Browse publications by year: 2019

  1. Md Yusof A, Abd Ghafar N, Kamarudin TA, Chua KH, Azmi MF, Ng SL, et al.
    Cytotechnology, 2019 Dec;71(6):1121-1135.
    PMID: 31606844 DOI: 10.1007/s10616-019-00349-8
    This study evaluated the effects of Gelam honey (GH) on ex vivo corneal fibroblast ulcer model via wound healing assay, gene expression and immunocytochemistry. Corneal fibroblasts from New Zealand white rabbits were culture expanded. The corneal fibroblast wound healing capacity was observed by creating a circular wound onto confluent monolayer cells cultured in basal medium (BM), BM with GH, serum-enriched basal medium (BMS) and BMS with GH respectively. Wound healing assay and phenotypic characterization of the corneal fibroblast were performed at different stages of wound closure. Expression of aldehyde dehydrogenase (ALDH), vimentin, α-smooth muscle actin (α-SMA), lumican, collagen I and matrix metalloproteinase 12 (MMP 12) were measured at day 1, day 3 and complete wound closure day. Corneal fibroblast cultured in BMS with GH demonstrated the fastest wound closure, at day 5 post wounding. The gene expressions of ALDH and vimentin were higher than control groups while α-SMA expression was lower, in GH enriched media. The expressions of lumican, collagen I and MMP 12 were also higher in cells cultured in GH enriched media compared to the control groups. GH was shown to promote in vitro corneal fibroblast wound healing and may be a potential natural adjunct in the treatment of corneal wound.
    MeSH terms: Actins; Aldehyde Dehydrogenase; Animals; Cornea; Corneal Ulcer; Honey; Immunohistochemistry; Rabbits; Ulcer; Vimentin; Collagen Type I; Control Groups; Matrix Metalloproteinase 12; Corneal Injuries; Lumican
  2. Arai T, Taha H, Amalina R, Iizuka Y, Chang CW
    J Fish Biol, 2019 Dec;95(6):1506-1511.
    PMID: 31606890 DOI: 10.1111/jfb.14154
    Tenualosa ilisha was found recently in the Perak River in western Peninsular Malaysia. Molecular phylogenetic and haplotype network analyses suggest that T. ilisha has two genetically distinct populations/groups: (i) Peninsular Malaysia (Malaysia population), and (ii) Peninsular Malaysia, Thailand, India and Bangladesh (Indian Ocean population). The results also suggest that the T ilisha population in Peninsular Malaysia is genetically heterogeneous with a typical anadromous migration pattern.
    MeSH terms: Animals; Bangladesh; Fishes/genetics*; Genetics, Population*; India; Malaysia; Otolithic Membrane/chemistry*; Phylogeny*; Thailand; Indian Ocean; Animal Migration
  3. Kua CH, Mak VS, Lee SWH
    BMJ Open, 2019 10 11;9(10):e030106.
    PMID: 31604786 DOI: 10.1136/bmjopen-2019-030106
    OBJECTIVE: To examine the determinants of deprescribing among health professionals in nursing homes, focusing on knowledge, practice and attitude.

    DESIGN: This was a qualitative study comprising semi-structured face-to-face interviews guided by 10 open-ended questions. Interviews were conducted until data saturation was achieved and no new ideas were formed. The interviews were audio-recorded, transcribed verbatim and analysed for themes. To derive themes, we employed directed content analysis of transcript data. Coding was completed using a combination of open, axial and selective coding.

    SETTING: Four nursing homes in Singapore.

    PARTICIPANTS: The study involved 17 participants (comprising 4 doctors, 4 pharmacists and 9 nurses).

    RESULTS: Two key themes were identified, enablers and challenges. These were enablers and challenges faced by doctors, pharmacists and nurses towards deprescribing. The identified subthemes for enablers of deprescribing were: (1) awareness of medications that are unnecessary or could be targeted for deprescribing; (2) improving quality of life for patients with limited life expectancy; (3) improving communication between doctors, pharmacists and nurses; (4) systematic deprescribing practice and educational tools and (5) acknowledgement of possible benefits of deprescribing. The identified subthemes for challenges of deprescribing were: (1) symptoms not acknowledged as possibly drug-related; (2) lack of knowledge in patient's and family members' preferences; (3) lack of coordination between health professionals in hospitals and nursing homes and (4) limited tools for deprescribing. The development of a local guideline, mentoring nurses, case discussions, better shared decision-making and improving multidisciplinary communication, may help to support the process of deprescribing.

    CONCLUSION: In conclusion, this study highlighted that deprescribing in the nursing homes is perceived by health professionals to be challenging and future research could assess how routine case studies, mentoring and better multidisciplinary communication could improve deprescribing knowledge and process in the nursing homes.

    MeSH terms: Deprescriptions*; Attitude of Health Personnel*; Humans; Interviews as Topic; Health Knowledge, Attitudes, Practice*; Nurses; Nursing Homes*; Pharmacists; Physicians; Singapore; Qualitative Research
  4. Jainlabdin MH, Batra A, Sánchez Paredes E, Hernández Hernández F, Fu G, Tovar-Torres J
    Sci Rep, 2019 10 11;9(1):14692.
    PMID: 31604994 DOI: 10.1038/s41598-019-51198-6
    Invasive candidiasis is one of the most common nosocomial fungal infections worldwide. Delayed implementation of effective antifungal treatment caused by inefficient Candida diagnosis contributes to its notoriously high mortality rates. The availability of better Candida diagnostic tools would positively impact patient outcomes. Here, we report on the development of a single-tube, dual channel pentaplex molecular diagnostic assay based on Multiplex Probe Amplification (MPA) technology. It allows simultaneous identification of C. auris, C. glabrata and C. krusei, at species-level as well as of six additional albicans and non-albicans pathogenic Candida at genus level. The assay overcomes the one-channel one-biomarker limitation of qPCR-based assays. Assay specificities are conferred by unique biomarker probe pairs with characteristic melting temperatures; post-amplification melting curve analysis allows simple identification of the infectious agent. Alerting for the presence of C. auris, the well-characterised multi-drug resistant outbreak strain, will facilitate informed therapy decisions and aid antifungal stewardship. The MPA-Candida assay can also be coupled to a pan-Fungal assay when differentiation between fungal and bacterial infections might be desirable. Its multiplexing capacity, detection range, specificity and sensitivity suggest the potential use of this novel MPA-Candida assay in clinical diagnosis and in the control and management of hospital outbreaks.
    MeSH terms: Antifungal Agents/therapeutic use; Candida/classification; Candida/genetics*; DNA, Fungal/genetics; Humans; Sensitivity and Specificity; Biomarkers/analysis; Molecular Diagnostic Techniques/methods*; Drug Resistance, Multiple, Fungal; Transition Temperature; Limit of Detection; Candidiasis, Invasive/diagnosis*; Candidiasis, Invasive/drug therapy; Candidiasis, Invasive/microbiology; Multiplex Polymerase Chain Reaction/methods*; Real-Time Polymerase Chain Reaction/methods
  5. Rizk C, Long S, Okyar HB, Baradaran S, Al Fares E, Sangau JK, et al.
    Radiat Prot Dosimetry, 2019 Dec 31;187(4):418-425.
    PMID: 31605130 DOI: 10.1093/rpd/ncz182
    An intercomparison exercise (IC) on whole body dosemeters to determine the quantity personal dose equivalent Hp (10) in photon radiation fields was jointly organised and conducted by the International Atomic Energy Agency (IAEA) and the Australian Radiation Protection and Nuclear Safety Agency (ARPANSA) for individual monitoring services (IMS) in Asia and the Pacific region. This was arranged to help the IMS in the region to achieve a more accurate dosimetry service and to improve their performance. Twenty-four IMS participated in this IC. Four sets of dosemeters were irradiated using X-ray and gamma radiation qualities at 0° and 20° angle of incidence, respectively. All the IMS provided results that were within the acceptable limits defined by the IAEA. However, only a minority of participants reported confidence intervals that included the reference dose, for each exposure scenario. For few systems, the overall performance could be significantly improved by reviewing calibration procedures.
    MeSH terms: Asia; Australia; Humans; Nuclear Energy; Radiation Dosage; Radiation Monitoring/standards*; Radiation Protection/standards*; Whole-Body Counting/standards*; Occupational Exposure/analysis*; Occupational Exposure/prevention & control; Photons*; Radiation Dosimeters/standards*
  6. Lo SK, Liew CS, Tey KS, Mekhilef S
    Sensors (Basel), 2019 Oct 09;19(20).
    PMID: 31600904 DOI: 10.3390/s19204354
    The advancement of the Internet of Things (IoT) as a solution in diverse application domains has nurtured the expansion in the number of devices and data volume. Multiple platforms and protocols have been introduced and resulted in high device ubiquity and heterogeneity. However, currently available IoT architectures face challenges to accommodate the diversity in IoT devices or services operating under different operating systems and protocols. In this paper, we propose a new IoT architecture that utilizes the component-based design approach to create and define the loosely-coupled, standalone but interoperable service components for IoT systems. Furthermore, a data-driven feedback function is included as a key feature of the proposed architecture to enable a greater degree of system automation and to reduce the dependency on mankind for data analysis and decision-making. The proposed architecture aims to tackle device interoperability, system reusability and the lack of data-driven functionality issues. Using a real-world use case on a proof-of-concept prototype, we examined the viability and usability of the proposed architecture.
    MeSH terms: Automation; Feedback; Software; Internet
  7. Lee FH, Wan SY, Foo HL, Loh TC, Mohamad R, Abdul Rahim R, et al.
    Int J Mol Sci, 2019 Oct 09;20(20).
    PMID: 31600952 DOI: 10.3390/ijms20204979
    Biotransformation via solid state fermentation (SSF) mediated by microorganisms is a promising approach to produce useful products from agricultural biomass. Lactic acid bacteria (LAB) that are commonly found in fermented foods have been shown to exhibit extracellular proteolytic, β-glucosidase, β-mannosidase, and β-mannanase activities. Therefore, extracellular proteolytic, cellulolytic, and hemicellulolytic enzyme activities of seven Lactobacillus plantarum strains (a prominent species of LAB) isolated from Malaysian foods were compared in this study. The biotransformation of palm kernel cake (PKC) biomass mediated by selected L. plantarum strains was subsequently conducted. The results obtained in this study exhibited the studied L. plantarum strains produced versatile multi extracellular hydrolytic enzyme activities that were active from acidic to alkaline pH conditions. The highest total score of extracellular hydrolytic enzyme activities were recorded by L. plantarum RI11, L. plantarum RG11, and L. plantarum RG14. Therefore, they were selected for the subsequent biotransformation of PKC biomass via SSF. The hydrolytic enzyme activities of treated PKC extract were compared for each sampling interval. The scanning electron microscopy analyses revealed the formation of extracellular matrices around L. plantarum strains attached to the surface of PKC biomass during SSF, inferring that the investigated L. plantarum strains have the capability to grow on PKC biomass and perform synergistic secretions of various extracellular proteolytic, cellulolytic, and hemicellulolytic enzymes that were essential for the effective biodegradation of PKC. The substantial growth of selected L. plamtraum strains on PKC during SSF revealed the promising application of selected L. plantarum strains as a biotransformation agent for cellulosic biomass.
    MeSH terms: Biodegradation, Environmental; Biotransformation*; Cellulose/metabolism*; Cellulose/chemistry; Extracellular Space/metabolism; Fermentation; Food Microbiology*; Hydrolysis; Biomass*; Lactobacillales/isolation & purification; Lactobacillales/metabolism*; Proteolysis; Phoeniceae/chemistry*
  8. Mohd Hassan N, Yusof NA, Yahaya AF, Mohd Rozali NN, Othman R
    Antioxidants (Basel), 2019 Oct 09;8(10).
    PMID: 31600964 DOI: 10.3390/antiox8100469
    Pepper of the Capsicum species is a common ingredient in various food preparations by different cultures worldwide. The Capsicum is recognised by its five main domesticated species, namely Capsicum annuum, C. baccatum, C. chinense, C. frutescens and C. pubescens. The genetic diversity in Capsicum offers fruits in wide ranges of morphology and carotenoid profile. Carotenoids enhance the value of pepper from a nutritional standpoint, despite being commonly prized for the pharmacologically active pungent capsaicinoids. Carotenoids of pepper comprise mainly of the unique, powerful and highly stable capsanthin and capsoroubin, together with β-carotene, β-cryptoxanthin, lutein, zeaxanthin, antheraxanthin and violaxanthin. These carotenoids are present at diverse profile and varying levels, biosynthetically connected to the fruit maturity stages. This review describes the health-promoting functional attributes of the carotenoids that are mainly associated with their excellent role as lipophilic antioxidants. Capsicum as a great source of carotenoids is discussed in the aspects of main domesticated species, biosynthesis, pigment profile, antioxidant activity and safety. Findings from a number of in vitro, in vivo and clinical studies provided appreciable evidence on the protective effects of pepper's carotenoids against degenerative diseases. Hence, pepper with its functional carotenoids might be recommended in health-promoting and disease preventing strategies.
    MeSH terms: Antioxidants; Capsaicin; Capsicum; Carotenoids; Fruit; Pigmentation; Genetic Variation; Lutein; beta Carotene; Xanthophylls; Piper nigrum; Zeaxanthins; Beta-Cryptoxanthin
  9. Zafar R, Qayyum A, Mumtaz W
    J Integr Neurosci, 2019 Sep 30;18(3):217-229.
    PMID: 31601069 DOI: 10.31083/j.jin.2019.03.164
    In the electroencephalogram recorded data are often confounded with artifacts, especially in the case of eye blinks. Different methods for artifact detection and removal are discussed in the literature, including automatic detection and removal. Here, an automatic method of eye blink detection and correction is proposed where sparse coding is used for an electroencephalogram dataset. In this method, a hybrid dictionary based on a ridgelet transformation is used to capture prominent features by analyzing independent components extracted from a different number of electroencephalogram channels. In this study, the proposed method has been tested and validated with five different datasets for artifact detection and correction. Results show that the proposed technique is promising as it successfully extracted the exact locations of eye blinking artifacts. The accuracy of the method (automatic detection) is 89.6% which represents a better estimate than that obtained by an extreme machine learning classifier.
    MeSH terms: Machine Learning; Blinking; Electroencephalography; Artifacts
  10. Rajapakse S, de Silva NL, Weeratunga P, Rodrigo C, Sigera C, Fernando SD
    BMC Complement Altern Med, 2019 Oct 11;19(1):265.
    PMID: 31601215 DOI: 10.1186/s12906-019-2678-2
    BACKGROUND: Carica papaya (CP) extract is becoming popular as an unlicensed herbal remedy purported to hasten recovery in dengue infection, mostly based on observations that it may increase platelet counts. This systematic review and meta-analysis aims to critically analyze the evidence from controlled clinical trials on the efficacy and safety of CP extract in the treatment of dengue infection.

    METHODS: PubMed, LILACS and Google Scholar were searched for randomized or non-randomized trials enrolling patients with suspected or confirmed dengue where CP extract was compared, as a treatment measure, against standard treatment. Recovery of platelet counts as well as other clinical indicators of favourable outcome (duration of hospital stay, prevention of plasma leakage, life threatening complications, and mortality) were assessed.

    RESULTS: Nine studies (India-6, Pakistan-1, Indonesia-1, Malaysia-1) met the inclusion criteria. Seven studies showed an increase in platelet counts in patients receiving CP extract, while one study showed no significant difference between the two groups, and direct comparison was not possible in the remaining study. Serious adverse events were not reported. CP extract may reduce the duration of hospital stay (mean difference - 1.98 days, 95% confidence interval - 1.83 to - 2.12, 3 studies, 580 participants, low quality evidence), and cause improvement in mean platelet counts between the first and fifth day of treatment (mean difference 35.45, 95% confidence interval 23.74 to 47.15, 3 studies, 129 participants, low quality evidence). No evidence was available regarding other clinical outcomes.

    CONCLUSIONS: The clinical value of improvement in platelet count or early discharge is unclear in the absence of more robust indicators of favourable clinical outcome. Current evidence is insufficient to comment on the role of CP extract in dengue. There is a need for further well designed clinical trials examining the effect of CP on platelet counts, plasma leakage, other serious manifestations of dengue, and mortality, with clearly defined outcome measures.

    MeSH terms: Antiviral Agents/administration & dosage*; Antiviral Agents/chemistry; Clinical Trials as Topic; Dengue/drug therapy*; Dengue/virology; Humans; Plant Extracts/administration & dosage*; Plant Extracts/chemistry; Plant Leaves/chemistry; Carica/chemistry*
  11. Jazayeri SD, Poh CL
    Vet Res, 2019 Oct 10;50(1):78.
    PMID: 31601266 DOI: 10.1186/s13567-019-0698-z
    Veterinary vaccines need to have desired characteristics, such as being effective, inexpensive, easy to administer, suitable for mass vaccination and stable under field conditions. DNA vaccines have been proposed as potential solutions for poultry diseases since they are subunit vaccines with no risk of infection or reversion to virulence. DNA vaccines can be utilized for simultaneous immunizations against multiple pathogens and are relatively easy to design and inexpensive to manufacture and store. Administration of DNA vaccines has been shown to stimulate immune responses and provide protection from challenges in different animal models. Although DNA vaccines offer advantages, setbacks including the inability to induce strong immunity, and the fact that they are not currently applicable for mass vaccination impede the use of DNA vaccines in the poultry industry. The use of either biological or physical carriers has been proposed as a solution to overcome the current delivery limitations of DNA vaccines for veterinary applications. This review presents an overview of the recent development of carriers for delivery of veterinary DNA vaccines against avian pathogens.
    MeSH terms: Animals; Birds; Immunization; Poultry; Poultry Diseases; Virulence; Vaccines, DNA; Vaccines, Subunit; Models, Animal; Mass Vaccination
  12. Schepaschenko D, Chave J, Phillips OL, Lewis SL, Davies SJ, Réjou-Méchain M, et al.
    Sci Data, 2019 10 10;6(1):198.
    PMID: 31601817 DOI: 10.1038/s41597-019-0196-1
    Forest biomass is an essential indicator for monitoring the Earth's ecosystems and climate. It is a critical input to greenhouse gas accounting, estimation of carbon losses and forest degradation, assessment of renewable energy potential, and for developing climate change mitigation policies such as REDD+, among others. Wall-to-wall mapping of aboveground biomass (AGB) is now possible with satellite remote sensing (RS). However, RS methods require extant, up-to-date, reliable, representative and comparable in situ data for calibration and validation. Here, we present the Forest Observation System (FOS) initiative, an international cooperation to establish and maintain a global in situ forest biomass database. AGB and canopy height estimates with their associated uncertainties are derived at a 0.25 ha scale from field measurements made in permanent research plots across the world's forests. All plot estimates are geolocated and have a size that allows for direct comparison with many RS measurements. The FOS offers the potential to improve the accuracy of RS-based biomass products while developing new synergies between the RS and ground-based ecosystem research communities.
    MeSH terms: Conservation of Natural Resources; Environmental Monitoring/methods; Biomass*; Remote Sensing Technology*; Forests*
  13. Acquah C, Chan YW, Pan S, Yon LS, Ongkudon CM, Guo H, et al.
    Sci Rep, 2019 10 10;9(1):14501.
    PMID: 31601836 DOI: 10.1038/s41598-019-50862-1
    Immobilisation of aptameric ligands on solid stationary supports for effective binding of target molecules requires understanding of the relationship between aptamer-polymer interactions and the conditions governing the mass transfer of the binding process. Herein, key process parameters affecting the molecular anchoring of a thrombin-binding aptamer (TBA) onto polymethacrylate monolith pore surface, and the binding characteristics of the resulting macroporous aptasensor were investigated. Molecular dynamics (MD) simulations of the TBA-thrombin binding indicated enhanced Guanine 4 (G4) structural stability of TBA upon interaction with thrombin in an ionic environment. Fourier-transform infrared spectroscopy and thermogravimetric analyses were used to characterise the available functional groups and thermo-molecular stability of the immobilised polymer generated with Schiff-base activation and immobilisation scheme. The initial degradation temperature of the polymethacrylate stationary support increased with each step of the Schiff-base process: poly(Ethylene glycol Dimethacrylate-co-Glycidyl methacrylate) or poly(EDMA-co-GMA) [196.0 °C (±1.8)]; poly(EDMA-co-GMA)-Ethylenediamine [235.9 °C (±6.1)]; poly(EDMA-co-GMA)-Ethylenediamine-Glutaraldehyde [255.4 °C (±2.7)]; and aptamer-modified monolith [273.7 °C (±2.5)]. These initial temperature increments reflected in the associated endothermic energies were determined with differential scanning calorimetry. The aptameric ligand density obtained after immobilisation was 480 pmol/μL. Increase in pH and ionic concentration affected the surface charge distribution and the binding characteristics of the aptamer-modified disk-monoliths, resulting in the optimum binding pH and ionic concentration of 8.0 and 5 mM Mg2+, respectively. These results are critical in understanding and setting parametric constraints indispensable to develop and enhance the performance of aptasensors.
    MeSH terms: Calorimetry, Differential Scanning; Ethylenediamines; Glutaral; Guanine; Hydrogen-Ion Concentration; Ligands; Magnesium; Methacrylates; Polymers; Spectrum Analysis; Temperature; Thrombin; Molecular Dynamics Simulation
  14. Bhuvanendran S, Bakar SNS, Kumari Y, Othman I, Shaikh MF, Hassan Z
    Sci Rep, 2019 10 10;9(1):14507.
    PMID: 31601902 DOI: 10.1038/s41598-019-50954-y
    Alzheimer's disease (AD) is the second most occurring neurological disorder after stroke and is associated with cerebral hypoperfusion, possibly contributing to cognitive impairment. In the present study, neuroprotective and anti-AD effects of embelin were evaluated in chronic cerebral hypoperfusion (CCH) rat model using permanent bilateral common carotid artery occlusion (BCCAO) method. Rats were administered with embelin at doses of 0.3, 0.6 or 1.2 mg/kg (i.p) on day 14 post-surgery and tested in Morris water maze (MWM) followed by electrophysiological recordings to access cognitive abilities and synaptic plasticity. The hippocampal brain regions were extracted for gene expression and neurotransmitters analysis. Treatment with embelin at the doses of 0.3 and 0.6 mg/kg significantly reversed the spatial memory impairment induced by CCH in rats. Embelin treatment has significantly protected synaptic plasticity impairment as assessed by hippocampal long-term potentiation (LTP) test. The mechanism of this study demonstrated that embelin treatment alleviated the decreased expression of BDNF, CREB1, APP, Mapt, SOD1 and NFκB mRNA levels caused by CCH rats. Furthermore, treatment with embelin demonstrated neuromodulatory activity by its ability to restore hippocampal neurotransmitters. Overall these data suggest that embelin improve memory and synaptic plasticity impairment in CCH rats and can be a potential drug candidate for neurodegenerative disease-related cognitive disorders.
    MeSH terms: Alzheimer Disease/drug therapy*; Alzheimer Disease/genetics; Alzheimer Disease/pathology; Animals; Brain Ischemia/drug therapy*; Brain Ischemia/genetics; Brain Ischemia/pathology; Gene Expression Regulation/drug effects; Hippocampus/metabolism; Hippocampus/pathology; Humans; Neurons/drug effects; RNA, Messenger/genetics; Benzoquinones/pharmacology*; Amyloid beta-Protein Precursor/genetics; tau Proteins/genetics; Cyclic AMP Response Element-Binding Protein/genetics; Long-Term Potentiation/drug effects; Neuroprotective Agents/pharmacology*; Maze Learning; Brain-Derived Neurotrophic Factor/genetics; Rats; Mild Cognitive Impairment/drug therapy*; Mild Cognitive Impairment/genetics; Mild Cognitive Impairment/pathology; Mild Cognitive Impairment/prevention & control; Spatial Memory/drug effects; Superoxide Dismutase-1/genetics
  15. Hakim HM, Khan HO, Ismail SA, Ayob S, Lalung J, Kofi EA, et al.
    Sci Rep, 2019 10 10;9(1):14558.
    PMID: 31601905 DOI: 10.1038/s41598-019-51154-4
    Short repetitive regions in autosomal and Y chromosomes known as short tandem repeats (STRs) are currently used for DNA profiling in crime investigations. However, DNA profiling requires a sufficient quality and quantity of DNA template, which is often not obtained from trace evidence or degraded biological samples collected at the scene of a crime. Here, we assessed autosomal and male DNA components extracted from crime scene and mock casework samples using the Casework Direct Kit, Custom and compared the results against those obtained by extraction of matching samples using well-established Maxwell 16 System DNA IQ Casework Pro Kit. The quantity and quality of extracted DNA obtained using both Casework Direct Kit, Custom and Maxwell 16 System DNA IQ Casework Pro Kit were analyzed using PowerQuant Systems followed by autosomal and Y-chromosome STR profiling using GlobalFiler Express PCR Amplification Kit and PowerPlex Y23 System, respectively. Our results showed that the Casework Direct Kit and Maxwell 16 DNA IQ Casework Pro Kit have more or less equal capacity to extract inhibitor free DNA, but that the latter produces slightly better quality and more DNA template and subsequently higher numbers of STR allele calls for autosomal and Y-STR analyses. Nonetheless, the Casework Direct Kit, Custom is the quicker and cheaper option for extraction of good, clean DNA from high content material and might best be used for extraction of reference samples. Such reference DNA samples typically come from buccal swabs or freshly drawn blood. So, in general, they can confidently be expected to have a high nucleic acid content and to be inhibitor-free.
    MeSH terms: Alleles; Chromosomes; Crime; DNA/analysis*; Female; Humans; Male; Reference Values; Polymerase Chain Reaction; DNA Fingerprinting*; Microsatellite Repeats*; Chromosomes, Human, Y*; Forensic Genetics/instrumentation*
  16. Yoon C, Nam KC, Lee YK, Kang Y, Choi SJ, Shin HM, et al.
    J Korean Med Sci, 2019 Oct 14;34(39):e255.
    PMID: 31602825 DOI: 10.3346/jkms.2019.34.e255
    BACKGROUND: Medical device adverse event reporting is an essential activity for mitigating device-related risks. Reporting of adverse events can be done by anyone like healthcare workers, patients, and others. However, for an individual to determine the reporting, he or she should recognize the current situation as an adverse event. The objective of this report is to share observed individual differences in the perception of a medical device adverse event, which may affect the judgment and the reporting of adverse events.

    METHODS: We trained twenty-three participants from twelve Asia-Pacific Economic Cooperation (APEC) member economies about international guidelines for medical device vigilance. We developed and used six virtual cases and six questions. We divided participants into six groups and compared their opinions. We also surveyed the country's opinion to investigate the beginning point of 'patient use'. The phases of 'patient use' are divided into: 1) inspecting, 2) preparing, and 3) applying medical device.

    RESULTS: As for the question on the beginning point of 'patient use,' 28.6%, 35.7%, and 35.7% of participants provided answers regarding the first, second, and third phases, respectively. In training for applying international guidelines to virtual cases, only one of the six questions reached a consensus between the two groups in all six virtual cases. For the other five questions, different judgments were given in at least two groups.

    CONCLUSION: From training courses using virtual cases, we found that there was no consensus on 'patient use' point of view of medical devices. There was a significant difference in applying definitions of adverse events written in guidelines regarding the medical device associated incidents. Our results point out that international harmonization effort is needed not only to harmonize differences in regulations between countries but also to overcome diversity in perspectives existing at the site of medical device use.

    MeSH terms: Adult; Contact Lenses/adverse effects; Corneal Ulcer/etiology; Female; Foreign Bodies/etiology; Health Personnel/education; Health Personnel/psychology*; Humans; Male; Middle Aged; Program Evaluation*; Stents/adverse effects; Guidelines as Topic; Medical Errors*
  17. Sidra S, Tariq MH, Farrukh MJ, Mohsin M
    PLoS One, 2019;14(10):e0223329.
    PMID: 31603907 DOI: 10.1371/journal.pone.0223329
    This study aimed to evaluate the clinical manifestations and health risks associated with polycystic ovary syndrome (PCOS) and its impact on quality of life (QOL) in Pakistan. A detailed cross-sectional study was conducted on PCOS among women of reproductive age visiting the gynecology and obstetrics and endocrinology departments at primary and tertiary care hospitals located in Abbottabad, Kohat, and Islamabad. In total, 440 patients meeting the inclusion criteria were included. A checklist was specifically designed to identify symptoms and health risks, including adverse drug reactions, complications, irrational prescription or underprescription, and drug-drug interactions. The Short Form-12 questionnaire was used to evaluate the QOL of patients with PCOS. Data collected were analyzed for descriptive and inferential statistics using chi-square test, analysis of variance, and post hoc analysis. All patients exhibited the cardinal symptoms of PCOS, including obesity (n = 352, 80%), acne (n = 296, 67.3), hirsutism (n = 299, 68%), hyperglycemia (n = 278, 63.2%), and irregular menstruation (n = 316, 71.8%). Ultrasonography confirmed that 268 (61%) patients had multiple cysts of >10 mm in diameter. Patients with untreated PCOS exhibited a high prevalence of health risks including hypertension (n = 87, 19.8%), diabetes (n = 268, 60.9%), sleep apnea (n = 11, 2.5%), infertility (n = 146, 33.2%), increased endometrial thickness (n = 21, 4.8%), miscarriages (n = 68, 15.5%), high cholesterol level (n = 85, 19.3%), and hyperandrogenism (n = 342, 77.7%). Most patients exhibited low QOL scores (n = 374, 85%), with depression being the largest contributor to low QOL. Apart from novel results, this study found an association between depression and low QOL in patients with PCOS, suggesting the need for reviewing the management guidelines and psychological health assessment of women with PCOS.
    MeSH terms: Adolescent; Adult; Analysis of Variance; Female; Humans; Polycystic Ovary Syndrome/complications; Polycystic Ovary Syndrome/pathology*; Drug Prescriptions; Quality of Life*; Risk Factors; Young Adult; Drug-Related Side Effects and Adverse Reactions/etiology
  18. Kar SS, Bhat VG, Shenoy VP, Bairy I, Shenoy GG
    Chem Biol Drug Des, 2019 01;93(1):60-66.
    PMID: 30118192 DOI: 10.1111/cbdd.13379
    In our efforts to develop druggable diphenyl ethers as potential antitubercular agents, a series of novel diphenyl ether derivatives (5a-f, 6a-f) were designed and synthesized. The representative compounds showed promising in vitro activity against drug-susceptible, isoniazid-resistant, and multidrug-resistant strains of Mycobacterium tuberculosis with MIC values of 1.56 μg/ml (6b), 6.25 μg/ml (6a-d), and 3.125 μg/ml (6b-c), respectively. All the synthesized compounds exhibited satisfactory safety profile (CC50  > 300 μg/ml) against Vero and HepG2 cells. Reverse phase HPLC method was used to probe the physicochemical properties of the synthesized compounds. This series of compounds demonstrated comparatively low logP values. pKa values of representative compounds indicated that they were weak acids. Additionally, in vitro human liver microsomal stability assay confirmed that the synthesized compounds possessed acceptable stability under study conditions. The present study thus establishes compound 6b as the most promising antitubercular agent with acceptable drug-likeness.
    MeSH terms: Antitubercular Agents; Biochemical Phenomena; Chromatography, High Pressure Liquid; Humans; Isoniazid; Liver; Microbial Sensitivity Tests; Mycobacterium tuberculosis; Phenyl Ethers; Hep G2 Cells
  19. Lim EWL, Chee ML, Sabanayagam C, Majithia S, Tao Y, Wong TY, et al.
    Invest Ophthalmol Vis Sci, 2019 05 01;60(6):1889-1897.
    PMID: 31042796 DOI: 10.1167/iovs.19-26810
    Purpose: The purpose of this study was to investigate the association between sleep (duration and quality) and symptoms of dry eye in Singapore Malay and Indian adults.

    Methods: This was a prospective cross-sectional study. A total of 3303 subjects aged 40 years and above from two large population-based cohorts, the Singapore Malay Eye Study-2 (n = 1191, 2011-2013) and the Singapore Indian Eye Study-2 (n = 2112, 2013-2015), were included. The presence of symptoms of dry eye was defined as having at least one of six symptoms often or all the time. Sleep questionnaires included the Epworth Sleepiness Scale, Berlin Questionnaire, STOP-bang questionnaire, and Insomnia Severity Index. Poor sleep quality was defined as meeting the respective questionnaire thresholds. General health questionnaires (including sleep duration) and standardized ocular and systemic tests were also used.

    Results: Of 3303 participants, 6.4% had excessive sleepiness, 20.5% had high risk for sleep apnea, 2.7% had clinical insomnia, and 7.8% had <5 hours of sleep. These sleep factors were associated with symptoms of dry eye. After adjusting for relevant demographic, medical, and social factors, the following were associated with higher odds of symptoms of dry eye: excessive sleepiness (Epworth Sleepiness Scale: odds ratio [OR] = 1.77 [1.15-2.71]), high risk of sleep apnea (Berlin Questionnaire: OR = 1.55 [1.17-2.07], STOP-Bang Questionnaire: OR = 2.66 [1.53-4.61]), clinical insomnia (Insomnia Severity Index: OR = 3.68 [2.17-6.26]) and <5 hours of sleep (OR = 1.73 [1.17-2.57], reference sleep duration 5-9 hours). Sleep apnea, insomnia, and sleep duration were each shown to be independently associated with symptoms of dry eye.

    Conclusion: Short sleep duration and poor quality are both significantly and independently associated with symptoms of dry eye.

    MeSH terms: Aged; Aged, 80 and over; Cross-Sectional Studies; Female; Follow-Up Studies; Humans; India/ethnology; Malaysia/ethnology; Male; Middle Aged; Prospective Studies; Singapore/epidemiology; Sleep/physiology*; Tears/metabolism*; Time Factors; Dry Eye Syndromes/ethnology; Dry Eye Syndromes/etiology; Dry Eye Syndromes/metabolism*; Incidence; Polysomnography; Sleep Apnea, Obstructive/ethnology; Sleep Apnea, Obstructive/metabolism*; Sleep Apnea, Obstructive/physiopathology
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