Browse publications by year: 2019

  1. Mustafa HMJ, Ayob M, Nazri MZA, Kendall G
    PLoS One, 2019;14(5):e0216906.
    PMID: 31137034 DOI: 10.1371/journal.pone.0216906
    The performance of data clustering algorithms is mainly dependent on their ability to balance between the exploration and exploitation of the search process. Although some data clustering algorithms have achieved reasonable quality solutions for some datasets, their performance across real-life datasets could be improved. This paper proposes an adaptive memetic differential evolution optimisation algorithm (AMADE) for addressing data clustering problems. The memetic algorithm (MA) employs an adaptive differential evolution (DE) mutation strategy, which can offer superior mutation performance across many combinatorial and continuous problem domains. By hybridising an adaptive DE mutation operator with the MA, we propose that it can lead to faster convergence and better balance the exploration and exploitation of the search. We would also expect that the performance of AMADE to be better than MA and DE if executed separately. Our experimental results, based on several real-life benchmark datasets, shows that AMADE outperformed other compared clustering algorithms when compared using statistical analysis. We conclude that the hybridisation of MA and the adaptive DE is a suitable approach for addressing data clustering problems and can improve the balance between global exploration and local exploitation of the optimisation algorithm.
    MeSH terms: Machine Learning*; Computer Simulation*; Models, Theoretical*; Cluster Analysis
  2. Cheung YB, Yeo KK, Chong KJ, Khoo EYH, Wee HL
    Health Qual Life Outcomes, 2019 Apr 17;17(1):67.
    PMID: 30995918 DOI: 10.1186/s12955-019-1130-0
    BACKGROUND: The WHOQOL-BREF is a widely used questionnaire for measuring quality of life. It is important to establish the measurement equivalence of various language versions of WHOQOL-BREF so that scores from different language versions may be pooled together. The primary aim of this article was to evaluate the measurement equivalence of the English, Chinese and Malay versions of the WHOQOL-BREF.

    METHODS: We analysed data from the previously published, cross-sectional, WONDERS study and used linear regression models to adjust for potential confounding variables. Based on equivalence clinical trial methods, measurement equivalence was assessed by comparing 90% confidence interval (CI) of differences in scores across language versions with a predefined equivalence margin of 0.3 SD. Equivalence was achieved if the 90% CI fell within 0.3 SD. Data from 1203 participants, aged above 21 years, were analysed.

    RESULTS: Participants who completed the different language versions of WHOQOL-BREF expectedly differed in age, ethnicity, highest education level, marital status, smoking status and Body Mass Index (BMI). The English and Malay language versions were definitely equivalent for all domains. The English and Chinese language versions were definitely equivalent for physical and environmental domains but inconclusive for psychological and social domains. Likewise, for Chinese and Malay versions.

    CONCLUSION: The English, Chinese and Malay language versions of the WHOQOL-BREF questionnaire may be considered equivalent, with evidence being more robust for some domains than the others. Given the large number of people who speak/ read Chinese and Malay, this study has widespread relevance.

    MeSH terms: Adult; Aged; Cross-Sectional Studies; Female; Humans; Malaysia; Male; Middle Aged; Psychometrics/methods; Quality of Life*; Surveys and Questionnaires/standards*; Translations*; World Health Organization; Linear Models; Young Adult
  3. Chin YX, Mi Y, Cao WX, Lim PE, Xue CH, Tang QJ
    Nutrients, 2019 May 21;11(5).
    PMID: 31117266 DOI: 10.3390/nu11051133
    Kappaphycus is a commercially important edible red alga widely cultivated for carrageenan production. Here, we aimed to investigate the anti-obesity mechanism of Kappaphycusalvarezii by comparing the effects of whole seaweed (T), extracted native κ-carrageenan (CGN), and the leftover fraction sans-carrageenan (SCGN) supplementations (5%, w/w) on diet-induced obese C57BL/6J mice. A high-fat diet induced both a raised body fat percentage and serum cholesterol level, increased adipocytes size, abnormal levels of adipocytokines, and promoted gut dysbiosis. Our results showed that, overall, both CGN and SCGN were more effective in reversing obesity and related metabolic syndromes to normal levels than T. Furthermore, these findings suggested that CGN- and SCGN-modulated gut dysbiosis induced by a high-fat diet, which may play an influencing role in adiponectin dysregulation. Our data also showed some evidence that CGN and SCGN have distinct effects on selected genes involved in lipid metabolism. In conclusion, both κ-carrageenan and SCGN have novel anti-obesity potential with possible different mechanisms of action.
    MeSH terms: Gastrointestinal Microbiome; Rhodophyta/chemistry*; Animals; Carrageenan/pharmacology*; Carrageenan/chemistry; Gene Expression Regulation/drug effects; Male; Mice, Inbred C57BL; Obesity/chemically induced*; Obesity/drug therapy*; Pilot Projects; Plant Extracts/pharmacology*; Plant Extracts/chemistry; Specific Pathogen-Free Organisms; Anti-Obesity Agents/pharmacology*; Anti-Obesity Agents/chemistry; Lipid Metabolism; Mice; Adipokines/metabolism; Diet, High-Fat/adverse effects
  4. Al-Madhagi WM, Hashim NM, Awadh Ali NA, Taha H, Alhadi AA, Abdullah AA, et al.
    J Chem Inf Model, 2019 05 28;59(5):1858-1872.
    PMID: 31117526 DOI: 10.1021/acs.jcim.8b00969
    Bioassay-guided isolation protocol was performed on petroleum ether extract of Peperomia blanda (Jacq.) Kunth using column chromatographic techniques. Five compounds were isolated and their structures were elucidated via one-dimensional (1D) and two-dimensional (2D) NMR, gas chromatography mass sectroscopy (GCMS), liquid chromatography mass spectroscopy (LCMS), and ultraviolet (UV) and infrared (IR) analyses. Dindygulerione E (a new compound), and two compounds isolated from P. blanda for the first time-namely, dindygulerione A and flavokawain A-are reported herein. Antimicrobial activity was screened against selected pathogenic microbes, and minimum inhibitory concentrations (MIC) were recorded within the range of 62-250 μg/mL. Assessment of the pharmacotherapeutic potential has also been done for the isolated compounds, using the Prediction of Activity spectra for Substances (PASS) software, and different activities of compounds were predicted. Molecular docking, molecular dynamics simulation and molecular mechanics/Poisson-Boltzmann Surface Area (MM-PBSA) calculations have proposed the binding affinity of these compounds toward methylthioadenosine phosphorylase enzyme, which may explain their inhibitory actions.
    MeSH terms: Anti-Bacterial Agents/isolation & purification; Anti-Bacterial Agents/pharmacology*; Anti-Bacterial Agents/chemistry*; Bacteria/drug effects; Bacterial Infections/drug therapy; Humans; Microbial Sensitivity Tests; Models, Molecular; Petroleum/analysis; Plant Extracts/isolation & purification; Plant Extracts/pharmacology*; Plant Extracts/chemistry*; Peperomia/chemistry*
  5. Abdullah NM, Mohammad WMZW, Shafei MN, Sukeri S, Idris Z, Arifin WN, et al.
    BMC Public Health, 2019 May 22;19(1):628.
    PMID: 31117995 DOI: 10.1186/s12889-019-6981-0
    BACKGROUND: Leptospirosis is still endemic in Malaysia and has been prevalent in Selangor where cases have been underreported. Primarily, this was due to lack of awareness in the urban community in this region. This study determined the knowledge, attitude and preventive practice (KAP) of leptospirosis, and identified the significant predictors influencing KAP among urban community in Hulu Langat, Selangor.

    METHODS: This cross-sectional study was conducted from 2015 to 2017 using validated questionnaire. Sampling methods included multistage cluster sampling, followed by simple random sampling to obtain 315 respondents. Descriptive analysis was performed to determine the KAP while χ2 and the subsequent logistic regression analysis were carried out to identify associations and predictors between variables.

    RESULTS: Respondents were mainly Malaysian Bumiputra with a mean (Standard Deviation (SD)) age of 32.5 (13.0) years. Of 315 respondents, 80.3% (n = 253) had poor knowledge, 87.0% (n = 274) had good attitude, and 81.3% (n = 256) showed unacceptable practice towards leptospirosis and its prevention. Regression analysis identified age as the sole predictor influencing good knowledge (AOR 2.388; 95% CI = 1.298, 4.396; p = 0.005). Education level (AOR 2.197; 95% CI = 1.109, 4.352; p = 0.024) was also noted as the significant predictor influencing the overall practice.

    CONCLUSIONS: The urban community in Selangor showed a positive attitude in waste management despite having little knowledge regarding the disease itself. The study also discovered inadequacy in preventive practice, hence marking the importance of the proper integration of knowledge and attitude into forming an acceptable practice to reduce transmission of Leptospira among urban population in Malaysia.

    MeSH terms: Adult; Cross-Sectional Studies; Female; Humans; Health Knowledge, Attitudes, Practice*; Leptospirosis/prevention & control; Leptospirosis/psychology*; Malaysia; Male; Middle Aged; Surveys and Questionnaires; Urban Population*; Health Behavior; Logistic Models
  6. Osman J, Tan SC, Lee PY, Low TY, Jamal R
    J Biomed Sci, 2019 May 22;26(1):39.
    PMID: 31118017 DOI: 10.1186/s12929-019-0535-8
    Sudden cardiac death (SCD) is a sudden, unexpected death that is caused by the loss of heart function. While SCD affects many patients suffering from coronary artery diseases (CAD) and heart failure (HF), a considerable number of SCD events occur in asymptomatic individuals. Certain risk factors for SCD have been identified and incorporated in different clinical scores, however, risk stratification using such algorithms is only useful for health management rather than for early detection and prediction of future SCD events in high-risk individuals. In this review, we discuss different molecular biomarkers that are used for early detection of SCD. This includes genetic biomarkers, where the majority of them are genomic variants for genes that encode for ion channels. Meanwhile, protein biomarkers often denote proteins that play roles in pathophysiological processes that lead to CAD and HF, notably (i) atherosclerosis that involves oxidative stress and inflammation, as well as (ii) cardiac tissue damage that involves neurohormonal and hemodynamic regulation and myocardial stress. Finally, we outline existing challenges and future directions including the use of OMICS strategy for biomarker discovery and the multimarker panels.
    MeSH terms: Algorithms; Coronary Artery Disease; Heart Failure; Hemodynamics; Humans; Inflammation; Ion Channels; Myocardium; Risk Factors; Biomarkers; Death, Sudden, Cardiac; Oxidative Stress; Genomics; Atherosclerosis
  7. Lee YY, Kamarudin KS, Wan Muda WAM
    BMC Public Health, 2019 May 22;19(1):621.
    PMID: 31118019 DOI: 10.1186/s12889-019-6971-2
    BACKGROUND: For the past decades, Malaysia has seen an increased prevalence of overweight and obesity which leads to significant health threats. Physical activity is beneficial in maintaining healthy body weight. The objective of this study was to measure physical activity of adults in Malaysia using objective measurement (accelerometer) and self-reported methods, as well as to determine their associations with (body mass index (BMI) and waist circumference (WC) measurements.

    METHODS: Four-hundred and ninety Malaysian adults (n = 490) aged 20 to 65 years old participated in this cross-sectional study. Their body weight, height, and WC measurements were measured according to standard procedures. Physical activity was assessed objectively with accelerometers for five to seven consecutive days. The International Physical Activity Questionnaire (IPAQ) was used to estimate the amount of time spent on various domains of physical activity. Mixed models were used to determine the associations between physical activity variables and both BMI and WC.

    RESULTS: The mean value of objectively measured moderate-to-vigorous physical activity (MVPA) was 13.5 min per day, in which male participants recorded a significantly higher amount of time compared to females. On the other hand, the mean self-reported total physical activity was 380 min per week; male participants reported a significantly higher amount of time on physical activity in the occupation/work and leisure/recreation domains while female participants spent significantly more time in the domestic/household chores domain. We also observed that the mean values of objectively measured total MVPA, self-reported time spent on walking for leisure/recreation, and total time amount of time spent on MVPA for leisure/recreation were significantly higher among participants with BMI of less than 25 kg/m2. The final statistical model yielded a significant negative association between objectively measured total MVPA and BMI, but not with WC measurement. No significant association was reported between self-reported total physical activity with BMI and WC measurement.

    CONCLUSIONS: Objectively measured MVPA was inversely associated with BMI, but not WC measurement. No significant association was observed between self-reported total physical activity and physical activity time measures across domains with both BMI and WC measurement.

    MeSH terms: Adult; Aged; Body Weight; Cross-Sectional Studies; Female; Humans; Leisure Activities; Malaysia/epidemiology; Male; Middle Aged; Obesity/epidemiology*; Obesity/psychology; Exercise/psychology*; Body Mass Index; Prevalence; Walking; Overweight/epidemiology*; Overweight/psychology; Waist Circumference; Young Adult; Self Report; Accelerometry/statistics & numerical data*
  8. Mustaffa-Kamal F, Liu H, Pedersen NC, Sparger EE
    BMC Vet Res, 2019 May 22;15(1):165.
    PMID: 31118053 DOI: 10.1186/s12917-019-1909-6
    BACKGROUND: Feline infectious peritonitis (FIP) is considered highly fatal in its naturally occurring form, although up to 36% of cats resist disease after experimental infection, suggesting that cats in nature may also resist development of FIP in the face of infection with FIP virus (FIPV). Previous experimental FIPV infection studies suggested a role for cell-mediated immunity in resistance to development of FIP. This experimental FIPV infection study in specific pathogen free (SPF) kittens describes longitudinal antiviral T cell responses and clinical outcomes ranging from rapid progression, slow progression, and resistance to disease.

    RESULTS: Differences in disease outcome provided an opportunity to investigate the role of T cell immunity to FIP determined by T cell subset proliferation after stimulation with different viral antigens. Reduced total white blood cell (WBC), lymphocyte and T cell counts in blood were observed during primary acute infection for all experimental groups including cats that survived without clinical FIP. Antiviral T cell responses during early primary infection were also similar between cats that developed FIP and cats remaining healthy. Recovery of antiviral T cell responses during the later phase of acute infection was observed in a subset of cats that survived longer or resisted disease compared to cats showing rapid disease progression. More robust T cell responses at terminal time points were observed in lymph nodes compared to blood in cats that developed FIP. Cats that survived primary infection were challenged a second time to pathogenic FIPV and tested for antiviral T cell responses over a four week period. Nine of ten rechallenged cats did not develop FIP or T cell depletion and all cats demonstrated antiviral T cell responses at multiple time points after rechallenge.

    CONCLUSIONS: In summary, definitive adaptive T cell responses predictive of disease outcome were not detected during the early phase of primary FIPV infection. However emergence of antiviral T cell responses after a second exposure to FIPV, implicated cellular immunity in the control of FIPV infection and disease progression. Virus host interactions during very early stages of FIPV infection warrant further investigation to elucidate host resistance to FIP.

    MeSH terms: Animals; Antigens, Viral/immunology; Cats; Immunity, Cellular*; Specific Pathogen-Free Organisms; T-Lymphocytes/immunology*; Coronavirus, Feline/immunology*; Feline Infectious Peritonitis/immunology*; Disease Resistance/immunology
  9. Benchoula K, Khatib A, Jaffar A, Ahmed QU, Sulaiman WMAW, Wahab RA, et al.
    Exp Anim, 2019 Nov 06;68(4):407-416.
    PMID: 31118344 DOI: 10.1538/expanim.18-0168
    Metabolic syndrome is a cluster including hyperglycaemia, obesity, hypertension, and hypertriglyceridaemia as a result of biochemical and physiological alterations and can increase the risk of cardiovascular disease and diabetes. Fundamental research on this disease requires validated animal models. One potential animal model that is rapidly gaining in popularity is zebrafish (Danio rerio). The use of zebrafish as an animal model conveys several advantages, including high human genetic homology, transparent embryos and larvae that allow easier visualization. This review discusses how zebrafish models contribute to the development of metabolic syndrome studies. Different diseases in the cluster of metabolic syndrome, such as hyperglycaemia, obesity, diabetes, and hypertriglyceridaemia, have been successfully studied using zebrafish; and the model is promising for hypertension and cardiovascular metabolic-related diseases due to its genetic similarity to mammals. Genetic mutation, chemical induction, and dietary alteration are among the tools used to improve zebrafish models. This field is expanding, and thus, more effective and efficient techniques are currently developed to fulfil the increasing demand for thorough investigations.
    MeSH terms: Animals; Diabetes Mellitus/etiology*; Disease Models, Animal*; Hyperglycemia/etiology*; Obesity/etiology*; Zebrafish*; Hypertriglyceridemia/etiology*; Metabolic Syndrome X/etiology*
  10. Yusoff FM, Kajikawa M, Matsui S, Hashimoto H, Kishimoto S, Maruhashi T, et al.
    Sci Rep, 2019 05 22;9(1):7711.
    PMID: 31118440 DOI: 10.1038/s41598-019-44176-5
    Critical limb ischemia (CLI) is associated with a high risk of limb amputation. It has been shown that cell therapy is safe and has beneficial effects on ischemic clinical symptoms in patients with CLI. The aim of this study was to further investigate the outcomes of intramuscular injection of autologous bone-marrow mononuclear cells (BM-MNCs) in a long-term follow-up period in atherosclerotic peripheral arterial disease (PAD) patients who have no optional therapy. This study was a retrospective and observational study that was carried out to evaluate long-term clinical outcomes in 42 lower limbs of 30 patients with atherosclerotic PAD who underwent BM-MNC implantation. The median follow-up period was 9.25 (range, 6-16) years. The overall amputation-free rates were 73.0% at 5 years after BM-MNC implantation and 70.4% at 10 years in patients with atherosclerotic PAD. The overall amputation-free rates at 5 years and at 10 years after implantation of BM-MNCs were significantly higher in atherosclerotic PAD patients than in internal controls and historical controls. There were no significant differences in amputation rates between the internal control group and historical control group. The rate of overall survival was not significantly different between the BM-MNC implantation group and the historical control group. Implantation of autologous BM-MNCs is feasible for a long-term follow-up period in patients with CLI who have no optional therapy.
    MeSH terms: Aged; Aged, 80 and over; Amputation; Female; Follow-Up Studies; Humans; Injections, Intramuscular; Ischemia/etiology; Ischemia/surgery; Ischemia/therapy*; Leg/blood supply*; Male; Middle Aged; Monocytes/transplantation*; Retrospective Studies; Transplantation, Autologous; Comorbidity; Bone Marrow Transplantation*; Treatment Outcome; Kaplan-Meier Estimate; Peripheral Arterial Disease/complications
  11. Arshad L, Jantan I, Bukhari SNA
    Drug Des Devel Ther, 2019;13:1421-1436.
    PMID: 31118577 DOI: 10.2147/DDDT.S185191
    Background: 3,5-Bis[4-(diethoxymethyl)benzylidene]-1-methyl-piperidin-4-one (BBP), a novel synthetic curcumin analogue has been revealed to possess strong in vitro and in vivo immunosuppressive effects. Purpose: The aim of present study was to prepare and characterize BBP-encapsulated polylactic-co-glycolic acid-block-polyethylene glycol (PLGA-b-PEG) nanoparticles and to evaluate its in vivo efficacy against innate and adaptive immune responses. Methods: Male BALB/c mice were orally administered with BBP alone and BBP- encapsulated nanoparticles equivalent to 5, 10 and 20 mg/kg of BBP in distilled water for a period of 14 days. The immunomodulatory potential was appraised by determining its effects on non-specific and specific immune parameters. Results: The results showed that BBP was successfully encapsulated in PLGA-b-PEG polymer with 154.3 nm size and high encapsulation efficiency (79%) while providing a sustained release for 48 hours. BBP nanoparticles showed significant enhanced dose-dependent reduction on the migration of neutrophils, Mac-1 expression, phagocytic activity, reactive oxygen species (ROS) production, serum levels of ceruloplasmin and lysozyme, immunoglobulins and myloperoxidase (MPO) plasma levels when compared to unencapsulated BBP. Enhanced dose-dependent inhibition was also observed on lymphocyte proliferation along with the downregulation of effector cells expression and release of cytokines, and reduction in rat paw oedema in BBP nanoparticles treated mice. At higher doses the suppressive effects of the BBP nanoparticles on various cellular and humoral parameters of immune responses were comparable to that of cyclosporine-A at 20 mg/kg. Conclusion: These findings suggest that the immunosuppressive effects of BBP were enhanced as PLGA-b-PEG nanoparticles.
    MeSH terms: Animals; Curcumin/analogs & derivatives*; Curcumin/chemical synthesis; Curcumin/pharmacology; Curcumin/chemistry; Dose-Response Relationship, Drug; Immunity, Innate/drug effects*; Immunity, Innate/immunology; Immunosuppressive Agents/chemical synthesis; Immunosuppressive Agents/pharmacology*; Immunosuppressive Agents/chemistry; Male; Mice, Inbred BALB C; Piperidines/chemical synthesis; Piperidines/pharmacology*; Piperidines/chemistry; Polyethylene Glycols/chemistry*; Polyglactin 910/chemistry*; Sheep; Structure-Activity Relationship; Molecular Structure; Mice; Nanoparticles/chemistry*; Adaptive Immunity/drug effects*; Adaptive Immunity/immunology
  12. Zin CS, Alias NE, Taufek NH, Ahmad MM
    J Pain Res, 2019;12:1251-1257.
    PMID: 31118748 DOI: 10.2147/JPR.S199243
    Purpose: This study evaluated the risk of opioid dose escalation as it relates to sex differences among patients receiving opioids for long-term therapy. Patients and methods: This retrospective cohort study was conducted in tertiary hospital settings in Malaysia using electronic prescription records. Opioid naïve patients, aged ≥18 years, who were undergoing long-term opioid therapy of ≥90 days, with at least one opioid prescription (buprenorphine, morphine, oxycodone, fentanyl, dihydrocodeine or tramadol) between 1st January 2011 and 31st December 2016, were included in the study. They were followed until (i) the end of the study period, (ii) death from any cause or (iii) discontinuation of therapy from their first opioid prescription without any intervals of ≥120 days between successive prescriptions. The risk of high opioid dose escalation to ≥100 mg/day and ≥200 mg/day relative to men and women was measured. Results: A total of 4688 patients (58.8% women, 41.3% men) on long-term opioid therapy were identified. Among these patients, 248 (5.29%) were escalated to high opioid doses of ≥100 mg/day and 69 (1.47%) were escalated to ≥200 mg/day. The escalation to high-dose opioid therapy was more likely to occur in men than in women, even after adjustment for age (dose ≥100 mg/day [adjusted hazard ratio 2.32; 95% confidence interval (CI), 1.79 to 3.00; p<0.0001] and ≥200 mg/day [adjusted hazard ratio 6.10; 95% CI, 3.39 to 10.98; p<0.0001]). Conclusion: The risk of opioid dose escalation differed between men and women, as men were at higher risk than women for high opioid dose escalation.
  13. Ceesay A, Nor Shamsudin M, Aliyu-Paiko M, Ismail IS, Nazarudin MF, Mohamed Alipiah N
    Biomed Res Int, 2019;2019:2640684.
    PMID: 31119160 DOI: 10.1155/2019/2640684
    The aim of the present study was to extract and characterize bioactive components from separate body organs of Holothuria leucospilota. Preliminary qualitative assessment of the crude extracts was positive for phenols, terpenoids, carbohydrates, flavonoids, saponins, glycosides, cardiac glycosides, steroids, phlobatannins, and tannins in all body organs evaluated. Phenolics were the most abundant group of bioactives accounting for approximately 80%. The extraction solvent mixtures that yielded most compounds evaluated were methanol/acetone (3:1, v:v) and methanol/distilled water (3:1, v:v). In other analyses, GC-MS data revealed diverse metabolic and biologically active compounds, where those in high concentrations included 2-Pentanone, 4-hydroxy-4-methyl- among the ketones; phenol- 2,4-bis(1,1-dimethylethyl)-, a phenol group; and 2-Chlorooctane, a hydrocarbon. Among FA and their methyl/ethyl esters, n-hexadecanoic acid, 5,8,11,14-eicosatetraenoic acid ethyl ester (arachidonic acid), and 5,8,11,14,17-eicosapentaenoic acid methyl ester (EPA) were among the most abundant FAMEs accounting for approximately 50% of the subgroups measured. Data from GC-FID analysis revealed methyl laurate (C12:0), methyl myristate (C14:0), methyl palmitate (C16:0), and methyl stearate (18:0) methyl esters as the most abundant saturated FA, whereas cis-9-oleic methyl ester (C18:1) and methyl linoleate (C18:2) were found as the major monounsaturated FA and PUFA FAMEs, respectively, in the body wall of the species. Taken together, the extraction and characterization of different categories of metabolically and biologically active compounds in various organ extracts of H. leucospilota suggest that the species is potentially a rich source of cholesterol-lowering, antioxidant, antimicrobial, and anticancer agents. These substances are known to benefit human health and assist in disease prevention. These findings justify the use of sea cucumbers in traditional folklore medication and the current interest and attention focused on the species to mine for bioactives in new drugs research.
    MeSH terms: Animals; Anti-Infective Agents/therapeutic use; Anti-Infective Agents/chemistry*; Antioxidants/therapeutic use; Antioxidants/chemistry*; Fatty Acids, Monounsaturated/therapeutic use; Fatty Acids, Monounsaturated/chemistry*; Flavonoids/chemistry; Glycosides/chemistry; Humans; Gas Chromatography-Mass Spectrometry; Saponins/chemistry; Sea Cucumbers/chemistry*; Terpenes/chemistry
  14. Bilal M, Anis H, Khan N, Qureshi I, Shah J, Kadir KA
    Biomed Res Int, 2019;2019:6139785.
    PMID: 31119178 DOI: 10.1155/2019/6139785
    Background: Motion is a major source of blurring and ghosting in recovered MR images. It is more challenging in Dynamic Contrast Enhancement (DCE) MRI because motion effects and rapid intensity changes in contrast agent are difficult to distinguish from each other.

    Material and Methods: In this study, we have introduced a new technique to reduce the motion artifacts, based on data binning and low rank plus sparse (L+S) reconstruction method for DCE MRI. For Data binning, radial k-space data is acquired continuously using the golden-angle radial sampling pattern and grouped into various motion states or bins. The respiratory signal for binning is extracted directly from radially acquired k-space data. A compressed sensing- (CS-) based L+S matrix decomposition model is then used to reconstruct motion sorted DCE MR images. Undersampled free breathing 3D liver and abdominal DCE MR data sets are used to validate the proposed technique.

    Results: The performance of the technique is compared with conventional L+S decomposition qualitatively along with the image sharpness and structural similarity index. Recovered images are visually sharper and have better similarity with reference images.

    Conclusion: L+S decomposition provides improved MR images with data binning as preprocessing step in free breathing scenario. Data binning resolves the respiratory motion by dividing different respiratory positions in multiple bins. It also differentiates the respiratory motion and contrast agent (CA) variations. MR images recovered for each bin are better as compared to the method without data binning.

    MeSH terms: Algorithms; Contrast Media/therapeutic use; Humans; Image Enhancement/methods*; Image Interpretation, Computer-Assisted/methods*; Image Processing, Computer-Assisted/methods*; Magnetic Resonance Imaging/methods*; Motion; Respiration; Artifacts
  15. Mahmoudian MR, Basirun WJ, Woi PM, Hazarkhani H, Alias YB
    Mikrochim Acta, 2019 05 22;186(6):369.
    PMID: 31119482 DOI: 10.1007/s00604-019-3481-y
    The study presents the synthesis of polypyrrole-coated palladium platinum/nitrogen-doped reduced graphene oxide nanocomposites (PdPt-PPy/N-rGO NC) via direct the reduction of Pd(II) and Pt(II) in the presence of pyrrole monomer, N-rGO and L-cysteine as the reducing agent. X-ray diffraction confirmed the presence of metallic Pd and Pt from the reduction of Pd and Pt cations. Transmission electron microscopy images revealed the presence of Pd, Pt and PPy deposition on N-rGO. Impedance spectroscopy results gave a decreased charge transfer resistance due to the presence of N-rGO. The nanocomposites were synthesized with different Pd/Pt ratios (2:1, 1:1 and 1:2). A glassy carbon electrode (GCE) modified with the nanocomposite showed enhanced electrochemical sensing capability for formaldehyde in 0.1 M sulfuric acid solution. Cyclic voltammetry showed an increase in the formaldehyde oxidation peak current at the GCE modified with Pd2Pt1 PPy N-rGO. At a typical potential of 0.45 V (vs. SCE), the sensitivity in the linear segment was 345.8 μA.mM -1. cm-2. The voltammetric response was linear between 0.01 and 0.9 mM formaldehyde concentration range, with a 27 µM detection limit (at S/N = 3). Graphical abstract Schematic presentation of formaldehyde detection by Pd2Pt1-PPy/nitrogen-doped reduced Graphene Oxide Nanocomposite (Pd2Pt1-PPy /N-Gr NC). The decrease of charge transfer resistance and the agglomeration of deposited metals in the presence of N-rGO enhance the current response of the electrochemical sensor.
    MeSH terms: Carbon; Cations; Cysteine; Electrodes; Formaldehyde; Graphite; Nitrogen; Oxides; Palladium; Platinum; Polymers; Pyrroles; Sulfuric Acids; X-Ray Diffraction; Reducing Agents; Microscopy, Electron, Transmission; Nanocomposites; Limit of Detection; Dielectric Spectroscopy
  16. Arora S, Kanneppady SK, Banavar SR, Jnanendrappa N
    QJM, 2019 Aug 01;112(8):615-616.
    PMID: 31120127 DOI: 10.1093/qjmed/hcz117
    MeSH terms: Adult; Ameloblastoma/diagnosis*; Ameloblastoma/pathology; Ameloblastoma/surgery; Hemangioma/diagnosis*; Hemangioma/pathology; Hemangioma/surgery; Humans; Male; Mandibular Neoplasms/diagnosis*; Mandibular Neoplasms/pathology; Mandibular Neoplasms/surgery; Cone-Beam Computed Tomography
  17. Yap KH, Warren N, Reidpath DD, Allotey P
    Int J Qual Stud Health Well-being, 2019 Dec;14(1):1613875.
    PMID: 31120385 DOI: 10.1080/17482631.2019.1613875
    Purpose: Stroke survivors report poorer self-rated health (SRH) compared to the general population but there is limited understanding on what contributes to SRH. This ethnographic study examined the individual and contextual factors that shape stroke survivors' SRH in a rural middle income country situated in South East Asia. Methods: Ethnographic methods which encompasses various data collection methods from different data sources were used in this study to describe the socio-cultural context of 16 stroke survivors living in a rural village. Within this context, the experiences of these participants were then interpreted in terms of what contributed to their perception of health and recovery, juxtaposed with objectively measure physical and cognitive states. Results: SRH reflected the post stroke adjustment of stroke survivors. Better SRH was influenced by good post-stroke adjustment that was achieved by a combination of physical functioning, cognitive functioning, emotional well-being and family support. Poorer SRH appear to reflect poor post-stroke adjustment regardless of the objective physical and cognitive states of the stroke survivors. It was also observed that cognitive deficits, though its presence was acknowledged by participants, were usually not taken into account when rating SRH. However, while physical functioning was perceived by participants to directly impact SRH, the presence of cognitive deficits (often in tandem with depressive symptoms) indirectly complicated the recovery of physical functions treasured by participants. Conclusion: Stroke survivors reporting poorer SRH warrant further attention and intervention from health practitioners supporting the longer-term needs of stroke survivors in similar settings.
    MeSH terms: Aged; Aged, 80 and over; Female; Health Status*; Humans; Male; Middle Aged; Rural Population*; Survivors/psychology*; Stroke*; Self Report
  18. Loganathan K, Moriya S, Parhar IS
    Zoolog Sci, 2019 04 01;36(2):167-171.
    PMID: 31120653 DOI: 10.2108/zs180111
    The two-pore domain potassium ion (K + ) channel-related K + (TREK) channel and melatonin receptors play roles in the regulation of reproduction in zebrafish. Since reproduction is regulated by diurnal rhythms, the TREK family and melatonin receptors may exhibit diurnal rhythms in expression. In this study, we aimed to investigate diurnal variations of the gene expressions of TREK family and melatonin receptors and their associations with kisspeptin and gonadotrophin-releasing hormone (GnRH). Diurnal variations of trek1b, trek2a, trek2b, mt1, mt2, mel1a, kiss2 and gnrh3 expressions were examined by real-time PCR. For reproduction-related genes, kiss2 and gnrh3 exhibited diurnal rhythms. trek2a revealed a diurnal rhythm in the TREK family. mt2 and mel1c exhibited diurnal rhythms in the melatonin receptors. Since Trek2a regulates gnrh3 expression, the diurnal rhythm of gnrh3 expression suggests to be regulated by the diurnal rhythm of trek2a expression.
    MeSH terms: Animals; Circadian Rhythm/genetics*; Circadian Rhythm/physiology; Gene Expression Regulation/physiology; Gonadotropin-Releasing Hormone/genetics; Gonadotropin-Releasing Hormone/metabolism*; Male; Pyrrolidonecarboxylic Acid/analogs & derivatives*; Pyrrolidonecarboxylic Acid/metabolism; Zebrafish/genetics; Zebrafish/metabolism*; Potassium Channels, Tandem Pore Domain/genetics; Potassium Channels, Tandem Pore Domain/metabolism*; Receptors, Melatonin/genetics; Receptors, Melatonin/metabolism; Kisspeptins/metabolism; Real-Time Polymerase Chain Reaction
  19. Rahim LA, Kudiri KM, Bahattacharjee S
    PLoS One, 2019;14(5):e0214044.
    PMID: 31120878 DOI: 10.1371/journal.pone.0214044
    The parallelisation of big data is emerging as an important framework for large-scale parallel data applications such as seismic data processing. The field of seismic data is so large or complex that traditional data processing software is incapable of dealing with it. For example, the implementation of parallel processing in seismic applications to improve the processing speed is complex in nature. To overcome this issue, a simple technique which that helps provide parallel processing for big data applications such as seismic algorithms is needed. In our framework, we used the Apache Hadoop with its MapReduce function. All experiments were conducted on the RedHat CentOS platform. Finally, we studied the bottlenecks and improved the overall performance of the system for seismic algorithms (stochastic inversion).
    MeSH terms: Algorithms; Software
  20. Vincent AT, Schiettekatte O, Goarant C, Neela VK, Bernet E, Thibeaux R, et al.
    PLoS Negl Trop Dis, 2019 May;13(5):e0007270.
    PMID: 31120895 DOI: 10.1371/journal.pntd.0007270
    The causative agents of leptospirosis are responsible for an emerging zoonotic disease worldwide. One of the major routes of transmission for leptospirosis is the natural environment contaminated with the urine of a wide range of reservoir animals. Soils and surface waters also host a high diversity of non-pathogenic Leptospira and species for which the virulence status is not clearly established. The genus Leptospira is currently divided into 35 species classified into three phylogenetic clusters, which supposedly correlate with the virulence of the bacteria. In this study, a total of 90 Leptospira strains isolated from different environments worldwide including Japan, Malaysia, New Caledonia, Algeria, mainland France, and the island of Mayotte in the Indian Ocean were sequenced. A comparison of average nucleotide identity (ANI) values of genomes of the 90 isolates and representative genomes of known species revealed 30 new Leptospira species. These data also supported the existence of two clades and 4 subclades. To avoid classification that strongly implies assumption on the virulence status of the lineages, we called them P1, P2, S1, S2. One of these subclades has not yet been described and is composed of Leptospira idonii and 4 novel species that are phylogenetically related to the saprophytes. We then investigated genome diversity and evolutionary relationships among members of the genus Leptospira by studying the pangenome and core gene sets. Our data enable the identification of genome features, genes and domains that are important for each subclade, thereby laying the foundation for refining the classification of this complex bacterial genus. We also shed light on atypical genomic features of a group of species that includes the species often associated with human infection, suggesting a specific and ongoing evolution of this group of species that will require more attention. In conclusion, we have uncovered a massive species diversity and revealed a novel subclade in environmental samples collected worldwide and we have redefined the classification of species in the genus. The implication of several new potentially infectious Leptospira species for human and animal health remains to be determined but our data also provide new insights into the emergence of virulence in the pathogenic species.
    MeSH terms: Animals; Asia; Humans; Leptospira/classification*; Leptospira/genetics; Leptospira/isolation & purification; Leptospira/pathogenicity*; Leptospirosis/microbiology*; Phylogeny; Virulence; Zoonoses/microbiology; Genome, Bacterial*; Evolution, Molecular*; Genomics
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