Browse publications by year: 2024

  1. Zhong Yi K, Selvaratnam V
    Cureus, 2024 Oct;16(10):e71560.
    PMID: 39544611 DOI: 10.7759/cureus.71560
    Femoral impaction bone grafting is a crucial technique in revision hip arthroplasty, addressing bone loss and ensuring implant stability. The choice between cemented and uncemented stems significantly influences the outcomes and long-term success of the procedure. This systematic review aims to compare the clinical outcomes of cemented versus uncemented stems in femoral impaction bone grafting. A comprehensive search of PubMed, MEDLINE Complete, and the Cochrane Library databases was conducted in accordance with Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines. Studies evaluating the outcomes of femoral impaction bone grafting with cemented or uncemented stems were included. The primary outcome measured was the rate of loosening of the femoral component, while secondary outcomes included the incidence of complications such as dislocation, infection, fractures, overall patient mortality, and cardiopulmonary diseases. The literature search yielded 78 articles, with 36 meeting the inclusion criteria. These included one randomized controlled trial, 16 cohort studies (10 retrospective and six prospective), and 15 case series. Most surgeries were revision procedures, with aseptic loosening being the most common indication. For the cemented technique, 1,588 hips were analyzed, with 8.00% experiencing aseptic loosening, 3.53% dislocation, 3.87% infection, 37.33% mortality, 7.57% fractures, and 1.13% cardiopulmonary complications. For the uncemented technique, 464 hips were analyzed, with 1.72% aseptic loosening, 4.74% dislocation, 1.5% infection, 38.47% mortality, 7.76% fractures, and 0.65% cardiopulmonary complications. This systematic review highlights that both cemented and uncemented techniques for femoral impaction bone grafting offer unique benefits and challenges, with the choice depending on patient-specific factors. The uncemented technique, with a lower risk of femoral component loosening, may be better suited for younger, active patients with good bone quality, despite a slightly higher risk of fractures and dislocations. In contrast, the cemented technique, offering immediate stability, is more appropriate for elderly patients with compromised bone quality but carries a higher risk of loosening and cardiopulmonary complications. The decision should be tailored to the patient's clinical profile, including age, bone quality, comorbidities, and the surgeon's expertise.
  2. Ait Bouabdallah I, Adjal F, Zaabar A, Benchikh A, Guerniche D, Ait Ramdane-Terbouche C, et al.
    RSC Adv, 2024 Nov 11;14(49):36423-36436.
    PMID: 39545168 DOI: 10.1039/d4ra06477a
    The search for sustainable, cost-effective and environmentally friendly corrosion inhibitors for hydrochloric acid (HCl) solution in industrial applications has garnered increasing interest in plant extracts and their refined metabolites. In this research, Cleome arabica L. (CA) extract, found in the Algerian Sahara, was considered due to its low cost compared to other studied plants and higher content of active compounds, thereby emerging as a promising candidate and offering the potential to promote a circular economy model. This study assessed the effectiveness of CA extract as a green corrosion inhibitor for AISI 1045 carbon steel in 0.5 M HCl solution and highlighted its potential to advance the field of green corrosion inhibitors. ATR-FTIR and LC-ESI-MS/MS analyses revealed the presence of significant organic compounds, including coumaric acid (74.58%), 4-methoxybenzoic acid (12.53%), and kaempferol (8.05%), which contributed to the corrosion inhibition. The inhibitory effectiveness of the CA extract was evaluated at five concentrations, ranging from 0.125 to 1 g L-1, using weight loss measurements, potentiodynamic polarization (PDP) and electrochemical impedance spectroscopy (EIS). The highest inhibition efficiency (η = 94.45%) was observed at a CA extract concentration of 1 g L-1 after 196 hours of immersion in 0.5 M HCl. Thermodynamic analysis using the Langmuir adsorption isotherm yielded a ΔG ads value of -24.737 kJ mol-1, indicating the spontaneous adsorption of CA molecules onto the AISI 1045 surfaces, forming a protective layer, which was confirmed by SEM/EDX analysis. Density functional theory (DFT) calculations showed a significant correlation with the experimental data, confirming that CA extract is a highly efficient and environmentally friendly corrosion inhibitor.
  3. Pratama DAO, Fernanda A, Raissa R, Permata FS, Nordin ML
    Open Vet J, 2024 Oct;14(10):2678-2686.
    PMID: 39545186 DOI: 10.5455/OVJ.2024.v14.i10.17
    BACKGROUND: Mammary gland carcinoma is a malignant type of cancer that occurs in mammae tissue. Dimethylbenzene (α) anthracene (DMBA) is a carcinogenic agent that causes mammary cancer by damaging cellular DNA. Flavonoids found in the black soybean (Glycine max L. Merr) exhibit anti-carcinogenic effects.

    AIM: This study evaluated the anticarcinogenic effects of black soybean extract.

    METHODS: The activity of flavonoid compounds in black soybean was determined in silico. Five groups of rats, four in each group, were established, consisting of a negative control, a positive control, and three treatment groups. Treatment included black soybean extract administration (i.e., T1 = 200, T2 = 400, and T3 = 800 mg of black soybean extract/kg body weight for 10 days). The observed parameters included the immunohistochemical analysis of Breast Cancer 1(BRCA1) and TNF-α.

    RESULTS: Based on an in silico study, compounds from black soybeans are non-toxic. Functional annotation analysis revealed that most of the target proteins have a role in biological processes associated with cancer development. An in vivo analysis using an animal mammae cancer model indicated that black soybean extracts inhibited mammae cancer progression by attenuating TNF-α and BRCA1 expression.

    CONCLUSION: The most effective dosage of black soybean extract was 200 mg/kg body weight. An increase in BRCA1 and TNF-α expression may be related to the effects of catechin, daidzein, genistein, and glycitein, which are present in black soybeans.

    MeSH terms: Animals; Computer Simulation; Female; Mammary Neoplasms, Animal/chemically induced; Rats, Sprague-Dawley; BRCA1 Protein/genetics; BRCA1 Protein/metabolism; Rats
  4. Saniasiaya J, van der Meer G, Toll EC
    J Laryngol Otol, 2024 Nov 15.
    PMID: 39545317 DOI: 10.1017/S0022215124001038
    OBJECTIVE: Drooling or saliva spillage has been explored widely among children with neurodevelopmental conditions. Yet, the approach to drooling in an otherwise developmentally normal child remains unexplored, as it is regarded as self-limiting. Nonetheless, drooling beyond age 4 in the awake stage should raise concern.

    METHODS: This narrative review aims to shed light on drooling in developmentally normal children, also known as 'healthy droolers', and the available evidence on its management.

    RESULTS: Most notable factors causing saliva spillage include poor oral-motor control and impaired oral sensation. Delayed saliva acquisition may be an early indicator of developmental or intellectual delay. Drooling impairs both the children's and parents' overall quality of life significantly.

    CONCLUSION: Healthy droolers can be managed by simple behavioural therapy and reassurance.

  5. Leow SS, Khoo JS, Lee WK, Hoh CC, Fairus S, Sambanthamurthi R, et al.
    J Appl Genet, 2024 Dec;65(4):867-895.
    PMID: 38890243 DOI: 10.1007/s13353-024-00880-1
    Water-Soluble Palm Fruit Extract (WSPFE) has been shown to confer anti-diabetic effects in the Nile rat (NR) (Arvicanthis niloticus). Liquid and powder WSPFE both deterred diabetes onset in NRs fed a high-carbohydrate (hiCHO) diet, but the liquid form provided better protection. In this study, NRs were fed either a hiCHO diet or the same diet added with liquid or powder WSPFE. Following feeding of the diets for 8 weeks, random blood glucose levels were measured to categorize NRs as either diabetes-resistant or diabetes-susceptible, based on a cut-off value of 75 mg/dL. Livers were then obtained for Illumina HiSeq 4000 paired end RNA-sequencing (RNA-Seq) and the data were mapped to the reference genome. Consistent with physiological and biochemical parameters, the gene expression data obtained indicated that WSPFE was associated with protection against diabetes. Among hepatic genes upregulated by WSPFE versus controls, were genes related to insulin-like growth factor binding protein, leptin receptor, and processes of hepatic metabolism maintenance, while those downregulated were related to antigen binding, immunoglobulin receptor, inflammation- and cancer-related processes. WSPFE supplementation thus helped inhibit diabetes progression in NRs by increasing insulin sensitivity and reducing both the inflammatory effects of a hiCHO diet and the related DNA-damage compensatory mechanisms contributing to liver disease progression. In addition, the genetic permissiveness of susceptible NRs to develop diabetes was potentially associated with dysregulated compensatory mechanisms involving insulin signaling and oxidative stress over time. Further studies on other NR organs associated with diabetes and its complications are warranted.
    MeSH terms: Animals; Blood Glucose; Insulin Resistance/genetics; Male; Gene Expression Profiling; Rats; Transcriptome/drug effects
  6. Chakravarthi KK, Nelluri V, Reghunadhan D, Sugavasi R
    Ann Afr Med, 2024 Oct 01;23(4):641-648.
    PMID: 39138967 DOI: 10.4103/aam.aam_73_24
    BACKGROUND: The foramen transversarium is a vital anatomical structure found in the cervical vertebrae of the spine. Typically, it serves as a passageway for important neurovascular structures, including the vertebral artery and vein, as well as the vertebral nerve. However, abnormal calcification or ossification of soft tissues in and around this area can lead to various clinical implications. Understanding the presence and implications of abnormal ossified structures in and around the foramen transversarium is crucial for clinicians involved in the diagnosis and management of cervical spine disorders.

    AIMS: Accordingly, this present study was designed to evaluate the abnormal ossified structures anatomically and radiologically within and around the foramen transversarium.

    MATERIALS AND METHODS: This study was conducted on 182 (26 sets of cervical vertebrae) dried human cervical vertebrae obtained from the respective departments of anatomy and on 190 (95 males and 95 females) adult patients who visited the radiology department for neck-related problems such as stiff neck, neck/shoulder pain, dizziness, vertigo, imbalance, visual disturbances, and cognitive impairment.

    RESULTS: Among 182 examined cervical vertebrae, unilateral complete accessory foramen transversarium was found in 23 vertebrae (12.63%), bilateral complete in 19 (10.44%), bilateral incomplete in 6 (3.29%), unilateral complete double in 4 (2.19%), and unilateral complete absence of foramen transversarium in 3 (1.64%). Stenosis due to aberrant osteophytes was noted in 9 vertebrae (4.9%). Out of 190 patients, three males presented with cervical kyphosis, severe spinal canal stenosis, and spinal cord compression due to ossification of the posterior longitudinal ligament and osteophyte complexes at C3-C6, with the most significant compression at C5-C6.

    CONCLUSION: A thorough understanding of abnormal ossifications in and around the foramen transversarium is crucial for the management of cervical spine disorders; imaging modalities such as X-ray, computed tomography, and magnetic resonance imaging are crucial for recognizing and intervening in these cases, which is essential to prevent adverse neurological outcomes associated with vertebral artery involvement.

    MeSH terms: Adult; Aged; Calcinosis/pathology; Female; Humans; Male; Middle Aged; Ossification, Heterotopic/pathology; Radiography; Neck Pain/etiology
  7. Chakravarthi KK, Reghunadhan D
    Ann Afr Med, 2024 Oct 01;23(4):697-703.
    PMID: 39279176 DOI: 10.4103/aam.aam_89_24
    BACKGROUND: Anatomical and developmental variations of ureters and renal pelvis have been observed frequently during routine human cadaveric dissection and surgical practice; however, their coexistence with accessory or aberrant renal arteries is exceptionally rare. Accordingly, this study was designed to evaluate the prevalence of anatomical and developmental abnormalities of ureters and renal pelvis existing with accessory renal arteries in human cadavers.

    MATERIALS AND METHODS: This study was carried out on 50 human cadavers including dissected specimens (25 males and 25 females) the kidneys, renal pelvis, and ureters along with their arteries were exposed and the anomalous abnormalities of the renal pelvis and ureters existing with accessory renal arteries were observed. Photographs of the anomalous and developmental variations were taken for proper documentation.

    RESULTS: Among the 50 cadavers studied, unilateral double ureters were found in 5 cadavers (10%), rare bilateral "S-"shaped loop of ureter with quadruple uretic constrictors in the abdominal segment of the ureter was observed in one female cadaver (2%), accessory or aberrant renal arteries were found in 15 cadavers (30%), hydronephrosis involving the renal pelvis and ureters was observed in 9 cadavers (18%). Interestingly, this prevalence was higher among males (28%) compared to females (8%). Moreover, the occurrence of bilateral hydronephrosis of the kidneys, renal pelvis, and ureters was identified in a single male cadaver, representing 2% of the sample. Notably, the prevalence of double ureter, hydronephrosis accompanied by congenital double and triple accessory renal arteries was documented in nine cadavers, accounting for 18% of the cohort.

    CONCLUSION: Anatomical and developmental variations of the ureters, renal pelvis, and renal vasculature, as well as their relationships to surrounding structures, hold clinical significance due to their impact on various surgical procedures, including kidney transplantation, abdominal aorta reconstruction, interventional radiology, and urologic operations. Therefore, identifying these potential developmental variations is essential for effective surgical management to preserve renal function and ensure optimal patient outcomes.

    MeSH terms: Adult; Aged; Cadaver*; Dissection; Female; Humans; Hydronephrosis; Kidney/abnormalities; Kidney/blood supply; Male; Middle Aged; Prevalence
  8. Islam MS, Al Bakky A, Ahmed S, Islam MT, Antu UB, Saikat MSM, et al.
    Food Chem Toxicol, 2024 Nov;193:115005.
    PMID: 39284411 DOI: 10.1016/j.fct.2024.115005
    As a cereal crop, maize ranked third place after wheat and rice in terms of land area coverage for its cultivation, and in Bangladesh, it ranked second place after rice in its production. As the substitution of wheat products, maize has been used widely in baking for human consumption and animal fodder. However, maize grown in this soil around the coal-burning power plant may cause heavy metals uptake that poses a risk to humans. The study was conducted at the maize fields in the Ganges delta floodplain soils of Bangladesh to know the concentration of eight heavy metals (Ni, Cr, Cd, Mn, As, Cu, Zn, and Pb) in soil and maize samples using an inductively coupled plasma mass spectrometer (ICP-MS) and to estimate the risk of heavy metals in maize grains. Mean concentrations of heavy metals (mg/kg) in soil were in decreasing order of Zn (10.12) > Cu (10.02) > Mn (5.48) > Ni (4.95) > Cr (3.72) > As (0.51) > Pb (0.27) > Cd (0.23). The plant tissues showed the descending order of heavy metal concentration as roots > grains > stems > leaves. BCF values for As, Cd, Pb, and Mn in roots were higher than 1.0, indicating considerable accumulation of these elements in maize via roots. Total hazard quotient (ƩTHQ) of heavy metals through maize grain consumption was 3.7E+00 and 3.9E+00 for adults and children, respectively, indicating non-cancer risk to the consumers. Anthropogenic influences contributed to the heavy metals enrichment in the Ganges delta floodplain soils around the thermal plant, and potential risks (non-carcinogenic and carcinogenic) were observed due to the consumption of maize grain cultivated in the study area.
    MeSH terms: Bangladesh; Environmental Monitoring/methods; Food Contamination/analysis; Humans; Power Plants; Soil/chemistry; Risk Assessment
  9. Isaac P, Mutusamy P, Yin LS, Jing Wei Y, Mohd Salleh F, Bin Abu Bakar MAL, et al.
    Microbiol Resour Announc, 2024 Nov 12;13(11):e0029924.
    PMID: 39311881 DOI: 10.1128/mra.00299-24
    We present a complete genome of Serratia marcescens D1_6 isolated from peat swamp forest. The complete genome for the isolate D1_6 was constructed using data from Oxford Nanopore Technologies and Illumina. The genome of D1_6 has a total length of 4,996,151 bp, comprising a chromosome and a plasmid.
  10. Wong CX, Tse HF, Choi EK, Chao TF, Inoue K, Poppe K, et al.
    Nat Rev Cardiol, 2024 Dec;21(12):841-843.
    PMID: 39322762 DOI: 10.1038/s41569-024-01091-1
  11. Loiodice A, Bailly S, Ruckly S, Buetti N, Barbier F, Staiquly Q, et al.
    Clin Microbiol Infect, 2024 Dec;30(12):1559-1568.
    PMID: 39326671 DOI: 10.1016/j.cmi.2024.09.011
    OBJECTIVES: Hospital-acquired bloodstream infections (HA-BSI) in the intensive care unit (ICU) are common life-threatening events. We aimed to investigate the association between early adequate antibiotic therapy and 28-day mortality in ICU patients who survived at least 1 day after the onset of HA-BSI.

    METHODS: We used individual data from a prospective, observational, multicentre, and intercontinental cohort study (Eurobact2). We included patients who were followed for ≥1 day and for whom time-to-appropriate treatment was available. We used an adjusted frailty Cox proportional-hazard model to assess the effect of time-to-treatment-adequacy on 28-day mortality. Infection- and patient-related variables identified as confounders by the Directed Acyclic Graph were used for adjustment. Adequate therapy within 24 hours was used for the primary analysis. Secondary analyses were performed for adequate therapy within 48 and 72 hours and for identified patient subgroups.

    RESULTS: Among the 2418 patients included in 330 centres worldwide, 28-day mortality was 32.8% (n = 402/1226) in patients who were adequately treated within 24 hours after HA-BSI onset and 40% (n = 477/1192) in inadequately treated patients (p 

    MeSH terms: Aged; Aged, 80 and over; Female; Humans; Intensive Care Units*; Male; Middle Aged; Prognosis; Prospective Studies; Proportional Hazards Models
  12. Eskandari A, Leow TC, Rahman MBA, Oslan SN
    Eur Biophys J, 2024 Nov;53(7-8):385-403.
    PMID: 39327310 DOI: 10.1007/s00249-024-01719-7
    Antifreeze proteins (AFPs) have unique features to sustain life in sub-zero environments due to ice recrystallization inhibition (IRI) and thermal hysteresis (TH). AFPs are in demand as agents in cryopreservation, but some antifreeze proteins have low levels of activity. This research aims to improve the cryopreservation activity of an AFPIV. In this in silico study, the helical peptide afp1m from an Antarctic yeast AFP was modeled into a sculpin AFPIV, to replace each of its four α-helices in turn, using various computational tools. Additionally, a new linker between the first two helices of AFPIV was designed, based on a flounder AFPI, to boost the ice interaction activity of the mutants. Bioinformatics tools such as ExPASy Prot-Param, Pep-Wheel, SOPMA, GOR IV, Swiss-Model, Phyre2, MODFOLD, MolPropity, and ProQ were used to validate and analyze the structural and functional properties of the model proteins. Furthermore, to evaluate the AFP/ice interaction, molecular dynamics (MD) simulations were executed for 20, 100, and 500 ns at various temperatures using GROMACS software. The primary, secondary, and 3D modeling analysis showed the best model for a redesigned antifreeze protein (AFP1mb, with afp1m in place of the fourth AFPIV helix) with a QMEAN (Swiss-Model) Z score value of 0.36, a confidence of 99.5%, a coverage score of 22%, and a p value of 0.01. The results of the MD simulations illustrated that AFP1mb had more rigidity and better ice interactions as a potential cryoprotectant than the other models; it also displayed enhanced activity in limiting ice growth at different temperatures.
    MeSH terms: Amino Acid Sequence; Ice; Mutation*; Molecular Dynamics Simulation*
  13. Che Zain MS, Danish M, Shaari K, Fakurazi S
    Daru, 2024 Dec;32(2):689-703.
    PMID: 39340725 DOI: 10.1007/s40199-024-00540-z
    BACKGROUND: Amorphous hydrogel is a strategic wound healing dressings that comprised of water, polymers and excipients with no shape. The dense cross-linked network of polymer is interspersed by the immobilized water component could rehydrate and promote healing in wound tissue.

    OBJECTIVE: In this work, various polysaccharide/gelatin amorphous hydrogels with the impregnation of oil palm leaf derived total flavonoid enriched extract (OPL-TFEE) were fabricated via one-pot synthesis method to provide multiple crosslinking networks.

    METHOD: The bioflavonoids (OPL-TFEE) were derived from Elaeis guineensis leaf using an integrated green extraction and enrichment process. Amorphous hydrogels with good wound healing properties were developed by incorporating 0.3% antioxidant agent into the hybrid polymeric gelling system.

    RESULT: The formulations appeared as a semi-solid dark yellow translucent hydrogel with good spreading and consistency characteristics and satisfying aesthetic properties. The FTIR analysis indicated that the bioflavonoid was compatible with the matrix, and the hydrogels showed porous morphological structures when observed under SEM. Furthermore, the hydrogels possessed shear thinning, pseudoplastic, and elastic properties. Bioflavonoids-impregnated polysaccharide/gelatin hydrogel release 95-98% bioflavonoids within 24 h, while the drug release profile followed the Korsmeyer-Peppas kinetic model. The hydrogels showed antioxidant and wound healing properties with no sign of cytotoxicity.

    CONCLUSION: Overall, the results revealed bioflavonoid-loaded hydrogels exhibited good physicochemical and biological properties, thus could serve as new innovative formulation in the sustainable advancement of wound care product for promoting wound healing.

    MeSH terms: Animals; Antioxidants/pharmacology; Antioxidants/chemistry; Humans; Plant Extracts/pharmacology; Plant Extracts/chemistry; Sapotaceae/chemistry; Drug Liberation*
  14. Borikhonov B, Berdimurodov E, Kholikov T, Nik WBW, Katin KP, Demir M, et al.
    J Mol Model, 2024 Oct 02;30(11):359.
    PMID: 39356293 DOI: 10.1007/s00894-024-06157-y
    CONTEXT: This study addresses the development of sustainable pyridinium ionic liquids (ILs) because of their potential applications in agriculture and pharmaceuticals. Pyridinium-based ILs are known for their low melting points, high thermal stability, and moderate solvation properties. We synthesized three novel pyridinium-based ILs: 1-(2-(isopentyloxy)-2-oxoethyl)pyridin-1-ium chloride, 1-(2-(hexyloxy)-2-oxoethyl)pyridin-1-ium chloride, and 1-(2-(benzyloxy)-2-oxoethyl)pyridin-1-ium chloride. The biological activities of these compounds were evaluated through plant growth promotion, herbicidal, and insecticidal assays. Our results show that the benzyloxy derivative significantly enhances wheat and cucumber growth, whereas the isopentyloxy compound has potent herbicidal effects. Computational methods, including DFT calculations and molecular docking, were applied to understand the structure‒activity relationships (SARs) and mechanisms of action.

    METHODS: The computational techniques involved dispersion-corrected density functional theory (DFT) with the B3LYP functional and the 6-311G** basis set. Grimme's D3 corrections were included to account for dispersion interactions. The calculations were performed via GAMESS-US software. Quantum descriptors of reactivity, such as ionization potential, electron affinity, chemical potential, and electrophilicity index, were derived from the HOMO and LUMO energies. Molecular docking studies were conducted via the CB-Dock server via AutoDock Vina software to predict binding affinities to cancer-related proteins. Petra/Osiris/Molinspiration (POM) analysis was used to predict the drug likeness and other pharmaceutical properties of the synthesized ILs.

    MeSH terms: Herbicides/pharmacology; Herbicides/chemistry; Insecticides/pharmacology; Insecticides/chemistry; Structure-Activity Relationship; Triticum/drug effects; Triticum/growth & development; Molecular Structure; Cucumis sativus/drug effects; Cucumis sativus/growth & development; Molecular Docking Simulation*
  15. Rahman NAAA, Khasri A, Salleh NHM, Jamir MRM
    Environ Sci Pollut Res Int, 2024 Oct;31(49):59398-59415.
    PMID: 39354260 DOI: 10.1007/s11356-024-34948-6
    Excessive use of tetracycline (TC) is alarming owing to its increased detection in water systems. In this study, a photocatalyst was developed to degrade TC using a Ce-N-co-doped AC/TiO2 photocatalyst, denoted as Ce/N-AC/TiO2, prepared using the sol-gel method assisted by microwave radiation, speeding up the synthesis process. Ce/N-AC/TiO2 achieved maximum TC degradation of 93.1% under UV light with optimum sorption system conditions of an initial concentration of 10 mg L-1, pH 7, and 30 ℃, under 120 min. Scavenger experiments revealed that holes and superoxide radicals were the active species influencing the photodegradation process. The TC degradation was appropriately fitted with Langmuir isotherms and a pseudo-second-order (PSO) kinetic model. The change in enthalpy (ΔH) (2.43 kJ mol-1), entropy (ΔS) (0.024 kJ mol-1), and Gibbs free energy (ΔG) (- 4.941 to - 5.802 kJ mol-1) suggested that the adsorption process was spontaneous, favourable, and endothermic. Electrostatic interaction, hydrogen bonding, pore-filling, cationic-π, n-π, and π-π interaction were among the interactions involved between TC and Ce/N-AC/TiO2. Furthermore, Ce/N-AC/TiO2 stability was confirmed through 80% removal efficiency even after the fifth reuse cycle. Notably, this work provides new insight into the production of efficient, reusable, and enhanced photocatalysts using a rapid and cost-effective microwave-assisted synthesis process for pollutant remediation.
    MeSH terms: Adsorption; Catalysis; Kinetics; Photolysis*; Thermodynamics*; Water Pollutants, Chemical/chemistry
  16. Seng KBH, Tan PY, Lim CC, Loganathan R, Lim YA, Teng KT, et al.
    Nutr Res, 2024 Nov;131:14-26.
    PMID: 39357258 DOI: 10.1016/j.nutres.2024.07.003
    Vitamin A deficiency (VAD) remains a major health issue in developing nations, contributing to preventable childhood blindness. However, there is lack of recent data on xerophthalmia, especially among school-aged children in Malaysia. We hypothesized that xerophthalmia persists among rural schoolchildren in Malaysia and potentially associated with socio-demographic status and malnutrition. We conducted a cross-sectional study on 596 schoolchildren (8-12 years) from ten rural primary schools located in five states across Malaysia. Children meeting the criteria for xerophthalmia assessment included those diagnosed with vitamin A deficiency (VAD) (plasma retinol < 0.70 µmol/L) and marginal VAD (plasma retinol 0.70 to < 1.05 µmol/L). The overall prevalence of xerophthalmia was 48.8%, with the most common ocular sign being conjunctival xerosis (38.9%). The occurrence of xerophthalmia was negatively associated with retinol-binding protein 4 (RPB4) (P=0.003), alpha-carotene (P=0.04), hemoglobin (P=0.004), weight (P=0.02), body mass index (BMI) (P=0.04) and WAZ (weight-for-age z-score) (P=0.04) status. Based on multivariate logistic regression analysis, a higher risk of xerophthalmia was observed in boys (Adjusted odd ratio [AOR]: 1.7, 95% confidence interval [CI]: 1.2-2.5) and Orang Asli (OA, indigenous) schoolchildren (AOR: 2.0, 95% CI: 1.3-3.0), while schoolchildren with overweight/obesity status (AOR: 0.5, 95% CI: 0.3-0.8) were associated with a reduced risk of xerophthalmia. The present study unveils a high prevalence of xerophthalmia among vitamin A-deficient primary schoolchildren in rural areas of Malaysia, especially among the indigenous community. The identified socio-demographic and nutritional factors associated to xerophthalmia would facilitate the implementation of more targeted interventions in addressing these issues.
    MeSH terms: Child; Cross-Sectional Studies; Female; Humans; Malaysia/epidemiology; Male; Nutritional Status*; Schools; Socioeconomic Factors; Body Mass Index*; Prevalence; Malnutrition/epidemiology
  17. Chai AWY, Tan YH, Ooi S, Yee PS, Yee SM, Lightfoot H, et al.
    Cancer Res Commun, 2024 Nov 01;4(11):2919-2932.
    PMID: 39360810 DOI: 10.1158/2767-9764.CRC-24-0136
    Mechanistically guided drug repurposing has been made possible by systematically integrating pharmacologic and CRISPR-Cas9 screen data. Our study discovers the biomarker and cell death mechanisms underpinning sensitivity toward AZD5582, an antagonist of the inhibitor of apoptosis family protein. Our findings have important implications for improving future trial design for patients with OSCC using this emerging drug class.
    MeSH terms: Carcinoma, Squamous Cell/drug therapy; Carcinoma, Squamous Cell/genetics; Carcinoma, Squamous Cell/pathology; Humans; Mouth Neoplasms/drug therapy; Mouth Neoplasms/genetics; Mouth Neoplasms/metabolism; Mouth Neoplasms/pathology; Signal Transduction/drug effects; Cell Line, Tumor; Drug Repositioning/methods
  18. Alghamdi A, A Awadh Ali N, Alafnan A, Zainal Abidin SA, Alamri A, Hussein W, et al.
    Food Chem Toxicol, 2024 Nov;193:115028.
    PMID: 39368542 DOI: 10.1016/j.fct.2024.115028
    This study explores the phytochemical composition and biological activities of Verbascum yemenense, a plant known for its medicinal properties. The plant extract revealed a rich presence of bioactive compounds that exhibited significant antioxidant properties against free radicals. The enzyme inhibition potential was particularly notable against cholinesterases (AChE: 2.56 mg GALAE/g; BChE: 1.98 mg GALAE/g), and tyrosinase (87.94 mg KAE/g), α-glucosidase suggesting potential therapeutic applications in neurodegenerative diseases, skin disorders and diabetes. Molecular docking studies and Molecular Dynamics simulations, providing insights into the interaction mechanisms of the identified compounds with the target proteins. Molecular docking studies revealed high binding affinities of the phytoconstituents, with compounds like VY4 and phyllanthusol-A (VY15) showing substantial docking scores against AChE (-9.840 kcal/mol) and BChE (-9.853 kcal/mol), respectively. For instance, the RMSD values during the MD simulations for compound VY17 in the AML complex showed a stable conformation, fluctuating within a range of 0.75 Å to 1.75 Å, indicating a strong and consistent interaction with the enzyme. MESP studies highlighted VY17's distinctive electrostatic features, notably a pronounced electronegative region, which might contribute to its binding efficiency. These findings suggest that V. yemenense is a promising candidate for developing novel therapeutic agents.
    MeSH terms: Acetylcholinesterase/metabolism; Acetylcholinesterase/chemistry; Antioxidants/pharmacology; Antioxidants/chemistry; Butyrylcholinesterase/metabolism; Butyrylcholinesterase/chemistry; Cholinesterase Inhibitors/chemistry; Humans; Monophenol Monooxygenase/antagonists & inhibitors; Monophenol Monooxygenase/metabolism; Monophenol Monooxygenase/chemistry; Computational Biology; Molecular Dynamics Simulation*; Molecular Docking Simulation*; Phytochemicals/pharmacology; Phytochemicals/chemistry
  19. Zakaria ND, Salih IL, Hamzah HH, Sönmez T, Omar MH, Nor NM, et al.
    Analyst, 2024 Nov 04;149(22):5401-5410.
    PMID: 39373774 DOI: 10.1039/d4an00990h
    Sulfuric acid is commonly used to electrochemically activate gold electrodes in a variety of electrochemical applications. This work provides the first evaluations of the electrochemical behaviors and a 3D image of an activated screen-printed gold electrode (SPGE, purchased commercially) through electrochemical and imaging analyses. The activated SPGE surface appears rougher than the unactivated SPGE surface when viewed through microtopography images using scanning electron microscopy (SEM) and atomic force microscopy (AFM). Nevertheless, the roughened microscopy structure does not exhibit any substantial changes in roughness factor for the activated SPGE, as indicated by capacitive current analyses. The significant improvement in electrochemical responsiveness of the activated SPGE is mainly attributed to the presence of surface pores created in the microscopic structure as a result of gold oxide layer formation. The presence of surface pores on the activated surface has significantly improved its conductivity by 10-fold. As a result, electron transfer kinetics and mass transports of the activated SPGE are greatly improved. The results presented in this work indicate that the surface of the activated SPGE greatly increased its intrinsic surface pores, and conductivity of the electrode surface and uncovered the electrocatalytic active sites. This significantly improves the activated SPGE's performance in electrochemical applications such as oxygen reduction reaction (ORR). An activated SPGE considerably enhanced limiting current density as well as ∼172 mV versus Ag shifted onset potential to more positive potentials compared to unactivated SPGE.
  20. Memon MA, Osland E, Yunus RM, Hoque Z, Alam K, Khan S
    Surg Endosc, 2024 Nov;38(11):6254-6269.
    PMID: 39384655 DOI: 10.1007/s00464-024-11303-x
    BACKGROUND: To evaluate 5-year effect of laparoscopic vertical sleeve gastrectomy (LVSG) versus laparoscopic roux-en-Y gastric bypass (LRYGB) on gastroesophageal reflux disease (GERD) solely based on randomized controlled trials (RCTs).

    METHODS: A systematic review and meta-analysis of 5-year postoperative GERD data comparing LVSG and LRYGB in adults were undertaken. Electronic databases were searched from January 2015 to March 2024 for publications meeting inclusion criteria. The Hartung-Knapp-Sidik-Jonkman random effects model was applied to estimate pooled odds ratio where meta-analysis was possible. Bias and certainty of evidence were assessed using the Cochrane Risk of Bias Tool 2 and GRADE.

    RESULTS: Five RCTs were analysed (LVSG n = 554, LRYGB n = 539). LVSG was associated with increased adverse GERD outcomes compared to LRYGB at 5 years. The odds for revisional surgery to treat GERD in LVSG patients were 11 times higher compared to LRYGB (OR 11.47, 95% CI 1.83 to 71.69; p = 0.02; I2 = 0% High level of certainty). Similarly pharmacological management for increasing GERD was significantly more frequent in LVSG patients compared to LRYGB (OR 3.89, 95% CI 2.31 to 6.55; p ≤ 0.01; I2 = 0% Moderate level of certainty). Overall, LVSG was associated with significantly more interventions (both medical and surgical) for either worsening GERD and/or development of de novo GERD compared to LRYGB (OR 5.98, 95% CI 3.48 to 10.29; p ≤ 0.01; I2 = 0%) Moderate level of certainty).

    CONCLUSIONS: The development and worsening of GERD symptoms are frequently associated with LVSG compared to LRYGB at 5 years postoperatively requiring either initiation or increase of pharmacotherapy or failing that revisional bariatric surgery. Appropriate patient/surgical selection is crucial to reduce these postoperative risks of GERD.

    MeSH terms: Humans; Obesity, Morbid/surgery; Reoperation/statistics & numerical data; Randomized Controlled Trials as Topic; Treatment Outcome
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