Browse publications by year: 2025

  1. Kumar RNH, Rao CR, Maradi R, Umakanth S, Sanju SVC, Balu PS
    Perspect Clin Res, 2025;16(1):31-37.
    PMID: 39867528 DOI: 10.4103/picr.picr_20_24
    AIM: The study aimed to determine the incidence of adverse drug reactions (ADRs) among newly diagnosed tuberculosis (TB) patients receiving daily drug regimen with fixed-dose combination treatment under the National Tuberculosis Elimination Program.

    MATERIALS AND METHODS: A community-based prospective cohort study was carried out in the Udupi district. Over 12 months, all newly diagnosed TB patients of either gender were included from 63 primary health centers and 6 community health centers, and ADRs were recorded by personal interviews.

    RESULTS: A total of 710 patients were enrolled, among whom 453 (63.8%), were males, and 257 (36.2%) were females. Pulmonary TB was diagnosed among 510 (71.8%) and 200 (28.2%) were extrapulmonary cases. During the intensive phase (IP) of treatment, 480 (67.6%) patients reported at least one ADR and 79 (11.1%) experienced two ADRs during IP and 31 (6.5%) had ADRs during the continuation phase. Out of 480, 140 (29.2%) had gastritis, 132 (27.5%) had vomiting, 105 (21.9%) had nausea, 60 (12.5%) had skin rashes, 27 (5.6%) had drug-induced hepatitis, and 16 (3.3%) had vision problems. Among 480 patients with ADRs, 462 (96.3%) had successful treatment outcomes, the remaining 17 patients (3.5%) died, and one (0.2%) had treatment failure.

    CONCLUSIONS: Adverse events were more common in the 1st few months of treatment than in subsequent months. All mild-to-moderate ADRs were effectively managed, and most had successful treatment outcomes.

  2. Arshed M, Mahmud AB, Umer MF, Mashhadi F, Kawish AB
    Pak J Med Sci, 2025 Jan;41(1):22-28.
    PMID: 39867791 DOI: 10.12669/pjms.41.1.9272
    BACKGROUND & OBJECTIVES: Poor medication adherence is an essential contributor to Pakistan's high prevalence of uncontrolled hypertension. This study will be aimed to assess the efficacy of a one-of-a-kind developed intervention in improving medication adherence and treatment outcomes in hypertension patients.

    METHODS: Twleve months duration long randomized controlled trial from January to December 2021 will be carried out at Shaikh Zayed Medical Complex (SZMC), Lahore. A total of 440 patients aged 18 years and older diagnosed with hypertension in the last month with non- adherence to antihypertensive therapy <80% of pills used in the last 30 days and have access to a smartphone will be randomized into either the intervention group (n=220) or the control group (n=220). For the intervention arm, a comprehensive intervention, the "Multi-Aid-Package," consisting of seven items: written, voice, and graphics messages, animated video, educational material, and a 24/7 help service, has been designed. Standard care will be provided to the control group. The primary outcome will be improved adherence to antihypertensive medication, while the secondary outcome will be an alteration in systolic blood pressure (SBP). The analysis will be intention to treat.

    CONCLUSION: According to this study, if the multifunctional Multi-Aid-Package proves to be a useful mobile health tool for improving hypertension patients' medication adherence, it will also significantly affect systolic blood pressure. In Pakistan and other comparable low- and middle-income countries LMICs, the Multi-Aid-Package ought to be taken into consideration as a means of enhancing adherence to medications among hypertension patients.

  3. Bushi G, Balaraman AK, Gaidhane S, Ballal S, Kumar S, Bhat M, et al.
    Brain Behav Immun Health, 2025 Feb;43:100931.
    PMID: 39867846 DOI: 10.1016/j.bbih.2024.100931
    BACKGROUND AND OBJECTIVE: Lyme disease, caused by Borrelia burgdorferi, presents major health challenges worldwide, leading to serious neurological and musculoskeletal issues that impact patients' lives and healthcare systems. This systematic review and meta-analysis aim to determine the prevalence and link between Lyme disease and these complications, aiming to enhance clinical and public health approaches.

    METHODS: We systematically searched PubMed, EMBASE, and Web of Science up until April 01, 2024, to find studies reporting the prevalence and severity of neurological and musculoskeletal complications associated with Lyme disease. Screening and data extraction were conducted using Nested Knowledge software. Two independent reviewers performed the quality assessment using the Newcastle-Ottawa Scale. Meta-analyses were performed using R software v4.3, employing a random-effects model.

    RESULTS: Out of 3576 records, 17 studies were included, involving 3932 participants. These studies revealed significant prevalence of musculoskeletal symptoms (21.1%) and neurological disabilities (18%) among Lyme disease patients. The analysis showed a notable increase in risk for both complications in individuals with Lyme disease, with pooled Risk Ratios (RR) of 1.82 for musculoskeletal symptoms and 1.64 for neurological disabilities, indicating a significantly higher risk compared to control groups. Although heterogeneity across the studies was high, sensitivity analysis confirmed the consistency of our findings. Additionally, there was evidence of publication bias.

    CONCLUSION: The study reveals significant neurological and musculoskeletal complications in Lyme disease patients, emphasizing the importance of early diagnosis, comprehensive treatment, and supportive care. The noted heterogeneity and potential publication bias highlight the need for transparent research and further study on long-term outcomes.

  4. Bashari MH, Agung MUK, Ariyanto EF, Al Muqarrabun LMR, Salsabila S, Chahyadi A, et al.
    J Exp Pharmacol, 2025;17:37-50.
    PMID: 39867869 DOI: 10.2147/JEP.S494777
    PURPOSE: A promising feature of marine sponges is the potential anticancer efficacy of their secondary metabolites. The objective of this study was to explore the anticancer activities of compounds from the fungal symbiont of Aaptos suberitoides on breast cancer cells.

    METHODS: In the present research, Aspergillus unguis, an endophytic fungal strain derived from the marine sponge A. suberitoides was successfully isolated and characterized. Subsequently, ethyl acetate extraction and isolation of chemical constituents produced was performed. The structures of the isolated compounds were identified using several spectroscopic methods, ie, UV, NMR, and mass spectrometry. Thereafter, MDA-MB-231, MCF-7 breast cancer cells and HaCat cells were treated with the isolated compounds. Not only viability, apoptosis, and cell cycle analyses were conducted, but also the mRNA expression of MCL1, BCL2L1, AKT1 and CDK2 were evaluated.

    RESULTS: The extract showed cytotoxic activity in breast cancer cells. Two novel compounds were successfully isolated and identified, ie, Unguisol A (15.1 mg) and Unguisol B (97.9 mg). Both compounds share the same basic skeleton and comprise an aromatic ring which is attached to a sulphur-containing, seven-membered ring via an oxygen atom. This marked the first-time isolation of Unguisol A and Unguisol B from A. unguis, highlighting their novelty. Both compounds induced early apoptosis (p < 0.01) and cell cycle arrest at the S phase (p < 0.05) in MDA-MB-231 cells, but not in HaCat cells. Both compounds suppressed BCL2L1 and AKT1 mRNA expression (p < 0.01).

    CONCLUSION: Two novel compounds were isolated from A. unguis. Unguisol A and Unguisol B induced apoptosis in MDA-MB-231 breast cancer cells via BCL2L1 mRNA downregulation, while both compounds induced cell cycle arrest at the S phase through AKT1 mRNA downregulation.

  5. Zahedi A, Katembu S, Sind SM, Sommer U, Kimamo C, Sommer W
    Appetite, 2025 Feb 01;206:107839.
    PMID: 39736414 DOI: 10.1016/j.appet.2024.107839
    The ongoing obesity epidemic is an indicator that traditional efforts towards diet change are insufficient, and interventions focusing mainly on restrictions of unhealthy food are of limited success. Therefore, approaches targeting food preferences should be integral in counteracting the current epidemic. However, food preferences are affected by a multitude of factors and are usually resistant to changes later in life. Hence, we tested whether the food choices of preschool children can be changed by a child-appropriate, interactive, fairytale-like narrative. We used two interactive stories: the first (experimental) story was about how two protagonists of similar age as the participants saved their hometown from being drained of color with the help of magic fruits or vegetables, while the second (control) story consisted of a similar plot that did not mention food. In Experiment 1, we used a crossover design with a one-week baseline measurement. After the experimental stories, healthy food choices (i.e., pieces of fruit vs. cookies, sweets, or cake) increased significantly relative to the one-week baseline, whereas no change was observed after the control story. In preregistered Experiment 2, we replicated these results with vegetables as healthy options using a random control design and investigated the longevity of the effects. The food-story effect on vegetable choices was similar to fruits (Exp. 1) and lasted for two weeks. These findings demonstrate that appropriate narratives about food can have a strong and lasting effect on the healthy food choices of preschool children and might promote healthy food consumption if incorporated into nutrition education.
    MeSH terms: Child, Preschool; Choice Behavior*; Female; Fruit; Humans; Male; Vegetables*; Cross-Over Studies; Narration; Healthy Diet/psychology
  6. Joshi DC, Chavan MB, Gurow K, Gupta M, Dhaliwal JS, Ming LC
    Biomed Pharmacother, 2025 Feb;183:117827.
    PMID: 39854819 DOI: 10.1016/j.biopha.2025.117827
    Huntington's disease (HD) is a progressive, autosomal dominant neurodegenerative disorder characterized by cognitive decline, motor dysfunction, and psychiatric disturbances. A common feature of neurodegenerative disorders is mitochondrial dysfunction, which affects the brain's sensitivity to oxidative damage and its high oxygen demand. This dysfunction may plays a significant role in the pathogenesis of Huntington's disease. HD is caused by a CAG repeat expansion in the huntingtin gene, which leads to the production of a toxic mutant huntingtin (mHTT) protein. This disruption in mitochondrial function compromises energy metabolism and increases oxidative stress, resulting in mitochondrial DNA abnormalities, impaired calcium homeostasis, and altered mitochondrial dynamics. These effects ultimately may contribute to neuronal dysfunction and cell death, underscoring the importance of targeting mitochondrial function in developing therapeutic strategies for HD. This review discusses the mechanistic role of mitochondrial dysfunction in Huntington's disease. Mitochondrial dysfunction is a crucial factor in HD, making mitochondrial-targeted therapies a promising approach for treatment. We explore therapies that address bioenergy deficits, antioxidants that reduce reactive oxygen species, calcium modulators that restore calcium homeostasis, and treatments that enhance mitochondrial dynamics to rejuvenate mitochondrial function. We also highlight innovative treatment approaches such as gene editing and stem cell therapy, which offer hope for more personalized strategies. In conclusion, understanding mitochondrial dysfunction in Huntington's disease may guide potential treatment strategies. Targeting this dysfunction may help to slow disease progression and enhance the quality of life for individuals affected by Huntington's disease.
    MeSH terms: Animals; Energy Metabolism/drug effects; Humans; Reactive Oxygen Species/metabolism; Oxidative Stress
  7. Yu X, Lim KS, Tang LY, David P, Ong ZQ, Wong KY, et al.
    Epilepsy Behav, 2025 Jan 23;164:110262.
    PMID: 39854827 DOI: 10.1016/j.yebeh.2025.110262
    OBJECTIVES: Caregiving for adults with epilepsy (AWE) imposes a different degree and scope of challenges than for children and teenagers with epilepsy, and it remains understudied. This study aimed to identify the types of caregiver burdens, needs, and coping strategies in caregiving for AWE.

    METHODS: Previous studies relevant to this topic were identified from 7 databases. Searches were performed in PubMed, CINAHL, Web of Science (WOS), Scopus, Psychology and Behavioral Science Collection (PBSC), Embase, PsychINFO from 1 Jan 1980 to 31 December 2023. The findings were reported according to caregiver burden, caregiver needs, and coping strategies.

    RESULTS: 22 studies were included in this review, including 14 quantitative and 8 qualitative studies. Six types of burdens were identified: psychological, physical, medical, economic, social, and family. The predominantly reported burden was the psychological burden in developed and developing countries. However, caregivers in developing countries reported additional physical, social, and economic burdens due to cultural and socioeconomic factors. Furthermore, inaccessible and unaffordable health care with a lack of medical knowledge exacerbated the challenges. Stigma and misconceptions led to more psychological distress and social restriction. There is a need for psychological, social, and family support as well as medical information to promote self-efficacy among caregivers. Most caregivers develop their own coping strategies for dealing with caregiving stress.

    CONCLUSION: The caregiving burden for AWE is an understudied area that needs to be addressed by healthcare professionals. The caregivers are experiencing unaddressed psychological and other types of burdens, together with a lack of appropriate interventions and healthcare information.

  8. Alkhadher SAA, Sidek LM, Zakaria MP, Al-Gradi M, Suratman S, Khan MSJ, et al.
    Aquat Toxicol, 2025 Feb;279:107254.
    PMID: 39854961 DOI: 10.1016/j.aquatox.2025.107254
    This review article provides a thorough examination of an interaction between linear alkylbenzenes (LABs) and ecosystems. The review covers various aspects of LABs' impact on ecosystems, focusing on detection and treatment strategies to mitigate ecological consequences. It delves into LABs' role as molecular markers for sewage pollution, their physicochemical properties contributing to persistence, and their effects on aquatic and terrestrial organisms, including disruptions to endocrine systems. The diverse sources of LABs, including domestic wastewater and industrial effluents, are explored, along with their ratios in different matrices for assessing contamination origins. Biodegradation pathways of LABs, both aerobic and anaerobic, are scrutinized, considering their interaction with microbes. Distribution patterns in aquatic environments are discussed, encompassing sediment, water, sewage, and soils. An investigation is conducted on the relationship between LABs and total organic carbon (TOC) as a means of evaluating sewage pollution. It is assessed how sewage treatment facilities (STPs) contribute to biodegradation.
    MeSH terms: Animals; Benzene Derivatives/analysis; Biodegradation, Environmental*; Environmental Monitoring/methods; Sewage/chemistry; Ecosystem*; Environmental Restoration and Remediation; Waste Water/chemistry
  9. Karmakar S, Riya KK, Jolly YN, Akter S, Mamun KM, Kabir J, et al.
    Mar Pollut Bull, 2025 Mar;212:117587.
    PMID: 39855060 DOI: 10.1016/j.marpolbul.2025.117587
    The pervasive and escalating issue of toxic metal pollution has gathered global attention, necessitating the exploration of innovative ecological strategies like phytoremediation. This study explored the extent of potentially toxic metal contamination status and the effectiveness of three planted mangrove species (Avicennia marina, Bruguiera gymnorhiza,and Excoecaria agallocha) in phytoremediation efforts to reduce pollution level. The results indicated that the mean concentrations of elements in the sediment of the area followed a descending sequence: Fe (27,136.67 ± 929.57 mg/kg) > Ti (3371.53 ± 228.08 mg/kg) > Sr (198.59 ± 37.43 mg/kg) > Zr (159.49 ± 22.35 mg/kg) > Rb (159.11 ± 17.63 mg/kg) > Cu (82.73 ± 5.01 mg/kg) > Zn (61.29 ± 2.42 mg/kg). The comprehensive assessment of pollution indices, encompassing enrichment factor (EF), contamination factor (CF), pollution load index (PLI), and geo-accumulation index (Igeo), elucidated a low to medium contamination level, particularly regarding Cu, primarily attributed to anthropogenic sources. Correlation analysis and principal component analysis (PCA) unveiled the influence of anthropogenic activities on heavy metal distribution. Evaluating the phytoremediation potentiality via bioconcentration factor (BCF) and translocation factor (TF) revealed limited metal accumulation in plant tissues, yet TF values exceeding 1 demonstrated efficient metal translocation from roots to aerial parts. Remarkably, Excoecaria agallocha exhibited the greatest phytoremediation potential, effectively translocating metals such as Cu and Zn to aerial parts (TF > 1). Thus, the intricate interplay between mangrove species and their environmental setting emerges as pivotal in curbing heavy metal transfer to neighboring estuarine and marine ecosystems.
    MeSH terms: Biodegradation, Environmental*; Environmental Monitoring; Metals; Ships; Water Pollutants, Chemical*; Geologic Sediments/chemistry; Metals, Heavy/analysis; Avicennia; Rhizophoraceae; Environmental Restoration and Remediation/methods; Wetlands*
  10. Tang L, Ng KT, Hamdan M, Awang R
    J Oral Maxillofac Surg, 2025 Apr;83(4):456-458.
    PMID: 39855628 DOI: 10.1016/j.joms.2024.12.013
    Following surgical treatment of a jaw cyst, bony cavities are formed. These cavities are prone to infection when they communicate with the oral environment. Hence, proper irrigation is a crucial aspect of postsurgical care, as it helps to reduce debris buildup and the risk of infection. However, the irrigation method of using a cannula is ineffective due to poor patient compliance. In response, we present a novel solution to this problem.
    MeSH terms: Equipment Design; Humans; Cannula
  11. Amer Nordin A, Jawahir S, Manual A, Ab Hamid J, Ab Rahim I, Mohd Noh SN, et al.
    BMJ Open, 2025 Jan 23;15(1):e081828.
    PMID: 39855658 DOI: 10.1136/bmjopen-2023-081828
    OBJECTIVES: Multimorbidity has been recognised as a global public health issue, and individuals with multimorbidity have been found to have high healthcare utilisation. This study aims to estimate the prevalence of non-communicable diseases among adults in Malaysia, identify factors associated with multimorbidity, and assess the association between the number of non-communicable diseases and outpatient services utilisation.

    DESIGN AND SETTING: A retrospective secondary data analysis using data from the National Health and Morbidity Survey 2019, a cross-sectional household survey among the population in Malaysia.

    PARTICIPANTS: All adults aged 18 years and above.

    OUTCOME MEASURES: The two outcome variables were multimorbidity and outpatient services utilisation. Characteristics of respondents and those having multimorbidity were described using complex sample descriptive statistics. We used multivariable logistic regression to determine the associated factors of having multimorbidity and the association between the number of non-communicable diseases and outpatient services utilisation.

    RESULTS: Overall, 11 347 respondents were included in the analysis. This study found a prevalence of 11.4 (95% CI=10.43-12.39) for multimorbidity. Age, marital status and working status were the factors associated with multimorbidity. Adults with multimorbidity were high users of outpatient services (20.4%, 95% CI=17.5-23.7), approximately threefold of adults with no non-communicable diseases. In the final model, multimorbidity showed an adjusted OR of 3.28 (95% CI=2.48-4.32) for outpatient services utilisation.

    CONCLUSION: Understanding factors associated with multimorbidity and the magnitude of the impact of having multimorbidity towards outpatient services utilisation could help in future planning for healthcare system transformation. The recently launched Health White Paper for Malaysia has emphasised primary healthcare as a critical component to achieve aspirations of the health system, which includes equity and responsiveness. Strengthening primary care services and improving patient navigation across healthcare levels are critical to supporting individuals with multimorbidity. Avenues for further research include exploring a wider range of conditions and assessing the longitudinal impact of multimorbidity on healthcare utilisation and health outcomes.

    MeSH terms: Adolescent; Adult; Aged; Cross-Sectional Studies; Female; Humans; Malaysia/epidemiology; Male; Middle Aged; Retrospective Studies; Prevalence; Young Adult
  12. Khan B, Batool S, Shahnawaz S, Ullah S, Naeem M, Habiba U, et al.
    Vet Parasitol Reg Stud Reports, 2025 Jan;57:101147.
    PMID: 39855880 DOI: 10.1016/j.vprsr.2024.101147
    Ticks are the second most important hematophagous ectoparasites after mosquitoes and serve as vectors for various pathogens, transmitting them to wild and domestic animals, as well as humans. Argas persicus are the soft ticks that are known to parasitize domestic poultry. Hard ticks are known to be the reservoir of Toxoplasma (T.) gondii but limited such information is available for soft ticks. This study reports the molecular prevalence and phylogenetic analysis of T. gondii infecting Argas (A.) persicus ticks (N = 70), collected from four domestic poultry breeds in the Kurram District, Khyber Pakhtunkhwa, Pakistan, between June and August 2023. The results showed that 26 out of 70 (37 %) A. persicus samples tested positive for the ITS-1 gene of T. gondii. These findings were confirmed by the DNA sequencing and BLAST analysis of amplified amplicons. Phylogenetic analysis showed that Pakistani isolates were genetically diverse and clustered with isolates that were reported from birds and mammals from Pakistan, Tunisia, Malaysia, Brazil and Iraq. Risk factor analysis indicated that the prevalence of T. gondii was not associated with any specific poultry breed, sampling period, or the body temperature of the fowl hosts. Similarly parasite prevalence was not found associated with tick sex or their developmental stage but parasite infection rate varied with the feeding status of the ticks; highest T. gondii infection rate was observed in fully fed Argas persicus. In conclusion, this is the first report from Pakistan highlighting a high prevalence of T. gondii in A. persicus infesting domestic poultry suggesting a potential zoonotic risk to humans through the consumption of infected poultry. This data is confirming that in addition to oral ingestion of oocysts, T. gondii can be mechanically transmitted through soft ticks.
    MeSH terms: Animals; Female; Pakistan/epidemiology; Phylogeny*; Tick Infestations/epidemiology; Tick Infestations/parasitology; Tick Infestations/veterinary; Prevalence; Argas
  13. Liew CH, Ong SQ, Ng DC
    Sci Rep, 2025 Jan 24;15(1):3131.
    PMID: 39856094 DOI: 10.1038/s41598-024-80538-4
    The COVID-19 pandemic has burdened healthcare systems globally. To curb high hospital admission rates, only patients with genuine medical needs are admitted. However, machine learning (ML) models to predict COVID-19 hospitalization in Asian children are lacking. This study aimed to develop and validate ML models to predict pediatric COVID-19 hospitalization. We collected secondary data with 2200 patients and 65 variables from Malaysian aged 0 to 12 with COVID-19 between 1st February 2020 and 31st March 2022. The sample was partitioned into training, internal, and external validation groups. Recursive Feature Elimination (RFE) was employed for feature selection, and we trained seven supervised classifiers. Grid Search was used to optimize the hyperparameters of each algorithm. The study analyzed 1988 children and 30 study variables after data were processed. The RFE algorithm selected 12 highly predicted variables for COVID-19 hospitalization, including age, male sex, fever, cough, rhinorrhea, shortness of breath, vomiting, diarrhea, seizures, body temperature, chest indrawing, and abnormal breath sounds. With external validation, Adaptive Boosting was the highest-performing classifier (AUROC = 0.95) to predict COVID-19 hospital admission in children. We validated AdaBoost as the best to predict COVID-19 hospitalization among children. This model may assist front-line clinicians in making medical disposition decisions.
    MeSH terms: Machine Learning*; Algorithms; Child; Child, Preschool; Female; Hospitalization*; Humans; Infant; Infant, Newborn; Malaysia/epidemiology; Male
  14. Hussain Q, Noor ASM, Qureshi MM, Li J, Atta-Ur Rahman, Bakry A, et al.
    Sci Rep, 2025 Jan 24;15(1):3113.
    PMID: 39856169 DOI: 10.1038/s41598-025-86608-5
    The Internet of Vehicles (IoV) transforms the automobile industry through connected vehicles with communication infrastructure that improves traffic control, safety and information, and entertainment services. However, some issues remain, like data protection, privacy, compatibility with other protocols and systems, and the availability of stable and continuous connections. Specific problems are related to energy consumption for transmitting information, distributing energy loads across the vehicle's sensors and communication units, and designing energy-efficient approaches to processing received data and making decisions in the context of the IoV environment. In the realm of IoV, we propose OptiE2ERL, an advanced Reinforcement Learning (RL) based model designed to optimize energy efficiency and routing. Our model leverages a reward matrix and the Bellman equation to determine the optimal path from source to destination, effectively managing communication overhead. The model considers critical parameters such as Remaining Energy Level (REL), Bandwidth and Interference Level (BIL), Mobility Pattern (MP), Traffic Condition (TC), and Network Topological Arrangement (NTA), ensuring a comprehensive approach to route optimization. Extensive simulations were conducted using NS2 and Python, demonstrating that OptiE2ERL significantly outperforms existing models like LEACH, PEGASIS, and EER-RL across various performance metrics. Specifically, our model extends the network lifetime, delays the occurrence of the first dead node, and maintains a higher residual energy rate. Furthermore, OptiE2ERL enhances network scalability and robustness, making it a superior choice for IoV applications. The simulation results highlight the effectiveness of our model in achieving energy-efficient routing while maintaining network performance under different scenarios. By incorporating a diverse set of parameters and utilizing RL techniques, OptiE2ERL provides a robust solution for the challenges faced in IoV networks. This research contributes to the field by presenting a model that optimizes energy consumption and ensures reliable and efficient communication in dynamic vehicular environments.
  15. De Angelis N, Pesce P, Kassim ZH, Yumang C, Baldi D, Menini M
    Clin Oral Investig, 2025 Jan 25;29(1):85.
    PMID: 39856380 DOI: 10.1007/s00784-025-06173-z
    OBJECTIVES: Successful implant therapy must also ensure the integration of the soft tissues around the crown/abutment emergence profile. The existing literature does not consistently agree on the necessity of a minimal amount of keratinized tissue (KT), though it appears advantageous for the long-term stability and aesthetics of implants. The purpose of this clinical retrospective study is to compare the effectiveness of amnion/chorion membrane and autogenous subepithelial connective tissue in increasing the keratinized mucosa and maintaining it over a 5-year follow-up.

    METHODS: Twenty patients who had previously undergone implant surgery were included in the study. Ten patients had received the allograft (Group 1) and ten had received autogenous connective tissue (Group 2).An independent examiner retrospectively analyzed the patient records at 7, 15, and 60 days, and five years post-procedure. Data from these observations were collected and analyzed using SPSS Statistics, version 25. Descriptive statistical analysis was conducted.

    RESULTS: All patients exhibited an increase in KT. For Group 1, the mean KT width measurements were 1.27 ± 0.46 mm at the initial evaluation, increasing to 2.00 ± 0.38 mm, 2.80 ± 0.78 mm, 3.27 ± 0.80 mm, and 3.01 ± 0.68 mm at 7, 15, and 60 days post-surgery (with prosthesis delivery on day 60), and five years after prosthetic rehabilitation, respectively.

    CONCLUSIONS: Within the limitations of this retrospective clinical study, both amnion/chorion and connective tissue show significant potential for KT expansion when used in conjunction with implant surgery.

    CLINICAL RELEVANCE: The use of allografts, due their low morbidity, and acceptable results should be considered as a viable option for soft tissues augmentations.

    MeSH terms: Adult; Dental Implantation, Endosseous/methods; Esthetics, Dental; Female; Humans; Keratins; Male; Middle Aged; Retrospective Studies; Treatment Outcome; Allografts
  16. Khan FF, Haq MSU, Ashfaq A, Saud M, Ibrahim A
    Cult Med Psychiatry, 2025 Jan 25.
    PMID: 39856384 DOI: 10.1007/s11013-024-09892-2
    The present study has explored the folk knowledge about the phenomenon of sleep paralysis in Pakistani society. The research aimed to gain a nuanced glimpse focusing on three major factors, culture, religion, and gender, that influence the lived experiences of those who face sleep paralysis. In this qualitative research, to have a holistic perception of indigenous knowledge about it, we selected both male and female participants who have experienced sleep paralysis. The findings indicate that there is an influence of Pakistani culture and religion regarding the experiences of the people with sleep paralysis, and gender is linked with the folklore on creatures that were linked to fairy tales and Islamic teachings. The study also revealed that Pakistani ethnic diversity has created a pool of versatility for identifying different experiences regarding sleep paralysis. These experiences were not just a part of the medical situation but portrayed the multicultural facets that are embedded in the individuals throughout their lives. Lastly, the study suggests that there is a complexity within the interactions between culture, religion, and gender on sleep paralysis. This needs to be further investigated to create culturally appropriate therapies that may have a favorable effect on both physical and mental health outcomes.
  17. Forbes EJ, Tiego J, Langmead J, Unruh KE, Mosconi MW, Finlay A, et al.
    J Autism Dev Disord, 2025 Jan 24.
    PMID: 39856431 DOI: 10.1007/s10803-024-06718-3
    Oculomotor characteristics, including accuracy, timing, and sensorimotor processing, are considered sensitive intermediate phenotypes for understanding the etiology of neurodevelopmental conditions, such as autism and ADHD. Oculomotor characteristics have predominantly been studied separately in autism and ADHD. Despite the high rates of co-occurrence between these conditions, only one study has investigated oculomotor processes among those with co-occurring autism + ADHD. Four hundred and five (n = 405; 226 males) Australian children and adolescents aged 4 to 18 years (M = 9.64 years; SD = 3.20 years) with ADHD (n = 64), autism (n = 66), autism + ADHD (n = 146), or neurotypical individuals (n = 129) were compared across four different oculomotor tasks: visually guided saccade, anti-saccade, sinusoidal pursuit and step-ramp pursuit. Confirmatory analyses were conducted using separate datasets acquired from the University of Nottingham UK (n = 17 autism, n = 22 ADHD, n = 32 autism + ADHD, n = 30 neurotypical) and University of Kansas USA (n = 29 autism, n = 41 neurotypical). Linear mixed effect models controlling for sex, age and family revealed that children and adolescents with autism + ADHD exhibited increased variability in the accuracy of the final saccadic eye position compared to neurotypical children and adolescents. Autistic children and adolescents demonstrated a greater number of catch-up saccades during step-ramp pursuit compared to neurotypical children and adolescents. These findings suggest that select differences in saccadic precision are unique to autistic individuals with co-occurring ADHD, indicating that measuring basic sensorimotor processes may be useful for parsing neurodevelopment and clinical heterogeneity in autism.
  18. Tiwari S, Khatib MN, Mm R, Kaur M, Sharma GC, Sudan P, et al.
    BMC Infect Dis, 2025 Jan 24;25(1):111.
    PMID: 39856559 DOI: 10.1186/s12879-025-10498-1
    BACKGROUND: Dengue and leptospirosis are prominent vector-borne diseases in tropical and subtropical regions, sharing overlapping geographic distribution and clinical presentations, which complicates diagnosis and management. Co-infection of these pathogens places additional strain on healthcare resources in endemic areas. This study aims to systematically estimate the prevalence and mortality rates of dengue and leptospirosis co-infections and assess their clinical implications.

    METHODS: Adhering the PRISMA 2020 guidelines and registered in the PROSPERO database, we conducted a systematic review and meta-analysis using the PubMed, Embase, and Web of Science databases up to October 2024. Nested Knowledge was used for screening and data extraction. Studies reporting quantitative data on the prevalence or mortality of dengue and leptospirosis co-infections were included. Data extraction and quality assessment were performed independently by two reviewers using the Modified Newcastle-Ottawa Scale. Statistical analyses, including prevalence and mortality estimation, sensitivity analysis were conducted using R, with heterogeneity evaluated by the I² statistic.

    RESULTS: Out of 3,982 records, 14 studies met the eligibility criteria, yielding a pooled prevalence of dengue and leptospirosis co-infection at 2.33% (95% CI: 1.41-3.46%) across 16,638 participants, with significant heterogeneity (I² = 90%). The prediction interval for co-infection ranged from 0.05 to 7.27%. The pooled mortality rate among co-infected patients was 9.96% (95% CI: 0-53.49%), with moderate heterogeneity (I² = 71%). The prediction interval for mortality ranged from 0.00 to 100%. Publication bias was indicated by an LFK index of 2.52.

    CONCLUSION: This meta-analysis revealed a moderate prevalence and a notable mortality rate for dengue and leptospirosis co-infections, with significant variability observed across different studies. Further research into the immunopathology and the implementation of integrated surveillance systems could enhance the effectiveness of diagnosis and treatment strategies in regions where these diseases are endemic.

    MeSH terms: Humans; Prevalence
  19. Idris IB, Dahlan SA, Rahman RA, Nawi AM
    BMC Public Health, 2025 Jan 24;25(1):317.
    PMID: 39856579 DOI: 10.1186/s12889-024-20784-3
    BACKGROUND: The prevalence of diabetes mellitus among women increased consistently together with the increase in the overall prevalence of diabetes mellitus globally. One of the components in holistic diabetes care among women are preconception interventions. Family planning usage has been one of the components in preconception care among this group of population, especially among women with unoptimised diabetes mellitus, where family planning may allow disease optimisation prior to pregnancy. This systematic review thus aimed to synthesise evidences and improve understanding on the non-individual factors in influencing family planning practice among women with diabetes mellitus.

    METHODS: PubMed, Web of Science and EBSCOHost was systematically searched for empirical studies between 2000 and 2023 that discussed on factors that influenced family planning usage among women with diabetes. This systematic literature review was conducted in accordance to Joanna Briggs Institute's approach for conducting systematic review of associations. Factors were categorised to either individual and non-individual factors. Narrative synthesis approach was adopted that appropriately accommodates the heterogeneity of the reviewed studies.

    RESULTS: A total of 29 studies met the inclusion criteria. Studies included in this review mostly reported individual-level factors that influence family planning practice among women with diabetes mellitus which were mainly the presence of diseases and other sociodemographic characteristics. Only six studies reported factors beyond individual variables which include geographic region, access to care, opinion of significant others, healthcare providers' perception, role of doctors and types of service providers.

    CONCLUSIONS: This systematic review provides evidences that highlighted the gap in knowledge on variables that were beyond individual-level factors which influence family planning practice among women with diabetes mellitus. Further studies that explored structural and systemic factors may benefit future program planning to identify and target modifiable factors.

    MeSH terms: Adult; Female; Humans; Pregnancy; Preconception Care
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