Browse publications by year: 2016

  1. Yeh CC, Muduli S, Peng IC, Lu YT, Ling QD, Alarfaj AA, et al.
    Data Brief, 2016 Mar;6:603-8.
    PMID: 26909373 DOI: 10.1016/j.dib.2015.12.056
    This data article contains two figures and one table supporting the research article entitled: "Continuous harvest of stem cells via partial detachment from thermoresponsive nanobrush surface" [1]. The table shows coating conditions of three copolymers, poly(styrene-co-acrylic acid) grafted with oligovitronectin, poly(styrene-co-N-isopropylacrylamide) and poly(styrene-co-polyethylene glycol methacrylate) to prepare thermoresponsive surface. XPS spectra show the nitrogen peak of the polystyrene surface coated with poly(styrene-co-acrylic acid) grafted with oligovitronectin. The surface coating density analyzed from sorption of poly(styrene-co-acrylic acid) grafted with oligovitronectin by UV-vis spectroscopy is also presented.
    MeSH terms: Acrylamides; Acrylic Resins; Longitudinal Studies; Methacrylates; Nitrogen; Polyethylene Glycols; Polymethacrylic Acids; Polystyrenes; Spectrum Analysis; Stem Cells
  2. Abdurabu WA, Ramli AT, Saleh MA, Heryansyah A, Alnhary A, Fadhl S
    J Radiol Prot, 2016 Mar;36(1):163-77.
    PMID: 26909670 DOI: 10.1088/0952-4746/36/1/163
    This study aims to evaluate natural radiation and radioactivity in the rock and to assess the corresponding health risk in a region of elevated background radiation in Juban District, Ad Dali' Governorate, Yemen. The mean external gamma dose rate was 374 nGy h(-1) which is approximately six times the world average. The measured results were used to compute annual effective dose equivalent, collective effective dose and excess lifetime cancer risk, which are 2.298 mSv, 61.95 man Sv y(-1) and 8.043  ×  10(-3), respectively. Rocks samples from different geological formations were analyzed for quantitative determination of (226)Ra, (232)Th and (40)K. The specific activity of the rocks samples ranges from 7  ±  1 Bq Kg(-1) to 12 513  ±  329 Bq Kg(-1) for (232)Th, from 6  ±  1 Bq kg(-1) to 3089  ±  74 Bq kg(-1) for (226)Ra and 702  ±  69 Bq kg(-1) to 2954  ±  285 Bq kg(-1) for (40)K. (232)Th is the main contributor to gamma dose rate from the rock samples. Indicators of radiological health impact, radium equivalent activity and external hazard index are 3738 Bq kg(-1) and 10.10, respectively. The mean external hazard index was ten times unity in the studied locations in Juban District, which is higher than the recommended value.
  3. Chan CYW, Chiu CK, Kwan MK
    Spine (Phila Pa 1976), 2016 Aug 15;41(16):E973-E980.
    PMID: 26909833 DOI: 10.1097/BRS.0000000000001516
    STUDY DESIGN: A prospective study.

    OBJECTIVE: The aim of this study was to analyze the proximal thoracic (PT) flexibility and its compensatory ability above the "potential UIV."

    SUMMARY OF BACKGROUND DATA: Shoulder and neck imbalance can be caused by overcorrection of the main thoracic (MT) curve due to inability of PT segment to compensate.

    METHODS: Cervical supine side bending (CSB) radiographs of 100 Lenke 1 and 2 patients were studied. We further stratified Lenke 1 curves into Lenke 1-ve: PT side bending (PTSB) 80.0% of cases of the PT segment were unable to compensate at T3-T6. In Lenke 1+ve curves, 78.4% were unable to compensate at T6, followed by T5 (75.7%), T4 (73.0%), T3 (59.5%), T2 (27.0%), and T1 (21.6%). In Lenke 1-ve curves, 36.4% of cases were unable to compensate at T6, followed by T5 (45.5%), T4 (45.5%), T3 (30.3%), T2 (21.2%), and T1 (15.2%). A significant difference between Lenke 1-ve and Lenke 1+ve was observed from T3 to T6. The difference between Lenke 1+ve and Lenke 2 curves was significant only at T2.

    CONCLUSION: The compensation ability and the flexibility of the PT segments of Lenke 1-ve and Lenke 1+ve curves were different. Lenke 1+ve curves demonstrated similar characteristics to Lenke 2 curves.

    LEVEL OF EVIDENCE: 3.

    MeSH terms: Adolescent; Child; Female; Humans; Kyphosis/physiopathology; Male; Posture/physiology; Preoperative Care; Prospective Studies; Radiography; Scoliosis/physiopathology; Spinal Fusion/methods; Range of Motion, Articular/physiology*; Pliability/physiology*; Young Adult
  4. Saminathan M, Sieo CC, Gan HM, Ravi S, Venkatachalam K, Abdullah N, et al.
    J Sci Food Agric, 2016 Oct;96(13):4565-74.
    PMID: 26910767 DOI: 10.1002/jsfa.7674
    BACKGROUND: Condensed tannin (CT) fractions of different molecular weights (MWs) may affect rumen microbial metabolism by altering bacterial diversity. In this study the effects of unfractionated CTs (F0) and five CT fractions (F1-F5) of different MWs (F1, 1265.8 Da; F2, 1028.6 Da; F3, 652.2 Da; F4, 562.2 Da; F5, 469.6 Da) from Leucaena leucocephala hybrid-Rendang (LLR) on the structure and diversity of the rumen bacterial community were investigated in vitro.

    RESULTS: Real-time polymerase chain reaction assay showed that the total bacterial population was not significantly (P > 0.05) different among the dietary treatments. Inclusion of higher-MW CT fractions F1 and F2 significantly (P 

    MeSH terms: Clostridiales/classification; Clostridiales/growth & development; Clostridiales/isolation & purification; Clostridiales/metabolism; Gastrointestinal Microbiome; Animals; Cattle; Crosses, Genetic; Diet/veterinary*; Digestion; Gastrointestinal Contents/microbiology*; Fabaceae/chemistry*; Male; Molecular Weight; Rumen/microbiology*; Species Specificity; Plant Leaves/chemistry; Plant Shoots/chemistry; Prevotella/classification; Prevotella/growth & development; Prevotella/isolation & purification; Prevotella/metabolism; Proanthocyanidins/administration & dosage*; Proanthocyanidins/isolation & purification; Proanthocyanidins/metabolism; Proanthocyanidins/chemistry; Fibrobacter/classification; Fibrobacter/growth & development*; Fibrobacter/isolation & purification; Fibrobacter/metabolism; Ruminococcus/classification; Ruminococcus/growth & development*; Ruminococcus/isolation & purification; Ruminococcus/metabolism; Microbial Viability; Molecular Typing/veterinary
  5. Poon WC, Herath G, Sarker A, Masuda T, Kada R
    Appl Ergon, 2016 Feb 21.
    PMID: 26911247 DOI: 10.1016/j.apergo.2016.02.009
    Human activities, such as industrial, agricultural, and domestic pursuits, discharge effluents into riverine ecological systems that contains aquatic resources, such as fish, which are also used by humans. We conducted case studies in Malaysia to investigate the impacts of these human activities on water and fish resources, as well as on human well-being from an ergonomics perspective. This research shows that a green ergonomics approach can provide us with useful insights into sustainable relationships between humans and ecology in facilitating human well-being in consideration of the overall performance of the social-ecological system. Heavy metal concentrations contained in the effluents pollute river water and contaminate fish, eventually creating significant health risks and economic costs for residents, including the polluters. The study suggests a number of policy interventions to change human behavior and achieve greater collaboration between various levels of government, academia, civil society, and businesses to help establish sustainable relationships between humans and ecology in Malaysia.
    MeSH terms: Animals; Ecology; Fishes; Government; Humans; Human Activities; Malaysia; Water; Ecosystem; Metals, Heavy; Rivers
  6. Rajandram R, Ong TA, Razack AH, MacIver B, Zeidel M, Yu W
    Am J Physiol Renal Physiol, 2016 05 01;310(9):F885-94.
    PMID: 26911853 DOI: 10.1152/ajprenal.00483.2015
    Ketamine is a popular choice for young drug abusers. Ketamine abuse causes lower urinary tract symptoms, with the underlying pathophysiology poorly understood. Disruption of urothelial barrier function has been hypothesized to be a major mechanism for ketamine cystitis, yet the direct evidence of impaired urothelial barrier function is still lacking. To address this question, 8-wk-old female C57BL/6J mice were injected intraperitoneally with 30 mg·kg(-1)·day(-1) ketamine for 12 wk to induce ketamine cystitis. A spontaneous voiding spot assay showed that ketamine-treated mice had increased primary voiding spot numbers and smaller primary voiding spot sizes than control mice (P < 0.05), indicating a contracted bladder and bladder overactivity. Consistently, significantly increased voiding frequency was observed in ketamine-treated mice on cystometrograms. These functional experiments indicate that ketamine induces voiding dysfunction in mice. Surprisingly, urothelial permeability in ketamine-treated mice was not changed when measured using an Ussing chamber system with isotopic urea and water. Mouse urothelial structure was also not altered, and intact umbrella cell structure was observed by both transmission and scanning electron microscopy. Furthermore, immunostaining and confocal microscopy confirmed the presence of a well-defined distribution of zonula occuldens-1 in tight junctions and uroplakin in umbrella cells. In conclusion, these data indicate that ketamine injection induces voiding dysfunction in mice but does not necessarily disrupt mouse bladder barrier function. Disruption of urothelial barrier function may not be the major mechanism in ketamine cystitis.
    MeSH terms: Anesthetics, Dissociative; Animals; Urinary Bladder/physiopathology; Cystitis/chemically induced*; Cystitis/pathology*; Female; Ketamine; Mice, Inbred C57BL; Permeability; Urothelium/pathology*; Urothelium/ultrastructure; Mice; Urinary Bladder, Overactive/chemically induced; Urinary Bladder, Overactive/physiopathology; Tight Junction Proteins/metabolism; Zonula Occludens-1 Protein/metabolism
  7. Zakaria ZA, Balan T, Azemi AK, Omar MH, Mohtarrudin N, Ahmad Z, et al.
    BMC Complement Altern Med, 2016 Feb 24;16:78.
    PMID: 26912079 DOI: 10.1186/s12906-016-1041-0
    BACKGROUND: Muntingia calabura L. (family Muntingiaceae), commonly known as Jamaican cherry or kerukup siam in Malaysia, is used traditionally to treat various ailments. The aim of this study is to elucidate the possible underlying gastroprotective mechanisms of ethyl acetate fraction (EAF) of Muntingia calabura methanolic leaves extract (MEMC).

    METHODS: MEMC and its fractions were subjected to HPLC analysis to identify and quantify the presence of its phyto-constituents. The mechanism of gastroptotection of EAF was further investigated using pylorus ligation-induced gastric lesion rat model (100, 250, and 500 mg/kg). Macroscopic analysis of the stomach, evaluation of gastric content parameters such as volume, pH, free and total acidity, protein estimation, and quantification of mucus were carried out. The participation of nitric oxide (NO) and sulfhydryl (SH) compounds was evaluated and the superoxide dismutase (SOD), gluthathione (GSH), catalase (CAT), malondialdehyde (MDA), prostaglandin E2 (PGE2) and NO level in the ethanol induced stomach tissue homogenate was determined.

    RESULTS: HPLC analysis confirmed the presence of quercetin and gallic acid in EAF. In pylorus-ligation model, EAF significantly (p <0.001) prevent gastric lesion formation. Volume of gastric content and total protein content reduced significantly (p 

    MeSH terms: Animals; Anti-Ulcer Agents/analysis; Anti-Ulcer Agents/pharmacology; Anti-Ulcer Agents/therapeutic use; Antioxidants/analysis; Antioxidants/pharmacology*; Antioxidants/therapeutic use; Disease Models, Animal; Gallic Acid/analysis; Gallic Acid/pharmacology*; Gallic Acid/therapeutic use; Gastric Mucosa/drug effects; Gastric Mucosa/metabolism; Male; Malondialdehyde/blood; Phytotherapy; Mucus/metabolism; Nitric Oxide/metabolism; Plant Extracts/pharmacology*; Plant Extracts/therapeutic use; Quercetin/analysis; Quercetin/pharmacology*; Quercetin/therapeutic use; Stomach/drug effects*; Stomach/pathology; Dinoprostone/metabolism; Rats, Sprague-Dawley; Oxidative Stress/drug effects; Plant Leaves/chemistry; Angiosperms/chemistry*
  8. Yusof AM, Abd Ghafar N, Kamarudin TA, Hui CK, Yusof YA
    PMID: 26912349 DOI: 10.1186/s12906-016-1055-7
    This study aimed to evaluate the effects of Gelam honey on corneal keratocytes proliferative capacity and phenotypic characterization via MTT assay, gene expression and immunocytochemistry.
  9. Pua U, Tang AA, Ooi YW, Ho HH, Ong PJ
    Vasc Endovascular Surg, 2016 Jan;50(1):63-7.
    PMID: 26912530 DOI: 10.1177/1538574416629564
    Insufficient renal artery length for renal sympathetic denervation (RDN) is defined as having a main renal artery shorter than 20 mm in length. Such an anatomy is considered a contraindication for most of the currently available endovascular RDN devices. The concern stems from the need to distribute the ablation points to effect circumferential ablation causing sympathetic denervation, without the risk of injuring the renal artery. We postulate that if the requisite ablation points could be distributed between large caliber renal branches and the short main renal artery, RDN is likely feasible and safe. We demonstrate this with 2 cases using 2 different RDN devices and detail the technical feasibility.
  10. Taguchi T, Dillon J, Yakub MA
    Heart Surg Forum, 2016;19(1):E033-5.
    PMID: 26913683 DOI: 10.1532/hsf.1359
    A 55-year-old man developed severe mitral regurgitation with persistent fungal infective endocarditis 8 months after coronary artery bypass grafting with a left internal mammary artery and 2 saphenous veins, as well as mitral valve repair with a prosthetic ring. Echocardiography demonstrated severe mitral regurgitation and a valvular vegetation. Computed tomography coronary arteriography indicated that all grafts were patent and located intimately close to the sternum. Median resternotomy was not attempted due to the risk of injury to the bypass grafts, and therefore, a right anterolateral thoracotomy approach was utilized. Mitral valve replacement was performed with the patient under deep hypothermia and ventricular fibrillation without aortic cross-clamping. The patient`s postoperative course was uneventful. Thus, right anterolateral thoracotomy may be a superior approach to mitral valve surgery in patients who have undergone prior coronary artery bypass grafting.
  11. Taha NA, See YL
    Int J Pharm Pract, 2016 Oct;24(5):326-32.
    PMID: 26914019 DOI: 10.1111/ijpp.12256
    OBJECTIVES: The risk for travel-related illnesses has increased with significant growth in international travel, but very few travellers seek travel advice. Community pharmacists can play a vital role in the provision of travel medicine advice due to their accessibility. This study aimed to assess travel medicine knowledge, attitudes and practices (KAP) among community pharmacists in Kuala Lumpur, Malaysia.

    METHODS: A self-administered KAP questionnaire was distributed to a convenience sample of pharmacists in Kuala Lumpur identified from the list of licensed community pharmacists in Malaysia year 2014.

    KEY FINDINGS: Questionnaires were returned by 111 pharmacists of 143 distributed (response rate, 78%). Most of the respondents (82%) were not trained in travel medicine. Overall, mean knowledge score was 4.4 ( ± 1.7), indicating a moderate level of knowledge on a variety of travel-related health issues. Community pharmacists who graduated from foreign universities possessed significantly higher knowledge scores than did those who graduated locally (P < 0.05). The majority had a positive attitude towards travel medicine. A vast majority provided travel medicine advice mainly to adults who travel as tourists, and the primary travel advice given was on traveller's diarrhoea.

    CONCLUSION: There are gaps in the knowledge and practice of travel medicine among Malaysian pharmacists. Positive attitudes of pharmacists towards travel medicine and appropriate interventions, such as incorporation of travel medicine in local pharmacy curricula, continuous pharmacy education or certified training may improve the quality of travel advice given and allow pharmacists to be recognised as a credible source of information on travel medicine.

    MeSH terms: Adult; Community Pharmacy Services*; Cross-Sectional Studies; Female; Humans; Health Knowledge, Attitudes, Practice*; Malaysia; Male; Middle Aged; Pharmacists/psychology*; Surveys and Questionnaires; Professional Role*; Young Adult; Travel Medicine/education*
  12. Krishnan A, Brown SE, Ghani MA, Khan F, Kamarulzaman A, Altice FL
    Subst Abus, 2016 Oct-Dec;37(4):542-549.
    PMID: 26914230
    BACKGROUND: Drug use in Malaysia remains a significant public health and social problem despite implementation of harsh punitive drug policies such as forcibly placing suspected drug users into compulsory drug detention centers (CDDCs). Following criticism over human right violations in CDDCs, Malaysia has begun to transition towards voluntary drug treatment centers known as Cure & Care (C&C) centers. To best serve the needs of regional C&C centers, data on drug use are essential among patients accessing treatment. Using a mixed-methods approach, the authors examined pretreatment drug use characteristics and experiences with addiction treatment among C&C patients in Kelantan-a religiously conservative state in northeast Malaysia with high prevalence of drug use but where limited data are available on drug use patterns.
    METHODS: A mixed-methods study utilizing surveys (n = 96) and semistructured interviews (n = 20) was conducted among a convenience sample of inpatients and outpatients at the Pengkalan Chepa C&C Center in Kelantan.
    RESULTS: Survey results showed that 89.6% of participants met screening criteria for moderate to severe addiction severity. Nearly 90% reported lifetime illicit amphetamine (syabu, meth, ice, and pil kuda) use, followed by alcohol (60.4%) and opioids (52.1%). Qualitative results pointed to the powerful influence of peer networks in drug initiation and relapse, and the positive effect of the C&C center on drug rehabilitation.
    CONCLUSIONS: The drug use profile of the Kelantan C&C center enrollees shows extensive pretreatment amphetamine use, polysubstance use, and injection drug use, including high-risk behaviors such as sharing needles, syringes, and containers. Evidence points to the need for integration of social support-oriented practices and behavioral interventions into the rehabilitation of drug users in this region.
    Study site: Pengkalan Chepa C&C Center, Jalan Padang Tembak, Kelantan, Malaysia
    MeSH terms: Adult; Ambulatory Care; Female; Humans; Malaysia/epidemiology; Male; Peer Group; Risk-Taking; Substance Abuse, Intravenous/epidemiology*; Substance Abuse Treatment Centers; Substance Abuse Treatment Centers/statistics & numerical data*; Needle Sharing/statistics & numerical data; Substance-Related Disorders/epidemiology*; Young Adult
  13. Dominic JA, Aris AZ, Sulaiman WN, Tahir WZ
    Environ Monit Assess, 2016 Mar;188(3):191.
    PMID: 26914327 DOI: 10.1007/s10661-016-5192-8
    The approach of this paper is to predict the sand mass distribution in an urban stormwater holding pond at the Stormwater Management And Road Tunnel (SMART) Control Centre, Malaysia, using simulated depth average floodwater velocity diverted into the holding during storm events. Discriminant analysis (DA) was applied to derive the classification function to spatially distinguish areas of relatively high and low sand mass compositions based on the simulated water velocity variations at corresponding locations of gravimetrically measured sand mass composition of surface sediment samples. Three inflow parameter values, 16, 40 and 80 m(3) s(-1), representing diverted floodwater discharge for three storm event conditions were fixed as input parameters of the hydrodynamic model. The sand (grain size > 0.063 mm) mass composition of the surface sediment measured at 29 sampling locations ranges from 3.7 to 45.5%. The sampling locations of the surface sediment were spatially clustered into two groups based on the sand mass composition. The sand mass composition of group 1 is relatively lower (3.69 to 12.20%) compared to group 2 (16.90 to 45.55%). Two Fisher's linear discriminant functions, F 1 and F 2, were generated to predict areas; both consist of relatively higher and lower sand mass compositions based on the relationship between the simulated flow velocity and the measured surface sand composition at corresponding sampling locations. F 1 = -9.405 + 4232.119 × A - 1795.805 × B + 281.224 × C, and F 2 = -2.842 + 2725.137 × A - 1307.688 × B + 231.353 × C. A, B and C represent the simulated flow velocity generated by inflow parameter values of 16, 40 and 80 m(3) s(-1), respectively. The model correctly predicts 88.9 and 100.0% of sampling locations consisting of relatively high and low sand mass percentages, respectively, with the cross-validated classification showing that, overall, 82.8% are correctly classified. The model predicts that 31.4% of the model domain areas consist of high-sand mass composition areas and the remaining 68.6% comprise low-sand mass composition areas.
    MeSH terms: Environmental Monitoring/methods; Malaysia; Models, Theoretical; Water Movements*; Discriminant Analysis*; Ponds/analysis
  14. Nithyamathi K, Chandramathi S, Kumar S
    PLoS One, 2016;11(2):e0136709.
    PMID: 26914483 DOI: 10.1371/journal.pone.0136709
    BACKGROUND: One of the largest cross-sectional study in recent years was carried out to investigate the prevalence of intestinal parasitic infections among urban and rural school children from five states namely Selangor, Perak, Pahang, Kedah and Johor in Peninsula Malaysia. This information would be vital for school authorities to influence strategies for providing better health especially in terms of reducing intestinal parasitism.

    METHODS AND PRINCIPAL FINDINGS: A total of 3776 stool cups was distributed to 26 schools throughout the country. 1760 (46.61%) responded. The overall prevalence of intestinal parasitic infection in both rural and urban areas was 13.3%, with Blastocystis sp (10.6%) being the most predominant, followed by Trichuris trichiura (3.4%), Ascaris lumbricoides (1.5%) and hook worm infection (0.9%). Only rural school children had helminthic infection. In general Perak had the highest infection (37.2%, total, n = 317), followed by Selangor (10.4%, total, n = 729), Pahang (8.6%, total, n = 221), Kedah (6.2%, total, n = 195) and Johor (3.4%, total, n = 298). School children from rural schools had higher infection (13.7%, total, n = 922) than urban school children (7.2%, total, n = 838). Subtype (ST) 3 (54.3%) is the most predominant ST with persons infected with only ST1 and ST3 showing symptoms. Blastocystis sp infection significantly associated with low household income, low parent's education and presence of symptoms (p<0.05).

    CONCLUSION: It is critical that we institute deworming and treatment to eradicate the parasite especially in rural school children.

    MeSH terms: Child; Cross-Sectional Studies; Feces/parasitology; Female; Humans; Malaysia/epidemiology; Male; Risk Factors; Rural Health; Rural Population; Urban Health; Urban Population; Prevalence; Blastocystis Infections/epidemiology*; Blastocystis Infections/parasitology; Blastocystis Infections/prevention & control; Blastocystis/classification; Blastocystis/genetics; Blastocystis/isolation & purification
  15. S Gowda VB, Nagaiah BH, Sengodan B
    Biochem Mol Biol Educ, 2016 Mar 4;44(2):202-7.
    PMID: 26914989 DOI: 10.1002/bmb.20936
    Medical students build clinical knowledge on the grounds of previously obtained basic knowledge. The study aimed to evaluate the competency of third year medical students to interpret biochemically based clinical scenarios using knowledge and skills gained during year 1 and 2 of undergraduate medical training. Study was conducted on year 3 MBBS students at AIMST University, Malaysia. Clinical scenarios (25) were constructed and administered to student volunteers, making sure at least one question from each system of year 2 was represented. Feedback was obtained on a five-point Likert scale regarding perception of learning biochemistry in MBBS year 1 versus 2. Mean score of test was 18 (72.11%). Performance was comparatively better in questions related to topics learnt in year 1 and reinforced in year 2 compared to those learnt for first time in year 2. In the feedback obtained, 31% strongly agreed and 56% agreed understanding the subject was helped more by learning biochemistry in year 2 than in year 1. Likewise, 36% strongly agreed and 56% agreed appreciating the importance of biochemistry in patient diagnosis was helped more by learning biochemistry in year 2 than year 1. Thirty one percent strongly agreed and 54% agreed that year 1 biochemistry would have been more relevant if case discussions were done simultaneously. Students retain basic science subjects better and appreciate the importance of basic sciences in patient diagnosis if they are reinforced in the context of clinical situations. © 2016 by The International Union of Biochemistry and Molecular Biology, 44:202-207, 2016.
  16. Hassan NN, Plazzer JP, Smith TD, Halim-Fikri H, Macrae F, Zubaidi AA, et al.
    BMC Res Notes, 2016;9:125.
    PMID: 26915360 DOI: 10.1186/s13104-015-1798-0
    Databases for gene variants are very useful for sharing genetic data and to facilitate the understanding of the genetic basis of diseases. This report summarises the issues surrounding the development of the Malaysian Human Variome Project Country Node. The focus is on human germline variants. Somatic variants, mitochondrial variants and other types of genetic variation have corresponding databases which are not covered here, as they have specific issues that do not necessarily apply to germline variations.
  17. Anukulthanakorn K, Parhar IS, Jaroenporn S, Kitahashi T, Watanbe G, Malaivijitnond S
    Phytother Res, 2016 Jun;30(6):929-39.
    PMID: 26915634 DOI: 10.1002/ptr.5595
    We determined the neurotherapeutic effects of Pueraria mirifica extract (PME) and pure puerarin (PU) in comparison with 17β-estradiol (E2 ) in early- and late-stage cognitive impaired rats. Rats were ovariectomized (OVX), kept for 2 and 4 months to induce early- and late-stage cognitive impairment, respectively, and divided into four groups that were treated daily with (i) distilled water, (ii) 100 mg/kg of PME, (iii) 7 mg/kg of PU, and (iv) 80 µg/kg of E2 for 4 months. The estrogen deficiency symptoms of OVX rats were abrogated by treatment with E2 or PME, but not by treatment with PU. The mRNA level of genes associated with amyloid production (App and Bace1) and hyperphosphorylated Tau (Tau4) were upregulated together with the level of impaired cognition in the 2- and 4-month OVX rats. Treatment with E2 reduced the level of cognitive impairment more than that with PME and PU, and 2-month OVX rats were more responsive than 4-month OVX rats. All treatments down-regulated the Bace1 mRNA level in 2-month OVX rats, while PU and PME also decreased the App mRNA level in 2- and 4-month OVX rats, respectively. Only PU suppressed Tau4 expression in 2-month OVX rats. Thus, PME and PU elicit neurotherapeutic effects in different pathways, and earlier treatment is optimal. Copyright © 2016 John Wiley & Sons, Ltd.
    MeSH terms: Animals; Cognition Disorders/drug therapy*; Disease Models, Animal; Female; Plant Extracts/chemistry*; Rats, Sprague-Dawley; Pueraria/chemistry*; Rats
  18. Muniandy Narayanan J, Jesudoss VA
    Gen. Physiol. Biophys., 2016 Apr;35(2):185-94.
    PMID: 26915720 DOI: 10.4149/gpb_2015041
    Overconsumption of fructose increases the risk of nonalcoholic fatty liver disease (NAFLD), obesity and metabolic syndrome. NAFLD is currently one of the most common etiologies of chronic liver disease worldwide. The aim of the present study is to evaluate the hepatoprotective potential of zingerone against fructose-enriched diet-induced rat model of nonalcoholic fatty liver disease. Male albino Wistar rats were used and randomly divided into four groups: group 1, control rats fed with standard pellet; group 2, rats were fed normal pellet with intragastric intubation of zingerone (100 mg/kg/day); group 3, rats were fed fructose enriched diet alone; group 4, rats were fed fructose enriched diet with intragastric intubation of zingerone (100 mg/kg/day). Body weight, abdominal circumference, blood glucose, lipid profile and hepatic function indicators were increased and HDL reduced in group 3 rats. Liver pathology of group 3 showed marked changes which includes micro- and macrovesicular steatosis, marked inflammatory cell infiltration, sinusoidal fibrosis and with a significant increase in the area percentage of the collagen. Administration of zingerone reversed the fructose enriched diet induced changes especially body weight, abdominal circumference, blood glucose, lipid profile, hepatic function indicators and restored pathological alteration of liver. Taken together these data provide new insights into the preventive approach of zingerone against the development of the NAFLD.
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