Browse publications by year: 2025

  1. Shah S, Engineer KS, Shah R, Shah H, Gajjar S, Kumar S, et al.
    Cureus, 2025 Feb;17(2):e78394.
    PMID: 39902253 DOI: 10.7759/cureus.78394
    Introduction Humankind is suffering from the misuse and overuse of antibiotics, leading to antibiotic resistance. At this point, probiotics, if found effective, can lead to a better future. Probiotics are nothing but a colony of living-friendly bacteria. Lactobacillus reuteri is essential for use as a probiotic among many healthy bacteria. This study aims to compare the effectiveness of probiotics in periodontal treatment. Methods One hundred smokers diagnosed with chronic generalized periodontitis were randomly assigned into two groups. Group A received a seven-day course of amoxicillin 500 mg three a day and metronidazole 500 mg twice daily, followed by 30 days of probiotics, while Group B received a placebo antibiotic for seven days and 30 days of L. reuteri probiotics (2×10⁸ CFU daily). Periodontal parameters, including plaque index (PI), gingival index (GI), probing depth (PD), attachment loss (AL), and bleeding on probing (BOP), were utilized to asses at baseline, month one, and month one. Baseline differences were adjusted using the generalized estimating equation (GEE) model. Results At month three, Group B (probiotics) showed a significantly lower PI (0.52±0.49 vs. 0.88±0.52, p<0.001) and a 23% greater reduction in PD (p=0.004) compared to Group A (antibiotics + probiotics). These findings highlight the effectiveness of probiotics alone in improving periodontal health in smokers. Conclusions Both groups showed equal effectiveness in improving periodontal health. Hence, probiotics should be preferred over antibiotics.
  2. Navarro JC, Pandian JD, Suwanwela NC, Lee TH, Tan KS, Venketasubramanian N
    Cerebrovasc Dis Extra, 2025;15(1):30-38.
    PMID: 39701055 DOI: 10.1159/000543144
    INTRODUCTION: Intracranial large artery atherosclerosis (ICAS) is a common cause of stroke in Asia. Prior epidemiological publications on ICAS have largely been single-country reports. This collaborative study involving several Asian countries aims to investigate the characteristics and outcomes of patients with stroke attributable to ICAS.

    METHODS: This is an international, multicenter, observational, prospective cohort study of patients admitted with acute ischemic stroke secondary to ICAS to stroke centers in six Asian countries. Stroke due to ICAS was diagnosed when there was a ≥50% intracranial large artery stenosis ipslateral to a non-lacunar infarct, without significant ipsilateral extracranial stenosis, cardiac cause or other mechanism found for the stroke. Data were collected on patient demographics, vascular risk factors, stroke location, and severity. Outcomes of interest were stroke recurrence and mortality at 12-month follow-up.

    RESULTS: A total of 356 patients were recruited. Mean age was 62.7 ± 13.8 years, and 39.9% were females. Mean NIHSS on admission was 9 ± 8, with majority of patients having mild (39.3%) or moderate (37.9%) strokes. Stroke recurrence was 6.7% (95% CI: 4.4-9.9%) while mortality rate was 13.2% (95% CI: 9.9-17.2%) within 1 year. The risk of stroke recurrence was associated with increasing age (odds ratio [OR]: 1.04, 95% CI: 1-1.06, p = 0.05) and hypertension (OR: 3.23, 95% CI: 1.09-9.61, p = 0.035). Mortality was associated with age (OR: 1.05, 95% CI: 1.01-1.08, p = 0.006) and NIHSS (OR: 1.12, 95% CI: 1.07-1.17, p < 0.001).

    CONCLUSIONS: This multicenter Asian study demonstrates a high risk of stroke recurrence and mortality among patients with acute stroke due to ICAS. They are associated with age (both), as well as hypertension (for recurrence) and NIHSS (for mortality). Better treatment modalities are needed to reduce the frequency of adverse outcomes in symptomatic ICAS.

    MeSH terms: Aged; Asia/epidemiology; Disability Evaluation; Female; Humans; Male; Middle Aged; Prognosis; Prospective Studies; Recurrence*; Registries*; Risk Factors; Severity of Illness Index; Time Factors; Risk Assessment
  3. Ganapathy V, Jaganathan R, Chinnaiyan M, Chengizkhan G, Sadhasivam B, Manyanga J, et al.
    Food Chem Toxicol, 2025 Feb;196:115216.
    PMID: 39736445 DOI: 10.1016/j.fct.2024.115216
    Electronic cigarettes (e-cigarettes) have emerged as a potential alternative to traditional smoking and may aid in tobacco harm reduction and smoking cessation. E-cigarette use has notably increased, especially among young non-tobacco users, raising concerns due to the unknown long-term health effects. The oral cavity is the first and one of the most crucial anatomical sites for the deposition of e-cigarette aerosols. E-cigarette aerosols contain nicotine, flavors, volatile organic compounds, heavy metals, carcinogens, and other hazardous substances. These aerosols impact the oral cavity, disrupting host-microbial interactions and triggering gingivitis and systemic diseases. Furthermore, oral inflammation and periodontitis can be caused by proinflammatory cytokines induced by e-cigarette aerosols. The toxic components of e-cigarette aerosols increase the cellular reactive oxygen species (ROS) levels, reduce antioxidant capacity, increase DNA damage, and disrupt repair processes, which may further contribute to harmful effects on oral epithelum, leading to inflammatory and pre-malignant oral epithelial lesions. In this review, we analyze the toxicological properties of compounds in e-cigarette aerosols, exploring their cytotoxic, genotoxic, and inflammatory effects on oral health and delving into the underlying molecular mechanisms. Further research is essential to understand the impact of e-cigarettes on oral health and make informed regulatory decisions based on reliable scientific evidence.
    MeSH terms: Aerosols; Animals; DNA Damage/drug effects; Humans; Nicotine/toxicity; Oral Health*; Electronic Cigarettes*
  4. Ng WM, Chong WH, Abdullah AZ, Lim J
    Langmuir, 2025 Feb 11;41(5):3572-3582.
    PMID: 39893692 DOI: 10.1021/acs.langmuir.4c04720
    Nanoscale zerovalent iron (nZVI) has been proposed as a promising nanomaterial for soil remediation. However, injecting nZVI into contaminated sites to target and treat pollutant sources may pose potential environmental risks due to its colloidal stability and mobility in the environment. In this regard, this study assessed the feasibility of implementing magnetic capture of surface-functionalized nZVI in soil environments under the influence of the convective flow current. Here, functionalized nZVI particles were prepared by introducing carboxymethyl cellulose (CMC) as a stabilizing agent during the synthesis of nZVI by using the liquid-phase reduction method. The functionalized nZVI particles were then injected into a two-dimensional flow column containing a sand matrix with a high gradient magnetic trap (HGMT) embedded within the system. Particle transports in both the absence and presence of a magnetic field were recorded by using a digital camera, and the breakthrough curves were generated from the data collected spectrophotometrically. The results showed that the relative breakthrough concentration of nZVI decreased from 0.92 to nearly zero, with a delayed breakthrough time as the applied magnetic field strength increased from zero (no magnetic field) to 0.093 T, demonstrating a 100% capture efficiency. It was found that the magnetic capture for the nZVI particles was contributed by two mechanisms: (1) low gradient magnetic separation (LGMS), driven by the penetrating magnetic field from the permanent magnets, and (2) high gradient magnetic separation (HGMS), which occurred near the wire surfaces within the HGMT section magnetized by the permanent magnets. Findings in this work have proven the feasibility of magnetic separation as a control strategy for nanoparticle applications in environmental remediation.
  5. Meunier M, Haack M, Awad D, Brück T, Awang K, Litaudon M, et al.
    Talanta, 2025 May 15;287:127626.
    PMID: 39893730 DOI: 10.1016/j.talanta.2025.127626
    The chemical profiling of complex mixtures of natural products (NPs) is a major challenge in analytical chemistry and generally addressed by liquid chromatography coupled to mass spectrometry (LC-MS). In recent years also matrix free laser desorption ionization-mass spectrometry (LDI-MS) has become a versatile and time efficient complement to LC-MS. However, the absence of chromatographic separation in LDI-MS does not permit the differentiation of isomers. Providing a potential solution to this problem, the current work presents a combined LDI-Ion mobility spectrometry-tandem mass spectrometry (LDI-IMS-MS2) approach, which facilitated the successful differentiation of four constitutional xanthone isomers namely butyraxanthone D, cratoxylone, garcinone D and parvixanthone G. In addition, the experimental collision cross section (CCS) distribution values of nine unreported xanthones are described. Based on these results, a proof of concept for the so far unexplored concept of a LDI-IMS-MS2 based molecular network is being presented.
  6. Rajendran A, Ramlal A, Harika A, Subramaniam S, Raju D, Lal SK
    Plant Physiol Biochem, 2025 Mar;220:109579.
    PMID: 39893944 DOI: 10.1016/j.plaphy.2025.109579
    An excess of water is more harmful to plant growth, root growth and the uniformity of the plant population than a water deficit. Water is a crucial factor in the three basic stages of soybean development: germination, emergence and flowering/seed filling. Waterlogging is one of the biggest constraints to crop production and productivity in India and can occur at any stage in soybean. However, seeds and seedlings are damaged by waterlogging resulting in a significant reduction in grain yield. Seed yield and growth are significantly correlated at the seedling stage. In addition, the plant is under constant pressure due to changing environmental conditions and has difficulty withstanding these harsh, unpredictable and difficult situations. Membrane transporters are essential and play fundamental roles during waterlogging thereby facilitating cellular homeostasis and gaseous exchange, which support plant growth and development. This review highlights the genetic basis and mechanism of waterlogging tolerance in soybean and the role of climate in influencing the genetic makeup of the crop, paving the way for further development of improved soybean varieties. Simultaneously, the article highlights membrane transporters' importance in water-mediated stress in soybeans.
    MeSH terms: Plant Proteins/genetics; Plant Proteins/metabolism; Stress, Physiological*; Water/metabolism
  7. Mai W, Mai L, Chen Y
    Eval Program Plann, 2025 Jun;110:102551.
    PMID: 39894001 DOI: 10.1016/j.evalprogplan.2025.102551
    Insufficient and low public service quality in China has resulted in unfulfilled resident needs, necessitating an examination to improve public service quality. Thus, this study constructs a public service quality index for 29 provinces covering 2004-2020 to explore the relationship between expenditure decentralization and public service quality. Using Fixed effects model, OLS and System Generalized Method of Moments (SYS-GMM) technique, this study reveals that expenditure decentralization has a significantly positive effect on public service quality, with a 1 % increase in expenditure decentralization leading to a 0.287 % improvement in public service quality. Additionally, the impact of expenditure decentralization on public service quality in the eastern and central regions is positively significant, while in the western region, expenditure decentralization does not positively affect public service quality. Furthermore, based on the public service quality theory, this study finds that FDI and fiscal self-sufficiency can enhance the relationship between expenditure decentralization and public service quality. The study provides evidence-based recommendations for policymakers to prioritize expenditure decentralization as a strategy to enhance public service quality, especially when considering attracting FDI and promoting fiscal self-sufficiency.
    MeSH terms: China; Humans; Politics*
  8. Swami V, Voracek M, Furnham A, Horne G, Longhurst P, Tran US
    Body Image, 2025 Mar;52:101854.
    PMID: 39894002 DOI: 10.1016/j.bodyim.2025.101854
    A growing body of evidence suggests that exposure to natural environments is associated with more positive body image, but such work has invariably centred the experiences of neurotypical adults and bodies. To rectify this oversight, we examined whether direct and indirect (i.e., mediational) pathways between nature exposure and an index of positive body image (i.e., body appreciation) are significant in autistic adults. A total of 303 autistic adults (age M = 36.69, range 18-75 years) from the United Kingdom completed an online survey that included measures of nature exposure, body appreciation, self-compassion, and nature connectedness, as well as sociodemographic variables. Structural equation modeling was used to test a hypothesised parallel mediation model in which self-compassion and connectedness to nature, respectively, mediated the association between nature exposure and body appreciation. Results showed that connectedness to nature, but not self-compassion, mediated the relationship between nature exposure and body appreciation. This finding was robust to sensitivity analyses and consistent across participants who identified as women and men. These results suggest that nature exposure is associated with more positive body image in autistic adults, which practitioners may find useful in designing population-specific nature-based interventions.
    MeSH terms: Adolescent; Adult; Aged; Empathy; Environment; Female; Great Britain; Humans; Male; Middle Aged; Personal Satisfaction; Surveys and Questionnaires; Self Concept*; Young Adult
  9. de Sousa DS, de Almeida FHO, Gonçalves GOS, Leite AROF, Martins-Filho PR, da Silva FA
    Am J Pharm Educ, 2025 Jan 31.
    PMID: 39894253 DOI: 10.1016/j.ajpe.2025.101365
    OBJECTIVES: This study aims to identify and evaluate instruments used to measure pharmacy students' knowledge of HIV prophylaxis, focusing on both Pre-Exposure Prophylaxis (PrEP) and Post-Exposure Prophylaxis (PEP). Understanding these educational tools is essential for improving knowledge in health promotion, prevention, diagnosis, treatment, and management.

    METHODS: A systematic literature search was conducted using PubMed, Virtual Health Library, Web of Science, Scopus, Science Direct, and Embase. Article screening was independently performed by two reviewers. Studies assessing pharmacy students' knowledge of HIV prophylaxis were included. Data were extracted and organized into two categories: study characteristics (author, year, country, objectives, design, sample size, duration, and outcomes) and instrument characteristics (authors, year, country, instrument name, objective, description, type of assessment, mode of assessment, validation processes, and experimental phase).

    RESULTS: Eight studies were identified, including one from Malaysia and seven from the United States, involving 1,797 students. All studies employed cross-sectional designs, and only one assessed knowledge retention through an intervention. Eight assessment instruments were identified: six focused on PrEP, one on PEP, and one on both. Some studies demonstrated substantial knowledge and confidence in PrEP, but significant gaps were found in PEP awareness, access to prophylaxis, and familiarity with prescribing guidelines.

    CONCLUSIONS: There is a notable shortage of assessment tools for PEP. Further research is needed to develop validated instruments that measure knowledge and address educational gaps. Longitudinal studies with pre- and post-tests are crucial to evaluate educational interventions and enhance pharmacy students' preparedness for HIV prophylaxis.

  10. Arya DK, Pandey P, Kumar A, Chidambaram K, Fatease AA, Pandey G, et al.
    J Liposome Res, 2025 Feb 02.
    PMID: 39895032 DOI: 10.1080/08982104.2025.2457453
    Personalized treatment strategies have greatly improved the efficacy of anticancer drugs. Nanocarriers, especially liposomes, function as excellent platform for the delivery of both hydrophilic and hydrophobic agents. iRGD is a peptide composed of 9-amino acid denoted as (iRGDP), enhances selective and intratumoral delivery of anticancer drugs. Trastuzumab (TMAB), mainly targets HER2-positive advanced stage breast cancer is an FDA-approved monoclonal antibody. Gefitinib (GEB) is an anticancer drug, effective against metastatic breast cancer (MBC), while Lycorine hydrochloride (LCOH), a naturally derived compound, possess both anti-inflammatory and anticancer properties. This research is mainly emphasizing on the preparation of GEB and LCOH-entrapped TPGS-COOH coated-liposomes, camouflaged with an antibody (TMAB) and cyclic peptide (iRGDP) for targeted delivery in MBC therapy. The developed multifunctional liposomes were studied for extensive in vitro cell line studies on MCF-7 cells. The half-maximum inhibitory concentration (IC-50) values of GEB and LCOH co-loaded single functionalized liposome (SFL) (iRGDP-LiP, and TMAB-LiP) and dual-functionalized liposome (DFL) (iRGDP-TMAB-LiP) on MCF-7 cells were 1.04 ± 0.023 μg/mL, 0.71 ± 0.018 μg/mL, and 0.56 ± 0.028 μg/mL, respectively. Inverted confocal laser scanning microscopy (ICLSM) revealed enhanced cellular internalization in SFL and DFL-treated groups tagged with coumarin-6 and rhodamine-B dye as compared to conventional liposome. The scratch assay revealed a marked reduction in cell migration, while DAPI staining confirmed enhanced nuclear condensation (NC) and nuclear fragmentation (NF) in SFL and DFL-treated groups. Moreover, flow cytometry demonstrated enhanced early and late apoptosis in SFL and DFL groups. These findings indicate that GEB and LCOH co-loaded multifunctional liposome holds promise as a multifaceted therapeutic approach for MBC therapy.
  11. Jose S, Sharma H, Insan J, Sharma K, Arora V, Puranapanda S, et al.
    Mol Cell Biol, 2025 Feb 02.
    PMID: 39895059 DOI: 10.1080/10985549.2025.2454421
    Pyridinyl-imidazole class p38 MAPKα/β (MAPK14/MAPK11) inhibitors including SB202190 have been shown to induce cell-type specific defective autophagy resulting in micron-scale vacuole formation, cell death, and tumor suppression. We had earlier shown that this is an off-target effect of SB202190. Here we provide evidence that this vacuole formation is independent of ATG5-mediated canonical autophagosome initiation. While SB202190 interferes with autophagic flux in many cell lines parallel to vacuolation, autophagy-deficient DU-145 cells and CRISPR/Cas9 gene-edited ATG5-knockout A549 cells also undergo vacuolation upon SB202190 treatment. Late-endosomal GTPase RAB7 colocalizes with these compartments and RAB7 GTP-binding is essential for SB202190-induced vacuolation. A screen for modulators of SB202190-induced vacuolation revealed molecules including multi-kinase inhibitor sorafenib as inhibitors of vacuolation and sorafenib co-treatment enhanced cytotoxicity of SB202190. Moreover, VE-821, an ATR inhibitor was found to phenocopy the cell-type specific vacuolation response of SB202190. To identify the factors determining the cell-type specificity of vacuolation induced by SB-compounds and VE-821, we compared the transcriptomics data from vacuole-forming and non-vacuole-forming cancer cell lines and identified a gene expression signature that may define sensitivity of cells to these small-molecules. Further analyses using small molecule tools and the gene signature discovered here, could reveal novel mechanisms regulating this interesting anti-cancer phenotype.
  12. Sadikan MZ, Lambuk L, Reshidan NH, Abdul Ghani NA, Ahmad AI, Ahmad Kamal MS, et al.
    J Ocul Pharmacol Ther, 2025 Apr;41(3):150-161.
    PMID: 39895321 DOI: 10.1089/jop.2024.0123
    Age-related macular degeneration (AMD) poses a significant threat to visual health among the elderly, necessitating urgent preventive measures as the global population ages. Extensive research has implicated oxidative stress (OS)-induced retinal damage as a primary contributor to AMD pathogenesis, prompting investigations into potential therapeutic interventions. Among the various nutrients studied for their potential in AMD risk reduction, antioxidants have shown promise, with initial findings from the Age-Related Eye Disease Study suggesting a correlation between antioxidant supplementation and decreased AMD progression. This article explores the scientific foundation supporting the therapeutic efficacy of tocotrienol-rich fraction (TRF) as a viable candidate for slowing AMD progression, based on interventional studies. AMD is characterized by OS, inflammation, dysregulated lipid metabolism, and angiogenesis, all of which TRF purportedly addresses through its potent anti-inflammatory, lipid-lowering, antiangiogenic, and antioxidant properties. The review underscores TRF's promising attributes, aiming to deepen understanding of AMD pathogenesis and advocate for TRF-based pharmacological interventions to enhance therapeutic outcomes. Given the pressing need for effective AMD treatments, TRF represents a promising avenue for intervention, offering hope for improved vision outcomes and enhanced quality of life for individuals affected by this debilitating condition.
    MeSH terms: Animals; Humans; Disease Progression
  13. Tan S, Teh LJ, Mohd Mokhtaruddin S, Henry Basil J
    Int J Qual Health Care, 2025 Feb 18;37(1).
    PMID: 39895336 DOI: 10.1093/intqhc/mzaf012
    BACKGROUND: Medication administration in busy emergency departments (EDs) presents significant challenges due to the high-pressure environment and complexity of processes. Medication administration errors (MAEs) are a critical concern as they can severely impact patient safety and increase healthcare costs. Understanding the prevalence and underlying factors contributing to intravenous MAEs is essential for enhancing patient care and improving hospital services. This study aims to explore and measure the frequency of intravenous MAEs in EDs and identify factors contributing to these errors during their preparation and administration.

    METHODS: This prospective study utilized the direct observation technique where the preparation and administration of 222 intravenous medications were observed in the EDs of two hospitals in Malaysia. Information on medication preparation, administration, and other procedures was recorded. Error rates were calculated, and multivariable logistic regression was conducted to identify factors contributing to intravenous MAEs.

    RESULTS: MAEs were detected in 83.3% (185/222) of the observed medications affecting 86.7% (124/143) patients. Among these, a total of 240 MAEs were identified, with the most common being wrong rate of administration (55.8%), wrong preparation technique (20.8%), and omission error (11.7%). Alimentary tract and metabolism medications accounted for the highest proportion of MAEs (52.0%), followed by anti-infective medications (21.7%) and nervous system medications (15.4%). Excluding wrong time errors reduced the error rate to 80.2% (178/222). Nonverbal orders and inadequate or absence of labelling were significantly associated with MAEs, while factors such as the complexity of preparations, working shift, experience, and high-alert medications showed no significant associations.

    CONCLUSION: The study highlighted a high prevalence of intravenous MAEs in EDs. Nonverbal orders and inadequacy in labelling of medications were significant contributing factors to MAEs in the ED. Implementing an admixture labelling policy, comprehensive training programmes, strict enforcement of existing guidelines and protocols through regular audits, establishing nonpunitive error reporting system, and technological solutions where financially feasible are crucial for mitigating these errors to promote patient safety.

    MeSH terms: Adult; Aged; Female; Humans; Malaysia; Male; Middle Aged; Prospective Studies; Prevalence; Patient Safety; Administration, Intravenous*
  14. Chin JW, Loh WML, Ooi YBH, Khor BH
    Nutr Bull, 2025 Feb 03.
    PMID: 39895580 DOI: 10.1111/nbu.12727
    Recently, there has been an emerging trend of purchasing foods and beverages via online food delivery systems but there is scarce evidence on the healthfulness of these items, particularly in Southeast Asian countries such as Malaysia. This study aimed to evaluate the characteristics and nutrient profiles of foods and beverages available via online food delivery systems in Malaysia. This cross-sectional study was conducted from September 2022 to March 2023 to identify foods and beverages available on the Grab Food and Foodpanda mobile applications. The healthfulness of selected foods and beverages was determined based on the Food Standards Agency Nutrient Profiling System. The present study included 3729 foods and 1882 beverages. Most of the foods were cereal-based dishes (37.4%), followed by cereals with protein-based dishes (12.8%) and meat or poultry-based dishes (12.0%), while most of the beverages were local handcrafted beverages (27.8%), followed by bubble milk tea (15.0%) and Western handcrafted beverages (14.6%). For protein dishes, deep-frying or battered-frying was the most common preparation method (33.8%) while most of the cereal-based dishes were stir-fried (76.7%). Out of 23 common foods, 15 foods (65%) were categorised as less healthy based on the nutrient profile score while 19 out of 24 (79%) common beverages were categorised as less healthy. The online food delivery systems feature predominantly local foods and beverages that are less healthy, potentially contributing to the development of an obesogenic environment.
  15. El Imene Bennoui RN, Adli W, Al-Douri Y, Belkharroubi F, Sidjilani F, Bentayeb A, et al.
    Chemphyschem, 2025 Feb 03.
    PMID: 39895590 DOI: 10.1002/cphc.202400921
    The full-potential linearized augmented plane wave (FP-LAPW) method within the framework of density functional theory (DFT) and semi-classical Boltzmann transport theory under the constant relaxation time approximation has been employed to investigate the structural, mechanical, optoelectronic and thermoelectric properties of novel half-Heusler (HH) ZrYAu alloys (where Y=B or Al) with a valence electron count (VEC) of 8. Our results indicate that both compounds are mechanically stable in structure Type 1 and possess negative formation energies. Additionally, ZrBAu and ZrAlAu display semiconducting behavior, with ZrBAu showing a direct band gap, 0.753 eV (0.774 eV) at point Γ→X and ZrAlAu exhibiting an indirect band gap, 0.431 eV (0.482 eV) at point Γ→Γ, using the generalized gradient approximation (GGA) and Modified Becke and Johnson-generalized gradient approximation (mBJ-GGA), respectively. Based on optical properties, both ZrBAu and ZrAlAu exhibit high optical conductivity within the visible spectrum. In terms of visible light absorption, ZrBAu primarily absorbs blue light, while ZrAlAu absorbs yellow, blue-green and violet light. However, both compounds are effective absorbers of UV light. Regarding thermoelectric performance, the thermoelectric parameters reveal that ZrBAu and ZrAlAu demonstrate significant p-type thermoelectric power. These half-Heusler alloys have a high-power factor, making them promising candidates for thermoelectric applications.
  16. Zakaria Z, Mazlan M, Chung TY, Selvanayagam VS, Temesi J, Magenthran V, et al.
    Biomed Tech (Berl), 2025 Feb 25;70(1):3-10.
    PMID: 39320241 DOI: 10.1515/bmt-2023-0501
    Mechanomyography (MMG) may be used to quantify very small motor responses resulting from muscle activation, voluntary or involuntary. The purpose of this study was to investigate the MMG mean peak amplitude (MPA) and area under the curve (AUC) and the corresponding mechanical responses following delivery of transcranial magnetic stimulation (TMS) to the knee extensors. Fourteen adults (23 ± 1 years) received single TMS pulses at intensities from 30-80 % maximum stimulator output to elicit muscle responses in the relaxed knee extensors while seated. An accelerometer-based sensor was placed on the rectus femoris (RF) and vastus lateralis (VL) muscle bellies to measure the MMG signal. Pearson correlation revealed a positive linear relationship between MMG MPA and TMS intensity for RF (r=0.569; p<0.001) and VL (r=0.618; p<0.001). TMS intensity of ≥60 % maximum stimulator output produced significantly higher MPA than at 30 % TMS intensity and evoked measurable movement at the knee joint. MMG MPA was positively correlated to AUC (r=0.957 for RF and r=0.603 for VL; both p<0.001) and knee extension angle (r=0.596 for RF and r=0.675 for VL; both p<0.001). In conclusion, MMG captured knee extensor mechanical responses at all TMS intensities with the response increasing with increasing TMS intensity. These findings suggest that MMG can be an additional tool for assessing muscle activation.
    MeSH terms: Adult; Electromyography/methods; Humans; Knee Joint/physiology; Evoked Potentials, Motor/physiology; Young Adult
  17. Reilly JJ, Andrew R, Abdeta C, Azevedo LB, Farias NA, Barak S, et al.
    Sports Med, 2025 Jan;55(1):203-219.
    PMID: 39361231 DOI: 10.1007/s40279-024-02104-2
    BACKGROUND: The actions required to achieve higher-quality and harmonised global surveillance of child and adolescent movement behaviours (physical activity, sedentary behaviour including screen time, sleep) are unclear.

    OBJECTIVE: To identify how to improve surveillance of movement behaviours, from the perspective of experts.

    METHODS: This Delphi Study involved 62 experts from the SUNRISE International Study of Movement Behaviours in the Early Years and Active Healthy Kids Global Alliance (AHKGA). Two survey rounds were used, with items categorised under: (1) funding, (2) capacity building, (3) methods, and (4) other issues (e.g., policymaker awareness of relevant WHO Guidelines and Strategies). Expert participants ranked 40 items on a five-point Likert scale from 'extremely' to 'not at all' important. Consensus was defined as > 70% rating of 'extremely' or 'very' important.

    RESULTS: We received 62 responses to round 1 of the survey and 59 to round 2. There was consensus for most items. The two highest rated round 2 items in each category were the following; for funding (1) it was greater funding for surveillance and public funding of surveillance; for capacity building (2) it was increased human capacity for surveillance (e.g. knowledge, skills) and regional or global partnerships to support national surveillance; for methods (3) it was standard protocols for surveillance measures and improved measurement method for screen time; and for other issues (4) it was greater awareness of physical activity guidelines and strategies from WHO and greater awareness of the importance of surveillance for NCD prevention. We generally found no significant differences in priorities between low-middle-income (n = 29) and high-income countries (n = 30) or between SUNRISE (n = 20), AHKGA (n = 26) or both (n = 13) initiatives. There was a lack of agreement on using private funding for surveillance or surveillance research.

    CONCLUSIONS: This study provides a prioritised and international consensus list of actions required to improve surveillance of movement behaviours in children and adolescents globally.

    MeSH terms: Adolescent; Child; Delphi Technique*; Female; Humans; Sleep; Global Health; Exercise*; Consensus; Sedentary Lifestyle*; Capacity Building
  18. Suppiah J, Md Sani SS, Hassan SS, Nadzar NIF, Ibrahim N', Thayan R, et al.
    Virus Genes, 2025 Feb;61(1):26-37.
    PMID: 39397194 DOI: 10.1007/s11262-024-02114-2
    Dengue virus hijacks host cell mechanisms and immune responses in order to replicate efficiently. The interaction between the host and the virus affects the host's gene expression, which remains largely unexplored. This pilot study aimed to profile the host transcriptome as a potential strategy for identifying specific biomarkers for dengue prediction and detection. High-throughput RNA sequencing (RNA-seq) was employed to generate host transcriptome profiles in 16 dengue patients and 10 healthy controls. Differentially expressed genes (DEGs) were identified in patients with severe dengue and those with dengue with warning signs compared to healthy individuals. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses were performed to elucidate the functions of upregulated and downregulated genes. Compared to healthy controls, 6466 genes were significantly differentially expressed (p 
    MeSH terms: Adult; Female; Humans; Male; Middle Aged; Biomarkers; Sequence Analysis, RNA*; Gene Expression Profiling; High-Throughput Nucleotide Sequencing; Transcriptome/genetics; Gene Ontology
  19. Thwaites L, Nasa P, Abbenbroek B, Dat VQ, Finfer S, Kwizera A, et al.
    Intensive Care Med, 2025 Jan;51(1):21-38.
    PMID: 39714613 DOI: 10.1007/s00134-024-07735-7
    PURPOSE: To generate consensus and provide expert clinical practice statements for the management of adult sepsis in resource-limited settings.

    METHODS: An international multidisciplinary Steering Committee with expertise in sepsis management and including a Delphi methodologist was convened by the Asia Pacific Sepsis Alliance (APSA). The committee selected an international panel of clinicians and researchers with expertise in sepsis management. A Delphi process based on an iterative approach was used to obtain the final consensus statements.

    RESULTS: A stable consensus was achieved for 30 (94%) of the statements by 41 experts after four survey rounds. These include consensus on managing patients with sepsis outside a designated critical care area, triggers for escalating clinical management and criteria for safe transfer to another facility. The experts agreed on the following: in the absence of serum lactate, clinical parameters such as altered mental status, capillary refill time and urine output may be used to guide resuscitation; special considerations regarding the volume of fluid used for resuscitation, especially in tropical infections, including the use of simple tests to assess fluid responsiveness when facilities for advanced hemodynamic monitoring are limited; use of Ringer's lactate or Hartmann's solution as balanced salt solutions; epinephrine when norepinephrine or vasopressin are unavailable; and the administration of vasopressors via a peripheral vein if central venous access is unavailable or not feasible. Similarly, where facilities for investigation are unavailable, there was consensus for empirical antimicrobial administration without delay when sepsis was strongly suspected, as was the empirical use of antiparasitic agents in patients with suspicion of parasitic infections.

    CONCLUSION: Using a Delphi method, international experts reached consensus to generate expert clinical practice statements providing guidance to clinicians worldwide on the management of sepsis in resource-limited settings. These statements complement existing guidelines where evidence is lacking and add relevant aspects of sepsis management that are not addressed by current international guidelines. Future studies are needed to assess the effects of these practice statements and address remaining uncertainties.

    MeSH terms: Adult; Critical Care/methods; Critical Care/standards; Delphi Technique*; Developing Countries; Health Resources; Humans; Consensus*
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