Browse publications by year: 2025

  1. Paradiso C, Gratton P, Trucchi E, López-Delgado J, Gargano M, Garizio L, et al.
    Mol Phylogenet Evol, 2025 Mar;204:108294.
    PMID: 39880223 DOI: 10.1016/j.ympev.2025.108294
    Galápagos iguanas are a monophyletic group endemic to the Galápagos archipelago, comprising the marine iguana Amblyrhynchus cristatus and three species of land iguanas: Conolophus subcristatus, C. pallidus and C. marthae. The biogeographic history of the land species in relation to their current distributions remains uncertain, in particular the origins of C. marthae, which is restricted to a small area of the northern part of Isabela Island. The classification of C. pallidus as a separate species has also been debated. We analyzed DNA sequences (RADseq) to reconstruct demographic histories of selected local populations of all Galápagos iguana species and estimate their divergence times within a multispecies coalescent framework. Our results indicate an early date for the colonization of Galápagos by iguanas, relative to island formation, at ca. 10 Mya, and support a recent split of C. marthae via allopatric speciation, after the emergence of Isabela Island, at ca. 0.57 Mya. We find contrasting demographic histories in C. marthae and the syntopic population of C. subcristatus, suggesting competitive interaction between these species. We also confirm that the divergence of C. pallidus from C. subcristatus is recent (0.09 Mya) and close in time to the split between populations of C. subcristatus from different islands. Our genetic data support recent census estimates indicating a relatively small current effective population size (Ne) in all the studied populations. Our findings shed light on the evolutionary history of Galápagos iguanas and emphasize the need for targeted conservation strategies.
    MeSH terms: Animals; Bayes Theorem; Ecuador; Genetics, Population; Models, Genetic; Phylogeny*; Sequence Analysis, DNA; Evolution, Molecular; Genomics; Genetic Speciation; Phylogeography*
  2. Jeys LM, Morris GV, Kurisunkal VJ, Botello E, Boyle RA, Ebeid W, et al.
    Bone Joint J, 2025 Feb 01;107-B(2):246-252.
    PMID: 39889743 DOI: 10.1302/0301-620X.107B2.BJJ-2024-0643.R1
    AIMS: The Birmingham Orthopaedic Oncology Meeting (BOOM), held in January 2024, convened 309 delegates from 53 countries to discuss and refine 21 consensus statements on the optimal management of chondrosarcoma.

    METHODS: With representation from Europe (43%; n = 133), North America (17%; n = 53), South America (16%; n = 49), Asia (13%; n = 40), Australasia (5%; n = 16), the Middle East (4%; n = 12), and Africa (2%; n = 6), the combined experience of treating bone sarcomas among attendees totalled approximately 30,000 cases annually, equivalent to 66 years of experience in the UK alone. The meeting's process began with the formation of a local organizing committee, regional leads, and a scientific committee comprising representatives from 150 specialist units across 47 countries. Supported by major orthopaedic oncology organizations, the meeting used a modified Delphi process to develop consensus statements through online questionnaires, thematic groupings, narrative reviews, and anonymous pre-meeting polling.

    RESULTS: Strong (> 80%) consensus was achieved on 19 out of 21 statements, reflecting agreement among delegates. Key areas of consensus included the role of radiology in diagnosis and surveillance, the management of locally recurrent disease, and the treatment of dedifferentiated chondrosarcoma. Notably, there was agreement that routine chemotherapy has no role in chondrosarcoma treatment, and radiological surveillance is safe for intraosseous chondrosarcomas. Despite the overall consensus, areas of controversy remain, particularly regarding the treatment of atypical cartilage tumours and surgical margins. These unresolved issues underscore the need for further research and collaboration within the orthopaedic oncology community.

    CONCLUSION: BOOM represents the largest global consensus meeting in orthopaedic oncology, providing valuable guidance for clinicians managing chondrosarcoma worldwide. The consensus statements offer a reference for clinical practice, highlight key research priorities, and aim to improve patient outcomes on a global scale.

    MeSH terms: Delphi Technique; Humans; Orthopedics; Consensus; Biomedical Research
  3. Abdul Rahman N, Jose Jol C, Albania Linus A, Taib SNL, Parabi A, Kwong Ming C, et al.
    Environ Res, 2025 Jan 29;270:121005.
    PMID: 39889876 DOI: 10.1016/j.envres.2025.121005
    The treatment of brackish peat water presents a formidable challenge due to its elevated levels of natural organic matter and salinity which not only hinder conventional water treatment systems but also necessitate an innovative approach to effectively manage these complex water characteristics. In response to these challenges, electrocoagulation has emerged as a promising alternative by utilizing electrochemical processes to efficiently destabilize and eliminate contaminants in brackish peat water sources. As such, this review aims to unveil challenges of aluminium electrodes fouling and passivation in electrocoagulation treatment system for sustainable water management of coastal Borneo peatlands. Several studies in the literature highlight that key operating parameters, especially electric current and voltage which play a pivotal role in influencing the overall effectiveness of these electrocoagulation systems. Although aluminium electrodes demonstrate high contaminants removal efficiencies, it remains susceptible to fouling and passivation due to contaminant buildup and oxide layer formation which increase electrical resistance and decrease electroactivity of redox reactions. The novelty of this review lies in its focused synthesis of fouling and passivation dynamics through the integration of Tafel plot analyses and advanced characterization techniques, particularly Energy Dispersive X-Ray (EDX) spectroscopy. Furthermore, a thorough understanding of the adsorption mechanisms, particularly through the interaction between aluminium hydroxides and contaminants is essential for enhancing system efficiency and mitigating fouling. Additionally, optimizing the electrocoagulation treatment system and conducting a detailed analysis of adsorption mechanisms, particularly through Tafel plot analysis are pivotal for enhancing the system efficiency. Advanced analytical methods such as Energy Dispersive X-Ray (EDX) spectroscopy provide deeper insights into floc composition that essential for improving contaminants removal strategies. Overall, this review offers a focused assessment on the interplay between brackish peat water and electrocoagulation in order to provide a foundation for future research aimed at developing sustainable treatment systems for coastal Borneo peatlands.
  4. Abdul Aziz AF, Ong T
    BMC Public Health, 2025 Jan 31;25(1):398.
    PMID: 39891072 DOI: 10.1186/s12889-025-21358-7
    BACKGROUND: The average person spends 1/3 of their lives working. However, approximately 15% of working adults worldwide are struggling with a mental disorder at any given time. The COVID-19 pandemic has spearheaded the importance of employee mental health, highlighting the role that employers can play in preserving employee wellbeing. Although Employee Assistance Programs (EAPs) are slowly becoming an established practice, it is still a relatively new phenomenon in most of Asia. This study therefore aims to investigate the prevalence of employees in the Southeast Asian region who have access to EAPs and understand the relationship between EAP access and employee wellbeing.

    METHODS: This cross-sectional, online survey-based study involved 15,302 employees from Malaysia, Singapore, Philippines, Thailand, Indonesia, and Vietnam who completed the Depression, Anxiety, Stress Scales (DASS-21), Thriving from Work Questionnaire (TfWQ), and sociodemographic measures. Employee productivity and turnover intention was also measured as a secondary outcome. Hierarchical regression models were conducted to determine the influence of EAP access on employee wellbeing. Subgroup analyses were conducted on employees with access to EAPs to explore how different forms of EAP services contribute to employee wellbeing.

    RESULTS: Only 29.04% of employees in the region reported being aware of having access to any forms of EAP services provided by their employers. After controlling for sociodemographic variables, access to EAP was significantly associated with improved mental health, thriving, and productivity, and reduced turnover intention (p 

    MeSH terms: Adult; Asia, Southeastern; Cross-Sectional Studies; Female; Health Services Accessibility/statistics & numerical data; Humans; Male; Mental Health/statistics & numerical data; Middle Aged; Occupational Health Services/statistics & numerical data; Personnel Turnover/statistics & numerical data; Surveys and Questionnaires; Occupational Health; Young Adult
  5. Khojasteh L, Kafipour R, Pakdel F, Mukundan J
    BMC Med Educ, 2025 Jan 31;25(1):159.
    PMID: 39891148 DOI: 10.1186/s12909-025-06753-3
    BACKGROUND AND OBJECTIVES: Assessing and improving academic writing skills is a crucial component of higher education. To support students in this endeavor, a comprehensive self-assessment toolkit was developed to provide personalized feedback and guide their writing improvement. The current study aimed to rigorously evaluate the validity and reliability of this academic writing self-assessment toolkit.

    METHODS: The development and validation of the academic writing self-assessment toolkit involved several key steps. First, a thorough review of the literature was conducted to identify the essential criteria for authentic assessment. Next, an analysis of medical students' reflection papers was undertaken to gain insights into their experiences using AI-powered tools for writing feedback. Based on these initial steps, a preliminary version of the self-assessment toolkit was devised. An expert focus group discussion was then convened to refine the questions and content of the toolkit. To assess content validity, the toolkit was evaluated by a panel of 22 medical student participants. They were asked to review each item and provide feedback on the relevance and comprehensiveness of the toolkit for evaluating academic writing skills. Face validity was also examined, with the students assessing the clarity, wording, and appropriateness of the toolkit items.

    RESULTS: The content validity evaluation revealed that 95% of the toolkit items were rated as highly relevant, and 88% were deemed comprehensive in assessing key aspects of academic writing. Minor wording changes were suggested by the students to enhance clarity and interpretability. The face validity assessment found that 92% of the items were rated as unambiguous, with 90% considered appropriate and relevant for self-assessment. Feedback from the students led to the refinement of a few items to improve their clarity in the context of the Persian language. The robust reliability testing demonstrated the consistency and stability of the academic writing self-assessment toolkit in measuring students' writing skills over time.

    CONCLUSION: The comprehensive evaluation process has established the academic writing self-assessment toolkit as a robust and credible instrument for supporting students' writing improvement. The toolkit's strong psychometric properties and user-centered design make it a valuable resource for enhancing academic writing skills in higher education.

    MeSH terms: Education, Medical, Undergraduate/methods; Educational Measurement/methods; Humans; Pilot Projects; Self-Assessment*; Students, Medical*; Writing*; Reproducibility of Results; Focus Groups
  6. Kistler BM, Biruete A, Wong MMY, Wang AY, Martin-Del-Campo F, Nerbass FB, et al.
    Nutr Clin Pract, 2025 Apr;40(2):307-318.
    PMID: 39891426 DOI: 10.1002/ncp.11271
    Protein-energy wasting is common in people with chronic kidney disease (CKD), especially in those undergoing kidney replacement therapy. Oral nutrition supplements and enteral nutrition are strategies that have been shown to improve nutrition status, and potentially outcomes. However, access to specialized commercial products for people with CKD is limited by factors including cost and regional availability. Homemade formulas represent a potentially cheaper, accessible, and more flexible option than commercial products, but they come with their own unique set of challenges. Furthermore, some aspects of homemade products, including consistency of nutrients, physical properties, and food safety, may pose challenges in the context of physiological changes that occur in CKD. Despite evidence of their use in CKD clinics, there have been few studies using homemade formulas in this population. This narrative review article summarizes the available literature on the potential usage, benefits, and concerns related to homemade formulas, emphasizing the unique challenges in people with CKD. Given the potential usage and limited research on homemade formulas in people with CKD, additional education and research are warranted to optimize the use of these tools in this clinical population.
    MeSH terms: Enteral Nutrition/methods; Food, Formulated*; Humans; Nutritional Status; Protein-Energy Malnutrition/etiology; Protein-Energy Malnutrition/prevention & control; Protein-Energy Malnutrition/therapy; Renal Replacement Therapy/methods; Nutritional Support/methods; Dietary Supplements
  7. Ramin NM, Salleh WMNHW, Salihu AS, Ab Ghani N, Agosto NJ, Sungthong B, et al.
    Nat Prod Res, 2025 Feb 01.
    PMID: 39891598 DOI: 10.1080/14786419.2025.2457117
    Piperaceae family includes numerous species used for food, spices, and medicinal purposes. In this study, the chemical composition and bioactivities (antioxidant, anticholinesterase, and anti-inflammatory activities) of the essential oil from Piper rostratum Roxb. were investigated. The essential oil was extracted via hydrodistillation and thoroughly analysed using gas chromatography-flame ionisation detection (GC-FID) and gas chromatography-mass spectrometry (GC-MS). A total of 20 chemical components were identified, accounting for 94.2% of the total oil. The major components were γ-muurolene (14.1%), δ-cadinene (13.2%), allylpyrocatechol diacetate (11.5%), chavicol (8.2%), α-humulene (7.8%), and hydroxychavicol (6.9%). The antioxidant activity was evaluated using the DPPH free radical scavenging assay, the acetylcholinesterase inhibitory activity was measured using the Ellman method, and the anti-inflammatory activity was assessed through lipoxygenase enzyme inhibition. The essential oil demonstrated moderate activity in DPPH free radical scavenging (IC50 = 98.5 µg/mL), acetylcholinesterase inhibition (IC50 = 89.2 µg/mL), and lipoxygenase inhibition (IC50 = 77.2 µg/mL).
  8. William A, Lachat C, Ahles S, Murphy KJ, Minihane AM, Weaver C, et al.
    Eur J Nutr, 2025 Feb 01;64(2):76.
    PMID: 39891676 DOI: 10.1007/s00394-024-03561-1
    PURPOSE: Inadequate reporting of nutrition data can hinder the success of nutrition health policies. CONSORT provides guidance for reporting of randomised controlled trials (RCTs) and is required by most journals today, yet reporting of nutrition interventions may benefit from a more tailored approach. A Federation of European Nutrition Societies working group was created to improve quality and completeness of reporting of nutrition trials, and our work to date features a proposal for a CONSORT extension specific to nutrition RCTs. The present manuscript describes a Delphi survey conducted to gather opinion from a wider panel of nutrition and health experts and related interest-holders on our proposal.

    METHODS: We invited 138 potentially eligible participants to take part in the Delphi survey from a representative spread of expertise and geography. We employed a Likert scale with comments for our 32-item proposal in round 1, and a dichotomous scale with comments for our 29-item proposal in round 2. Threshold for agreement was set at ≥ 80% for both rounds.

    RESULTS: Forty-seven potentially eligible participants responded to our invitation, 38 completed the first round and 36 completed the second. N = 23 (72%) items achieved ≥ 80% in round 1, and 100% of items in round 2. Three items were dropped or merged following round 1. A third Delphi round was not required to obtain consensus.

    CONCLUSIONS: This Delphi expert consensus proposes a 29-item checklist specific to the reporting of nutrition RCTs and will inform further development of guidance through forthcoming consensus meetings.

    MeSH terms: Delphi Technique*; Humans; Surveys and Questionnaires; Research Design/standards; Nutrition Policy; Consensus*; Nutritional Sciences/standards; Checklist
  9. Luo JX, Gao XT, Rong Z, Zhang LH, Sun YF, Qi ZL, et al.
    Mar Biotechnol (NY), 2025 Feb 01;27(1):41.
    PMID: 39891779 DOI: 10.1007/s10126-025-10420-5
    Mandarin fish (Siniperca chuatsi) is a voracious carnivorous species, usually consuming only live bait fish, but dietary acclimation enables it to accept artificial feed. However, the effects of dietary acclimation on intestinal performance and gene expression in mandarin fish and related mechanisms remain largely unknown. Therefore, this study investigated the effects of artificial feed on intestinal physicochemical and biochemical performance and gene expression in mandarin fish. Mandarin fish were sampled on day 10 after feeding with live dace (LD), at day 40 after subsequent feeding with dead dace plus artificial feed (DD + AF) from day 11 to day 40, and at day 90 after continuous feeding with artificial feed (AF) alone from day 41 to day 90 for transcriptome sequencing. The biochemical analysis results indicated that artificial feed significantly increased the activity of antioxidant enzymes glutathione peroxidase and superoxide dismutase in the intestine, liver, and stomach. Histological analysis demonstrated intestinal damage in mandarin fish fed with artificial feed. The GO and KEGG enrichment analyses indicated that the DEGs in AF vs. DD + AF were significantly enriched in the pentose phosphate pathway, and the DEGs in AF vs. LD were mainly significantly enriched in glycolysis/gluconeogenesis and PPAR signaling pathways. Nineteen feed acclimation-related key genes such as gene pfkfb4a and scd were identified in the intestine and found to exhibit upregulated expressions. These results revealed that artificial feed domestication enhanced the antioxidant capacity of the mandarin fish intestine and reduced hepatic lipid deposition by upregulating the related gene expression of mandarin fish and that the regulation of carbon metabolisms, including sugar, lipid, and steroid metabolisms, might be fundamental mechanisms for mandarin fish to acclimatize to dietary changes. These findings provide novel insights into the feed acclimation mechanism of mandarin fish, holding implications for promoting large-scale artificial feed aquaculture of mandarin fish and improving economic efficiency.
    MeSH terms: Animals; Animal Feed*; Diet/veterinary; Intestines*; Perciformes/genetics; Perciformes/metabolism; Aquaculture; Gene Expression Profiling; Transcriptome*; Domestication
  10. Shi Y, Deng C, Lu X, Wang Y, Pan Y, Su D, et al.
    Cell Rep, 2025 Feb 25;44(2):115258.
    PMID: 39891905 DOI: 10.1016/j.celrep.2025.115258
    Cuticles protect plants from water loss and pathogen attack. We address here the functional significance of SlGRAS9, SlZHD17, and SlMBP3 in regulating cutin formation in tomato fruit. The study unveils the role of the multipartite "SlGRAS9-SlZHD17-SlMBP3-SlMIXTA-like" transcription factor module in cutin biosynthesis. Plants deficient in SlGRAS9, SlZHD17, or SlMBP3 exhibit thickened cuticles and a higher accumulation of cutin monomers, conferring extended fruit shelf life and higher tolerance to postharvest fungal infection. SlGRAS9 regulation of cutin is mediated by SlZHD17, a negative regulator of SlCYP86A69. SlZHD17 acts synergistically with SlMBP3 to repress SlCYP86A69, and its interaction with SlMIXTA-like prevents the binding to the SlCYP86A69 promoter, thereby releasing the repression of cutin biosynthesis. SlZHD17 and SlMBP3 synergistically repress cutin biosynthesis, while SlMIXTA-like and SlCD2 act antagonistically to SlZHD17 and SlMBP3 on this metabolic pathway. The study defines targets for breeding strategies aimed at improving cuticle-associated traits in tomato and potentially other crops.
    MeSH terms: Fruit/genetics; Fruit/metabolism; Promoter Regions, Genetic/genetics; Gene Expression Regulation, Plant*
  11. Zou S, Zhang Z, Lee YY, Ai H, Zhang Y, Wang Y
    Food Chem, 2025 May 15;474:143151.
    PMID: 39891972 DOI: 10.1016/j.foodchem.2025.143151
    Acylglycerols are widely used in the food industry due to their antimicrobial, emulsifying and nutritional properties. This study investigated the key reaction parameters, mass transfer mechanisms, and kinetic features of lipase-catalyzed esterification in a solvent-free system. Taguchi method was further employed to explore the relationship between "reaction parameter - yield composition". The results revealed that the maximum selectivity were achieved fror MAG (57.72 %), DAG (82.67 %) and TAG (79.29 %) under different enzymatic conditions. Fatty acid-to-glycerol molar ratio had the greatest impact on DAG selectivity, contributing 38.08 % of total impact level. Mass transfer analysis showed that external mass transfer limitation could be effectively overcome at stirring speeds above 600 rpm. Kinetic analysis confirmed that the reaction followed the Ping-Pong BiBi mechanism with alcohol and acid inhibition (SSE = 0.000643). This work provided a theoretical basis for developing more efficient and selective catalytic processes, aiding in quality control, reactor design, and industrial scale-up.
    MeSH terms: Enzymes, Immobilized/metabolism; Enzymes, Immobilized/chemistry; Esterification; Fungal Proteins/metabolism; Fungal Proteins/chemistry; Glycerides/chemistry; Kinetics; Biocatalysis*
  12. Meteku BT, De Silva K, Green SE, Turner T
    J Clin Epidemiol, 2025 Jan 30;180:111707.
    PMID: 39892522 DOI: 10.1016/j.jclinepi.2025.111707
    OBJECTIVES: Evidence-based clinical guidelines have the potential to improve health care and health outcomes. Living guidelines methods provide an approach to ensuring guidelines are always up-to-date, maximizing this potential. However, to date, most work on living guidelines has been conducted in high income countries. The objective of this study is to explore the barriers and facilitators to the development, adaptation, and use of living guidelines among evidence-based guideline developers in low- and middle-income countries (LMICs).

    STUDY DESIGN AND SETTING: We used a descriptive qualitative study design. We employed purposive and snowball sampling techniques to recruit guideline developers from LMICs and World Health Organization offices to participate in online, semistructured interviews. Data were analysed using a thematic approach with NVivo 20 software. Ethics approval was granted by Monash University.

    RESULTS: We interviewed 18 participants from LMICs (Colombia, India, Iran, Indonesia, Argentina, and Malaysia) and WHO offices (including headquarters, regional offices, and country offices). Two main themes emerged, along with six associated subthemes. The main themes were as follows: (1) People in LMICs want living guidelines and (2) Resource limitations and their implications for living guidelines in LMICs.

    CONCLUSION: Our research identified that guideline developers in LMICs have a strong desire to both develop and use living guidelines, but are currently limited by important barriers. Initiatives to support development, adaptation, and use of living guidelines in LMICs may help overcome barriers and meet the need for living guidelines in LMICs. It is also essential to design strategies that overcome identified barriers to developing, adapting, and implementing living guidelines, such as a lack of resources, delays in updates, and limited accessibility.

    PLAIN LANGUAGE SUMMARY: This study looked at how living (continuously updated) approaches can be used to develop, adapt, and use clinical guidelines in settings with limited resources, and explored the pros and cons of each. The findings revealed a strong need to develop and use living guidelines in low-resource settings despite challenges, such as resource scarcity, delays in updating, and limited access to these guidelines. Overall, the findings revealed that living guidelines were worthwhile in LMICs despite all of the related challenges.

  13. Thuya WL, Cao Y, Ho PC, Wong AL, Wang L, Zhou J, et al.
    PMID: 39893129 DOI: 10.1016/j.cytogfr.2025.01.003
    The IL-6/JAK/STAT3 signaling pathway is a key regulator of tumor progression, immune evasion, and therapy resistance in various cancers. Frequently dysregulated in malignancies, this pathway drives cancer cell growth, survival, angiogenesis, and metastasis by altering the tumor microenvironment (TME). IL-6 activates JAK kinases and STAT3 through its receptor complex, leading to the transcription of oncogenic genes and fostering an immunosuppressive TME. This environment recruits tumor-associated macrophages (TAMs), cancer-associated fibroblasts (CAFs), and regulatory T cells (Tregs), collectively supporting immune evasion and tumor growth. IL-6/JAK/STAT3 axis also contributes to metabolic reprogramming, such as enhanced glycolysis and glutathione metabolism, helping cancer cells adapt to environmental stresses. Therapeutic targeting of this pathway has gained significant interest. Strategies include monoclonal antibodies against IL-6 or its receptor (e.g., Tocilizumab, Siltuximab), JAK inhibitors (e.g., Ruxolitinib), and STAT3-specific inhibitors (e.g., Napabucasin), which have exhibited promise in preclinical and initial clinical studies. These inhibitors can suppress tumor growth, reverse immune suppression, and enhance the efficacy of immunotherapies like immune checkpoint inhibitors. Combination therapies that integrate IL-6 pathway inhibitors with conventional treatments are particularly promising, addressing resistance mechanisms and improving patient outcomes. Advances in biomarker-driven patient selection, RNA-based therapies, and isoform-specific inhibitors pave the way for more precise interventions. This review delves into the diverse roles of IL-6/JAK/STAT3 signaling in cancer progression, therapeutic strategies targeting this pathway, and the potential for integrating these approaches into personalized medicine to enhance treatment outcomes.
  14. Kow CS, Ramachandram DS, Hasan SS, Thiruchelvam K
    J Mycol Med, 2025 Mar;35(1):101534.
    PMID: 39892065 DOI: 10.1016/j.mycmed.2025.101534
    INTRODUCTION: Vulvovaginal candidiasis (VVC) is a prevalent fungal infection affecting millions of women globally, primarily caused by Candida species, most notably Candida albicans. Ibrexafungerp emerges as a promising candidate in the treatment arsenal against VVC, presenting a novel approach to combating this prevalent fungal infection.

    METHODS: A systematic literature search was conducted across major databases, including PubMed, EMBASE, and the Cochrane Library, to identify relevant randomized controlled trials (RCTs) evaluating the efficacy and safety of ibrexafungerp in the treatment of VVC. Following rigorous methodology, data extraction, risk of bias assessment using Cochrane's RoB 2 tool, and meta-analysis were conducted.

    RESULTS: Four RCTs were included in the analyses. The ibrexafungerp regimen utilized across the studies were 300 mg administered twice daily for one day. Meta-analysis revealed that ibrexafungerp was associated with significantly higher clinical cure rates compared to placebo in patients with VVC (pooled odds ratio (OR) 2.32; 95 % confidence interval (CI) 1.80 to 2.98). Complete symptom resolution was achieved in a greater proportion of participants receiving ibrexafungerp (pooled OR 2.76; 95 % CI 1.62 to 4.71). Analysis of treatment-emergent adverse events revealed a significant higher incidence of at least one treatment-emergent adverse event with ibrexafungerp compared to placebo (pooled OR 2.83; 95 % CI 2.06 to 3.88).

    CONCLUSION: This study provides robust support for the efficacy of ibrexafungerp in the treatment of VVC. While the safety profile of ibrexafungerp appears favorable with mostly mild adverse events reported, decision-making in the clinical context should be guided by individual patient factors.

    MeSH terms: Candida albicans/drug effects; Female; Glycosides/administration & dosage; Glycosides/adverse effects; Glycosides/therapeutic use; Humans; Triterpenes/administration & dosage; Triterpenes/adverse effects; Triterpenes/therapeutic use; Randomized Controlled Trials as Topic*; Treatment Outcome
  15. Hassan BAR, Mohammed AH
    J Infect Chemother, 2025 Apr;31(4):102638.
    PMID: 39892512 DOI: 10.1016/j.jiac.2025.102638
    INTRODUCTION: Bacterial resistance poses a significant threat to cancer patients due to their compromised immune systems and frequent use of antibiotics. Understanding oncologists' knowledge of this issue is essential for improving patient care. This study aimed to develop and validate a questionnaire assessing oncologists' knowledge of the relationship between bacterial resistance and cancer.

    METHODS: A cross-sectional study involving 421 participants was conducted in Iraq from November 2023 to March 2024 across various cancer centers and oncology hospitals. A 41-item questionnaire covering eight domains-Risk Factors, Infections and Antibiotic Use, Mechanisms of Resistance, Impact on Drug Efficacy and Toxicity, Genetic Factors, Microbial Adaptation, Impact of Gut Microbiota, and Antimicrobial Stewardship-was developed. Content validity was evaluated by five experts. Exploratory Factor Analysis (EFA) and Confirmatory Factor Analysis (CFA) were used to assess construct validity, while Item Response Theory (IRT) evaluated item performance. Reliability was measured using Cronbach's Alpha and Composite Reliability (CR).

    KEY FINDINGS: The questionnaire showed strong content validity, with most items achieving a Content Validity Index (CVI) of ≥0.8. EFA identified eight factors, confirmed by CFA with acceptable model fit (CFI = 0.96, TLI = 0.95, RMSEA = 0.05). IRT indicated strong item discrimination and acceptable difficulty. Cronbach's Alpha was 0.88, with CR at 0.89.

    CONCLUSION: This validated 41-item questionnaire provides a reliable tool for assessing oncologists' knowledge of bacterial resistance among cancer patients. It helps identify knowledge gaps and guide interventions to improve oncology practice outcomes.

    MeSH terms: Adult; Anti-Bacterial Agents/therapeutic use; Cross-Sectional Studies; Factor Analysis, Statistical; Female; Humans; Iraq; Health Knowledge, Attitudes, Practice*; Male; Middle Aged; Models, Theoretical; Risk Factors; Reproducibility of Results; Drug Resistance, Bacterial*
  16. Brodie JF, Bello C, Emer C, Galetti M, Luskin MS, Osuri A, et al.
    Conserv Biol, 2025 Feb;39(1):e14414.
    PMID: 39466005 DOI: 10.1111/cobi.14414
    The urgent need to mitigate and adapt to climate change necessitates a comprehensive understanding of carbon cycling dynamics. Traditionally, global carbon cycle models have focused on vegetation, but recent research suggests that animals can play a significant role in carbon dynamics under some circumstances, potentially enhancing the effectiveness of nature-based solutions to mitigate climate change. However, links between animals, plants, and carbon remain unclear. We explored the complex interactions between defaunation and ecosystem carbon in Earth's most biodiverse and carbon-rich biome, tropical rainforests. Defaunation can change patterns of seed dispersal, granivory, and herbivory in ways that alter tree species composition and, therefore, forest carbon above- and belowground. Most studies we reviewed show that defaunation reduces carbon storage 0-26% in the Neo- and Afrotropics, primarily via population declines in large-seeded, animal-dispersed trees. However, Asian forests are not predicted to experience changes because their high-carbon trees are wind dispersed. Extrapolating these local effects to entire ecosystems implies losses of ∼1.6 Pg CO2 equivalent across the Brazilian Atlantic Forest and 4-9.2 Pg across the Amazon over 100 years and of ∼14.7-26.3 Pg across the Congo basin over 250 years. In addition to being hard to quantify with precision, the effects of defaunation on ecosystem carbon are highly context dependent; outcomes varied based on the balance between antagonist and mutualist species interactions, abiotic conditions, human pressure, and numerous other factors. A combination of experiments, large-scale comparative studies, and mechanistic models could help disentangle the effects of defaunation from other anthropogenic forces in the face of the incredible complexity of tropical forest systems. Overall, our synthesis emphasizes the importance of-and inconsistent results when-integrating animal dynamics into carbon cycle models, which is crucial for developing climate change mitigation strategies and effective policies.
    MeSH terms: Animals; Carbon/metabolism; Conservation of Natural Resources*; Trees/physiology; Tropical Climate*; Climate Change*; Carbon Cycle*; Seed Dispersal; Herbivory; Forests; Rainforest*
  17. Midot F, Goh KM, Liew KJ, Lau SYL, Espenberg M, Mander Ü, et al.
    Appl Environ Microbiol, 2025 Jan 31;91(1):e0198624.
    PMID: 39714193 DOI: 10.1128/aem.01986-24
    Tropical peatlands significantly influence local and global carbon and nitrogen cycles, yet they face growing pressure from anthropogenic activities. Land use changes, such as peatland forests conversion to oil palm plantations, affect the soil microbiome and greenhouse gas (GHG) emissions. However, the temporal dynamics of microbial community changes and their role as GHG indicators are not well understood. This study examines the dynamics of peat chemistry, soil microbial communities, and GHG emissions from 2016 to 2020 in a logged-over secondary peat swamp forest in Sarawak, Malaysia, which transitioned to an oil palm plantation. This study focuses on changes in genetic composition governing plant litter degradation, methane (CH4), and nitrous oxide (N2O) fluxes. Soil CO2 emission increased (doubling from approximately 200 mg C m-2 h-1), while CH4 emissions decreased (from 200 µg C m-2 h-1 to slightly negative) following land use changes. The N2O emissions in the oil palm plantation reached approximately 1,510 µg N m-2 h-1, significantly higher than previous land uses. The CH4 fluxes were driven by groundwater table, humification levels, and C:N ratio, with Methanomicrobia populations dominating methanogenesis and Methylocystis as the main CH4 oxidizer. The N2O fluxes correlated with groundwater table, total nitrogen, and C:N ratio with dominant nirK-type denitrifiers (13-fold nir to nosZ) and a minor role by nitrification (a threefold increase in amoA) in the plantation. Proteobacteria and Acidobacteria encoding incomplete denitrification genes potentially impact N2O emissions. These findings highlighted complex interactions between microbial communities and environmental factors influencing GHG fluxes in altered tropical peatland ecosystems.IMPORTANCETropical peatlands are carbon-rich environments that release significant amounts of greenhouse gases when drained or disturbed. This study assesses the impact of land use change on a secondary tropical peat swamp forest site converted into an oil palm plantation. The transformation lowered groundwater levels and changed soil properties. Consequently, the oil palm plantation site released higher carbon dioxide and nitrous oxide compared to previous land uses. As microbial communities play crucial roles in carbon and nitrogen cycles, this study identified environmental factors associated with microbial diversity, including genes and specific microbial groups related to nitrous oxide and methane emissions. Understanding the factors driving microbial composition shifts and greenhouse gas emissions in tropical peatlands provides baseline information to potentially mitigate environmental consequences of land use change, leading to a broader impact on climate change mitigation efforts and proper land management practices.
    MeSH terms: Agriculture; Bacteria/classification; Bacteria/genetics; Bacteria/isolation & purification; Bacteria/metabolism; Carbon/analysis; Carbon/metabolism; Malaysia; Methane/analysis; Methane/metabolism; Nitrogen/analysis; Nitrogen/metabolism; Nitrous Oxide/analysis; Nitrous Oxide/metabolism; Soil/chemistry; Soil Microbiology*; Arecaceae; Wetlands; Carbon Cycle; Nitrogen Cycle; Microbiota*; Forests*
External Links