METHODS: Exosomes were isolated by ultracentrifugation and characterized using nanoparticle tracking analysis (NTA), scanning electron microscopy (SEM), and Western blot. The effect of exosomes in modulating monocyte phenotypes as well as cytokine secretion were further assessed in a co-culture condition using flow cytometry and ELISA accordingly.
RESULTS: Exosomes were identified as spherical particles with a size distribution ranging from 30 nm to 150 nm. These nanoparticles intensely expressed exosome protein markers including CD9, CD63, CD81, and HSP70. The expression of HLA-DR, CD14, and CD11b on monocytes decreased in the presence of exosomes after 24 h of incubation, regardless of the dose. Exosomes significantly induced the release of anti-inflammatory cytokines IL-1Ra in a time- and dose-dependent manner, while TNF-α secretion remains unchanged regardless of the presence or absence of exosomes.
CONCLUSION: This study highlights the immunoregulatory role of exosomes on monocytes, emphasizing the need for further studies into the underlying mechanism.
DESIGN: Data from the SEANUTS II cohort were used. Questionnaires, including a COVID-19 questionnaire, were used to study the impact of the pandemic on parents/guardians and their children with respect to work status, household expenditures and children's dietary intake and lifestyle behaviours.
SETTING: Data were collected in Malaysia, Indonesia, Thailand and Vietnam between May 2019 and April 2021.
PARTICIPANTS: In total, 9203 children, aged 0·5-12·9 years, including their parents/guardians.
RESULTS: Children and their families were significantly affected by the pandemic. Although the impact of lockdown measures on children's food intake has been relatively mild in all countries, food security was negatively impacted, especially in Indonesia. Surprisingly, in Malaysia, lockdown resulted in overall healthier dietary patterns with more basic food groups and less discretionary foods. Consumption of milk/dairy products, however, decreased. In the other countries, intake of most food groups did not change much during lockdown for households based on self-reporting. Only in rural Thailand, some marginal decreases in food intakes during lockdown persisted after lockdown. Physical activity of children, monthly household income and job security of the parents/guardians were negatively affected in all countries due to the pandemic.
CONCLUSION: The COVID-19 pandemic has significantly impacted societies in South-East Asia. To counteract negative effects, economic measures should be combined with strategies to promote physical activity and eating nutrient-adequate diets to increase resilience of the population.
METHODS: A voluntary sample of 600 participants, aged 15 to 29, was recruited. Data were analyzed using Partial Least Squares-Structural Equation Modeling (PLS-SEM) to examine the relationships between variables.
RESULTS: The analysis revealed that all three intrinsic needs-competence, autonomy, and relatedness-along with social media algorithms and source credibility, positively correlated with fitness-related health information use behaviors among youth. Additionally, social media algorithms moderated the relationship between the need for relatedness and fitness-related health information behavior.
DISCUSSION: These findings provide new insights into developing health communication strategies on social media, particularly targeted toward the youth demographic, enhancing our understanding of effective health information dissemination in digital environments.
METHODS: Forty-eight patients with hand fractures were recruited over a period of 15 months. Twenty-one patients had undergone fracture fixation under GA, whereas another 27 patients had the surgery under WALANT. Parameters including vital signs (heart rate and systolic blood pressure), surgery duration, estimated blood loss, visual analog scale, and quick disabilities of the arm, shoulder, and hand (QuickDASH) questionnaire were evaluated.
RESULTS: Wide-awake local anesthesia no tourniquet group had significantly higher systolic blood pressure values and higher estimated blood less than GA group. Wide-awake local anesthesia no tourniquet group also documented shorter surgical time compared with GA group, but the difference was not significant. In terms of clinical outcomes, WALANT group recorded significantly lower visual analog scale score at 2 hours and 2 weeks postsurgery. However, WALANT has a comparable QuickDASH score with GA group except at 3 months postsurgery.
CONCLUSIONS: Wide-awake local anesthesia no tourniquet surgery for hand fracture fixation provides a similar analgesic effect and comparable QuickDASH score except at 3 months postsurgery compared with GA.
TYPE OF STUDY/LEVEL OF EVIDENCE: Therapeutic IIb.
METHODS: We conducted 20 in-depth interviews with stakeholders, including government officials, community members, and representatives of non-governmental organisations (NGOs), using a qualitative methodology. The data were analysed using thematic analysis to identify key themes surrounding public participation, policy challenges, and successful community-led flood mitigation initiatives.
RESULTS: The findings highlight the critical role of public involvement in improving flood preparedness and resilience. Communities that actively participated in mitigation efforts, such as early warning systems and nature-based solutions, demonstrated enhanced resilience. However, significant challenges remain, including inadequate funding, outdated infrastructure, and bureaucratic delays. Public awareness and education on flood preparedness, especially in rural areas, were found to be insufficient, hindering the effectiveness of flood management strategies.
CONCLUSIONS: The study concludes that while CBFM initiatives in Malaysia show promise, their success depends on stronger policy enforcement, increased public engagement, and sustained investments in both green and grey infrastructure. Greater collaboration between local communities, NGOs, and government agencies is essential for improving flood risk management and building long-term resilience, particularly in the face of increasing climate-driven disasters.