Browse publications by year: 2025

  1. Morthy P, Musarudin M, Shukor NSA, Said MA, Xianling D, Saripan MI
    Appl Radiat Isot, 2025 Jun;220:111786.
    PMID: 40121923 DOI: 10.1016/j.apradiso.2025.111786
    The present study was conducted to assess the accuracy of 177Lu quantification using Monte Carlo N-Particle Transport Code, Version 5 MCNP5. The developed code was verified against calibration factor (CF) measured experimentally. The CF for converting SPECT data into units of activity concentration was determined by modeling two phantom configurations: (1) a uniform 177Lu concentration of 5.3600 ± 0.0005 MBq/mL in 20 mL Petri dish, resulting in a CF1 of 12.5 ± 1.5 cps/MBq, and (2) a 65.4 mL radioactive sphere (5.0 cm diameter) within a non-radioactive background in a cylindrical Jaszczak phantom, yielding a CF2 of 16.0 ± 2.0 cps/MBq. The significant difference between CF1 and CF2 (21.26 %) highlights the impact of phantom size and geometry on the calibration process. The quantification error was evaluated using recovery coefficient (RC) of the spherical inserts in a NEMA phantom. The established CFs and RCs provide a reliable framework for accurate activity quantification in 177Lu SPECT imaging using the established MCNP5 code. Our findings suggest that MCNP5 simulations can effectively model the SPECT imaging process, accounting for factors such as photon attenuation and scatter, offering the potential for improved dosimetry calculations in radionuclide therapy.
  2. Mohammad SI, Vasudevan A, Hussein Alzewmel A, Rab SO, Ballal S, Kalia R, et al.
    Exp Cell Res, 2025 Mar 22;447(2):114508.
    PMID: 40122505 DOI: 10.1016/j.yexcr.2025.114508
    The tumor microenvironment (TME) 's primary constituents that promote cancer development are cancer-associated fibroblasts (CAFs). Metabolic remodeling has been shown to control CAF activity, particularly aberrant lipid metabolism. SCD1 can be thought of as the primary enzyme controlling the fluidity of lipid bilayers by gradually converting saturated fatty acids into monounsaturated fatty acids. Furthermore, its crucial function in the onset and spread of cancer is well acknowledged. Even with the increasing amount of research on changes in lipid metabolism, this problem remains a relatively understudied aspect of cancer research. Blocking several fatty acid synthesis-related enzymes highly expressed in cancerous cells inhibits cell division and encourages apoptosis. This is the situation with SCD1, whose overexpression has been linked to several changed tumors and cells. Both genetic and pharmacological silencing of SCD1 in cancer cells prevents glucose-mediated lipogenesis and tumor cell growth. However, its role in CAFs, hence, cancer biology, has been less studied. This study aimed to review the role of SCD1 in CAF biology, shedding light on their function in cancer cell biology.
  3. Bian C, Li D, Wang Y, He Z, Chen WT, Chong CM, et al.
    Sci Data, 2025 Mar 23;12(1):483.
    PMID: 40122884 DOI: 10.1038/s41597-025-04841-z
    Glass catfish (Kryptopterus vitreolus) is commonly distributed in several Asian countries, such as Thailand, Malaysia, and Indonesia. It is renowned for its near-transparent appearance, which has drawn considerable attention for biomedical research and the tropical ornamental fish industry. Here, we successfully constructed the first telomere-to-telomere (T2T) chromosome-scale genome assembly for glass catfish, by integration of PacBio HiFi, Nanopore ONT ultra-long, and Hi-C sequencing technologies. The haplotypic assembly covers approximately 687.7 Mb in length, featuring a high contig N50 of 21.3 Mb. This assembly was then anchored into 32 chromosomes, presenting a complete set of 64 telomeres and 32 centromeres. It was predicted with 252.4 Mb of repetitive sequences and annotated with a total of 24,696 protein-coding genes. Subsequent BUSCO analysis revealed high genome completeness (up to 96.4%). This high-quality T2T genome assembly not only provides a valuable genetic resource for investigating the molecular mechanisms underlying transparency, but also supports in-depth studies on functional genomics, genetic diversity, and selective breeding for this economically important fish species.
    MeSH terms: Animals; Chromosomes*; Genome*
  4. Masood N, Alkhadher SAA, Magam SM, Halimoon N, Alsukaibi A, Zakaria MP, et al.
    Environ Geochem Health, 2025 Mar 24;47(4):131.
    PMID: 40122936 DOI: 10.1007/s10653-025-02404-0
  5. Mallikarjun P, Thulasiraman SRG, Balachandran PK, Zainuri MAAM
    Sci Rep, 2025 Mar 23;15(1):10028.
    PMID: 40122953 DOI: 10.1038/s41598-025-94285-7
    This paper investigates the economic energy management of a wireless electric vehicle charging stations (EVCS) connected to hybrid renewable energy system comprising photovoltaic (PV), wind, battery storage, and the main grid. The study adopts an Improved Harris Hawk Optimization (IHHO) algorithm to optimize energy management and minimize operational costs under varying scenarios. Three distinct wireless EV charging load profiles are considered to evaluate the performance of the proposed optimization technique. Simulation results demonstrate that the IHHO algorithm achieves significant cost reductions and improves energy utilization efficiency compared to other state-of-the-art optimization algorithms such as Improved Quantum Particle Swarm Optimization (IQPSO), Honeybee Mating Optimization (HBMO), and Enhanced Exploratory Whale Optimization Algorithm (EEWOA). For scenarios with renewable energies, the IHHO algorithm reduced electricity costs by up to 36.41%, achieving a per-unit cost as low as 3.17 INR for the most demanding EV charging profile. Under scenarios of renewable generation disconnection, the IHHO algorithm maintained its superiority, reducing costs by up to 37.89% compared to unoptimized dispatch strategies. The integration of battery storage further enhanced the system's resilience and cost-effectiveness, particularly during periods of renewable unavailability. The IHHO algorithm's robust performance, reflected in its ability to handle dynamic and challenging operational conditions, demonstrates its potential for practical deployment in real-world wireless EVCS powered by hybrid renewable energy systems. The findings highlight the IHHO algorithm as a reliable and efficient tool for optimizing energy dispatch, promoting the integration of renewable energy, and supporting sustainable wireless EVCS infrastructure development. Simulation results demonstrate that IHHO outperforms all benchmark algorithms, achieving electricity cost reductions of up to 35.82% in EV Profile 3, with a minimum per-unit electricity cost of 3.11 INR/kWh across all scenarios. Specifically, IHHO achieved the lowest electricity cost of 6479.72 INR/day for EV Profile 1, 10,893.23 INR/day for EV Profile 2, and 20,821.63 INR/day for EV Profile 3, consistently outperforming IQPSO, HBMO, and EEWOA.
  6. Teoh KW, Baek Y, Ademi Z, Lee SWH
    Diabetes Obes Metab, 2025 Mar 24.
    PMID: 40123297 DOI: 10.1111/dom.16350
    AIMS: This study assessed the cost-effectiveness of a digital health-supported and community pharmacy-based lifestyle intervention (PRIME) programme for individuals with prediabetes in Malaysia over a 6-month period.

    MATERIALS AND METHODS: A trial-based cost-effectiveness study with a 6-month time horizon was conducted. Ninety-one participants (intervention, n = 46; usual care, n = 45) across 13 community pharmacies were included. The intervention group received in-depth counselling from pharmacists, in-app prediabetes education modules and peer support, while the usual care group received counselling based on pharmacists' usual practice. The primary outcome was quality-adjusted life years (QALY). Incremental cost-effectiveness ratios (ICER) per QALY gained of the intervention were compared with usual care from healthcare and societal perspectives. Non-parametric bootstrapping was used to examine uncertainty.

    RESULTS: At 6months, the QALY achieved was 0.467 (95% CI 0.456 to 0.479) in the intervention group and 0.466 (95% CI 0.451 to 0.482) in the usual care group, resulting in a net gain of 0.005 QALY (95% CI -0.017 to 0.026) in the intervention group. The incremental healthcare and societal costs were US$6.10 (95% CI $5.33 to $6.88) and $10.69 (95% CI $6.03 to $15.35), respectively. From a healthcare perspective, the ICER per QALY gained was $1354, with a probability of 69.2% being cost-effective, while the corresponding figures were $2371 and 67.7% from a societal perspective. Results were below the willingness-to-pay threshold at $11 845 and were robust to sensitivity analyses.

    CONCLUSION: A community pharmacy-based and digital health-supported lifestyle intervention to manage prediabetes may be cost-effective compared with usual care in Malaysia over a 6-month period.

  7. Azman KF, Zakaria R
    Curr Neuropharmacol, 2025;23(4):384-403.
    PMID: 40123457 DOI: 10.2174/1570159X22666240530105516
    Huntington's disease is a hereditary neurodegenerative disorder marked by severe neurodegeneration in the striatum and cortex. Brain-derived neurotrophic factor (BDNF) is a member of the neurotrophin family of growth factors. It plays a crucial role in maintaining the survival and proper function of striatal neurons. Depletion of BDNF has been linked to impairment and death of striatal neurons, leading to the manifestation of motor, cognitive, and behavioral dysfunctions characteristic of Huntington's disease. This review highlights the current update on the neurobiology of BDNF in the pathogenesis of Huntington's disease. The molecular evidence and the affected signaling pathways are also discussed. In addition, the impact of experimental manipulation of BDNF levels and its pharmaceutical potential for Huntington's disease treatment are explicitly reviewed.
    MeSH terms: Animals; Humans
  8. Nasrullah AA, Zander E, Dankert F, Petrov A, Surkau J, Baráth E, et al.
    Chem Sci, 2025 Mar 13.
    PMID: 40123690 DOI: 10.1039/d4sc07636b
    The 1,3-phosphaazaallene DippTerP = C=NtBu (DippTer = 2,6-(2,6-iPr2C6H3)2-C6H3) is thermally labile towards iso-butene elimination and formation of the corresponding cyanophosphine DippTerP(H)CN (1). In previous work we have shown facile deprotonation of 1 with K[N(SiMe3)2 and formation of cyanophosphide [(DippTerPCN)K]. We now present the alkali metal tethered cyanophosphides [(DippTerPCN)M(crown)] (M = Na, K; crown = 15-c-5, 18-c-6) and their structural diversity in the solid state depending on the metal (M) and the crown ether. Facile oxidation of [DippTerPCN][M(crown)] with O2 yields the formal cyanide adducts of dioxophosphoranes [DippTerPO2(CN)]-. Surprisingly, [DippTerPO2(CN)]- is obtained as a mixture of the cyanide and isocyanide isomers, indicating a coordination isomerism. This phenomenon is corroborated by experimental and theoretical studies revealing the cyanide isomer to be thermodynamically more stable. The oxidation with elemental sulphur gave the corresponding dithiophosphorane cyanide adduct [DippTerPS2(CN)]-, in which no isomerism was observed. This points to a crucial role of triplet oxygen in the isomerisation process. Monooxidation occurs when [DippTerPO2(CN)]- salts were treated with N2O, giving formal anionic phoshinidene monoxide adducts.
  9. Jang JH, Tomuleasa C, Oliynyk H, Lanamtieng T, Park J, Kim Y, et al.
    EJHaem, 2025 Apr;6(2):e70020.
    PMID: 40123793 DOI: 10.1002/jha2.70020
    INTRODUCTION: SB12 demonstrated equivalence to reference eculizumab (ECU) in complement inhibitor-naïve patients with paroxysmal nocturnal hemoglobinuria (PNH) in the previous randomized, double-blind, multi-national, crossover, Phase III study.

    METHODS: The scope of this post-hoc study was subgroup analysis by race to compare the efficacy and safety of SB12 and ECU in PNH patients in the Asian and Non-Asian subgroups of the Phase III study.

    RESULTS: Results including lactate dehydrogenase (LDH), number of units of packed red blood cells and safety as primary and secondary endpoints demonstrated comparable efficacy and safety of SB12 and ECU in Asian and Non-Asian PNH patients, in line with the study results in the overall population. In addition, transfusion avoidance (68.1% for SB12 vs. 72.9% for ECU, p-value of 0.4492) and hemoglobin stabilization (SB12-ECU: 6.3%, 95% confidence interval [CI] [-21.5, 34.1] and SB12-ECU: 2.5%, 95% CI [-24.8, 29.8] using stringent criteria) as post-hoc endpoints were not substantially different between SB12 and ECU treatment groups in the overall population as well as in Asians and Non-Asians.

    CONCLUSION: In conclusion, this subgroup analysis by race (Asians and Non-Asians) supports comparable efficacy and safety between SB12 and reference eculizumab in global PNH patients including no difference in transfusion avoidance effect.

  10. Sari KCDP, Setiati ANRN, Kusumawardani LA, Zaman Huri H, Elnaem MH
    Digit Health, 2025;11:20552076251326018.
    PMID: 40123884 DOI: 10.1177/20552076251326018
    OBJECTIVE: Like many low- and middle-income countries, Indonesia lacks specific standards for telepharmacy practices, which requires adherence to traditional pharmacy guidelines to evaluate the services. This study aims to explore the disparities between the implementation of telepharmacy services in community pharmacies and the established Indonesian pharmaceutical service standards.

    METHODS: This qualitative study was conducted in seven community pharmacies in Depok, an urban area near Jakarta. Data were collected in 2023 using triangulation methods: semi-structured interviews, direct observations, and simulated patient interactions. Verbatim transcriptions and thematic analysis were performed using NVivo®12 to analyse the findings.

    RESULTS: Four identified themes were the structures, daily practices, challenges, and enablers of telepharmacy services. The scope of telepharmacy services consisted of dispensing medicines, patient education, and providing drug information through mobile applications by pharmacy electronic system organisers (PSEF) or social media platforms. Challenges in daily practice revealed gaps in traditional pharmaceutical service guidelines, including systems interoperability, effective communication among pharmacists-physician-patients, and the need for standards specific to digital pharmaceutical services. Common problems in telepharmacy were uncertain medicine availability, unclear e-prescription validity, limited pharmacist-physician collaboration, and impeded patient education, particularly in services provided through PSEF applications. On the other hand, the availability of digital technologies was seen as beneficial, highlighting the necessity for predefined standardised facilities to ensure the quality of telepharmacy services.

    CONCLUSION: While traditional pharmaceutical service standards can be applied to evaluate telepharmacy practices, specific challenges must be explicitly addressed within a dedicated telepharmacy standard to ensure patient safety and service quality.

  11. Nuar NNA, Jamil SNAM, Choong TSY, Mahadzir SI, Azmi IDM, Basri MSM, et al.
    ACS Omega, 2025 Mar 18;10(10):10205-10219.
    PMID: 40124044 DOI: 10.1021/acsomega.4c09460
    The presence of antibiotic residues in aqueous systems, particularly doxycycline (DOX), is harmful to the environment and public health. In this study, dextran-coated calcium peroxide nanoparticles (DEX@nCPs(DEX)), Fe(II), and oxalic acid (OA) were combined to improve the heterogeneous Fenton-like degradation of DOX. X-ray photoelectron spectroscopy (XPS) demonstrated the successful synthesis of DEX@nCPs(DEX), showing the presence of Ca, O, and C functional groups associated with dextran. Using response surface methodology with a central composite design (RSM-CCD), the optimal conditions (DEX@nCPs(DEX) dosage: 2 g/L, pH: 5, contact time: 420 min) achieved 90% DOX removal, which was 20% higher than using DEX@nCPs(DEX)/Fe(II) alone. The degradation process followed first-order kinetics with a rate constant of k 1 = 0.0047 min-1. Model validation showed high predictive accuracy (R 2 = 0.996; adjusted R 2 = 0.987). Scavenger and photoluminescence analyses revealed hydroxyl radicals (•OH) to be the primary reactive species, accounting for over 80% of the degradation activity. The DEX@nCPs(DEX)/Fe(II)/OA system offers a promising approach for mitigating pharmaceutical pollutants in water, contributing to more sustainable environmental management practices.
  12. Srinivasu PN, Kumari GLA, Narahari SC, Ahmed S, Alhumam A
    Sci Rep, 2025 Mar 21;15(1):9828.
    PMID: 40119100 DOI: 10.1038/s41598-025-93505-4
    Accurately identifying bone fractures from the X-ray image is essential to prompt timely and appropriate medical treatment. This research explores the impact of hyperparameters and data augmentation techniques on the performance of the You Only Look Once (YOLO) V10 architecture for bone fracture detection. While YOLO architectures have been widely employed in object detection tasks, recognizing bone fractures, which can appear as subtle and complicated patterns in X-ray images, requires rigorous model tuning. Image augmentation was done using the image unsharp masking approach and contrast-limited adaptive histogram equalization before training the model. The augmented images assist in feature identification and contribute to overall performance of the model. The current study has performed extensive experiments to analyze the influence of hyperparameters like the number of epochs and the learning rate, along with the analysis of the data augmentation on the input data. The experimental outcome has proven that particular hyperparameter combinations, when paired with targeted augmentation strategies, improve the accuracy and precision of fracture detection. It is observed that the proposed model yielded an accuracy of 0.964 on evaluation over the augmented data. The statistical analysis of the classification precision across the augmented and raw images is observed as 0.98 and 0.95, respectively. In comparison with other deep learning models, the empirical evaluation of the YOLO V10 model clearly demonstrates its superior performance over conventional approaches for bone fracture detection.
    MeSH terms: Algorithms; Humans; Image Processing, Computer-Assisted/methods; Radiographic Image Interpretation, Computer-Assisted/methods
  13. Kim S, Matsushita Y, Katagiri T, Maseda H
    Sci Rep, 2025 Mar 21;15(1):9764.
    PMID: 40119107 DOI: 10.1038/s41598-025-94071-5
    Single-stranded oligodeoxyribonucleotide (ssODN) gene editing has emerged as a promising therapeutic strategy. However, further improvements in efficiency are desired for practical application. The effects of strand length and locked nucleic acid (LNA) modification on ssODN genome editing were investigated by introducing an assay cassette into the genome of HEK293T cells and measuring precise base deletions of eight bases. The introduction of LNAs into ssODNs, five pairs of LNAs at 25-35 nt from the centre and one pair at 20-25 nt, showed approximately 18-fold higher efficiency than unmodified ssODNs of the same length in the study using 70 nt ssODNs. In addition, genome editing efficiency was further improved when LNAs were introduced at the same positions as the 70 nt ssODN, which showed the highest efficiency for the 90 nt ssODN. However, in some cases, the same number of LNA modifications could conversely reduce the efficiency, and the modification positions in the ssODN method were successfully optimised in the present study. Furthermore, the oligo DNA was shown to be effective not only for deletions but also for base substitutions, with an editing efficiency of 0.63% per cell.
    MeSH terms: DNA, Single-Stranded/genetics; Humans; Genome, Human; HEK293 Cells; CRISPR-Cas Systems
  14. Gauhar V, Traxer O, Yuen SKK, Castellani D, Zeng G, Fong KY, et al.
    World J Urol, 2025 Mar 21;43(1):184.
    PMID: 40119185 DOI: 10.1007/s00345-025-05570-z
    BACKGROUND: Flexible and navigable suction ureteral access sheath (FANS) has been proven to improve stone-free rate (SFR) and many studies report high intraoperative 100% stone-free status (IO-SFS) and postoperative 100% stone-free status (PO-SFS). The reliability of IO-SFS vis-à-vis image-proven PO-SFS needs to be evaluated as it has implications on perioperative management.

    METHODS: Our prospective, multicenter study enrolled 704 adult patients from 21 centers who underwent FURS with FANS between August 2023 and October 2024. IO-SFS was categorized into three groups: (1) 100% SFR (no dust or fragments), (2) only dust remaining, and (3) both dust and fragments remaining. PO-SFS was assessed via 2 mm non-contrast computed tomography (NCCT) at 30 days and categorized into four grades: Grade A (100% stone-free), Grade B (single ≤ 2 mm residual fragment), Grade C (single 2.1-4 mm), and Grade D (multiple or any > 4 mm). Primary outcome was correlation between intraoperative and postoperative 100% SFS. Secondary outcomes included perioperative complications, reintervention rates, and predictors of residual fragments and perioperative complications.

    RESULTS: IO-SFS reported 100% SFR in 395 cases. Postoperative NCCT confirmed a significantly higher SFS (Grade A + B) in this group (99%) compared to 95.8% in the dust only group and 61.1% in the dust and fragments group (p 

    MeSH terms: Adult; Aged; Equipment Design; Female; Humans; Male; Middle Aged; Prospective Studies; Suction/methods; Tomography, X-Ray Computed*; Ureteroscopes
  15. Dehnavieh R, Inayatullah S, Yousefi F, Nadali M
    BMC Prim Care, 2025 Mar 21;26(1):75.
    PMID: 40119271 DOI: 10.1186/s12875-025-02773-6
    OBJECTIVE: The rapid adoption of Artificial Intelligence (AI) in health service delivery underscores the need for awareness, preparedness, and strategic utilization of AI's potential to optimize Primary Health Care (PHC) systems. This study aims to equip Iran's PHC system for AI integration by envisioning potential futures while addressing past challenges and recognizing current trends.

    METHOD: This study developed a conceptual framework based on the "Future Triangle" (FT) and the "Health Systems Governance" (HSG) models. This framework delineates the characteristics associated with the 'pulls on the future' for desired and intelligent PHC, as identified by a panel of experts. Additionally, the 'weights of the past'-referring to the challenges faced by Iran's PHC system in utilizing AI-, and the 'push of the present'-which captures the impacts of AI implementation in global primary care settings-were extracted through a review of relevant literature. The integration and analysis of the collected evidence facilitated the formulation of a range of potential future scenarios, including both optimistic and pessimistic scenarios.

    FINDINGS: The interaction between the three elements of the FT will shape the future states of Iran's PHC, whether optimistic or pessimistic. Building an optimistic scenario for an AI-driven PHC system necessitates addressing past challenges, including deficiencies in the referral and family doctor systems, the absence of evidence-based decision-making, neglect of essential community health needs, fragmented service delivery, high provider workload, and inadequate follow-up on the health status of service recipients. Consideration must also be given to the current impacts of AI in primary care, including comprehensive, coordinated, and need-based service delivery with systematic and integrated monitoring, quality improvement, early disease prevention, precise diagnosis, and effective treatment. Furthermore, fostering a shared vision among stakeholders by defining and advocating for a future system characterized by foresight, resilience, agility, adaptability, and collaboration is essential.

    CONCLUSION: Envisioning potential future states requires a balanced consideration of the influence of past, present, and future, recognizing the dual potential of AI to drive either positive or negative outcomes. Achieving the optimistic future or the "utopia of intelligent PHC" and avoiding the pessimistic future or the "dystopia of intelligent PHC" requires coherent planning, attention to the tripartite considerations of the future, past, and present, and a clear understanding of the roles, expectations, and needs of stakeholders.

    MeSH terms: Delivery of Health Care/organization & administration; Delivery of Health Care/trends; Forecasting; Humans; Iran
  16. Yi Q, Yang C, Qi Y, Feng X, Tan J, Song X, et al.
    BMC Public Health, 2025 Mar 21;25(1):1081.
    PMID: 40119281 DOI: 10.1186/s12889-025-22321-2
    BACKGROUND: The positive correlation between physical activity and cognitive function has been increasingly documented, while the underlying mechanisms remain unclear.

    METHODS: This study aimed to investigate the association among physical activity (PA), functional fitness, and cognitive function in community-dwelling older adults, and to examine whether functional fitness mediates this association. This study utilized a cross-sectional design, encompassing 224 participants aged 65 or above, with 41.07% male and 58.93% female. The data were collected in 2023 using the Mini-Mental State Examination, Physical Activity Scale for the Elderly, and Senior Fitness Test. Pearson correlation analysis was conducted to assess associations among the variables, and mediation analysis was performed using the PROCESS macro for SPSS.

    RESULTS: The results indicated that PA had a moderate positive correlation with cognitive function (r = 0.437, p 

    MeSH terms: Aged; Aged, 80 and over; Cross-Sectional Studies; Female; Humans; Male; Independent Living*
  17. Marasco G, Hod K, Colecchia L, Cremon C, Barbaro MR, Cacciari G, et al.
    PMID: 40119532 DOI: 10.1002/ueg2.70005
    BACKGROUND: The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic has highlighted the potential exacerbation of gastrointestinal symptoms in patients with disorders of gut-brain interaction (DGBIs). However, the distinct symptom trajectories and psychological burden in patients with post-COVID-19 DGBIs compared with patients with pre-existing irritable bowel syndrome (IBS)/functional dyspepsia (FD) and non-DGBI controls remain poorly understood.

    OBJECTIVES: To examine the long-term gastrointestinal symptom progression and psychological comorbidities in patients with post-COVID-19 DGBI, patients with pre-existing IBS/FD and non-DGBI controls.

    METHODS: This post hoc analysis of a prospective multicenter cohort study reviewed patient charts for demographic data and medical history. Participants completed the Gastrointestinal Symptom Rating Scale at four time points: baseline, 1, 6, and 12 months, and the Hospital Anxiety and Depression Scale at 6 and 12 months. The cohort was divided into three groups: (1) post-COVID-19 DGBIs (2) non-DGBI, and (3) pre-existing IBS/FD, with the post-COVID-19 DGBIs group compared to the latter two control groups.

    RESULTS: Among 599 eligible patients, 27 (4.5%) were identified as post-COVID-19 DGBI. This group experienced worsening abdominal pain, hunger pain, heartburn, and acid regurgitation, unlike symptom improvement or stability in non-DGBI controls (p 

  18. Edwards G, Spooner M, Arnett R, Kelly H, Carr JCA, Illing J
    Med Educ, 2025 Mar 22.
    PMID: 40119725 DOI: 10.1111/medu.15660
    BACKGROUND: Medical education is becoming more internationalised. Increasing numbers of students engage in Transnational Medical Education Programmes, pursuing their Primary Medical Qualification outside of their home country; with plans to transition back to their home country - or a third country - after graduation. Medical education is however, inextricably embedded within medical contexts, and a potential mismatch exists between graduates' place of training and place of practice in terms of the knowledge, skills and values required to practice safely in a different context.

    AIM: What types of Transnational Medical Education programmes can be identified from the international literature? What are the strengths and weaknesses of these programmes and do they prepare students for international practice?

    METHODS: This review was based on the PRISMA-Guidelines for systematic reviews. We searched five electronic databases: MEDLINE, EMBASE, CINAHL, WEB of Science and ERIC. No date or language restrictions were placed on the inclusion criteria. Data extraction was completed by two independent reviewers. Quality appraisal was carried out using the Mixed Methods Appraisal Tool (MMAT), and a narrative synthesis of the included papers was conducted.

    RESULTS: Our literature search identified 12 studies. From the included studies we were able to identify three types of Transnational Medical Education Programme, and a number of strengths and weaknesses of these programmes. Strengths were: increasing medical workforce capacity, enhancing the quality of education locally, promoting intercultural competence and increasing exposure to new ways of working (enhanced communication skills, shared decision-making, experience of multidisciplinary teamwork and patient-centeredness). Weaknesses were: potential curriculum misalignment within the context of clinical practice, language barriers and encountering differences in hierarchical systems, shared decision making and patient-centredness all of which impact preparation for practice.

    CONCLUSION: Transnational medical education programmes address shortages within the medical workforce by creating additional training opportunities. They can also drive up the quality of medical education locally. There is, however, a potential mismatch between the curriculum and the place of practice which can impact upon graduates' preparation for professional practice.

  19. Nik Najhannor NAQ, Mohd Fauzi N, Talip BA, Zaaba MAM, Mhd Ali A, Mohd Tahir NA, et al.
    Biochem Mol Biol Educ, 2025 Mar 22.
    PMID: 40119740 DOI: 10.1002/bmb.21893
    In modern education, mobile applications offer flexibility for learning anytime and anywhere, yet biochemistry lacks mobile-based virtual laboratories. This study aimed to develop a 2D Mobile-Based Virtual Laboratory Simulation (2D-MViLS) for blood glucose level measurement and evaluate its impact on pharmacy students' learning experience. In stage 1, storyboards were developed and expanded to show the overall flow and simulation in interactive 2D form using Unity for Android devices. In stage 2, usability testing was carried out. In Stage 3, reflective essays from first-year pharmacy students were analyzed using both qualitative and quantitative methods. Three key themes were identified: students' feelings, their understanding of the learning outcomes, and their reflections on the need to enhance their current knowledge and skills. Thematic analysis revealed a positive shift in students' feelings after using the 2D-MViLS. Quantitatively, 44% of students demonstrated a highly positive change, moving from pessimistic to optimistic feelings, 17% showed moderate positive change, and 51% expressed optimism before and after the simulation. Quiz marks were consistently high across all groups, with minimal variation regardless of the number of learning outcomes mentioned. Additionally, 73.9% of students identified areas for knowledge and/or skill enhancement, highlighting their engagement with the simulation. In conclusion, students' reflections reveal the positive impact of 2D-MViLS on their learning experience, indicating that implementing mobile-based virtual laboratories could significantly enhance the learning experience in biochemistry education and potentially lead to improved teaching methods in pharmacy and related disciplines.
  20. Zhou L, Ali I, Manickam S, Goh BH, Tao Y, Zhang J, et al.
    Compr Rev Food Sci Food Saf, 2025 Mar;24(2):e70162.
    PMID: 40119796 DOI: 10.1111/1541-4337.70162
    Consumers' growing demand for healthy and natural foods has led to a preference for products with fewer additives. However, the low emulsifying properties of natural proteins often necessitate the addition of emulsifiers in food formulations. Consequently, enhancing the emulsifying properties of proteins through various modification methods is crucial to meet modern consumer demands for natural food products. High-intensity ultrasound offers a green, efficient processing technology that significantly improves the emulsifying properties of proteins. This study explores how ultrasound treatment enhances the stability of protein-based emulsions by modifying protein structures. While ultrasonic treatment does not significantly affect the primary structure of proteins, it influences the secondary, tertiary, and quaternary structures depending on the type of protein, ultrasound parameters (type, intensity, and time), and treatment conditions. The results suggest that ultrasound treatment reduces α-helix content, decreases protein particle size, and increases β-sheet content, surface hydrophobicity, free sulfhydryl groups, and zeta potential, leading to a more stable protein-based emulsion. The reduced particle size and increased flexibility of proteins induced by ultrasound enable more rapid protein adsorption at the oil-water interface, resulting in smaller emulsion droplets. This contributes to the emulsion's improved stability during storage. Future research should focus on the large-scale application of ultrasonic treatment for protein modification to produce high-quality, natural foods that meet the evolving needs of consumers.
    MeSH terms: Ultrasonic Waves; Food Handling/methods; Particle Size; Proteins/chemistry; Ultrasonics/methods; Emulsifying Agents/chemistry; Hydrophobic and Hydrophilic Interactions
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