Browse publications by year: 2025

  1. Atikah A, Suzana M, Wan Haslina WAH, Norshamsiah MD, Mushawiahti M, Birinder KSS, et al.
    Cont Lens Anterior Eye, 2025 Apr;48(2):102346.
    PMID: 39676005 DOI: 10.1016/j.clae.2024.102346
    PURPOSE: To assess the short-term effects of topical insulin (TI) as compared to topical Systane UltraTM (Alcon, USA) as artificial tears (AT) and topical normal saline (NS) on tear inflammatory mediators; interleukin 1-alpha (IL-1a), interleukin 6 (IL-6) and matrix metalloproteinase 9 (MMP-9) and clinical dry eye parameters in diabetics with dry eye disease (DDED).

    METHODS: In this randomized, multi-arm parallel single-centre trial, 73 diabetics with dry eye disease (30 female and 43 male) aged 18 to 50 years were recruited. They were randomly assigned to four weeks of treatment of either TI 0.5 unit/drop, AT or NS four times per day. Tear sample analysis and clinical parameters of dry eye (Ocular Surface Disease Index (OSDI), tear break-up time (TBUT), corneal Oxford score and Schirmers test) were evaluated at baseline and four weeks later. Objective clinical parameters for corneal Oxford score and tear break-up time were assessed by two masked investigators.

    RESULTS: Significant reduction was seen in all inflammatory biomarkers (IL-1a, IL-6 and MMP-9) in all treatment groups at 4 weeks. In addition, inflammatory biomarkers demonstrated a significant reduction with TI (p 

    MeSH terms: Administration, Topical; Adolescent; Adult; Female; Humans; Hypoglycemic Agents/administration & dosage; Male; Middle Aged; Ophthalmic Solutions*; Sodium Chloride/administration & dosage; Biomarkers/metabolism; Treatment Outcome; Inflammation Mediators/metabolism; Matrix Metalloproteinase 9/metabolism; Young Adult; Administration, Ophthalmic
  2. Dou X, Saalah S, Chiam CK, Xie J, Sipaut CS
    J Mater Chem B, 2025 Jan 22;13(4):1180-1194.
    PMID: 39679535 DOI: 10.1039/d4tb02207f
    Ultrasmall metal nanoclusters (NCs, <2 nm) have emerged as a novel class of luminescent probes due to their atomically precise size and tailored physicochemical properties. The rapid advancements in the design and utilization of metal NC-based luminescent probes are facilitated by the atomic-level manipulation of metal NCs. This review article explores (i) the engineering of metal NCs' functions for bioimaging applications, and (ii) the diverse uses of metal NCs in bioimaging. We begin by presenting an overview of the engineering functions of metal NCs as luminescent probes for bioimaging applications, highlighting key strategies for enhancing NCs' luminescence, biocompatibility and targeting capabilities towards biological specimens. Our discussion then centers on the bioimaging applications of metal NCs in subcellular organelles, individual cells, tissues, and entire organs. Finally, we offer a perspective on the challenges and potential developments in the future use of metal NCs for bioimaging applications.
    MeSH terms: Animals; Fluorescent Dyes/chemical synthesis; Fluorescent Dyes/chemistry; Humans; Particle Size; Luminescent Agents/chemical synthesis; Luminescent Agents/chemistry; Optical Imaging
  3. Fuad ASM, Arzmi MH, Bakar ME, Taib IS, Khuda F, Nasruddin NS
    J Ayurveda Integr Med, 2025;16(1):101037.
    PMID: 39681048 DOI: 10.1016/j.jaim.2024.101037
    BACKGROUND: The combination of S. salivarius K12 and M. acuminata are being used as synbiotic, but its safety evaulation is required.

    OBJECTIVE: This study aimed to determine the LD50 of synbiotic containing probiotic Streptococcus salivarius K12 and prebiotic Musa acuminata peel extract.

    MATERIALS AND METHODS: The determination of LD50 is done according to the Acute Oral Toxicity test No. 425 (AOT425). For limit test, five female Sprague Dawley rats were given a limit dose of 2000 mg/kg of the synbiotic mixture once orally, and observed for 12 days. For subacute toxicity test, twenty female Sprague Dawley rats were randomised into 4 groups (n = 5). Control group received saline, others received synbiotic mixture at doses 175 mg/kg, 550 mg/kg, and 2000 mg/kg, respectively, and observed for 14 days. Animals were euthanised on day-15, blood was collected, and subjected to haematological and biochemical analyses. Kidney and liver were preserved for histopathological examination.

    RESULT: No significant changes on the average body weight of the animals throughout the study. Haematological parameters and biochemical analysis do not depict any changes related to acute toxicity. Histopathology analysis depicted mild changes on kidney and liver.

    CONCLUSION: Based on the data, the LD50 of the synbiotic formulation is higher than 2000 mg/kb, with no sign of acute toxicity observed on all parameters.

  4. Clyde DR, Adib R, Baig S, Bhasker AG, Byrne J, Cameron D, et al.
    Clin Obes, 2025 Apr;15(2):e12722.
    PMID: 39673462 DOI: 10.1111/cob.12722
    Global obesity rates have risen dramatically, now exceeding deaths from starvation. Metabolic and bariatric surgery (MBS), initially for severe obesity (BMI ≥35 kg/m2), is performed globally over 500 000 times annually, offering significant metabolic benefits beyond weight loss. However, varying eligibility criteria globally impact patient care and healthcare resources. Updated in 2022, ASMBS and IFSO guidelines aim to standardise MBS indications, reflecting current understanding and emphasising comprehensive preoperative assessments. Yet, clinical variability persists, necessitating consensus-based recommendations. This modified Delphi study engaged 45 global experts to establish consensus on perioperative management in MBS. Experts selected from bariatric societies possessed expertise in MBS and participated in a two-round Delphi protocol. Consensus was achieved on 90 of 169 statements (53.3%), encompassing multidisciplinary team composition, patient selection criteria, preoperative testing, and referral pathways. The agreement highlighted the critical role of comprehensive preoperative assessments and the integration of healthcare professionals in MBS. These findings offer essential insights to standardise perioperative practices and advocate for evidence-based guidelines in MBS globally. The study underscores the need for unified protocols to optimise outcomes and guide future research in MBS.
    MeSH terms: Delphi Technique*; Female; Humans; Male; Obesity, Morbid/surgery; Patient Selection; Consensus*
  5. Cai R, Tan CP, Lai OM, Dang Y, Liu A, Choeng LZ, et al.
    Food Chem, 2025 Mar 15;468:142408.
    PMID: 39674013 DOI: 10.1016/j.foodchem.2024.142408
    Casein (CN) is a common allergen that is challenging to avoid in modern foods. The effect of cold argon plasma (CAP) on reducing CN antigenicity was investigated, focusing on alterations in epitope structure and sequence. CAP mainly contains hydroxyl radicals (∙OH). After a 12-min CAP treatment, the result of ELISA demonstrated an 80.46 % reduction in antigenicity. Transmission electron microscopy and electrophoresis revealed that certain CN aggregated, while multispectral analysis indicated that part of CN was fragmented into smaller peptides. The predictive 3D model suggested the disruption of linear epitopes located in the α-helix region might contribute to the reduced allergenicity. The peptide sequences were compared to the linear epitopes predicted by immunoinformatics approaches, revealing some reduction or breakage of key allergic sequences. Meanwhile, amino acids with aromatic side chains and hydrophobic groups were susceptible to CAP-induced modifications. This investigation demonstrated CAP could be beneficial for processing hypoallergenic foods.
    MeSH terms: Amino Acid Sequence; Animals; Epitopes/immunology; Epitopes/chemistry; Food Hypersensitivity/immunology; Humans; Peptides/immunology; Peptides/chemistry
  6. Khalid AA, Abd Razak NI, Anuar ST, Ibrahim YS, Rusli MU, Jaafar M
    Mar Pollut Bull, 2025 Feb;211:117412.
    PMID: 39674038 DOI: 10.1016/j.marpolbul.2024.117412
    This study presents the first evidence of microplastics in natural sea turtle nests at Chagar Hutang Turtle Sanctuary (CHTS) on Redang Island, a crucial habitat for green turtles. Microplastics were detected in all studied turtle nests (0-70 cm depth), with a total abundance of 12,270 microplastic items per kg dry weight of sand. Fibers (80.7 %) were the most prevalent shape, primarily black (37.9 %), with a high proportion of small-sized particles (<300-1000 μm), indicating prolonged accumulation and environmental degradation. These microplastics, primarily polyethylene terephthalate (PET) and polyethylene (PE), are likely from fishing nets, food packaging, and textiles. Despite the sanctuary's remote location, it is heavily impacted by microplastics carried by tides and wind, raising concerns about their potential effects on turtle egg incubation. The findings offer critical insights into the impact of microplastics on turtle habitats and underscore the urgent need for conservation efforts to protect green sea turtles in Malaysia.
    MeSH terms: Animals; Environmental Monitoring*; Malaysia; Turtles*; Ecosystem; Islands
  7. Wang J, Chang B
    Acta Psychol (Amst), 2025 Feb;252:104668.
    PMID: 39674078 DOI: 10.1016/j.actpsy.2024.104668
    Using Latent Profile Analysis, this study explored the connections between different marginalization profiles and perceptions of decent work among rural primary school teachers in China. Analyzing data from 443 teachers across seven rural primary schools in Fujian Province, we discerned three unique marginalization profiles: Robust Adapters (n = 182, 41 %), Challenge Facers (n = 210, 47 %), and Conditioned Survivors (n = 51, 12 %). These profiles were examined for their links to decent work dimensions. Findings indicated that the Robust Adapters significantly outperformed both the Challenge Facers and Conditioned Survivors across all five dimensions of decent work. In contrast, there were no significant differences between the performances of the Challenge Facers and Conditioned Survivors in these dimensions, which indicated that more marginalization was not inevitably linked to less decent work. The study concluded by discussing the implications of these findings and the directions of future research.
    MeSH terms: Adult; China; Female; Humans; Male; Middle Aged; Social Marginalization
  8. Benhadji N, Kurniawan SB, Imron MF
    Sci Total Environ, 2025 Jan 01;958:178057.
    PMID: 39674161 DOI: 10.1016/j.scitotenv.2024.178057
    Heavy metal and microplastic pollutions are prevalent in freshwater ecosystems, with many freshwater bodies being contaminated by one or both of these pollutants. Recent studies reported extreme detections of Cd, Pb and Zn, high concentrations of Cr, Pb and Cu and microplastics acting as vectors of pollutants, including heavy metals. Mayflies can serve as bioindicators of heavy metal contamination in freshwater ecosystems because changes in their community structure, physiology, and behaviour can reflect and help predict the concentrations of metals in these environments. This review discusses the ecological alterations induced by tissue metal concentration in mayflies and other macroinvertebrates. As sensitive taxa to heavy metal contamination, mayflies can reflect the impacts of this pollution through their ethology and relationship to the substrate, highlighting issues such as eutrophication, alterations in community structure, inhibitory effects and sediment toxicity. Mayflies are also highly affected by microplastic exposure, which leads to ingestion, bioaccumulation, biomagnification, habitat and community alteration, behavioural changes, physiology alteration and toxicity. Mayflies bioindication metrics for assessing the impact of heavy metals and microplastics include the examination of community alteration, functional feeding behaviour, molecular structure, dietary and toxicity impacts, bioaccumulation and biomagnification and biomarkers. Current challenges for the utilization of mayflies as bioindicators include temporal variations in sensitivity, lack of universally recognised protocols and need for standardised protocols for microplastic analysis. Additionally, the applicability of mayflies as bioindicators may vary across different ecosystems, emphasising the need for selecting suitable indicators that align with the unique characteristics of the ecosystem.
    MeSH terms: Animals; Water Pollutants, Chemical*; Ecosystem; Ephemeroptera*
  9. Gries JJ, Lazarus JV, Brennan PN, Siddiqui MS, Targher G, Lang CC, et al.
    Lancet Gastroenterol Hepatol, 2025 Jan;10(1):82-94.
    PMID: 39674228 DOI: 10.1016/S2468-1253(24)00310-8
    Metabolic dysfunction-associated steatotic liver disease (MASLD) has emerged as a public health threat as it affects approximately 38% of the adult population worldwide, with its prevalence rising in step with that of obesity and type 2 diabetes. Beyond the implications of MASLD for liver health, it is also associated with cardiovascular and vascular dysfunction. Although the many shared risk factors and common metabolic milieu might indicate that cardiovascular disease and MASLD are discrete outcomes from common systemic pathogeneses, a growing body of evidence has identified a potential causal relationship between MASLD and coronary artery disease, which is the leading cause of morbidity and mortality in people with MASLD and all-cause mortality worldwide. This Review takes an interdisciplinary approach, drawing on hepatology, cardiology, endocrinology, and metabolic and internal medicine specialists to help to delineate the intricate interplay between MASLD and coronary artery disease. It sheds light on novel opportunities for targeted interventions and personalised management strategies.
    MeSH terms: Fatty Liver/complications; Fatty Liver/metabolism; Fatty Liver/therapy; Humans; Risk Factors; Non-alcoholic Fatty Liver Disease/complications; Non-alcoholic Fatty Liver Disease/metabolism; Non-alcoholic Fatty Liver Disease/therapy
  10. Gauhar V, Somani BK, Seitz C, Castellani D, Tefik T, Persaud S, et al.
    Urology, 2025 Mar;197:42-50.
    PMID: 39674378 DOI: 10.1016/j.urology.2024.12.009
    OBJECTIVE: To assess urologists' perceptions and current practices of using suction-based techniques and technologies in percutaneous nephrolithotomy (PCNL) and retrograde intrarenal surgery (RIRS) for kidney stones.

    MATERIALS AND METHODS: A customized web-based 28-item questionnaire was created. All participation was voluntary. The survey was hosted on Google Forms between November 15, 2023 and November 25, 2023. Descriptive statistics were applied to demographic details and categorical responses.

    RESULTS: Six hundred thirty-two responses were received. 56.5% were from Asia. Around 41.6% of respondents underwent an endourology fellowship. Personal experience of suction for PCNL was noted in 55.4% and 42.7% for RIRS. Exactly 34.9% believe that currently there is enough evidence that suction can improve stone-free rates and lower complications for both endourology surgeries. Around 55.69% believe that as evidence evolves, suction has the potential to be a game changer in endourology management of urolithiasis. Exactly 55.7% believe that the leading barrier to the adoption of suction in endourology is the lack of availability of technology. Other barriers include lack of procedural standardization (37.65%), lack of evidence (34.9%), lack of proper training (29.58%) and lack of advocation in the current guidelines (17.72%).

    CONCLUSION: Our survey shows that urologists are keen to adopt suction for PCNL and RIRS but the lack of definitive evidence, standardization, equipment availability and training preclude them from using so. More high-level evidence regarding the utility of suction in endourology is required for its routine adoption in clinical practice.

    MeSH terms: Adult; Attitude of Health Personnel*; Europe; Female; Humans; Kidney Calculi/surgery; Male; Practice Patterns, Physicians'/statistics & numerical data; Surveys and Questionnaires; Societies, Medical; Suction; Urologic Surgical Procedures; Urology*; Internationality; Urologists/statistics & numerical data
  11. Suhaimi A, Jawad AH, Yusoff MZM, Wilson LD, ALOthman ZA
    Int J Biol Macromol, 2025 Feb;288:138650.
    PMID: 39674466 DOI: 10.1016/j.ijbiomac.2024.138650
    A bio-composite material was developed that contains chitosan, food-grade algae, and zeolite for the removal of brilliant green (BG) dye. The synthesized bio-composite was dried via two different methods (air-drying; AD, and freeze-drying; FD). The physicochemical characterization of air-dried chitosan-algae-zeolite (Cs-Alg-Zl-AD) and freeze-dried chitosan-algae-zeolite (Cs-Alg-Zl-FD) were investigated by spectroscopy (FTIR, SEM-EDX, and XPS), diffraction (XRD), surface charge via pHpzc, specific surface area (SSA) and elemental analyses. The utilization of Box-Behnken Design (BBD) was intended to optimize the three input variables, which are adsorbent dosage, pH of medium, and contact time. The adsorption optimization process yielded optimal conditions, which were verified through a desirability test and implemented in batch-mode equilibrium experiments. The Cs-Alg-Zl-FD has a higher specific surface area (SSA = 3.29 m2/g) compared to Cs-Alg-Zl-AD (SSA = 1.79 m2/g). The Cs-Alg-Zl-FD shows greater adsorptive removal of BG (98.6 %) over Cs-Alg-Zl-AD (88.6 %), in parallel agreement with differences in the SSA. Moreover, the maximum BG dye adsorption capacities of Cs-Alg-Zl-FD (119.5 mg/g) and Cs-Alg-Zl-AD (108 mg/g) at pH = 8.1 and 25 °C. The Freundlich model fits best with Cs-Alg-Zl-AD while Langmuir and Temkin models account for the Cs-Alg-Zl-FD dye adsorption. The Cs-Alg-Zl-FD shows greater dye adsorption over four adsorption cycles, as compared with the Cs-Alg-Zl-AD.
    MeSH terms: Adsorption; Quaternary Ammonium Compounds; Coloring Agents/isolation & purification; Coloring Agents/chemistry; Freeze Drying*; Hydrogen-Ion Concentration; Kinetics; Water Purification/methods
  12. Yunesh K, Adlan ASA, Wong TY, Gan F, Hamdan M, Tan PC
    Am J Obstet Gynecol MFM, 2025 Feb;7(2):101579.
    PMID: 39674509 DOI: 10.1016/j.ajogmf.2024.101579
    BACKGROUND: Induction of labor after 1 previous cesarean delivery with an unripe cervix have an especially high risk for unplanned cesarean delivery and uterine rupture. Mechanical balloon ripening is often preferred over prostaglandin-based ripening during induction of labor after 1 previous cesarean delivery, because uterine rupture has been associated with prostaglandin use. The transcervical Foley balloon can be dislodged past the ripened cervix and be retained comfortably in the vagina during passive placement. Tugging of the Foley catheter can be a noninvasive and tolerated method for timelier retrieval of the retained balloon; expediting discovery of a ripened cervix would permit an earlier follow-on amniotomy and oxytocin infusion, thereby hastening birth.

    OBJECTIVE: This study aimed to evaluate the effect of tugging of the Foley balloon for 30 seconds every 3 hours during 12 hours of scheduled placement in comparison with standard care (no tugging) on the induction to birth interval and patient satisfaction.

    STUDY DESIGN: This randomized controlled trial, conducted at a university hospital in Malaysia, recruited participants from April 2023 to March 2024. Eligible participants admitted for Foley balloon induction of labor who had 1 previous cesarean were recruited. After balloon insertion, participants were randomized to tugging or standard care. Following balloon displacement, a vaginal examination was performed to check the cervix. The examination findings then dictated the follow-on management of induction of labor after 1 previous cesarean according to standard practice. The primary outcomes were the induction (Foley insertion) to delivery interval and participants' satisfaction with the birthing process. Secondary outcomes were largely derived from the core outcome set for trials on induction of labor. Based on a superiority hypothesis, it was calculated that a sample size of 126 would be required to detect a 4-hour reduction in the induction-to-delivery interval and a 1.5 point increase in the satisfaction score based on a 0 to 10 numerical rating scale with the level of significance set at 0.05, power at 80%, and a 10% drop out rate. Comparative analyses using an intention-to-treat approach were conducted using t tests, Mann-Whitney U tests, chi square tests, and Fisher exact tests as appropriate.

    RESULTS: A total of 126 participants were randomized with 63 in each trial arm. The induction to birth interval was (mean ± standard deviation) 29.7±9.6 hours for those in the 3-hourly tugging group and 29.8±9.1 hours for those under standard care (P=.950), and maternal satisfaction with the induction of labor after 1 previous cesarean delivery birth process, assessed using a 0 to 10 numerical rating scale, was (median [interquartile range]) 8 [7-9] and 8 [7-9] (P=.936), respectively. The cesarean delivery rate was 37 of 63 (59%) vs 41 of 63 (65%) (P=.238) for those in the tugging group vs standard care, and the main indication for unplanned cesarean delivery was failure to progress with an incidence of 24 of 37 (65%) and 24 of 41 (59%) (P=.914) for tugging vs standard care arms, respectively. Three-hourly tugging also did not shorten the interval from induction to balloon displacement, amniotomy, the start of oxytocin infusion, or the second stage of labor.

    CONCLUSION: Tugging the catheter every 3 hours during scheduled 12-hour Foley balloon placement for induction of labor after 1 previous cesarean delivery did not hasten birth or improve patient satisfaction when compared with standard care.

    MeSH terms: Adult; Catheterization/methods; Cesarean Section/methods; Cesarean Section/statistics & numerical data; Female; Humans; Malaysia; Pregnancy; Time Factors; Urinary Catheterization/methods; Patient Satisfaction*
  13. Chu C, Zhang Y, Yu R, Liu B, Wang B, Xu Z, et al.
    J Investig Med, 2025 Jan;73(1):54-66.
    PMID: 39324215 DOI: 10.1177/10815589241290199
    Cisplatin (DDP) resistance represents a pivotal contributing factor to chemotherapy failure and adverse patient outcomes in gastric cancer (GC). The objective of the present study was to investigate the roles and underlying mechanisms of myocyte enhancer factor 2A (MEF2A) in DDP resistance in GC. GC cell line AGS and MKN-45 cells were applied to construct DDP-resistant cells. CCK-8, colony formation, and flow cytometry methods were validated for determining the IC50 value of DDP and cell survival of GC cells. qRT-PCR and western blotting analysis quantified the molecular levels at mRNA and protein, respectively. Chromatin immunoprecipitation and dual-luciferase assays validated the molecular relationship between MEF2A and NF-κB inhibitor alpha (NFKBIA). Roles of MEF2A in in vivo were performed employing a xenograft model. The results showed that NFKBIA was greatly decreased in DDP-resistant AGS and MKN-45 cells compared to their respective parental cells. Increasing NFKBIA expression impaired the IC50 value of DDP and cell survival in DDP-resistant cells, while these alterations were rescued upon TNF-α treatment. Mechanistically, MEF2A acts as a transcriptional activator of NFKBIA, which led to the reduction of phosphorylation of p65 and cytoplasmic retention. Moreover, MEF2A overexpression promoted the sensitivity of GC cells to DDP and tumor growth, whereas these effects were partially reversed by NFKBIA silence. Collectively, MEF2A mitigated the DDP resistance in GC cells by modulatory actions on the NFKBIA/NF-κB signaling, shedding light on MEF2A/NFKBIA might be a promising intervention target for improving DDP resistance in GC.
    MeSH terms: Animals; Humans; Mice, Nude; Gene Expression Regulation, Neoplastic/drug effects; Xenograft Model Antitumor Assays; Cell Line, Tumor; Mice
  14. Mohd Isa D, Majid HA, Hairi FM, Harun Z, You YX, Abdul Manaf Z, et al.
    Asia Pac J Public Health, 2025 Jan;37(1):43-51.
    PMID: 39668727 DOI: 10.1177/10105395241304069
    Excessive salt or sodium intake is strongly linked to increased blood pressure, which is a major risk factor for cardiovascular diseases. This study aimed to qualitatively explore the views of key stakeholders on salt intake reduction and barriers as well as facilitators to reducing salt intake in Malaysian schools. The stakeholders in this study were school administrators, food operators, and consumers. Their views were determined using in-depth interviews and focus group discussions, and data collected were analyzed using inductive thematic analysis. Salient barriers identified in this study were a lack of knowledge pertaining to salt intake reduction and foods sold outside the school. Meanwhile, key facilitators of salt reduction included few strategies such as public health campaigns, priority toward salt reduction policies, and school-based education programs, alongside support from other relevant stakeholders. Overall, the barriers and facilitators signal the need for implementing salt reduction programs and the collaborative efforts of different stakeholders.
    MeSH terms: Diet, Sodium-Restricted; Female; Health Promotion; Humans; Health Knowledge, Attitudes, Practice; Malaysia; Male; Schools*; Focus Groups*; Sodium Chloride, Dietary*; Qualitative Research*
  15. Sujon MK, Samsurrijal SF, Baharin R, Isa NM, Zabidi MA, Mohd Noor SNF
    J Biomed Mater Res A, 2025 Jan;113(1):e37842.
    PMID: 39668803 DOI: 10.1002/jbm.a.37842
    Gamma irradiation is an effective technique for biocomposite films intended for application in tissue engineering (TE) to ensure sterility and patient safety prior to clinical applications. This study proposed a biocomposite film composed of natural polymer chitosan (CS) and synthetic polymer poly-Ɛ-caprolactone (PCL) reinforced with sol-gel-derived bioactive glass (BG) for potential application in TE. The BG/PCL/CS biocomposite film was sterilized using 25 kGy gamma rays, and subsequent changes in its characteristics were analyzed through mechanical and physical assessment, bioactivity evaluation via immersion in simulated body fluid (SBF) and biocompatibility examination using human primary dermal fibroblasts (HPDFs). Results indicated a homogeneous distribution of BG particles within the BG/PCL/CS polymer matrix which enhanced bioactivity, and the polymer blend provide a structurally stable film. Gamma irradiation induced an increase in the film's surface roughness due to photo-oxidative degradation; however, this did not adversely affect the integrity of glass particles and polymer chains. In vitro assessments demonstrated hydroxyapatite formation on the film's surface, suggesting bioactivity. Biocompatibility testing confirmed enhanced cell adhesion and proliferation. These multifunctional properties highlight the potential of the fabricated BG/PCL/CS biocomposite film for TE and regenerative medicine applications.
    MeSH terms: Cell Adhesion/drug effects; Gamma Rays*; Humans; Materials Testing*; Polyesters/chemistry; Chitosan/pharmacology; Chitosan/chemistry; Cell Proliferation/drug effects
  16. Kumar V, Jena D, Zahiruddin QS, Roopashree R, Kaur M, Srivastava M, et al.
    Int J Urol, 2025 Mar;32(3):277-284.
    PMID: 39670291 DOI: 10.1111/iju.15641
    OBJECTIVES: The objectives of this study were to analyze trends in prostate cancer incidence, incidence, mortality, and disability-adjusted life years (DALYs) from 1990 to 2021 via data from the Global Burden of Disease (GBD) study in South Asia. Additionally, the study projects future prostate cancer incidence rates up to 2031 to inform public health interventions in South Asia.

    METHODS: Data covering South Asian countries such as Bangladesh, Bhutan, India, Nepal, and Pakistan were obtained from the GBD 2021 portal. Age-standardized rates (ASRs) for prostate cancer metrics, including incidence (ASIR), prevalence (ASPR), mortality (ASMR), and DALYs (ASDR), were analyzed via joinpoint and ARIMA modeling techniques. Geographic variations in ASRs were mapped via QGIS software.

    RESULTS: The prostate cancer ASIR, ASPR, and ASDR significantly increased from 1990 to 2021, particularly among individuals aged 60-65 years. The highest incidence and mortality rates were observed in Pakistan. The total percentage change in incidence in India was the highest at 61%. Projections indicate a continued rise in prostate cancer incidence, with South Asia's ASIR expected to reach 9.34 per 100 000 by 2031.

    CONCLUSIONS: The growing burden of prostate cancer in South Asia highlights the need for enhanced screening programs, public awareness, and healthcare infrastructure improvements. Without intervention, the increasing incidence and mortality rates could strain healthcare resources, emphasizing the urgency of region-specific public health strategies.

    MeSH terms: Adult; Aged; Humans; Male; Middle Aged; Incidence; Prevalence
  17. Chundi R, G S, Basivi PK, Tippana A, Hulipalled VR, N P, et al.
    Comput Biol Med, 2025 Feb;185:109537.
    PMID: 39672014 DOI: 10.1016/j.compbiomed.2024.109537
    Early diagnosis and timely initiation of treatment plans for diabetes are crucial for ensuring individuals' well-being. Emerging technologies like artificial intelligence (AI) and computer vision are highly regarded for their ability to enhance the accessibility of large datasets for dynamic training and deliver efficient real-time intelligent technologies and predictable models. The application of AI and computer vision techniques to enhance the analysis of clinical data is referred to as eHealth solutions that employ advanced approaches to aid medical applications. This study examines several advancements and applications of machine learning, deep learning, and machine vision in global perception, with a focus on sustainability. This article discusses the significance of utilizing artificial intelligence and computer vision to detect diabetes, as it has the potential to significantly mitigate harm to human life. This paper provides several comments addressing challenges and recommendations for the use of this technology in the field of diabetes. This study explores the potential of employing Industry 4.0 technologies, including machine learning, deep learning, and computer vision robotics, as effective tools for effectively dealing with diabetes related aspects.
    MeSH terms: Machine Learning; Artificial Intelligence*; Humans; Telemedicine
  18. Hameed I, Khan DM, Ahmed SM, Aftab SS, Fazal H
    Comput Biol Med, 2025 Feb;185:109534.
    PMID: 39672015 DOI: 10.1016/j.compbiomed.2024.109534
    This systematic literature review explores the intersection of neuroscience and deep learning in the context of decoding motor imagery Electroencephalogram (EEG) signals to enhance the quality of life for individuals with motor disabilities. Currently, the most used non-invasive method for measuring brain activity is the EEG, due to its high temporal resolution, user-friendliness, and safety. A Brain Computer Interface (BCI) framework can be made using these signals which can provide a new communication channel to people that are suffering from motor disabilities or other neurological disorders. However, implementing EEG-based BCI systems in real-world scenarios for motor imagery recognition presents challenges, primarily due to the inherent variability among individuals and low signal-to-noise ratio (SNR) of EEG signals. To assist researchers in navigating this complex problem, a comprehensive review article is presented, summarizing the key findings from relevant studies since 2017. This review primarily focuses on the datasets, preprocessing methods, feature extraction techniques, and deep learning models employed by various researchers. This review aims to contribute valuable insights and serve as a resource for researchers, practitioners, and enthusiasts interested in the combination of neuroscience and deep learning, ultimately hoping to contribute to advancements that bridge the gap between the human mind and machine interfaces.
    MeSH terms: Machine Learning*; Brain/physiology; Humans; Imagination/physiology; Signal Processing, Computer-Assisted*; Brain-Computer Interfaces*
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