Browse publications by year: 2025

  1. Raghvan HP, Ling CHS, Yui WS, Liang BY, Jamian E, Subbiah I
    J Yeungnam Med Sci, 2025;42:17.
    PMID: 39716342 DOI: 10.12701/jyms.2025.42.17
    Chronic myeloid leukemia (CML) typically progresses from a chronic phase to an accelerated phase, and eventually to a blast crisis, often involving the bone marrow and peripheral blood, if left untreated. Central nervous system (CNS) involvement is an uncommon manifestation of CML, particularly as an isolated CNS relapse. Here, we present a rare case of CML in lymphoid blast crisis with an isolated CNS relapse. A 46-year-old female with underlying CML in lymphoid blast crisis, previously treated with chemotherapy and tyrosine kinase inhibitors, presented with visual disturbances. Imaging and cerebrospinal fluid analysis confirmed leukemic infiltration of the CNS without evidence of a systemic disease. Isolated CNS involvement is an atypical complication of CML and presents significant therapeutic challenges owing to the blood-brain barrier, which limits the efficacy of systemic therapies. Subsequently, the patient was treated with intrathecal chemotherapy targeting the CNS. Despite aggressive treatment, CNS relapse remains a major concern due to the limited penetration of standard therapies into the CNS. This case underscores the importance of early recognition of CNS symptoms in CML patients, particularly in those with blast crisis, and highlights the need for tailored therapeutic strategies to manage this rare and challenging manifestation.
  2. Zohora FT, Pathmanathan R, Chowdhury EH
    ACS Appl Bio Mater, 2025 Jan 20;8(1):348-367.
    PMID: 39723844 DOI: 10.1021/acsabm.4c01319
    Naked siRNAs are sensitive to enzymatic degradation, phagocytic entrapment, quick renal excretion, membrane impermeability, endosomal escape, and off-target effects. Designing a safe and efficient nanocarrier for siRNA delivery to the target site without toxicity remains a significant hurdle in gene therapy. CA is a unique derivative of hydroxyapatite and a highly pH-sensitive nanocarrier with strong particle aggregation and a high polydispersity index. Strontium (Sr2+), a group two divalent metal in the periodic table, has been reported for substituting calcium (Ca2+) ions from the apatite lattice and limiting particle growth/aggregation. This study used strontium chloride hexahydrate (SrCl2·6H2O) salt to develop a Sr-substituted CA (Sr-CA) nanocarrier with ∼30 nm size, spherical shape, less aggregation, homodispersity, and a fair anionic charge. Sr-CA demonstrated a large surface area-to-volume ratio, an improved cargo loading efficiency, and enhanced cellular uptake in HEK-293 cells. Moreover, Sr-CA is a pH-responsive nanocarrier responsible for its long physiological stability, efficient endosomal escape, and optimal cargo delivery within cells. These NPs have differential effects on MAPK1, MAP2K4, PIK3Ca, CAMK4, and p53 gene expression in HEK-293 cells without showing any significant cytotoxicity in cell growth properties. Gene silencing by Sr-CA-mediated siRNA delivery against MAPK1, MAP2K4, PIK3Ca, and CAMK4 genes significantly decreased the level of target gene expression and cell survival, demonstrating successful intracellular siRNA delivery in HEK-293 cells. Additionally, biocompatibility testing confirmed the biological safety of the Sr-CA nanocarrier in mice. These findings suggest that Sr-CA nanocarriers are a promising siRNA delivery system, combining high efficiency with pH-sensitive release and excellent biocompatibility, making them a viable option for future therapeutic applications.
    MeSH terms: Animals; Cell Survival/drug effects; Drug Carriers/chemistry; Humans; Hydrogen-Ion Concentration; Materials Testing*; Particle Size*; Mice; Nanoparticles/chemistry; HEK293 Cells
  3. Jeffery N, Mock PY, Yang K, Tham CL, Israf DA, Li H, et al.
    Future Med Chem, 2025 Jan;17(2):237-257.
    PMID: 39727147 DOI: 10.1080/17568919.2024.2447226
    Methamphetamine (METH) is a highly addictive illicit psychostimulant with a significant annual fatality rate. Emerging studies highlight its role in neuroinflammation and a range of neurological disorders. This review examines the current landscape of potential drug targets for managing neuroinflammation in METH use disorders (MUDs), with a particular focus on the rationale behind targeting Toll-like receptor 4 (TLR4), the NLR family pyrin domain containing 3 (NLRP3) inflammasome, and other promising targets. Given the multifactorial neurological effects of METH, including cognitive impairment and neurodegeneration, addressing METH-induced neuroinflammation has shown considerable promise in partially mitigating the damaging effects on the central nervous system and improving behavioral outcomes. This article provides an overview of the existing understanding while charting a promising path forward for developing innovative MUD treatments, focusing on neuroinflammation as a therapeutic target. Targeting neuroinflammation in METH-induced neurological disorders shows significant promise in mitigating cognitive impairment and neurodegeneration, offering a potential therapeutic strategy for improving outcomes in MUD. While challenges remain in optimizing treatments, ongoing research into combination therapies, novel drug delivery systems, and neuroprotective agents suggests a positive outlook for more effective interventions.
    MeSH terms: Central Nervous System Stimulants/pharmacology; Animals; Humans; Inflammation/drug therapy; Inflammation/metabolism; Methamphetamine*; Neuroprotective Agents/pharmacology; Neuroprotective Agents/chemistry; Toll-Like Receptor 4/antagonists & inhibitors; Toll-Like Receptor 4/metabolism; Inflammasomes/metabolism; NLR Family, Pyrin Domain-Containing 3 Protein/antagonists & inhibitors; NLR Family, Pyrin Domain-Containing 3 Protein/metabolism
  4. Jeong JH, Saniasiaya J, McCaffer C
    Int J Pediatr Otorhinolaryngol, 2025 Feb;189:112234.
    PMID: 39826238 DOI: 10.1016/j.ijporl.2025.112234
    OBJECTIVE: Aspirated foreign bodies (FB) are potentially life-threatening conditions which can be challenging to diagnose in children. The previous similar study from our hospital, published in 2014, created a guideline suggesting when to proceed to laryngobronchoscopy (LB) in suspected FB aspiration with over 99 % sensitivity. The grading system included the presence or absence of acute history, positive examination, and/or radiological findings, including witnessed choking, dysphonia, stridor, wheezing, or reduced air entry on examination or abnormal radiological findings. The current study reviewed a further 10 years and 5 months of data to re-evaluate the ongoing diagnostic usefulness of the three positive findings in children with suspicion of aspirated FB.

    METHODS: We undertook a retrospective review of all LB performed at our institution for suspected acute FB aspiration from August 2013 to January 2023. We analysed patient characteristics, clinical examination, radiological findings, and outcomes.

    RESULTS: We found a total of 100 children under the age of 16 years who underwent LB for suspected FB aspiration. The mean age of our study population was 2.6 years. The population demographics were similar to the results from the previous study from our institution, with Māori and Pasifika children having the highest probability of finding a FB on LB. Male children presenting for suspected FB aspiration are 1.39 times more likely than female children to have a foreign body at LB (p = 0.04). Overall, having two or more positive findings had a sensitivity of 100 %. History or examination alone are more sensitive (both 95.6 %) but less specific (12.5 % and 31.3 %, respectively). Abnormal radiology alone was more specific (75.0) but less sensitive (77.9 %). The most common site of FB was the right bronchial tree (45.6 %), followed by the left (39.7 %). 95 % of FB were organic, and 54 % were nuts. The negative bronchoscopy rate was 32 %.

    CONCLUSION: Two or more positive indicators in history, examination and radiological findings continue to be highly sensitive predictors for the presence of an aspirated foreign body in our paediatric population. We recommend a future large-scale multicentre study to confirm how applicable these findings would be to a broader cohort of patients.

    MeSH terms: Adolescent; Airway Obstruction/diagnosis; Airway Obstruction/etiology; Bronchoscopy*; Child; Child, Preschool; Female; Humans; Infant; Laryngoscopy*; Male; New Zealand; Retrospective Studies; Respiratory Aspiration/diagnosis; Tertiary Care Centers*
  5. Chan JC, Lee CT, Say YH, Lin YF, Tsai MC
    J Psychiatr Res, 2025 Feb;182:277-283.
    PMID: 39826378 DOI: 10.1016/j.jpsychires.2025.01.017
    BACKGROUND: Adverse childhood events (ACEs) have been associated with an increased risk of psychiatric disorders in young adulthood. To identify at-risk individuals and potential strategies to combat the negative impacts of ACE, this study investigated the mediating role of exercise in the relationship between psychological distress and ACEs. Further, we examined the moderating effect of the BDNF polymorphism in the mediation relationship.

    METHODS: Participants (N = 750, Mage = 20.1 years) completed questionnaires assessing ACEs divided into adverse environment (AE) and childhood maltreatment (CM), exercise, and psychological distress. Salivary genomic DNA was used for genotyping. The significance of the moderated mediation model was assessed using bootstrapping.

    RESULTS: There was a significant association between ACEs and psychological distress mediated by exercise. After addition of BDNF polymorphism, we found that the effect of ACEs on psychological distress through exercise was moderated by the BDNF polymorphism (index of moderated mediation = -0.19, [-0.48, -0.04], p-value ≤0.05). Further dividing ACE into AE and CM, the moderated mediation relationship remains significant only with AE (index of moderated mediation = -0.41, [-0.99, -0.10], p-value ≤0.05).

    CONCLUSIONS: The interaction between BDNF polymorphism and exercise may be a suitable target for interventions in ACEs-experienced individuals for the prevention or reduction of psychological distress.

    MeSH terms: Adult Survivors of Child Adverse Events/statistics & numerical data; Adolescent; Adult; Child Abuse/psychology; Child Abuse/statistics & numerical data; Female; Humans; Male; Stress, Psychological; Polymorphism, Single Nucleotide; Adult Survivors of Child Abuse/statistics & numerical data; Young Adult; Gene-Environment Interaction
  6. Hassan RA, Abu Hanifah S, Heng LY
    Talanta, 2025 May 15;287:127592.
    PMID: 39826470 DOI: 10.1016/j.talanta.2025.127592
    The scientific literature on molecularly imprinted polymers (MIPs) has grown significantly in the past decades, reflecting an increasing interest in their potential applications. MIPs are valued for their ability to selectively detect a broad range of analytes and mimic biological recognition in different environmental conditions. This review utilises data (Scopus data from 2010 to 2024) from a bibliometric visualisation with VOSviewer (version 1.6.2) to identify trends and research hotspots in developing MIP-based sensors. The findings from this review indicated notable advancements in molecular imprinting technology (MIT) and the challenges MIP technology faces. It also discusses how various optimisation preparation techniques can be used to overcome the inherent limitations of MIP synthesis. The review also presents a case investigation and suggests classifying MIPs as chemosensors (chemical sensors) rather than biosensors to resolve the confusion and classification difficulties encountered in the existing literature on MIP sensors. It also addresses critical issues regarding the paradoxical lack of MIP-based sensors in the commercial market despite a marked increase in scientific output. The review outlines future research directions to enhance MIP sensor technology further. It emphasises the need for more collaboration between academia and industry to bridge existing gaps and accelerate commercialisation.
  7. Zulhafiz NA, Teoh TC, Chin AV, Chang SW
    Comput Methods Programs Biomed, 2025 Apr;261:108604.
    PMID: 39826482 DOI: 10.1016/j.cmpb.2025.108604
    BACKGROUND: Alzheimer's disease (AD), the most prevalent form of dementia, remains enigmatic in its origins despite the widely accepted "amyloid hypothesis," which implicates amyloid-beta peptide aggregates in its pathogenesis and progression. Despite advancements in technology and healthcare, the incidence of AD continues to rise. The traditional drug development process remains time-consuming, often taking years to bring an AD treatment to market. Drug repurposing has emerged as a promising strategy for developing cost-effective and efficient therapeutic options by identifying new uses for existing approved drugs, thus accelerating drug development.

    OBJECTIVES: This study aimed to examine two key drug repurposing methodologies in general diseases and specifically in AD, which are artificial intelligent (AI) approach and molecular docking approach. In addition, the hybrid approach that integrates AI with molecular docking techniques will be explored too.

    METHODOLOGY: This study systematically compiled a comprehensive collection of relevant academic articles, scientific papers, and research studies which were published up until November 2024 (as of the writing of this review paper). The final selection of papers was filtered to include studies related to Alzheimer's disease and general diseases, and then categorized into three groups: AI articles, molecular docking articles, and hybrid articles.

    RESULTS: As a result, 331 papers were identified that employed AI for drug repurposing in general diseases, and 58 papers focused specifically in AD. For molecular docking in drug repurposing, 588 papers addressed general diseases, while 46 papers were dedicated to AD. The hybrid approach combining AI and molecular docking in drug repurposing has 52 papers for general diseases and 9 for AD. A comparative review was done across the methods, results, strengths, and limitations in those studies. Challenges of drug repurposing in AD are explored and future prospects are proposed.

    DISCUSSION AND CONCLUSION: Drug repurposing emerges as a compelling and effective strategy within AD research. Both AI and molecular docking methods exhibit significant potential in this domain. AI algorithms yield more precise predictions, thus facilitating the exploration of new therapeutic avenues for existing drugs. Similarly, molecular docking techniques revolutionize drug-target interaction modelling, employing refined algorithms to screen extensive drug databases against specific target proteins. This review offers valuable insights for guiding the utilization of AI, molecular docking, or their hybrid in AD drug repurposing endeavors. The hope is to speed up the timeline of drug discovery which could improve the therapeutic approach to AD.

    MeSH terms: Artificial Intelligence*; Humans; Drug Repositioning*; Molecular Docking Simulation*
  8. Yin J, Waman VP, Sen N, Firdaus-Raih M, Lam SD, Orengo C
    Structure, 2025 Jan 15.
    PMID: 39826548 DOI: 10.1016/j.str.2024.12.016
    ATP-pyrophosphatases (ATP-PPases) are the most primordial lineage of the large and diverse HUP (high-motif proteins, universal stress proteins, ATP-pyrophosphatase) superfamily. There are four different ATP-PPase substrate-specificity groups (SSGs), and members of each group show considerable sequence variation across the domains of life despite sharing the same catalytic function. Owing to the expansion in the number of ATP-PPase domain structures from advances in protein structure prediction by AlphaFold2 (AF2), we have characterized the two most populated ATP-PPase SSGs, the nicotinamide adenine dinucleotide synthases (NADSs) and guanosine monophosphate synthases (GMPSs). Local structural and sequence comparisons of NADS and GMPS identified taxonomic-group-specific functional motifs. As GMPS and NADS are potential drug targets of pathogenic microorganisms including Mycobacterium tuberculosis, bacterial GMPS and NADS specific functional motifs reported in this study, may contribute to antibacterial-drug development.
  9. Fong KY, Yuen SKK, Somani BK, Malkhasyan V, Tanidir Y, Persaud S, et al.
    Urology, 2025 Jan 16.
    PMID: 39826806 DOI: 10.1016/j.urology.2025.01.029
    OBJECTIVE: To study the long-term outcomes of the flexible and navigable suction ureteral access sheath (FANS) in flexible ureteroscopy (FURS). FANS has well-established efficiency and 30-day perioperative safety; however, its influence on pelvicalyceal and ureteric anatomy remains to be investigated.

    METHODS: This was a prospective study of patients with normal renal anatomy who underwent FURS with FANS for renal stones from April 2023 to August 2024 in 16 centers worldwide. Thirty-day postoperative stone-free rate and delayed complications after 3 months were analyzed with a non-contrast CT scan and contrasted CT urogram respectively. The primary aim was to report if FANS could potentially cause pelviureteric junction, pelvicalyceal or ureteric complications.

    RESULTS: Three hundred-ten patients were analyzed. Median age was 51; median stone volume was 1584 mm3. Disposable scopes were used in 58.1%. Predominant laser energies were Thulium fiber laser (56.1%) followed by Thulium-YAG laser (20.6%). Median laser time was 14 minutes, median ureteroscopy time was 30 minutes, and median total operation time was 45 minutes. Ureteric injury occurred in 11 patients (3.5%), of which 10 were Traxer-Thomas Grade 1. On 30-day NCCT, 63.5% of patients had zero residual fragments; overall stone-free status was seen in 95.1%. Thirty-day reintervention rate was 4.2% (13 cases). Three-month CT urogram showed ureteric stenosis in only 1 patient (0.3%) who was managed by dilatation and stenting. No other anatomical anomalies were recorded.

    CONCLUSION: The extremely low rates of 3-month adverse outcomes, high immediate SFR, and low 30-day reintervention rates further strengthen the evidence for safety and effectiveness of FANS.

  10. St Peter SD, Noel-MacDonnell JR, Hall NJ, Eaton S, Suominen JS, Wester T, et al.
    Lancet, 2025 Jan 18;405(10474):233-240.
    PMID: 39826968 DOI: 10.1016/S0140-6736(24)02420-6
    BACKGROUND: Support for the treatment of uncomplicated appendicitis with non-operative management rather than surgery has been increasing in the literature. We aimed to investigate whether treatment of uncomplicated appendicitis with antibiotics in children is inferior to appendicectomy by comparing failure rates for the two treatments.

    METHODS: In this pragmatic, multicentre, parallel-group, unmasked, randomised, non-inferiority trial, children aged 5-16 years with suspected non-perforated appendicitis (based on clinical diagnosis with or without radiological diagnosis) were recruited from 11 children's hospitals in Canada, the USA, Finland, Sweden, and Singapore. Patients were randomly assigned (1:1) to the antibiotic or the appendicectomy group with an online stratified randomisation tool, with stratification by sex, institution, and duration of symptoms (≥48 h vs <48 h). The primary outcome was treatment failure within 1 year of random assignment. In the antibiotic group, failure was defined as removal of the appendix, and in the appendicectomy group, failure was defined as a normal appendix based on pathology. In both groups, failure was also defined as additional procedures related to appendicitis requiring general anaesthesia. Interim analysis was done to determine whether inferiority was to be declared at the halfway point. We used a non-inferiority design with a margin of 20%. All outcomes were assessed in participants with 12-month follow-up data. The trial was registered at ClinicalTrials.gov (NCT02687464).

    FINDINGS: Between Jan 20, 2016, and Dec 3, 2021, 936 patients were enrolled and randomly assigned to appendicectomy (n=459) or antibiotics (n=477). At 12-month follow-up, primary outcome data were available for 846 (90%) patients. Treatment failure occurred in 153 (34%) of 452 patients in the antibiotic group, compared with 28 (7%) of 394 in the appendicectomy group (difference 26·7%, 90% CI 22·4-30·9). All but one patient meeting the definition for treatment failure with appendicectomy were those with negative appendicectomies. Of those who underwent appendicectomy in the antibiotic group, 13 (8%) had normal pathology. There were no deaths or serious adverse events in either group. The relative risk of having a mild-to-moderate adverse event in the antibiotic group compared with the appendicectomy group was 4·3 (95% CI 2·1-8·7; p<0·0001).

    INTERPRETATION: Based on cumulative failure rates and a 20% non-inferiority margin, antibiotic management of non-perforated appendicitis was inferior to appendicectomy.

    FUNDING: None.

    MeSH terms: Acute Disease; Adolescent; Appendectomy*; Canada; Child; Child, Preschool; Female; Humans; Male; Singapore; Treatment Failure
  11. Amiri MS, Ramli R, Van M
    ISA Trans, 2025 Mar;158:640-653.
    PMID: 39827031 DOI: 10.1016/j.isatra.2025.01.003
    In recent years, exoskeleton robots have attracted great interest from researchers in the area of robotics due to their ability to assist human functionality improvement. A wearable lower limb exoskeleton is aimed at supporting the limb functionality rehabilitation process and to assist physical therapists. Development of a stable and robust control system for multi-joint rehabilitation robots is a challenging task due to their non-linear dynamic systems. This paper presents the development of a Swarm-Initialized Adaptive (SIA) based controller, which is a combination of a swarm-based intelligence, named Swarm Beetle Antenna Searching (SBAS), and an adaptive Lyapunov-based controller. The SBAS initializes the parameters of SIA to efficiently improve the performance of the control system and then these controller parameters are updated by an adaptive controller. The control system is validated in a lower limb exoskeleton prototype with four degrees of freedom, using a healthy human subject for sit-to-stand and walking motions. The experimental results show the applicability of the proposed method and demonstrate that our approach obtained efficient control performance in terms of steady-state error and robustness and can be used for a lower limb exoskeleton to improve human mobility.
    MeSH terms: Exoskeleton Device*; Algorithms*; Animals; Artificial Intelligence; Beetles/physiology; Biomechanical Phenomena; Equipment Design; Humans
  12. Tasiou A, Tzerefos C, Karagianni M, Tsianaka E, Jarratt M, Gazioğlu N, et al.
    World Neurosurg, 2025 Feb 08;195:123692.
    PMID: 39827952 DOI: 10.1016/j.wneu.2025.123692
    OBJECTIVE: Neurosurgical care is difficult to access in many scenarios. Aeromedical evacuation of acutely unwell neurosurgical patients from remote, isolated, or poorly equipped locations can be considered. This article aims to provide a framework of logistical factors that deserve special consideration in the preparation of these patients for transfer.

    METHODS: We searched all relevant medical literature, military reports, and travel industry documents on transfer of neurosurgical patients. This review was combined with a senior author's (M.J.) extensive relevant experience, to present important factors for neurosurgeons to consider during planning of aeromedical evacuation, highlighting potential preventable causes of deterioration en route.

    RESULTS: Several criteria must be met for a transfer to be considered. The safe transfer of patients with craniospinal pathology requires efficient collaboration between the referring teams, the receiving units/departments, and the medical transfer service. Clear communication, qualified personnel, and appropriate transportation equipment must be available for the transfer. One must consider unique stressors during the air transfer, including the risk of hypoxia on certain types of flights. Vibration, loud noise, acceleration, and changes in barometric pressure en route may negatively affect the patient during transfer. Patient stabilization before transfer is a priority. Medical conditions that can potentially worsen in-flight should be corrected before transfer. The use of a checklist before departure is highly recommended and is included herein. The timing of transfer concerning the postoperative patient deserves special consideration.

    CONCLUSIONS: Although there is little published information, this review provides useful criteria and parameters needed for safe aeromedical evacuation of neurosurgical patients.

  13. Lim TW, Abuzaid MM, Muhammad Zulhisham MFAH, Muhamad Hanafiah NN, Mohd Zahari HL, Mahmud M, et al.
    J Dent, 2025 Mar;154:105585.
    PMID: 39828021 DOI: 10.1016/j.jdent.2025.105585
    OBJECTIVE: The present study aimed to investigate the impact of utilizing an innovative and comprehensive patient-centered digital clinical decision aid designed to facilitate shared decision-making in missing tooth replacement between dental students and their patients.

    METHODS: A qualitative study using interview approach was conducted in Hong Kong SAR and Malaysia. Thirty dental undergraduate students, each with one patient from the Faculty of Dentistry at The University of Hong Kong (n = 15) and Universiti Teknologi MARA (n = 15), were introduced to a digitally designed decision aid in missing tooth replacement prior to their treatment appointments. Semi-structured interviews were conducted with each student and patient, adhering to the interview protocol. Each interview was audio-recorded, transcribed, and subsequently coded to investigate the perceptions and potential advantages of this decision aid.

    RESULTS: Thematic analysis identified three key themes from the dental students' perspective: communication, utilization, and satisfaction. From patients' perceptions, four central themes emerged: communication, treatment information, uncertainty, and utilization. Detailed examination of the data highlighted an enhancement in patients' confidence and trust in their dental care providers, as well as a marked increase in both student and patient satisfaction levels upon implementing this novel approach. The average satisfaction rates for students were 83 % for Hong Kong SAR and 82 % for Malaysia.

    CONCLUSION: This patient-centered clinical decision aid helped to enhance communication between dental students and patients in both regions, ultimately leading to heightened patient satisfaction levels. Nonetheless, to address the present study's limitations, future studies should consider diversifying participant backgrounds, including patients without prior treatment discussions with students.

    CLINICAL SIGNIFICANCE: Clinical decision aids are valuable tools in clinical teaching due to their enhancement of communication between clinicians and patients. They promote shared decision-making, leading to more personalized and evidence-driven treatment plans, ultimately improving patient care.

    MeSH terms: Adult; Communication; Decision Support Techniques; Dentist-Patient Relations; Female; Hong Kong; Humans; Malaysia; Male; Middle Aged; Patient Participation; Patient Satisfaction; Qualitative Research*; Young Adult
  14. Lim CC, Lim TS
    Int J Biol Macromol, 2025 Feb;290:140037.
    PMID: 39828167 DOI: 10.1016/j.ijbiomac.2025.140037
    Lymphatic filariasis is caused by infections of thread-like filarial worms, namely Wuchereria bancrofti, Brugia Malayi and Brugia timori. However, in-depth analysis of the antibody repertoire against Lymphatic filariasis is lacking. Using high-throughput sequencing of antibody repertoires, immunome analysis of IgG (LG) and IgM (LM) repertoires were studied. Despite significant differences between LG and LM in V(D)J gene usage, IGHV4-34, IGHV6-1, IGHD3-10 and IGHJ4 were preferred in both repertoires. The CDR3 in the LG repertoire showed a longer length than LM. Higher SHM level were observed in LG sequences and presence of oligoclonal sequences indicates the extent of clonal expansion. The prevalence of rare clonotypes in LM repertoire depicts the high clonal diversity when compared to LG repertoire. Monoclonal antibodies against closely related parasitic infections were present within the LG repertoire suggesting that immune repertoires may not be as exclusive and biased against the target infection as initially thought. The characterization of the immune repertoire can provide critical insight into the antibody response patterns in disease state, antibody generation process during infections and future antibody designs.
    MeSH terms: Animals; Antibodies, Helminth/immunology; Antibody Diversity/immunology; Humans; Immunoglobulin G/genetics; Immunoglobulin G/immunology; Immunoglobulin M/immunology; High-Throughput Nucleotide Sequencing*
  15. Tahir D, Ardiansyah A, Heryanto H, Noor EEM, Mohamed MA
    Int J Biol Macromol, 2025 Apr;298:140010.
    PMID: 39828168 DOI: 10.1016/j.ijbiomac.2025.140010
    This article provides a comprehensive review of chitosan-based hydrogels for transdermal drug delivery. It covers various aspects including the chemical structure of chitosan and its derivatives, crosslinking agents, hydrogel morphology, and drug loading and release behaviors. The review draws on 16 studies sourced from Scopus, focusing on how the composition and structure of hydrogels influence drug release. The chemical structure of chitosan and its derivatives, such as chitosan hydrochloride and quaternary ammonium chitosan, plays a crucial role in determining the mechanical properties and stability of hydrogels. Crosslinking agents, both chemical and physical, affect the hydrogel's strength and transparency as well as its drug release profile. Hydrogel morphology, including its porosity, significantly impacts drug loading capacity and release behavior. Hydrogel-based microneedles show potential in overcoming the limitations of traditional drug administration methods by enhancing skin penetration and drug loading efficiency. Additionally, factors such as swelling ratio, drug loading efficiency, and release behavior are influenced by crosslinking methods, material composition, and environmental conditions. This article concludes that the design and modification of chitosan-based hydrogels can optimize transdermal drug delivery by improving drug release profiles and stability.
    MeSH terms: Administration, Cutaneous*; Animals; Drug Carriers/chemistry; Humans; Skin/drug effects; Skin/metabolism; Skin Absorption; Drug Delivery Systems*; Drug Liberation*
  16. Ng YS, Zheng JC
    ACS Omega, 2025 Jan 14;10(1):904-911.
    PMID: 39829495 DOI: 10.1021/acsomega.4c08031
    Using the free energy of hydrogen adsorption (ΔG H ) as the indicator, five borophene phases are previously shown to possess high catalytic activity for the hydrogen evolution reaction (HER). On these borophene phases, we investigate the role of the coordination number (CN = 4, 5, 6) of the adsorption sites and the puckering of the adsorption site. CN is discovered to have a profound effect on the ΔG H distribution, charge, and puckering height (h) of adsorption sites. Employing bonding indicators, we investigated the puckering mechanism. An increase in h directly increases the bond order and bond strength of the adsorption bond, but the associated energy cost of bond stretching hinders further puckering. The occupation and localization of σ orbitals determine the strength of local σ bonds-the key factors determining h. External biaxial strain is revealed to monotonically modulate the 2s character in local σ bonds, thus affecting bond stretchability, altering h and ΔG H subsequently. These insights are valuable for borophene-based HER applications and provide an analytical framework for the adsorption on 2D materials.
  17. Chung HH, Soh AAL, Lau MML, Gan HM, Sim SF, Lim LWK
    Data Brief, 2025 Feb;58:111248.
    PMID: 39830615 DOI: 10.1016/j.dib.2024.111248
    The engkabang jantong (Rubroshorea macrophylla) is one of the most indispensable tree species for reforestation due to its high survival rate and rapid growth rate. Due to relatively low genetic interest of this tree species, its genomic landscape has since faced scarcity, impeding our further elucidation on genes that are involved in expressing its aforementioned superior properties. In this study, we performed genome survey and microsatellite analysis of engkabang jantong. Based on the results, the estimated genome size of this species is 312,071,515 bp with 18.43 % repeated sequences and 1.16 % heterozygosity. BUSCO analysis unearthed that 83.5 % of the contigs are single-copy genes whereas 12.7 % of them are duplicated. Only 2.8 % and 1 % of them are fragmented and missing respectively. The short-read sequencing results obtained from the Illumina platform in this study will be essential to complement the Nanopore long-read sequencing results in hybrid genome assembly endeavors in the near future.
  18. Lee MF, Long CM, Poh CL
    Vaccine X, 2025 Jan;22:100604.
    PMID: 39830640 DOI: 10.1016/j.jvacx.2024.100604
    Dengue fever is caused by the mosquito-borne dengue virus (DENV), which is endemic in more than 100 countries. Annually, there are approximately 390 million dengue cases, with a small subset manifesting into severe illnesses, such as dengue haemorrhagic fever or dengue shock syndrome. Current treatment options for dengue infections remain supportive management due to the lack of an effective vaccine and clinically approved antiviral. Although the CYD-TDV (Dengvaxia®) vaccine with an overall vaccine efficacy of 60 % has been licensed for clinical use since 2015, it poses an elevated risk of severe dengue infections especially in dengue-naïve children below 9 years of age. The newly approved Qdenga vaccine was able to achieve an overall vaccine efficacy of 80 % after 12 months, but it was not able to provide a protective effect against DENV-3 in dengue naïve individuals. The Butantan-DV vaccine candidate is still undergoing phase 3 clinical trials for safety and efficacy evaluations in humans. Apart from live-attenuated vaccines, various other vaccine types are also currently being studied in preclinical and clinical studies. This review discusses the current status of dengue vaccine development.
  19. Syazwan WM, Then AY, Chong VC, Rizman-Idid M
    PeerJ, 2025;13:e18483.
    PMID: 39830958 DOI: 10.7717/peerj.18483
    Population blooms of scyphozoan jellyfish in tropical shallow water regions can fuel localized fisheries but also negatively impact human welfare. However, there is a lack of baseline ecological data regarding the scyphozoans in the region, which could be used to manage a fast-growing fishery and mitigate potential impacts. Thus, this study aims to investigate the temporal factors driving the distribution of scyphozoan community along the environmental gradients under different monsoon seasons, rainfall periods, moon phases, and diel-tidal conditions in the Klang Strait located in the central region along the west coast of Peninsular Malaysia, where bloom events are increasing. Scyphozoan samples were collected using commercial bag nets during a 19-month survey. Temporal variations in species abundance and composition were evident and related to the local environmental parameters (salinity, dissolved oxygen, temperature, turbidity, and pH) that varied with the regional monsoon events, although these effects appeared to be species-specific. Phyllorhiza punctata, Acromitus flagellatus, Lychnorhiza malayensis, and Rhopilema esculentum were more abundant during the wetter northeast monsoon (NEM) while the abundance of Chrysaora chinensis and Lobonemoides robustus increased during the drier southwest monsoon (SWM). During the wet period of NEM, scyphozoan abundance was generally higher during the daytime than night-time. The regional monsoon regime and local hydrological events account for jellyfish abundance in the nearshore area with concurrent threats to coastal tourism and power plants, as well as benefits to fisheries especially during the NEM.
    MeSH terms: Animals; Malaysia; Seasons*; Tropical Climate*; Scyphozoa*; Biodiversity
  20. Xu YX, Niu XX, Jia WC, Wen J, Cheng XL, Han Y, et al.
    World J Psychiatry, 2025 Jan 19;15(1):101750.
    PMID: 39831008 DOI: 10.5498/wjp.v15.i1.101750
    BACKGROUND: The burden of mental disorders (MD) in the Western Pacific Region (WPR) remains a critical public health concern, with substantial variations across demographics and countries.

    AIM: To analyze the burden of MD in the WPR from 1990 to 2021, along with associated risk factors, to reveal changing trends and emerging challenges.

    METHODS: We used data from the Global Burden of Disease 2021, analyzing prevalence, incidence, and disability-adjusted life years (DALYs) of MD from 1990 to 2021. Statistical methods included age-standardisation and uncertainty analysis to address variations in population structure and data completeness.

    RESULTS: Between 1990 and 2021, the prevalence of MD rose from 174.40 million cases [95% uncertainty interval (UI): 160.17-189.84] to 234.90 million cases (95%UI: 219.04-252.50), with corresponding DALYs increasing from 22.8 million (95%UI: 17.22-28.79) to 32.07 million (95%UI: 24.50-40.68). During this period, the burden of MD shifted towards older age groups. Depressive and anxiety disorders were predominant, with females showing higher DALYs for depressive and anxiety disorders, and males more affected by conduct disorders, attention-deficit hyperactivity disorder, and autism spectrum disorders. Australia, New Zealand, and Malaysia reported the highest burdens, whereas Vietnam, China, and Brunei Darussalam reported the lowest. Additionally, childhood sexual abuse and bullying, and intimate partner violence emerged as significant risk factors.

    CONCLUSION: This study highlights the significant burden of MD in the WPR, with variations by age, gender, and nation. The coronavirus disease 2019 pandemic has exacerbated the situation, emphasizing the need for a coordinated response.

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