Browse publications by year: 2024

  1. Chaudhry G, Zeenia, Safdar N, Begum S, Akim AM, Sung YY, et al.
    Braz J Biol, 2024;84:e284409.
    PMID: 39699393 DOI: 10.1590/1519-6984.284409
    The importance of cytotoxicity assays in in vitro drug discovery investigations has led to their rising profile. Drugs and other substances can disrupt cell membranes, limit protein synthesis, and bind irreversibly to receptors, all of which lead to cell death in cancer cells. To precisely measure the cell death resulting from these damages, one must choose a cytotoxicity test that meets specific criteria. A systematic search strategy was used to gather grey literature from 2001 to 2024, utilizing databases such as PubMed and Google Scholar. Specific keywords related to colorimetric, fluorometric, and dye exclusion assays, as well as "cytotoxicity," were employed. Here, we only focus on screening drug cytotoxicity for cancer cells. This review discusses various cytotoxicity assays, such as "dye exclusion assays," "colorimetric assays," and "fluorometric assays." It is crucial to prioritize safety, speed, reliability, efficiency, and cost-effectiveness, while also ensuring minimal interference with the test compound. Commonly used in toxicology and pharmacology, cytotoxicity assays are based on several biological processes. Selecting the correct assay method requires considerations such as assay specificity and sensitivity, detection mechanism, test drug properties, and laboratory availability. This review aims to assist researchers in performing reliable cytotoxicity assessments by providing insights into assay choices.
    MeSH terms: Animals; Colorimetry/methods; Drug Screening Assays, Antitumor*; Fluorometry/methods; Humans; Neoplasms/drug therapy; Reproducibility of Results; Drug Discovery*
  2. Pitafi S, Anwar T, Widia IDM, Sharif Z, Yimwadsana B
    PLoS One, 2024;19(12):e0313890.
    PMID: 39700114 DOI: 10.1371/journal.pone.0313890
    Perimeter Intrusion Detection Systems (PIDS) are crucial for protecting any physical locations by detecting and responding to intrusions around its perimeter. Despite the availability of several PIDS, challenges remain in detection accuracy and precise activity classification. To address these challenges, a new machine learning model is developed. This model utilizes the pre-trained InceptionV3 for feature extraction on PID intrusion image dataset, followed by t-SNE for dimensionality reduction and subsequent clustering. When handling high-dimensional data, the existing Density-Based Spatial Clustering of Applications with Noise (DBSCAN) algorithm faces efficiency issues due to its complexity and varying densities. To overcome these limitations, this research enhances the traditional DBSCAN algorithm. In the enhanced DBSCAN, distances between minimal points are determined using an estimation for the epsilon values with the Manhattan distance formula. The effectiveness of the proposed model is evaluated by comparing it to state-of-the-art techniques found in the literature. The analysis reveals that the proposed model achieved a silhouette score of 0.86, while comparative techniques failed to produce similar results. This research contributes to societal security by improving location perimeter protection, and future researchers can utilize the developed model for human activity recognition from image datasets.
    MeSH terms: Machine Learning*; Algorithms*; Humans; Image Processing, Computer-Assisted/methods; Cluster Analysis
  3. Alshammari H, Al-Saeed E, Ahmed Z, Aslanpour Z
    PLoS One, 2024;19(12):e0311853.
    PMID: 39700210 DOI: 10.1371/journal.pone.0311853
    Deprescribing is defined as the reduction of medications to improve patient care. For effective deprescribing regular evaluation of medication adjustment regimens is required as it is documented to be an effective method to reduce polypharmacy and potentially inappropriate medications while improving patient well-being. Several factors, including patient-related aspects, influence the deprescribing process. Among these factors, patient willingness plays a pivotal role, making it essential to better understand their perspectives and attitudes towards medication use and deprescribing to successfully implement and maintain a deprescribing approach. We investigated the attitudes of older patients attending geriatric clinics in Kuwait toward deprescribing and identified predictors that influence their willingness to undergo this process. We enrolled patients aged ≥65 years who were attending geriatric clinics in primary care settings in Kuwait. These participants completed the revised Arabic version of the Patients' Attitudes Towards Deprescribing (rPATD) questionnaire. The questionnaire was designed to assess the participants' willingness to participate actively in medication decision-making and their inclination toward discontinuing certain medicines. Descriptive statistics was applied to gain insight into the characteristics of the participants and their responses to the rPATD questionnaire. Binary logistic regression identified predictors influencing the desire to deprescribe among participants. The study included 535 participants, out of which 388 were analyzed, with 233 (43.6%) being women. The majority, 77% (n = 412), were aged between 65 and 74 years. Out of the total, 205 patients (38.4%) had one to two medical conditions and were prescribed between one and five medications. The participants showed a high willingness to deprescribe, and this willingness was inversely associated with sex (p = 0.15), age (p = 0.15), and polypharmacy (p = 0.044). Many older patients visiting geriatric clinics in primary care settings in Kuwait were receptive to the concept of deprescribing medications, particularly if advised by their doctor. Nevertheless, it was observed that male patients, individuals on more than 5 medications, and older age groups showed lower willingness to deprescribe.
    MeSH terms: Deprescriptions*; Aged; Aged, 80 and over; Female; Humans; Kuwait; Male; Surveys and Questionnaires; Polypharmacy*
  4. Hlayel M, Mahdin H, Hayajneh M, AlDaajeh SH, Yaacob SS, Rejab MM
    PLoS One, 2024;19(12):e0314691.
    PMID: 39700470 DOI: 10.1371/journal.pone.0314691
    The rapid development of Digital Twin (DT) technology has underlined challenges in resource-constrained mobile devices, especially in the application of extended realities (XR), which includes Augmented Reality (AR) and Virtual Reality (VR). These challenges lead to computational inefficiencies that negatively impact user experience when dealing with sizeable 3D model assets. This article applies multiple lossless compression algorithms to improve the efficiency of digital twin asset delivery in Unity's AssetBundle and Addressable asset management frameworks. In this study, an optimal model will be obtained that reduces both bundle size and time required in visualization, simultaneously reducing CPU and RAM usage on mobile devices. This study has assessed compression methods, such as LZ4, LZMA, Brotli, Fast LZ, and 7-Zip, among others, for their influence on AR performance. This study also creates mathematical models for predicting resource utilization, like RAM and CPU time, required by AR mobile applications. Experimental results show a detailed comparison among these compression algorithms, which can give insights and help choose the best method according to the compression ratio, decompression speed, and resource usage. It finally leads to more efficient implementations of AR digital twins on resource-constrained mobile platforms with greater flexibility in development and a better end-user experience. Our results show that LZ4 and Fast LZ perform best in speed and resource efficiency, especially with RAM caching. At the same time, 7-Zip/LZMA achieves the highest compression ratios at the cost of slower loading. Brotli emerged as a strong option for web-based AR/VR content, striking a balance between compression efficiency and decompression speed, outperforming Gzip in WebGL contexts. The Addressable Asset system with LZ4 offers the most efficient balance for real-time AR applications. This study will deliver practical guidance on optimal compression method selection to improve user experience and scalability for AR digital twin implementations.
    MeSH terms: Algorithms*; Humans; Models, Theoretical; Mobile Applications
  5. Wang R, Raman A
    Nurse Educ Pract, 2024 Dec 16;82:104238.
    PMID: 39700839 DOI: 10.1016/j.nepr.2024.104238
    AIM: The study aimed to comprehensively analyze and evaluate the effects of blended learning in nursing education.

    BACKGROUND: Blended learning addresses significant challenges in nursing education by offering flexible and adaptive learning environments that support the development of both theoretical knowledge and practical skills. Its importance grew post-COVID-19. Although some reviews have synthesized its effects in nursing education, they often focused on a limited scope and studies, leaving gaps in understanding its broader impact.

    DESIGN: Systematic literature review.

    METHODS: We employed Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines to search for literature published in PubMed, CINAHL, Web of Science, EMBASE, ERIC, Scopus and Cochrane Library from 2000 to June 26, 2024. Two researchers independently screened each article for eligibility and extracted the data.

    RESULTS: This review included 54 studies and found that blended learning positively influenced nursing students in five key areas: academic performance and skill development, learning engagement and motivation, self-management in learning, psychological and emotional well-being, learning experience and satisfaction. These were captured in a visual model showing the comprehensive impact of blended learning. Critical research gaps were identified, including a lack of randomized controlled trials and limited studies on mental health and clinical thinking abilities.

    CONCLUSIONS: The results of the study showed that the effects of blended learning in nursing education were positive and multi-dimensional. Despite some inconsistencies in findings, blended learning was shown to offer significant advantages over traditional learning, though further research is required to address areas of uncertainty.

  6. Quar TK, Lim YF, Rashid MFN, Chu SY, Chong FY
    J Am Acad Audiol, 2024 Jul;35(7-08):204-213.
    PMID: 39701149 DOI: 10.1055/s-0044-1791214
    OBJECTIVE:  Many hearing aid manufacturers have developed application programs that allow audiologists to fine-tune or adjust their patients' hearing aids remotely. This study aims to explore audiologists' experiences in using the remote hearing aid adjustment in Malaysia.

    METHOD:  This is a qualitative research design where focus group discussions among audiologists were held. The interview data were analyzed using reflexive thematic analysis to identify patterns and reconstruct meaning.

    STUDY SAMPLE:  Ten audiologists with at least 1 year of work experience in providing remote hearing aid adjustment services to adult patients were recruited.

    RESULTS:  Even though there has been an increased utilization of remote fine-tuning among audiologists in Malaysia due to the coronavirus disease 2019 pandemic, its use among audiologists remains limited at present. Five thematic areas were identified: "Adoption of Practice," "Challenges and Limitations," "Benefits and Opportunities," "Satisfaction," and "Recommendations for Improvement." The TedAP challenges (T-technical challenges: e-ease of use, d-digital competency; A-abuse of system, and P-physical examinations) have been identified under the "Challenges and Limitations" theme. Suggestions for improvement include integrating the applications and video calls on a common platform, assigning a "standby" trained audiologist, and incorporating remote fine-tuning into audiology education.

    CONCLUSION:  Challenges and limitations associated with the provision of tele-audiology have been documented based on the views and experiences of audiologists in Malaysia. Despite the barriers, audiologists acknowledged the benefits associated with remote systems and recommendations were provided to improve the services. The results of this study could contribute to the increase in the use of tele-audiology in Malaysia. This would be instrumental in developing further the audiology profession in terms of the adoption of tele-audiology and telehealth in Malaysia.

    MeSH terms: Adult; Attitude of Health Personnel; Female; Hearing Aids*; Humans; Malaysia; Male; Correction of Hearing Impairment/methods; Focus Groups; Telemedicine*; Hearing Loss/rehabilitation; Hearing Loss/therapy; Qualitative Research*; Audiologists*
  7. Ye DN, Wang LY, Fei WT, Lin Q, Yan LY, Yang BR, et al.
    Zhongguo Zhong Yao Za Zhi, 2024 Sep;49(18):5095-5101.
    PMID: 39701690 DOI: 10.19540/j.cnki.cjcmm.20240611.401
    Tongkat Ali(Eurycoma longifolia Jack.) is a medicinal plant of Simaroubaceae in Southeast Asia, with centuries of medicinal history. Rich clinical and pharmacological research results have been attained for this plant, which demonstrates diverse and definite effects and high safety, showcasing advantages and broad market prospects to be introduced as a new medicinal plant. Currently, E. longifolia is cultivated in Hainan and Guangdong in China. However, it has not been utilized for the medicinal purpose in China, and the systematic study on its traditional Chinese medicine(TCM) properties remains to be carried out. This paper comprehensively reviews and analyzes the application history, clinical trials, biological activities, chemical composition, and safety of Tongkat Ali and probes into its TCM properties. The results suggest that Tongkat Ali is sweet, bitter, pungent, and plain, with tropism to the kidney, spleen, and liver meridians. Tongkat Ali has the main functions of tonifying kidney and replenishing essence, invigorating spleen and replenishing Qi, and soothing liver and relieving depression. The clinical applications of Tongkat Ali encompass kidney essence deficiency, sex apathy, spleen Qi deficiency, fatigue, liver depression and Qi stagnation. The recommended dosage and usage of this medicine are 2-10 g daily and decoction with water, respectively. This study aims to facilitate the compatible use and product development of Tongkat Ali while providing reference for introducing it as a new TCM resource and studying the TCM properties of ginseng plants abroad.
    MeSH terms: Animals; China; Panax/chemistry; Humans; Medicine, Chinese Traditional
  8. Pansiritanachot W, Riyapan S, Shin SD, Chantanakomes J, Thirawattanasoot N, Rangabpai W, et al.
    Int J Emerg Med, 2024 Dec 20;17(1):193.
    PMID: 39701949 DOI: 10.1186/s12245-024-00787-y
    BACKGROUND: Evidence regarding the effect of time to neurosurgical and neuroradiological intervention on outcomes in traumatic brain injury (TBI) across Asia-Pacific region is limited. This study evaluates the quality of care and outcomes for TBI patients undergoing neurosurgical and neuroradiological procedures at different timings.

    METHODS: Adult TBI patients who received any neurosurgical or neuroradiological interventions during the year 2015-2022 in the Pan-Asian Trauma Outcome Study database were analyzed. The time to intervention, as the main exposure, was classified into three groups (Early, Intermediate, and Delayed) using Restricted Cubic Spline (RCS) analysis. The outcomes were in-hospital mortality and unfavorable neurological outcomes. W score was utilized to compare the quality of care among exposure groups. Multivariable logistic regression analysis and interaction analysis were performed to identify the association between the exposure groups and outcomes, reported as adjusted odds ratios (AOR) with 95% confidence intervals (CI).

    RESULTS: A total of 1,780 patients were included. From the RCS analysis, patients were classified into three groups according to time to intervention: Early ( 4.1 h). According to the time to intervention, W score was - 8.6 in the early group, -1.1 in the intermediate group, and + 0.4 in the delayed group. Patients receiving intermediate and delayed intervention showed significantly lower mortality (AOR 0.64, 95% CI 0.47-0.86 and AOR 0.66, 95%CI 0.48-0.90, respectively).

    CONCLUSION: Early neurosurgical and neuroradiological interventions in TBI patients in the Asia-Pacific region were associated with lower quality of care and higher mortality. The quality of care should be focused and improved during the early hours of TBI.

  9. Supramaniam P, Beh YS, Junus S, Devesahayam PR
    BMC Public Health, 2024 Dec 19;24(1):3542.
    PMID: 39702047 DOI: 10.1186/s12889-024-21056-w
    BACKGROUND: Mobile health applications (mHealth apps) offer potential benefits for improving diabetes management, such as better glucose monitoring and patient engagement, but their widespread adoption faces challenges, including privacy concerns and user adherence. This research investigates mHealth app usage among patients living with diabetes in Kinta District, Perak, exploring experiences, challenges and patient perceptions regarding diabetes management using mHealth apps.

    METHODOLOGY: A cross-sectional community survey was conducted in September till November 2020 across nine government health clinics focusing on diabetes mellitus (Type 1 or Type 2) patients, aged 18 years and older, receiving Diabetes Medication Adherence Counseling (DMTAC) services and able to use smart devices. A self-developed questionnaire with four sections was used to gather demographic information, explore mHealth apps usage and understand both users and non-users' experiences and perceptions. The questionnaire was tested through cognitive debriefing, translated into Malay, pre-tested and finalized by the expert committee. The questionnaire was digitally implemented using Google® Form and QR code. After obtaining informed consent, data collection was performed by the trained DMTAC pharmacists. Statistical analyses involved descriptive and inferential analyses.

    RESULTS: The study analyzed the engagement of 295 patients living with diabetes with mHealth apps. Females (54.9%), of Malay ethnicity (58.3%) and with a mean age of 53.8 years (SD: 12.38) constituted the majority. Diabetes duration had a median of 6 years (IQR: 3.0, 10.0) with prevalent comorbidities like hypertension (58.0%) and dyslipidemia (42.7%). Most patients were employed (44.7%) and their primary source of diabetes management information was through healthcare providers (92.5%). Despite the high app use for social interaction, only 13.6% used mHealth apps for disease management. Users were influenced by social media (65.0%) and favored for wellness apps and disease monitoring. Users perceived the mHealth app as useful (97.5%), yet faced challenges over the app initiation, charges and data security. Non-users cited lack of awareness (70.2%), struggled with app startup (22.4%) and preference for conventional healthcare visits (22.0%). In multivariable analysis, longer diabetes duration reduced mHealth app usage (p = 0.046), while multimorbidity increased the likelihood (p = 0.001). Awareness of the availability of health apps significantly influenced the usage of mHealth apps (p 

    MeSH terms: Adult; Aged; Cross-Sectional Studies; Diabetes Mellitus/therapy; Diabetes Mellitus, Type 2/psychology; Diabetes Mellitus, Type 2/therapy; Female; Humans; Malaysia; Male; Middle Aged; Surveys and Questionnaires; Telemedicine*; Young Adult
  10. Leyva-Grado VH, Promeneur D, Agans KN, Lazaro GG, Borisevich V, Deer DJ, et al.
    NPJ Vaccines, 2024 Dec 19;9(1):244.
    PMID: 39702562 DOI: 10.1038/s41541-024-01036-2
    The limited but recurrent outbreaks of the zoonotic Nipah virus (NiV) infection in humans, its high fatality rate, and the potential virus transmission from human to human make NiV a concerning threat with pandemic potential. There are no licensed vaccines to prevent infection and disease. A recombinant Hendra virus soluble G glycoprotein vaccine (HeV-sG-V) candidate was recently tested in a Phase I clinical trial. Because NiV outbreaks are sporadic, and with a few cases, licensing will likely require an alternate regulatory licensing pathway. Therefore, determining a reliable vaccine correlate of protection (CoP) will be critical. We assessed the immune responses elicited by HeV-sG-V in African Green monkeys and its relationship with protection from a NiV challenge. Data revealed values of specific binding and neutralizing antibody titers that predicted survival and allowed us to establish a mechanistic CoP for NiV Bangladesh and Malaysia strains.
  11. Govindasamy N, Barman M, Salleh N, Giribabu N, Shahzad H
    PMID: 39702602 DOI: 10.1007/s00210-024-03700-9
    Rheumatoid arthritis (RA) can cause blood pressure (BP) elevation in estrogen-deficient, post-menopausal women; however, the underlying mechanisms are not well understood. In this study, the aortic involvement and its underlying mechanisms that contribute to the BP elevation in estrogen-deficient, RA condition were identified. Ovariectomy was performed to create a state of estrogen deficiency and RA was then induced in ovariectomized rats by using incomplete Freund's adjuvant and immune-mediated collagen type-II. Ovariectomized, RA-induced rats (Ovx + RA) were given either 17β-estradiol, baricitinib, or losartan. Direct blood pressure (BP) monitoring was made via cannulation of the carotid artery. Rats were then sacrificed and the aorta was harvested followed by H&E and Picrosirius staining to evaluate histological changes and collagen deposition. Oxidative stress, inflammation, apoptosis, growth, and fibrosis levels in the aorta were assessed by using molecular biological techniques. Mean arterial pressure (MAP) was significantly elevated in Ovx + RA rats when compared to sham and Ovx rats (p 
  12. Leonhard SE, Papri N, Querol L, Rinaldi S, Shahrizaila N, Jacobs BC
    Nat Rev Dis Primers, 2024 Dec 19;10(1):97.
    PMID: 39702645 DOI: 10.1038/s41572-024-00580-4
    Guillain-Barré syndrome (GBS) is a rare immune-mediated polyradiculoneuropathy. Patients typically develop rapidly progressive weakness and sensory deficits that can result in complete paralysis requiring mechanical ventilation. GBS is usually a monophasic disease in which an aberrant immune response to an infection or other trigger damages the peripheral nerves. For example, in patients with preceding Campylobacter jejuni infection, molecular mimicry causes a cross-reactive antibody response to nerve gangliosides. Diagnosis is based on clinical features, supported by cerebrospinal fluid analysis and nerve conduction studies. Effective treatments include plasma exchange and intravenous immunoglobulins. However, ~20% of patients who received treatment are unable to walk after 6 months and ~5% die as a consequence of GBS. Important knowledge gaps in GBS include its pathogenesis, especially after viral infections. In addition, there is a lack of specific biomarkers to improve the diagnosis, monitor the disease activity, and predict the clinical course and outcome of GBS. Major challenges for the future include finding more effective and personalized treatments, which are affordable in low-income and middle-income countries, and preparation for outbreaks of infections as potential triggers for GBS.
    MeSH terms: Humans; Plasma Exchange/methods; Campylobacter jejuni/pathogenicity; Immunoglobulins, Intravenous/therapeutic use
  13. Kalam N, Balasubramaniam VRMT
    Influenza Other Respir Viruses, 2024 Dec;18(12):e70064.
    PMID: 39702696 DOI: 10.1111/irv.70064
    Enterovirus-D68 (EV-D68) was first identified in 1962 in pediatric patients with acute respiratory conditions in California, USA (US). From the 1970s to 2005, EV-D68 was underestimated due to limited data and serotyping methods. In 2014, the United States experienced outbreaks of acute flaccid myelitis (AFM) in children EV-D68 positive. WIN-like compounds (pleconaril, pocapavir, and vapendavir) bind to the virus capsid and have been tested against various enteroviruses (EVs) in clinical trials. However, these compounds encountered issues with resistance and adverse effects, which impeded their approval by the Food and Drug Administration (FDA). Presently, the medical field lacks FDA-approved antiviral treatments or vaccines for EV-D68. Ongoing research efforts are dedicated to identifying viable therapeutics to address EV-D68 infections. This review explores the current advancements in antiviral therapies and potential therapeutics to mitigate the significant impact of EV-D68 infection control.
    MeSH terms: Humans; United States/epidemiology
  14. Mohamed SM, Abou-Ghadir OMF, El-Mokhtar MA, Aboraia AS, Abdel-Moty SG, Abdel-Aal AM
    Chem Biol Drug Des, 2024 Dec;104(6):e70029.
    PMID: 39702898 DOI: 10.1111/cbdd.70029
    The immune system is essential for the defense against infections and is critically implicated in various disorders, including immunodeficiency, autoimmunity, inflammation and cancer. The current study includes a new design of palmitoylated derivatives of thioglycolic acids (PTGAs) capable of triggering innate immune responses. The new series were accessible through a three-step synthetic route, including N-palmitoylation, Claisen-Schmidt condensation and thia-Michael addition. Their structures were elucidated using different 1D and 2D NMR spectroscopic techniques and their purity was confirmed by elemental analysis. The most active PTGAs induced a 12-26-fold increase in the expression of TNF-α and IL-1β mRNA and triggered a marked release of NO in isolated macrophages. These levels were comparable to the responses elicited by heat-killed E. coli and S. aureus. The position of the palmitamide chain and aryl substitution had a significant effect on the TNF-α and IL-1β mRNA expression and NO release. Simulations of molecular dockings showed that the new PTGA derivatives occupy the same TLR2/TLR6 heterodimer active binding site of the microbial diacylated lipoproteins. The new immunomodulators may have a profound impact on various clinical disorders associated with dysfunctional innate immunity.
    MeSH terms: Animals; Escherichia coli/drug effects; Immunologic Factors/chemical synthesis; Immunologic Factors/pharmacology; Immunologic Factors/chemistry; Macrophages/drug effects; Macrophages/metabolism; Nitric Oxide/metabolism; Drug Design*; Toll-Like Receptor 2/metabolism; Mice; Lipoylation/drug effects; Molecular Docking Simulation
  15. Yang D, Zhang Z, Zhao L, Sui W, Li Y, Zhou Y, et al.
    Cell Biochem Funct, 2024 Dec;42(8):e70032.
    PMID: 39702946 DOI: 10.1002/cbf.70032
    Phospholipase A2 receptor 1 (PLA2R1) exists important role in membranous nephropathy. In this study, we evaluate a PLA2R1 in a middle-aged rat model of renal function repair to further investigate the molecular mechanisms of membranous nephropathy. We analyzed the PLA2R1 knockout (KO) model and PLA2R1 knock in (KI) model in rats, extending the time to 85 weeks of age. Urinary biochemical indicators were detected using a fully automated biochemical analyzer. The complement C3, IgG, and Nephrin were detected using the immunofluorescence method. Western blot was used to detect the expression levels of complement C3, IgA and PLA2R1 in middle-aged models. The KO model continues to display glomerular proteinuria, complement C3 aggregation, and IgA and IgG deposition. Comparing with the KO model, the deposition of complement C3 and IgA in the glomerulus of the KI chimeric model still exists and IgG expression weakened. Inserting humanized PLA2R1 into rats can continuously repair partial renal function and reduce proteinuria, which will help investigate the pathogenesis of membranous nephropathy and complement activation signaling pathways.
    MeSH terms: Animals; Disease Models, Animal*; Humans; Immunoglobulin A/metabolism; Immunoglobulin G; Kidney/metabolism; Kidney/pathology; Male; Membrane Proteins; Proteinuria/metabolism; Rats; Gene Knock-In Techniques
  16. Choon LK, Khiruddin AI
    PMID: 39703119 DOI: 10.1177/10806032241304734
    The Solanaceae plant family (Solanum), such as capsicum, eggplant, potato, and tomato, is made up of common plants consumed by humans. Despite having medicinal benefits, these plants can produce toxic alkaloid compounds. We report 2 cases of uncommon Solanum erianthum poisoning after consuming the berries as a meal. Both patients presented with slurring of speech, which is easily mistaken for a stroke. Symptoms developed 19 h after consuming the wild berries and resolved spontaneously 12 h after onset. Both patients recovered with no neurologic deficit. No antidote was given in either case. The Solanaceae plant family is a common group of plants consumed by humans and is considered one of the largest genera. The phytochemical profile showed that the leaves and berries contain toxic glycoalkaloid levels, which can cause gastrointestinal and neurologic symptoms in poisoning cases. Reports have shown that the toxic glycoalkaloids can inhibit the cholinesterase enzyme, thus producing cholinergic effects. Theoretically, atropine is the choice of antidote, but no case report of atropine use has been documented. Treatment of S erianthum poisoning remains supportive care. These cases highlight the rare occurrence of poisoning from S erianthum and emphasize the necessity for considering toxicologic causes in nonlocalizing neurologic symptoms.
  17. Geurts J, Singh-Povel C, Lee ST, Sekartini R, Poh BK, Rojroongwasinkul N, et al.
    Public Health Nutr, 2024 Dec 20;27(1):e261.
    PMID: 39703175 DOI: 10.1017/S1368980024001332
    OBJECTIVE: To describe the economic, lifestyle and nutritional impact of the COVID-19 pandemic on parents, guardians and children in Malaysia, Indonesia, Thailand and Vietnam.

    DESIGN: Data from the SEANUTS II cohort were used. Questionnaires, including a COVID-19 questionnaire, were used to study the impact of the pandemic on parents/guardians and their children with respect to work status, household expenditures and children's dietary intake and lifestyle behaviours.

    SETTING: Data were collected in Malaysia, Indonesia, Thailand and Vietnam between May 2019 and April 2021.

    PARTICIPANTS: In total, 9203 children, aged 0·5-12·9 years, including their parents/guardians.

    RESULTS: Children and their families were significantly affected by the pandemic. Although the impact of lockdown measures on children's food intake has been relatively mild in all countries, food security was negatively impacted, especially in Indonesia. Surprisingly, in Malaysia, lockdown resulted in overall healthier dietary patterns with more basic food groups and less discretionary foods. Consumption of milk/dairy products, however, decreased. In the other countries, intake of most food groups did not change much during lockdown for households based on self-reporting. Only in rural Thailand, some marginal decreases in food intakes during lockdown persisted after lockdown. Physical activity of children, monthly household income and job security of the parents/guardians were negatively affected in all countries due to the pandemic.

    CONCLUSION: The COVID-19 pandemic has significantly impacted societies in South-East Asia. To counteract negative effects, economic measures should be combined with strategies to promote physical activity and eating nutrient-adequate diets to increase resilience of the population.

    MeSH terms: Adult; Child; Child, Preschool; Diet/statistics & numerical data; Female; Humans; Indonesia/epidemiology; Infant; Life Style; Malaysia/epidemiology; Male; Parents/psychology; Surveys and Questionnaires; Thailand/epidemiology; Vietnam/epidemiology; Cohort Studies; Exercise; Pandemics
  18. Conway RB, Snell-Bergeon J, Honda-Kohmo K, Peddi AK, Isa SB, Sulong S, et al.
    J Endocr Soc, 2024 Nov 26;9(1):bvae210.
    PMID: 39703363 DOI: 10.1210/jendso/bvae210
    Globally, nearly 9 million people are living with type 1 diabetes (T1D). Although the incidence of T1D is not affected by socioeconomic status, the development of complications and limited access to modern therapy is overrepresented in vulnerable populations. Diabetes technology, specifically continuous glucose monitoring and automated insulin delivery systems, are considered the gold standard for management of T1D, yet access to these technologies varies widely across countries and regions, and varies widely even within high-income countries. This review focuses on disparities in diabetes technology use among adolescents and young adults with T1D, barriers to access and use, and summarizes common themes emerging across countries and regions. We conducted a survey among medical technology manufacturers and physicians in various countries across diverse geographical regions and performed extensive literature searches. Across all countries and regions, economic barriers stand out as the largest and most common barriers, either preventing market penetrance of technology into a country or limiting its access to the individual with diabetes due to high out of pocket costs. Other common barriers include structural or accessibility barriers, such as stringent eligibility requirements by insurance providers, regardless of whether the insurance was private or government-based, and provider/individual level barriers. Based on the evidence presented, we suggest the need for a joint effort involving governments, private health insurers, technology manufacturers, and healthcare providers to address the global disparities of diabetic technology utilization and ensure equitable access for all individuals living with T1D worldwide.
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