Browse publications by year: 2025

  1. Ramin NM, Salleh WMNHW, Salihu AS, Ab Ghani N, Agosto NJ, Sungthong B, et al.
    Nat Prod Res, 2025 Feb 01.
    PMID: 39891598 DOI: 10.1080/14786419.2025.2457117
    Piperaceae family includes numerous species used for food, spices, and medicinal purposes. In this study, the chemical composition and bioactivities (antioxidant, anticholinesterase, and anti-inflammatory activities) of the essential oil from Piper rostratum Roxb. were investigated. The essential oil was extracted via hydrodistillation and thoroughly analysed using gas chromatography-flame ionisation detection (GC-FID) and gas chromatography-mass spectrometry (GC-MS). A total of 20 chemical components were identified, accounting for 94.2% of the total oil. The major components were γ-muurolene (14.1%), δ-cadinene (13.2%), allylpyrocatechol diacetate (11.5%), chavicol (8.2%), α-humulene (7.8%), and hydroxychavicol (6.9%). The antioxidant activity was evaluated using the DPPH free radical scavenging assay, the acetylcholinesterase inhibitory activity was measured using the Ellman method, and the anti-inflammatory activity was assessed through lipoxygenase enzyme inhibition. The essential oil demonstrated moderate activity in DPPH free radical scavenging (IC50 = 98.5 µg/mL), acetylcholinesterase inhibition (IC50 = 89.2 µg/mL), and lipoxygenase inhibition (IC50 = 77.2 µg/mL).
  2. William A, Lachat C, Ahles S, Murphy KJ, Minihane AM, Weaver C, et al.
    Eur J Nutr, 2025 Feb 01;64(2):76.
    PMID: 39891676 DOI: 10.1007/s00394-024-03561-1
    PURPOSE: Inadequate reporting of nutrition data can hinder the success of nutrition health policies. CONSORT provides guidance for reporting of randomised controlled trials (RCTs) and is required by most journals today, yet reporting of nutrition interventions may benefit from a more tailored approach. A Federation of European Nutrition Societies working group was created to improve quality and completeness of reporting of nutrition trials, and our work to date features a proposal for a CONSORT extension specific to nutrition RCTs. The present manuscript describes a Delphi survey conducted to gather opinion from a wider panel of nutrition and health experts and related interest-holders on our proposal.

    METHODS: We invited 138 potentially eligible participants to take part in the Delphi survey from a representative spread of expertise and geography. We employed a Likert scale with comments for our 32-item proposal in round 1, and a dichotomous scale with comments for our 29-item proposal in round 2. Threshold for agreement was set at ≥ 80% for both rounds.

    RESULTS: Forty-seven potentially eligible participants responded to our invitation, 38 completed the first round and 36 completed the second. N = 23 (72%) items achieved ≥ 80% in round 1, and 100% of items in round 2. Three items were dropped or merged following round 1. A third Delphi round was not required to obtain consensus.

    CONCLUSIONS: This Delphi expert consensus proposes a 29-item checklist specific to the reporting of nutrition RCTs and will inform further development of guidance through forthcoming consensus meetings.

    MeSH terms: Delphi Technique*; Humans; Surveys and Questionnaires; Research Design/standards; Nutrition Policy; Consensus*; Nutritional Sciences/standards; Checklist
  3. Luo JX, Gao XT, Rong Z, Zhang LH, Sun YF, Qi ZL, et al.
    Mar Biotechnol (NY), 2025 Feb 01;27(1):41.
    PMID: 39891779 DOI: 10.1007/s10126-025-10420-5
    Mandarin fish (Siniperca chuatsi) is a voracious carnivorous species, usually consuming only live bait fish, but dietary acclimation enables it to accept artificial feed. However, the effects of dietary acclimation on intestinal performance and gene expression in mandarin fish and related mechanisms remain largely unknown. Therefore, this study investigated the effects of artificial feed on intestinal physicochemical and biochemical performance and gene expression in mandarin fish. Mandarin fish were sampled on day 10 after feeding with live dace (LD), at day 40 after subsequent feeding with dead dace plus artificial feed (DD + AF) from day 11 to day 40, and at day 90 after continuous feeding with artificial feed (AF) alone from day 41 to day 90 for transcriptome sequencing. The biochemical analysis results indicated that artificial feed significantly increased the activity of antioxidant enzymes glutathione peroxidase and superoxide dismutase in the intestine, liver, and stomach. Histological analysis demonstrated intestinal damage in mandarin fish fed with artificial feed. The GO and KEGG enrichment analyses indicated that the DEGs in AF vs. DD + AF were significantly enriched in the pentose phosphate pathway, and the DEGs in AF vs. LD were mainly significantly enriched in glycolysis/gluconeogenesis and PPAR signaling pathways. Nineteen feed acclimation-related key genes such as gene pfkfb4a and scd were identified in the intestine and found to exhibit upregulated expressions. These results revealed that artificial feed domestication enhanced the antioxidant capacity of the mandarin fish intestine and reduced hepatic lipid deposition by upregulating the related gene expression of mandarin fish and that the regulation of carbon metabolisms, including sugar, lipid, and steroid metabolisms, might be fundamental mechanisms for mandarin fish to acclimatize to dietary changes. These findings provide novel insights into the feed acclimation mechanism of mandarin fish, holding implications for promoting large-scale artificial feed aquaculture of mandarin fish and improving economic efficiency.
    MeSH terms: Animals; Animal Feed*; Diet/veterinary; Intestines*; Perciformes/genetics; Perciformes/metabolism; Aquaculture; Gene Expression Profiling; Transcriptome*; Domestication
  4. Shi Y, Deng C, Lu X, Wang Y, Pan Y, Su D, et al.
    Cell Rep, 2025 Jan 31;44(2):115258.
    PMID: 39891905 DOI: 10.1016/j.celrep.2025.115258
    Cuticles protect plants from water loss and pathogen attack. We address here the functional significance of SlGRAS9, SlZHD17, and SlMBP3 in regulating cutin formation in tomato fruit. The study unveils the role of the multipartite "SlGRAS9-SlZHD17-SlMBP3-SlMIXTA-like" transcription factor module in cutin biosynthesis. Plants deficient in SlGRAS9, SlZHD17, or SlMBP3 exhibit thickened cuticles and a higher accumulation of cutin monomers, conferring extended fruit shelf life and higher tolerance to postharvest fungal infection. SlGRAS9 regulation of cutin is mediated by SlZHD17, a negative regulator of SlCYP86A69. SlZHD17 acts synergistically with SlMBP3 to repress SlCYP86A69, and its interaction with SlMIXTA-like prevents the binding to the SlCYP86A69 promoter, thereby releasing the repression of cutin biosynthesis. SlZHD17 and SlMBP3 synergistically repress cutin biosynthesis, while SlMIXTA-like and SlCD2 act antagonistically to SlZHD17 and SlMBP3 on this metabolic pathway. The study defines targets for breeding strategies aimed at improving cuticle-associated traits in tomato and potentially other crops.
  5. Zou S, Zhang Z, Lee YY, Ai H, Zhang Y, Wang Y
    Food Chem, 2025 Jan 31;474:143151.
    PMID: 39891972 DOI: 10.1016/j.foodchem.2025.143151
    Acylglycerols are widely used in the food industry due to their antimicrobial, emulsifying and nutritional properties. This study investigated the key reaction parameters, mass transfer mechanisms, and kinetic features of lipase-catalyzed esterification in a solvent-free system. Taguchi method was further employed to explore the relationship between "reaction parameter - yield composition". The results revealed that the maximum selectivity were achieved fror MAG (57.72 %), DAG (82.67 %) and TAG (79.29 %) under different enzymatic conditions. Fatty acid-to-glycerol molar ratio had the greatest impact on DAG selectivity, contributing 38.08 % of total impact level. Mass transfer analysis showed that external mass transfer limitation could be effectively overcome at stirring speeds above 600 rpm. Kinetic analysis confirmed that the reaction followed the Ping-Pong BiBi mechanism with alcohol and acid inhibition (SSE = 0.000643). This work provided a theoretical basis for developing more efficient and selective catalytic processes, aiding in quality control, reactor design, and industrial scale-up.
  6. Meteku BT, De Silva K, Green SE, Turner T
    J Clin Epidemiol, 2025 Jan 30;180:111707.
    PMID: 39892522 DOI: 10.1016/j.jclinepi.2025.111707
    OBJECTIVES: Evidence-based clinical guidelines have the potential to improve health care and health outcomes. Living guidelines methods provide an approach to ensuring guidelines are always up-to-date, maximizing this potential. However, to date, most work on living guidelines has been conducted in high income countries. The objective of this study is to explore the barriers and facilitators to the development, adaptation, and use of living guidelines among evidence-based guideline developers in low- and middle-income countries (LMICs).

    STUDY DESIGN AND SETTING: We used a descriptive qualitative study design. We employed purposive and snowball sampling techniques to recruit guideline developers from LMICs and World Health Organization offices to participate in online, semistructured interviews. Data were analysed using a thematic approach with NVivo 20 software. Ethics approval was granted by Monash University.

    RESULTS: We interviewed 18 participants from LMICs (Colombia, India, Iran, Indonesia, Argentina, and Malaysia) and WHO offices (including headquarters, regional offices, and country offices). Two main themes emerged, along with six associated subthemes. The main themes were as follows: (1) People in LMICs want living guidelines and (2) Resource limitations and their implications for living guidelines in LMICs.

    CONCLUSION: Our research identified that guideline developers in LMICs have a strong desire to both develop and use living guidelines, but are currently limited by important barriers. Initiatives to support development, adaptation, and use of living guidelines in LMICs may help overcome barriers and meet the need for living guidelines in LMICs. It is also essential to design strategies that overcome identified barriers to developing, adapting, and implementing living guidelines, such as a lack of resources, delays in updates, and limited accessibility.

    PLAIN LANGUAGE SUMMARY: This study looked at how living (continuously updated) approaches can be used to develop, adapt, and use clinical guidelines in settings with limited resources, and explored the pros and cons of each. The findings revealed a strong need to develop and use living guidelines in low-resource settings despite challenges, such as resource scarcity, delays in updating, and limited access to these guidelines. Overall, the findings revealed that living guidelines were worthwhile in LMICs despite all of the related challenges.

  7. Thuya WL, Cao Y, Ho PC, Wong AL, Wang L, Zhou J, et al.
    PMID: 39893129 DOI: 10.1016/j.cytogfr.2025.01.003
    The IL-6/JAK/STAT3 signaling pathway is a key regulator of tumor progression, immune evasion, and therapy resistance in various cancers. Frequently dysregulated in malignancies, this pathway drives cancer cell growth, survival, angiogenesis, and metastasis by altering the tumor microenvironment (TME). IL-6 activates JAK kinases and STAT3 through its receptor complex, leading to the transcription of oncogenic genes and fostering an immunosuppressive TME. This environment recruits tumor-associated macrophages (TAMs), cancer-associated fibroblasts (CAFs), and regulatory T cells (Tregs), collectively supporting immune evasion and tumor growth. IL-6/JAK/STAT3 axis also contributes to metabolic reprogramming, such as enhanced glycolysis and glutathione metabolism, helping cancer cells adapt to environmental stresses. Therapeutic targeting of this pathway has gained significant interest. Strategies include monoclonal antibodies against IL-6 or its receptor (e.g., Tocilizumab, Siltuximab), JAK inhibitors (e.g., Ruxolitinib), and STAT3-specific inhibitors (e.g., Napabucasin), which have exhibited promise in preclinical and initial clinical studies. These inhibitors can suppress tumor growth, reverse immune suppression, and enhance the efficacy of immunotherapies like immune checkpoint inhibitors. Combination therapies that integrate IL-6 pathway inhibitors with conventional treatments are particularly promising, addressing resistance mechanisms and improving patient outcomes. Advances in biomarker-driven patient selection, RNA-based therapies, and isoform-specific inhibitors pave the way for more precise interventions. This review delves into the diverse roles of IL-6/JAK/STAT3 signaling in cancer progression, therapeutic strategies targeting this pathway, and the potential for integrating these approaches into personalized medicine to enhance treatment outcomes.
  8. Kow CS, Ramachandram DS, Hasan SS, Thiruchelvam K
    J Mycol Med, 2025 Jan 16;35(1):101534.
    PMID: 39892065 DOI: 10.1016/j.mycmed.2025.101534
    INTRODUCTION: Vulvovaginal candidiasis (VVC) is a prevalent fungal infection affecting millions of women globally, primarily caused by Candida species, most notably Candida albicans. Ibrexafungerp emerges as a promising candidate in the treatment arsenal against VVC, presenting a novel approach to combating this prevalent fungal infection.

    METHODS: A systematic literature search was conducted across major databases, including PubMed, EMBASE, and the Cochrane Library, to identify relevant randomized controlled trials (RCTs) evaluating the efficacy and safety of ibrexafungerp in the treatment of VVC. Following rigorous methodology, data extraction, risk of bias assessment using Cochrane's RoB 2 tool, and meta-analysis were conducted.

    RESULTS: Four RCTs were included in the analyses. The ibrexafungerp regimen utilized across the studies were 300 mg administered twice daily for one day. Meta-analysis revealed that ibrexafungerp was associated with significantly higher clinical cure rates compared to placebo in patients with VVC (pooled odds ratio (OR) 2.32; 95 % confidence interval (CI) 1.80 to 2.98). Complete symptom resolution was achieved in a greater proportion of participants receiving ibrexafungerp (pooled OR 2.76; 95 % CI 1.62 to 4.71). Analysis of treatment-emergent adverse events revealed a significant higher incidence of at least one treatment-emergent adverse event with ibrexafungerp compared to placebo (pooled OR 2.83; 95 % CI 2.06 to 3.88).

    CONCLUSION: This study provides robust support for the efficacy of ibrexafungerp in the treatment of VVC. While the safety profile of ibrexafungerp appears favorable with mostly mild adverse events reported, decision-making in the clinical context should be guided by individual patient factors.

  9. Hassan BAR, Mohammed AH
    J Infect Chemother, 2025 Jan 30;31(4):102638.
    PMID: 39892512 DOI: 10.1016/j.jiac.2025.102638
    INTRODUCTION: Bacterial resistance poses a significant threat to cancer patients due to their compromised immune systems and frequent use of antibiotics. Understanding oncologists' knowledge of this issue is essential for improving patient care. This study aimed to develop and validate a questionnaire assessing oncologists' knowledge of the relationship between bacterial resistance and cancer.

    METHODS: A cross-sectional study involving 421 participants was conducted in Iraq from November 2023 to March 2024 across various cancer centers and oncology hospitals. A 41-item questionnaire covering eight domains-Risk Factors, Infections and Antibiotic Use, Mechanisms of Resistance, Impact on Drug Efficacy and Toxicity, Genetic Factors, Microbial Adaptation, Impact of Gut Microbiota, and Antimicrobial Stewardship-was developed. Content validity was evaluated by five experts. Exploratory Factor Analysis (EFA) and Confirmatory Factor Analysis (CFA) were used to assess construct validity, while Item Response Theory (IRT) evaluated item performance. Reliability was measured using Cronbach's Alpha and Composite Reliability (CR).

    KEY FINDINGS: The questionnaire showed strong content validity, with most items achieving a Content Validity Index (CVI) of ≥0.8. EFA identified eight factors, confirmed by CFA with acceptable model fit (CFI = 0.96, TLI = 0.95, RMSEA = 0.05). IRT indicated strong item discrimination and acceptable difficulty. Cronbach's Alpha was 0.88, with CR at 0.89.

    CONCLUSION: This validated 41-item questionnaire provides a reliable tool for assessing oncologists' knowledge of bacterial resistance among cancer patients. It helps identify knowledge gaps and guide interventions to improve oncology practice outcomes.

  10. Brodie JF, Bello C, Emer C, Galetti M, Luskin MS, Osuri A, et al.
    Conserv Biol, 2025 Feb;39(1):e14414.
    PMID: 39466005 DOI: 10.1111/cobi.14414
    The urgent need to mitigate and adapt to climate change necessitates a comprehensive understanding of carbon cycling dynamics. Traditionally, global carbon cycle models have focused on vegetation, but recent research suggests that animals can play a significant role in carbon dynamics under some circumstances, potentially enhancing the effectiveness of nature-based solutions to mitigate climate change. However, links between animals, plants, and carbon remain unclear. We explored the complex interactions between defaunation and ecosystem carbon in Earth's most biodiverse and carbon-rich biome, tropical rainforests. Defaunation can change patterns of seed dispersal, granivory, and herbivory in ways that alter tree species composition and, therefore, forest carbon above- and belowground. Most studies we reviewed show that defaunation reduces carbon storage 0-26% in the Neo- and Afrotropics, primarily via population declines in large-seeded, animal-dispersed trees. However, Asian forests are not predicted to experience changes because their high-carbon trees are wind dispersed. Extrapolating these local effects to entire ecosystems implies losses of ∼1.6 Pg CO2 equivalent across the Brazilian Atlantic Forest and 4-9.2 Pg across the Amazon over 100 years and of ∼14.7-26.3 Pg across the Congo basin over 250 years. In addition to being hard to quantify with precision, the effects of defaunation on ecosystem carbon are highly context dependent; outcomes varied based on the balance between antagonist and mutualist species interactions, abiotic conditions, human pressure, and numerous other factors. A combination of experiments, large-scale comparative studies, and mechanistic models could help disentangle the effects of defaunation from other anthropogenic forces in the face of the incredible complexity of tropical forest systems. Overall, our synthesis emphasizes the importance of-and inconsistent results when-integrating animal dynamics into carbon cycle models, which is crucial for developing climate change mitigation strategies and effective policies.
    MeSH terms: Animals; Carbon/metabolism; Conservation of Natural Resources*; Trees/physiology; Tropical Climate*; Climate Change*; Carbon Cycle*; Seed Dispersal; Herbivory; Forests; Rainforest*
  11. Midot F, Goh KM, Liew KJ, Lau SYL, Espenberg M, Mander Ü, et al.
    Appl Environ Microbiol, 2025 Jan 31;91(1):e0198624.
    PMID: 39714193 DOI: 10.1128/aem.01986-24
    Tropical peatlands significantly influence local and global carbon and nitrogen cycles, yet they face growing pressure from anthropogenic activities. Land use changes, such as peatland forests conversion to oil palm plantations, affect the soil microbiome and greenhouse gas (GHG) emissions. However, the temporal dynamics of microbial community changes and their role as GHG indicators are not well understood. This study examines the dynamics of peat chemistry, soil microbial communities, and GHG emissions from 2016 to 2020 in a logged-over secondary peat swamp forest in Sarawak, Malaysia, which transitioned to an oil palm plantation. This study focuses on changes in genetic composition governing plant litter degradation, methane (CH4), and nitrous oxide (N2O) fluxes. Soil CO2 emission increased (doubling from approximately 200 mg C m-2 h-1), while CH4 emissions decreased (from 200 µg C m-2 h-1 to slightly negative) following land use changes. The N2O emissions in the oil palm plantation reached approximately 1,510 µg N m-2 h-1, significantly higher than previous land uses. The CH4 fluxes were driven by groundwater table, humification levels, and C:N ratio, with Methanomicrobia populations dominating methanogenesis and Methylocystis as the main CH4 oxidizer. The N2O fluxes correlated with groundwater table, total nitrogen, and C:N ratio with dominant nirK-type denitrifiers (13-fold nir to nosZ) and a minor role by nitrification (a threefold increase in amoA) in the plantation. Proteobacteria and Acidobacteria encoding incomplete denitrification genes potentially impact N2O emissions. These findings highlighted complex interactions between microbial communities and environmental factors influencing GHG fluxes in altered tropical peatland ecosystems.IMPORTANCETropical peatlands are carbon-rich environments that release significant amounts of greenhouse gases when drained or disturbed. This study assesses the impact of land use change on a secondary tropical peat swamp forest site converted into an oil palm plantation. The transformation lowered groundwater levels and changed soil properties. Consequently, the oil palm plantation site released higher carbon dioxide and nitrous oxide compared to previous land uses. As microbial communities play crucial roles in carbon and nitrogen cycles, this study identified environmental factors associated with microbial diversity, including genes and specific microbial groups related to nitrous oxide and methane emissions. Understanding the factors driving microbial composition shifts and greenhouse gas emissions in tropical peatlands provides baseline information to potentially mitigate environmental consequences of land use change, leading to a broader impact on climate change mitigation efforts and proper land management practices.
    MeSH terms: Agriculture; Bacteria/classification; Bacteria/genetics; Bacteria/isolation & purification; Bacteria/metabolism; Carbon/analysis; Carbon/metabolism; Malaysia; Methane/analysis; Methane/metabolism; Nitrogen/analysis; Nitrogen/metabolism; Nitrous Oxide/analysis; Nitrous Oxide/metabolism; Soil/chemistry; Soil Microbiology*; Arecaceae; Wetlands; Carbon Cycle; Nitrogen Cycle; Microbiota*; Forests*
  12. Letchumanan G, Marlini M, Baharom N, Lawley B, Syed Mohideen FB, Jogulu SR, et al.
    J Med Microbiol, 2025 Jan;74(1).
    PMID: 39886920 DOI: 10.1099/jmm.0.001963
    Introduction. Type 2 diabetes mellitus (T2DM) is a major global health issue projected to exceed 700 million cases by 2045. In Malaysia, T2DM prevalence has risen, with notable ethnic disparities.Gap statement. The gut microbiota's role in T2DM pathogenesis is well recognized, yet its composition in Malaysia's ethnically diverse population remains underexplored.Aim. This study aimed to characterize gut microbiota composition among T2DM and ethnicity-matched adults without diabetes (nonDM) in Malaysia.Methodology. A case-control study was conducted with 45 T2DM and 45 nonDM participants matched by ethnicity from a primary care clinic in Klang Valley, Malaysia. Faecal DNA was subjected to 16S rRNA sequencing to identify microbiota diversity and composition differences and compare predicted functional capabilities. Correlations between bacterial taxa, clinical characteristics and dietary intake were analysed.Results. T2DM participants showed decreased alpha diversity (observed, P-value=0.002, r=0.69; Shannon, P-value<0.001, r=0.73) and significant differences in beta diversity (permutational multivariate ANOVA, R²=0.036, P-value=0.001). Linear discriminant analysis effect size and multiple regression analysis, adjusted for covariates age, gender, BMI and intakes of protein, fat, carbohydrate and fibre, identified the phylum Proteobacteria and genera Escherichia-Shigella to be increased, while the genera Anaerostipes and Romboutsia decreased in T2DM. These bacteria were associated with various clinical characteristics and dietary intake. However, these 'potential biomarkers' were not uniformly present across all participants, suggesting that individual bacterial taxa may not serve as universal biomarkers.Conclusion. Significant gut microbiota differences exist between T2DM and nonDM individuals in Malaysia, indicating a dysbiosis characterized by increased pro-inflammatory bacteria and reduced short-chain fatty acid-producing bacteria in T2DM. While these findings highlight the potential functional relevance of gut microbiota in T2DM pathogenesis, addressing limitations such as participant matching for confounding factors in future studies could uncover additional significant differences in microbiota composition. Furthermore, the variability in taxa prevalence across individuals suggests that targeting microbial metabolic products may offer more promising strategies to inform microbiota-targeted interventions than relying solely on specific bacterial taxa as biomarkers.
    MeSH terms: Gastrointestinal Microbiome*; Adult; Aged; Bacteria/classification; Bacteria/genetics; Bacteria/isolation & purification; Ethnic Groups; Female; Humans; Malaysia/epidemiology; Male; Middle Aged; Case-Control Studies
  13. Bushra SMR, Bakar RA, Nurul AA
    J Asian Nat Prod Res, 2025 Jan 31.
    PMID: 39887028 DOI: 10.1080/10286020.2025.2453852
    Lignosus rhinocerotis is rich in polysaccharide with diverse -bioactivities. This study developed a pre-column derivatization reversed-phase high-performance liquid chromatography (RP-HPLC) method for analyzing monosaccharides in Lignosus rhinocerotis polysaccharides (LRP). LRP underwent hydrolysis, derivatization, and separation on a Cosmosil 5C18-MS-II column at 254 nm. Baseline separation of eight standard monosaccharides was achieved within 45 min. Calibration curves, precision, and accuracy were validated. Quantitative analysis revealed LRP as a heteropolysaccharide containing mannose, ribose, rhamnose, glucose, galactose, xylose, and arabinose, with 100.28-111.02% recovery. This optimized RP-HPLC offers a simple, reproducible, and accurate tool for LRP monosaccharides analysis, facilitating in understanding its structure-function relationship.
  14. Morales-Espinoza LL, Gebara RC, Longo E, Fracácio R
    Environ Toxicol Chem, 2025 Jan 01;44(1):3-25.
    PMID: 39887287 DOI: 10.1093/etojnl/vgae008
    Ecotoxicological studies involving microplastics (MPs) conducted on a laboratory scale may not always accurately mirror real environmental conditions (types, sizes, shapes, and concentrations of MPs). This review primarily focused on examining studies that investigated the prevalence of MPs in freshwater environments and accumulation in organisms worldwide, considering important factors such as morphology, particle size, and the specific polymer types involved. This review also encompassed ecotoxicological studies related to MPs, and ecological risk analyses were carried out based on the obtained ecotoxicological data. Based on the extensive dataset collected, we determined the hazardous concentration for 5% of the tested organisms (HC5) and estimated for the first time a predicted noneffect concentration (PNEC) value for two distinct types of MPs polymers: polystyrene (PS) and polyethylene (PE), based on the species sensitive distribution (SSD) curves obtained from nonobserved concentration (NOEC) values, with 0.003 mg L-1 for PS and 0.011 mg L-1 for PE. Furthermore, another PNEC value for 7 types of MPs (PE, PS, polyvinyl chloride, PA6, polyester, polyethylene terephthalate, and ethylene acrylic acid copolymer (EEA copolymer)) was calculated (0.0027 mg L-1 or 2.61 particles L-1). Subsequently, the risk quotient (RQ) was computed utilizing data obtained from the measured environmental concentrations of 18 places. An RQ value of 0.094 was obtained, inferring that MPs have a low-risk potential globally. However, when the RQ values were examined for each country separately, they exhibited significant variability (RQ = 22.06 in Malaysia and 0.000008 in Australia). Overall, this review provides a comprehensive overview of the current knowledge on MP abundance in freshwater environments, the associated ecotoxicological research to reinforce the outcomes derived from the risk analysis, and their accumulation in biota.
    MeSH terms: Animals; Environmental Monitoring/methods; Ecosystem; Risk Assessment; Ecotoxicology*
  15. Ghadin N, Baharum SN, Ahmad Raston NH, Low CF
    J Fish Dis, 2025 Jan 31.
    PMID: 39887434 DOI: 10.1111/jfd.14093
    White tail disease in Macrobrachium rosenbergii is caused by M. rosenbergii nodavirus (MrNV) infection, resulting in up to 100% mortality in larvae and post-larvae stages, severely impacting aquaculture production. Existing genome-based detection methods for MrNV are costly and time-consuming, highlighting the need for rapid and cost-effective diagnostic tests. This study evaluated the effects of truncating selected aptamer on its binding affinity to the MrNV capsid protein. The previously isolated and identified aptamer through magnetic-capture SELEX and Next Generation Sequencing demonstrated high binding affinity to the MrNV capsid protein. Truncation at the primer overhang was found to improve binding affinity, reducing the dissociation constant from 347 nM to 30.1 nM. The calculated limit of detection for the truncated aptamer decreased from 5.64 nM to 1.7 nM, while the limit of quantification decreased from 17.1 nM to 5.16 nM. These reductions indicate that the truncated aptamer has higher sensitivity compared to the full-length aptamer. In tests with MrNV-infected M. rosenbergii samples, both the enzyme-linked aptamer assay and the gold nanoparticle aptasensor assay showed consistent results when 0.5 μg of total protein lysate was used. This indicates that the prawn protein concentration interferes with the detection of the viral protein. These findings suggest the potential application of the truncated aptamer as a sensor in the development of a practical aptamer-based diagnostic kit. For instance, an aptamer-based lateral flow assay test kit could provide a user-friendly, cost-effective solution that eliminates the need for sophisticated instrumentation for diagnosis or data interpretation, making it ideal for detecting MrNV infection in M. rosenbergii aquaculture.
  16. Tan KA, Ryan MM, Kennedy RA, Carroll K, de Valle K, Kollias CM, et al.
    J Peripher Nerv Syst, 2025 Mar;30(1):e70002.
    PMID: 39887493 DOI: 10.1111/jns.70002
    BACKGROUND AND AIMS: Despite the known association of hip dysplasia and Charcot Marie Tooth disease (CMT), evidence is limited regarding its exact prevalence. Available studies pre-date genetic confirmation of CMT subtypes and current hip reconstruction surgical options. This study examined the prevalence of hip dysplasia in CMT in a tertiary neuromuscular center.

    METHODS: This was a retrospective study of children with CMT who had at least one pelvic radiograph between 2000 and 2020. Reimer's migration percentage, acetabular index and lateral center edge angle were used to identify hip dysplasia.

    RESULTS: A total of 178 children were included with a median age of 6.4 (IQR 3.4-11.3) years at CMT diagnosis. First pelvic radiographs were performed at a median age of 8.0 (IQR 4.6-12.2) years and 64 (35.8%) had hip dysplasia, of which 20 normalized over time. Repeat radiographs were done in 96/178 children (53.9%), and six children with originally normal radiographs developed later radiographic hip dysplasia. At the time of last follow up, 50/178 children (28.1%) had hip dysplasia and 17/178 children (9.6%) required surgical intervention. The frequency of hip dysplasia in specific CMT subtypes was: 28/100 in CMT1A, 5/7 in Dejerine-Sottas disease, 3/10 in CMT2A, and 4/4 in TRPV4-related CMT.

    INTERPRETATION: The prevalence of hip dysplasia in children with CMT in this cohort was estimated to be between 9.6% and 28.1%. Serial imaging is important to monitor outcomes into adulthood. Specific CMT subtypes were more likely to be associated with hip dysplasia.

    MeSH terms: Adolescent; Child; Child, Preschool; Female; Hip Dislocation/etiology; Hip Dislocation/epidemiology; Humans; Male; Retrospective Studies; Cohort Studies; Prevalence
  17. Khan KS, Fawzy M, Chien P, Geary M, Bueno-Cavanillas A, Nunez-Nunez M, et al.
    Int J Gynaecol Obstet, 2025 Jan 31.
    PMID: 39887735 DOI: 10.1002/ijgo.16118
    The number of retractions of randomized clinical trials (RCTs) following post-publication allegations of misconduct is increasing. To address this issue, we aimed to establish an international multistakeholder consensus on post-publication integrity concerns related to RCTs. After prospective registration (https://osf.io/njksm), we assembled a multidisciplinary stakeholder group comprising 48 participants from 18 countries across six continents, recruited using a curated list of journal editors and snowballing. An underpinning evidence synthesis collated 89 articles related to post-publication integrity concerns. Integrity statements related to RCTs created were subjected to anonymized two-round Delphi survey. A hybrid face-to-face-online consensus development meeting was convened to consolidate the consensus. The response rates of the two Delphi survey rounds were 65% (31/48) and 67% (32/ 48), respectively. There were 101 and 41 statements in the first and second Delphi rounds, respectively. After the two Delphi rounds and the consensus development meeting, consensus was achieved on 104 statements consolidated to 84 after merging, editing, and removing duplicates. This set of statements included general aspects (n = 9), journal instructions (n = 14), editorial and peer review (n = 7), correspondence and complaints (n = 4), investigations for integrity concerns (n = 16), decisions and sanctions (n = 9), critical appraisal guidance (n = 1), systematic reviews of RCTs (n = 8), and research recommendations (n = 16). In conclusion, this international multistakeholder consensus statement aimed to underpin policies for preventing post-publication integrity concerns in RCT publications and assist in improving investigations of misconduct allegations.
  18. Ang TL, Hang DV, Li JW, Ho JCL, Sy-Janairo ML, Raja Ali RA, et al.
    PMID: 39888113 DOI: 10.1111/jgh.16896
    BACKGROUND AND AIM: The APAGE Position Statements aimed to provide guidance to healthcare practitioners on clinical practices aligned with climate sustainability.

    METHODS: A taskforce convened by APAGE proposed provisional statements. Twenty-two gastroenterologists from the Asian Pacific region participated in online voting and consensus was assessed through an anonymized and iterative Delphi process.

    RESULTS: There were five sections that addressed the rationale for climate action, the importance of adopting principles of waste management, clinical practice, gastrointestinal endoscopy, and issues related to advocacy and research. Sixteen statements achieved consensus and included the following: 1. APAGE recommends adopting prompt measures to reduce the carbon footprint of clinical practice due to the importance of climate action and its health cobenefits. 5. APAGE recommends adherence to professional clinical guidelines to optimize clinical care delivery in gastroenterology and hepatology to avoid the environmental impact of unnecessary procedures and tests. 8. APAGE recommends an emphasis on health promotion, disease prevention, and appropriate screening and surveillance, when resources are available, to reduce the environmental impact of managing more advanced diseases that require more intensive resources. 12. APAGE recommends that technological advances in endoscopic imaging and artificial intelligence, when available, be used to improve the precision of endoscopic diagnosis to reduce the risk of missed lesions and need for unnecessary biopsies. 13. APAGE recommends against the routine use of single-use endoscopes.

    CONCLUSION: The position statements provide guidance to healthcare practitioners on clinical practices in gastroenterology, hepatology, and endoscopy that promote climate sustainability.

  19. Kim D, Lee J, Toyama T, Liyanage T, Woodward M, Matsushita K, et al.
    Nephrology (Carlton), 2025 Feb;30(2):e70002.
    PMID: 39888116 DOI: 10.1111/nep.70002
    AIM: Anaemia is a significant complication of chronic kidney disease (CKD). However, its prevalence and treatment patterns in Asia are poorly understood. We sought to quantify prevalence of anaemia and its treatment in people with CKD across the region.

    METHODS: MEDLINE and Embase (inception to 2023) were systematically searched for observational studies of adults with CKD conducted in Asia that reported the prevalence of anaemia or its treatment. Additional relevant unpublished data were obtained from national experts. Summary estimates of the prevalence of anaemia and its treatment were determined using a random-effects meta-analysis according to country and study-specific CKD inclusion criteria.

    RESULTS: Eighty-six studies from 10 Asian countries reported data on 1 342 121 participants. The overall prevalence of anaemia in individuals with CKD was 42% (95% CI 33%-52%), with wide variation (12%-57% in studies including all CKD stages; 21%-96% in studies limited to individuals with kidney failure). Anaemia prevalence progressively increased with more advanced CKD (80% in Stage 5). Studies reporting data on anaemia treatment, particularly in early CKD, were limited. The prevalence of erythropoietin-stimulating agents (ESAs) and iron therapy was 40% (95% CI 24%-58%) and 21% (95% CI 14%-31%), respectively (ESA: 7%-29% in CKD, 63%-95% in kidney failure; iron: 6%-26% in CKD, 15%-88% in kidney failure).

    CONCLUSION: Our findings indicate a significant, but widely varying, prevalence of anaemia and its treatment in people with CKD in Asia. Substantial variability in data availability and collection highlights the need for standardised reporting to facilitate the development of regionally relevant strategies for anaemia management in CKD.

    MeSH terms: Asia/epidemiology; Humans; Prevalence
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