Browse publications by year: 2025

  1. Gandhi AK, Chopra S, Rastogi M, Mallick I, Cruz MC, Yasuda K, et al.
    JCO Glob Oncol, 2025 Jan;11:e2400349.
    PMID: 39819125 DOI: 10.1200/GO-24-00349
    PURPOSE: Head and neck cancers (HNCs) are in general treated with conventional fractionation regimen of 1.8-2 Gy per fraction. Altered fractionation (ALFT) strategies such as hypofractionation radiotherapy (HYPO-RT), accelerated fractionation radiotherapy (AFRT), and hyperfractionation radiotherapy (HFRT) have not been practiced uniformly across centers in different parts of the world. Countries in Asia share common cancer demographics, and we designed this survey for Federation of Asian Radiation Oncology (FARO) member countries to understand the usage and challenges in the delivery of ALFT in HNCs.

    MATERIALS AND METHODS: A 21-point electronic survey (Federation of Asian Radiation Oncology Research Network [FERN]-S-005) was designed by the FERN and was circulated through the FARO research secretariat to the FARO council member countries and the responses were collected between August and November 2023.

    RESULTS: Twelve of 14 member countries (85.7%) responded to the survey. Twenty-seven responses were received and 78% of the respondents belonged to government/teaching academic institute. 4/27 (14.8%) reported never using HYPO-RT for any of the clinical subsite of HNCs, while the majority (85.2%) used it for glottic cancers and 22% also used it for postoperative setting. Majority (77.7%) used a fractionation schedule with dose per fraction ranging between 2.2 and 2.5 Gy. 6/27 (22.2%) used AFRT for definitive setting and five of these also used concurrent chemoradiotherapy. 4/27 (14.8%) centers reported using HFRT. The most common reason (62.9%) for the limited usage of AFRT/HFRT was reported to be logistical, such as unavailability of machine slots, patient load, and so on.

    CONCLUSION: The result of the survey suggests that among the ALFT strategies for HNCs, HYPO-RT schedules have common interest and feasibility among the FARO member countries and also highlights the challenges in the delivery of AFRT/HFRT in the Asian region.

    MeSH terms: Dose Hypofractionation; Asia; Cross-Sectional Studies; Humans; Surveys and Questionnaires; Dose Fractionation*
  2. Chandran R, Mohd Tohit ER, Stanslas J, Salim N, Tuan Mahmood TM
    Curr Drug Deliv, 2025 Jan 15.
    PMID: 39819406 DOI: 10.2174/0115672018346286241121052105
    INTRODUCTION: Poly(methyl vinyl ether co-maleic acid) (PMVE/MA) hydrogel microneedles (HMN) are investigated for transdermal delivery of macromolecular drugs owing to their biocompatibility and super-swelling properties. However, the drug delivery efficacy reduces with increasing molecular weight due to the entrapment within the HMN matrices. Furthermore, integrating external drug reservoirs extends the drug diffusion path and reduces the efficiency of drug permeation.

    METHODS: A direct drug loading approach in the HMN matrix was introduced in this work following a pH modification step. The effect of pH modification on the physicochemical properties of HMN was studied. Then, bovine serum albumin (BSA), a model protein, was loaded into the pH-modified HMN, and the morphological changes in HMN and protein stability were also assessed. Finally, the efficacy of BSA-loaded HMN in the transdermal delivery was evaluated ex vivo.

    RESULTS: A significant increase in swelling was recorded following the pH modification of HMN (p < 0.001). The structure of pH-modified hydrogel was highly porous, and ATR-FTIR spectra indicated a shift in the carboxylic peak. The secondary structure of BSA loaded in the pH-modified HMN was also preserved. The BSA-loaded HMN mediated a sustained ex-vivo drug release with a cumulative release of 64.70% (3.88 mg) in 24 h.

    CONCLUSION: Hence, the model drug-incorporated PMVE/MA HMN system shows potential for sustainable transdermal delivery of proteins.

  3. Noor NS, Hamid SBS
    Curr Mol Med, 2025 Jan 15.
    PMID: 39819413 DOI: 10.2174/0115665240347014241203065055
    BACKGROUND: A biocompatible polymeric nanoparticle, TQ-PLGA-PF68, was developed through the interaction of the phytochemical thymoquinone (TQ) encapsulated in poly(L-lactide-co-glycolide)-b-poly(ethylene glycol) (PLGA-PEG) with Pluronics F68. So far, this combination has not been assessed on breast cancer cells resistant to anti-cancer drugs. Therefore, this study aimed to assess the cell death caused by TQ-PLGA-PF68 nanoparticles, particularly in resistant breast cancer cell lines expressing estrogen receptor (ER) positivity, such as TamR MCF-7.

    METHODS: The antiproliferative activity of TQ-PLGA-PF68 nanoparticles was measured using the MTS assay. The cytotoxic effects were further evaluated through colony formation assay and scratch-wound healing assay. Terminal deoxynucleotidyl transferase dUTP Nick End Labeling (TUNEL) assay was performed to determine the characteristics of the apoptosis as well as cell cycle arrest induced by TQ-PLGA-PF68 nanoparticles. The localization of these nanoparticles in the cells was examined using Transmission Electron Microscopy (TEM).

    RESULTS: With a TQ concentration of 58.5 μM encapsulated within the nanoparticles, cytotoxicity analysis revealed a significant inhibition of cell proliferation (p<0.05). This finding was corroborated by the results of the colony formation assay. Treatment with TQ-PLGA-PF68 nanoparticles significantly decreased the number of surviving TamR MCF-7 cells by 35% (p<0.001) compared to untreated TamR MCF-7 cells. Concurrently, the scratch-wound healing assay indicated a closure rate of 50% versus >80% (p<0.05) in untreated TamR MCF-7 cells at 12 hours post-wounding. The TUNEL assay successfully confirmed the apoptosis characteristics associated with cell cycle arrest. TEM observation confirmed the cellular internalization of these nanoparticles, suggesting the in vitro therapeutic potential of the formulation.

    CONCLUSION: In this study, a significant functional change in TamR MCF-7 cells induced by the TQ nanoparticles was observed. The unique incorporation of TQ into the PLGA-PEG and Pluronics F68 formulation preserved its bioactivity, thereby reducing the migratory and proliferative traits of drug-resistant cells. This discovery may pave the way for exploring the application of biocompatible polymeric TQ nanoparticles as a novel therapeutic approach in future studies pertaining to resistant breast cancer.

  4. Sheferaw WE, Ogunmola GA, Marzo RR, Abebaw S, Belay A, James BC, et al.
    BMC Pediatr, 2025 Jan 16;25(1):35.
    PMID: 39819552 DOI: 10.1186/s12887-025-05400-6
    BACKGROUND: Despite numerous government nutrition-specific and sensitive interventions, undernutrition (e.g., underweight) remains the major public health concern among under-five-year-old children in Ethiopia. Therefore, this study aimed to assess underweight and associated factors among children in Ethiopia using 2016 EDHS data.

    METHOD: The current study used 9,013 children under five years old. An ordinal logistic regression (e.g., proportional odds model) was applied to determine the associated risk factors of being underweight. The current study used SAS software version 9.4 at the 5% significance level.

    RESULTS: The prevalence of underweight was 25.3%. Variables such as children's sex, place of residence, whether the child is twin at birth, breastfeeding status, size of children at birth, childbirth order, employment status of mothers, parents' educational level, children's age groups, the incidence of diarrhea in the past two weeks, and baby fortified food were statistically associated with underweight among under-five age in years.

    CONCLUSIONS: Underweight among under-five children is predicted by place of residence. In addition, there is a regional disparity of underweight among children. Therefore, further effort is needed to improve health education in children's welfare and health facilities to enhance patients' understanding of proper information and feeding.

    MeSH terms: Child, Preschool; Cross-Sectional Studies; Ethiopia/epidemiology; Female; Health Surveys*; Humans; Infant; Male; Risk Factors; Socioeconomic Factors; Child Nutrition Disorders/epidemiology; Prevalence; Logistic Models; Malnutrition/epidemiology
  5. Zhang D, Tang M, Tang N, Earnest BSP, Ali Abdou IEM
    Coron Artery Dis, 2025 Jan 20.
    PMID: 39820077 DOI: 10.1097/MCA.0000000000001498
    BACKGROUND: The Geriatric Nutritional Risk Index (GNRI), derived from serum albumin levels and body weight relative to ideal body weight, is a novel tool for assessing nutritional status. This meta-analysis explored the association between GNRI and the clinical outcomes in patients with acute coronary syndrome (ACS).

    METHODS: We systematically searched PubMed, Embase, and Web of Science for studies evaluating the GNRI in patients with ACS. Inclusion criteria were observational studies reporting all-cause mortality or major adverse cardiovascular events (MACEs) among ACS patients categorized by low versus normal GNRI. Data extraction and quality assessment were independently performed by two authors, utilizing a random-effects model to account for potential heterogeneity.

    RESULTS: Eleven cohort studies, encompassing 18 616 patients with ACS, were included. A low GNRI was associated with significantly increased risks of all-cause mortality (RR, 1.95; 95% CI, 1.63-2.34; P 0.05). Sensitivity analyses conducted by sequentially excluding individual studies confirmed the stability of these results.

    CONCLUSION: A low GNRI at the time of admission is a significant predictor of increased all-cause mortality and MACEs in patients with ACS.

  6. AbuBakar N, Behnke JM, Sahimin N, Kang X, Mohd Shahar SN, Lim YAL, et al.
    PLoS One, 2025;20(1):e0317349.
    PMID: 39820186 DOI: 10.1371/journal.pone.0317349
    Dengue remains the most rapidly advancing vector-borne disease in the world, and while the disease burden is predominantly in low-to-middle-income countries, the association with poverty remains in question. Consequently, a study was undertaken to evaluate the prevalence of anti-dengue antibodies among individuals residing in the People's Housing Program (PPR), a government-sponsored low-cost housing initiative targeting low-income earners. This type of public housing often faces challenges, including substandard housing facilities. Therefore this study took into consideration several social determinants of health, including the economic, environmental, and social conditions that contribute towards dengue transmission. The research was conducted over a period of 18 months across 14 PPRs in Klang Valley, Malaysia. Overall seroprevalence of anti-dengue immunoglobulin G (IgG) was 78.2% (CL: 72.5-83.1) among the 436 residents who participated in the study, while seroprevalence of anti-dengue IgM was 0.9% (CL: 0.2-3.2). Log-linear statistical models with the presence/absence of anti-dengue IgG and individual factors showed significant associations of anti-dengue IgG with age, income, location, and waste bin conditions, but ethnicity was just at the wrong side of the cut-off for significance. However, a multifactorial model, in which all relevant factors were taken into account, showed that location and ethnicity were the key risk factors. For anti-dengue IgM, the only significant association was with the presence of stagnant water bodies around the compounds. Findings from this study highlight an acute need for improvements in the environmental and societal health of those residing in PPRs in locations that are particularly at risk, and continuous community empowerment to ensure that the preventive measures taken to eradicate dengue are locally sustainable.
    MeSH terms: Adolescent; Adult; Aged; Antibodies, Viral/blood; Dengue Virus/immunology; Female; Humans; Immunoglobulin G/blood; Immunoglobulin M/blood; Malaysia/epidemiology; Male; Middle Aged; Poverty; Public Housing*; Seroepidemiologic Studies; Young Adult
  7. Rif'atunnailah MI, Mei-Chan C, Wan Ling L, Tajunisah I, Mohd Iman SS, Thandar Soe Sumaiyah J, et al.
    Health Educ Res, 2025 Feb 19;40(1).
    PMID: 39820426 DOI: 10.1093/her/cyae045
    Diabetic retinopathy (DR) may develop into sight-threatening DR and vision loss if early intervention is not carried out. This study was aimed to assess the effectiveness of DR health education program for patients with type 2 diabetes mellitus (T2DM). The quasi-experimental research design was applied. The intervention group underwent a web-based DR health education program while the control group was followed up the usual way at an ophthalmology clinic for 1 year. Data were analysed using descriptive statistics, repeated measures ANOVA and general linear model to evaluate the mean difference between groups. A total of 180 patients with T2DM were enrolled in the study, with equal number in the control and intervention groups, respectively, with 28% of dropout rate. There was a significant mean difference in knowledge score [F (1178)  = 116.57, P = 0.001], diabetes self-care [F (1178)  = 116.57, P = 0.001] and visual-related quality of life [F (1178)  = 12.70), P = 0.001] between the control and the intervention groups. The intervention group scored the highest in all three categories. Educational interventions positively affected adherence to self-care and visual-related quality of life in type 2 diabetic patients as shown in this study. DRHEP should be considered an added benefit in T2DM management, starting with comprehensive care enrollment.
    MeSH terms: Adult; Aged; Female; Health Education; Humans; Health Knowledge, Attitudes, Practice; Male; Middle Aged; Patient Education as Topic*; Quality of Life; Self Care*
  8. Ismail SF, Harun D, Rahim NDA, Mohd Rasdi HF, Ker KJ
    Aust Occup Ther J, 2025 Feb;72(1):e13010.
    PMID: 39821092 DOI: 10.1111/1440-1630.13010
    INTRODUCTION: A Modified Occupational Questionnaire (MOQ) is a self-report questionnaire that records the occupational engagement of an individual hourly, focusing on activity category, reason for doing the activity, value to self, and value to society. Understanding the patterns of occupational engagement and meaningful time use in older persons is crucial for predicting physical and psychological health and establishing occupation-based interventions to support healthy aging. However, the MOQ, originally developed in an English-speaking country and may potentially be less suitable for Malaysian older persons, who are predominantly Malay-speaking and have distinct cultural adaptations. This study aimed to translate the MOQ from English to Bahasa Melayu and evaluate its validity and reliability among older persons in Malaysia.

    METHODS: The translation, validation, and reliability assessment process followed guidelines from the World Health Organization, with adaptations from Sousa and Rojjanasrirat's recommendations. The process encompassed five steps: forward translation, harmonisation of the forward translation, backward translation, pretesting and cognitive interviews, and psychometric testing to assess the validity and reliability.

    RESULTS: The Malay version of the MOQ (MOQ-M) demonstrated excellent item-level face validity index (I-FVI) and average scale-level face validity index (S-FVI/Ave), both scoring a perfect 1.00. Additionally, it exhibited excellent item-level content validity index (I-CVI) and average scale-level content validity (S-CVI/Ave), with scores ranging from 0.90 to 1.00 and 0.99 to 1.00, respectively. Internal consistency, measured using Cronbach's alpha, surpassed the minimum threshold for good reliability, with all three average scales in MOQ-M scoring between 0.84 and 0.99. Weighted average Cohen's Kappa coefficient revealed substantial agreement in the test-retest reliability across the three scales.

    CONCLUSION: The MOQ-M is a valid and reliable instrument for assessing meaningful time use among Malay-speaking older persons in Malaysia.

    CONSUMER AND COMMUNITY INVOLVEMENT: There was no consumer and community involvement in this study.

    PLAIN LANGUAGE SUMMARY: This study aimed to translate the Modified Occupational Questionnaire (MOQ) into Bahasa Melayu (MOQ-M). The MOQ is a tool used by occupational therapists to understand how unemployed people spend their time and the reasons they are doing it. The translation was necessary because the original version of the MOQ was developed in an English-speaking country and might not be suitable for the Malay-speaking population in Malaysia due to cultural differences. A careful process was followed to make sure the translation was accurate and reliable. The translated version, MOQ-M, was then tested with older persons, most of whom were unemployed, to learn about their daily activities and the reasons for doing them. Understanding their daily routines is important for predicting their health and well-being. The results showed that the MOQ-M worked well, providing useful and consistent information. This means that the tool is reliable and can be trusted to measure how older persons spend their time and what activities are important to them. With this information, programmes can be created to better support older persons, helping them live healthier and happier lives as they age. However, it is important to note that most of the study participants were Malay, so this may not fully represent the different cultural groups in Malaysia.

    MeSH terms: Activities of Daily Living; Aged; Aged, 80 and over; Female; Humans; Malaysia; Male; Middle Aged; Surveys and Questionnaires/standards; Translating; Translations; Reproducibility of Results
  9. Vafa S, Thanaraju A, Chan JK, Harris HA, Chan XW, Todi K, et al.
    Appl Psychol Health Well Being, 2025 Feb;17(1):e12651.
    PMID: 39822168 DOI: 10.1111/aphw.12651
    Physical and cognitive interventions are deemed the primary methods of improving cognitive functioning in healthy older adults. However, the effectiveness of these interventions is still debated. This systematic review, synthesised findings from the literature on four different types of interventions: physical activities, cognitive training, cognitive stimulation and a combined intervention. We searched six databases for each intervention category. Findings demonstrated that 65% of the studies across all intervention groups reported improvement in the experimental group following the intervention. Furthermore, memory, executive and global cognitive functions were the most reported improvements post-intervention. Additionally, participants with higher education benefited more from cognitive training (an intervention that targets a specific cognitive domain), while those with lower education gained more following cognitive stimulation (an intervention that targets general domains). Lastly, in sub-types of physical activity, cognitive stimulation and combined category, longer durations (more than 20 sessions) were associated with significant cognitive improvements. Conversely, in cognitive training, having less than 20 sessions led to significant results. Findings indicated an interaction of education and intervention duration with significant outcomes post-intervention. In conclusion, this review demonstrated the importance of intervention type, duration and education in understanding cognitive improvement post-intervention.
    MeSH terms: Aged; Humans; Exercise*; Cognitive Therapy/methods
  10. Ren Q, Lim YY, Teo CH
    3 Biotech, 2025 Feb;15(2):41.
    PMID: 39822754 DOI: 10.1007/s13205-025-04213-9
    Orphan genes (OGs), also known as lineage-specific genes, are species-specific genes that play a crucial role in species-specific adaptations to various stresses. Although OGs have been identified in several plant species, there is no information on OGs in banana genomes. This study aimed to systematically identify OGs in twelve banana (sub)species using comparative genomics. The results showed that OG content varied widely among these (sub)species, from 0.4% in Musa itinerans to 7.3% in Ensete glaucum. Genetic structure analysis showed that banana OGs have significantly shorter protein lengths, smaller molecular weight, fewer exons, and shorter exon lengths than non-orphan genes (NOGs). Subcellular localization predictions showed that banana OGs are mainly found in the chloroplast, nucleus, and cytosol, and are evenly distributed across chromosomes. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses suggested that OGs may be involved in cellular processes, metabolic processes, and molecular transport. The transcriptome analysis of 9 AAA cultivars against 4 M. acuminata subspecies genomes showed the OGs content. Analysis of gene expression in M. acuminata subsp. malaccensis showed 75 differentially expressed (DE) OGs in response to abiotic stresses and 46 DE OGs related to biotic stresses, indicating that these OGs might play important roles in response to abiotic and biotic stresses. This study provides a foundation for further in-depth research into the functions of OGs in bananas.

    SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s13205-025-04213-9.

  11. Liu H, Lbrahim H, Song M
    PLoS One, 2025;20(1):e0314589.
    PMID: 39823423 DOI: 10.1371/journal.pone.0314589
    This study investigates the impact of low-carbon economic policies on Corporate Environmental Responsibility (CER) in Chinese A-share listed companies, with a particular focus on the role of financing constraints as a mediating factor. Despite a decrease in environmental pollution incidents in 2022, the economic and social impacts of such incidents remain significant, highlighting the need for stronger environmental governance. Building upon previous research, this study utilizes data from the Shanghai and Shenzhen stock exchanges (2010-2020) and employs a Difference-in-Differences (DID) model to assess the effects of low-carbon economic policies introduced in 2016 on CER. The findings reveal that these policies positively influence CER and that financial constraints act as a mediator. The study finds how low-carbon policies indirectly promote environmental commitments by alleviating financial barriers. The research provides valuable insights for policy formulation, advocating for intensified reforms on the financial supply side to foster a sustainable economic framework. Additionally, it underscores the importance of implementing robust low-carbon policies to elevate corporate environmental responsibility. However, the study also notes limitations related to data scope and potential external factors influencing the results. These findings contribute to the broader discourse on sustainable development, offering a blueprint for harmonizing economic growth with environmental preservation and informing future research in this area.
    MeSH terms: Carbon; China; Environmental Pollution/economics; Environmental Pollution/prevention & control; Humans; Economic Development
  12. Reiss-Woolever VJ, Wakhid W, Ikhsan M, Caliman JP, Naim M, Azmi EN, et al.
    PLoS One, 2025;20(1):e0304837.
    PMID: 39823441 DOI: 10.1371/journal.pone.0304837
    Smallholder farmers produce over 40% of global palm oil, the world's most traded and controversial vegetable oil. Awareness of the effects of palm oil production on ecosystems and human communities has increased drastically in recent years, with ever louder calls for the private and public sector to develop programs to support sustainable cultivation by smallholder farmers. To effectively influence smallholder practices and ensure positive social outcomes, such schemes must consider the variety in perspectives of farmers and align with their priorities. We conducted social surveys on smallholder farmers in Indonesia and Malaysia with varying degrees of participation in programs that offer advice and support with plantation management ("management-assistance programs") led by an industrial palm oil producer in Indonesia and a conservation-focused NGO in Malaysia. We surveyed farmers on their demographics, attitudes, and management decisions. Our analyses act as case studies to investigate the similarities and differences between smallholder palm oil producers involved in different schemes, allowing us to determine the alignment between the intentions of partnership programs and the current realities of smallholder plantations. The relationship between heterogeneity of social factors and management decisions and degree of program involvement differed across different groups and region: Indonesian smallholders most closely partnered with the private sector were the most varied in socio-demographics and attitudes but showed little variation in management inputs, while Malaysian smallholders most closely partnered with an NGO were the most heterogenous across all survey sections. Specifically, Indonesian farmers partnered with the private sector used less herbicide, more fertilizer, and had higher yield and total household income than farmers completely uninvolved with management assistance programs. In Malaysia, farmers partnered with an NGO also had higher yield and fertilizer application than independent farmers, however they used significantly more herbicide and had lower total household income. Our findings demonstrate the wide variety of smallholder farmers in both regions, directly opposing a 'one-size-fits-all' approach to sustainability. The wide variety of existing management practices also provides a potentially valuable natural experiment to identify high-yield, environmentally-friendly management approaches. When taken in context, our findings may inform the interventions of management-assistance programs, ensuring they are approaching the most relevant farmer groups in the most effective way.
    MeSH terms: Agriculture/methods; Attitude; Conservation of Natural Resources/methods; Female; Humans; Indonesia; Malaysia; Male; Surveys and Questionnaires; Private Sector
  13. Capelle DP, Sabirin W, Zulhairy-Liong NA, Edgar S, Goh KJ, Ahmad-Annuar A, et al.
    PMID: 39363643 DOI: 10.1080/21678421.2024.2410280
    OBJECTIVE: To apply the multistep model of pathogenesis in amyotrophic lateral sclerosis (ALS) to data from a multiethnic Malaysian registry.

    METHODS: Clinical data, including age at symptom onset, was collected from 289 patients who presented to our multidisciplinary clinic from 2016 until 2024. A least squares linear regression model was constructed from the logarithm of approximated incidence and the logarithm of age. Population incidence was approximated by adjusting the absolute numbers of patients in 5 year groups by the size of the general population in the respective age group.

    RESULTS: A linear relationship between log of incidence versus log of age was observed, with a slope of 4.57 (95% CI, 3.3-5.8) and an r2 value of 0.93, suggesting a 6-step process.

    CONCLUSION: Progression toward symptom onset in Malaysian ALS patients appears consistent with a multistep model of disease as observed in other cohorts.

    MeSH terms: Adult; Aged; Female; Humans; Malaysia/epidemiology; Male; Middle Aged; Registries*; Incidence; Age of Onset; Disease Progression*
  14. Ibrahim SH, Mohd FN, Yusof MYPM, Chandra LA, Ahmi A, Ahmad MS
    Spec Care Dentist, 2025;45(1):1-13.
    PMID: 39817604 DOI: 10.1111/scd.13102
    AIMS: Dental anxiety (DA) is reported among the general population, including individuals with learning disabilities (LDs). In many studies, nonpharmacological interventions have been applied to significantly improve DA among patients. This study aimed to examine publication trends and patterns in both populations to identify the current research trends and literature gaps.

    METHODS: A descriptive analysis of bibliographic data and network visualization were conducted using Microsoft Excel and VosViewer, focusing on literature published in the Scopus database between 1994 and 2023 and using "title" as the search field.

    RESULTS: For the study period, the following were identified: 1429 authors, 5858 total citations, and an h-index of 36. From a total of 2058 publications discovered, 363 publications were included in the study. Of these, only 11 articles addressed nonpharmacological management for people with LDs. In terms of the research country, the United Kingdom was the leading contributor, with 56 publications. Ad de Jongh was the most productive author, with 13 publications and 447 citations. The most highly cited paper was Lehrner et al. (2005), with 312 citations.

    CONCLUSION: This study determined the number of publications related to nonpharmacological management of DA over three decades. The number of studies involving people with LDs was lower than that involving the general population, suggesting future research should occur in this area.

    MeSH terms: Humans; Bibliometrics*; Dental Anxiety*
  15. Deng J, Naresh Sethi NSA, Ahmad Kamar A, Saaid R, Loo CK, Mattar CNZ, et al.
    Prenat Diagn, 2025 Mar;45(3):374-386.
    PMID: 39817730 DOI: 10.1002/pd.6748
    OBJECTIVE: The first objective is to develop a nuchal thickness reference chart. The second objective is to compare rule-based algorithms and machine learning models in predicting small-for-gestational-age infants.

    METHOD: This retrospective study involved singleton pregnancies at University Malaya Medical Centre, Malaysia, developed a nuchal thickness chart and evaluated its predictive value for small-for-gestational-age using Malaysian and Singapore cohorts. Predictive performance using conjunctive (AND)/disjunctive (OR) rule-based algorithms was assessed. Seven machine learning models were trained on Malaysia data and evaluated on both Malaysia and Singapore cohorts.

    RESULTS: 5519 samples were collected from the University Malaya Medical Centre. Small-for-gestational-age infants exhibit significantly lower nuchal thickness (small-for-gestational-age: 4.57 [1.04] mm, appropriate-for-gestational-age: 4.86 [1.06] mm, p 

    MeSH terms: Machine Learning*; Adult; Female; Gestational Age; Humans; Infant, Newborn; Infant, Small for Gestational Age*; Malaysia; Predictive Value of Tests; Pregnancy; Retrospective Studies; Singapore; Fetal Weight*
  16. Ewane EB, Selvam PP, AlMealla R, Watt MS, Arachchige PSP, Bomfim B, et al.
    J Environ Manage, 2025 Feb;374:124074.
    PMID: 39818073 DOI: 10.1016/j.jenvman.2025.124074
    Mangrove-based carbon market projects (MbCMP) aim to conserve, protect and restore mangrove habitats in order to generate high quality blue carbon credits via a crediting program, as a contribution to climate change mitigation/adaptation, biodiversity conservation, ecosystem services provision and local socio-economic development. The blue carbon credits generated are transferable, verifiable and sold through carbon markets to earn additional income for governments and local communities. The main aim of the paper is to provide important considerations for pre-field planning, that is, how challenges associated with fieldwork, project implementation, and monitoring reporting and verification (MRV) can be addressed with proper pre-field planning. Herein, we present a comprehensive list of some major concerns, which should be addressed by MbCMP developers and other stakeholders prior to embarking on fieldwork and project implementation. Moreover, we propose a methodological protocol and guideline principles for enhancing field data collection, project implementation and MRV planning. This should help address the major concerns that contribute to inaccurate and controversial baselines and project scenarios around MbCMP. By addressing these major concerns and adhering to the proposed methodological protocol and guideline principles during pre-field preparation planning, MbCMP developers and the respective MbCMP stakeholders can effectively improve the accuracy and efficiency of field data collection to estimate robust and reliable baseline and project scenarios. This will go a long way to building public trust, support and engagement in MbCMP to achieve the desired positive ecological, economic and social outcomes.
    MeSH terms: Carbon*; Conservation of Natural Resources*; Ecosystem; Biodiversity; Wetlands; Climate Change*
  17. Lasso G, Grodus M, Valencia E, DeJesus V, Liang E, Delwel I, et al.
    Cell Host Microbe, 2025 Feb 12;33(2):294-313.e11.
    PMID: 39818205 DOI: 10.1016/j.chom.2024.12.016
    Evidence suggests that bats are important hosts of filoviruses, yet the specific species involved remain largely unidentified. Niemann-Pick C1 (NPC1) is an essential entry receptor, with amino acid variations influencing viral susceptibility and species-specific tropism. Herein, we conducted combinatorial binding studies with seven filovirus glycoproteins (GPs) and NPC1 orthologs from 81 bat species. We found that GP-NPC1 binding correlated poorly with phylogeny. By integrating binding assays with machine learning, we identified genetic factors influencing virus-receptor-binding and predicted GP-NPC1-binding avidity for additional filoviruses and bats. Moreover, combining receptor-binding avidities with bat geographic distribution and the locations of previous Ebola outbreaks allowed us to rank bats by their potential as Ebola virus hosts. This study represents a comprehensive investigation of filovirus-receptor binding in bats (1,484 GP-NPC1 pairs, 11 filoviruses, and 135 bats) and describes a multidisciplinary approach to predict susceptible species and guide filovirus host surveillance.
    MeSH terms: Machine Learning*; Animals; Glycoproteins/genetics; Glycoproteins/metabolism; Humans; Phylogeny; Protein Binding; Filoviridae Infections/metabolism; Filoviridae Infections/virology; Ebolavirus/genetics; Ebolavirus/metabolism; Ebolavirus/physiology
  18. Suwendar S, Jantan I, Fakih TM, Priani SE, Mulyanti D, Patricia VM, et al.
    J Biomol Struct Dyn, 2025 Feb;43(3):1539-1551.
    PMID: 38084758 DOI: 10.1080/07391102.2023.2292294
    This research is part of the research umbrella regarding the use of watery rose apple leaf [Eugenia aqueum (Burm. F) Alston] in the health sector. Infectious diseases of worms, are still a health problem with a high burden based on the incidence and mortality rates in Southeast Asian countries. Therefore, we tried to create a 3D structure of vital receptors of worms including Nicotinic Cholinergic Receptor (NCR), Acetylcholinesterase Enzymes (AE), and Phosphorylase Enzymes (PE) using homology modeling method through the SWISS-MODEL webserver, then conducted a molecular dynamics simulations using active metabolite of watery rose apple leaf i.e. 2',4'‑dihydroxy‑6'‑methoxy‑3',5'‑dimethylchalcone which was then compared with Piperazine Citrate and Pyrantel Pamoate which are anthelmintic drugs on the market. Based on the results of the study, the structure of the three receptors with high resolution was successfully obtained which was characterized by a good Ramachandran value, which was above 90%. Then the ligand structure was successfully modeled in a three-dimensional model and optimized geometrically using GaussView 5.0.8 and Gaussian09 software. In further, Root Mean Square Deviation (RMSD) and Root Mean Square Fluctuation (RMSF) analysis of molecular dynamics simulations ligand against the three receptors, compound 2',4'‑dihydroxy‑6'‑methoxy‑3',5'‑dimethylchalcone showed better stability than Piperazine Citrate and Pyrantel Pamoate at 50 ns simulation, although from the calculation of binding free energy Molecular Mechanics Poisson-Boltzmann Surface Area (MM/PBSA) is not better than the two comparisons. It can be concluded that the compound 2',4'‑dihydroxy‑6'‑methoxy‑3',5'‑dimethylchalcone from watery rose apple leaf showed potential as anthelmintic candidates.Communicated by Ramaswamy H. Sarma.
    MeSH terms: Amino Acid Sequence; Animals; Ligands; Plant Extracts/pharmacology; Plant Extracts/chemistry; Protein Binding; Malus; Molecular Dynamics Simulation*; Molecular Docking Simulation*
  19. Getchell M, Wulandari S, de Alwis R, Agoramurthy S, Khoo YK, Mak TM, et al.
    Nat Microbiol, 2025 Jan;10(1):258.
    PMID: 39414934 DOI: 10.1038/s41564-024-01848-x
  20. He F, Aebersold R, Baker MS, Bian X, Bo X, Chan DW, et al.
    Nature, 2025 Jan;637(8046):E22.
    PMID: 39715925 DOI: 10.1038/s41586-024-08555-x
External Links