Browse publications by year: 2025

  1. Yee MM, Chin KY, Ima-Nirwana S, Alias E, Chua KH, Wong SK
    Int J Med Sci, 2025;22(3):585-603.
    PMID: 39898246 DOI: 10.7150/ijms.103445
    Background: Carotene exists naturally in a complex mixture consisting of alpha (α), beta (β), and gamma (γ)-isoforms. Previous studies investigated the effects of individual carotene isomers on bone rather than their actions in a mixture. Purpose: This study explored the bone-protective properties of palm carotene mixture using both two- and three-dimensional co-culture systems. Study design: The viability of human foetal osteoblasts (hFOB 1.19), viability of human monocytic cell line (THP-1), osteoblast differentiation, osteoclast maturation, bone quality and strength were assessed in two- and three-dimensional co-culture system after treatment of palm carotene mixture. Methods: The viability of hFOB 1.19 and THP-1 was determined on day 1, 3, and 6 following treatment of palm carotene mixture. The osteoblast-osteoclast co-culture (ratio of hFOB 1.19 to THP-1 = 2:1) was treated with palm carotene mixture as well as subjected to alkaline phosphatase (ALP) and tartrate resistant acid phosphatase (TRAP) staining on day 21 to assess the osteoblast proliferation and osteoclast maturation. Dual-energy X-ray absorptiometry, micro-computed tomography, universal testing machine, and bone histomorphometry were used to assess the bone parameters of scaffolds co-cultured with osteoblasts and osteoclasts. Results: Palm carotene mixture (3.13 - 50 μg/mL) increased osteoblast viability. Monocyte viability decreased in lower concentration (3.13 - 12.5 μg/mL) but increased in higher concentration (25 - 50 μg/mL) of palm carotene mixture. Treatment with palm carotene mixture (12.5 µg/mL) demonstrated earlier peak for the ALP-positive area on day 14 but decreased total number of TRAP-positive multinucleated cells on day 21. Palm carotene mixture also increased bone volume and osteoblast number in the three-dimensional co-culture system. Conclusion: Palm carotene mixture potentially exhibits beneficial effects on bone by accelerating osteoblast proliferation and suppressing osteoclast maturation. The findings of current study serve as the basis for the further validation through animal experiments and human trials.
    MeSH terms: Bone and Bones/cytology; Bone and Bones/drug effects; Cell Survival/drug effects; Humans; Osteogenesis/drug effects; Coculture Techniques*; Arecaceae/chemistry; Cell Proliferation/drug effects
  2. Roberts JA, Sime FB, Lipman J, Hernández-Mitre MP, Baptista JP, Brüggemann RJ, et al.
    Intensive Care Med, 2025 Feb;51(2):302-317.
    PMID: 39899034 DOI: 10.1007/s00134-025-07793-5
    PURPOSE: Appropriate antifungal therapy is a major determinant of survival in critically ill patients with invasive fungal disease. We sought to describe whether contemporary dosing of antifungals achieves therapeutic exposures in critically ill patients.

    METHODS: In a prospective, open-label, multicenter pharmacokinetic study, intensive care unit (ICU) patients prescribed azoles, echinocandins, or polyene antifungals for treatment or prophylaxis of invasive fungal disease were enrolled. Blood samples were collected on two occasions, with three samples taken during a single dosing interval on each occasion. Total concentrations were centrally measured using validated chromatographic methods. Pharmacokinetic parameters were estimated using noncompartmental methods. Antifungal dosing adequacy was assessed using predefined PK/PD targets.

    RESULTS: We included 339 patients from 30 ICUs across 12 countries. Median age 62 (interquartile range [IQR], 51-70) years, median APACHE II score 22 (IQR, 17-28), and 61% males. Antifungal therapy was primarily prescribed for treatment (80.8%). Fluconazole was the most frequently prescribed antifungal (40.7%). The most common indication for treatment was intra-abdominal infection (30.7%). Fungi were identified in 45% of patients, of which only 26% had a minimum inhibitory concentration available. Target attainment was higher for patients receiving prophylaxis (> 80% for most drugs). For patients receiving treatment, low target attainment was noted for voriconazole (57.1%), posaconazole (63.2%), micafungin (64.1%) and amphotericin B (41.7%).

    CONCLUSION: This study highlights the varying degrees of target attainment across antifungal agents in critically ill patients. While a significant proportion of patients achieved the predefined PK/PD targets, wide variability and subtherapeutic exposures persist.

    TRIAL REGISTRATION: ClinicalTrials.gov Identifier: NCT03136926, 2017-04-21.

    MeSH terms: Aged; Female; Humans; Male; Middle Aged; Prospective Studies; Fluconazole/administration & dosage; Fluconazole/pharmacokinetics; Fluconazole/therapeutic use; Critical Illness*; Echinocandins/administration & dosage; Echinocandins/pharmacokinetics; Echinocandins/therapeutic use
  3. Al-Towayti FAH, Teh HM, Ma Z, Jae IA, Syamsir A
    PLoS One, 2025;20(2):e0313955.
    PMID: 39899504 DOI: 10.1371/journal.pone.0313955
    Mangrove ecosystems and other coastal protection structures are essential barriers protecting coastal populations from the damaging effects of wave energy and increasing sea levels. This study uses a semicircular breakwater (SBW) model in an effort to develop coastal protection measures. The hydrodynamic characteristics of the SBW under random wave conditions, including the transmission coefficient, reflection coefficient, and energy loss coefficient, were thoroughly investigated using physical model experimentation. The main objectives encompass understanding the behavior of the SBW model, developing empirical equations to estimate hydraulic characteristics, and enhancing coastal protection structures to facilitate the preservation and expansion of mangrove ecosystems. Hydrodynamic features of the SBW model were assessed across a spectrum of wave conditions. Experimental testing in a wave flume encompassed a range of relative water depths (d/h), including d/h = 0.667 for an emerged SBW, d/h = 1.000 for an alternatively submerged SBW, and fully submerged conditions for d/h = 1.333 and 1.667. Wave steepness (Hi/L) varied from 0.02 to 0.06, and wave periods ranged from 0.8 to 2.5 seconds. Notably, analysis of an emerged SBW with d/h = 0.667 revealed superior wave attenuation compared to d/h = 1.000, 1.333, and 1.667 configurations.
    MeSH terms: Models, Theoretical; Water Movements; Ecosystem; Hydrodynamics*
  4. Hamza MN, Tariqul Islam M, Lavadiya S, Ud Din I, Sanches B, Koziel S, et al.
    PLoS One, 2025;20(2):e0311431.
    PMID: 39899558 DOI: 10.1371/journal.pone.0311431
    Cervical cancer belongs to the most dangerous types of cancers posing considerable threat to women's survival. It is most often diagnosed in the advanced stages as precancerous lesions are often symptom-free and difficult to identify. Microwave imaging, especially in terahertz (THz) range, is a convenient and noninvasive cancer detection tool. It enables characterization of biological tissues and discrimination between healthy and malignant ones. This study presents a novel triple-band biosensor based on metamaterials (MTMs). By leveraging unique properties of MTMs, the proposed biosensor operates as a perfect absorber. It exploits resonant modes in the THz spectrum to achieve remarkable sensitivity. Meticulous selection of the sensor geometry and dimensions enables efficient miniaturization. Meanwhile, utilization of frequency-domain data to detect refractive index changes improves resolution of cancerous tissue identification. Extensive numerical investigations corroborate its ability to carry out reliable early-stage cervical cancer diagnosis. This includes identification of the spatial extent of the malignant tissue. Excellent electrical properties of the sensor are accompanied by its compact size, which is highly desirable for non-invasive and portable applications.
    MeSH terms: Female; Humans
  5. Mohd Hardy Abdullah NA, Dom NC, Pradhan B, Salleh SA, Dapari R
    PLoS One, 2025;20(2):e0316564.
    PMID: 39899560 DOI: 10.1371/journal.pone.0316564
    INTRODUCTION: Dengue continues to be a major public health concern in Malaysia, as evidenced by the significant surge in cumulative dengue case numbers and deaths in 2023 compared to the previous year. While previous studies have explored the interplay of abiotic and biotic factors of mosquito density and dengue cases on a local scale in Malaysia, there is a notable gap in the research focusing on adult Aedes mosquito populations.

    AIMS: This study aims to contribute to the existing knowledge by investigating the association and time lags (TLs) between daily microclimate (DM), mosquito indices (MIs), and dengue cases at the residence level.

    METHODS: In this longitudinal study, field data were collected over 26 weeks using data loggers, gravid oviposit sticky (GOS) traps, and non-structural 1 (NS1) test kits in both non-dengue hotspot (NDH) and dengue hotspots (DH). The collected data encompassed DM variables, vegetation cover (VC), MIs, and number of dengue cases. An autocorrelation analysis was conducted to determine the TLs between MIs and their preceding values, while a cross-correlation analysis revealed the TLs between MIs and DM variables.

    RESULTS: The study indicated there are positive correlations between the adult index (AI) of Ae. albopictus, their preceding values and rainfall at an NDH. Conversely, the AIs of total Aedes at the DH exhibited positive correlations with their preceding values, temperature, rainfall, and maximum relative humidity (RH), but negative correlations with the mean and maximum RH. The dengue-positive trap index (DPTI) of total Aedes at DHs demonstrated positive associations with their preceding values, mean temperature, minimum temperature, maximum RH, and rainfall, with negative correlations observed for the maximum temperature, mean RH, and minimum RH. Similar trends were identified for the Ae. aegypti and Ae. albopictus at DHs. The association between dengue cases, DM, and MIs was inconclusive due to underreported cases.

    CONCLUSIONS: This study highlighted the DM and TLs of dengue virus-infected and non-infected adult female Aedes mosquitoes using onsite data collection. Furthermore, this study presents a replicable methodology that can be adopted by researchers worldwide for investigating the dynamics of dengue transmission in similar settings. The findings offer valuable insights for decision-makers, providing them with evidence-based information to implement targeted interventions and strategies aimed at controlling Aedes mosquito populations and mitigating the spread of dengue virus infections.

    MeSH terms: Animals; Dengue Virus; Female; Humans; Longitudinal Studies; Malaysia/epidemiology; Microclimate*; Population Density; Temperature
  6. Zafar R, Rehman IU, Shah Y, Ming LC, Goh KW, Suleiman AK, et al.
    PLoS One, 2025;20(2):e0317734.
    PMID: 39899613 DOI: 10.1371/journal.pone.0317734
    INTRODUCTION: Chronic kidney disease (CKD) patients suffer from different comorbid conditions and are prone toward drug-related problems (DRPs) which affect their clinical parameters as well as quality of life (QoL). This study was aimed to evaluate the impact of clinical pharmacist-led interventions on the mean number of DRPs and the mean QoL score difference per patient DRPs in CKD patients.

    METHOD: An open-labeled, randomized control trial performed from April 2023 to July 2023 in the nephrology unit of a tertiary care setting in Peshawar Pakistan. Those patients who met the inclusion criteria were randomized into two groups 1:1, i.e., control and intervention group. Clinical pharmacists identified the DRPs at baseline using Pharmaceutical Care Network Europe (PCNE) 9.1 guidelines. The QoL of patients were assessed at baseline and endpoint by using the Functional Assessment of Non-Life-Threatening Conditions (FANLTC) questionnaire.

    RESULTS: A total of 100 patients were recruited having 50 in each group. The pharmacist identified a total of n = 230 DRPs in the intervention group, majority of the DRPs were attributed to inappropriate drug selection according to guidelines/formulary"; "inappropriate combinations of drugs or with herbal medications or dietary supplements"; and situations where "too many different drugs or active ingredients were prescribed". There was 46.52% reduction in the DRPs while comparing baseline and endpoint interventions suggested by pharmacist in the intervention group. The clinical pharmacist provided interventions in order to resolve the DRPs, and 37.40% interventions were accepted and fully implemented; 31.30% of the interventions were accepted and partially implemented. The clinical pharmacist identification and proposed intervention for DRPs contributed to a statistically significant improvement in QoL, from mean ±  SD scored 58.64 ±  9.10 at the baseline to 74.48 ± 10.11 at the endpoint, with a p-value of

    MeSH terms: Adult; Aged; Female; Humans; Male; Middle Aged; Pakistan; Pharmacists*; Quality of Life*; Surveys and Questionnaires
  7. Xiao Q, Wang C
    PLoS One, 2025;20(2):e0318070.
    PMID: 39899639 DOI: 10.1371/journal.pone.0318070
    Electrocardiogram (ECG) signals are crucial in diagnosing cardiovascular diseases (CVDs). While wavelet-based feature extraction has demonstrated effectiveness in deep learning (DL)-based ECG diagnosis, selecting the optimal wavelet base poses a significant challenge, as it directly influences feature quality and diagnostic accuracy. Traditional methods typically rely on fixed wavelet bases chosen heuristically or through trial-and-error, which can fail to cover the distinct characteristics of individual ECG signals, leading to suboptimal performance. To address this limitation, we propose a reinforcement learning-based wavelet base selection (RLWBS) framework that dynamically customizes the wavelet base for each ECG signal. In this framework, a reinforcement learning (RL) agent iteratively optimizes its wavelet base selection (WBS) strategy based on successive feedback of classification performance, aiming to achieve progressively optimized feature extraction. Experiments conducted on the clinically collected PTB-XL dataset for ECG abnormality classification show that the proposed RLWBS framework could obtain more detailed time-frequency representation of ECG signals, yielding enhanced diagnostic performance compared to traditional WBS approaches.
    MeSH terms: Algorithms; Cardiovascular Diseases/diagnosis; Humans; Signal Processing, Computer-Assisted; Wavelet Analysis*
  8. Sta Maria CC, Wu PH, Pratama Hasibuan D, Sinta Saragih C, Giap H, Nguyen DH, et al.
    ACS Appl Mater Interfaces, 2025 Feb 12;17(6):9514-9522.
    PMID: 39899891 DOI: 10.1021/acsami.4c17178
    Among Group III-A metal monochalcogenides, gallium telluride (GaTe) is one of the less studied materials in terms of applications and optical characterization. For the temperature dependence of the energy transitions in GaTe, photoluminescence (PL) spectroscopy is commonly used, and photomodulated reflectance (PR) is yet to be reported. In this work, layered monoclinic GaTe single crystals were synthesized by the Bridgman technique and used for the investigation of the conduction band (CB) edge and free-exciton (FX) state transitions using PR spectroscopy. Both energy transitions (i.e., absorption and emission) were present at room temperature at 1.656 and 1.647 eV for the CB edge transition (≡Eg) and for the FX state transition, respectively, and show a blueshift at cryogenic temperatures that can be fitted with Varshni's equation. The estimated E(0) is 1.794 eV for Eg and 1.776 eV for the FX transitions at 0 K. The energy of the FX state transition is ∼18 meV lower than that of the band gap (Eg) at 0 K. PL spectroscopy confirms that the PL emission is only the FX state transition that is lower than Eg. The temperature-induced band-gap shifting is related to performing temperature-dependent photodetector experiments using various incident light wavelengths. At 80 K, the responsivity of the single-crystal GaTe photodetector to the energies of wavelengths (735 and 845 nm) smaller than Eg is relatively smaller than that to 630 nm incident light. This indicates that the low-temperature band-gap shift plays a role in applications of GaTe in optoelectronics.
  9. Wei M, Yusuf A, Hsien CCM, Marzuki MA
    Int J Nurs Stud, 2025 Apr;164:104983.
    PMID: 39899940 DOI: 10.1016/j.ijnurstu.2024.104983
    BACKGROUND: Cancer is a life-threatening disease that can have a significant impact on patients' psychological well-being. Behavioural activation is an emerging psychological therapy that has been suggested effective in improving depression and anxiety. However, no review has yet summarised its effects on psychological distress among people with cancer.

    OBJECTIVE: To identify studies of behavioural activation designed for people with cancer and examine the effects on psychological distress, including depression and anxiety.

    DESIGN: Systematic review and meta-analysis.

    METHODS: A systematic search of PubMed/MEDLINE, CINAHL, EMBASE, PsycINFO, and the Cochrane Library was performed from the inception to 6 April 2024. Randomised controlled trials reporting on the effects of behavioural activation on psychological distress among cancer patients were included. Two authors independently screened the eligible studies, assessed the quality of studies, and extracted data. The risk of bias was assessed using version 2 of the Cochrane risk-of-bias tool for randomised trials (RoB 2). The meta-analysis was performed by Review Manager 5.4, and narrative synthesis was employed when the meta-analysis was inappropriate. The Grading of Recommendations, Assessment, Development and Evaluation (GRADE) system was used to assess the certainty of the evidence.

    RESULTS: A total of nine studies were included in this systematic review, with 1811 participants. The pooled analysis showed that behavioural activation could improve depression (SMD = -0.24, 95 % CI -0.44 - -0.03, p = 0.020; moderate quality of evidence), and anxiety (SMD = -0.56, 95 % CI -1.01 - -0.10, p = 0.020; low quality of evidence) among people with cancer. The effects were robust in sensitivity analysis and yielded consistent results in studies that were not pooled due to insufficient data. Subgroup analyses suggested that face-to-face and group administration were more effective, whereas the effects of different dosages were uncertain. Besides, the effects of behavioural activation at different follow-up periods were not identified There was no consensus on the optimal components of intervention.

    CONCLUSIONS: The evidence for behavioural activation as an effective treatment of psychological distress among people with cancer is promising. However, it should be noted that the quality of evidence was moderate and low, thus emphasising the need for caution when applying these findings. In order to explore which components may be most effective in improving psychological outcomes, more rigorous study designs and more detailed descriptions of interventions are necessary.

    REGISTRATION: The protocol was registered on PROSPERO (Registration number: CRD42024533171).

    MeSH terms: Behavior Therapy/methods; Humans; Stress, Psychological
  10. Al-Qaaneh AM, Abosaoda MK, Baldaniya L, Hamid JA, Sabarivani A, Panigrahi R, et al.
    Comput Biol Chem, 2025 Apr;115:108369.
    PMID: 39899941 DOI: 10.1016/j.compbiolchem.2025.108369
    The study employs density functional theory (DFT) to examine the drug-loading efficiency of graphyne (GYN) as a vehicle for the Tioguanine (TG) drug. The researchers analyzed the interaction energy, electrical properties of pure GYN, TG molecules, and TG@GYN complex to determine their effectiveness as a carrier. Configuration a, which utilized nitrogen and sulfur atoms in interactions, was deemed the most suitable among the three considered TG sites. Gas-phase interaction between TG drug and GYN resulted in an energy of adsorption about -1.64 eV. The study utilized non-covalent interaction (NCI) analysis to assess the interaction between GYN and TG drug, indicating weak forces of interaction in the TG@GYN complex. The HOMO-LUMO and charge-decomposition analysis described the transfer of charge from TG molecules to pure GYN during formation of TG@GYN. The results suggest that GYN could function as a promising candidate for carrying and delivering TG drug, leading to further research into similar 2D nanomaterials for drug transport applications.
    MeSH terms: Thioguanine/chemistry; Molecular Structure; Drug Delivery Systems
  11. Chye A, Jan S, Pathy NB, Luna HGC, Lim SB, Kimman M, et al.
    BMJ Glob Health, 2025 Feb 03;10(2).
    PMID: 39900428 DOI: 10.1136/bmjgh-2024-016402
    INTRODUCTION: Women in the Philippines experience significant health and economic burdens of breast cancer. The Philippines has reformed financial protection for breast cancer but does not have a national early detection and treatment programme. This study aims to model the health and economic impacts of ACT NOW (a pilot breast cancer programme that navigates women through free early detection to treatment) through an extended cost-effectiveness analysis.

    METHODS: A microsimulation decision tree model was used to model the ACT NOW intervention (including annual clinical breast examination (CBE) and biannual breast ultrasound for women at high risk of breast cancer) over 5 years for healthy women 40-69 years old. Outcomes included health gains (breast cancer deaths saved), financial protection (financial catastrophes saved) and incremental cost-effectiveness ratios (ICER) (cost per disability-adjusted life year (DALY) saved). Outcomes were stratified by income group. Probabilistic, one-way sensitivity and scenario analyses explored uncertainty.

    RESULTS: Over 5 years, the ACT NOW intervention is cost-effective with an ICER of PHP60 711 (USD1098) (average incremental cost PHP743 [95% UI 424-960] and DALYs saved 0.01 [95% UI 0.01-0.02], below Philippines 2022 gross domestic product per capita PHP178 751). Per 100 000 women, 57 deaths and eight financial catastrophes were saved. Cost-effectiveness did not vary significantly by income, but higher income groups incurred greater costs and lower DALYs. Results were sensitive to proportion of late-stage breast cancers post intervention, treatment adherence, intervention costs and downstaging effectiveness. Trade-offs are apparent between government contributions to financial protection and rates of financial catastrophe.

    CONCLUSIONS: Early detection interventions (annual CBE, biannual breast ultrasound if at high risk of breast cancer) are likely to be cost-effective, reduce breast cancer-related mortality through detection at earlier stages and modestly effective in reducing the incidence of financial catastrophe. Further research is required to establish the best implementation model to pursue full implementation and ways of designing equity-based screening interventions.

    MeSH terms: Adult; Aged; Cost-Benefit Analysis*; Female; Humans; Middle Aged; Philippines; Pilot Projects
  12. Al-Ajli FO, Formenti G, Fedrigo O, Tracey A, Sims Y, Howe K, et al.
    Sci Rep, 2025 Feb 04;15(1):4154.
    PMID: 39900672 DOI: 10.1038/s41598-025-88216-9
    The taxonomic classification of a falcon population found in the Mongolian Altai region in Asia has been heavily debated for two centuries and previous studies have been inconclusive, hindering a more informed conservation approach. Here, we generated a chromosome-level gyrfalcon reference genome using the Vertebrate Genomes Project (VGP) assembly pipeline. Using whole genome sequences of 49 falcons from different species and populations, including "Altai" falcons, we analyzed their population structure, admixture patterns, and demographic history. We find that the Altai falcons are genomic mosaics of saker and gyrfalcon ancestries, and carry distinct W and mitochondrial haplotypes that cluster with the lanner falcon. The Altai maternally-inherited haplotypes diverged 422,000 years before present (290,000-550,000 YBP) from the ancestor of sakers and gyrfalcons, both of which, in turn, split 109,000 YBP (70,000-150,000 YBP). The Altai W chromosome has 31 coding variants in 29 genes that may possibly influence important structural, behavioral, and reproductive traits. These findings provide insights into the question of Altai falcons as a candidate distinct species.
    MeSH terms: Animals; Chromosomes/genetics; Genetics, Population; Haplotypes*; Mongolia; Phylogeny; Genome; Genomics/methods
  13. Chen J, Jia J, Zhuang H, Zhang W, Yang JM, Tanwandee T, et al.
    Hepatol Int, 2025 Feb 03.
    PMID: 39900677 DOI: 10.1007/s12072-024-10744-9
    BACKGROUND: The Asia-Pacific (AP) region carries a substantial burden of HBV. Affordable HBV treatment is crucial to attain WHO's elimination goal. This study assesses the pricing and affordability of HBV treatment in AP.

    METHODS: A survey conducted among APASL members from 2 Aug to 30 Oct, 2023, gathered data on antiviral HBV drugs, treatment costs covering stages of chronic hepatitis B (CHB), compensated cirrhosis (CC), hepatocellular carcinoma (HCC), liver transplant, and monitoring expenses. Drug costs for TDF and ETV were compared to international reference price (TDF: $30, ETV: $36 per person per year), generating a median price ratio (MPR) where MPR 

  14. Salowi MA, Naing NN, Mustafa N, Nawang WRW, Sharudin SN, Husni MA
    BMC Public Health, 2025 Feb 03;25(1):435.
    PMID: 39901209 DOI: 10.1186/s12889-025-21602-0
    BACKGROUND: Effective Cataract Surgical Coverage (eCSC) is one of the Universal Health Coverage (UHC) indicators recommended by the World Health Organization (WHO). It is calculated from a population survey and measures access and quality of eye care services in the community. We conducted simultaneous population-based eye surveys in two regions in Malaysia in 2023 to estimate eCSC and compare the results with the survey in 2014 following the implementation of a mobile cataract program.

    METHODS: The surveys were simultaneously done in Eastern and Sarawak administrative regions using the Rapid Assessment of Avoidable Blindness (RAAB) technique. It involved a multistage cluster sampling method, each cluster comprising 50 residents aged 50 years and older. Presenting visual acuity (PVA) was checked, and subjects with cataracts were identified. The corrected VA (Pinhole) of those who had undergone cataract surgery was measured. eCSC was calculated at all levels of cataract surgical thresholds according to the protocol. The findings were compared with the previous survey.

    RESULTS: A total of 10,184 subjects were enumerated, with 9,709 examined and 475 non-respondents. Females had a significantly lower Cataract Surgical Coverage (CSC) than males for cataract surgical threshold of

    MeSH terms: Aged; Aged, 80 and over; Cataract; Female; Health Services Accessibility; Humans; Malaysia; Male; Middle Aged; Mobile Health Units; Visual Acuity; Program Evaluation; Universal Coverage
  15. Khan S, Noor S, Javed T, Naseem A, Aslam F, AlQahtani SA, et al.
    BioData Min, 2025 Feb 03;18(1):12.
    PMID: 39901279 DOI: 10.1186/s13040-024-00415-8
    Posttranslational modifications (PTMs) are essential for regulating protein localization and stability, significantly affecting gene expression, biological functions, and genome replication. Among these, sumoylation a PTM that attaches a chemical group to protein sequences-plays a critical role in protein function. Identifying sumoylation sites is particularly important due to their links to Parkinson's and Alzheimer's. This study introduces XGBoost-Sumo, a robust model to predict sumoylation sites by integrating protein structure and sequence data. The model utilizes a transformer-based attention mechanism to encode peptides and extract evolutionary features through the PsePSSM-DWT approach. By fusing word embeddings with evolutionary descriptors, it applies the SHapley Additive exPlanations (SHAP) algorithm for optimal feature selection and uses eXtreme Gradient Boosting (XGBoost) for classification. XGBoost-Sumo achieved an impressive accuracy of 99.68% on benchmark datasets using 10-fold cross-validation and 96.08% on independent samples. This marks a significant improvement, outperforming existing models by 10.31% on training data and 2.74% on independent tests. The model's reliability and high performance make it a valuable resource for researchers, with strong potential for applications in pharmaceutical development.
  16. Alnsour ZR, Alnsour MR, Alnsour AR, Massadeh MM, Alzoubi KH, Masadeh MM
    PMID: 39901542 DOI: 10.2174/0127724328328331241217194034
    Examining antifungal resistance in dermatophytes is crucial in infectious diseases, dermatology, and clinical microbiology. The increasing occurrence of resistant infections and their influence on the effectiveness of therapy seem overwhelming. This study examines the present condition of antifungal resistance in dermatophytes, highlighting the need for ongoing and up-to-date research. Fungal diseases constantly change, and fungi have developed new resistance mechanisms. Here, we analyze the historical context of research on antifungal resistance, examining the variables that contribute to the development of resistance, such as the growing use of antifungals in clinical and agricultural contexts. We also explore the consequences of resistance to antifungal agents in clinical practice and public health. The review emphasizes the significance of new diagnostic technologies, like next-generation sequencing, in comprehending resistance mechanisms. It also underscores the crucial role of international collaboration in tackling this worldwide health concern. In conclusion, the paper emphasizes the need for continuous research to adjust to the evolving epidemiology of dermatophyte infections, create efficient treatment approaches, and guide public health interventions. This will ensure that the management of antifungal resistance is grounded in the most up-to-date scientific knowledge and optimal methods.
  17. Verma S, Malviya R, Sundaram S, Srivastava S, Sridhar SB, Rab SO, et al.
    Curr Top Med Chem, 2025 Jan 30.
    PMID: 39901550 DOI: 10.2174/0115680266327892241223052456
    Statins are a class of hypolipidemic agents that have been shown to promote osteogenic differentiation through enhanced alveolar bone recovery, inserted osseointegration, and cartilage regeneration. This review uses Molecular Docking (MD) simulations and additional Computer- Aided Drug Design (CADD) methods to present the state of the art in statin therapy. Furthermore, several studies have shown that factors such as limited overall absorption, metabolism in the first pass, and systemic side effects are among those that affect the oral administration of statins. In addition, these variables include susceptibility to efflux mechanisms, drug permeability, dissolution percentage, aqueous solubility, initial metabolism, and pre-systemic metabolism. Additionally examined are the pharmacokinetics of the statin and in vivo mechanisms of action. As a result of the numerous problems associated with the consumption of statins, including their low total bioavailability, first-pass metabolism, low aqueous solubility, and systemic adverse reactions, a non-oral mode of administration was looked into for this crucial and primary class of pharmacokinetic agents. However, to optimize bioavailability and minimize side effects, more research is required.
  18. Chen J, Lou Y, Sun L, Chia CH, Nilghaz A, Tian J
    ACS Sens, 2025 Feb 28;10(2):1356-1365.
    PMID: 39901574 DOI: 10.1021/acssensors.4c03277
    Maximizing the efficiency of electrode usage is a crucial step in enhancing the integration of wearables. Currently, electrodes are combined in an additive manner to enable multiplexed sweat screening. The additive sensor requires significant space to accommodate single-function electrodes, which limits the integration of the wearable sensors. Here, we report that the versatility of a single electrode is achieved by assigning different roles to the electrode at different times, resulting in a flexible, disposable, epidermal sweat-sensing platform that integrates in situ iontophoresis and three electrochemical sensors on only four electrodes, while previous platforms required at least seven electrodes. For example, the iontophoresis electrode serves as the working electrode (WE) for chloride sensing and as the counter electrode (CE) for pH sensing after its controllable release of pilocarpine, and the sulfonated polyaniline (SPAN) modified glucose oxidase (GOx) serves as the WE for both pH and glucose sensing. All four functions are integrated into an 8 mm2 (1.8 × 4.45 mm) sensing area, requiring a sample volume of approximately 1 μL. These results open possibility for highly integrated wearable sweat sensors and multimodal sensors.
    MeSH terms: Aniline Compounds/chemistry; Chlorides/analysis; Chlorides/chemistry; Electrodes*; Enzymes, Immobilized/metabolism; Enzymes, Immobilized/chemistry; Glucose/analysis; Humans; Hydrogen-Ion Concentration; Iontophoresis/instrumentation; Iontophoresis/methods; Pilocarpine; Biosensing Techniques/instrumentation; Biosensing Techniques/methods; Electrochemical Techniques/instrumentation; Electrochemical Techniques/methods
  19. Ma G, Eng AE, Chiang SK, Hao F, McIntyre RS, Zhou D, et al.
    Psychiatry Clin Neurosci, 2025 Apr;79(4):165-175.
    PMID: 39901842 DOI: 10.1111/pcn.13788
    AIM: Attention deficit hyperactivity disorder (ADHD) is a neurodevelopmental disorder that may persist into adulthood, with no established objective diagnostic tool yet. This study aims to propose a multimodal objective assessment tool involving clinical assessments, functional neuroimaging, and oculomotricity measurement for ADHD in young adults.

    METHODS: Seventy-one medication-naïve patients and 71 healthy controls (HCs) aged 18 to 28 underwent clinical interviews, Conners' Adult ADHD Rating Scale (CAARS) questionnaire, functional near-infrared spectroscopy (fNIRS), oculomotricity task, and Conners' Continuous Performance Task (CPT) 3rd edition. Student's t-tests with Bonferroni's correction were performed to compare the performance between groups, and logistic regression was used for classification.

    RESULTS: ADHD patients had significantly lower frontal hemodynamic response during verbal fluency task (VFT) (P = 0.0003), more anticipatory eye movements during overlap task (P = 0.0006), higher latency (P 

    MeSH terms: Adolescent; Adult; Female; Humans; Male; Neuropsychological Tests/standards; Psychomotor Performance/physiology; Case-Control Studies; Young Adult; Functional Neuroimaging*
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