Browse publications by year: 2016

  1. Sabuti AA, Mohamed CA
    Environ Sci Pollut Res Int, 2016 Sep;23(18):18451-65.
    PMID: 27287490 DOI: 10.1007/s11356-016-7023-4
    Concentration activities of (210)Pb and (210)Po in the PM10 were determined to discuss their distribution and chemical behavior in relation to meteorological parameters especially in air mass transport during monsoon events. Marine aerosol samples were collected between January 2009 and December 2010 at the coastal region of Mersing, which is located in the southern South China Sea and is about 160 km northeast of Johor Bahru, as part of the atmosphere-ocean interaction program in Malaysia. About 47 PM10 samples were collected using the Sierra-Andersen model 1200 PM10 sampler over a 2-year sampling campaign between January 2009 and December 2010. Samples were processed using acid digestion sequential extraction techniques to analyze various fractions such as Fe and Mn oxides, organic matter, and residual fractions. While, (210)Pb and (210)Po activities were measured with the Gross Alpha/Beta Counting System model XLB-5 Tennelec® Series 5 and the Alpha Spectrometry (model Alpha Analyst Spectroscopy system with a silicon-surface barrier detector), respectively. The distribution activities of (210)Pb and (210)Po in the PM10 samples were varied from 162 to 881 μBq/m(3) with mean value of 347 ± 170 μBq/m(3) and from 85 to 1009 μBq/m(3) with mean value of 318 ± 202 μBq/m(3), respectively. The analysis showed that (210)Po activity in our samples lies in a border and higher range than global distribution values due to contributions from external sources injected to the atmosphere. The speciation of (210)Pb and (210)Po in marine aerosol corresponds to transboundary haze; e.g., biomass burning especially forest fires and long-range air mass transport of terrestrial dust has enriched concentrations of particle mass in the local atmosphere. The monsoon seems to play an important role in transporting terrestrial dust from Indo-China and northern Asia especially during the northeast monsoon, as well as biogenic pollutants originating from Sumatra and the southern ASEAN region during southwest monsoon events.
    MeSH terms: Malaysia; Biomass
  2. Nikolopoulos GK, Kostaki EG, Paraskevis D
    Infect Genet Evol, 2016 Dec;46:256-268.
    PMID: 27287560 DOI: 10.1016/j.meegid.2016.06.017
    HIV strains continuously evolve, tend to recombine, and new circulating variants are being discovered. Novel strains complicate efforts to develop a vaccine against HIV and may exhibit higher transmission efficiency and virulence, and elevated resistance to antiretroviral agents. The United Nations Joint Programme on HIV/AIDS (UNAIDS) set an ambitious goal to end HIV as a public health threat by 2030 through comprehensive strategies that include epidemiological input as the first step of the process. In this context, molecular epidemiology becomes invaluable as it captures trends in HIV evolution rates that shape epidemiological pictures across several geographical areas. This review briefly summarizes the molecular epidemiology of HIV among people who inject drugs (PWID) in Europe and Asia. Following high transmission rates of subtype G and CRF14_BG among PWID in Portugal and Spain, two European countries, Greece and Romania, experienced recent HIV outbreaks in PWID that consisted of multiple transmission clusters including subtypes B, A, F1, and recombinants CRF14_BG and CRF35_AD. The latter was first identified in Afghanistan. Russia, Ukraine, and other Former Soviet Union (FSU) states are still facing the devastating effects of epidemics in PWID produced by AFSU (also known as IDU-A), BFSU (known as IDU-B), and CRF03_AB. In Asia, CRF01_AE and subtype B (Western B and Thai B) travelled from PWID in Thailand to neighboring countries. Recombination hotspots in South China, Northern Myanmar, and Malaysia have been generating several intersubtype and inter-CRF recombinants (e.g. CRF07_BC, CRF08_BC, CRF33_01B etc.), increasing the complexity of HIV molecular patterns.
    MeSH terms: Asia/epidemiology; Europe/epidemiology; Humans; Molecular Epidemiology
  3. Shan GW, Makmor-Bakry M, Omar MS
    Psychiatr Danub, 2016 Jun;28(2):146-53.
    PMID: 27287789
    Lithium has been the gold standard in treating bipolar disorder. In recent years, the use of lithium seems to be diminished although it is well tolerated among the bipolar disorder patients.
  4. Ngeow WC, Lim D
    Adv Ther, 2016 Jul;33(7):1105-39.
    PMID: 27287853 DOI: 10.1007/s12325-016-0357-y
    INTRODUCTION: The use of corticosteroids to reduce the post-operative sequelae of lower third molar surgery, namely pain, swelling and trismus, has been well studied by many researchers over the past 6 decades. This study reviewed the reported outcome of corticosteroids used in controlling the above sequalae after third molar surgery.

    MATERIALS AND METHODS: A PubMed, Medline, EMBASE and Google search was undertaken of all controlled clinical trials on the effects of corticosteroids on pain, swelling and trismus after lower third molar surgery. The review was limited to studies published over the last 10 years (2006-2015).

    RESULTS: Of the 46 initially retrieved articles, 34 were finally included. Eleven studies compared the effect of 2 similar (but different dose) or different group of corticosteroids. Thirty-one studies reported the effects of corticosteroids on all sequale, 2 reported the outcome on swelling and trismus and another 1 on swelling and pain only. In 16 of the studies, corticosteroid use resulted in significant reductions in pain after third molar removal. Twenty-two out of 29 studies reported reduced swelling against negative control while 18 out of 25 studies reported improved mouth opening. Fourteen studies reported the benefit of corticosteroids on all 3 sequelae, with 71.4% resulted from the use of methylprednisolone.

    CONCLUSION: Although there are some conflicting effects, the results of this analysis shows in general the benefits derived from short-term use of corticosteroids in relation to pain, swelling and trismus following third molar surgical extraction, with no side effects observed.

    FUNDING: This work was supported by the University of Malaya's High Impact Research grant UM.C/625/1/HIR/MOHE/05.

    MeSH terms: Adrenal Cortex Hormones/administration & dosage*; Edema/prevention & control*; Humans; Molar, Third/surgery*; Pain, Postoperative/drug therapy*; Postoperative Complications/prevention & control*; Tooth Extraction/methods; Trismus/prevention & control*
  5. Goh SY, Ang E, Bajpai S, Deerochanawong C, Hong EG, Hussein Z, et al.
    J Diabetes Complications, 2016 08;30(6):973-80.
    PMID: 27288201 DOI: 10.1016/j.jdiacomp.2016.05.019
    MeSH terms: Asia; Diabetes Mellitus/drug therapy*; Humans; Insulin/therapeutic use*; Patient-Centered Care*
  6. Dianita R, Jantan I, Jalil J, Amran AZ
    Phytomedicine, 2016 Jul 15;23(8):810-7.
    PMID: 27288916 DOI: 10.1016/j.phymed.2016.04.004
    BACKGROUND: Previous studies on Labisia pumila var. alata (LPva) have showed that it could inhibit low-density lipoprotein (LDL) oxidation and provide protection on myocardial infarction in rats.

    HYPOTHESIS/PURPOSE: We hypothesized that LPva extracts can modulate the lipid profiles and serum antioxidant status of hypercholesterolemic rats. In the present study, we investigated the effects of aqueous and 80% ethanol extracts of LPva on atherogenic and serum antioxidant parameters as well as changes in abdominal aorta of high-cholesterol diet rats.

    METHODS: The major components of the extracts, gallic acid, flavonoids and alkyl resorcinols were analyzed by using a validated reversed phase HPLC method. The rats were induced to hypercholesterolemic status with daily intake of 2% cholesterol for a duration of 8 weeks. Three different doses (100, 200 and 400mg/kg) of the extracts were administered daily on the 4th week onwards. The rats were then sacrificed and the blood was collected via abdominal aorta and serum was separated by centrifugation for biochemical analysis. Part of the aorta tissues were excised immediately for histopathological examination.

    RESULTS: The serum of LPva treated rats showed significant reduction in serum aspartate aminotransferase (AST), alanine aminotransferase (ALT) and lactate dehydrogenase (LDH) levels and the abdominal aorta showed a significant decrease of atheroma lesions in treated rats. Serum lipid profiles of treated rats showed a decrease in total cholesterol, total triglycerides and low-density lipoprotein (LDL) levels as compared to control group. The atherogenic indices in treated rats were significantly improved along with an increasing level of serum high-density lipoprotein (HDL). The extracts also exhibited significant increase of antioxidant enzymes and decrease of MDA as a product of lipid peroxidation.

    CONCLUSION: LPva extracts can reduce the risk of dyslipidemia by improving the serum lipid profiles and modulating serum antioxidants.

    MeSH terms: Alanine Transaminase/blood; Animals; Hypolipidemic Agents/pharmacology*; Antioxidants/pharmacology*; Aorta, Abdominal/drug effects*; Aspartate Aminotransferases/blood; Cholesterol, Dietary/adverse effects*; Diet; Dose-Response Relationship, Drug; L-Lactate Dehydrogenase/blood; Lipids/blood*; Male; Plant Extracts/pharmacology*; Rats, Wistar; Primulaceae/chemistry*; Dyslipidemias/chemically induced; Dyslipidemias/drug therapy; Atherosclerosis/prevention & control; Rats
  7. Tang L, Leung YY
    Int J Oral Maxillofac Surg, 2016 Nov;45(11):1358-1365.
    PMID: 27289248 DOI: 10.1016/j.ijom.2016.05.021
    The purpose of this systematic review was to answer the clinical question "When should elective neck dissection be performed in maxillary gingival and alveolar squamous cell carcinoma with a cN0 neck?" A systematic review, designed in accordance with the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) statement, was conducted by two independent reviewers with three rounds of search and evaluation. Ten studies with 506 patients were included in the final review. The overall risk of cervical metastasis was 23.2% for those who did not receive an elective neck dissection (END), which was 3.4 times higher than that in the END group (6.8%). The 5-year survival rate was higher in those who had an END (80.3%) when compared to those who did not receive an END (67.4%). Overall, 14.1% of the cases with cN0 maxillary squamous cell carcinoma (SCC) presented with positive node(s) in pathological specimens after END. The risk of occult cervical metastasis in a cN0 maxillary SCC case with pathological stage pT1, pT2, pT3, and pT4 was 11.1%, 12.1%, 20%, and 36.1%, respectively. It is therefore concluded that END is recommended in patients with cN0 maxillary SCC, especially in stage T3 or T4 cases.
    MeSH terms: Alveolar Process/surgery*; Carcinoma, Squamous Cell/mortality; Carcinoma, Squamous Cell/surgery*; Gingival Neoplasms/surgery*; Humans; Lymphatic Metastasis; Maxillary Neoplasms/surgery*; Neoplasm Staging; Retrospective Studies; Time Factors; Survival Rate; Treatment Outcome; Elective Surgical Procedures; Neck Dissection/methods*; Neck Dissection/mortality
  8. Zahari Z, Lee CS, Ibrahim MA, Musa N, Mohd Yasin MA, Lee YY, et al.
    Drug Alcohol Depend, 2016 08 01;165:143-50.
    PMID: 27289271 DOI: 10.1016/j.drugalcdep.2016.05.028
    BACKGROUND: CYP2B6 polymorphisms contribute to inter-individual variations in pharmacokinetics of methadone. Increased pain sensitivity is frequently reported by opioid dependent patients on methadone maintenance therapy (MMT). It is possible, therefore, that genetic polymorphisms in CYP2B6, which affects the metabolism of methadone, influence pain sensitivity among patients on MMT. This study investigated CYP2B6 polymorphisms and pain sensitivity in this group.

    METHODS: The cold pressor pain responses of 148 opioid dependent patients receiving MMT were evaluated using the cold pressor test (CPT). DNA was extracted from whole blood and subjected to polymerase chain reaction (PCR)-genotyping.

    RESULTS: Of the 148 subjects, 77 (52.0%) were carriers of CYP2B6*6 allele. CYP2B6*6 allele carriers had shorter cold pain threshold and pain tolerance times than non-carriers of CYP2B6*6 allele (21.05s vs 33.69s, p=0.036 and 27.15s vs 44.51s, p=0.020, respectively). Pain intensity scores of the CYP2B6*6 allele carriers was 67.55, whereas that of the CYP2B6*6 allele non-carriers was 64.86 (p=0.352).

    CONCLUSION: Our study indicates that the CYP2B6*6 allele is associated with a lower pain threshold and lower pain tolerance among males with opioid dependence on MMT. The CYP2B6*6 allele may provide a mechanistic explanation for clinical observations of heightened pain sensitivity among opioid dependent patients receiving MMT.

    MeSH terms: Adult; Alleles; Analgesics, Opioid/therapeutic use; Genotype; Heterozygote; Humans; Male; Methadone/blood; Methadone/pharmacokinetics; Methadone/therapeutic use*; Opioid-Related Disorders/drug therapy; Pain/drug therapy; Pain/genetics*; Polymorphism, Genetic; Pain Threshold/drug effects*; Opiate Substitution Treatment*; Cytochrome P-450 CYP2B6/genetics*
  9. Praveena SM, Shamira SS, Ismail SNS, Aris AZ
    Mar Pollut Bull, 2016 Sep 15;110(1):609-612.
    PMID: 27289286 DOI: 10.1016/j.marpolbul.2016.06.024
    This pilot study aims to assess Escherichia coli (E. coli) contamination and its perceived health risks among beachgoers in ten tropical beach sands along Port Dickson coastline (Malaysia). This study also aims to determine the relationship between perceived health symptoms and tropical beach sand exposure behavior. The concentration of E. coli in tropical beach sand ranged from 60cfu/100g to 4113cfu/100g. E. coli contamination was the highest at Tanjung Gemuk (4113±30cfu/100g) and the lowest at Tanjung Tuan (60±15cfu/100g); the high level of contamination could be due to the location of the former at the sewage outlet of nearby hotels. Skin symptoms were the most predominant among the health symptoms indicated by beachgoers. Exposure duration was significantly correlated with the perceived health symptoms among beachgoers in the beaches studied.
    MeSH terms: Bathing Beaches/standards*; Environmental Monitoring/methods*; Escherichia coli/isolation & purification*; Feces/microbiology*; Humans; Malaysia; Pilot Projects; Recreation; Silicon Dioxide*; Colony Count, Microbial
  10. Kooijman AM, Bruin CJW, van de Craats A, Grootjans AP, Oostermeijer JGB, Scholten R, et al.
    Sci Total Environ, 2016 Oct 15;568:107-117.
    PMID: 27289393 DOI: 10.1016/j.scitotenv.2016.05.086
    Dune slacks are important habitats, with many endangered plant species. A series of eleven dune slacks of 1-42years old was studied in SW-Texel, the Netherlands, with the EU-habitat directive species Liparis loeselii present in all except the youngest and oldest. Analysis of aerial photographs revealed that new slacks are currently formed every 4-5years. In each slack, topsoil and vegetation data were collected in 2010 and 2014-2015. During succession, vegetation changed from brackish pioneer stages to dune slacks with L. loeselii and Parnassia palustris and ultimately grassland species. Differences between dune slacks and sampling periods were mostly significant. Herb cover and soil C increased with slack age, and over the five year study period, while bare sand, bulk density and pH decreased. The annual pH-decrease was 0.055 and 0.075 for pH-H2O and pH-KCl respectively, and annual C-increase 0.16% and 35gm(-2). Liparis loeselii was only present between pHH2O 5.8-7.5 and pHKCl 5.6-7.6, and only occurred at C-content below 4.3%. In lime-poor dunes, environmental conditions thus become unsuitable approximately 34years after the start of succession. In the dune slacks, Liparis loeselii established within 6years, showed peak values after 11-16years, and declined until conditions became unsuitable. Rejuvenation may occur after large storms with fresh sand deposits. However, even with further succession, the present populations are not endangered and probably last until 2040. With new dune slacks every 5years, L. loeselii occurs in approximately eight different dune slacks at the same time, ensuring viable populations also in the future. This shows that adverse effects of succession can be counteracted by dynamics on local and landscape scale.
    MeSH terms: Conservation of Natural Resources*; Netherlands; Ecosystem*; Orchidaceae/physiology*; Plant Dispersal*
  11. Ibitoye MO, Hamzaid NA, Hasnan N, Abdul Wahab AK, Islam MA, Kean VS, et al.
    Med Eng Phys, 2016 Aug;38(8):767-75.
    PMID: 27289541 DOI: 10.1016/j.medengphy.2016.05.012
    The interaction between muscle contractions and joint loading produces torques necessary for movements during activities of daily living. However, during neuromuscular electrical stimulation (NMES)-evoked contractions in persons with spinal cord injury (SCI), a simple and reliable proxy of torque at the muscle level has been minimally investigated. Thus, the purpose of this study was to investigate the relationships between muscle mechanomyographic (MMG) characteristics and NMES-evoked isometric quadriceps torques in persons with motor complete SCI. Six SCI participants with lesion levels below C4 [(mean (SD) age, 39.2 (7.9) year; stature, 1.71 (0.05) m; and body mass, 69.3 (12.9) kg)] performed randomly ordered NMES-evoked isometric leg muscle contractions at 30°, 60° and 90° knee flexion angles on an isokinetic dynamometer. MMG signals were detected by an accelerometer-based vibromyographic sensor placed over the belly of rectus femoris muscle. The relationship between MMG root mean square (MMG-RMS) and NMES-evoked torque revealed a very high association (R(2)=0.91 at 30°; R(2)=0.98 at 60°; and R(2)=0.97 at 90° knee angles; P<0.001). MMG peak-to-peak (MMG-PTP) and stimulation intensity were less well related (R(2)=0.63 at 30°; R(2)=0.67 at 60°; and R(2)=0.45 at 90° knee angles), although were still significantly associated (P≤0.006). Test-retest interclass correlation coefficients (ICC) for the dependent variables ranged from 0.82 to 0.97 for NMES-evoked torque, between 0.65 and 0.79 for MMG-RMS, and from 0.67 to 0.73 for MMG-PTP. Their standard error of measurements (SEM) ranged between 10.1% and 31.6% (of mean values) for torque, MMG-RMS and MMG-PTP. The MMG peak frequency (MMG-PF) of 30Hz approximated the stimulation frequency, indicating NMES-evoked motor unit firing rate. The results demonstrated knee angle differences in the MMG-RMS versus NMES-isometric torque relationship, but a similar torque related pattern for MMG-PF. These findings suggested that MMG was well associated with torque production, reliably tracking the motor unit recruitment pattern during NMES-evoked muscle contractions. The strong positive relationship between MMG signal and NMES-evoked torque production suggested that the MMG might be deployed as a direct proxy for muscle torque or fatigue measurement during leg exercise and functional movements in the SCI population.
    MeSH terms: Activities of Daily Living; Adult; Biomechanical Phenomena; Electric Stimulation*; Female; Humans; Isometric Contraction*; Male; Middle Aged; Signal Processing, Computer-Assisted; Spinal Cord Injuries/physiopathology*; Torque*; Quadriceps Muscle/physiopathology*; Mechanical Phenomena*
  12. Fong JFY, Chin SF, Ng SM
    Biosens Bioelectron, 2016 Nov 15;85:844-852.
    PMID: 27290666 DOI: 10.1016/j.bios.2016.05.087
    Carbon dots (CDs) that showed strong blue fluorescence were successfully synthesised from sodium alginate via furnace pyrolysis. The single step pyrolytic synthesis was simple to perform while yielded CDs with high photostability, good water solubility and minimum by-products. In order to design the probe with "turn-on" sensing capability, the CDs were screened against a series of metal cations to first "turn-off" the fluorescence. It was found that ferric ions (Fe(3+)) were most responsive and effective in quenching the fluorescence of CDs. Based on this observation, the conditioning of the probe was performed to ensure the fluorescence was completely quenched, while not overloading the system with Fe(3+). At the optimised condition, the CDs-Fe(3+) mixture served as a highly specific detection probe for ascorbic acid (AA). The analytical potential of the probe was evaluated and showed a good linear range of response for AA concentration of 24-40μg/mL. The selectivity study against other possible co-existing species was carried out and proved that our unique "turn-on" fluorescence signalling strategy was highly effective and selective towards AA as the target analyte. The probe was demonstrated for quantification of AA in real samples, which was the commercially available vitamin C supplement. The result showed good accuracy with minimum deviation from standard method adopted for validation purpose.
    MeSH terms: Ascorbic Acid/analysis*; Carbon/chemistry*; Cations/chemistry; Fluorescent Dyes/chemistry*; Iron/chemistry; Spectrometry, Fluorescence/methods; Vitamins/analysis*; Dietary Supplements/analysis; Quantum Dots/ultrastructure; Quantum Dots/chemistry*; Limit of Detection
  13. Kargarfard M, Lam ET, Shariat A, Asle Mohammadi M, Afrasiabi S, Shaw I, et al.
    Phys Sportsmed, 2016 09;44(3):208-16.
    PMID: 27291761 DOI: 10.1080/00913847.2016.1200442
    OBJECTIVES: Obesity prevalence has increased in Iranian adolescents in recent years. However, few studies have examined the impact of intervention programs on this health issue. The main objective of this study was to evaluate the effects of 8-week endurance training (ET) and high intensity interval training (HIIT) on intercellular adhesion molecule-1(ICAM-1) and vascular adhesion molecule-1(VCAM-1) levels among obese and normal-weight male adolescents.
    METHODS: Thirty obese and 30 normal-weight subjects were assigned to the ET, HIIT, or control group for eight weeks. Before and after the intervention, ICAM-1, VCAM-1, body weight, BMI, VO2max, and blood pressures were measured. SPSS (Version 21) was used for data analysis, and the significance level was set at p 
    MeSH terms: Adolescent; Child; Humans; Male; Obesity/blood*; Obesity/physiopathology*; Obesity/therapy; Oxygen Consumption/physiology; Physical Endurance/physiology*; Intercellular Adhesion Molecule-1/blood*; Vascular Cell Adhesion Molecule-1/blood*; Arterial Pressure/physiology*
  14. Machiela MJ, Zhou W, Karlins E, Sampson JN, Freedman ND, Yang Q, et al.
    Nat Commun, 2016 06 13;7:11843.
    PMID: 27291797 DOI: 10.1038/ncomms11843
    To investigate large structural clonal mosaicism of chromosome X, we analysed the SNP microarray intensity data of 38,303 women from cancer genome-wide association studies (20,878 cases and 17,425 controls) and detected 124 mosaic X events >2 Mb in 97 (0.25%) women. Here we show rates for X-chromosome mosaicism are four times higher than mean autosomal rates; X mosaic events more often include the entire chromosome and participants with X events more likely harbour autosomal mosaic events. X mosaicism frequency increases with age (0.11% in 50-year olds; 0.45% in 75-year olds), as reported for Y and autosomes. Methylation array analyses of 33 women with X mosaicism indicate events preferentially involve the inactive X chromosome. Our results provide further evidence that the sex chromosomes undergo mosaic events more frequently than autosomes, which could have implications for understanding the underlying mechanisms of mosaic events and their possible contribution to risk for chronic diseases.
    MeSH terms: Aging/genetics*; Female; Humans; Male; Mosaicism*; Reproducibility of Results; Polymerase Chain Reaction; DNA Methylation/genetics; Chromosomes, Human, X/genetics*; X Chromosome Inactivation/genetics*
  15. Yik YI, Stathopoulos L, Hutson JM, Southwell BR
    Neuromodulation, 2016 Jul;19(5):515-21.
    PMID: 27293084 DOI: 10.1111/ner.12451
    AIM: As transcutaneous electrical stimulation (TES) increased defecation in children and adults with Slow-Transit Constipation (STC), we performed a pilot study to test if TES can improve symptoms (defecation and soiling) in children with chronic constipation without STC and transit delay in the anorectum.

    METHODS: Children with treatment-resistant constipation presenting to a tertiary hospital had gastrointestinal nuclear transit study (NTS) showing normal proximal colonic transit and anorectal holdup of tracer. TES was administered at home (1 hour/day for 3 months) using a battery-powered interferential stimulator, with four adhesive electrodes (4 × 4 cm) connected so currents cross within the lower abdomen at the level of S2-S4. Stimulation was added to existing laxatives. Daily continence diary, and quality-of-life questionnaires (PedsQL4.0) were compared before and after TES.

    RESULTS: Ten children (4 females: 5-10 years, mean 8 years) had holdup in the anorectum by NTS. Nine had <3 bowel motions (BM)/week. After three months TES, defecation frequency increased in 9/10 (mean 0.9-4.1 BM/week, p = 0.004), with 6/9 improved to ≥3 BM/week. Soiling reduced in 9/10 from 5.9 to 1.9 days/week with soiling, p = 0.004. Ten were on laxatives, and nine reduced/stopped laxative use. Quality-of-life improved to within the normal range.

    CONCLUSION: TES improved symptoms of constipation in >50% of children with treatment-resistant constipation with isolated holdup in the anorectum. Further studies (RCTs) are warranted in these children.

    MeSH terms: Child; Child, Preschool; Constipation/psychology; Constipation/therapy*; Transcutaneous Electric Nerve Stimulation/methods*; Female; Home Care Services; Humans; Male; Pilot Projects; Quality of Life; Outcome Assessment (Health Care)
  16. Dongare S, Gupta SK, Mathur R, Saxena R, Mathur S, Agarwal R, et al.
    Mol Vis, 2016;22:599-609.
    PMID: 27293376
    PURPOSE: Diabetic retinopathy is a common microvascular complication of long-standing diabetes. Several complex interconnecting biochemical pathways are activated in response to hyperglycemia. These pathways culminate into proinflammatory and angiogenic effects that bring about structural and functional damage to the retinal vasculature. Since Zingiber officinale (ginger) is known for its anti-inflammatory and antiangiogenic properties, we investigated the effects of its extract standardized to 5% 6-gingerol, the major active constituent of ginger, in attenuating retinal microvascular changes in rats with streptozotocin-induced diabetes.

    METHODS: Diabetic rats were treated orally with the vehicle or the ginger extract (75 mg/kg/day) over a period of 24 weeks along with regular monitoring of bodyweight and blood glucose and weekly fundus photography. At the end of the 24-week treatment, the retinas were isolated for histopathological examination under a light microscope, transmission electron microscopy, and determination of the retinal tumor necrosis factor-α (TNF-α), nuclear factor-kappa B (NF-κB), and vascular endothelial growth factor (VEGF) levels.

    RESULTS: Oral administration of the ginger extract resulted in significant reduction of hyperglycemia, the diameter of the retinal vessels, and vascular basement membrane thickness. Improvement in the architecture of the retinal vasculature was associated with significantly reduced expression of NF-κB and reduced activity of TNF-α and VEGF in the retinal tissue in the ginger extract-treated group compared to the vehicle-treated group.

    CONCLUSIONS: The current study showed that ginger extract containing 5% of 6-gingerol attenuates the retinal microvascular changes in rats with streptozotocin-induced diabetes through anti-inflammatory and antiangiogenic actions. Although precise molecular targets remain to be determined, 6-gingerol seems to be a potential candidate for further investigation.

    MeSH terms: Administration, Oral; Animals; Anti-Inflammatory Agents/pharmacology*; Blood Glucose/metabolism; Catechols/pharmacology*; Diabetes Mellitus, Experimental/blood; Diabetes Mellitus, Experimental/drug therapy; Diabetic Retinopathy/blood; Diabetic Retinopathy/drug therapy*; Enzyme-Linked Immunosorbent Assay; Fatty Alcohols/pharmacology*; Female; Immunohistochemistry; Male; Phosphorylation; Retinal Vessels/drug effects*; Retinal Vessels/ultrastructure; Tumor Necrosis Factor-alpha/metabolism; Blotting, Western; Retinal Neovascularization/blood; Retinal Neovascularization/prevention & control*; NF-kappa B/metabolism; Rats, Wistar; Angiogenesis Inhibitors/pharmacology*; Ginger/chemistry*; Vascular Endothelial Growth Factor A/blood; Rats
  17. Sivan PP, Syed S, Mok PL, Higuchi A, Murugan K, Alarfaj AA, et al.
    Stem Cells Int, 2016;2016:8304879.
    PMID: 27293447 DOI: 10.1155/2016/8304879
    Sustenance of visual function is the ultimate focus of ophthalmologists. Failure of complete recovery of visual function and complications that follow conventional treatments have shifted search to a new form of therapy using stem cells. Stem cell progenitors play a major role in replenishing degenerated cells despite being present in low quantity and quiescence in our body. Unlike other tissues and cells, regeneration of new optic cells responsible for visual function is rarely observed. Understanding the transcription factors and genes responsible for optic cells development will assist scientists in formulating a strategy to activate and direct stem cells renewal and differentiation. We review the processes of human eye development and address the strategies that have been exploited in an effort to regain visual function in the preclinical and clinical state. The update of clinical findings of patients receiving stem cell treatment is also presented.
    MeSH terms: Cell Self Renewal; Cell Differentiation; Eye; Humans; Regeneration; Stem Cells; Transcription Factors; Ophthalmologists
  18. Lai MM, Lein SY, Lau SH, Lai ML
    J Aging Res, 2016;2016:2052380.
    PMID: 27293889 DOI: 10.1155/2016/2052380
    This paper empirically tested eight key features of WHO guidelines to age-friendly community by surveying 211 informal caregivers and 402 self-care adults (aged 45 to 85 and above) in Malaysia. We examined the associations of these eight features with active aging and social connectedness through exploratory and confirmatory factor analyses. A structural model with satisfactory goodness-of-fit indices (CMIN/df = 1.11, RMSEA = 0.02, NFI = 0.97, TLI = 1.00, CFI = 1.00, and GFI = 0.96) indicates that transportation and housing, community support and health services, and outdoor spaces and buildings are statistically significant in creating an age-friendly environment. We found a statistically significant positive relationship between an age-friendly environment and active aging. This relationship is mediated by social connectedness. The results indicate that built environments such as accessible public transportations and housing, affordable and accessible healthcare services, and elderly friendly outdoor spaces and buildings have to be put into place before social environment in building an age-friendly environment. Otherwise, the structural barriers would hinder social interactions for the aged. The removal of the environmental barriers and improved public transportation services provide short-term solutions to meet the varied and growing needs of the older population.
  19. Ishak NA, Zahari Z, Justine M
    Scientifica (Cairo), 2016;2016:3230427.
    PMID: 27293970 DOI: 10.1155/2016/3230427
    Objective. To determine the effect of strengthening exercises for older people with low back pain (LBP). Methods. This study is a systematic review of experimental study which evaluated the evidence regarding exercises for older people with LBP by using EBSCO Academic Search Premier, EBSCO EconLit, Science Direct, PUBMED, and PEDro from 2006 to 2016. Search strategy for each database was conducted by using keywords such as "low back pain", "older people", and "strengthening exercise". Boolean operators were used to combine keywords and manual exclusion was conducted to verify studies which met the inclusion criteria. The articles reviewed were evaluated and critically appraised by using PEDro scale and SPSS version 20 was used to analyze the data. Results. Three articles were found regarding strengthening exercise for older people with LBP whereas one study was conducted on multicomponent exercise. The mean, standard deviation, and variance of the PEDro score of all the studies were 5.67, 2.33, and 1.528, respectively. Overall, the qualities of all studies reviewed were fair. Two articles showed significant results when compared to control group (p < 0.05). Conclusions. Strengthening exercise is a beneficial treatment for older people with LBP in reducing pain intensity, disability, and improved functional performances.
    MeSH terms: Low Back Pain*
  20. Afroz R, Tanvir EM, Karim N, Hossain MS, Alam N, Gan SH, et al.
    Biomed Res Int, 2016;2016:6437641.
    PMID: 27294126 DOI: 10.1155/2016/6437641
    The present study was designed to investigate the cardioprotective effects of Sundarban honey (SH) in rats with isoproterenol- (ISO-) induced myocardial infarction. Adult male Wistar Albino rats were pretreated with Sundarban honey (5 g/kg) daily for a period of 6 weeks. After the treatment period, ISO (85 mg/kg) was subcutaneously injected into the rats at 24 h intervals for 2 days. ISO-induced myocardial damage was indicated by increased serum cardiac specific troponin I levels and cardiac marker enzyme activities including creatine kinase-MB, lactate dehydrogenase, aspartate transaminase, and alanine transaminase. Significant increases in serum total cholesterol, triglycerides, and low-density lipoprotein-cholesterol levels were also observed, along with a reduction in the serum high-density lipoprotein-cholesterol level. In addition to these diagnostic markers, the levels of lipid peroxide products were significantly increased. The activities of antioxidant enzymes such as superoxide dismutase, glutathione peroxidase, and glutathione reductase were significantly decreased in the hearts after ISO-induced myocardial infarction. However, pretreatment of ischemic rats with Sundarban honey brought the biochemical parameters to near normalcy, indicating the protective effect of Sundarban honey against ISO-induced ischemia in rats. Histopathological findings of the heart tissues further confirmed the biochemical findings, indicating that Sundarban honey confers protection against ISO-induced oxidative stress in the myocardium.
    MeSH terms: Alanine Transaminase/metabolism; Animals; Antioxidants/metabolism; Aspartate Aminotransferases/metabolism; Body Weight; Free Radicals; Honey*; Isoproterenol/adverse effects*; L-Lactate Dehydrogenase/metabolism; Male; Myocardial Infarction/chemically induced*; Myocardial Infarction/prevention & control*; Myocardium/enzymology; Rats, Wistar; Reactive Oxygen Species/metabolism; Oxidative Stress; Troponin I/metabolism; Rats; Creatine Kinase, MB Form/metabolism
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