Browse publications by year: 2025

  1. Bhuiyan MSA, Gupta SD, Silip JJ, Talukder S, Haque MH, Forwood JK, et al.
    Virology, 2025 Mar;604:110399.
    PMID: 39884161 DOI: 10.1016/j.virol.2025.110399
    Infectious bronchitis virus (IBV), an avian coronavirus, member of the genus Gammacoronavirus, poses significant threats to poultry health, causing severe respiratory, reproductive, and renal infections. The genetic diversity of IBV, driven by mutations, recombination and deletions, has led to the emergence of numerous serotypes and genotypes, complicating both diagnosis and control measures. Rapid and accurate diagnostic tools are essential for effective disease management and minimizing economic losses. Conventional diagnostic methods, such as PCR, virus isolation, and serological assays, are hindered by limitations in sensitivity, specificity, and turnaround time. In contrast, innovative biosensor platforms employing advanced detection mechanisms-including electrochemical, optical, and piezoelectric sensors-offer a transformative solution. These technologies provide portable, highly sensitive, and rapid diagnostic platforms for IBV detection. Beyond addressing the challenges of conventional methods, these biosensor-based approaches facilitate real-time monitoring and enhance disease surveillance. This review highlights the transformative potential of biosensors and their integration into diagnostic strategies for avian coronavirus infections, presenting them as a promising alternative for precise and efficient IBV detection.
    MeSH terms: Animals; Birds/virology; Poultry/virology; Sensitivity and Specificity
  2. Kannan VV, Kanabar B, Gowrishankar J, Khatibi A, Kamangar S, Arabi AIA, et al.
    Sci Rep, 2025 Jan 30;15(1):3833.
    PMID: 39885203 DOI: 10.1038/s41598-025-87640-1
    Meta-heuristic optimization algorithms are widely applied across various fields due to their intelligent behavior and fast convergence, but their use in optimizing engine behavior remains limited. This study addresses this gap by integrating the Design of Experiments-based Response Surface Methodology (RSM) with meta-heuristic optimization techniques to enhance engine performance and emissions characteristics using Tectona Grandi's biodiesel with Elaeocarpus Ganitrus as an additive. Advanced Machine Learning (ML) models, including Artificial Neural Networks (ANN), K-Nearest Neighbors (KNN), Extreme Gradient Boosting (XGB), and Random Trees (RT), were employed for predictive analysis, with ANN outperforming RSM in accuracy. The study identified the Teak biodiesel blend (TB20) with a 5 ml Elaeocarpus Ganitrus additive (TB20 + R5) as the optimal formulation, achieving the highest Brake Thermal Efficiency and reduced Brake-Specific Fuel Consumption. Desirability analysis further confirmed the blend's superior performance and emissions characteristics, with a desirability rating of 0.9282. This work highlights the potential of hybrid optimization approaches for improving biodiesel performance and emissions without engine modifications, contributing to the advancement of sustainable energy practices in internal combustion engines.
  3. Muttiah B, Law JX
    NPJ Sci Food, 2025 Jan 30;9(1):12.
    PMID: 39885215 DOI: 10.1038/s41538-025-00375-1
    Milk is a nutrient-rich liquid produced by mammals, offering various health benefits due to its composition of proteins, fats, carbohydrates, vitamins, and minerals. Beyond traditional nutritional aspects, recent research has focused on extracellular vesicles (EVs) found in milk and their potential health benefits, especially for gastrointestinal (GI) health. Milk-derived EVs have been shown to influence gut microbiota, promote gut barrier integrity, support tissue repair and regeneration, modulate immune responses, and potentially aid in managing conditions like inflammatory bowel disease (IBD) and colorectal cancer. This review discusses the current understanding of milk-EVs' effects on gut health, highlighting their potential therapeutic applications and future research directions. These findings underscore the promising role of milk-derived EVs in advancing GI health and therapeutics, paving the way for innovative approaches in oral drug delivery and targeted treatments for GI disorders.
  4. Salari N, Rahimi A, Zarei H, Abdolmaleki A, Rasoulpoor S, Shohaimi S, et al.
    BMC Pregnancy Childbirth, 2025 Jan 30;25(1):90.
    PMID: 39885489 DOI: 10.1186/s12884-025-07182-2
    BACKGROUND: Toxoplasmosis in pregnancy is associated with serious and irreversible maternal and fetal detrimental consequences. Also, different seroprevalence of Toxoplasma gondii in pregnancy is reported in many countries. The present systematic review and meta-analysis study aimed to determine the global seroprevalence of Toxoplasma gondii in pregnant women.

    METHODS: This study was conducted based on the PRISMA 2020 criteria. Initial searching was conducted using MeSH (Medical Subject Headings)-based keywords with no time limitation (by August 1, 2024). Collected papers were transferred to Citation Management Software (EndNote). Duplicate studies were merged and primary and secondary screenings were applied based on the inclusion/exclusion criteria. Validation was considered to find high-quality assessments. Finally, eligible extractable papers were enrolled for data collection. Data was analyzed using Comprehensive Meta-Analysis software (v.2) The random effects model was used in case of I2 index above 50%.In order to investigate the factors affecting the heterogeneity of studies, meta-regression tests were used to examine factors such as sample size and year of study.

    RESULTS: One hundred thirty-eight eligible studies with a total sample size of 135,098 pregnant women individuals were selected for data extraction and analysis. The heterogeneity index was found high (I2:98.9) and the random effect model was used for analysis. The egger test revealed the absence of publication bias in collected studies (p:0.088). Thus, the global seroprevalence of Toxoplasma gondii in pregnant women was reported at 36.6% (95%CI:33.7-39.6). the highest prevalence reported based on meta-analysis was reported in South America with 52.8% (95% CI:46.6-59), while only 15 studies were reviewed in this continent, most of which were in Brazil. Therefore, after the continent, the highest prevalence reported was reported in Africa with 46.8% (95% CI:39.5-54.3). Also, the lowest prevalence reported based on meta-analysis was in North America with 19.7% (95% CI:8.4-39.6) and Europe with 24.6% (95% CI:17.8-32.9).

    CONCLUSION: This study revealed a high level of seroprevalence of Toxoplasma gondii in pregnant women worldwide. This value mostly depends on the individual's age, lifestyle, and disease awareness regarding toxoplasmosis in pregnant women. Thus, public awareness, along with comprehensive health programs regarding the detrimental effects of toxoplasmosis in pregnant women, seems necessary for prevention or even early diagnosis of toxoplasmosis in pregnant women.

    MeSH terms: Female; Humans; Pregnancy; Global Health/statistics & numerical data; Pregnancy Complications, Parasitic/epidemiology; Seroepidemiologic Studies
  5. Liu S, Du Z, Song L, Liu H, Tee CATH, Liu H, et al.
    J Orthop Surg Res, 2025 Jan 30;20(1):112.
    PMID: 39885604 DOI: 10.1186/s13018-025-05484-x
    BACKGROUND: Knee Osteoarthritis (KOA) is a prevalent condition worldwide, significantly diminishing quality of life and productivity. Except for the alignment change, muscle activation patterns (MAP) have garnered increasing attention as another crucial factor contributing to KOA.

    OBJECTIVE: This study explores the factors, characteristics, and effects of MAP changes caused by KOA, providing a neuromuscular-based causal analysis for the rehabilitation treatment of KOA.

    METHODS: Keywords including the association of MAP with KOA will be included. "Knee, Osteoarthritis, Electromyography(EMG), Muscle Activity patterns, activation amplitudes, activation time, Muscle Synergy, Co-contraction/activation" were used to search the databases of Science Direct, PubMed, Scopus, and Wiley. The criteria include studies from the past fifteen years that document changes in muscle contraction characteristics and causality analysis in patients with KOA. we compared MAP changes between individuals with and without KOA, such as the activation amplitudes, activation time, muscle synergy and co-contraction index(CCI). Additionally, we explored the potential relationship between muscle weakness, pain, and lower limb mechanical changes with the variations of MAP.

    RESULTS: A total of 832 articles were reviewed, and 44 articles that met the inclusion criteria were selected for analysis. The changes in biomechanical structure, pain, and muscle atrophy may contribute to the formation and progression of the changes in MAP in KOA patients. In moderate KOA patients, the vastus lateralis (VL) and biceps femoris (BF) exhibits larger activation amplitudes, with earlier and longer activation times. The vastus medialis (VM) shows a delayed activation time relative to VL. Gastrocnemius activation time is prolonged during mid-gait, while the soleus exhibits lower activation amplitudes during the late stance phase. There are fewer, merged synergies with prolonged activation coefficients, and a higher percentage of unclassifiable synergies. Additionally, the CCI is positively correlated with task difficulty and symptoms. It is higher in the medial and lateral than hamstrings and quadriceps, and CCI specifically respond to joint stabilisation and load.

    CONCLUSION: In patients with moderate KOA, changes in MAP are mainly related to symptoms and the difficulty of tasks. MAP changes primarily result in variations in amplitude, contraction duration, muscle synergy, and CCI. The MAP changes can subsequently affect the intermuscular structure, pain, joint loading, and stiffness.

    CLINICAL IMPLICATIONS: These contribute to the progression of KOA and create a vicious cycle that accelerates disease advancement. Clinical rehabilitation treatments can target the MAP changes to break the cycle and help mitigate disease progression.

    MeSH terms: Biomechanical Phenomena/physiology; Electromyography*; Humans; Male
  6. Saadu Itas Y, Khandaker MU, Mahmoud M
    RSC Adv, 2025 Jan 29;15(5):3047-3059.
    PMID: 39885860 DOI: 10.1039/d4ra08592b
    Investigations on two-dimensional materials for efficient carbon dioxide (CO2) capture and storage have recently attracted much attention, especially in the global industrial sector. In this work, the CO2 uptake by three configurations of two-dimensional magnesium oxide was investigated using density functional theory. CO2 capture analysis was performed considering the geometrical, thermophysical, vibrational, electronic and optical properties. Results indicated that CO2 adsorption by magnesium oxide (MgO) sheets is a spontaneous process accompanied by a decrease in Gibbs free energy. Moreover, the CO2 molecular entropy and enthalpy of the CO2 adsorbed sheet were decreased, indicating that the entire process was enthalpy-driven. Among the pristine, vacant and nickel-doped (Ni-doped) MgO sheets, the Ni-doped system was found to have the highest values of Gibbs free energy, enthalpy and entropy in the order of -51.366 kJ mol-1-K, -65.105 kJ mol-1 and 127.606 J mol-1, respectively. It was also found to adsorb CO2 in the ultraviolet and visible (UV-Vis) regions within the range of 100-850 nm. Electronic interactions demonstrated that metallicity was significantly induced on the MgO sheet Ni impurity states, which enhanced the adsorption ability. Notably, hybrid orbitals between p y and p z revealed strong physisorption, as confirmed by the partial density of states (PDOS). The findings of this research promote CO2 capture sustainability by encouraging future experimentalists to use two-dimensional MgO as a better surface for CO2 capture.
  7. Sankar S, Balakrishnan P, Yong YK, Raju S, Velu V, Shankar EM, et al.
    Can J Infect Dis Med Microbiol, 2025;2025:6683501.
    PMID: 39885897 DOI: 10.1155/cjid/6683501
    The monkeypox (Mpox) virus has emerged as a global public health emergency of international concern recently. The virus that was endemic in West and Central Africa has now been reported with chains of global transmission to several countries. A scoping review was carried out from the relevant literature available from PubMed, Scopus and Web of Science. This comprehensive analysis describes the virus epidemiology, pathogenesis, clinical manifestations, complications including secondary bacterial infections, diagnosis, treatment and vaccination. The article underscores the significance of key viral and immune mediators of infection and discusses updated recommendations on therapeutic strategies and vaccination.
  8. Azim H, Johnston AL, Nixon M, Woodliffe JL, Theunissen R, Suresh R, et al.
    ACS Sustain Chem Eng, 2025 Jan 27;13(3):1164-1173.
    PMID: 39886474 DOI: 10.1021/acssuschemeng.4c10171
    We illustrate the importance of early career perspectives and diverse partnerships to develop solutions and overcome key challenges to achieve the Sustainable Development Goals.
  9. Thahe AA, Qaeed MA, Hilal N, Abubakar D, Bidin N, Allam NK
    Nanoscale Adv, 2025 Mar 11;7(6):1627-1635.
    PMID: 39886615 DOI: 10.1039/d4na01023j
    Laser surface alloying of Fe, Si, and C on aluminium is demonstrated using a Q-switched Nd:YAG laser as the source of energy. The fundamental wavelength of the laser beam was 1064 nm with an output energy of 100 mJ and a pulse duration of 10 ns. The exposure was conducted in repetitive mode with a frequency rate of 1 Hz. The laser was focused to induce plasma formation. A pure aluminium plate was employed as the substrate to be alloyed. Iron (Fe) and ceramic material silicon carbide SiC were selected as the alloy elements. Two step deposition techniques were employed to predeposit the aluminium substrate. The substrate was painted with a cohesive material gum before powder spray coating on it. The predeposited aluminium was then exposed to a focused laser at various numbers of pulses (1-13 pulses). The resulting materials were examined via scanning electron microscopy (SEM), X-ray diffraction (XRD), and microhardness techniques, revealing the formation of a homogenized resolidified surface. The plasma temperature was much higher than the melting point of Fe and SiC, enabling an immediate interaction with coating materials. The different melting points of Fe, SiC, and Al allowed the formation of a new composite during quenching. The formation of such a new composite is identified via XRD analysis. Inherently, several new composites were revealed, such as Al-Fe-Si, SiAl, and Fe-Si, with enhanced mechanical strength. Apparently, the hardness of the modified surface is confirmed to be two times greater than that of the original substrate. The sensitivity of the MSM photodetector (PD) made of the resulting alloy is reasonably high and increases with increasing the bias voltage. The response times (T Res) of the MSM PD for various numbers of laser pulses (1-13 pulses) were 0.60 s, 0.28 s, and 0.67 s with corresponding recovery times (T Rec) of 0.53 s, 0.21 s, and 1.81 s, respectively.
  10. Srikrajang S, Kabir L, Sagadevan S, Wijaya K, Oh WC
    J Mater Chem B, 2025 Feb 26;13(9):2912-2951.
    PMID: 39886804 DOI: 10.1039/d4tb02478h
    MXenes are a class of 2D transition metal carbides and nitrides (Mn+1XnT) that have attracted significant interest owing to their remarkable potential in various fields. The unique combination of their excellent electromagnetic, optical, mechanical, and physical properties have extended their applications to the biological realm as well. In particular, their ultra-thin layered structure holds specific promise for diverse biomedical applications. This comprehensive review explores the synthesis methods of MXene composites, alongside the biological and medical design strategies that have been employed for their surface engineering. This review delves into the interplay between these strategies and the resulting properties, biological activities, and unique effects at the nano-bio-interface. Furthermore, the latest advancements in MXene-based biomaterials and medicine are systematically summarized. Further discussion on MXene composites designed for various applications, including biosensors, antimicrobial agents, bioimaging, tissue engineering, and regenerative medicine, are also provided. Finally, with a focus on translating research results into real-world applications, this review addresses the current challenges and exciting future prospects of MXene composite-based biomaterials.
    MeSH terms: Animals; Humans; Biosensing Techniques; Tissue Engineering
  11. Najafi P, Motl RW, Moghadasi M
    Mult Scler Relat Disord, 2025 Jan;93:106225.
    PMID: 39709696 DOI: 10.1016/j.msard.2024.106225
    BACKGROUND: Multiple sclerosis (MS) profoundly influences fatigue, depression, various physical and mental symptoms, and quality of life (QoL). Rehabilitation, including exercise training, has improved outcomes of MS, yet is often undertaken in facilities with direct supervision which present substantial barriers for accessibility and scalability. The delivery of exercise remotely via technology (tele-exercise) might overcome those barriers and improve outcomes in MS. This systematic review and meta-analysis examined the effect of tele-exercise for improving fatigue, depression, and overall health outcomes in MS patients.

    METHOD: We undertook a comprehensive literature search across 5 electronic databases (PubMed, Scopus, Web of Science, The Cochrane Library, clinicaltrials.gov, and EMBASE) from inception through February 2024. Three reviewers screened all randomized controlled trials (RCTs) and assessed quality, and two reviewers extracted data. The meta-analysis used standardized mean difference (SMD) with Hedges' g method, a random effects model adjusted by Hartung-Knapp, and assessed heterogeneity (I² statistic), weighted studies (inverse variance), and evaluated publication bias (Begg's funnel plot and linear regression test).

    RESULT: We located 13 RCTs for inclusion in the meta-analysis. Tele-exercise interventions demonstrated significant improvements across all outcomes: depression (SMD=-0.51, p < 0.001), fatigue (SMD=-0.58, p = 0.01), physical health (SMD=0.62, p = 0.001), QoL (SMD=0.38, p = 0.02), and mental health (SMD=-0.48, p = 0.001). Mind-Body Training consistently had larger effects than Combination Training.

    CONCLUSION: Tele-exercise can improve fatigue, depression, mental and physical health, and overall QoL in MS patients. Further research is necessary to optimize tele-exercise protocols, assess long-term effects, and explore potential synergies with other intervention modalities such as telemedicine.

    MeSH terms: Telerehabilitation; Humans; Quality of Life; Outcome Assessment (Health Care); Telemedicine
  12. Ja'afar MH, Ismail R, Md Isa Z, Mohd Tamil A, Ismail NH, Mat Nasir N, et al.
    Health Sci Rep, 2025 Jan;8(1):e70310.
    PMID: 39741747 DOI: 10.1002/hsr2.70310
    BACKGROUND AND AIMS: The Malaysian government has implemented various antismoking measures to reduce the incidence of unhealthy lifestyles within the population. This study analyzes the baseline data of the Prospective Urban Rural Epidemiology (PURE) study to establish the prevalence of sociodemographic factors that are associated with smoking habits among Malaysian adults.

    METHODS: This study was carried out in urban and rural communities with adults aged between 35 and 70 years using purposive sampling. Standardized questionnaires were used to assess the smoking status and sociodemographic data of the participants. Bivariate analysis and multiple logistic regression were done to determine the association between smoking status and demographic characteristics among Malaysian adults.

    RESULTS: The prevalence of smoking among adults is 23.2%. The sociodemographic factors significantly associated with active smoking status were being a younger adult (adjusted odds ratio [AOR] = 1.26, 95% CI: 1.06-1.50), being male (AOR = 24.16, 95% CI: 20.58-28.36), being Malay (AOR = 1.72, 95% CI: 1.49-1.98), being a blue-collar worker (AOR = 1.75, 95% CI: 1.48-2.06), having no formal education (AOR = 1.99, 95% CI: 1.56-2.53), being unmarried (AOR = 1.22, 95% CI: 1.02-1.48) and being of low socioeconomic status (AOR = 1.45, 95% CI: 1.14-1.84).

    CONCLUSION: Public health policies and actions on smoking reduction should emphasize those identified as high-risk sub-populations, particularly younger adults, males and those who are not yet married, have no formal education and are of low socioeconomic status.

  13. Shah R, Hanna NM, Loo CE, David M, Mafra A, Fink H, et al.
    Nat Cancer, 2025 Jan;6(1):194-204.
    PMID: 39747650 DOI: 10.1038/s43018-024-00880-4
    The coronavirus disease 2019 pandemic substantially impacted the delivery of cancer services and programs. Here we reviewed and synthesized the global scale and impact of pandemic-related delays and disruptions on cancer services, including diagnosis, diagnostic procedures, screening, treatment and supportive and palliative care. Based on data from 245 articles in 46 countries, we observed declines in the number of cancer screening participation (39.0%), diagnoses (23.0%), diagnostic procedures (24.0%) and treatment (28.0%), ranging from a 15.0% decline for radiotherapy to a 35.0% decline for systemic treatment during the pandemic compared to during the prepandemic period. Medium-human development index (HDI) category countries experienced greater reductions than high- and very-high-HDI countries. Missing data from low-HDI countries emphasize the need for increased investments in cancer surveillance and research in these settings. PROSPERO registration: CRD42022301816.
    MeSH terms: Delivery of Health Care; Humans; Palliative Care/methods; Global Health; Early Detection of Cancer/methods; Early Detection of Cancer/statistics & numerical data; Pandemics; Time-to-Treatment/statistics & numerical data
  14. Roshanov PS, Walsh MW, Garg AX, Cuerden M, Lam NN, Hildebrand AM, et al.
    Br J Anaesth, 2025 Feb;134(2):297-307.
    PMID: 39753401 DOI: 10.1016/j.bja.2024.10.039
    BACKGROUND: Optimised use of kidney function information might improve cardiac risk prediction in noncardiac surgery.

    METHODS: In 35,815 patients from the VISION cohort study and 9219 patients from the POISE-2 trial who were ≥45 yr old and underwent nonurgent inpatient noncardiac surgery, we examined (by age and sex) the association between continuous nonlinear preoperative estimated glomerular filtration rate (eGFR) and the composite of myocardial injury after noncardiac surgery, nonfatal cardiac arrest, or death owing to a cardiac cause within 30 days after surgery. We estimated contributions of predictive information, C-statistic, and net benefit from eGFR and other common patient and surgical characteristics to large multivariable models.

    RESULTS: The primary composite occurred in 4725 (13.2%) patients in VISION and 1903 (20.6%) in POISE-2; in both studies cardiac events had a strong, graded association with lower preoperative eGFR that was attenuated by older age (Pinteraction<0.001 for VISION; Pinteraction=0.008 for POISE-2). For eGFR of 30 compared with 90 ml min-1 1.73 m-2, relative risk was 1.49 (95% confidence interval 1.26-1.78) at age 80 yr but 4.50 (2.84-7.13) at age 50 yr in female patients in VISION. This differed modestly (but not meaningfully) in men in VISION (Pinteraction=0.02) but not in POISE-2 (Pinteraction=0.79). eGFR contributed the most predictive information and mean net benefit of all predictors in both studies, most C-statistic in VISION, and third most C-statistic in POISE-2.

    CONCLUSIONS: Continuous preoperative eGFR is among the best cardiac risk predictors in noncardiac surgery of the large set examined. Along with its interaction with age, preoperative eGFR would improve risk calculators.

    CLINICAL TRIAL REGISTRATION: ClinicalTrials.gov NCT00512109 (VISION) and NCT01082874 (POISE-2).

    MeSH terms: Aged; Aged, 80 and over; Female; Glomerular Filtration Rate*; Heart Diseases/epidemiology; Humans; Male; Middle Aged; Predictive Value of Tests; Risk Factors; Surgical Procedures, Operative/adverse effects; Cohort Studies; Risk Assessment/methods
  15. Selvaraj K, Lee SJ, Song KB, Yoo BK, Karuppaiah A
    Curr Pharm Des, 2025 Jan 28.
    PMID: 39878118 DOI: 10.2174/0113816128353794241225083428
    INTRODUCTION: The objective of the present study was to improve the anti-inflammatory and antibacterial activities of mastic gum resin (MGR). MGR was loaded into a phospholipid nanocarrier with or without partially hydrolyzed ginsenoside, followed by dispersion into distilled water.

    METHOD: The phospholipid nanocarrier dispersion showed significantly enhanced in-vitro release, porcine skin/ intestine permeation, and retention. When the ratio of the MGR versus partially hydrogenated ginsenoside reached 1:1 w/w in the nanocarrier composition, the in-vitro release increased 54.8-fold compared to the MGR powder suspended in the release media.

    RESULTS: Permeation of the nanocarrier dispersion through the porcine skin and intestine increased 160-fold and 42-fold, respectively, compared to permeation of the MGR powder suspension. Furthermore, the nanocarrier dispersion reduced NO production and iNOS mRNA expression in the LPS-stimulated RAW264.7 cells. MIC and MBC of the nanocarrier dispersion against P. gingivalis were 4.11 ± 1.17 and 8.22 ± 2.35 μg/mL, respectively.

    CONCLUSION: In conclusion, the anti-inflammatory and antibacterial activities of MGR were remarkably enhanced when the MGR was loaded into the nanocarrier with partially hydrolyzed ginsenoside.

  16. Swarna Nantha Y, Chung KR, Suresh HWN, Heng VKI, Yap CG, Tan HL, et al.
    Fam Pract, 2025 Jan 17;42(1).
    PMID: 39878310 DOI: 10.1093/fampra/cmaf003
    BACKGROUND: The optimal control of type 2 diabetes (T2D) is defined by the innate mastery of self-management behaviours. This study is designed to condense the lived experiences of people with T2D in relation to factors 'exterior' to themselves into a universal self-management inventory (Assessment of Self-Management Questionnaire in Diabetes Mellitus-External Reality; ASQ-DM-EX).

    METHODS: We collected responses to an online and physical survey from people living with T2D through a quantitative cross-sectional study. First, we tested the contextual validity and cultural preciseness of ASQ-DM-EX prototype through a preliminary pilot testing phase. These processes culminated in the development of a 91-item version of the questionnaire which was disseminated widely to evaluate the predictive strength of the ASQ-DM-EX.

    RESULTS: All constructs within ASQ-DM-EX showed internal consistencies within good to excellent range (Cronbach's ⍺ = 0.70-0.94), except for the Influence construct (Cronbach's ⍺ = 0.33). An increase in ASQ-DM-EX scores was associated with a reduction in HbA1c control (r = -0.17, P 

    MeSH terms: Adult; Aged; Cross-Sectional Studies; Female; Humans; Male; Middle Aged; Psychometrics; Surveys and Questionnaires; Self Care; Reproducibility of Results
  17. Chin B, Meng Lim W, Almurisi SH, Madheswaran T
    Ther Deliv, 2025 Feb;16(2):123-137.
    PMID: 39878544 DOI: 10.1080/20415990.2025.2457314
    AIM: Abemaciclib (ABE) is an anticancer drug that suffers from low bioavailability and multidrug resistance. This study aims to develop ABE-loaded solid lipid nanoparticles (ABE-SLNs), which will enhance drug solubility and lead to increased cellular uptake and enhanced cytotoxicity when delivering tumor cells.

    METHODS: Melt emulsification followed by ultrasonication was used as a method of preparation and Quality-by-Design (QbD) was utilized to optimize ABE-SLNs.

    RESULTS: The optimized ABE-SLNs consist of Precirol-ATO5 as a lipid and Brij-58 as a surfactant. The particle size, PDI value, and zeta potential of the optimized formulation were 170.4 ± 0.49 nm, 0.25 ± 0.014, and -26.4 ± 0.1 mV, respectively. It also showed sustained release behavior and a high entrapment efficiency of 79.96%. ABE-SLNs exhibited enhanced anticancer activity in the MDA-MB-231 and T47D breast cancer cell lines compared to pure ABE. In Caco-2 human colonic cell lines, ABE-SLNs also showed increased cellular uptake.

    CONCLUSION: The use of QbD to achieve high entrapment efficiency and sustained release in ABE-SLNs, coupled with enhanced cellular uptake and cytotoxicity, represents a novel approach that could set a new standard for nanoparticle-based drug delivery systems.

    MeSH terms: Cell Survival/drug effects; Drug Carriers/chemistry; Female; Humans; Liposomes; Particle Size*; Solubility; Caco-2 Cells; Cell Line, Tumor; Drug Liberation*
  18. Lin K, Eyal S, Demarchi IG, Ben-Shushan T, Auvin S, Khoo CS, et al.
    Epilepsia, 2025 Jan 29.
    PMID: 39878760 DOI: 10.1111/epi.18286
    Clinical practice guidelines (CPGs) and consensus-based recommendations (CBRs) require considerable effort, collaboration, and time-all within the constraints of finite resources. Professional societies, such as the International League Against Epilepsy (ILAE), must prioritize what topics and questions to address. Implementing evidence-based care remains a crucial challenge in clinical practice. Using rigorous processes to ensure that the best available research evidence informs health care recommendations is of the utmost importance. We aimed to develop a structured and transparent process for prioritizing future CPGs and CBRs supported by the ILAE. A multidisciplinary group of researchers and experts from the ILAE Prioritization Task Force conducted a scoping review to identify prioritization approaches for CPG and CBR development. This scoping review was reported according to the Preferred Reporting Items for Systematic reviews and Meta-Analyses extension for Scoping Reviews (PRISMA-ScR) and Cochrane recommendations. A Problem/population, Concept, and Context (PCC) strategy was applied to the literature search and selection of the studies. We searched Medline/PubMed, Embase, Web of Science, and Scopus without time or language limits. The findings were synthesized qualitatively. A consensus-based process was followed to develop a prioritization scoring tool for CPGs and another for CBRs. Thirty-nine participants, including clinicians, experts in the field, methodologists, and other relevant stakeholders, contributed to developing the final instrument (based on a 5-point Likert scale). Of 721 unique citations, 8 papers reporting prioritization approaches for guideline development were included. Based on these, we developed an initial tool with 10 criteria. It was iteratively optimized and revised by the ILAE Standards and Best Practice Council, which unanimously approved the instrument. The ILAE Executive Committee subsequently approved its final version. The ILAE Prioritization Tool is intended to standardize the prioritization processes and optimize the ILAE's use of resources to select CPGs and CBRs for endorsement.
  19. Tham HY, Chong LC, Krishnan M, Khan AM, Choi SB, Tamura T, et al.
    Arch Microbiol, 2025 Jan 29;207(2):47.
    PMID: 39878790 DOI: 10.1007/s00203-025-04242-1
    Bacteriophages produce endolysins at the end of the lytic cycle, which are crucial for lysing the host cells and releasing virion progeny. This lytic feature allows endolysins to act as effective antimicrobial alternatives when applied exogenously. Staphylococcal endolysins typically possess a modular structure with one or two enzymatically active N-terminal domains (EADs) and a C-terminal cell wall binding domain (CBD). The EADs degrade the peptidoglycan layer, leading to bacterial lysis, while the CBD binds to the specific host cell wall, and therefore, influences specificity of the endolysin. This study aimed to alter and characterize the host specificity of the CBD by exploring the impact of amino acid modifications within the CBD of a staphylococcal endolysin, Endo88. Endo88 was able to lyse Staphylococcus spp. and Enterococcus faecalis. However, despite attempts to mutate amino acids hypothesized for binding with cell wall components, the host-range was not affected but the lytic activity was severely reduced instead, although no alterations were performed on the EADs (Cysteine, histidine-dependent aminohydrolases/peptidases domain and Amidase domain). Further investigations of the CBD alone (Src homology3 domain, SH3) without the EADs suggested that binding and lytic activity may not be correlated in some cases since Endo88 and its mutants could lyse Staphylococcus epidermidis well but no binding activity was observed in the flow cytometry analysis. Molecular docking was used to gain insights on the observations for the binding and lytic activity which may help future strategies in designing enhanced engineered endolysins.
    MeSH terms: Peptidoglycan/metabolism; Protein Binding; Enterococcus faecalis/genetics; Enterococcus faecalis/metabolism; Enterococcus faecalis/virology; Viral Proteins/genetics; Viral Proteins/metabolism; Viral Proteins/chemistry; src Homology Domains; Host Specificity*
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