Browse publications by year: 2025

  1. Tan KF, Chia LY, Maki MAA, Cheah SC, In LLA, Kumar PV
    PMID: 39878813 DOI: 10.1007/s00210-025-03839-z
    The third most prevalent type of cancer in the world, colorectal cancer, poses a significant treatment challenge due to the nonspecific distribution, low efficacy, and high systemic toxicity associated with chemotherapy. To overcome these limitations, a targeted drug delivery system with a high cytotoxicity against cancer cells while maintaining a minimal systemic side effects represents a promising therapeutic approach. Therefore, the aim of this study was to develop an efficient gold nanocarrier for the targeted delivery of the anticancer agent everolimus to Caco-2 cells. A novel gold nanocomposite (EV-β-CD-HA-Chi-AuNCs) functionalized with a targeting ligand (hyaluronic acid), a permeation enhancement excipient (chitosan), and an anticancer inclusive compound consisting of beta-cyclodextrin and everolimus was proposed and prepared via Turkevich method. Characterization was performed with a UV spectrometer, FTIR, Zetasizer, and HRTEM. Its drug release profile was also evaluated in media with three different pH values. Cytotoxicity and biocompatibility studies were performed on a colorectal cancer cell line (Caco-2) and a normal fibroblast line (MRC-5), respectively, via xCELLigence real-time cellular analysis (RTCA) technology. The inhibitory effect on migration was also further tested via the xCELLigence RTCA technique and a scratch assay. Characterization studies revealed the successful formation of EV-β-CD-HA-Chi-AuNCs with a size and charge which are suitable for the use as targeted drug delivery carrier. In the cytotoxic study, the EV-β-CD-HA-Chi-AuNCs showed a lower IC50 (16 ± 1 µg/ml) than the pure drug (25 ± 3 µg/ml) toward a colorectal cell line (Caco-2). In the biocompatibility study, the EV-β-CD-HA-Chi-AuNCs have minimal toxicity, while the pure drug has severe toxicity toward healthy fibroblasts (MRC-5) despite its low concentration. In the cell migration study, the EV-β-CD-HA-Chi-AuNCs also showed a greater inhibitory effect than the pure drug. Compared with the pure drug, the EV-β-CD-HA-Chi-AuNCs exhibit an excellent selective cytotoxicity between cancerous colorectal Caco-2 cells and healthy MRC-5 cells, making it a potential carrier to carry the drug to the cancerous site while maintaining its low toxicity to the surrounding environment. In addition, an increase in the cytotoxic activity of the EV-β-CD-HA-Chi-AuNCs toward cancerous colorectal Caco-2 cells was also observed, which can potentially improve the treatment of colorectal cancer.
  2. Al-Tameemi AI, Masarudin MJ, Rahim RA, Mizzi R, Timms VJ, Isa NM, et al.
    Appl Microbiol Biotechnol, 2025 Jan 29;109(1):32.
    PMID: 39878901 DOI: 10.1007/s00253-024-13355-4
    The rapid advancement of nanotechnology, particularly in the realm of pharmaceutical sciences, has significantly transformed the potential for treating life-threatening diseases. A pivotal aspect of this evolution is the emergence of "green nanotechnology," which emphasizes the environmentally sustainable synthesis of raw materials through biological processes. This review focuses on the biological synthesis and application of zinc oxide (ZnO) nanoparticles (NPs) from probiotic bacteria, particularly those sourced from wastewater. Microorganisms from wastewater tolerate harmful elements and enzymatically convert toxic heavy metals into eco-friendly materials. These probiotic bacteria are instrumental in the synthesis of ZnO NPs and exhibit remarkable antimicrobial properties with diverse industrial applications. As the challenge of drug-resistant pathogens escalates, innovative strategies for combating microbial infections are essential. This review explores the intersection of nanotechnology, microbiology, and antibacterial resistance, highlighting the importance of selecting suitable probiotic bacteria for synthesizing ZnO NPs with potent antibacterial activity. Additionally, the review addresses the biofunctionalization of NPs and their applications in environmental remediation and therapeutic innovations, including wound healing, antibacterial, and anticancer treatments. Eco-friendly NP synthesis relies on the identification of these suitable microbial "nano-factories." Targeting probiotic bacteria from wastewater can uncover new microbial NP synthesis capabilities, advancing environmentally friendly NP production methods. KEY POINTS: • Innovative strategies are needed to combat drug-resistant pathogens like MRSA. • Wastewater-derived probiotic bacteria are an eco-friendly method for ZnO synthesis. • ZnO NPs show significant antimicrobial activity against various pathogens.
    MeSH terms: Probiotics*; Nanotechnology; Nanoparticles/chemistry; Metal Nanoparticles/chemistry; Green Chemistry Technology
  3. Balakrishnan V, Ahhmed U, Basheer F
    PLoS One, 2025;20(1):e0317232.
    PMID: 39879178 DOI: 10.1371/journal.pone.0317232
    BACKGROUND: Online malicious attempts such as scamming continue to proliferate across the globe, aided by the ubiquitous nature of technology that makes it increasingly easy to dupe individuals. This study aimed to identify the predictors for online fraud victimization focusing on Personal, Environment and Behavior (PEB).

    METHODS: Social Cognitive Theory (SCT) was used as a guide in developing the PEB framework. Specifically, three factors were identified-Self-awareness (Personal), Attitude (Personal and Environment) and Safe Practice (Behavior) as the potential predictors for online fraud victimization. A self-reporting questionnaire was developed based on the PEB framework and used to collect data targeting Malaysian adults. The study reports result from two separate datasets collected across two separate timelines. Study I involved data collection in January 2023 (n = 820) whereas Study II was conducted with a modified questionnaire from November 2023 -January 2024 (n = 629). Study I identified the online fraud victimization predictors through an Exploratory Factor Analysis (EFA) and a hierarchical binary logistic regression. The dataset from Study II was used to validate the online fraud victimization model derived from Study I by executing another round of hierarchical binary logistic regression.

    RESULTS: Results from both the samples show that most of the respondents are aware of digital privacy. EFA from Study I yielded a five-factor solution with a total variance of 60.6%, namely, Self-awareness, Safe Practice, Bank Trust, Overconfidence and Social Influence. Hierarchical binary logistic regression results from both the studies were found to be consistent. Specifically, Overconfidence (β = 0.374; OR = 1.453; 95% CI [1.119, 1.887]; p = 0.005) and Social Influence (β = 0.332; OR = 1.225; 95% CI [1.077, 1.512]; p = 0.006) were found to significantly predict online fraud victimization as well as gender (β = 0.364; OR = 1.440; 95% CI [1.008, 2.016]; p = 0.045) with females exhibiting higher risks to victimization.

    IMPLICATIONS: The emergence of Overconfidence and Social Influence as significant predictors can guide the development of targeted online fraud awareness campaigns and/or tools emphasizing critical thinking and skepticism. Policymakers can leverage this knowledge to implement regulations that reduce deceptive practices online, promote digital literacy programs, and mandate clearer consumer protections to mitigate the impact of social manipulation and overconfidence on fraud victimization.

    CONCLUSION: This study identifies online fraud victimization predictors, hence improving our understanding of the factors behind this phenomenon-allowing for the development of effective preventive measures and policies to safeguard individuals and improve digital security. For instance, gender- specific educational campaigns can be developed to enhance awareness and equip women with strategies to detect and avoid scams. Additionally, addressing systemic factors like social norms and digital literacy gaps is crucial for creating equitable and effective solutions to reduce online fraud victimization.

    MeSH terms: Adolescent; Adult; Attitude; Female; Fraud; Humans; Malaysia; Male; Middle Aged; Surveys and Questionnaires; Internet; Young Adult; Self Report
  4. Mulenga M, Rajamanikam A, Kumar S, Muhammad SB, Bhassu S, Samudid C, et al.
    PLoS One, 2025;20(1):e0316493.
    PMID: 39879257 DOI: 10.1371/journal.pone.0316493
    The emergence of Next Generation Sequencing (NGS) technology has catalyzed a paradigm shift in clinical diagnostics and personalized medicine, enabling unprecedented access to high-throughput microbiome data. However, the inherent high dimensionality, noise, and variability of microbiome data present substantial obstacles to conventional statistical methods and machine learning techniques. Even the promising deep learning (DL) methods are not immune to these challenges. This paper introduces a novel feature engineering method that circumvents these limitations by amalgamating two feature sets derived from input data to generate a new dataset, which is then subjected to feature selection. This innovative approach markedly enhances the Area Under the Curve (AUC) performance of the Deep Neural Network (DNN) algorithm in colorectal cancer (CRC) detection using gut microbiome data, elevating it from 0.800 to 0.923. The proposed method constitutes a significant advancement in the field, providing a robust solution to the intricacies of microbiome data analysis and amplifying the potential of DL methods in disease detection.
    MeSH terms: Gastrointestinal Microbiome*; Algorithms; Humans; Neural Networks (Computer); Area Under Curve; High-Throughput Nucleotide Sequencing/methods; Microbiota
  5. Sutan R, Ismail S, Ibrahim R
    JMIR Hum Factors, 2025 Jan 29;12:e53630.
    PMID: 39879614 DOI: 10.2196/53630
    BACKGROUND: Evaluating digital health service delivery in primary health care requires a validated questionnaire to comprehensively assess users' ability to implement tasks customized to the program's needs.

    OBJECTIVE: This study aimed to develop, test the reliability of, and validate the Tele-Primary Care Oral Health Clinical Information System (TPC-OHCIS) questionnaire for evaluating the implementation of maternal and child digital health information systems.

    METHODS: A cross-sectional study was conducted in 2 phases. The first phase focused on content item development and was validated by a group of 10 experts using the content validity index. The second phase was to assess its psychometric testing for reliability and validity.

    RESULTS: A structured questionnaire of 65 items was constructed to assess the TPC-OHCIS delivery for primary health care use based on literature and has been validated by 10 experts, and 319 respondents answered the 65-item TPC-OHCIS questionnaire, with mean item scores ranging from 1.99 (SD 0.67) to 2.85 (SD 1.019). The content validity, reliability, and face validity showed a scale-level content validity index of 0.90, scale-level content validation ratio of 0.90, and item-level face validity index of 0.76, respectively. The internal reliability was calculated as a Cronbach α value of 0.90, with an intraclass correlation coefficient of 0.91. Scales were determined by the scree plot with eigenvalues >1, and 13 subscales were identified based on principal component analysis. The Kaiser-Meyer-Olkin value was 0.90 (P0.7. The Bartlett test of sphericity, determining construct validity, was found to be significant (P

    MeSH terms: Adult; Cross-Sectional Studies; Female; Humans; Male; Middle Aged; Oral Health; Primary Health Care*; Surveys and Questionnaires; Reproducibility of Results
  6. Sinnathamby A, Ong YT, Lim SX, Hiew AWH, Ng SY, Chee JH, et al.
    PMID: 39879618 DOI: 10.1177/10499091251317725
    BACKGROUND: Palliative Care, Geriatrics and Emergency physicians are exposed to death, terminally ill patients and distress of patients and their families. As physicians bear witness to patients' suffering, they are vulnerable to the costs of caring-the emotional distress associated with providing compassionate and empathetic care to patients. If left unattended, this may culminate in burnout and compromise professional identity. This study aims to provide a better understanding of suffering across various practice settings and specialties to guide the design of support frameworks for physicians and their patients.

    METHODS: From August 2023 to September 2024, semi-structured interviews were conducted with sixteen Palliative Care, 12 Geriatrics and 13 Emergency physicians from various hospitals in Malaysia. Interview transcripts were analyzed using both inductive and deductive qualitative analyses.

    RESULTS: Data analysis revealed three key domains: (1) living and dying well, (2) definition of suffering, and (3) impact of patient suffering on physicians.

    CONCLUSION: Physicians' concepts of a good life and death frame their notions of suffering beyond the antithesis of a good life. Suffering is found to be distress at a loss of control, independence and dignity, alongside the presence of physical, emotional and existential distress. Witnessing patient suffering predisposes to physician suffering as they question their goals and roles in patient care. Our findings underscore the need for host organizations, hospitals and clinical departments to invest more in the care of their physicians. We believe these findings ought to be applicable to many resource-limited nations and other health care professionals beyond Malaysian shores.

  7. Teh JL, Walvekar R, Ho KC, Khalid M
    J Environ Manage, 2025 Feb;375:124267.
    PMID: 39879924 DOI: 10.1016/j.jenvman.2025.124267
    Effective management of agricultural and industrial by-products is essential for promoting circular economic practices and enhancing environmental sustainability. Agri-food wastes and waste cooking oil (WCO) represent two abundant residual streams with significant potential for sustainable biolubricant production. Valorizing biomass and WCO aligns with Sustainable Development Goal (SDG) 7, as it improves energy efficiency through enhanced lubricant performance and reduced energy loss. Furthermore, this sustainable approach contributes to SDG 12 and SDG 13 by minimizing waste production and accumulation, thereby mitigating negative environmental impacts and climate change. This critical review addresses existing gaps in the production of biolubricants from WCO and the incorporation of natural antioxidants as versatile additives. It examines and compares various techniques for the extraction, chemical and physical modification, and characterization of WCO-derived biolubricants. Specific methods, including esterification, transesterification, and antioxidant incorporation, are evaluated for their effectiveness in converting WCO into biolubricants. The review also discusses the influence of residual bioactive compounds on oxidative stability and lubricating properties. While vegetable oils demonstrate superior friction-reducing capabilities compared to petroleum-based lubricants, their triglyceride structure often results in poor oxidative stability, limiting their practical applications. Modification strategies and antioxidant inclusion are proposed to enhance this stability. A comprehensive analysis of the physicochemical properties and tribological performance of biolubricants, both pre- and post-processing, is presented. This systematic evaluation of extraction and upgrading methodologies aims to facilitate the development and industrial adoption of sustainable biolubricants.
    MeSH terms: Cooking; Plant Oils/chemistry; Lubricants/chemistry
  8. Cortese S, Bellato A, Gabellone A, Marzulli L, Matera E, Parlatini V, et al.
    Cell Rep Med, 2025 Feb 18;6(2):101916.
    PMID: 39879991 DOI: 10.1016/j.xcrm.2024.101916
    The diagnosis of autism is currently based on the developmental history, direct observation of behavior, and reported symptoms, supplemented by rating scales/interviews/structured observational evaluations-which is influenced by the clinician's knowledge and experience-with no established diagnostic biomarkers. A growing body of research has been conducted over the past decades to improve diagnostic accuracy. Here, we provide an overview of the current diagnostic assessment process as well as of recent and ongoing developments to support diagnosis in terms of genetic evaluation, telemedicine, digital technologies, use of machine learning/artificial intelligence, and research on candidate diagnostic biomarkers. Genetic testing can meaningfully contribute to the assessment process, but caution is required when interpreting negative results, and more work is needed to strengthen the transferability of genetic information into clinical practice. Digital diagnostic and machine-learning-based analyses are emerging as promising approaches, but larger and more robust studies are needed. To date, there are no available diagnostic biomarkers. Moving forward, international collaborations may help develop multimodal datasets to identify biomarkers, ensure reproducibility, and support clinical translation.
    MeSH terms: Machine Learning*; Artificial Intelligence; Genetic Testing/methods; Humans; Biomarkers*; Telemedicine
  9. Razali MF, Amir Shah Ruddin NI, Abdul Rani NA, Mohamed F, Ahmad NI, Leong YH
    BMJ Open, 2025 Jan 28;15(1):e092681.
    PMID: 39880440 DOI: 10.1136/bmjopen-2024-092681
    OBJECTIVE: Glyphosate is widely used in Malaysian agriculture but poses a significant under-reported public health concern due to poisoning. This paper aims to study the epidemiology of glyphosate poisoning in Malaysia, assessing severity, identifying risk factors, and high-risk groups.

    SETTING: All glyphosate-related data of the Malaysia National Poison Centre from 2006 to 2023.

    PARTICIPANTS: Telephone inquiries of incidents involving glyphosate made by healthcare professionals across Malaysia. Information received was recorded according to WHO guidelines.

    OUTCOMES: The outcome is to provide an overview of national glyphosate poisoning epidemiology, including identifying risk factors and high-risk groups to strategise appropriate measures.

    RESULTS: A total of 4548 glyphosate poisoning cases were reported, with males comprising 67.3% of cases. The most affected age groups were 21-30 years (25.5%) and 31-40 years (23.5%). Ingestion was the primary exposure route (93.0%). Intentional poisonings, mainly suicides, accounted for 68.4% (n=3078) of cases. Among the 1420 unintentional cases, 78.2% resulted from ingestion exposures. The incidence rate declined from 1.17 per 100 000 in 2011 to 0.61 in 2023. Significant associations were found between intentional ingestion and sociodemographic factors. From 2013 to 2023, 73.7% of cases were minor, 11.5% moderate, 0.8% severe and 0.1% fatal.

    CONCLUSIONS: In Malaysia, the reported intentional glyphosate poisoning most commonly affects young adults, resulting in generally minor to moderate symptoms. The study highlights the need for stricter regulations on glyphosate use and improved mental health support, to mitigate poisoning risks. Continued surveillance and public education are essential to address this public health issue.

    MeSH terms: Adolescent; Adult; Aged; Child; Child, Preschool; Female; Humans; Infant; Malaysia/epidemiology; Male; Middle Aged; Poison Control Centers/statistics & numerical data; Poisoning/epidemiology; Risk Factors; Suicide/statistics & numerical data; Incidence; Age Distribution; Sex Distribution; Young Adult
  10. Wang H, Gao C, Guignard-Duff M, Cole C, Hall C, Baruah R, et al.
    Heart, 2025 Jan 29.
    PMID: 39880470 DOI: 10.1136/heartjnl-2024-324160
    BACKGROUND: Early heart failure (HF) diagnosis is crucial to ensure that optimal guideline-directed medical therapy (GDMT) is administered to reduce morbidity and mortality. Limited access to echocardiography could lead to a later diagnosis for patients, for example, during an HF hospitalisation (hHF). This study aimed to compare the incidence and outcomes of inpatient versus outpatient diagnosis of HF.

    METHODS: Electronic health records were linked to echocardiography data between 2015 and 2021 from patients in Tayside, Scotland (population~450 000). Incident HF diagnosis was classified into inpatient or outpatient and stratified by ejection fraction (EF). A non-HF comparator group with normal left ventricular function was also defined. The primary outcome was time to cardiovascular death or hHF within 12 months of diagnosis.

    RESULTS: In total, 5223 individuals were identified, 4231 with HF (1115 heart failure with reduced ejection fraction (HFrEF), 666 heart failure with mildly reduced ejection fraction, 1402 heart failure with preserved ejection fraction and 1048 HF with unknown EF) and 992 with non-HF comparators. Of the 4231 HF patients, 2169 (51.3%) were diagnosed as inpatients. The primary outcome was observed in 1193 individuals with HF (28.1%) and 32 (3.2%) non-HF comparators and was significantly more likely to occur in individuals diagnosed as inpatients than outpatients (809 vs 384 events; adjusted HR: 1.62 (1.39-1.89), p<0.001), and this was consistent regardless of EF. For HFrEF patients first diagnosed as inpatients, those discharged on ≥2 GDMT had a reduced incidence of the primary outcome compared with those discharged on <2 GDMT (303 vs 175 events; adjusted HR: 0.72 (0.55-0.94), p=0.016).

    CONCLUSIONS: Individuals whose first presentation was a HF hospitalisation had a significantly worse outcome than those who were diagnosed in the community. Among hospitalised individuals, higher use of GDMT was associated with improved outcomes. Our results highlight the importance of improving diagnostic pathways to allow for earlier identification and treatment of HF.

  11. Malvi A, Khatib MN, Balaraman AK, Roopashree R, Kaur M, Srivastava M, et al.
    BMC Pulm Med, 2025 Jan 29;25(1):48.
    PMID: 39881272 DOI: 10.1186/s12890-025-03516-0
    BACKGROUND: Cannabis is the third most widely used psychoactive substance globally, and its consumption has been increasing, particularly with the growing trend of legalization for medicinal and recreational use. Recent studies have raised concerns about the potential impact of cannabis on respiratory health, specifically the risk of asthma, a significant public health concern. This systematic review aimed to consolidate research on the association between cannabis use and the risk of asthma.

    METHODS: A comprehensive search was conducted across PubMed, Embase, and Web of Science, covering studies published up to September 30, 2024. We included peer-reviewed observational studies evaluating the link between cannabis consumption and the risk of asthma diagnosis. Data synthesis employed a random-effects meta-analysis to account for heterogeneity. R statistical software (version 4.4) was used for statistical analyses.

    RESULTS: The search yielded 8 relevant studies after screening 1,887 records. The pooled odds ratio (OR) for the association between cannabis consumption and the risk of asthma diagnosis was 1.31, 95% confidence interval (CI): 1.19-1.44, indicating greater odds of having asthma compared to non-users. Moderate heterogeneity was observed (I² = 46%), and sensitivity analysis confirmed the robustness of the findings.

    CONCLUSION: This systematic review and meta-analysis identifies a significant association between cannabis use and greater odds of having asthma. These findings emphasize the importance of raising awareness about the potential respiratory risks associated with cannabis use. Future research should prioritize identifying moderating factors, such as the frequency and mode of cannabis consumption, to enhance understanding of this association and provide a stronger evidence base for potential public health interventions.

    CLINICAL TRIAL NUMBER: Not applicable.

    MeSH terms: Cannabis/adverse effects; Humans; Marijuana Smoking/adverse effects; Marijuana Smoking/epidemiology; Risk Factors; Odds Ratio
  12. Shabil M, Padhi BK, Khatib MN, Menon SV, Kaur M, Kumari M, et al.
    J Cardiothorac Surg, 2025 Jan 29;20(1):107.
    PMID: 39881328 DOI: 10.1186/s13019-024-03161-4
    BACKGROUND: Proton pump inhibitors (PPIs) are commonly used for managing gastroesophageal disorders but concerns about their potential association with increased stroke risk have emerged, especially among patients with pre-existing cardiovascular conditions such as acute coronary syndrome (ACS). This systematic review and meta-analysis aim to assess the risk of stroke associated with PPI use, stratified by the presence or absence of pre-existing CVD.

    METHODS: This review was conducted following the PRISMA guidelines and included studies up to March 2024 from PubMed, Embase, and Web of Science. Eligible studies were longitudinal, including prospective cohorts, nested case-controls, and post-hoc analyses of RCTs that reported stroke outcomes in relation to PPI use. Data were synthesized using random-effects meta-analysis models in R software version 4.3.

    RESULTS: Our search yielded 41 studies encompassing over 800,000 participants globally. Meta-analysis of 14 observational studies revealed a slight but non-significant increased stroke risk among patients with prior CVD (pooled HR = 1.222, 95% CI: 0.963 to 1.481, I² = 78%). In contrast, analysis of 15 studies without prior CVD showed a modestly increased risk (pooled HR = 1.15, 95% CI: 1.023 to 1.288, I² = 98%). Five RCTs involving patients with CVD reported a pooled RR of 1.158 (95% CI: 0.914 to 1.466), indicating no significant risk increase.

    CONCLUSION: The association between PPI use and stroke risk appears modest and is influenced by the presence of cardiovascular conditions. Clinical decision-making should consider individual patient risk profiles, and further high-quality studies are needed to guide safer PPI prescribing practices.

    MeSH terms: Cardiovascular Diseases*; Humans; Risk Factors; Risk Assessment/methods; Stroke*
  13. Arman NZ, Aris A, Salmiati S, Rosli AS, Foze MF, Talib J
    Environ Sci Pollut Res Int, 2025 Jan;32(4):1766-1782.
    PMID: 39745626 DOI: 10.1007/s11356-024-35692-7
    In the Johor River Basin, a comprehensive analysis was conducted on 24 water environmental parameters across 33 sampling sites over 3 years, encompassing both dry and wet seasons. A total of 396 water samples were collected and analyzed to calculate the Water Quality Index (WQI). To further assess water quality and pinpoint potential pollution sources, multivariate techniques such as principal component analysis (PCA) and cluster analysis (CA), alongside spatial analysis using inverse distance weighted (IDW) interpolation, were employed. According to the National Water Quality Standard, most of the analyzed physicochemical components fall within Classes II and III, albeit with varying concentrations. However, certain sites exhibited levels of BOD5, TSS, and nutrients such as total nitrogen (TN) and total phosphorus (TP) that exceeded the threshold level of water quality standards, signaling pollution from diverse sources. Notably, all trace elements, with the exception of copper (Cu) and nickel (Ni), remained within the acceptable limits set by WHO guidelines and the National Water Quality Standard. PCA revealed parameter groupings linked to factors such as soil erosion, salinity, wastewater discharge, and fecal contamination, which are key determinants of water quality. The cluster analysis categorized the 33 sampling sites into three distinct clusters, each reflecting the geological setting and varying levels of pollution. The IDW-based spatial distribution indicated significant water quality degradation as the river flows downstream, particularly in regions experiencing rapid agricultural, industrial, and residential development. These activities contribute to the breakdown of organic matter and the release or overflow of wastewater into nearby river systems. This study highlights the effectiveness of integrating data-driven methodologies for surface water quality assessment, offering valuable insights for sustainable watershed management.
    MeSH terms: Malaysia; Nitrogen/analysis; Phosphorus/analysis; Multivariate Analysis; Principal Component Analysis; Water Quality*
  14. Fakhri Y, Mahmoudizeh A, Hemmati F, Adiban M, Esfandiari Z, Mousavi Khaneghah A
    PMID: 39871486 DOI: 10.1080/09603123.2025.2453971
    Malachite Green (MG) is an antibiotic with antifungal activity, which is illegal to use in agriculture due to its mutagenic and teratogenic properties. Several scientific papers have been published on MG in fish. Therefore, an attempt was made to determine the meta-analysis concentration of MG in fish based on countries and types of fish subgroups, as well as the health risks of consumers, using the Monte Carlo simulation (MCS) model. The three countries with the highest concentration of MG were Iran (14.697 µg/kg), Brazil (2.840 µg/kg), and China (2.277 µg/kg). The overall pooled concentration of MG in fish was 3.036 µg/kg, 95%CI (2.860-3.212 µg/kg), and the highest concentration of MG was observed in Pacu fish (6.603 µg/kg). The health risk assessment shows adults in Malaysia, China, and Iran and children in Italy, Spain, Brazil, Malaysia, China, and Iran are at considerable risk (MOE <10,000); hence, carrying out control plans in these countries is recommended.
  15. Schandl S, Osondu-Chuka G, Guagliano G, Perak S, Petrini P, Briatico-Vangosa F, et al.
    J Mater Chem B, 2025 Feb 19;13(8):2796-2809.
    PMID: 39871625 DOI: 10.1039/d4tb02675f
    The reason why certain bacteria, e.g., Pseudomonas aeruginosa (PA), produce acetylated alginate (Alg) in their biofilms remains one of the most intriguing facts in microbiology. Being the main structural component of the secreted biofilm, like the one formed in the lungs of cystic fibrosis (CF) patients, Alg plays a crucial role in protecting the bacteria from environmental stress and potential threats. Nonetheless, to investigate the PA biofilm environment and its lack of susceptibility to antibiotic treatment, the currently developed in vitro biofilm models use native seaweed Alg, which is a non-acetylated Alg. The role of the acetyl side group on the backbone of bacterial Alg has never been elucidated, and the transposition of experimental results obtained from such systems to clinical conditions (e.g., to treat CF-infection) may be hazardous. We systematically investigated the influence of acetylation on the physico-chemical and mechanical properties of Alg in solution and Ca2+-crosslinked hydrogels. Furthermore, we assessed how the acetylation influenced the interaction of Alg with tobramycin, a common aminoglycoside antibiotic for PA. Our study revealed that the degree of acetylation directly impacts the viscosity and Young's Modulus of Alg in a pH-dependent manner. Acetylation increased the mesh size in biofilm-like Alg hydrogels, directly influencing antibiotic penetration. Our results provide essential insights to create more clinically relevant in vitro infection models to test the efficacy of new drugs or to better understand the 3D microenvironment of PA biofilms.
    MeSH terms: Acetylation; Anti-Bacterial Agents/pharmacology; Anti-Bacterial Agents/chemistry; Tobramycin/pharmacology; Tobramycin/chemistry; Hydrogels/pharmacology; Hydrogels/chemistry
  16. Lora Mantilla AJ, Parra Gomez LA, Camacho-López PA, Otero-Wandurraga J, Novella B, González-Medina A, et al.
    Heliyon, 2025 Jan 30;11(2):e41726.
    PMID: 39872457 DOI: 10.1016/j.heliyon.2025.e41726
    INTRODUCTION: Cardiovascular diseases are the leading cause of death and morbidity worldwide, with a significantly higher burden in low- and middle-income countries. Hypertension, a major risk factor for cardiovascular morbidity and mortality, remains under-diagnosed and poorly controlled, especially in regions such as Latin America. The HOPE-4 study demonstrated that the involvement of non-physician health workers (NPHWs), the use of standardized treatment algorithms, the provision of free antihypertensive drugs and home follow-up can significantly improve hypertension control and reduce cardiovascular risk, as demonstrated in Colombia and Malaysia. On this basis, the COTRACO study aims to address the barriers to hypertension treatment in low- and middle-income countries by implementing a similar standardized treatment approach delivered by non-specialist health workers.

    METHODOLOGY: The COTRACO study is a quasi-experimental, parallel-group, non-randomized, before-and-after study. A community-based model will be implemented in 600 patients in Colombia and the Dominican Republic, involving NPHWs to: 1) apply standardized treatment algorithms, 2) promote adherence to healthy lifestyles, and 3) provide standardized pharmacological treatment. Propensity Score Matching will be used to select 300 patients in Chile and 1200 in Spain for comparison with standard care in these populations.

    EXPECTED OUTCOMES: The primary outcome at 12 months of follow-up is the percentage of patients achieving controlled hypertension (defined as systolic BP 

  17. Muryani A, Aripin D, Dharsono HDA, Rajion ZA, Wicaksono S
    PMID: 39872903 DOI: 10.2147/CCIDE.S491632
    PURPOSE: Guided access cavity preparation (GACP) is an endodontic procedure utilizing stents, guide sleeves, or dynamic guides to facilitate the proper formation of access cavities. This paper aims to evaluate the significance of research on guided access cavity preparation in endodontic treatment concerning dentin preservation. In the context of dentin preservation, this paper provides a thorough scoping review of a variety of methodologies for evaluating the accuracy of guided access cavity preparation.

    MATERIALS AND METHODS: Article searches were conducted using Tthe keywords "Guide Access Cavity Preparation" AND "Static Guide OR 3D Printing Template" AND "Dynamic Guide through digital databases including PubMed, Scopus, Cochrane Library, Science Direct, and Google Scholar. Research articles published in English within the past five years (2019-2024) and in accordance with the PCC (Population, Concept, and Context) framework were also included in the study. Articles from the meta-analysis or systematic review study type, those that were not accessible in full text or in a paid format, and those that did not assess the use of guided endodontics in endodontic surgery were excluded. This scoping review adheres to the Preferred Reporting Items for Systematic Reviews without Meta-Analyses extension for Scoping Reviews (PRISMA-ScR) criteria.

    RESULTS: A total of 12 articles were reviewed, encompassing various locations, designs, type, and samples, which demonstrated the use of guided access cavity preparation has significant clinical applications and can provide accurate results in endodontic therapy.

    CONCLUSION: Article searches were conducted using the keywords "Guide Access Cavity Preparation" AND 'Static Guide OR 3D Printing Template' AND" Dynamic Guide through digital databases including PubMed, Scopus, Cochrane Library, Science Direct, and Google Scholar. Research articles published in English within the past five years (2019-2024) and in accordance with the PCC (Population, Concept, and Context) framework were also included in the study. Articles from the meta-analysis or systematic review study type, those that were not accessible in full text or in a paid format, and those that did not assess the use of guided endodontics in endodontic surgery were excluded. This scoping review adheres to the Preferred Reporting Items for Systematic Reviews without Meta-Analyses extension for Scoping Reviews (PRISMA-ScR) criteria. Studies show that advanced technologies in guided access cavity preparation endodontic treatment can improve dentin preservation, improve accuracy and predictability, particularly for root canal anomalies and difficult teeth, but clinicians must consider limitations and clinical applications. Clinicians must evaluate the limitations and clinical applications of guided endodontic access prior to its implementation.

  18. Lai NM, Fiander M, Cracknell J, Tan K, Romantsik O
    Cochrane Database Syst Rev, 2025 Jan 28;1(1):CD014212.
    PMID: 39873291 DOI: 10.1002/14651858.CD014212
    This is a protocol for a Cochrane Review (intervention). The objectives are as follows: To assess the benefits and harms of dexmedetomidine compared with opioids, non-opioids and placebo in providing sedation and analgesia for procedural pain in newborn infants.
    MeSH terms: Analgesia/methods; Humans; Hypnotics and Sedatives/administration & dosage; Hypnotics and Sedatives/adverse effects; Hypnotics and Sedatives/therapeutic use; Infant, Newborn; Randomized Controlled Trials as Topic*
  19. Reddy G, Gleibs IH
    Br J Soc Psychol, 2025 Apr;64(2):e12848.
    PMID: 39873313 DOI: 10.1111/bjso.12848
    This paper sheds light on how spaces become contested sites for identity construction and negotiation to take place. Applying the Social Representations Approach, a qualitative study of 10 focus group discussions (n = 39), was conducted in Singapore, Malaysia and the UK to explore how, and why racialised identity construction changed in each socio-political context. The study challenged two underlying assumptions in social psychology: (1) that the meaning of the racialised category holds constant across time and space, and (2) there exists a pan-racial identification among Asian identities, for example, which at times allows for racialised categories to be manipulated as variables. We argue that the distinction between the country that the racialised identity originates from, country of birth (or citizenship) for the individual and country that the individual manages the identity in, is important in understanding the changes in the psychology of racialised identities. By taking into consideration the interplay of temporality, space, social relations and social systems, this paper presents a contribution in the form of the concept "politicized psycho-geographies".
    MeSH terms: Adult; Female; Great Britain; Humans; Malaysia/ethnology; Male; Politics*; Singapore; Social Identification*; Focus Groups; Qualitative Research
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