Browse publications by year: 2025

  1. Shah DP, Thaweethai T, Karlson EW, Bonilla H, Horne BD, Mullington JM, et al.
    JAMA Netw Open, 2025 Jan 02;8(1):e2455430.
    PMID: 39841477 DOI: 10.1001/jamanetworkopen.2024.55430
    IMPORTANCE: A substantial number of individuals worldwide experience long COVID, or post-COVID condition. Other postviral and autoimmune conditions have a female predominance, but whether the same is true for long COVID, especially within different subgroups, is uncertain.

    OBJECTIVE: To evaluate sex differences in the risk of developing long COVID among adults with SARS-CoV-2 infection.

    DESIGN, SETTING, AND PARTICIPANTS: This cohort study used data from the National Institutes of Health (NIH) Researching COVID to Enhance Recovery (RECOVER)-Adult cohort, which consists of individuals enrolled in and prospectively followed up at 83 sites in 33 US states plus Washington, DC, and Puerto Rico. Data were examined from all participants enrolled between October 29, 2021, and July 5, 2024, who had a qualifying study visit 6 months or more after their initial SARS-CoV-2 infection.

    EXPOSURE: Self-reported sex (male, female) assigned at birth.

    MAIN OUTCOMES AND MEASURES: Development of long COVID, measured using a self-reported symptom-based questionnaire and scoring guideline at the first study visit that occurred at least 6 months after infection. Propensity score matching was used to estimate risk ratios (RRs) and risk differences (95% CIs). The full model included demographic and clinical characteristics and social determinants of health, and the reduced model included only age, race, and ethnicity.

    RESULTS: Among 12 276 participants who had experienced SARS-CoV-2 infection (8969 [73%] female; mean [SD] age at infection, 46 [15] years), female sex was associated with higher risk of long COVID in the primary full (RR, 1.31; 95% CI, 1.06-1.62) and reduced (RR, 1.44; 95% CI, 1.17-1.77) models. This finding was observed across all age groups except 18 to 39 years (RR, 1.04; 95% CI, 0.72-1.49). Female sex was associated with significantly higher overall long COVID risk when the analysis was restricted to nonpregnant participants (RR, 1.50; 95%: CI, 1.27-1.77). Among participants aged 40 to 54 years, the risk ratio was 1.42 (95% CI, 0.99-2.03) in menopausal female participants and 1.45 (95% CI, 1.15-1.83) in nonmenopausal female participants compared with male participants.

    CONCLUSIONS AND RELEVANCE: In this prospective cohort study of the NIH RECOVER-Adult cohort, female sex was associated with an increased risk of long COVID compared with male sex, and this association was age, pregnancy, and menopausal status dependent. These findings highlight the need to identify biological mechanisms contributing to sex specificity to facilitate risk stratification, targeted drug development, and improved management of long COVID.

    MeSH terms: Adult; Aged; Female; Humans; Male; Middle Aged; Prospective Studies; Risk Factors; Sex Factors; United States/epidemiology; Cohort Studies
  2. Swami V, Stieger S, Voracek M, Aavik T, Abdollahpour Ranjbar H, Adebayo SO, et al.
    PLoS One, 2025;20(1):e0313107.
    PMID: 39841629 DOI: 10.1371/journal.pone.0313107
    The Satisfaction With Life Scale (SWLS) is a widely used self-report measure of subjective well-being, but studies of its measurement invariance across a large number of nations remain limited. Here, we utilised the Body Image in Nature (BINS) dataset-with data collected between 2020 and 2022 -to assess measurement invariance of the SWLS across 65 nations, 40 languages, gender identities, and age groups (N = 56,968). All participants completed the SWLS under largely uniform conditions. Multi-group confirmatory factor analysis indicated that configural and metric invariance was upheld across all nations, languages, gender identities, and age groups, suggesting that the unidimensional SWLS model has universal applicability. Full scalar invariance was achieved across gender identities and age groups. Based on alignment optimisation methods, partial scalar invariance was achieved across all but three national groups and across all languages represented in the BINS. There were large differences in latent SWLS means across nations and languages, but negligible-to-small differences across gender identities and age groups. Across nations, greater life satisfaction was significantly associated with greater financial security and being in a committed relationship or married. The results of this study suggest that the SWLS largely assesses a common unidimensional construct of life satisfaction irrespective of respondent characteristics (i.e., national group, gender identities, and age group) or survey presentation (i.e., survey language). This has important implications for the assessment of life satisfaction across nations and provides information that will be useful for practitioners aiming to promote subjective well-being internationally.
    MeSH terms: Adolescent; Adult; Age Factors; Aged; Factor Analysis, Statistical; Female; Gender Identity*; Humans; Language; Male; Middle Aged; Personal Satisfaction*; Psychometrics/methods; Quality of Life; Surveys and Questionnaires; Young Adult; Self Report
  3. Elnaem MH, Bukhori NAS, Tengku Mohd Kamil TK, Rahayu S, Ramatillah DL, Elrggal ME
    Psychol Health Med, 2025 Mar;30(3):555-571.
    PMID: 39841970 DOI: 10.1080/13548506.2025.2450545
    Patients with type 2 diabetes mellitus (T2DM) are susceptible to mental health issues, impacting medication adherence and diabetes control. This study aimed to evaluate factors associated with depression and anxiety among T2DM patients in Indonesia and Malaysia. A cross-sectional study was conducted in Indonesia and Malaysia from October 2022 to April 2023 among T2DM patients. The study utilised an instrument with patient and disease data and three validated tools to assess depression, anxiety, and medication adherence. Statistical analysis, including binary logistic regression, was performed using SPSS® version 28 software. A study of 606 T2DM patients revealed that 56.5% were at risk of depression, while 41.6% were at risk of anxiety. Older patients with T2DM had lower rates of depression (AOR = 0.41, 0.25-0.68) and anxiety than younger patients. Normal-weight patients were less likely to experience depression and anxiety (AOR = 0.44, 0.27-0.72) than overweight patients. Patients without diabetic foot ulcers had a lower risk of depression (AOR = 0.34, 0.21-0.55) and anxiety than those with foot ulcers. Patients with a shorter duration of diabetes had a higher risk of depression (AOR = 3.27, 1.70-6.30) and anxiety than those with a longer duration. Patients on insulin-based regimens had higher rates of depression and anxiety (AOR = 2.28, 1.20-4.30) than those on metformin-based regimens. Nonadherent patients were more likely to experience depression and anxiety (AOR = 4.30, 2.22-8.32) than patients who adhered to their medication. The prevalence of depression and anxiety is concerning and influenced by factors such as age, diabetes duration, the presence of diabetic foot ulcers, and the prescribed medication regimen. Further efforts are necessary to enhance the mental health of T2DM patients and improve management outcomes.
    MeSH terms: Adult; Age Factors; Aged; Cross-Sectional Studies; Female; Humans; Hypoglycemic Agents/therapeutic use; Indonesia/epidemiology; Malaysia/epidemiology; Male; Middle Aged; Time Factors; Diabetic Foot/epidemiology; Diabetic Foot/psychology
  4. Tukimin SN, Karman SB, Wan Kamarul Zaman WS, Mohd Yunos NB, Syed Nor SN, Ahmad MY
    PMID: 39842129 DOI: 10.1016/j.saa.2024.125503
    Light-matter interaction has been profoundly studied for sample material classification. However, the optical classification of the sample through the polarized light-matter interaction remains underexplored. It is limited to the measurement of intensity instead of the angle of polarized light (AOP) for its degree of polarization. Measurement of the degree of polarization within a material or a medium becomes easier with a simple, low-cost and direct measurement without the need of any probing or labelling agent. Thus, this investigation was conducted mainly to determine the angle of polarized light (AOP) property of the crosslinked polymer using our proposed polarization measurement technique as an alternative approach of the material classification. The angle of polarized light (AOP) of each polymer was determined in combination property of polarization by absorption, transmission, and scattering. Our proposed scattered angle (ס=90°, 100°, 110°, and 120°) successfully measured the AOP of each polymer that can be classified into two groups. Group 1 represents the AOP value ( [Formula: see text] ) for a test sample of t1 = 3.1 %, 3.2, and 3.3 % with comparison to the normal sample (n = 3.0 %) and Group 2 represents the AOP value ( [Formula: see text] ) for the test sample oft2 = 3.4 %, 3.6 % and 3.7 % with comparison to the normal sample (n = 3.0 %). Our study proved a direct, easy, and simple method of determining the degree of polarization of the polymers without the need of complex formulation and labelling protocol. Therefore, this work may enhance the investigation of the optical properties of the agarose-based tissue-mimicking phantom (AGTMP) for modeling or simulation of the real biological sample in the future. Our polarization measures are worthy of further explored and implemented in current optical imaging techniques or sensing platform for optical classification of the biomaterials.
  5. Musa SM, Sining S, Arba'in AI, Amizuri MI, Lazim AM, Ghaffar MA
    Mar Pollut Bull, 2025 Mar;212:117584.
    PMID: 39842134 DOI: 10.1016/j.marpolbul.2025.117584
    Casting a shadow upon marine ecosystems worldwide, microplastic pollution emerges as a formidable threat, yet its repercussions on commercially significant marine fish remain largely unexplored. This investigation delves into the realm of microplastic contamination within three tuna species (Euthynnus affinis, Thunnus tonggol, and Auxis rochei) inhabiting Malaysian waters, with a keen focus on the gastrointestinal tract (GIT), gills, and edible muscle. These species were chosen due to their significant ecological roles and considerable commercial value, being extensively fished and widely consumed across global regions. Scrutinising 125 tuna specimens uncovers a spectrum of contamination levels across species, with T. tonggol registering the highest concentration (1.22 ± 0.14 microplastics per gram). Remarkably, male tunas exhibit a discernible proclivity towards heightened microplastic accumulation relative to their female counterparts. Noteworthy morphotypes of microplastics predominantly encompass fibres (92.43 %), notably manifesting in black hues (63.25 %). Notably, microplastics spanning the 0.01-1.00 mm size range assert their dominance, constituting a substantial 61.54 % of the total, with rayon emerging as the leading polymer (54.39 %). These findings highlight the pivotal role of the GIT and gills as primary sites of microplastic accumulation in tuna, raising concerns about potential human health implications through seafood consumption. It is crucial to deepen our understanding of microplastic distribution and characteristics in seafood to develop effective mitigation strategies and safeguard human well-being within marine environments. This study advocates for urgent global attention to address the ecological and human health impacts of microplastic contamination in marine ecosystems.
    MeSH terms: Animals; Environmental Monitoring*; Female; Gills; Malaysia; Male; Tuna*
  6. Sulaiman IM, Bulgiba A, Kareem SA, Latip AA
    Methods Inf Med, 2025 Feb 11.
    PMID: 39842453 DOI: 10.1055/a-2521-4372
    OBJECTIVE:  This is the first Malaysian machine learning model to detect and disambiguate abbreviations in clinical notes. The model has been designed to be incorporated into MyHarmony, a natural language processing system, that extracts clinical information for health care management. The model utilizes word embedding to ensure feasibility of use, not in real-time but for secondary analysis, within the constraints of low-resource settings.

    METHODS:  A Malaysian clinical embedding, based on Word2Vec model, was developed using 29,895 electronic discharge summaries. The embedding was compared against conventional rule-based and FastText embedding on two tasks: abbreviation detection and abbreviation disambiguation. Machine learning classifiers were applied to assess performance.

    RESULTS:  The Malaysian clinical word embedding contained 7 million word tokens, 24,352 unique vocabularies, and 100 dimensions. For abbreviation detection, the Decision Tree classifier augmented with the Malaysian clinical embedding showed the best performance (F-score of 0.9519). For abbreviation disambiguation, the classifier with the Malaysian clinical embedding had the best performance for most of the abbreviations (F-score of 0.9903).

    CONCLUSION:  Despite having a smaller vocabulary and dimension, our local clinical word embedding performed better than the larger nonclinical FastText embedding. Word embedding with simple machine learning algorithms can decipher abbreviations well. It also requires lower computational resources and is suitable for implementation in low-resource settings such as Malaysia. The integration of this model into MyHarmony will improve recognition of clinical terms, thus improving the information generated for monitoring Malaysian health care services and policymaking.

  7. Alby A, Power A, Flaherty GT
    Travel Med Infect Dis, 2025;64:102803.
    PMID: 39842532 DOI: 10.1016/j.tmaid.2025.102803
    INTRODUCTION: There has been limited literature reporting the nature of the consular assistance provided to travellers. This study aimed to describe the consular assistance activity of the Irish Department of Foreign Affairs.

    METHODS: Publicly available consular assistance data (2014-2023) were analysed descriptively. Consular missions were listed under 16-19 categories.

    RESULTS: A total of 26,378 consular assistance missions were recorded. The greatest proportion of missions related to COVID-19 repatriations (26.9 %, n = 7097), deaths overseas (10 %, n = 2650), and medical illness/mental health emergency abroad (11.6 %, n = 3047). Forensic incidents (arrests/victims of crime) accounted for 12.9 % (n = 3392) of consular assistance episodes.

    CONCLUSION: This study highlights the characteristics and trends of consular assistance requests received by a national governmental agency. Future studies should explore traveller awareness of consular procedures and the extent to which they are addressed in travel medicine consultations.

    MeSH terms: Humans; International Cooperation; Ireland; Travel Medicine
  8. Bushra SMR, Safuan S, Abu Bakar R, Lima MA, Abubakar BD, Nurul AA
    Int J Biol Macromol, 2025 Apr;299:140103.
    PMID: 39842582 DOI: 10.1016/j.ijbiomac.2025.140103
    Asthma is a chronic respiratory disease characterized by airway inflammation. Lignosus rhinocerotis (LR), a medicinal mushroom rich in polysaccharide, has been traditionally used to treat various diseases, including asthma. This study aimed to fractionate, characterize and evaluate the anti-asthmatic effects of polysaccharides from LR (LRP). LRP was isolated and characterized using high-performance liquid chromatography (HPLC), x-ray diffraction analysis (XRD), fourier transform infrared spectrometry (FTIR) and nuclear magnetic resonance (NMR) spectroscopy. In an OVA-induced asthma model, BALB/c mice were sensitized, challenged, and intranasally treated with LRP. Experimental findings demonstrated that LRP exhibits a semi-crystalline nature with predicted structure of → 4)-α-D-Glcp-(1 → and → 3)-β-D-Glcp-(1→. LRP significantly reduced eosinophilia, Th2 cytokines, and IgE levels. Histological analysis revealed LRP's ability to decrease epithelial damage and epithelial and smooth muscle thickness in lung. Reductions in inflammatory cell infiltration, mucus production, and transforming growth factor (TGF)-β1 expression were observed, although not statistically significant. Gene expression analysis indicated that LRP significantly downregulated the inducible nitric oxide synthase (iNOS) expression. This study highlights a detailed structural analysis of LRP and its potential as an alternative for the management of asthma. Further research is needed to elucidate the precise mechanisms of action and optimize its therapeutic application.
    MeSH terms: Animals; Disease Models, Animal; Female; Immunoglobulin E/blood; Lung/drug effects; Lung/pathology; Mice, Inbred BALB C*; Polysaccharides/pharmacology; Polysaccharides/chemistry; Cytokines/metabolism; Mice; Nitric Oxide Synthase Type II/metabolism
  9. Mohammad Azmi N, Nadzira A, Abdul Rahman NA, Mohd Azman ZA, Chandrakanthan SB, Dualim DM, et al.
    World J Surg, 2025 Mar;49(3):576-583.
    PMID: 39843355 DOI: 10.1002/wjs.12490
    BACKGROUND: Aims conventional bowel preparation restricts dietary intake up to 72 h prior to colonoscopy. Bowel preparation process is often perceived as unpleasant leading to poor compliance and subsequent poor bowel preparation. The aim of this trial is to compare the efficacy of low-residue semi-elemental enteral formula (LREF) incorporated diet versus the standard diet in polyethylene glycol (PEG)-based bowel preparation in the aim of creating a more tolerable bowel preparation regimen without compromising bowel cleanliness.

    METHODS: This was a multicenter, prospective, single-blinded, randomized controlled noninferiority trial. The noninferiority margin was set at 15%. One hundred sixty-seven patients were recruited and randomized to either the LREF group or the standard diet (SD) group using a 3L PEG preparation regimen.

    RESULTS: The LREF group results in comparable satisfactory preparation rating to the standard diet group with a mean BPPS score of 6.87 (SD 1.59) versus 7.14 (SD 1.54) (95% CI[-0.86; 0.32] and p = 0.367). The mean difference (MD) of the BBPS total score between the two groups was -0.27 (95% CI [-0.764 and 0.224]). Equivalence were demonstrated using the two one-sided test (alpha = 5%) with the lower t-value of 2.682 (p = 0.0042) and the upper t-value of -4.493 (p 

    MeSH terms: Adult; Aged; Female; Food, Formulated; Humans; Male; Middle Aged; Patient Compliance/statistics & numerical data; Prospective Studies; Single-Blind Method
  10. Huang T, Fakurazi S, Cheah PS, Ling KH
    Sci Rep, 2025 Jan 22;15(1):2818.
    PMID: 39843579 DOI: 10.1038/s41598-025-87314-y
    Increasing shreds of evidence suggest that neurogenic-to-gliogenic shift may be critical to the abnormal neurodevelopment observed in individuals with Down syndrome (DS). REST, the Repressor Element-1 Silencing Transcription factor, regulates the differentiation and development of neural cells. Downregulation of REST may lead to defects in post-differentiation neuronal morphology in the brain of the DS fetal. This study aims to elucidate the role of REST in DS-derived NPCs using bioinformatics analyses and laboratory validations. We identified and validated vital REST-targeted DEGs: CD44, TGFB1, FN1, ITGB1, and COL1A1. Interestingly, these genes are involved in neurogenesis and gliogenesis in DS-derived NPCs. Furthermore, we identified nuclear REST loss and the neuroblast marker, DCX, was downregulated in DS human trisomic induced pluripotent stem cells (hiPSCs)-derived NPCs, whereas the glioblast marker, NFIA, was upregulated. Our findings indicate that the loss of REST is critical in the neurogenic-to-gliogenic shift observed in DS-derived NPCs. REST and its target genes may collectively regulate the NPC phenotype.
    MeSH terms: Cell Differentiation/genetics; Gene Expression Regulation; Humans; Antigens, CD44; Antigens, CD29/genetics; Antigens, CD29/metabolism; Computational Biology/methods; NFI Transcription Factors/genetics; NFI Transcription Factors/metabolism; Transforming Growth Factor beta1/genetics; Transforming Growth Factor beta1/metabolism
  11. Zakaria NH, Abd Rahim NDE, Rosilan NF, Sung YY, Waiho K, Harun S, et al.
    World J Microbiol Biotechnol, 2025 Jan 23;41(2):45.
    PMID: 39843643 DOI: 10.1007/s11274-025-04262-5
    Vibrio parahaemolyticus poses a notable threat to marine ecosystems and can cause infections and disease outbreaks in seafood species, which can affect humans upon consumption. The global impacts of such infections and outbreaks on human and animal health led to a growing number of studies from various countries discussing the prevention, control, treatment, and overall implications of V. parahaemolyticus. Hence, this study aims to retrieve relevant studies on V. parahaemolyticus using a bibliometric analysis to understand current research status, trends, and hotspots regarding this bacteria. Relevant literature was searched across the Scopus database, and the data were subsequently analyzed using Biblioshiny software. In addition, a manual examination was conducted to identify the hosts of V. parahaemolyticus and diseases caused by the bacteria. Overall, 7,096 records were obtained from Scopus from 1963 to 2023. A bibliometric analysis identified 17,220 authors, with China emerging as the global leader. The analysis also highlighted significant keywords such as "Vibrio parahaemolyticus," "Litopenaeus vannamei," and "innate immunity," suggesting a focus on the impact of V. parahaemolyticus on L. vannamei, specifically emphasizing the shrimp's innate immune responses. Host-disease interaction network also uncovered 53 interactions between hosts and diseases involving L. vannamei or Penaeus vannamei as the primary host, with acute hepatopancreas necrosis disease (AHPND) emerging as the most prevalent among them. This study can enhance our understanding of infections caused by V. parahaemolyticus and contribute to the development of effective strategies for their prevention and management.
    MeSH terms: Animals; China; Humans; Immunity, Innate; Vibrio parahaemolyticus*; Bibliometrics*; Seafood/microbiology; Penaeidae/microbiology; Host-Pathogen Interactions
  12. Lim BH, D'Ippoliti C, Dominik M, Hernández-Mondragón AC, Vermeir K, Chong KK, et al.
    Nature, 2025 Feb;638(8050):459-468.
    PMID: 39843736 DOI: 10.1038/s41586-024-08422-9
    The assessment of research performance is widely seen as a vital tool in upholding the highest standards of quality, with selection and competition believed to drive progress. Academic institutions need to take critical decisions on hiring and promotion, while facing external pressure by also being subject to research assessment1-4. Here we present an outlook on research assessment for career progression with specific focus on promotion to full professorship, based on 314 policies from 190 academic institutions and 218 policies from 58 government agencies, covering 32 countries in the Global North and 89 countries in the Global South. We investigated how frequently various promotion criteria are mentioned and carried out a statistical analysis to infer commonalities and differences across policies. Although quantitative methods of assessment remain popular, in agreement with what is found in more geographically restricted studies5-9, they are not omnipresent. We find differences between the Global North and the Global South as well as between institutional and national policies, but less so between disciplines. A preference for bibliometric indicators is more marked in upper-middle-income countries. Although we see some variation, many promotion policies are based on the assumption of specific career paths that become normative rather than embracing diversity. In turn, this restricts opportunities for researchers. These results challenge current practice and have strategic implications for researchers, research managers and national governments.
  13. Xiong Q, Chen Q, Tang S, Li Y
    Sci Rep, 2025 Jan 22;15(1):2879.
    PMID: 39843892 DOI: 10.1038/s41598-025-85721-9
    Deep learning has achieved significant success in the field of defect detection; however, challenges remain in detecting small-sized, densely packed parts under complex working conditions, including occlusion and unstable lighting conditions. This paper introduces YOLOv8-n as the core network to propose VEE-YOLO, a robust and high-performance defect detection model. Firstly, GSConv was introduced to enhance feature extraction in depthwise separable convolution and establish the VOVGSCSP module, emphasizing feature reusability for more effective feature engineering. Secondly, improvements were made to the model's feature extraction quality by encoding inter-channel information using efficient multi-Scale attention to consider channel importance. Precise integration of spatial structural and channel information further enhanced the model's overall feature extraction capability. Finally, EIoU Loss replaced CIoU Loss to address bounding box aspect ratio variability and sample imbalance challenges, significantly improving overall detection task performance. The algorithm's performance was evaluated using a dataset to detect stranded elastic needle defects. The experimental results indicate that the enhanced VEE-YOLO model's size decreased from 6.096 M to 5.486 M, while the detection speed increased from 179FPS to 244FPS, achieving a mAP of 0.926. Remarkable advancements across multiple metrics make it well-suited for deploying deep detection models in complex industrial environments.
  14. Abdullah N, Goh YX, Khuzaimi AN, Kamalul Arifin AS, Mhd Yusuf NA, Abd Jalal N, et al.
    J Diabetes, 2025 Jan;17(1):e70039.
    PMID: 39843936 DOI: 10.1111/1753-0407.70039
    BACKGROUND: Emerging evidence suggests that the creatinine-to-body weight (Cre/BW) ratio is a predictor for incident diabetes in the Asian population. This study examined the association between Cre/BW ratio and incident diabetes, as well as the relationship between Cre/BW ratio and skeletal muscle and body fat mass in a multiethnic Malaysian cohort.

    METHODS: A total of 13 047 eligible participants were selected from 119 560 The Malaysian Cohort participants. Of these, 750 who developed diabetes were selected as cases, while 3750 controls were chosen randomly from healthy participants. This nested case-control study included 4500 eligible participants from The Malaysian Cohort, with a 1:5 case-to-control ratio. Participants were stratified into four groups based on Cre/BW ratio quartiles. The Cox proportional hazards model evaluated the effect of Cre/BW ratio on developing incident diabetes. The association between Cre/BW ratio and body composition was assessed using the Pearson correlation coefficient.

    RESULTS: Of the 13 047 eligible participants followed up over 5.3 years, 5.75% (n = 750) developed diabetes. Diabetes incidence decreased with increasing Cre/BW ratios. The Cre/BW ratio was inversely correlated with diabetes risk (HR: 0.403, 95% CI: 0.315-0.515, p 

    MeSH terms: Adult; Aged; Body Composition; Body Weight*; Female; Humans; Malaysia/epidemiology; Male; Middle Aged; Risk Factors; Cohort Studies; Body Mass Index; Incidence; Case-Control Studies
  15. Shabil M, Gaidhane S, Roopashree R, Kaur M, Srivastava M, Barwal A, et al.
    BMC Infect Dis, 2025 Jan 22;25(1):102.
    PMID: 39844097 DOI: 10.1186/s12879-025-10512-6
    BACKGROUND: Mpox is a viral zoonotic disease that has seen a resurgence in recent years, with outbreaks reaching beyond its traditional endemic zones in Central and West Africa to parts of Europe and North America. The relationship between human immunodeficiency virus (HIV) infection and mpox outcomes, particularly hospitalization rates, remains underexplored despite the known immunosuppressive effects of HIV. This systematic review and meta-analysis aimed to clarify the association between HIV infection and the likelihood of hospitalization in mpox cases.

    METHODS: A literature search was conducted through PubMed, Embase, Web of Science, Scopus, and the Cochrane Library up until August 10, 2024. The eligibility criteria focused on observational studies that evaluated hospitalization rates among mpox cases, distinguishing between HIV-positive and HIV-negative individuals. Newcastle-Ottawa Scale was used for evaluating study quality. The meta-analysis used a random-effects model to accommodate expected study heterogeneity using R software (V. 4.4).

    RESULTS: The search yielded 686 records, with 14 studies meeting the inclusion and exclusion criteria after screenings and full-text assessments. The pooled analysis revealed a 56.6% increased risk of hospitalization among HIV-positive mpox cases compared to HIV-negative individuals (95% CI: 18.0-107.7%). Notable heterogeneity (I² = 76%) was observed, likely reflecting variations in study settings and methodologies. Sensitivity analysis confirmed the robustness of these findings, and no significant publication bias was detected (Egger's test p-value = 0.733).

    CONCLUSION: HIV infection is associated with a statistically significant increased risk of hospitalization in mpox cases. There is a critical need for integrated care and enhanced surveillance, especially in populations with high HIV prevalence. Our findings emphasize the importance of ongoing research to better understand HIV and mpox co-infection and to refine management strategies for this vulnerable group. Future studies should focus on long-term outcomes and the effectiveness of various management strategies across different healthcare settings.

    MeSH terms: Animals; Humans; Zoonoses/epidemiology; Zoonoses/virology
  16. Hussain ASM, Ghadzi SMS, Sulaiman SAS, Alsahali SM, Khan SF
    J Health Popul Nutr, 2025 Jan 22;44(1):15.
    PMID: 39844331 DOI: 10.1186/s41043-025-00751-3
    BACKGROUND: Medication reconciliation has been acknowledged as a key intervention against medication errors. More than half of the medication errors that happen during care transitions are caused by unjustified medication discrepancies and up to one-third of these mistakes may be harmful. The study aimed to evaluate the knowledge, attitude and practices (KAP) of health care providers in on medication reconciliation process, pre and post educational intervention.

    METHODS: A hospital-based prospective quasi-experimental pre-post intervention study was conducted from November 2023 to February 2024 among 346 healthcare professionals (medical doctors, pharmacists and nurses) practicing in King Saud Hospital (KSH), Unayzah, Saudi Arabia. The subjects were recruited using the convenience sampling method.An educational intervention workshop was conducted among the healthcare professionals. The KAP was assessed before and after the educational intervention using a content and face validated self-administered questionnaire. The statistical analyses were conducted using the Statistical Package for Social Sciences (SPSS). Wilcoxon Signed Rank Test was used to differentiate the mean response scores for knowledge, attitude, and practice domains, between pre and post intervention. Kruskal Wallis Test followed by Dunn's post hoc test was used to compare the mean response scores for knowledge, attitude and practice domains based on educational qualification and practicing experience for the pre and post-test period. The level of significance is determined at P 

    MeSH terms: Adult; Attitude of Health Personnel; Female; Health Personnel/education; Humans; Health Knowledge, Attitudes, Practice*; Male; Middle Aged; Prospective Studies; Surveys and Questionnaires; Saudi Arabia
  17. Barker JN, Casanova E, Choon SE, Foley P, Fujita H, Gonzalez C, et al.
    Br J Dermatol, 2025 Mar 18;192(4):706-716.
    PMID: 39844356 DOI: 10.1093/bjd/ljae491
    BACKGROUND: Generalized pustular psoriasis (GPP) is a chronic, systemic, neutrophilic inflammatory disease. A previous Delphi panel established areas of consensus on GPP, although patient perspectives were not included and aspects of treatment goals remained unclear.

    OBJECTIVES: To identify and achieve consensus on refined, specific treatment goals for GPP treatment via a Delphi panel with patient participation.

    METHODS: Statements were generated based on a systematic literature review and revised by a Steering Committee. Statements were categorized into overarching principles, and short- and long-term treatment goals. A global panel of 30 dermatologists and 3 patient representatives voted in agreement or disagreement with each statement. Consensus was defined as ≥ 80% approval by the panellists.

    RESULTS: Consensus was reached in the first round of voting and ≥ 90% agreement was reached for 23 of 26 statements. In summary, GPP requires a timely, tailored treatment plan, co-developed by patients and physicians, that involves a multidisciplinary approach and addresses the complexity, heterogeneity and chronicity of the disease. Short-term treatment goals should include pustule clearance within 7 days and prevention of pustule recurrence, reduction of cutaneous symptom burden (-4 or more points on the Itch and Skin Pain Numeric Rating Scale), improvement in systemic symptoms (e.g. resolution of fever within 3 days of treatment initiation and reduced fatigue), prevention of life-threatening complications and progressive improvement of inflammatory biomarkers. In patients with comorbid psoriatic diseases, treatment decisions should prioritize GPP. Long-term treatment goals should include minimizing disease activity through flare prevention and symptom control between flares, sustained disease control, management of comorbidities and improvement in quality of life (QoL). Small differences in perception between patients and physicians regarding the importance of certain treatment goals (e.g. avoiding hair and/or nail loss to improve QoL), reflect the complexity of assessing treatment goals and emphasize the need for a patient-centred approach.

    CONCLUSIONS: In the first global Delphi panel in GPP to include patient perspectives, consensus between dermatologists and patients was achieved on overarching principles of treatment, and short- and long-term treatment goals for GPP. These findings provide valuable insights for developing guidelines that consider the perspectives of patients and physicians in the treatment of GPP.

    MeSH terms: Delphi Technique*; Dermatologic Agents/therapeutic use; Humans; Patient Care Planning/standards; Patient Participation; Quality of Life; Consensus*
  18. Kambach S, Bruelheide H, Comita LS, Condit R, Wright SJ, Aguilar S, et al.
    Ecology, 2025 Jan;106(1):e4527.
    PMID: 39844593 DOI: 10.1002/ecy.4527
    All species must partition resources among the processes that underly growth, survival, and reproduction. The resulting demographic trade-offs constrain the range of viable life-history strategies and are hypothesized to promote local coexistence. Tropical forests pose ideal systems to study demographic trade-offs as they have a high diversity of coexisting tree species whose life-history strategies tend to align along two orthogonal axes of variation: a growth-survival trade-off that separates species with fast growth from species with high survival and a stature-recruitment trade-off that separates species that achieve large stature from species with high recruitment. As these trade-offs have typically been explored for trees ≥1 cm dbh, it is unclear how species' growth and survival during earliest seedling stages are related to the trade-offs for trees ≥1 cm dbh. Here, we used principal components and correlation analyses to (1) determine the main demographic trade-offs among seed-to-seedling transition rates and growth and survival rates from the seedling to overstory size classes of 1188 tree species from large-scale forest dynamics plots in Panama, Puerto Rico, Ecuador, Taiwan, and Malaysia and (2) quantify the predictive power of maximum dbh, wood density, seed mass, and specific leaf area for species' position along these demographic trade-off gradients. In four out of five forests, the growth-survival trade-off was the most important demographic trade-off and encompassed growth and survival of both seedlings and trees ≥1 cm dbh. The second most important trade-off separated species with relatively fast growth and high survival at the seedling stage from species with relatively fast growth and high survival ≥1 cm dbh. The relationship between seed-to-seedling transition rates and these two trade-off aces differed between sites. All four traits were significant predictors for species' position along the two trade-off gradients, albeit with varying importance. We concluded that, after accounting for the species' position along the growth-survival trade-off, tree species tend to trade off growth and survival at the seedling with later life stages. This ontogenetic trade-off offers a mechanistic explanation for the stature-recruitment trade-off that constitutes an additional ontogenetic dimension of life-history variation in species-rich ecosystems.
    MeSH terms: Ecuador; Malaysia; Panama; Population Dynamics; Taiwan; Trees/growth & development; Trees/physiology; Tropical Climate*; Forests*
  19. Buhari SB, Ghahremani Nezhad N, Normi YM, Mohd Shariff F, Leow TC
    J Biomol Struct Dyn, 2025 Jan 23.
    PMID: 39844700 DOI: 10.1080/07391102.2024.2440646
    The quest for sustainable solutions to plastic pollution has driven research into plastic-degrading enzymes, offering promising avenues for polymer recycling applications. However, enzymes derived from natural sources often exhibit suboptimal thermostability, hindering their industrial viability. Protein engineering techniques have emerged as a powerful approach to enhance the desired properties of these biocatalysts. This study aims to conduct a comprehensive analysis of the thermostability of Vibrio palustris PETase (VpPETase) through an integrated computational approach encompassing homology modeling, site-specific molecular docking, molecular dynamics (MD) simulations, and comparative evaluation of a single-point mutation (V195F) against the wild-type enzyme. Homology modeling was used to predict VpPETase model using multiple templates. Model quality was rigorously assessed using Ramachandran plot analysis, ProSA, Verify 3D, and ERRAT. Molecular docking elucidated the catalytic region comprising residues His149, Asp117, and Ser71, while highlighting the pivotal roles of His149, Tyr1, and Ser71 in substrate binding affinity. MD simulations at various temperatures revealed higher stability at 313.15 K over a 100 ns trajectory, as evidenced by analyses of root-mean-square deviation (RMSD), radius of gyration (Rg), solvent-accessible surface area (SASA), hydrogen bonding, and root-mean-square fluctuation (RMSF). The V195F mutant exhibited a slight increase in stability compared to wild-type. While this study provides valuable insights into the thermostability of VpPETase, further investigations, including experimental validation of thermostability enhancements and in vitro characterization, are warranted to fully exploit the potential of this enzyme for industrial applications in plastic recycling.
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