Browse publications by year: 2025

  1. Zafar M, Sakidin H, Hussain A, Ullah F, Sheremet M, Dzulkarnain I, et al.
    Heliyon, 2025 Jan 15;11(1):e41512.
    PMID: 39844996 DOI: 10.1016/j.heliyon.2024.e41512
    Although oil extraction is indispensable for meeting worldwide energy demands and ensuring industrial sustainability, various hazards are observed. Therefore, this study examined the chemical oil recovery-related environmental consequences concerning water, soil, ecosystem, and human health damages. A numerical analysis explored the mathematical model for oil extraction from unconventional sources by utilising 3D porous prism geometries under high-temperature conditions. This unique methodology utilised environmentally friendly TiO2-SiO2 hybrid nanoparticles, which were not previously investigated. The optimal conditions for oil extraction were then determined by simulations performed at 100 °C, 150 °C, and 200 °C for 2 h, 4 h, 8 h, and 12 h. This study also explored the optimisation of recovery rates by analysing several variables using ANSYS Fluent software, such as flow rate, porosity, and volume fraction. Consequently, these green TiO2-SiO2 nanoparticles presented an oil recovery rate that was 28 % and 6 % higher than water-flooding and conventional monofluid injection techniques, respectively. This outcome suggested that these TiO2-SiO2 nanoparticles could enhance efficiency and minimise environmental damage.
  2. Khan MI, Sufian S, Hassan F, Shamsuddin R, Farooq M
    RSC Adv, 2025 Jan 16;15(3):1989-2010.
    PMID: 39845118 DOI: 10.1039/d4ra05782a
    In this study, a binary composite adsorbent based on activated carbon and phosphoric acid geopolymer foam (ACP) was prepared by combining phosphoric acid geopolymer (PAGP) with activated carbon (AC) and applied for the removal of methylene blue (MB). Activated carbon was thoroughly mixed with a mixture of fly ash and metakaolin in varying ratios, followed by phosphoric acid activation and thermal curing. The ACP adsorbent was characterized using scanning electron microscope (SEM), Fourier transform infrared (FTIR) spectrophotometer, X-ray diffractometer (XRD), surface area analyser (SAP), and thermogravimetric analyser (TGA). Batch analysis was performed to examine the effects of various adsorption parameters including pH (2, 4, 6, 7, 8, and 10), adsorbent dosage (0.06-0.2 g), MB concentration (50-250 mg L-1), contact duration (up to 240 minutes), and temperature (25-55 °C). The ACP with 70% PAGP and 30% AC was found to be the most suitable adsorbent as it maintained its structure and exhibited better MB adsorption. The ACP had a surface area of 47.36 m2 g-1 and a pore size of 5.6 nm and was found to be amorphous in nature. The adsorption equilibrium reached in 240 minutes at pH 7, indicating an efficient adsorption process. The adsorption increased with the initial dye concentration and decreased with the increase in temperature. The ideal parameters for adsorption of MB using ACP include 0.2 g of adsorbent, 25 °C, pH 10, and 240 minutes. The adsorption data fitted well with the Langmuir isotherm, pseudo-second-order (PSO) kinetics model, and three-step intraparticle diffusion (IPD) model. The adsorption capacity calculated using the Langmuir isotherm was 204.8 mg g-1 with an R 2 = 0.989. Thermodynamics parameters showed that the adsorption process was exothermic, energetically favourable, and associated with a decrease in entropy. According to the FTIR findings, pH effect, Langmuir isotherm, PSO kinetics, IPD model, and thermodynamics factors, chemisorption is identified as the predominant process. Different machine learning models, i.e., gaussian process regression (GPR), support vector regression (SVR and SVR-rbf), random forest regression (RFR), decision tree regression (DTR) and artificial neural network (ANN), were trained and tested using adsorption capacity and % removal data. The ANN model (random search) demonstrated better performance compared to other models, achieving an R 2 value of 0.873 for adsorption capacity and 0.799 for % removal on test data.
  3. Ing SK, Lee YH, Ngu NH, Wong KKY, Ismail AMB, Chai CS, et al.
    Breathe (Sheff), 2025 Jan;21(1):240125.
    PMID: 39845436 DOI: 10.1183/20734735.0125-2024
    Patients with multisystemic presentation including respiratory symptoms and generalised lymphadenopathy should alert the clinician to this potential diagnosis https://bit.ly/4eJ0PHT.
  4. Rosmawati R, Sari SF, Asnani A, Embe W, Asjun A, Wibowo D, et al.
    Int J Food Sci, 2025;2025:7568352.
    PMID: 39845694 DOI: 10.1155/ijfo/7568352
    Two plasticizers with distinct properties are carefully studied in this research for their suitability in creating biocomposite edible film products. The study uncovers films' physical, tensile, and biodegradability attributes, using snakehead gelatin and ĸ-carrageenan in different concentrations, with sorbitol or glycerol as plasticizers. The biomaterials of the edible film consist of snakehead gelatin (Channa striata) 2% (w/v); ĸ-carrageenan at concentrations of 1%, 1.5%, and 2% (w/v); and sorbitol/glycerol 15% (v/v). The addition of ĸ-carrageenan up to 2% in the formulation increased the film thickness to 0.046 ± 0.005 mm, tensile strength to 2.05 ± 0.56 MPa, and elongation at break to 35.00% ± 2.92% while decreasing the water vapor transmission rate (WVTR) to 0.17 ± 0.00 g/mm2/h (p < 0.05). The effect of glycerol in the composite did not affect thickness and luminosity (L ∗) (p > 0.05), but the tensile strength increased from 0.18 ± 0.17 to 1.03 ± 0.40 MPa (p > 0.05). Sorbitol increased the value of color difference, elongation at break, and WVTR, namely, 19.77 ± 1.02, 25.20% ± 1.79%, and 0.28 ± 0.02 g/mm2/h, respectively (p < 0.05). The swelling index of the films increased with ĸ-carrageenan concentration, whereas the water content decreased (p < 0.05). The addition of sorbitol reduced the solubility of the film from 71.43% ± 12.39% to 42.67% ± 15.44% (p < 0.05), while glycerol did not affect changes in film solubility (p > 0.05). The presence of sorbitol had no significant effect on the contact angle (p > 0.05) and was more affected by the addition of glycerol at higher ĸ-carrageenan concentrations (p < 0.05). The ability to decompose after 28 days was more remarkable for films containing glycerol than sorbitol. Fourier transform infrared analysis revealed the functional group structures of all samples, indicating that no new compounds were formed in them. The surface structure of the sorbitol-plasticized film was predicted to be rougher and easily cracked, but more compact and dense, while the glycerol-plasticized film tended to be smoother with fainter cracks due to its hygroscopic properties.
  5. Suleiman AK, Ming LC
    J Pharm Policy Pract, 2025;18(1):2449051.
    PMID: 39845746 DOI: 10.1080/20523211.2024.2449051
    Vision 2030 represents Saudi Arabia's strategic socio-economic plan aimed at promoting economic diversification and enhancing living standards. Launched in April 2016, this vision describes an innovative healthcare plan to improve services and outcomes. The merits and drawbacks of public hospitals' 'Model of Care' are examined in this study. This assessment summarises Vision 2030s healthcare revolution. This study explores the innovative 'Model of Care' and its potential to change Saudi healthcare. Prior to Vision 2030, Saudi Arabia's healthcare system grappled with escalating costs and a heavy reliance on foreign labour, signalling a need for transformative change. Vision 2030s patient-centric framework emphasises individualised treatment, and the National Guidelines Center's role in standardising healthcare processes is examined in this review. In addition, we also explored the new initiative of Electronic Health Records and telemedicine services. Additionally, we addressed the challenges associated with it, such as the imperative to address the digital divide and ensure data security. Vision 2030 in Saudi Arabia brought about a significant transformation in the provision of healthcare. Despite the presence of challenges, such as inadequate infrastructure, insufficient personnel, and resistance to change, this vision offers a robust foundation for effectively tackling these concerns. The implementation of these improvements is expected to enhance the calibre and accessibility of healthcare services. This transitional change ensures healthcare is both innovative and inclusive, particularly in socioeconomically disadvantaged regions. This measure is expected to enhance the general welfare and economic success of the population in Saudi Arabia. The future of healthcare in the Kingdom hinges on the effective implementation of evidence-based therapy, collaborative cooperation, and equitable practices. The healthcare sector possesses significant potential for substantial positive transformation, notwithstanding its continuous nature.
  6. Prasetya AIP, Ammarullah MI, Winarni TI, Pramono A, Jamari J, Kamarul T, et al.
    Health Sci Rep, 2025 Jan;8(1):e70305.
    PMID: 39846047 DOI: 10.1002/hsr2.70305
    BACKGROUND AND AIMS: High contact stresses involving the hip have been shown to increase the risk of developing hip osteoarthritis (OA). Although several risk factors have been identified for OA, a holistic approach to predicting contributed factors toward increased hip contact stresses have not been explored. This study was conducted to comprehensively understand the effects of physical activity on high hip contact stress as predisposing factors of OA.

    METHODS: The protocol of this systematic review was registered in PROSPERO with registration number CRD42022296638 and conducted based on PRISMA guidelines. Full articles that matched our inclusion criteria were selected using PubMed, Web of Science, and Scopus search engines and keywords such as "hip contact stress," "hip contact force," and/or "hip contact pressure." Category of factors, experimental design, results of the study, and evidence from each article were analyzed.

    RESULTS: In total 7972 papers were screened, identified, and reviewed. Two independent authors read the collected fulltext of eligible articles resulting in 21 papers that fulfilled the inclusion criteria of this systematic review.

    CONCLUSION: Types of physical activity (n = 21) have correlation with high hip joint contact stress in various manner. Based on the research findings obtained from various inclusion papers, it can be broadly concluded that the more intense the physical activity, such as running and stair climbing, the greater the impact on the increase in hip contact stress values. However, the reviewed studies vary in their methods. This finding suggested that this area is not well investigated and warrants future research.

  7. Ramamoorthi L, Jeyabalan S, Sankar S, Begum MY, Duraipandian C, Sekar M, et al.
    Biomol Concepts, 2025 Jan 01;16(1).
    PMID: 39846213 DOI: 10.1515/bmc-2022-0050
    Seaweeds have been utilized as food, fodder, fertilizer, and medicine since ancient times; nevertheless, they have received only a little attention. In the current work, we extracted the sulfated polysaccharide from a marine source and investigated its anti-arthritic potential in vivo. The isolated and freeze-dried polysaccharide was tested for acute oral toxicity based on OECD 423. This step was followed by investigations on clinical signs and gross pathological alterations seen. A complete Freund's adjuvant-induced arthritis was used to test the in vivo activity in female Sprague-Dawley rats, which were divided into five groups: (1) normal control, (2) arthritic control, (3) methotrexate treatment (0.1 mg/kg), (4) crude sulfated polysaccharide (CSP) (5 mg/kg), and (5) CSP (10 mg/kg). CSP was from the marine brown algae Sargassum ilicifolium from the Gulf of Mannar. The body weight, paw volume, and biochemical markers (alanine aminotransferase, aspartate aminotransferase, creatinine, urea, and C-reactive protein levels) were also measured for each group coupled with histopathological and immunohistochemistry studies. The acute toxicity investigation indicated that the lethal dose of 50% (LD50) of the polysaccharide was more than 2,000 mg/kg. In addition, animals from the methotrexate and CSP (5 mg/kg, p.o.) groups had a substantial reduction in paw volume compared to other treatment groups. Methotrexate and CSP treatment dramatically decreased the levels of the investigated marker enzymes. Histopathology revealed that low-dose CSP (5 mg/kg, p.o.) significantly reduced the severity of synovitis, panniculitis, liver necrosis, inflammatory cell infiltration, and cortical and paracortical necrotic foci in node, compared to the high dose (10 mg/kg, p.o.). Immunohistochemical studies revealed that CSP (5 mg/kg) significantly inhibited pro-inflammatory cytokines such as tumor necrosis factor-alpha, interleukin-2, and CD4 cells. Overall, it can be concluded that a low-dose CSP (5 mg/kg) is an efficient anti-arthritic agent that confers its effects via the cytokine pathway.
    MeSH terms: Animals; Arthritis, Experimental/drug therapy; Arthritis, Experimental/metabolism; Arthritis, Experimental/pathology; Female; Methotrexate/pharmacology; Sulfates/chemistry; Rats, Sprague-Dawley*; Rats
  8. Sreeramareddy CT, Shu Syuen MO, Hon MI, Daher AM
    Nicotine Tob Res, 2025 Jan 22;27(2):254-261.
    PMID: 39110887 DOI: 10.1093/ntr/ntae187
    INTRODUCTION: Data on e-cigarette (EC) and heated tobacco product (HTP) use and the reasons for their use are useful for policy making. We report comparable nationally representative estimates of EC and HTP use and their associated factors.

    AIMS AND METHODS: Global Adult Tobacco Survey data from Indonesia (2021), Kazakhstan (2019), and the Philippines (2021) were analyzed. The weighted prevalence rates and 95% CI of EC and HTP awareness, current use, and ever use, and their distribution by cigarette smoking status were calculated. Binary logistic regression analyses assessed socioeconomic, and tobacco control factors associated with EC and HTP use.

    RESULTS: The prevalence (%) of EC awareness, ever use and current use were 48.7-69.4, 3.6-8.8, and 1.9-3.0, respectively. The prevalence(%) of HTP awareness, ever use and current use were 2.7-21.7, 0.2-2.1, and 0.1-1.2, respectively. The main reasons for EC and HTP use were attractive flavors(45.8%-73.9%), less harmful than smoking(23.0%-70.1%), and enjoyment(40.8%-76.7%). Avoiding going back to smoking(0.9%-54.4%) and quitting smoking(19.4%-49.8%) were less frequently cited reasons (except in the Philippines). EC/HTP use was associated with younger age, higher education and wealth, current/past smoking, exposure to information about the dangers of tobacco use, and advertisements about tobacco products and smoke-free rules at home.

    CONCLUSIONS: The prevalence of EC and HTP use was higher among younger men with higher education and wealth, and current/past smoking. EC and HTP use should be closely monitored. Regulations to restrict the widespread marketing and sales of EC and HTP are needed to prevent the escalation of their use.

    IMPLICATIONS: The population-level data provide the benchmark for future monitoring use of e-cigarettes and HTPs and identify population subgroups for future surveillance in low- and middle-income countries. The association of EC/HTP use with tobacco control-related factors provides leads for policies that should be formulated and implemented to regulate the product contents, marketing, and sales of EC and HTP.

    MeSH terms: Adolescent; Adult; Female; Hot Temperature; Humans; Indonesia/epidemiology; Kazakhstan/epidemiology; Male; Middle Aged; Philippines/epidemiology; Surveys and Questionnaires; Prevalence; Young Adult; Tobacco Use/epidemiology; Electronic Cigarettes/statistics & numerical data; Vaping/epidemiology
  9. Ab Rahman N, Chellapan K, Ong PY, Adnan A, Md Din N
    Retina, 2025 Feb 01;45(2):310-317.
    PMID: 39442016 DOI: 10.1097/IAE.0000000000004297
    PURPOSE: To evaluate systemic vascular fitness measured by finger photoplethysmography in diabetic retinopathy (DR).

    METHODS: This was a cross-sectional observational study on patients with Type II diabetes mellitus from October 2020 to May 2021. Data collected include systolic/diastolic blood pressure, visual acuity, glycated hemoglobin, and central macular thickness. Diabetic retinopathy severity was categorized using the Early Treatment Diabetic Retinopathy Study classification. Photoplethysmography signals were acquired using pulse-oximeter modules (OEM-60; Dolphin Medical, Inc) measured for 90 seconds at 275 Hz sampling rate and 16-bit resolution, which records photoplethysmography fitness index, vascular risk prediction index, and vascular age.

    RESULTS: One hundred and forty-one patients were equally distributed into six DR categories. Mean age was 58.8 ± 9.9 years, with female-to-male ratio of 1.27. There were significant differences in mean systolic (125.5 ± 10.0 mmHg, P = 0.007) and diastolic blood pressure (80.0 ± 8.8 mmHg), mean glycated hemoglobin (7.6 ± 1.9%, P = 0.005), median log unit of minimal angle of resolution (0.3, interquartile range: 0.2-0.5, P < 0.001), and central macular thickness ( P = 0.003) across DR severity. Significant differences were also seen in photoplethysmography fitness index ( P = 0.001), vascular risk prediction index ( P < 0.001), and vascular age ( P = 0.001), with poorer values in severe compared with mild/moderate DR. After adjusting for age, blood pressure, and glycated hemoglobin, photoplethysmography fitness reduces by 3.3% (regression coefficient, b = -3.27, P < 0.001), vascular age increases by 2.5 years ( b = 2.54, P = 0.002), and vascular risk prediction index increases by 3.1 ( b = 3.08, P < 0.001) with every DR worsening.

    CONCLUSION: More severe DR stages were associated with poorer photoplethysmography vascular markers.

    MeSH terms: Aged; Blood Pressure/physiology; Cross-Sectional Studies; Diabetes Mellitus, Type 2/complications; Diabetes Mellitus, Type 2/physiopathology; Female; Hemoglobin A, Glycosylated/metabolism; Humans; Male; Middle Aged; Visual Acuity/physiology
  10. Loganath K, Craig NJ, Everett RJ, Bing R, Tsampasian V, Molek P, et al.
    JAMA, 2025 Jan 21;333(3):213-221.
    PMID: 39466640 DOI: 10.1001/jama.2024.22730
    IMPORTANCE: Development of myocardial fibrosis in patients with aortic stenosis precedes left ventricular decompensation and is associated with an adverse long-term prognosis.

    OBJECTIVE: To investigate whether early valve intervention reduced the incidence of all-cause death or unplanned aortic stenosis-related hospitalization in asymptomatic patients with severe aortic stenosis and myocardial fibrosis.

    DESIGN, SETTING, AND PARTICIPANTS: This prospective, randomized, open-label, masked end point trial was conducted between August 2017 and October 2022 at 24 cardiac centers across the UK and Australia. Asymptomatic patients with severe aortic stenosis and myocardial fibrosis were included. The final date of follow-up was July 26, 2024.

    INTERVENTION: Early valve intervention with transcatheter or surgical aortic valve replacement or guideline-directed conservative management.

    MAIN OUTCOMES AND MEASURES: The primary outcome was a composite of all-cause death or unplanned aortic stenosis-related hospitalization in a time-to-first-event intention-to-treat analysis. There were 9 secondary outcomes, including the components of the primary outcome and symptom status at 12 months.

    RESULTS: The trial enrolled 224 eligible patients (mean [SD] age, 73 [9] years; 63 women [28%]; mean [SD] aortic valve peak velocity of 4.3 [0.5] m/s) of the originally planned sample size of 356 patients. The primary end point occurred in 20 of 113 patients (18%) in the early intervention group and 25 of 111 patients (23%) in the guideline-directed conservative management group (hazard ratio, 0.79 [95% CI, 0.44-1.43]; P = .44; between-group difference, -4.82% [95% CI, -15.31% to 5.66%]). Of 9 prespecified secondary end points, 7 showed no significant difference. All-cause death occurred in 16 of 113 patients (14%) in the early intervention group and 14 of 111 (13%) in the guideline-directed group (hazard ratio, 1.22 [95% CI, 0.59-2.51]) and unplanned aortic stenosis hospitalization occurred in 7 of 113 patients (6%) and 19 of 111 patients (17%), respectively (hazard ratio, 0.37 [95% CI, 0.16-0.88]). Early intervention was associated with a lower 12-month rate of New York Heart Association class II-IV symptoms than guideline-directed conservative management (21 [19.7%] vs 39 [37.9%]; odds ratio, 0.37 [95% CI, 0.20-0.70]).

    CONCLUSIONS AND RELEVANCE: In asymptomatic patients with severe aortic stenosis and myocardial fibrosis, early aortic valve intervention had no demonstrable effect on all-cause death or unplanned aortic stenosis-related hospitalization. The trial had a wide 95% CI around the primary end point, with further research needed to confirm these findings.

    TRIAL REGISTRATION: ClinicalTrials.gov Identifier: NCT03094143.

    MeSH terms: Aged; Aged, 80 and over; Aortic Valve/pathology; Aortic Valve/surgery; Aortic Valve Stenosis*; Cause of Death; Female; Fibrosis*; Hospitalization*; Humans; Male; Myocardium/pathology; Prospective Studies; Heart Valve Prosthesis Implantation; Intention to Treat Analysis; Asymptomatic Diseases; Early Medical Intervention; Transcatheter Aortic Valve Replacement; Conservative Treatment
  11. Quan G, Xiao H, Chen Y
    Sci Rep, 2025 Jan 17;15(1):2259.
    PMID: 39833365 DOI: 10.1038/s41598-025-86432-x
    This study explores the impact of state and trait anxiety on perceived stress in athletes in high-risk sports, focusing on the mediating role of cognitive reappraisal and the moderating effect of sports psychological skills. Data were collected from athletes in sports like Boxing, Sanda, Mixed Martial Arts, Freestyle Skiing, and Ski Jumping. The analysis, conducted using the PROCESS macro revealed that both state and trait anxiety significantly predict perceived stress (β = 0.331, p 
    MeSH terms: Adaptation, Psychological*; Adolescent; Adult; Athletic Injuries/psychology; Cognition/physiology; Female; Humans; Male; Sports/psychology; Young Adult
  12. Kuo YY, Chiu SQ, Chang WP, Chen CY, Chen CH, Liaw CK, et al.
    J Orthop Surg Res, 2025 Jan 20;20(1):73.
    PMID: 39833829 DOI: 10.1186/s13018-024-05392-6
    BACKGROUND: Full-thickness cartilage defects have a significant impact on the function of joints in young adults, and the treatment of cartilage defects has been a challenge, as cartilage tissue is an avascular tissue. This study aimed to compare the clinical and radiological outcomes of Biphasic Cartilage Repair Implant (BiCRI) and microfracture treatments for knee cartilage defects.

    METHODS: This randomized controlled clinical trial enrolled patients with symptomatic knee chondral lesions smaller than 3 cm2. They were randomized to either the BiCRI (n = 11) or microfracture (n = 10) treatment groups. BiCRI or microfracture surgical procedures were performed on the patients, who were subsequently followed for a period of five years. Primary outcome measures included the International Knee Documentation Committee (IKDC) score, Knee Injury and Osteoarthritis Outcome Score (KOOS), Visual Analog Scale (VAS) score, Magnetic Resonance Imaging (MRI) measured cartilage thickness, and the Magnetic Resonance Observation of Cartilage Repair Tissue (MOCART) score.

    RESULTS: 21 patients were enrolled, who were subsequently followed for a period of five years. Both BiCRI and microfracture treatments demonstrated significant improvements in IKDC, KOOS and VAS scores, with no significant differences between the two. MRI analysis indicated a significant increase in minimum cartilage thickness with BiCRI treatment (median of difference: 1 mm, P = 0.026)), in contrast to the nonsignificant change in the microfracture group (median of difference: 1 mm, P = 0.102). The MOCART scores revealed a significant increase percentage of isointense signal intensity identical to the adjacent articular cartilage (P = 0.03) in the BiCRI group from the 2-year to the 5-year mark, while the scores remained stable in the microfracture group. Moreover, the BiCRI technique displayed superior performance in graft infill at 5 years (P = 0.008), border integration at 5 years (P = 0.04), surface contour at 2 years (P = 0.04) compared to microfracture.

    CONCLUSIONS: Both BiCRI and microfracture treatments showed significant effectiveness in improving clinical outcomes in patients with small symptomatic articular cartilage defects of the knee, with the BiCRI group demonstrating a superior radiological outcome than microfracture, over a five-year period. However, the sample size of our study is relatively small to reach a definite conclusion, and further studies with larger sample size and longer follow up are recommended. Trial registration The trial was registered on ClinicalTrials.gov under the identifier NCT01477008.

    MeSH terms: Adolescent; Adult; Female; Follow-Up Studies; Humans; Knee Injuries/surgery; Knee Joint/surgery; Magnetic Resonance Imaging; Male; Prospective Studies; Time Factors; Treatment Outcome; Young Adult
  13. Ding YC, Tang GW, Zhao HY, Liu JM, Fan TH, Peng YC, et al.
    ACS Appl Mater Interfaces, 2025 Jan 29;17(4):6857-6866.
    PMID: 39834073 DOI: 10.1021/acsami.4c22615
    Radiative cooling, a passive cooling technology, functions by reflecting the majority of solar radiation (within the solar spectrum of 0.3-2.5 μm) and emitting thermal radiation (within the atmospheric windows of 8-13 μm and 16-20 μm). Predominantly, synthetic polymers are effectively utilized for radiative cooling while posing potential environmental hazards due to their complex components, toxicity, or nonbiodegradation. Bacterial cellulose, a natural and renewable biopolymer, stands out due to its environmentally friendly, scalability, high purity, and significant infrared emissivity. In this work, we developed a bacterial cellulose-based composite film (BCF) with a cross-linked network structure by a facile agitation spraying method to achieve enhanced and sustainable radiative cooling performance. The BCF exhibited superior optical properties and environmental tolerance, with a notable infrared emissivity of 94.6%. As a result, the thermal emitter demonstrates a substantial subambient cooling capacity (11:00 to 13:00, maximum drop of 7.15 °C, average drop of 4.85 °C; 22:00 to 2:00, maximum drop of 2.7 °C, average drop of 2.32 °C). Additionally, the BCF maintained stable emissivity after 240 h of continuous UV irradiation. Furthermore, BCF can effectively preserve the freshness of fruits under intense solar irradiation. Hence, BCF with high radiative cooling performance presents a broad application prospect in building energy conservation, solar cells efficiency enhancement, and food transportation packaging.
  14. Cai Z, Yuan X, Zhong G, Zhang T, He J, Dang Y, et al.
    J Agric Food Chem, 2025 Jan 29;73(4):2623-2633.
    PMID: 39834201 DOI: 10.1021/acs.jafc.4c08913
    Lipoteichoic acid synthase (LtaS) is crucial for the biosynthesis of lipoteichoic acid (LTA) in lactic acid bacteria (LAB), where LTA plays a key role in bacterial adhesion, immune modulation, and maintaining cell integrity. This study explores the regulation of LtaS activity in Lactiplantibacillus plantarum, examining the effects of factors such as temperature, pH, and metal ions on enzyme activity. Molecular docking was used to identify critical amino acids at the enzyme's active site, and site-directed mutagenesis confirmed the role of five key residues (Glu-259, Thr-303, Asn-353, Arg-360, and His-420) in LtaS activity. Among them, Thr-303 plays a pivotal role, followed by Glu-259 and His-420. Conservation analysis revealed that these active-site residues are highly conserved across LAB species. These findings provide valuable insights into the functional properties of LtaS, offering potential for enhancing the efficacy of LAB-based probiotics and improving their therapeutic benefits in health applications.
    MeSH terms: Amino Acid Sequence; Amino Acids/metabolism; Amino Acids/chemistry; Lipopolysaccharides*; Mutagenesis, Site-Directed; Catalytic Domain; Lactobacillus plantarum/enzymology; Lactobacillus plantarum/genetics; Lactobacillus plantarum/metabolism; Lactobacillus plantarum/chemistry; Molecular Docking Simulation*
  15. Tagiling N, Lee YY, Mohd Rohani MF, Udin MY, Abdul Aziz A, Muhamad SN, et al.
    PMID: 39835631 DOI: 10.1111/nmo.15006
    BACKGROUND: Gastric accommodation (GA) testing is gaining clinical recognition as novel and minimally invasive modalities emerge. We investigated the feasibility of hybrid nuclear imaging volumetry (SPECT/CT) and combined high-resolution manometry-nutrient drink test (HRM-NDT) to assess GA.

    METHODS: In this non-randomized pilot study, [99mTc]NaTcO4 gastric SPECT/CT (250 mL protocol) and proximal gastric HRM-NDT (~60 mL/min protocol) were performed separately within 30 days using Ensure Gold test meal (1.05 kcal/mL; Abbott). GA parameters were measured, and their preliminary associations were examined using Spearman's ρ and Hoeffding's D correlation tests. Data were presented as median ± normalized median absolute deviation.

    KEY RESULTS: Twenty healthy, asymptomatic individuals (11 females; 23.5 ± 2.2 years, 23.7 ± 2.2 kg/m2) completed both procedures without serious adverse events and interrupted sessions. The accommodation volume and postprandial-to-fasting volume ratio from SPECT/CT were 325.8 ± 28.5 mL and 5.31 ± 1.28, respectively. During HRM-NDT, the nadir-intragastric pressure (IGP) was -6.6 ± 3.6 mmHg at an ingested volume of 360.0 ± 177.9 mL, and the area-under-curve of IGP was -1566.0 ± 1596.8 mmHg·mL. The maximum tolerated volume for reaching satiety/maximum discomfort was 450.0 ± 177.9 mL, and the area-under-curve of satiation score was 900.0 ± 266.9 satiation-unit·mL. The area-under-curve of IGP showed significant associations with maximum tolerated volume (ρ: -0.702; D: 0.234) and the area-under-curve of satiation score (D: 0.119): all p 

  16. Marhaendra LIA, Rosandi Y, Gazzali AM, Novitasari D, Muchtaridi M
    Drug Dev Ind Pharm, 2025 Mar;51(3):193-208.
    PMID: 39835740 DOI: 10.1080/03639045.2025.2457387
    OBJECTIVE: This article provides a substantial review of recent research and comparison on molecular dynamics potentials to determine which are most suitable for simulating the phenomena in graphene-based nanomaterials (GBNs).

    SIGNIFICANCE: GBNs gain significant attention due to their remarkable properties and potential applications, notably in nanomedicine. However, the physical and chemical characteristics toward macromolecules that justify their nanomedical applications are not yet fully understood. The molecular interaction through molecular dynamic simulation offers the benefits for simulating inorganic molecules like GBNs, with necessary adjustments to account for physical and chemical interactions, or thermodynamic conditions.

    METHOD: In this review, we explore various molecular dynamics potentials (force fields) used to simulate interactions and phenomena in graphene-based nanomaterials. Additionally, we offer a brief overview of the benefits and drawbacks of each force fields that available for analysis to assess which one is suitable to study the molecular interaction of graphene-based nanomaterials.

    RESULT: We identify and compare various molecular dynamics potentials that available for analyzing GBNs, providing insights into their suitability for simulating specific phenomena in graphene-based nanomaterials. The specification of each force fields and its purpose can be used for further application of molecular dynamics simulation on GBNs.

    CONCLUSION: GBNs hold significant promise for applications like nanomedicine, but their physical and chemical properties must be thoroughly studied for safe clinical use. Molecular dynamics simulations, using either reactive or non-reactive MD potentials depending on the expected chemical changes, are essential for accurately modeling these properties, requiring careful selection based on the specific application.

    MeSH terms: Thermodynamics; Molecular Dynamics Simulation*
  17. Malvi A, Shabil M, Khatib MN, R R, Kaur M, Srivastava M, et al.
    J Clin Hypertens (Greenwich), 2025 Jan;27(1):e14977.
    PMID: 39835844 DOI: 10.1111/jch.14977
    Sex hormone-binding globulin (SHBG) regulates sex hormone availability and is influenced by metabolic factors. Variations in SHBG levels during pregnancy may affect the development of hypertensive disorders such as gestational hypertension (GH) and preeclampsia (PE). This systematic review and meta-analysis explores the potential of SHBG as a biomarker for predicting GH and PE. A search of PubMed, Embase, and Web of Science was conducted to identify studies evaluating the association between SHBG levels and the risk of HDP, including GH and PE. Inclusion criteria encompassed observational studies reporting quantitative risk estimates (risk ratios, odds ratios, or hazard ratios) for SHBG levels. Results were pooled using a random-effects meta-analysis in R software (V 4.4), with the I2 statistic assessing heterogeneity. Eight studies were included in the systematic review from a total of 592 screened records. The association between SHBG levels and the risk of any HDP showed a pooled OR of 0.875 (95% CI: 0.772-0.993), for PE 0.890 (95% CI: 0.767-1.032), and for GH 0.729 (95% CI: 0.442-1.205), suggesting significant association between SHBG and HDP. Sensitivity analysis validated the robustness of the findings. This meta-analysis found potential significant association between higher SHBG levels and risk of HDP. Further high-quality research is required to better understand the role of SHBG in pregnancy-related hypertensive disorders. Future studies should consider larger sample sizes, more precise measurement techniques, and explore potential confounding factors to clarify the potential utility of SHBG as a biomarker for predicting GH and PE.
    MeSH terms: Adult; Female; Humans; Pregnancy; Risk Factors
  18. Mahmod AI, Govindaraju K, Lokanathan Y, Said NABM, Ibrahim B
    Stem Cells Dev, 2025 Mar;34(5-6):99-116.
    PMID: 39836384 DOI: 10.1089/scd.2024.0195
    Hypertension, commonly known as high blood pressure, is a significant health issue that increases the risk of cardiovascular diseases, stroke, and renal failure. This condition broadly encompasses both primary and secondary forms. Despite extensive research, the underlying mechanisms of systemic arterial hypertension-particularly primary hypertension, which has no identifiable cause and is affected by genetic and lifestyle agents-remain complex and not fully understood. Recent studies indicate that an imbalance in gut microbiota, referred to as dysbiosis, may promote hypertension, affecting blood pressure regulation through metabolites such as short-chain fatty acids and trimethylamine N-oxide. Current antihypertensive medications face limitations, including resistance and adherence issues, highlighting the need for novel therapeutic approaches. Stem cell therapy, an emerging field in regenerative medicine, shows promise in addressing these challenges. Stem cells, with mesenchymal stem cells being a prime example, have regenerative, anti-inflammatory, and immunomodulatory properties. Emerging research indicates that stem cells can modulate gut microbiota, reduce inflammation, and improve vascular health, potentially aiding in blood pressure management. Research has shown the positive impact of stem cells on gut microbiota in various disorders, suggesting their potential therapeutic role in treating hypertension. This review synthesizes the recent studies on the complex interactions between gut microbiota, stem cells, and systemic arterial hypertension. By offering a thorough analysis of the current literature, it highlights key insights, uncovers critical gaps, and identifies emerging trends that will inform and guide future investigations in this rapidly advancing field.
    MeSH terms: Gastrointestinal Microbiome*; Animals; Humans; Stem Cells/metabolism; Stem Cell Transplantation/methods; Dysbiosis/microbiology; Dysbiosis/therapy
  19. Harding KG, Blow M, Ashton F, Bosanquet DC
    Adv Skin Wound Care, 2025 01 20;38(1):25-30.
    PMID: 39836553 DOI: 10.1097/ASW.0000000000000261
    OBJECTIVE: To determine if intermittent neuromuscular electrostimulation (NMES) of the common peroneal nerve increases microvascular flow and pulsatility in and around the wound bed of patients with combined venous and arterial etiology.

    METHODS: Seven consenting participants presenting with mixed etiology leg ulcers participated in this study. Microvascular flow and pulsatility was measured in the wound bed and in the skin surrounding the wound using laser speckle contrast imaging. Measurements were made at baseline and when the venous pumps of the leg were activated by 1 Hz intermittent neuromuscular stimulation of the common peroneal nerve. The nerve was stimulated transdermally at the head of the fibula.

    RESULTS: When activated by NMES, wound bed flux increased by 38% (95% CI, 11%-73%; P = .023), and periwound flux increased by 19% (95% CI, 9%-32%; P = .009). Pulsatility increased in the wound bed by 214% (95% CI, 51%-985%; P = .017) and in the periwound by 122% (95% CI, 38%-299%; P = .014).

    CONCLUSIONS: The results indicate that NMES is effective in augmenting microvascular flow in leg ulcers with combined venous and arterial etiology.

    MeSH terms: Aged; Aged, 80 and over; Female; Humans; Male; Middle Aged; Peroneal Nerve/physiopathology; Regional Blood Flow/physiology; Wound Healing/physiology; Treatment Outcome
  20. Tay YL, Ahmad MA, Mohamad Yahaya NH, Ajit Singh DK
    PLoS One, 2025;20(1):e0314869.
    PMID: 39836628 DOI: 10.1371/journal.pone.0314869
    BACKGROUND: Photobiomodulation, specifically high-energy photobiomodulation therapy (H-PBMT), is gaining recognition as a promising non-invasive intervention for managing knee osteoarthritis (KOA). While H-PBMT has demonstrated effectiveness in reducing pain and improving physical function, most evidence to date focuses on short-term symptomatic relief. The potential for H-PBMT to offer sustained benefits and modify the underlying progression of KOA remains insufficiently explored, warranting further investigation.

    OBJECTIVE: This study aims to assess the short-term and sustained effects of H-PBMT combined with rehabilitation exercises in patients with mild to moderate KOA, focusing on knee radiographic morphological changes over a 3-month follow-up period.

    METHODS: This protocol outlines a parallel-group, randomized, double-blind, placebo-controlled trial. Fifty participants with mild to moderate KOA (based on the Kellgren-Lawrence classification) will be randomly assigned to either the active H-PBMT plus exercise group (H-PBMT+E, n = 25) or the placebo photobiomodulation plus exercise group (PL+E, n = 25). Both groups will undergo an 8-week intervention, consisting of conventional rehabilitation exercises paired with either active or placebo photobiomodulation. H-PBMT will be delivered using the BTL-6000 HIL device with a 1064 nm wavelength, providing a total energy dose of 3190 J per 15-minute session. The treatment protocol includes both pulse mode (25 Hz, 5 W, 190 J) for analgesia and continuous mode (5 W, 3000 J) for biostimulation. Participants will be blinded to their group allocation through the use of a placebo device that mimics the active treatment without emitting therapeutic energy. Additionally, the outcome assessors will be blinded to the group allocations to ensure unbiased evaluation of the trial outcomes. The primary outcome is the Knee Injury and Osteoarthritis Outcome Score. Secondary outcomes include the Timed Up-and-Go test, Numerical Pain Rating Scale, and knee X-rays. Outcomes will be evaluated at baseline, immediately post-intervention (week 8), and at 3-month follow-up (week 20). Data will be analyzed according to the intention-to-treat principle, with a two-way repeated measures ANOVA used to assess time, group, and interaction effects.

    CONCLUSION: This study is expected to provide valuable insights into the sustained effects and potential disease-modifying properties of combining H-PBMT with rehabilitation exercises in managing KOA. The findings could inform more effective treatment protocols, improving rehabilitation outcomes and patient quality of life.

    TRIAL REGISTRATION: Australian New Zealand Clinical Trials Registry (ACTRN12624000699561p).

    MeSH terms: Aged; Double-Blind Method; Female; Humans; Male; Middle Aged; Pain/rehabilitation; Treatment Outcome
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