Browse publications by year: 2019

  1. Seaman DJI, Bernard H, Ancrenaz M, Coomes D, Swinfield T, Milodowski DT, et al.
    Am J Primatol, 2019 08;81(8):e23030.
    PMID: 31328289 DOI: 10.1002/ajp.23030
    The conversion of forest to agriculture continues to contribute to the loss and fragmentation of remaining orang-utan habitat. There are still few published estimates of orang-utan densities in these heavily modified agricultural areas to inform range-wide population assessments and conservation strategies. In addition, little is known about what landscape features promote orang-utan habitat use. Using indirect nest count methods, we implemented surveys and estimated population densities of the Northeast Bornean orang-utan (Pongo pygmaeus morio) across the continuous logged forest and forest remnants in a recently salvage-logged area and oil palm plantations in Sabah, Malaysian Borneo. We then assessed the influence of landscape features and forest structural metrics obtained from LiDAR data on estimates of orang-utan density. Recent salvage logging appeared to have a little short-term effect on orang-utan density (2.35 ind/km 2 ), which remained similar to recovering logged forest nearby (2.32 ind/km 2 ). Orang-utans were also present in remnant forest patches in oil palm plantations, but at significantly lower numbers (0.82 ind/km 2 ) than nearby logged forest and salvage-logged areas. Densities were strongly influenced by variation in canopy height but were not associated with other potential covariates. Our findings suggest that orang-utans currently exist, at least in the short-term, within human-modified landscapes, providing that remnant forest patches remain. We urge greater recognition of the role that these degraded habitats can have in supporting orang-utan populations, and that future range-wide analyses and conservation strategies better incorporate data from human-modified landscapes.
    MeSH terms: Animals; Conservation of Natural Resources; Malaysia; Pongo pygmaeus*; Population Density; Ecosystem*; Arecaceae; Forests*
  2. Chan CMH, Ab Rahman J, Tee GH, Wee LH, Ho BK, Robson NZMH, et al.
    Asia Pac J Public Health, 2019 10;31(7_suppl):32S-43S.
    PMID: 31328531 DOI: 10.1177/1010539519860730
    Little is known about the perceptions of harm and benefit associated with the use of e-cigarettes in Malaysia. This was a cross-sectional analysis of a nationally representative sample comprising 1987 males (≥18 years of age). Current, former, and never users of conventional cigarettes and/or e-cigarettes participated in a questionnaire study conducted via face-to-face interviews. The relationship between participant characteristics and perceptions of harm and benefit of e-cigarettes were determined with multivariable logistic regression. There were 950 current, 377 former, and 660 never users of e-cigarettes. Government employees (odds ratio [OR] = 1.85, 95% confidence interval [CI] = 1.29-2.66, P = .001), private sector employees (OR = 1.67, 95% CI = 1.27-2.18, P = .001), and the self-employed people (OR = 1.68, 95% CI = 1.31-2.17, P = .001) were more likely to perceive e-cigarettes as more harmful than conventional cigarettes compared with respondents who were not wage earners. All current users in the form of e-cigarette users (OR = 7.87, 95% CI = 3.23-19.18), conventional cigarette smokers (OR = 1.80, 95% CI = 1.39-2.33), and dual users (OR = 8.59, 95% CI = 4.76-15.52) were more likely to perceive e-cigarettes as useful in quitting conventional cigarette smoking compared with former and never users. Our findings constitute an important snapshot into the perceptions of e-cigarette harms and benefits, which could inform targeted public health messaging strategies.
    MeSH terms: Adolescent; Adult; Aged; Cross-Sectional Studies; Humans; Health Knowledge, Attitudes, Practice*; Malaysia/epidemiology; Male; Middle Aged; Surveys and Questionnaires; Smoking/epidemiology; Risk Assessment; Young Adult; Electronic Cigarettes*; Vaping/epidemiology
  3. Li L, Zhang W, Desikan Seshadri VD, Cao G
    Artif Cells Nanomed Biotechnol, 2019 Dec;47(1):3029-3036.
    PMID: 31328556 DOI: 10.1080/21691401.2019.1642902
    Nowadays, the synthesis and characterization of gold nanoparticles (AuNPs) from plant based extracts and effects of their anticancer have concerned an important interest. Marsdenia tenacissima (MT), a conventional Chinese herbal medicine, has long been used for thousands of years to treat tracheitis, asthma, rheumatism, etc. In this present study, we optimize the reaction of parameters to manage the nanoparticle size, which was categorized by high-resolution transmission electron microscopy (HR-TEM). A different characterization method, for example, UV-visible spectroscopy (UV-vis), fourier-transform infrared spectroscopy (FT-IR) and X-ray diffraction (XRD) were performed to consider the synthesized AuNPs getting from the MT leaf extract. The MT-AuNPs were analyzed for their cytotoxicity property against HepG2 cells by MTT analysis. The apoptosis was evaluated by using reactive oxygen species (ROS), migration assay, mitochondrial membrane potential (MMP) and apoptotic protein expression. Interestingly, the findings of our study observed the cytotoxicity effect of synthesized MT-AuNPs at a concentration of 59.62 ± 4.37 μg after 24 hrs treatment. Apoptosis was induced by the MT-AuNPs with enhanced ROS, changed MMP and inhibit the migration assay. Finally, the apoptosis was confirmed by the considerable up-regulation of Bax, caspase-9 and caspase-3, while the anti-apoptotic protein expressions of Bcl-2 and Bcl-XL were down-regulated. Although, in this studies, we evaluated the characterization, synthesis and anticancer action of gold nanoparticles from MT (MT-AuNPS) helpful for liver cancer therapeutics.
    MeSH terms: Antineoplastic Agents/chemical synthesis; Antineoplastic Agents/pharmacology*; Antineoplastic Agents/chemistry*; Gold/pharmacology*; Gold/chemistry*; Humans; Liver Neoplasms/pathology*; Plant Extracts/chemistry; Marsdenia/chemistry*; Nanotechnology; Membrane Potential, Mitochondrial/drug effects; Metal Nanoparticles/chemistry*; Hep G2 Cells; Chemistry Techniques, Synthetic
  4. Yellappan K, K C Mani K, Md Tamrin SB
    Traffic Inj Prev, 2019;20(6):624-629.
    PMID: 31329467 DOI: 10.1080/15389588.2019.1626985
    Objective: The objective of this study was to determine the proportion of motorcycle safety helmets (MSHs) used by postal delivery riders (PDRs) that comply with the Standards and Industrial Research Institute of Malaysia's (SIRIM) MSH standard guidelines and identify factors that contribute toward compliance of used MSHs with the standards. Methods: The presence of SIRIM certification label, the status of MSH, type of chinstrap, MSH crash history, and duration of MSH use were observed. The dependent variable was the results of the SIRIM testing procedures (SIRIM tests). MSHs that passed the SIRIM tests were considered "standard certified" MSHs. Results: The odds of the complimentary MSHs passing all of the SIRIM tests were 3.7 times the odds of the self-purchased MSHs passing the tests. The odds of MSHs with the SIRIM certification label passing all of the SIRIM tests were 24.2 times the odds of MSHs without the SIRIM certification label, and the odds of MSHs used <3 years passing the SIRIM tests were 3.75 times the odds of the MSHs used ≥3.8 years. Conclusion: PDRs provided with complimentary MSHs with the SIRIM certification label by the employer for their daily delivery routines and duration of MSH used for less than 3 years were found to be safe MSHs for male occupational riders in Malaysia.
    MeSH terms: Accidents, Traffic/statistics & numerical data; Certification; Cross-Sectional Studies; Head Protective Devices/standards*; Humans; Malaysia; Male; Motorcycles*; Postal Service; Surveys and Questionnaires
  5. Bohari A, Hashim S, Ghoshal SK, Mohd Mustafa SN
    Radiat Prot Dosimetry, 2019 Dec 31;186(4):462-468.
    PMID: 31329977 DOI: 10.1093/rpd/ncz051
    Long exposure to radiation from fluoroscopy-guided interventions (FGIs) can be detrimental to both patients and radiologists. The effective doses received by the interventional radiology staff after performing 230 FGIs in a year were assessed by using double dosimetry and five various algorithms. The Shapiro-Wilk test revealed normally-distributed data (p < 0.01), while the significant correlation coefficients between the effective doses ranged between 0.88 and 1.00. As for the Bland-Altman analysis, both Niklason and Boetticher algorithms strongly supported the absence of statistical significance between the estimated effective doses. This portrays that the occupational doses received by the interventional radiology staff during FGIs fall within the acceptable limit regardless of the varied algorithms applied. In short, the Niklason and Boetticher algorithms appeared to be the more interchangeable ones for effective evaluation of doses. This is in view of their strong mutual correlations and excellent agreement.
    MeSH terms: Algorithms*; Fluoroscopy/methods*; Humans; Radiation Dosage; Radiation Injuries/prevention & control*; Radiation Protection/methods*; Thermoluminescent Dosimetry/methods*; Radiology, Interventional/methods*; Occupational Exposure/analysis*; Radiologists
  6. Gujjar KR, van Wijk A, Kumar R, de Jongh A
    J Evid Based Dent Pract, 2019 06;19(2):140-155.
    PMID: 31326046 DOI: 10.1016/j.jebdp.2019.01.009
    OBJECTIVES: The aim of this study was to evaluate the effectiveness of technology-based interventions for the treatment of dental anxiety in children and adults.

    DATA SOURCES: A systematic search using relevant keywords was conducted in PubMed-Medline, EMBASE, PsycINFO, CINAHL, Scopus, and The Cochrane Library.

    INCLUSION CRITERIA: Randomized controlled trials (RCTs) that compared technology-based interventions with inactive controls in the treatment of moderate to severe dental anxiety were included.

    RESULTS: A total of seven RCTs were included in the review. These studies investigated the effectiveness of video modeling, computerized cognitive behavioral therapy, virtual reality exposure therapy, and distraction with music and audiovisual video material. Six studies examining video modeling, computerized cognitive behavioral therapy, virtual reality exposure therapy, and distraction (audiovisual) showed significantly greater reductions in dental anxiety than inactive controls in both children and adults. None of the included studies followed Consolidated Standards of Reporting Trials guidelines completely or reported sufficient data, thereby precluding a possible meta-analysis. Four out of seven included studies were assessed to be at high risk of bias.

    CONCLUSIONS: A limited number of studies supported the effectiveness of technology-based interventions in the treatment of dental anxiety in children and adults.

    CLINICAL SIGNIFICANCE: The quality of the methods of studies on the effects of technology-based interventions allows only limited inferences on the effects of these interventions. However, within the limitations of the systematic review, the results converge to suggest that technology-based interventions may be useful as an adjunct to standard dental care. High-quality RCTs are needed to determine the (relative) effectiveness of these interventions.

    PROSPERO REGISTRATION NUMBER: CRD42017064810.

    MeSH terms: Adult; Child; Humans; Implosive Therapy*; Cognitive Therapy*; Dental Anxiety*
  7. Yahya N, Manan HA
    World Neurosurg, 2019 Oct;130:e188-e198.
    PMID: 31326352 DOI: 10.1016/j.wneu.2019.06.027
    BACKGROUND: Diffusion tensor imaging (DTI), which visualizes white matter tracts, can be integrated to optimize intracranial radiation therapy (RT) and radiosurgery (RS) treatment planning. This study aimed to systematically review the integration of DTI for dose optimization in terms of evidence of dose improvement, clinical parameter changes, and clinical outcome in RT/RS treatment planning.

    METHODS: PubMed and Scopus electronic databases were searched based on the guidelines established by PRISMA to obtain studies investigating the integration of DTI in intracranial RT/RS treatment planning. References and citations from Google Scholar were also extracted. Eligible studies were extracted for information on changes in dose distribution, treatment parameters, and outcome after DTI integration.

    RESULTS: Eighteen studies were selected for inclusion with 406 patients (median study size, 19; range: 2-144). Dose distribution, with or without DTI integration, described changes of treatment parameters, and the reported outcome of treatment were compared in 12, 7, and 10 studies, respectively. Dose distributions after DTI integration improved in all studies. Delivery time or monitor unit was higher after integration. In studies with long-term follow-up (median, >12 months), neurologic deficits were significantly fewer in patients with DTI integration.

    CONCLUSIONS: Integrating DTI into RT/RS treatment planning improved dose distribution, with higher treatment delivery time or monitor unit as a potential drawback. Fewer neurologic deficits were found with DTI integration.

    MeSH terms: Brain Neoplasms/radiotherapy; Humans; Prospective Studies; Radiation Dosage*; Radiotherapy/methods*; Radiosurgery/methods*; Diffusion Tensor Imaging/methods*
  8. Abd Rahim MH, Lim EJ, Hasan H, Abbas A
    J Microbiol Methods, 2019 09;164:105672.
    PMID: 31326443 DOI: 10.1016/j.mimet.2019.105672
    PURPOSE: This study aimed to assess the effect of nitrogen, salt and pre-culture conditions on the production of lovastatin in A. terreus ATCC 20542.

    METHODS: Different combinations of nitrogen sources, salts and pre-culture combinations were applied in the fermentation media and lovastatin yield was analysed chromatographically.

    RESULT: The exclusion of MnSO4 ·5H2O, CuSO4·5H2O and FeCl3·6H2O were shown to significantly improve lovastatin production (282%), while KH2PO4, MgSO4·7H2O, and NaCl and ZnSO4·7H2O were indispensable for good lovastatin production. Simple nitrogen source (ammonia) was unfavourable for morphology, growth and lovastatin production. In contrast, yeast extract (complex nitrogen source) produced the highest lovastatin yield (25.52 mg/L), while powdered soybean favoured the production of co-metabolites ((+)-geodin and sulochrin). Intermediate lactose: yeast extract (5:4) ratio produced the optimal lovastatin yield (12.33 mg/L) during pre-culture, while high (5:2) or low (5:6) lactose to yeast extract ratio produced significantly lower lovastatin yield (7.98 mg/L and 9.12 mg/L, respectively). High spore concentration, up to 107 spores/L was shown to be beneficial for lovastatin, but not for co-metabolite production, while higher spore age was shown to be beneficial for all of its metabolites.

    CONCLUSION: The findings from these investigations could be used for future cultivation of A. terreus in the production of desired metabolites.

    MeSH terms: Aspergillus/metabolism*; Benzoates; Benzofurans; Culture Media/chemistry*; Fermentation; Lactose/metabolism; Lovastatin/biosynthesis*; Microbiological Techniques/methods*; Nitrogen; Spores/growth & development; Biomass; Cell Culture Techniques/methods; Ammonium Compounds
  9. Ishaqui AA, Khan AH, Syed Sulaiman SA, Alsultan MT, Khan I, Al Nami H
    Pak J Pharm Sci, 2019 May;32(3 (Supplementary)):1225-1233.
    PMID: 31326884
    The aim of the study is to assess and compare the impact of antiviral drug alone and in combination with antibiotic for prevention of Influenza-A H1N1 induced acute kidney injury (AKI) in hospitalized patients. Hospitalized admitted patients with confirmed diagnosis of Influenza-A H1N1 infection were divided into two groups: group 1, which received antiviral (oseltamivir) drug alone and group 2, which received antiviral (oseltamivir) in combination with empirically prescribed antibiotic. Patients of both groups were assessed for incidences of AKI by two criteria i.e Acute Kidney Injury Network (AKIN) and RIFLE. A total of 329 patients (176 for group 1 and 153 for group 2) were enrolled. According to RIFLE criteria, 23(13%) of group 1 and 9(6%) patients of groups 2 were suffered from AKI with statistically significant difference (P<0.05). Also as per AKIN criteria, the incidence of AKI is statistically significantly difference (P<0.05) between both groups with 18(10%) patients and 6(4%) patients of group 1 and 2 respectively. Length of hospitalization was statistically less (P<0.05) in group 2 patients. The incidences of AKI in Influenza-A H1N1 treated with antiviral and antibiotic combination was statistically less as compared to patients who were given antiviral alone for treatment of influenza infection.
    MeSH terms: Anti-Bacterial Agents; Antiviral Agents; Hospitalization; Humans; Influenza, Human; Incidence; Influenza A Virus, H1N1 Subtype; Oseltamivir; Acute Kidney Injury
  10. Mat-Shayuti MS, Tuan Ya TMYS, Abdullah MZ, Megat Khamaruddin PNF, Othman NH
    Environ Sci Pollut Res Int, 2019 Sep;26(26):26419-26438.
    PMID: 31327143 DOI: 10.1007/s11356-019-05954-w
    Steady efforts in using ultrasonic energy to treat oil-contaminated sand started in the early 2000s until today, although pilot studies on the area can be traced to even earlier dates. Owing to the unique characteristics of the acoustic means, the separation of oil from sand has been showing good results in laboratories. This review provides the compilation of researches and insights into the mechanism of separation thus far. Related topics in the areas of oil-contaminated sand characterizations, fundamental ultrasonic cleaning, and cavitation effects are also addressed. Nevertheless, many of the documented works are only at laboratory or pilot-scale level, and the comprehensive interaction between ultrasonic parameters towards cleaning efficiencies may not have been fully unveiled. Gaps and opportunities are also presented at the end of this article.
    MeSH terms: Ultrasonic Waves*; Acoustics; Petroleum/analysis*; Silicon Dioxide/chemistry*; Soil Pollutants/analysis*; Environmental Restoration and Remediation/methods*
  11. Rashid U, Ahmad J, Ibrahim ML, Nisar J, Hanif MA, Shean TYC
    Materials (Basel), 2019 Jul 18;12(14).
    PMID: 31323732 DOI: 10.3390/ma12142293
    The main purpose of this manuscript is to report the new usage of tea waste (TW) as a catalyst for efficient conversion of palm fatty acid distillate (PFAD) to biodiesel. In this work, we investigate the potential of tea waste char as a catalyst for biodiesel production before and after sulfonation. The activated sulfonated tea waste char catalyst was characterized using Fourier transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA), X-ray diffractometry (XRD), elemental composition (CHNS), nitrogen adsorption-desorption using Brunauer-Emmett-Teller (BET) and ammonia-temperature-programmed desorption (NH3-TPD). The activated tea waste char catalyst shows higher acid density of 31 μmol g-1 as compared to tea waste char of 16 μmol g-1 and higher surface area of 122 m2/g. The optimum fatty acid conversion conditions were found that 4 wt % of catalyst loading with 9:1 of methanol:PFAD for 90 min of reaction time at 65 °C gives 97% free fatty acid (FFA) conversion. In conclusion, the sulfonated tea waste (STW) catalyst showed an impressive catalytic activity towards the esterification of PFAD at optimum reaction conditions with significant recyclability in five successive cycles without any reactivation step.
  12. Lee HK, Talib ZA, Mamat Mat Nazira MS, Wang E, Lim HN, Mahdi MA, et al.
    Materials (Basel), 2019 Jul 18;12(14).
    PMID: 31323741 DOI: 10.3390/ma12142295
    The effect of NaOH solution on the formation of nanoparticles has been the subject of ongoing debate in selenium-based material research. In this project, the robust correlation between the mechanistic growth of zinc selenide/graphene oxide (ZnSe/GO) composite and the concentration of NaOH are elucidated. The ZnSe/GO composite was synthesized via microwave-assisted hydrothermal method and the concentrations of NaOH are controlled at 2 M, 3 M, 4 M, 5 M and 6 M. The XRD spectra show that the crystal phases of the samples exhibited a 100% purity of ZnSe when the concentration of sodium hydroxide (NaOH) was set at 4 M. The further increase of NaOH concentration leads to the formation of impurities. This result reflects the essential role of hydroxyl ions in modifying the purity state of ZnSe/GO composite. The optical band gap energy of ZnSe/GO composite also decreased from 2.68 eV to 2.64 eV when the concentration of NaOH increased from 2 M to 4 M. Therefore, it can be concluded that the optimum concentration of NaOH used in synthesizing ZnSe/GO composite is 4 M. This project provides an alternative green method in the formation of a high purity ZnSe/GO composite.
  13. Oladosu Y, Rafii MY, Samuel C, Fatai A, Magaji U, Kareem I, et al.
    Int J Mol Sci, 2019 Jul 18;20(14).
    PMID: 31323764 DOI: 10.3390/ijms20143519
    Drought is the leading threat to agricultural food production, especially in the cultivation of rice, a semi-aquatic plant. Drought tolerance is a complex quantitative trait with a complicated phenotype that affects different developmental stages in plants. The level of susceptibility or tolerance of rice to several drought conditions is coordinated by the action of different drought-responsive genes in relation with other stress components which stimulate signal transduction pathways. Interdisciplinary researchers have broken the complex mechanism of plant tolerance using various methods such as genetic engineering or marker-assisted selection to develop a new cultivar with improved drought resistance. The main objectives of this review were to highlight the current method of developing a durable drought-resistant rice variety through conventional breeding and the use of biotechnological tools and to comprehensively review the available information on drought-resistant genes, QTL analysis, gene transformation and marker-assisted selection. The response, indicators, causes, and adaptation processes to the drought stress were discussed in the review. Overall, this review provides a systemic glimpse of breeding methods from conventional to the latest innovation in molecular development of drought-tolerant rice variety. This information could serve as guidance for researchers and rice breeders.
    MeSH terms: Adaptation, Physiological/genetics; Adaptation, Physiological/physiology; Breeding*; Oryza/genetics; Oryza/metabolism; Oryza/physiology*; Stress, Physiological/genetics; Stress, Physiological/physiology; DNA Shuffling; Quantitative Trait Loci/genetics; Droughts
  14. Nurfarahin AH, Mohamed MS, Phang LY
    Molecules, 2019 Jul 18;24(14).
    PMID: 31323769 DOI: 10.3390/molecules24142613
    High production costs of biosurfactants are mainly caused by the usage of the expensive substrate and long fermentation period which undermines their potential in bioremediation processes, food, and cosmetic industries even though they, owing to the biodegradability, lower toxicity, and raise specificity traits. One way to circumvent this is to improvise the formulation of biosurfactant-production medium by using cheaper substrate. A culture medium utilizing palm fatty acid distillate (PFAD), a palm oil refinery by-product, was first developed through one-factor-at-a-time (OFAT) technique and further refined by means of the statistical design method of factorial and response surface modeling to enhance the biosurfactant production from Pseudomonas sp. LM19. The results shows that, the optimized culture medium containing: 1.148% (v/v) PFAD; 4.054 g/L KH2PO4; 1.30 g/L yeast extract; 0.023 g/L sodium-EDTA; 1.057 g/L MgSO4·7H2O; 0.75 g/L K2HPO4; 0.20 g/L CaCl2·2H2O; 0.080 g/L FeCl3·6H2O gave the maximum biosurfactant productivity. This study demonstrated that the cell concentration and biosurfactant productivity could reach up to 8.5 × 109 CFU/mL and 0.346 g/L/day, respectively after seven days of growth, which were comparable to the values predicted by an RSM regression model, i.e., 8.4 × 109 CFU/mL and 0.347 g/L/day, respectively. Eleven rhamnolipid congeners were detected, in which dirhamnolipid accounted for 58% and monorhamnolipid was 42%. All in all, manipulation of palm oil by-products proved to be a feasible substrate for increasing the biosurfactant production about 3.55-fold as shown in this study.
    MeSH terms: Biodegradation, Environmental; Culture Media*; Fatty Acids/chemistry*; Fermentation; Nitrogen/metabolism; Pseudomonas/metabolism*; Surface-Active Agents/analysis; Surface-Active Agents/metabolism*; Distillation
  15. Hisamuddin N, Shaik Mossadeq WM, Sulaiman MR, Abas F, Leong SW, Kamarudin N, et al.
    Molecules, 2019 Jul 18;24(14).
    PMID: 31323775 DOI: 10.3390/molecules24142614
    Curcumin, derived from the rhizome Curcuma longa, has been scientifically proven to possess anti-inflammatory activity but is of limited clinical and veterinary use owing to its low bioavailability and poor solubility. Hence, analogs of curcuminoids with improved biological properties have been synthesized to overcome these limitations. This study aims to provide the pharmacological basis for the use of 5-(3,4-dihydroxyphenyl)-3-hydroxy-1-(2-hydroxyphenyl)penta-2,4-dien-1-one (DHHPD), a synthetic curcuminoid analog, as an anti-edematogenic and anti-granuloma agent. The carrageenan-induced paw edema and the cotton pellet-induced granuloma assays were used to assess the anti-inflammatory activity of DHHPD in mice. The effects of DHHPD on the histaminergic, serotonergic, and bradykininergic systems were determined by the histamine-, serotonin-, and bradykinin-induced paw edema tests, respectively. DHHPD (0.1, 0.3, 1, and 3 mg/kg, intraperitoneal) evoked significant reductions (p < 0.05) in carrageenan-induced paw edema at different time intervals and granuloma formation (p < 0.0001) by 22.08, 32.57, 37.20, and 49.25%, respectively. Furthermore, DHHPD significantly reduced paw edema (p < 0.05) induced by histamine, serotonin, and bradykinin. The present study suggests that DHHPD exerts anti-edematogenic activity, possibly by inhibiting the synthesis or release of autacoid mediators of inflammation through the histaminergic, serotonergic, and bradykininergic systems. The anti-granuloma effect may be attributed to the suppression of transudative, exudative, and proliferative activities associated with inflammation.
    MeSH terms: Animals; Anti-Inflammatory Agents/chemical synthesis; Anti-Inflammatory Agents/pharmacology*; Anti-Inflammatory Agents/chemistry*; Disease Models, Animal; Dose-Response Relationship, Drug; Edema/drug therapy; Edema/etiology; Granuloma/drug therapy; Granuloma/etiology; Male; Molecular Structure; Toxicity Tests, Acute; Diarylheptanoids/chemical synthesis; Diarylheptanoids/pharmacology*; Diarylheptanoids/chemistry*; Mice
  16. Sammour RMF, Taher M, Chatterjee B, Shahiwala A, Mahmood S
    Pharmaceutics, 2019 Jul 18;11(7).
    PMID: 31323799 DOI: 10.3390/pharmaceutics11070350
    In the contemporary medical model world, the proniosomal system has been serving as a new drug delivery system that is considered to significantly enhance the bioavailability of drugs with low water solubility. The application of this system can improve the bioavailability of aceclofenac that is used for the relief of pain and inflammation in osteoarthritis, rheumatoid arthritis, and ankylosing spondylitis. The present study is intended to develop an optimized proniosomal aceclofenac formula by the use of different carriers. Aceclofenac proniosomes have been prepared by slurry method, and different carriers such as maltodextrin, mannitol, and glucose were tried. Prepared proniosomes characterized by differential scanning calorimetry (DSC) analysis and Fourier transform infrared (FTIR) analysis revealed the compatibility of the drug chosen with the ingredient added, powder X-ray diffractometry (XRD) confirmed the amorphous phase of the prepared proniosomes, and finally, the surfactant layer was observed by scanning electron microscopy (SEM). Aceclofenac physical state transformations were confirmed with all formulas but maltodextrin proniosomes exhibited solubility more than other formulations. HPLC method has been used to analyze the niosomes derived from proniosomes in terms of their entrapment capability and drug content. The obtained results revealed that aceclofenac proniosomes can be successfully prepared by using different carriers.
    MeSH terms: Arthritis, Rheumatoid; Biological Availability; Calorimetry, Differential Scanning; Chromatography, High Pressure Liquid; Diclofenac; Fourier Analysis; Glucose; Inflammation; Liposomes; Mannitol; Microscopy, Electron, Scanning; Osteoarthritis; Pain; Polysaccharides; Solubility; Spondylitis, Ankylosing; Surface-Active Agents; X-Rays; Drug Delivery Systems; Spectroscopy, Fourier Transform Infrared
  17. Md Badrul Hisham NH, Ibrahim MF, Ramli N, Abd-Aziz S
    Molecules, 2019 Jul 18;24(14).
    PMID: 31323813 DOI: 10.3390/molecules24142617
    Heavy metals from industrial effluents and sewage contribute to serious water pollution in most developing countries. The constant penetration and contamination of heavy metals into natural water sources may substantially raise the chances of human exposure to these metals through ingestion, inhalation, or skin contact, which could lead to liver damage, cancer, and other severe conditions in the long term. Biosurfactant as an efficient biological surface-active agent may provide an alternative solution for the removal of heavy metals from industrial wastes. Biosurfactants exhibit the properties of reducing surface and interfacial tension, stabilizing emulsions, promoting foaming, high selectivity, and specific activity at extreme temperatures, pH, and salinity, and the ability to be synthesized from renewable resources. This study aimed to produce biosurfactant from renewable feedstock, which is used cooking oil (UCO), by a local isolate, namely Bacillus sp. HIP3 for heavy metals removal. Bacillus sp. HIP3 is a Gram-positive isolate that gave the highest oil displacement area with the lowest surface tension, of 38 mN/m, after 7 days of culturing in mineral salt medium and 2% (v/v) UCO at a temperature of 30 °C and under agitation at 200 rpm. An extraction method, using chloroform:methanol (2:1) as the solvents, gave the highest biosurfactant yield, which was 9.5 g/L. High performance liquid chromatography (HPLC) analysis confirmed that the biosurfactant produced by Bacillus sp. HIP3 consists of a lipopeptide similar to standard surfactin. The biosurfactant was capable of removing 13.57%, 12.71%, 2.91%, 1.68%, and 0.7% of copper, lead, zinc, chromium, and cadmium, respectively, from artificially contaminated water, highlighting its potential for bioremediation.
    MeSH terms: Adsorption; Bacillus/classification; Bacillus/genetics; Bacillus/metabolism*; Bacterial Proteins/genetics; Biodegradation, Environmental*; Dietary Fats, Unsaturated/metabolism*; Phylogeny; Surface-Active Agents/isolation & purification; Surface-Active Agents/metabolism*; Surface-Active Agents/chemistry*; Metals, Heavy/chemistry*
  18. Tieng FYF, Latifah SY, Md Hashim NF, Khaza'ai H, Ahmat N, Gopalsamy B, et al.
    Molecules, 2019 Jul 18;24(14).
    PMID: 31323836 DOI: 10.3390/molecules24142619
    Breast cancer is the most common and the second leading cause of cancer-related deaths in women. It has two distinctive hallmarks: rapid abnormal growth and the ability to invade and metastasize. During metastasis, cancer cells are thought to form actin-rich protrusions, called invadopodia, which degrade the extracellular matrix. Current breast cancer treatments, particularly chemotherapy, comes with adverse effects like immunosuppression, resistance development and secondary tumour formation. Hence, naturally-occurring molecules claimed to be less toxic are being studied as new drug candidates. Ampelopsin E, a natural oligostilbene extracted from Dryobalanops species, has exhibited various pharmacological properties, including anticancer and anti-inflammatory activities. However, there is yet no scientific evidence of the effects of ampelopsin E towards metastasis. Scratch assay, transwell migration and invasion assays, invadopodia and gelatin degradation assays, and ELISA were used to determine the effects of ampelopsin E towards the invasiveness of MDA-MB-231 cells. Strikingly in this study, ampelopsin E was able to halt migration, transmigration and invasion in MDA-MB-231 cells by reducing formation of invadopodia and its degradation capability through significant reduction (p < 0.05) in expression levels of PDGF, MMP2, MMP9 and MMP14. In conclusion, ampelopsin E reduced the invasiveness of MDA-MB-231 cells and was proven to be a potential alternative in treating TNBC.
    MeSH terms: Podosomes/drug effects; Antineoplastic Agents, Phytogenic/pharmacology*; Antineoplastic Agents, Phytogenic/chemistry; Cell Movement/drug effects*; Cell Survival/drug effects; Flavonoids/pharmacology*; Flavonoids/chemistry; Humans; Molecular Structure; Cell Line, Tumor; Dipterocarpaceae/chemistry; Triple Negative Breast Neoplasms
  19. Fikry M, Yusof YA, M Al-Awaadh A, Abdul Rahman R, Chin NL, Ghazali HM
    Antioxidants (Basel), 2019 Jul 18;8(7).
    PMID: 31323854 DOI: 10.3390/antiox8070226
    Full-fat roasted date seeds are considered an excellent source of antioxidants which can treat many diseases. The specific objectives were to investigate the effect of roasting temperature and time on the hardness of whole seeds, moisture content of the roasted date seeds powder, DPPH radical scavenging activity, total phenolic contents, extraction yield, pH, browning index and sensory properties of the brew prepared from the full-fat roasted date seeds and to construct descriptive models that could describe this effect. Date seeds were roasted at three temperatures (160, 180 and 200 °C) for different period of times (10, 20 and 30 min) using a natural conventional oven; then grinded and next brewed. Hardness of whole seeds, moisture content of the seeds powder, DPPH radical scavenging activity and total phenolic contents, extraction yield, pH and browning index and sensory properties of the brew were significantly affected by the roasting conditions. The statistical results indicated that the proposed model could adequately describe the measured properties. Strong correlations have been found among the properties of the brew as well. The producers of the date seeds brew can utilize these results for controlling the roasting process.
    MeSH terms: Antioxidants; Hardness; Phenols; Powders; Temperature; Gas Scavengers
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