depressive symptoms and explored the associated factors and help seeking behavior among adolescents in Bangladesh.
  20. Mohd Zaid NA, Idris NH
    Sci Rep, 2016 08 24;6:32082.
    PMID: 27553290 DOI: 10.1038/srep32082
    In this work, Ni nanoparticles were directly decorated on graphene (G) nanosheets via mechanical ball milling. Based on transmission electron microscopy observations, the Ni nanoparticles were well dispersed and attached to the G nanosheet without any agglomerations. Electrochemical results showed that the capacitance of a G/Ni nanocomposite was 275 F g(-1) at a current density of 2 A g(-1), which is higher than the capacitance of bare G (145 F g(-1)) and bare Ni (3 F g(-1)). The G/Ni electrode also showed superior performance at a high current density, exhibiting a capacitance of 190 F g(-1) at a current density of 5 A g(-1) and a capacitance of 144 F g(-1) at a current density of 10 A g(-1). The equivalent series resistance for G/Ni nanocomposites also decreased. The enhanced performance of this hybrid supercapacitor is best described by the synergistic effect, i.e. dual charge-storage mechanism, which is demonstrated by electrical double layer and pseudocapacitance materials. Moreover, a high specific surface area and electrical conductivity of the materials enhanced the capacitance. These results indicate that the G/Ni nanocomposite is a potential supercapacitor.
    MeSH terms: Electric Conductivity; Electrodes; Graphite; Electric Capacitance; Microscopy, Electron, Transmission; Nanoparticles; Nanocomposites
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