Browse publications by year: 2025

  1. Syed RU, Moni SS, Hussein W, Alhaidan TMS, Abumilha SMY, Alnahdi LK, et al.
    Sci Rep, 2025 Feb 05;15(1):4369.
    PMID: 39910087 DOI: 10.1038/s41598-025-87319-7
    Cubebin, a dibenzyl butyrolactone lignan belonging to several distinct families, including Aristolochiaceae, Myristicaceae, Piperaceae, and Rutaceae, and possesses several pharmacological activities, including analgesic, anti-inflammatory, antioxidant, and vasodilatory. The current study aimed to evaluate the effect of cubebin on streptozotocin (STZ)-evoked diabetic nephropathy (DN). DN is a well-identified complication of diabetes mellitus (DM) characterized by renal hypertrophy that progressively declines kidney function. Wistar rats were randomly divided into groups- normal, STZ control (65 mg/kg/body weight), and STZ + cubebin (10 and 20 mg/kg). Biochemical parameters such as glucose levels, kidney parameters, lipid profile, oxidative stress, endogenous antioxidant markers, inflammatory cytokines and histopathology were performed. Molecular docking [(PDB ID: TNF-α (7JRA), NF-κB (1SVC), TGF-β1 (3TZM)] and dynamic simulation (MDS) were also performed with the selected target. STZ-induced DN was changes in these parameters. In contrast, DN + cubebin at 10 and 20 mg/kg doses improved the biochemical parameters and histological changes. Furthermore, molecular docking and simulation studies showed a binding affinity with negative binding energy with TNF-α (7jra, - 11.342 kcal/mol), TGF-β1 (3tzm, - 9.162 kcal/mol) and NF-κB (1svc, - 6.665 kcal/mol). The results of MDS provided insight into the mechanisms that associate proteins TNF-α, NF-κB, and TGF-β1 in conformational dynamics upon binding to cubebin. In conclusion, these findings exhibit a potential effect of cubebin in STZ-evoked DN rats.
    MeSH terms: Animals; Kidney/drug effects; Kidney/metabolism; Kidney/pathology; Male; Streptozocin; Signal Transduction/drug effects; Transforming Growth Factor beta/metabolism; Rats, Wistar*; Oxidative Stress/drug effects; Rats; Transforming Growth Factor beta1/metabolism; Molecular Docking Simulation*
  2. Mustafa M, Karobari MI, Al-Maqtari AAA, Abdulwahed A, Almokhatieb AA, Almufleh LS, et al.
    Sci Rep, 2025 Feb 05;15(1):4392.
    PMID: 39910098 DOI: 10.1038/s41598-025-86277-4
    Understanding root canal morphology is essential for successful endodontic treatment. This study aimed to investigate morphological variations in root and canal systems of anterior and premolar teeth across different age groups and genders in a Saudi Arabian subpopulation using a novel classification system. A total of 3573 maxillary and mandibular anterior and premolar teeth in 209 patients were examined using cone-beam computed tomography (CBCT) in a Saudi subpopulation. The number of roots was identified, and root canal configurations were classified according to Ahmed et al. coding system (Int Endod J 50(8):761-770, 2017). Demographic data including gender (male and female) and age (divided into 6 groups-10-20, > 20-30, > 30-40, > 40-50, > 50-60, > 60-70) were recorded. The Chi-square test was used for statistical analysis, and the significance level was set at 0.05 (P = 0.05). Results showed that maxillary and mandibular central incisors, lateral incisors, and canines predominantly exhibited the 1TN1 configuration. Maxillary first premolars had a higher prevalence of double-rooted variants with code 2TN B1 P1. Maxillary second premolars more frequently displayed the 1TN1-2-1 configuration. No significant gender and age differences were observed in maxillary anterior and premolar teeth (p > 0.05). A number of supplemental configurations such as 2TNB1-2P1, 3TN MB1 DB1 P1, and 3TN 1(MB1 DB1) P1 were noted in maxillary premolars. For mandibular anteriors, results showed no significant difference by gender, but for age, mandibular incisors showed more prevalence of complex canal configurations up to 40 years. However, the difference with other age groups (> 40-70 years) was not statistically significant (p  0.05). Several supplemental configurations such as 1TN1-2-3, 1TN1-2-3-2, 2TN B1/L1, and 2TN 1B1/L1) were noted in mandibular first premolars. This study identified a wide range of root and canal anatomical variations in anterior and premolar teeth within a Saudi subpopulation. The findings revealed that while age influenced the complexity of root canal systems in mandibular incisors, no significant association between gender and canal complexity was observed. The Ahmed et al. classification system offered a detailed analysis of these complex variations, providing a clear understanding of the root canal morphology in this population.
    MeSH terms: Adolescent; Adult; Aged; Child; Female; Humans; Incisor/anatomy & histology; Male; Mandible/anatomy & histology; Maxilla/anatomy & histology; Middle Aged; Saudi Arabia; Young Adult
  3. Sodeifian G, Markom M, Mohd Ali J, Mat Salleh MZ, Derakhsheshpour R
    Sci Rep, 2025 Feb 06;15(1):4451.
    PMID: 39910234 DOI: 10.1038/s41598-025-88817-4
    In order to develop supercritical fluid (SCF) processes for the micro or nanosizing of solid solute components, such as pharmaceuticals, it is essential to assess their solubility in solvents including supercritical carbon dioxide (SC-CO2). This crucial step is fundamental for choosing and evaluating the processes in SCF technology. A statistical method was developed and used to determine the solubility of gemcitabine in SC-CO2. The solubility measurements at various pressures and temperatures were conducted using UV-vis analysis. Two model types were used to correlate the data: semi-empirical (with 3-6 parameters) and equation of state (EoS) models. The solubility of gemcitabine in SC-CO2 using a static method at temperatures of 308-338 K and pressures of 120-270 bar were measured and modeled for the first time. Solubility of gemcitabine ranged from 0.1274 × 10- 5 to 1.8128 × 10- 5 in mole fraction and 0.00295 to 0.08489 kg/L. The PR EoS model performed best at 308 K with an AARD of 12.58%, and SRK excelled at 318-338 K with AARDs between 12.93 and 15.68%. Application of the Joback method (AARD 12.04-27.13%) and COSMO-RS method (AARD 18.68-31.28%) in EoS models also were compared. The Bian et al. model showed the best fit among density-based models with an AARD of 16.62%.
    MeSH terms: Antineoplastic Agents/chemistry; Pressure; Solubility*; Temperature; Thermodynamics*
  4. Jing Y, Lee JC, Moon WC, Ng JL, Yew MK, Jin Y
    Sci Rep, 2025 Feb 05;15(1):4352.
    PMID: 39910248 DOI: 10.1038/s41598-025-88927-z
    The environmental issues in the construction industry have garnered considerable attention in numerous studies. Ecologically sustainable green concrete addresses environmental challenges in the construction industry. This study investigates the impact of multi-walled carbon nanotubes (0-0.20%) in rice husk ash (15%) concrete to replace Portland cement. The mechanical and durability properties of four concrete mixtures were analysed. Adding 0.1% multi-walled carbon nanotubes and 15% rice husk ash yielded satisfactory results, significantly improving durability compared to concrete without multi-walled carbon nanotubes. With the addition of 0.1-0.2% multi-walled carbon nanotubes, the density and elastic modulus increased, the 28-d sorptivity decreased by 4.64-28.76%. The resistance ability of 111-d mass loss and compressive strength loss increased by 50.93-61.71% and 25.28-48.47% under sulphate attack, respectively. The resistance ability of mass loss increased by 3.7-35.97% under acid attack. And 120-d drying shrinkage resistance improved by 3.08-9.23%. The predicted and experimental results were compared using the Sakata, GL 2000, B3, ACI 209, and CEB-FIP models. Sakata and B3 provided the most accurate early-stage and long-term drying shrinkages with variation coefficients of 0.13-0.33 and 0-0.05, respectively. Moreover, the sustainability of rice husk ash concrete containing multi-walled carbon nanotubes was evaluated, and its environmental friendliness was confirmed. Thus, the viability of multi-walled carbon nanotubes in rice husk ash sustainable concrete significantly contributes to sustainable construction.
  5. Yu W, Xiao Y, Jayaraman A, Yen YC, Lee HU, Pettersson S, et al.
    EMBO Mol Med, 2025 Feb;17(2):249-264.
    PMID: 39910348 DOI: 10.1038/s44321-024-00179-y
    Changes in gut microbiota composition have been linked to anxiety behavior in rodents. However, the underlying neural circuitry linking microbiota and their metabolites to anxiety behavior remains unknown. Using male C57BL/6J germ-free (GF) mice, not exposed to live microbes, increased anxiety-related behavior was observed correlating with a significant increase in the immediate early c-Fos gene in the basolateral amygdala (BLA). This phenomenon coincided with increased intrinsic excitability and spontaneous synaptic activity of BLA pyramidal neurons associated with reduced small conductance calcium-activated potassium (SK) channel currents. Importantly, colonizing GF mice to live microbes or the microbial-derived metabolite indoles reverted SK channel activities in BLA pyramidal neurons and reduced the anxiety behavioral phenotype. These results are consistent with a molecular mechanism by which microbes and or microbial-derived indoles, regulate functional changes in the BLA neurons. Moreover, this microbe metabolite regulation of anxiety links these results to ancient evolutionarily conserved defense mechanisms associated with anxiety-related behaviors in mammals.
    MeSH terms: Gastrointestinal Microbiome/physiology; Amygdala/metabolism; Animals; Behavior, Animal; Indoles/metabolism; Male; Mice, Inbred C57BL*; Neurons/metabolism; Proto-Oncogene Proteins c-fos/metabolism; Pyramidal Cells/metabolism; Mice; Small-Conductance Calcium-Activated Potassium Channels/metabolism; Basolateral Nuclear Complex/metabolism
  6. Bazina WK, Tawfik WA, Abd Elghany NA, Saadony S, Abdul Kari Z, Alamoudi MO, et al.
    BMC Vet Res, 2025 Feb 05;21(1):50.
    PMID: 39910528 DOI: 10.1186/s12917-025-04471-y
    BACKGROUND: This study aimed to evaluate the effects of selenium nanoparticles (SeNPs) and/or vitamin E (VE) on the growth, body composition, metabolic parameters, histopathology, and resistance of Nile tilapia to Aspergillus flavus.

    RESULTS: Monosex Nile tilapia fingerlings were sourced from the Bazina farm and hatchery in Ismailia Governorate, Egypt, where the experiment was also conducted. The fish were acclimatized for 15 days before the trial. A total of 240 fingerlings (average weight 46 ± 3.0 g/fish) were divided equally across 12 concrete tanks (1 × 1 × 1.2 m, 1 m³ capacity), with 20 fish per tank. The fish were fed a control diet (T0), which was a basal diet with no supplementation, or one of three experimental diets for 60 days: T1 (1 mg SeNPs/kg), T2 (100 mg VE/kg), and T3 (1 mg SeNPs + 100 mg VE/kg). The experiment followed a completely randomized design (CRD) with three replicates per treatment. The combination of SeNPs and VE (T3) resulted in the best feed conversion ratio. A slight but significant increase (P 

    MeSH terms: Animals; Aspergillosis/drug therapy; Aspergillosis/veterinary; Body Composition/drug effects; Dietary Supplements*; Nanoparticles/administration & dosage; Disease Resistance/drug effects
  7. Huang WX, Chong MC, Tang LY, Liu XX, Fang M, Shen YY, et al.
    BMC Nurs, 2025 Feb 05;24(1):135.
    PMID: 39910581 DOI: 10.1186/s12912-024-02528-1
    BACKGROUND: Nurses play a critical role in the implementation of child-friendly healthcare, ensuring that pediatric patients receive comprehensive and compassionate services. However, there is a global scarcity of research evaluating nurses' practices in delivering child-friendly healthcare. Therefore, this study focuses on exploring the assessment of child-friendly healthcare practices from the perspective of nurses themselves.

    METHOD: This descriptive qualitative study employed purposive and snowball sampling methods to recruit 14 pediatric nurses from six hospitals in Zhejiang province, Southeast China. Individual semi-structured interviews were conducted face-to-face between January and June 2024. Data were analyzed through the qualitative content analysis approach.

    RESULTS: We identified 415 unique meaning units in the interview data, 332 of which were related to child-friendly healthcare practice assessment. These units were classified into 58 codes across 4 categories and 17 subcategories. The four categories were identified: services designed for children's interests, tailoring the environment adapted for children, facilitating social interactions, and promoting childhood development.

    CONCLUSION: The findings from this study were derived from the perspectives of Chinese nurses on child-friendly healthcare practice assessment, offering a comprehensive and multidimensional view of this field. The results reflect the current state and future direction of child-friendly healthcare in China, which will aid in sustaining the development of a child-friendly healthcare evaluation system.

  8. Heng W, Ho MH, Mah XH, Lim JJ, Syamsury NHBA, Haagensen E, et al.
    Med Educ, 2025 Feb 05.
    PMID: 39910710 DOI: 10.1111/medu.15611
    BACKGROUND: Active learning is a learning process that promotes student engagement in constructing knowledge and conceptual understanding, improves critical thinking skills and develops professional competency. In recent years there has been a significant shift of emphasis in higher education from passive teacher-centred didactic teaching to active student-led learning. Although there is abundant literature about active learning, there is a gap in the knowledge of students' perception regarding factors that affect engagement in active learning activities. This project aimed to explore pre-clinical year medical students' perception of active learning and examine the factors that affect their participation in active learning activities.

    METHODS: A mixed-method study was conducted with pre-clinical medical students at Newcastle University Medical School, UK, and Newcastle University Medicine Malaysia. A total of 266 students participated in an online survey questionnaire, with 25 students participating in focus group discussions (FGD). Quantitative data were analysed using descriptive analysis and qualitative data was analysed with thematic analysis.

    RESULTS: The majority of students (94.7%) recognised that active learning is important for their learning, but had a narrow definition of what active learning constituted, and familiarity with active learning techniques was lacking. Many students' independent learning techniques were centred around methods of 'active recall', with factors affecting the utilisation of active learning techniques mainly focused on time availability, group dynamics in active teaching sessions and teaching styles of educators.

    CONCLUSION: Students acknowledged the importance of active learning but are generally unfamiliar with ways to effectively utilise a broad range of active learning strategies. This study demonstrated that it is important for educators to understand firstly how students define active learning as well as how students interact with active learning taught sessions, to ensure that they create an environment where students feel confident to engage in active learning techniques.

  9. Tan FY, Mohd Nor NAB, Ahmad SF, Hariri F
    PMID: 39910968 DOI: 10.1177/10556656251319642
    BACKGROUND: Conventionally, vector guidance for internal distractor placement during cleft maxillary distraction osteogenesis (DO) is done using vector guidance splint. Vector guidance splint cannot be fabricated if occlusion is not decompensated for model surgery.

    SOLUTION: Using mandibular occlusal plane as anatomical reference, vector guidance oral prosthesis is fabricated to guide distractor placement and vector determination, in cases where model surgery is not possible.

    WHAT WE DO THAT IS NEW: Vector guidance oral prosthesis is a new prototype produced to guide maxillary DO without the need of model surgery.

  10. Li K, Omar Dev RD, Li W
    Front Public Health, 2025;13:1544194.
    PMID: 39911221 DOI: 10.3389/fpubh.2025.1544194
    INTRODUCTION: This study explores the role of physical activity, exercise attitude, and sleep quality in college students' happiness, aiming to provide a theoretical basis for improving college students' mental health.

    METHODS: The study employed a whole group sampling method, utilizing a physical activity level scale, exercise attitude scale, sleep quality scale, and happiness scale. A questionnaire survey was conducted with 1,308 college students from four universities in China.

    RESULTS: The analysis yielded three key findings: (1) Physical activity significantly improves college students' happiness, and this improvement can be achieved through direct and indirect effects. (2) Positive exercise attitude plays an important mediating role between physical activity and happiness, and a high level of exercise attitude can enhance happiness. In addition, the effect of physical activity on happiness is most significant through exercise attitude. (3) Although sleep quality did not significantly mediate the relationship between physical activity and happiness alone, it played a positive role in the chain mediation path of "physical activity → exercise attitude → sleep quality → happiness."

    DISCUSSION: The discovery of the chain mediation path shows that physical activity not only affects happiness, but also indirectly affects college students' happiness through the combined effects of psychological and physiological factors.

    MeSH terms: Adolescent; Adult; Attitude; China; Female; Happiness*; Humans; Male; Surveys and Questionnaires; Universities; Young Adult
  11. Izadiyan Z, Misran M, Kalantari K, Webster TJ, Kia P, Basrowi NA, et al.
    Int J Nanomedicine, 2025;20:1213-1262.
    PMID: 39911259 DOI: 10.2147/IJN.S488961
    Liposomal nanomedicines have emerged as a pivotal approach for the treatment of various diseases, notably cancer and infectious diseases. This manuscript provides an in-depth review of recent advancements in liposomal formulations, highlighting their composition, targeted delivery strategies, and mechanisms of action. We explore the evolution of liposomal products currently in clinical trials, emphasizing their potential in addressing diverse medical challenges. The integration of immunotherapeutic agents within liposomes marks a paradigm shift, enabling the design of 'immuno-modulatory hubs' capable of orchestrating precise immune responses while facilitating theranostic applications. The recent COVID-19 pandemic has accelerated research in liposomal-based vaccines and antiviral therapies, underscoring the need for improved delivery mechanisms to overcome challenges like rapid clearance and organ toxicity. Furthermore, we discuss the potential of "smart" liposomes, which can respond to specific disease microenvironments, enhancing treatment efficacy and precision. The integration of artificial intelligence and machine learning in optimizing liposomal designs promises to revolutionize personalized medicine, paving the way for innovative strategies in disease detection and therapeutic interventions. This comprehensive review underscores the significance of ongoing research in liposomal technologies, with implications for future clinical applications and enhanced patient outcomes.
    MeSH terms: Humans; Immunotherapy/methods; Neoplasms/drug therapy; Drug Delivery Systems/methods
  12. Muthukrishnan S, Tey DKH, Wee WC
    Data Brief, 2025 Feb;58:111309.
    PMID: 39911272 DOI: 10.1016/j.dib.2025.111309
    The dataset demonstrates the efficacy of Aqua Ectogon-284, a zinc chelates of glycine, as a dietary supplement to enhance growth and survival in hybrid grouper (Epinephelus fuscoguttatus ♀ × E. lanceolatus ♂) and whiteleg shrimp (Penaeus vannamei). Two concentrations (1g/kg feed and 2g/kg feed) were evaluated against a control group to identify an optimal dosage that maximizes growth and health outcomes. Both the species exhibited improved growth performance and survival rates with dietary supplementation. The hybrid grouper fed a 1g/kg diet achieved the highest final weight of 100.00 g, with significant increases in length and survival rates of 96.00%. Similarly, whiteleg shrimp demonstrated enhanced final weights and survival (94.67%) with the same treatment. These findings revealed that zinc chelate glycine increased the growth rate and reduced mortality in farmed species.
  13. Khairi Wazien Pengiran Umar A, Goh HP, Rizidah Murang Z, Hermansyah A, Rehman IU, Goh KW, et al.
    J Pharm Policy Pract, 2025;18(1):2426137.
    PMID: 39911586 DOI: 10.1080/20523211.2024.2426137
    BACKGROUND: Brunei Darussalam's medicine control governance landscape is complex, driven by legislative directives and directed by the Brunei Darussalam Medicines Control Authority (BDMCA). While the medicine dispensing system in Brunei Darussalam, across both governmental and private sectors, is a critical component of the nation's healthcare, it remains under-researched, leaving gaps in understanding its distinctions, challenges, and opportunities. This study aimed to explore deep into the perspectives of pharmacists regarding the medicine dispensing systems in Brunei Darussalam, medicine accessibility, the classification and regulation of medicines, and the growing role of pharmacists.

    METHODS: Using a narrative qualitative approach, we conducted in-depth one-on-one interviews with six pharmacists, delving into their personal experiences with topics such as medicine classification and the broader systems for medication provision. The collected narratives were analysed through a rigorous thematic analysis, following the framework established by Braun & Clarke.

    RESULTS: Five themes emerged from the data: nuances of Brunei Darussalam's medicine dispensing landscape across both public and private sectors; accessibility and regulatory framework of medicines both in Brunei Darussalam and abroad; prospective implementations to enhance the current system; and the evolving role and expectations of pharmacists in the current healthcare environment. Notably, a significant contribution of this study was the illumination of the differential perspectives between pharmacists operating in governmental versus private contexts.

    CONCLUSION: This study sheds light on the previously under-explored domain of Brunei Darussalam's medicine control governance and medicine dispensing system. By drawing from the first-hand experiences of practicing pharmacists, it offers actionable insights that could guide future policy developments, optimise medicine dispensation and regulation, and shape the evolving role of pharmacists in Brunei Darussalam.

  14. Hussain R, Loh ZC, Ababneh BF, Muneswarao J, Ong SC, Saini B, et al.
    J Pharm Policy Pract, 2025;18(1):2455065.
    PMID: 39911587 DOI: 10.1080/20523211.2025.2455065
    BACKGROUND: Short-acting beta-2 agonists (SABA) are bronchodilators that offer rapid relief for asthma patients experiencing acute symptoms. The availability of SABA inhalers without a prescription may exacerbate the overuse of SABA. This study aimed to explore the perceptions, practices, and experiences of asthma patients and community pharmacists toward using SABA inhalers in Malaysia.

    METHODS: A qualitative study was conducted among eleven asthma patients and twelve community pharmacists using semi-structured individual interviews. All interviews were audio-recorded and transcribed verbatim and then analyzed by thematic analysis. The findings were reported using the COREQ checklist.

    RESULTS: Thematic analysis yielded eight major themes, (1) perceptions and understanding of good asthma control; (2) perceptions and experience towards asthma follow-up review; (3) perceptions of SABA's reliance; (4) practices towards the proper use of inhalers; (5) over-the-counter availability of SABA inhalers; (6) provision of pharmacists in SABA use assessment; (7) patients-healthcare professionals communication; and (8) recommendations for policymakers.

    CONCLUSION: Most asthma patients and community pharmacists agreed that good asthma control was associated with reduced SABA usage and minimum asthma symptoms. The majority of patients claimed that visiting physicians for asthma follow-up reviews was unnecessary. Moreover, community pharmacists have raised concerns regarding patients' reliance on SABA inhalers due to the immediate relief effects, however, the majority of interviewed patients claimed that they did not rely on SABA inhalers. Additionally, community pharmacists were generally concerned about the frequency, dosage, and techniques using SABA inhalers. These concerns need to be addressed to improve the safe use of SABA inhalers. Language barriers, health literacy, long waiting times, and public education were the most important believed determinants of the safe use of SABA inhalers.

  15. Paneerselvam GS, Kah Yee C, Farrukh MJ, Yuda A, Hermansyah A, Mohd Asmani MF, et al.
    J Pharm Policy Pract, 2025;18(1):2446912.
    PMID: 39911588 DOI: 10.1080/20523211.2024.2446912
    BACKGROUND: Medication-related problems (DRPs) are common among hemodialysis (HD) patients, and pharmacist-led medication reviews have been shown to address such issues. However, the impact of these interventions and the specific types of DRPs among this patient group remain unclear.

    OBJECTIVES: This systematic review aimed to assess the impact of pharmacist-led medication reviews among HD patients, identify the most prevalent types of DRPs, and explore the factors associated with these problems.

    METHODS: A systematic search was conducted across databases such as Medline via PubMed, Science Direct, Google Scholar, and EBSCOHost, for studies published from January 2012 to July 2023. Studies included were those focusing on pharmacist interventions in HD patients. The Newcastle-Ottawa Scale (NOS) was used to evaluate the quality of selected studies.

    RESULTS: After screening 343 articles, 10 studies (involving 1342 HD patients) were included. Nine studies were rated as high quality, and one as fair quality. The studies predominantly used prospective designs. A total of 4511 DRPs were identified, with suboptimal drug treatment, non-adherence to medications, and drug use without indication being the most common issues. Pharmacist interventions led to the resolution or reduction of DRPs, shorter hospital stays, improvement in laboratory outcomes, better quality of life (QoL), and enhanced patient understanding. However, interventions had minimal or no significant impact on reducing unplanned admissions, mortality rates, or improving medication adherence. The reduction in healthcare utilisation costs was inconsistent across studies.

    CONCLUSION: Pharmacist-led medication reviews were effective in resolving DRPs and improving clinical outcomes in HD patients, such as quality of life and lab values. However, their impact on healthcare utilisation and mortality remains inconclusive. Further research with longer follow-up is needed to assess the long-term economic outcomes of these interventions.

  16. Lee LH, Ho CSH, Chan YL, Tay GWN, Lu CK, Tang TB
    PMID: 39911775 DOI: 10.1109/JTEHM.2024.3506556
    While functional near-infrared spectroscopy (fNIRS) had previously been suggested for major depressive disorder (MDD) diagnosis, the clinical application to predict antidepressant treatment response (ATR) is still unclear. To address this, the aim of the current study is to investigate MDD ATR at three response levels using fNIRS and micro-ribonucleic acids (miRNAs). Our proposed algorithm includes a custom inter-subject variability reduction based on the principal component analysis (PCA). The principal components of extracted features are first identified for non-responders' group. The first few components that sum up to 99% of explained variance are discarded to minimize inter-subject variability while the remaining projection vectors are applied on all response groups (24 non-responders, 15 partial-responders, 13 responders) to obtain their relative projections in feature space. The entire algorithm achieved a better performance through the radial basis function (RBF) support vector machine (SVM), with 82.70% accuracy, 78.44% sensitivity, 86.15% precision, and 91.02% specificity, respectively, when compared with conventional machine learning approaches that combine clinical, sociodemographic and genetic information as the predictor. The performance of the proposed custom algorithm suggests the prediction of ATR can be improved with multiple features sources, provided that the inter-subject variability is properly addressed, and can be an effective tool for clinical decision support system in MDD ATR prediction. Clinical and Translational Impact Statement-The fusion of neuroimaging fNIRS features and miRNA profiles significantly enhances the prediction accuracy of MDD ATR. The minimally required features also make the personalized medicine more practical and realizable.
    MeSH terms: Adult; Algorithms; Female; Humans; Male; Middle Aged; Treatment Outcome; Principal Component Analysis; MicroRNAs*; Young Adult; Support Vector Machine*
  17. Davias A, Verghese M, Bridgman SL, Tun HM, Field CJ, Hicks M, et al.
    Brain Behav Immun Health, 2025 Mar;44:100946.
    PMID: 39911944 DOI: 10.1016/j.bbih.2025.100946
    BACKGROUND: Dysbiosis of the gut microbiota has been demonstrated in neurodevelopmental disorders but the underlying mechanisms that may explain these associations are poorly understood. Gut secretory immunoglobulin A (SIgA) binds pathogenic microbes, preventing mucosal penetration. Gut microbes also influence SIgA production and its binding characteristics through short-chain fatty acid (SCFA) metabolites, allowing them to regulate the immune response. Serum IgA deficiency has been noted in children with autism spectrum disorders (ASD). In this study, we aimed to determine whether SIgA level in infancy is associated with gut microbiota taxonomy and metabolites, and neurodevelopmental outcomes in preschool children.

    METHODS: For a subsample of 178 children from the Canadian CHILD Cohort Study, gut microbiota of fecal samples collected at 3-4 months and 12 months was profiled using 16S rRNA sequencing. Gut bacterial metabolites levels and SIgA level were measured by nuclear magnetic resonance (NMR) based metabolomics and SIgA enzyme-linked immunosorbent assay at 3-4 months, respectively. Bayley-III Scale of Infant Development was assessed at 12 and 24 months. We evaluated direct relationships in multiple linear regression models and putative causal relationships in statistical mediation models.

    RESULTS: Propionate and butyrate levels at 3-4 months were associated with decreased Bayley cognitive score at 24 months (p-values: 0.01 and 0.02, respectively) in adjusted multiple linear regression models, but when we investigated an indirect relationship mediated by decreased SIgA level at 3-4 months, it did not reach statistical significance (p-values: 0.18 and 0.20, respectively). Lactate level at 3-4 months was associated with increased Bayley cognitive score at 24 months in adjusted multiple linear regression models (p-value: 0.01), but the statistical model mediated by increased SIgA level at 3-4 months did not reach statistical significance neither (p-value: 0.20).

    CONCLUSIONS: Our study contributes to growing evidence that neurodevelopment is influenced by the infant gut microbiota and that it might involve SIgA level, but larger studies are required.

  18. Verma SB, Khurana A, Bosshard PP, Kargl A, Singal A, Saraswat A, et al.
    PMID: 39912159 DOI: 10.25259/IJDVL_1793_2024
  19. Yong TL, Zaman R, Rehman N, Tan CK
    Exp Dermatol, 2025 Feb;34(2):e70042.
    PMID: 39912256 DOI: 10.1111/exd.70042
    Ceramide has transitioned from an incidental discovery to a vital element in skincare, becoming a thoroughly studied compound in the quest to treat skin conditions. Creating a moisture barrier, preserving hydration, regulating pH, controlling inflammation, and enhancing skin functions and appearance are among its established benefits. It is often used medically to repair skin barrier defects, as observed in inflammatory skin conditions like atopic dermatitis (AD) and dry skin types. Furthermore, ceramide and its metabolites are commonly used as predictors before disease manifestation and for prognostication processes, thus can be used as biomarker for clinical diagnosis as well. In the last couple of decades, momentum was also seen in the pre-clinical studies involving anti-cancer and nanotechnology field, whereby ceramide was also used as a drug, a carrier, or even adjunct formulation to increase efficacy of treatment such as chemotherapy. Approaches to increase ceramide levels include directly replenishing lost ceramides with natural extracts, synthetic pseudo-ceramides, or ceramide-like analogues, as well as using supplements that stimulate the body's natural ceramide production. Although ceramide is a well-known treatment in skincare and for common skin conditions like AD and psoriasis, its development and related pharmacology for severe skin conditions, such as skin cancer, remain in pre-clinical stages. Hence, the purpose of this research is to explore the role of ceramide in skin health and its application in common skin diseases.
    MeSH terms: Animals; Dermatitis, Atopic/drug therapy; Humans; Psoriasis/drug therapy; Psoriasis/metabolism; Skin Diseases/drug therapy; Skin Neoplasms/metabolism; Skin Physiological Phenomena
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