Browse publications by year: 2017

  1. Jalaei B, Zakaria MN, Mohd Azmi MH, Nik Othman NA, Sidek D
    Ann Otol Rhinol Laryngol, 2017 Apr;126(4):290-295.
    PMID: 28177264 DOI: 10.1177/0003489417690169
    OBJECTIVES: Gender disparities in speech-evoked auditory brainstem response (speech-ABR) outcomes have been reported, but the literature is limited. The present study was performed to further verify this issue and determine the influence of head size on speech-ABR results between genders.

    METHODS: Twenty-nine healthy Malaysian subjects (14 males and 15 females) aged 19 to 30 years participated in this study. After measuring the head circumference, speech-ABR was recorded by using synthesized syllable /da/ from the right ear of each participant. Speech-ABR peaks amplitudes, peaks latencies, and composite onset measures were computed and analyzed.

    RESULTS: Significant gender disparities were noted in the transient component but not in the sustained component of speech-ABR. Statistically higher V/A amplitudes and less steeper V/A slopes were found in females. These gender differences were partially affected after controlling for the head size.

    CONCLUSIONS: Head size is not the main contributing factor for gender disparities in speech-ABR outcomes. Gender-specific normative data can be useful when recording speech-ABR for clinical purposes.

    MeSH terms: Acoustic Stimulation/methods; Adult; Female; Humans; Malaysia; Male; Sex Factors; Speech*; Speech Perception/physiology*; Evoked Potentials, Auditory, Brain Stem/physiology*; Young Adult; Healthy Volunteers
  2. Mohd-Sharif N, Shaibullah S, Givajothi V, Tan CS, Ho KL, Teh AH, et al.
    Acta Crystallogr F Struct Biol Commun, 2017 02 01;73(Pt 2):109-115.
    PMID: 28177322 DOI: 10.1107/S2053230X17001212
    TylP is one of five regulatory proteins involved in the regulation of antibiotic (tylosin) production, morphological and physiological differentiation in Streptomyces fradiae. Its function is similar to those of various γ-butyrolactone receptor proteins. In this report, N-terminally His-tagged recombinant TylP protein (rTylP) was overproduced in Escherichia coli and purified to homogeneity. The rTylP protein was crystallized from a reservoir solution comprising 34%(v/v) ethylene glycol and 5%(v/v) glycerol. The protein crystals diffracted X-rays to 3.05 Å resolution and belonged to the trigonal space group P3121, with unit-cell parameters a = b = 126.62, c = 95.63 Å.
    MeSH terms: Amino Acid Sequence; Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Bacterial Proteins/chemistry*; Cloning, Molecular; Crystallization; Escherichia coli/genetics; Escherichia coli/metabolism; Glycerol/chemistry; Plasmids/metabolism; Plasmids/chemistry; Receptors, GABA-A/genetics; Receptors, GABA-A/metabolism; Receptors, GABA-A/chemistry*; Recombinant Proteins/genetics; Recombinant Proteins/metabolism; Recombinant Proteins/chemistry; Streptomyces/metabolism; Streptomyces/chemistry*; X-Ray Diffraction; Gene Expression; Crystallography, X-Ray; Ethylene Glycol/chemistry; Protein Conformation, alpha-Helical
  3. Khalaf S, Ariffin Z, Husein A, Reza F
    J Prosthodont, 2017 Dec;26(8):664-669.
    PMID: 28177575 DOI: 10.1111/jopr.12460
    PURPOSE: To compare the adhesion of three microorganisms on modified and unmodified silicone elastomer surfaces with different surface roughnesses and porosities.

    MATERIALS AND METHODS: Candida albicans, Streptococcus mutans, and Staphylococcus aureus were incubated with modified and unmodified silicone groups (N = 35) for 30 days at 37°C. The counts of viable microorganisms in the accumulating biofilm layer were determined and converted to cfu/cm2 unit surface area. A scanning electron microscope (SEM) was used to evaluate the microbial adhesion. Statistical analysis was performed using t-test, one-way ANOVA, and post hoc tests as indicated.

    RESULTS: Significant differences in microbial adhesion were observed between modified and unmodified silicone elastomers after the cells were incubated for 30 days (p < 0.001). SEM showed evident differences in microbial adhesion on modified silicone elastomer compared with unmodified silicone elastomer.

    CONCLUSIONS: Surface modification of silicone elastomer yielding a smoother and less porous surface showed lower adhesion of different microorganisms than observed on unmodified surfaces.

    MeSH terms: Bacterial Adhesion*; Calcium Sulfate*; Candida albicans/physiology*; Cell Adhesion*; Dental Materials*; Materials Testing; Maxillofacial Prosthesis*; Prosthesis Design; Silicone Elastomers*; Staphylococcus aureus/physiology*; Streptococcus mutans/physiology*; Surface Properties; Coated Materials, Biocompatible
  4. Habib MA, Yuen GC, Othman F, Zainudin NN, Latiff AA, Ismail MN
    Biochem. Cell Biol., 2017 04;95(2):232-242.
    PMID: 28177774 DOI: 10.1139/bcb-2016-0144
    The natural rubber latex extracted from the bark of Hevea brasiliensis plays various important roles in today's modern society. Following ultracentrifugation, the latex can be separated into 3 layers: C-serum, lutoids, and rubber particles. Previous studies have shown that a large number of proteins are present in these 3 layers. However, a complete proteome for this important plant is still unavailable. Protein sequences have been recently translated from the completed draft genome database of H. brasiliensis, leading to the creation of annotated protein databases of the following H. brasiliensis biosynthetic pathways: photosynthesis, latex allergens, rubberwood formation, latex biosynthesis, and disease resistance. This research was conducted to identify the proteins contained within the latex by way of de novo sequencing from mass spectral data obtained from the 3 layers of the latex. Peptides from these proteins were fragmented using collision-induced dissociation, higher-energy collisional dissociation, and electron-transfer dissociation activation methods. A large percentage of proteins from the biosynthetic pathways (63% to 100%) were successfully identified. In addition, a total of 1839 unique proteins were identified from the whole translated draft genome database (AnnHBM).
    MeSH terms: Allergens/genetics; Allergens/immunology; Allergens/isolation & purification*; Amino Acid Sequence; Chemical Fractionation; Latex/chemistry*; Photosynthesis/genetics; Plant Diseases/genetics; Plant Diseases/immunology; Plant Proteins/genetics; Plant Proteins/immunology; Plant Proteins/isolation & purification*; Ultracentrifugation; Proteome/isolation & purification*; Plant Bark/chemistry; Hevea/genetics; Hevea/immunology; Hevea/chemistry*; Proteomics/methods; Molecular Sequence Annotation; Disease Resistance/genetics; Gene Ontology
  5. Chung PY
    Curr Drug Targets, 2017;18(4):414-420.
    PMID: 27758704 DOI: 10.2174/1389450117666161019102025
    Pseudomonas aeruginosa is the most common Gram-negative bacterium associated with nosocomial and life-threatening chronic infections in cystic fibrosis patients. This pathogen is wellknown for its ability to attach to surfaces of indwelling medical devices to form biofilms, which consist of a regular array of extracellular polymers. Tenaciously bound to the surface of devices and inherently resilient to antibiotic treatment, P. aeruginosa poses a serious threat in clinical medicine and contributes to the persistence of chronic infections. Studies on microbial biofilms in the past decade involved mainly the understanding of environment signals, genetic elements and molecular mechanisms in biofilm formation, tolerance and dispersal. The knowledge obtained from the studies of these mechanisms is crucial in the establishment of strategies to eradicate or to prevent biofilm formation. Currently, biofilm infections are usually treated with combinations of antibiotics and surgical removal, in addition to frequent replacement of the infected device. More recently, specific natural sources have been identified as antibiofilm agents against this pathogen. This review will highlight the recent progress made by plant-derived compounds against P. aeruginosa biofilm infections in both in vitro or in vivo models.
    MeSH terms: Animals; Anti-Bacterial Agents/pharmacology*; Anti-Bacterial Agents/therapeutic use; Cystic Fibrosis/complications; Humans; Plant Extracts/pharmacology*; Plant Extracts/therapeutic use; Pseudomonas aeruginosa/drug effects*; Pseudomonas aeruginosa/physiology; Pseudomonas Infections/drug therapy; Biofilms/drug effects
  6. Fong CY, Khine MM, Peter AB, Lim WK, Rozalli FI, Rahmat K
    J Clin Neurosci, 2017 Feb;36:73-75.
    PMID: 27887978 DOI: 10.1016/j.jocn.2016.10.050
    A 14-year-old girl presented with encephalopathy, delirium and ophthalmoplegia following a 3day history of high-grade fever. Brain MRI on day 6 of illness showed diffusion restricted ovoid lesion in the splenium of corpus callosum. Dengue virus encephalitis was diagnosed with positive PCR for dengue virus type-2 in both serum and cerebrospinal fluid. She made a complete recovery from day 10 of illness. Repeat brain MRI on day 12 of illness showed resolution of the splenial lesion. Serial diffusion tensor imaging (DTI) showed normal fractional anisotropy values on resolution of splenial lesion indicating that MERS was likely due to transient interstitial oedema with preservation of white matter tracts. This is the first reported case of MERS following dengue virus infection. It highlights the usefulness of performing serial DTI in understanding the underlying pathogenesis of MERS. Our case report widens the neurological manifestations associated with dengue infection and reiterates that patients with MERS should be managed supportively as the splenial white matter tracts are reversibly involved in MERS.
    MeSH terms: Adolescent; Dengue/complications*; Female; Humans; Magnetic Resonance Imaging; Spleen/pathology; Encephalitis, Viral/etiology; Encephalitis, Viral/pathology; Diffusion Tensor Imaging
  7. Radzuan MN, Banat IM, Winterburn J
    Bioresour Technol, 2017 Feb;225:99-105.
    PMID: 27888734 DOI: 10.1016/j.biortech.2016.11.052
    In this research we assess the feasibility of using palm oil agricultural refinery waste as a carbon source for the production of rhamnolipid biosurfactant through fermentation. The production and characterization of rhamnolipid produced by Pseudomonas aeruginosa PAO1 grown on palm fatty acid distillate (PFAD) under batch fermentation were investigated. Results show that P. aeruginosa PAO1 can grow and produce 0.43gL(-1) of rhamnolipid using PFAD as the sole carbon source. Identification of the biosurfactant product using mass spectrometry confirmed the presence of monorhamnolipid and dirhamnolipid. The rhamnolipid produced from PFAD were able to reduce surface tension to 29mNm(-1) with a critical micelle concentration (CMC) 420mgL(-1) and emulsify kerosene and sunflower oil, with an emulsion index up to 30%. Results demonstrate that PFAD could be used as a low-cost substrate for rhamnolipid production, utilizing and transforming it into a value added biosurfactant product.
    MeSH terms: Biotechnology/methods*; Carbon/metabolism; Emulsions/chemistry; Fatty Acids/metabolism; Fatty Acids/chemistry; Fermentation; Food-Processing Industry; Glycolipids/biosynthesis*; Glycolipids/metabolism; Glycolipids/chemistry; Industrial Waste; Kerosene; Micelles; Pseudomonas aeruginosa/metabolism*; Mass Spectrometry/methods; Surface Tension; Surface-Active Agents/metabolism; Surface-Active Agents/chemistry*
  8. Irish AB, Viecelli AK, Hawley CM, Hooi LS, Pascoe EM, Paul-Brent PA, et al.
    JAMA Intern Med, 2017 02 01;177(2):184-193.
    PMID: 28055065 DOI: 10.1001/jamainternmed.2016.8029
    Importance: Vascular access dysfunction is a leading cause of morbidity and mortality in patients requiring hemodialysis. Arteriovenous fistulae are preferred over synthetic grafts and central venous catheters due to superior long-term outcomes and lower health care costs, but increasing their use is limited by early thrombosis and maturation failure. ω-3 Polyunsaturated fatty acids (fish oils) have pleiotropic effects on vascular biology and inflammation and aspirin impairs platelet aggregation, which may reduce access failure.

    Objective: To determine whether fish oil supplementation (primary objective) or aspirin use (secondary objective) is effective in reducing arteriovenous fistula failure.

    Design, Setting, and Participants: The Omega-3 Fatty Acids (Fish Oils) and Aspirin in Vascular Access Outcomes in Renal Disease (FAVOURED) study was a randomized, double-blind, controlled clinical trial that recruited participants with stage 4 or 5 chronic kidney disease from 2008 to 2014 at 35 dialysis centers in Australia, Malaysia, New Zealand, and the United Kingdom. Participants were observed for 12 months after arteriovenous fistula creation.

    Interventions: Participants were randomly allocated to receive fish oil (4 g/d) or matching placebo. A subset (n = 406) was also randomized to receive aspirin (100 mg/d) or matching placebo. Treatment started 1 day prior to surgery and continued for 12 weeks.

    Main Outcomes and Measures: The primary outcome was fistula failure, a composite of fistula thrombosis and/or abandonment and/or cannulation failure, at 12 months. Secondary outcomes included the individual components of the primary outcome.

    Results: Of 1415 eligible participants, 567 were randomized (359 [63%] male, 298 [53%] white, 264 [47%] with diabetes; mean [SD] age, 54.8 [14.3] y). The same proportion of fistula failures occurred in the fish oil and placebo arms (128 of 270 [47%] vs 125 of 266 [47%]; relative risk [RR] adjusted for aspirin use, 1.03; 95% CI, 0.86-1.23; P = .78). Fish oil did not reduce fistula thrombosis (60 [22%] vs 61 [23%]; RR, 0.98; 95% CI, 0.72-1.34; P = .90), abandonment (51 [19%] vs 58 [22%]; RR, 0.87; 95% CI, 0.62-1.22; P = .43), or cannulation failure (108 [40%] vs 104 [39%]; RR, 1.03; 95% CI, 0.83-1.26; P = .81). The risk of fistula failure was similar between the aspirin and placebo arms (87 of 194 [45%] vs 83 of 194 [43%]; RR, 1.05; 95% CI, 0.84-1.31; P = .68).

    Conclusions and Relevance: Neither fish oil supplementation nor aspirin use reduced failure of new arteriovenous fistulae within 12 months of surgery.

    Trial Registration: anzctr.org.au Identifier: CTRN12607000569404.

    MeSH terms: Administration, Oral; Adult; Arteriovenous Shunt, Surgical*; Aspirin/therapeutic use*; Constriction, Pathologic/prevention & control*; Double-Blind Method; Female; Fibrinolytic Agents/therapeutic use*; Fish Oils/therapeutic use*; Graft Occlusion, Vascular/prevention & control*; Renal Dialysis*; Humans; Kidney Failure, Chronic/therapy*; Male; Middle Aged; Vascular Patency/drug effects*; Treatment Outcome; Dietary Supplements
  9. Islam MA, Ahmed MJ, Khanday WA, Asif M, Hameed BH
    Ecotoxicol Environ Saf, 2017 Apr;138:279-285.
    PMID: 28081490 DOI: 10.1016/j.ecoenv.2017.01.010
    Hydrothermal carbonization of biomass wastes presents a promising step in the production of cost-effective activated carbon. In the present work, mesoporous activated carbon (HAC) was prepared by the hydrothermal carbonization of rattan furniture wastes followed by NaOH activation. The textural and morphological characteristics, along with adsorption performance of prepared HAC toward methylene blue (MB) dye, were evaluated. The effects of common adsorption variables on performance resulted in a removal efficiency of 96% for the MB sample at initial concentration of 25mg/L, solution pH of 7, 30°C, and 8h. The Langmuir equation showed the best isotherm data correlation, with a maximum uptake of 359mg/g. The adsorbed amount versus time data was well fitted by a pseudo-second order kinetic model. The prepared HAC with a high surface area of 1135m(2)/g and an average pore size distribution of 35.5Å could be an efficient adsorbent for treatment of synthetic dyes in wastewaters.
    MeSH terms: Adsorption; Charcoal/chemistry*; Coloring Agents/chemistry*; Kinetics; Methylene Blue/chemistry*; Sodium Hydroxide; Water Purification/methods; Biomass; Arecaceae*
  10. Jee Keen Raymond W, Illias HA, Abu Bakar AH
    PLoS One, 2017;12(1):e0170111.
    PMID: 28085953 DOI: 10.1371/journal.pone.0170111
    Cable joint insulation breakdown may cause a huge loss to power companies. Therefore, it is vital to diagnose the insulation quality to detect early signs of insulation failure. It is well known that there is a correlation between Partial discharge (PD) and the insulation quality. Although many works have been done on PD pattern recognition, it is usually performed in a noise free environment. Also, works on PD pattern recognition in actual cable joint are less likely to be found in literature. Therefore, in this work, classifications of actual cable joint defect types from partial discharge data contaminated by noise were performed. Five cross-linked polyethylene (XLPE) cable joints with artificially created defects were prepared based on the defects commonly encountered on site. Three different types of input feature were extracted from the PD pattern under artificially created noisy environment. These include statistical features, fractal features and principal component analysis (PCA) features. These input features were used to train the classifiers to classify each PD defect types. Classifications were performed using three different artificial intelligence classifiers, which include Artificial Neural Networks (ANN), Adaptive Neuro-Fuzzy Inference System (ANFIS) and Support Vector Machine (SVM). It was found that the classification accuracy decreases with higher noise level but PCA features used in SVM and ANN showed the strongest tolerance against noise contamination.
    MeSH terms: Artificial Intelligence; Biometry; Humans; Patient Discharge; Fractals; Polyethylene; Principal Component Analysis; Support Vector Machine
  11. Rajoo Y, Ambu S, Lim YA, Rajoo K, Tey SC, Lu CW, et al.
    PLoS One, 2017;12(1):e0170174.
    PMID: 28095446 DOI: 10.1371/journal.pone.0170174
    Intestinal parasitic infections (IPIs) have been recognized as one of the most significant causes of illness among disadvantaged communities. Many studies have been conducted on the prevalence of IPIs in Malaysia. However, these studies mostly focused on the indigenous groups in Peninsular Malaysia. The present study was conducted to provide the current baseline data on prevalence of IPIs, anaemia, malnutrition and associated risk factors among the indigenous communities in Sarawak, situation at northwest Borneo island of Malaysia. A cross sectional study was conducted among the longhouses communities. Stool samples were obtained and examined for the presence of IPIs using microscopy technique. Haemoglobin measurement was done using a portable haemoglobin analyzer. Malnutrition (i.e., stunting, underweight and wasting) was assessed using the WHO Anthro software. Statistical analysis was carried out using SPSS software. A total of 341participants took part in this study. The overall prevalence of IPIs was 57.5%. Multivariate analysis indicated that the absence of toilets (OR = 1.6; 95% CI = 1.1-2.7; p = 0.002) and close contact with animals (OR = 1.8; 95% CI = 1.3-2.9; p = 0.027) as significant predictors for IPIs. The incidence of anaemia was 36.4%. The incidence of underweight, wasting and stunting were 22.2%, 5.6% and 35.4%, respectively. Multivariate analysis demonstrated that low level of parental education attainment (OR = 1.9; 95% CI = 1.2-3.0; p = 0.006) was identified as significant predictor for anaemia. The incidence of wasting was significantly associated with mild anaemia (OR = 1.2; 95% CI = 0.9-1.7; p = 0.024). Low household income was identified as significant predictor for stunting (OR = 2.1; 95% CI = 9.8-22.2; p = 0.001) and underweight (OR = 1.9; 95% CI = 5.6-18.7; p = 0.037), respectively. Essentially, the present study highlighted that intestinal parasitic infections, anaemia and malnutrition are still prevalent among rural indigenous community in Sarawak. Improvement of socioeconomic status, periodic mass deworming, iron supplementation and health education program should be included in the control and prevention of public health strategies.
    MeSH terms: Adolescent; Adult; Aged; Aged, 80 and over; Anemia/epidemiology*; Animals; Child; Child, Preschool; Cross-Sectional Studies; Female; Humans; Infant; Intestinal Diseases, Parasitic/epidemiology*; Intestinal Diseases, Parasitic/parasitology; Malaysia/epidemiology; Male; Middle Aged; Parasites/pathogenicity*; Risk Factors; Rural Population; Socioeconomic Factors; Prevalence; Malnutrition/epidemiology*; Population Groups; Young Adult; Neglected Diseases/epidemiology*
  12. Charara R, Forouzanfar M, Naghavi M, Moradi-Lakeh M, Afshin A, Vos T, et al.
    PLoS One, 2017;12(1):e0169575.
    PMID: 28095477 DOI: 10.1371/journal.pone.0169575
    The Eastern Mediterranean Region (EMR) is witnessing an increase in chronic disorders, including mental illness. With ongoing unrest, this is expected to rise. This is the first study to quantify the burden of mental disorders in the EMR. We used data from the Global Burden of Disease study (GBD) 2013. DALYs (disability-adjusted life years) allow assessment of both premature mortality (years of life lost-YLLs) and nonfatal outcomes (years lived with disability-YLDs). DALYs are computed by adding YLLs and YLDs for each age-sex-country group. In 2013, mental disorders contributed to 5.6% of the total disease burden in the EMR (1894 DALYS/100,000 population): 2519 DALYS/100,000 (2590/100,000 males, 2426/100,000 females) in high-income countries, 1884 DALYS/100,000 (1618/100,000 males, 2157/100,000 females) in middle-income countries, 1607 DALYS/100,000 (1500/100,000 males, 1717/100,000 females) in low-income countries. Females had a greater proportion of burden due to mental disorders than did males of equivalent ages, except for those under 15 years of age. The highest proportion of DALYs occurred in the 25-49 age group, with a peak in the 35-39 years age group (5344 DALYs/100,000). The burden of mental disorders in EMR increased from 1726 DALYs/100,000 in 1990 to 1912 DALYs/100,000 in 2013 (10.8% increase). Within the mental disorders group in EMR, depressive disorders accounted for most DALYs, followed by anxiety disorders. Among EMR countries, Palestine had the largest burden of mental disorders. Nearly all EMR countries had a higher mental disorder burden compared to the global level. Our findings call for EMR ministries of health to increase provision of mental health services and to address the stigma of mental illness. Moreover, our results showing the accelerating burden of mental health are alarming as the region is seeing an increased level of instability. Indeed, mental health problems, if not properly addressed, will lead to an increased burden of diseases in the region.
    MeSH terms: Adolescent; Adult; Age Factors; Aged; Aged, 80 and over; Mental Disorders/epidemiology*; Child; Child, Preschool; Female; Health Status*; Humans; Infant; Infant, Newborn; Life Expectancy; Male; Middle Aged; Sex Factors; Time Factors; Global Health*; Mediterranean Region/epidemiology; Young Adult
  13. Ansari M, Humagain B, Hassali MA
    Res Social Adm Pharm, 2017 02 01;13(3):658-659.
    PMID: 28162993 DOI: 10.1016/j.sapharm.2017.01.004
    MeSH terms: Drug Utilization/statistics & numerical data*; Humans; Nepal; Patents as Topic; Practice Patterns, Physicians'; Drug Prescriptions/statistics & numerical data*; World Health Organization; Drugs, Generic*
  14. Muthaian R, Pakirisamy RM, Parasuraman S, Raveendran R
    J Pharmacol Pharmacother, 2017 2 7;7(4):159-164.
    PMID: 28163536 DOI: 10.4103/0976-500X.195898
    OBJECTIVE: To investigate the association of hypertension coexisting with diabetes mellitus with oxidative stress and inflammation in the kidneys of streptozotocin (STZ)-induced diabetic rats.

    MATERIALS AND METHODS: Male Wistar rats were used for the experiments. Blood glucose (BG), urea, blood pressure (BP), and heart rate (HR) were analyzed before and 48 h after STZ injection. Further, these parameters were monitored up to 3 months of diabetes induction. Subsequently, the inflammatory markers (C-reactive protein, tumor necrosis factor-alpha, and nitrate) and oxidative stress markers were estimated after 3 months of diabetes induction in the kidney homogenate. Histological analysis of renal tissue was also carried out.

    RESULTS: Linear elevation of BG, urea, mean arterial pressure (MAP), and HR was observed up to 3 months of diabetes induction. In the same manner, inflammatory and oxidative stress markers were also found to be significantly increased. Notably, the histological analysis revealed the signs of nephropathy such as increased mesangial cell number, thickness of basement membrane, and renal artery. Inflammatory and oxidative stress markers positively correlated with elevated BP and BG, but the correlation was better with BP rather than BG.

    CONCLUSION: Hypertension has a strong implication in the increased oxidative stress and inflammation of diabetic kidney at the very early stage of diabetes mellitus.

    MeSH terms: Animals; Basement Membrane; Blood Glucose; Blood Pressure; Blood Pressure Determination; C-Reactive Protein; Diabetes Mellitus, Experimental; Heart Rate; Hypertension; Inflammation; Kidney; Kidney Diseases; Male; Renal Artery; Streptozocin; Tumor Necrosis Factor-alpha; Urea; Rats, Wistar; Oxidative Stress; Mesangial Cells; Rats
  15. Majeedkutty NA, Khairulanuar NA
    J Family Community Med, 2017 Jan-Apr;24(1):18-22.
    PMID: 28163571 DOI: 10.4103/2230-8229.197177
    BACKGROUND: Equestrian sport carries with it an implicit risk of injury. Despite the frequency of injuries in equestrian sports, there is no published study on injuries of equestrian athletes in Malaysia.
    OBJECTIVE: The objective of this study was to determine the prevalence of injuries and its correlates among horseback riders.
    SUBJECTS AND METHODS: A web-based standardized questionnaire was used to collect data for this cross-sectional survey. Horseback riders aged 18 years and above were included in the study. Out of 169 participants, 93 were females and 76 were males. The correlation of injuries to gender, age, level of experience, exercise habits, use of safety measures, and type of equestrian sport were determined. Chi-square test was performed to test for statistical significance.
    RESULTS: The prevalence was high with 85.8% of the participants reporting symptoms and characteristics of injuries in the past 12 months. The most frequently perceived symptoms reported were in the upper extremities (43.4%) followed by lower extremities (40.7%), head injury (8.3%) and injuries of upper and lower back (3.4%). There was a higher prevalence of injury among female participants (55.03%) than males (42.60%). A significant correlation was found between gender and prevalence of injuries. About 70% of the riders sustained soft tissue injuries. Fifty-five percent of the injured were involved in recreational riding. The most common mechanism of injury was a fall from a horse. Sixty percent of the injured riders did not seek medical attention after being injured, and physiotherapy consultation was even lower with 10.3%.
    CONCLUSIONS: The high prevalence of injuries and low rate of medical consultation emphasize the need for education programs on safety in Malaysia. Sessions should be held to improve coaching for riders and instructors, and their knowledge of the nature of the horse, mechanisms of injuries, horse handling, and riding skills to help them host safe equestrian activities.
    KEYWORDS: Equestrian; horse riding; injuries; prevalence; safety equipment
  16. Lim KH, Lim HL, Teh CH, Kee CC, Khoo YY, Ganapathy SS, et al.
    Tob Induc Dis, 2017;15:9.
    PMID: 28163668 DOI: 10.1186/s12971-016-0108-5
    BACKGROUND: A multitude of studies have revealed that smoking is a learned behaviour during adolescence and efforts to reduce the incidence of smoking has been identified as long-term measures to curb the smoking menace. The objective of this study was to determine the prevalence as well as the intra and inter-personal factors associated with smoking among upper secondary school students in selected schools in Peninsular Malaysia.

    METHODS: A study was carried out in 2013, which involved a total of 40 secondary schools. They were randomly selected using a two-stage clustering sampling method. Subsequently, all upper secondary school students (aged 16 to 17 years) from each selected school were recruited into the study. Data was collected using a validated standardised questionnaire.

    RESULTS: This study revealed that the prevalence of smoking was 14.6% (95% CI:13.3-15.9), and it was significantly higher among males compared to females (27.9% vs 2.4%, p 

    MeSH terms: Adolescent; Emotions; Female; Humans; Loneliness; Malaysia; Male; Parents; Surveys and Questionnaires; Schools; Students; Incidence; Prevalence; Tobacco Products
  17. Chin KY
    J Osteoporos, 2017;2017:3710959.
    PMID: 28163951 DOI: 10.1155/2017/3710959
    Aspirin is a cyclooxygenase inhibitor commonly used in primary prevention of cardiovascular diseases and cancers. Its users are elderly population susceptible to osteoporosis. It also inhibits the synthesis of prostaglandin E2 essential in bone remodeling. This prompts the question whether it can influence bone health among users. This review aimed to summarize the current literature on the use of aspirin on bone health. A literature search on experimental and clinical evidence on the effects of aspirin on bone health was performed using major scientific databases. In vitro studies showed that aspirin could enhance the survival of bone marrow mesenchymal stem cells, the progenitors of osteoblasts, and stimulate the differentiation of preosteoblasts. Aspirin also inhibited the nuclear factor kappa-B (NFκB) pathway and decreased the expression of receptor activator of NFκB ligand, thus suppressing the formation of osteoclast. Aspirin could prevent bone loss in animal models of osteoporosis. Despite a positive effect on bone mineral density, the limited human epidemiological studies revealed that aspirin could not reduce fracture risk. A study even suggested that the use of aspirin increased fracture risk. As a conclusion, aspirin may increase bone mineral density but its effect on fracture prevention is inconclusive. More data are needed to determine the effects of aspirin and bone health in human.
    MeSH terms: Animals; Aspirin*; Bone and Bones; Humans; Osteoporosis; Fractures, Bone
  18. Inoue T, Kainuma M, Baba K, Oshiro N, Kimura N, Chan EW
    J Intercult Ethnopharmacol, 2017 Jan 3;6(1):121-127.
    PMID: 28163970 DOI: 10.5455/jice.20161229060034
    In this short review, the current knowledge on the botany, ecology, uses, and medicinal properties of the multipurpose Garcinia subelliptica (Fukugi) is updated. As yet, there are no reviews on this indigenous and heritage coastal tree species of the Ryukyu Islands in Japan, which has ethnocultural, ecological, and pharmacological significance. Planted by the Okinawan people some 300 years ago, Fukugi trees serve as windbreaks and accord protection against the destructive typhoons. The species has become a popular ornamental tree, and its bark has been used for dyeing fabrics. It forms part of the food chain for mammals and insects and serves as nesting sites for birds. Endowed with bioactive compounds of benzophenones, xanthones, biflavonoids, and triterpenoids, G. subelliptica possesses anticancer, anti-inflammatory, anti-tyrosinase, trypanocidal, antibacterial, DNA topoisomerase inhibitory, DNA strand scission, choline acetyltransferase enhancing, hypoxia-inducible factor-1 inhibitory, and antiandrogenic activities. Fukugetin and fukugiside are two novel biflavonoids named after the species. The chemical constituents of Fukugi fruits when compared with those of mangosteen yielded interesting contrasts.
    MeSH terms: Animals; Anti-Bacterial Agents; Benzophenones; Birds; Botany; Choline O-Acetyltransferase; DNA; DNA Topoisomerases, Type I; Ecology; Fruit; Japan; Mammals; Trees; Monophenol Monooxygenase; Food Chain; Garcinia; Garcinia mangostana; Xanthones; Biflavonoids; Hypoxia-Inducible Factor 1; Cyclonic Storms; Islands
  19. Cheah HY, Kiew LV, Lee HB, Japundžić-Žigon N, Vicent MJ, Hoe SZ, et al.
    J Appl Toxicol, 2017 Nov;37(11):1268-1285.
    PMID: 28165137 DOI: 10.1002/jat.3437
    While nano-sized construct (NSC) use in medicine has grown significantly in recent years, reported unwanted side effects have raised safety concerns. However, the toxicity of NSCs to the cardiovascular system (CVS) and the relative merits of the associated evaluation methods have not been thoroughly studied. This review discusses the toxicological profiles of selected NSCs and provides an overview of the assessment methods, including in silico, in vitro, ex vivo and in vivo models and how they are related to CVS toxicity. We conclude the review by outlining the merits of telemetry coupled with spectral analysis, baroreceptor reflex sensitivity analysis and echocardiography as an appropriate integrated strategy for the assessment of the acute and chronic impact of NSCs on the CVS. Copyright © 2017 John Wiley & Sons, Ltd.
    MeSH terms: Algorithms; Animals; Blood Pressure/drug effects; Cardiovascular Diseases/chemically induced*; Cardiovascular Diseases/diagnosis*; Cardiovascular Diseases/physiopathology; Cardiovascular System/drug effects*; Cardiovascular System/physiopathology; Heart Rate/drug effects; Humans; Predictive Value of Tests; Risk Factors; Signal Processing, Computer-Assisted; Telemetry*; Baroreflex/drug effects*; Risk Assessment; Nanomedicine/methods*; Nanoparticles/toxicity*; Nanoparticles/chemistry
  20. Sawangjit R, Puttarak P, Saokaew S, Chaiyakunapruk N
    Phytother Res, 2017 Apr;31(4):555-567.
    PMID: 28165166 DOI: 10.1002/ptr.5783
    Cissus quadrangularis L. (Cissus) is a medicinal plant commonly used for centuries for various conditions, but lacks critical appraisal of its clinical effects. This study aimed to determine the efficacy and safety of Cissus in all conditions. Publications from 12 electronic databases were searched from inception through November 2016. A total of nine studies with 1108 patients were included. Each outcome was pooled using a random effects model. Effects of Cissus on hemorrhoid symptoms were not different from any comparators but had significant effects on bone pain. Effects of Cissus combination products on body weight reduction, low-density lipoprotein, triglyceride, total cholesterol, and fasting blood sugar were superior to placebo, with weighted mean difference of -5.19 kg (-8.82, -1.55), -14.43 mg/dl (-20.06, -8.80), -37.50 mg/dl (-48.71, -26.29), -50.50 mg/dl (-70.97, -30.04), and -10.39 mg/dl (-14.60, -6.18), respectively. No serious adverse effects were reported. Quality of evidence based on Grades of Recommendations Assessment Development and Evaluation (GRADE) indicated low (bone fractures) to high quality (hemorrhoids, body weight reduction).In conclusion, Cissus had benefit for bone fractures, but not for hemorrhoids. For obesity/overweight, only combination products are pooled and show benefit. However, high-quality studies remain needed. Copyright © 2017 John Wiley & Sons, Ltd.
    MeSH terms: Biological Products/chemistry*; Humans; Plant Extracts/chemistry*; Randomized Controlled Trials as Topic; Cissus/chemistry*
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