Browse publications by year: 2025

  1. Mohammad Basir MF, Ibrahim MI, Wan Mohamad WMZ, Mohd Fuzi NMH, Rosli AS, Abdul Rahman MS, et al.
    One Health Outlook, 2025 Mar 18;7(1):16.
    PMID: 40098000 DOI: 10.1186/s42522-025-00137-9
    BACKGROUND: Rabies poses a significant public health threat worldwide, primarily transmitted through dog bites. Understanding community knowledge, attitudes, and practices (KAP) regarding rabies is crucial for effective prevention and control strategies. This study aimed to determine KAP levels and their determinants among dog owners in Kelantan, Malaysia.

    METHODS: A cross-sectional study was conducted in 2023 among 232 dog owners attending mass canine vaccination programs conducted by Kelantan State Department of Veterinary Services. A validated and structured questionnaire assessed knowledge, attitudes and practices of rabies. The determinants were analysed through multiple logistic regression analyses.

    RESULTS: Overall, 53.0% exhibited good knowledge, 26.7% had positive attitudes, and 40.0% demonstrated good practices concerning rabies. Higher education levels (AOR = 3.15,95%CI:1.07,9.26), attending rabies programs (AOR = 3.79,95%CI:1.37,10.43), and owning two dogs (AOR = 2.25,95%CI:1.09,4.61) were associated with good knowledge. Medium and long duration of dog ownership durations (AOR = 0.23,95%CI:0.10,0.53 and AOR = 0.40, 95%CI:0.16,0.98 respectively) has lower chances to have positive attitudes, while larger households (AOR = 1.49,95%CI:1.18,1.87) and higher knowledge scores (AOR = 2.80,95% CI:1.06,7.42) were associated to positive attitudes. Older age groups (AOR = 0.27,95%CI:0.12,0.6 and AOR = 0.22,95%CI:0.09,0.50) was associated with suboptimal practices, whereas higher knowledge scores (AOR = 1.29,95% CI:1.08,1.54) and acquiring dogs through formal channels (AOR = 4.79,95%CI:1.85,12.39) were associated with good practices.

    CONCLUSION: The study identified critical knowledge gaps, misconceptions, and suboptimal practices among dog owners in Kelantan, highlighting the need for targeted interventions. Tailored educational programs, community engagement strategies, and a comprehensive One Health approach towards the high-risk groups of dog owner are recommended to enhance rabies prevention and control efforts, ultimately safeguarding human, and animal health.

  2. Teh XR, Looareesuwan P, Pattanaprateep O, Pattanateepapon A, Attia J, Thakkinstian A
    BMC Med Inform Decis Mak, 2025 Mar 17;25(1):134.
    PMID: 40098113 DOI: 10.1186/s12911-025-02964-2
    BACKGROUND: Identification of prognostic factors for diabetes complications are crucial. Glucose variability (GV) and its association with diabetes have been studied extensively but the inclusion of measures of glucose variability (GVs) in prognostic models is largely lacking. This study aims to assess which GVs (i.e., coefficient of variation (CV), standard deviation (SD), and time-varying) are better in predicting diabetic complications, including cardiovascular disease (CVD), diabetic retinopathy (DR), and chronic kidney disease (CKD). The model performance between traditional statistical models (adjusting for covariates) and machine learning (ML) models were compared.

    METHODS: A retrospective cohort of type 2 diabetes (T2D) patients between 2010 and 2019 in Ramathibodi Hospital was created. Complete case analyses were used. Three GVs using HbA1c and fasting plasma glucose (FPG) were considered including CV, SD, and time-varying. Cox proportional hazard regression, ML random survival forest (RSF) and left-truncated, right-censored (LTRC) survival forest were compared in two different data formats (baseline and longitudinal datasets). Adjusted hazard ratios with 95% confidence intervals were used to report the association between three GVs and diabetes complications. Model performance was evaluated using C-statistics along with feature importance in ML models.

    RESULTS: A total of 40,662 T2D patients, mostly female (61.7%), with mean age of 57.2 years were included. After adjusting for covariates, HbA1c-CV, HbA1c-SD, FPG-CV and FPG-SD were all associated with CVD, DR and CKD, whereas time-varying HbA1c and FPG were associated with DR and CKD only. The CPH and RSF for DR (C-indices: 0.748-0.758 and 0.774-0.787) and CKD models (C-indices: 0.734-0.750 and 0.724-0.740) had modestly better performance than CVD models (C-indices: 0.703-0.730 and 0.698-0.727). Based on RSF feature importance, FPG GV measures ranked higher than HbA1c GV, and both GVs were the most important for DR prediction. Both traditional and ML models had similar performance.

    CONCLUSIONS: We found that GVs based on HbA1c and FPG had comparable performance. Thus, FPG GV may be used as a potential monitoring parameter when HbA1c is unavailable or less accessible.

    MeSH terms: Machine Learning; Adult; Aged; Blood Glucose*; Diabetes Mellitus, Type 2*; Female; Hemoglobin A, Glycosylated/analysis; Humans; Male; Middle Aged; Prognosis; Retrospective Studies; Diabetes Complications/blood
  3. Singh VA, Phinder HS, Haseeb A, Santharalinggam RD, Yasin NF
    J Orthop Surg (Hong Kong), 2025;33(1):10225536251326059.
    PMID: 40098258 DOI: 10.1177/10225536251326059
    IntroductionEndoprosthetic reconstruction is a widely used technique; however, it is associated with various postoperative complications. One often overlooked complication is the alteration of foot loading patterns. This pilot study evaluated functional outcomes with postoperative static foot loading patterns in patients undergoing endoprosthesis replacements compared to the contralateral limb.ObjectiveThis study evaluates static foot loading patterns in patients undergoing endoprosthetic replacements. It explores the relationship between the functional outcomes of the operated limb and foot loading patterns in a standing position. Additionally, the study considers factors such as the anatomical location of the endoprosthetic reconstruction, limb length discrepancy, age, gender, and body mass index (BMI).Materials and MethodsThis prospective cross-sectional study included 100 patients over 15 years who had undergone lower limb endoprosthetic reconstruction at least 1 year prior and could walk unaided. Data collection encompassed patient demographics, diagnosis, anthropometric measurements, postoperative functional status (evaluated using AOFAS-Hindfoot, MSTS, and TESS scores), and postoperatively analysis of foot loading patterns.ResultsThe patient group has a significantly higher prevalence of abnormal foot arches compared to the expected population proportion. Notably, 10% of patients transitioned from a normal arch to flatfoot, while 40% developed a high arch post-surgery. Asymmetrical arches were observed in 30 patients. Post-surgery, 18.5% of patients experienced a transition from a normal foot arch to a high arch, and 4.6% transitioned to flatfoot. Analysis of variables such as surgical site, limb length discrepancy, age, gender, BMI, foot loading patterns and functional scores (MSTS, TESS and AOFAS) showed no significant associations with these changes.ConclusionAlthough no significant correlation was identified between functional scores and foot loading patterns following lower limb endoprosthetic surgery, a notable prevalence of abnormal foot arches was observed. We recommend routine podiatry consultations for these patients to address and manage potential foot-related complications.
    MeSH terms: Adult; Aged; Cross-Sectional Studies; Female; Flatfoot/physiopathology; Flatfoot/surgery; Foot/physiopathology; Foot/surgery; Humans; Male; Middle Aged; Pilot Projects; Prospective Studies; Weight-Bearing/physiology; Treatment Outcome; Lower Extremity/surgery
  4. Chong CSC, Lau YY, Michels PAM, Lim CSY
    Crit Rev Microbiol, 2025 Mar 17.
    PMID: 40098357 DOI: 10.1080/1040841X.2025.2473332
    The rise of antibiotic-resistant bacteria poses a grave threat to global health, with the ESKAPE pathogens, which comprise Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa and Enterobacter spp. being among the most notorious. The World Health Organization has reserved a group of last-resort antibiotics for treating multidrug-resistant bacterial infections, including those caused by ESKAPE pathogens. This situation calls for a comprehensive understanding of the resistance mechanisms as it threatens public health and hinder progress toward the Sustainable Development Goal (SDG) 3: Good Health and Well-being. The present article reviews resistance mechanisms, focusing on emerging resistance mutations in multidrug-resistant ESKAPE pathogens, particularly against last-resort antibiotics, and describes the role of biofilm formation in multidrug-resistant ESKAPE pathogens. It discusses the latest therapeutic advances, including the use of antimicrobial peptides and CRISPR-Cas systems, and the modulation of quorum sensing and iron homeostasis, which offer promising strategies for countering resistance. The integration of CRISPR-based tools and biofilm-targeted approaches provides a potential framework for managing ESKAPE infections. By highlighting the spread of current resistance mutations and biofilm-targeted approaches, the review aims to contribute significantly to advancing our understanding and strategies in combatting this pressing global health challenge.
  5. Fan K, Lin C, Li L, Huang Q, Dai J, Wang P, et al.
    Environ Sci Technol, 2025 Mar 18.
    PMID: 40098442 DOI: 10.1021/acs.est.5c00233
    It has been previously observed that rainwater input into paddy rice soils reduced the level of grain-borne arsenic, and it is hypothesized that a Fenton-like reaction triggered by interaction between rainwater-borne hydrogen peroxide and ferrous iron in paddy soils is responsible for microbially mediated impediment of As uptake by rice plants. However, this hypothesis remains untested. This study tested the hypothesis through mesocosm experiments, confirming that rainwater-borne hydrogen peroxide triggered hydroxyl radical (•OH) generation, elevating soil redox potential, and oxidizing arsenite to less phytoavailable arsenate in soil porewater, thereby reducing As uptake by rice and As accumulation in rice grain. Comparison between two crops of rice cultivation with different fluxes of rainwater-borne hydrogen peroxide confirms that seasonal rainfall variation has an impact on accumulation of rice grain-borne arsenic, with paddy soil receiving more rainfall having a lower arsenic concentration in the rice grain compared to that receiving less rainfall. Using China's major rice-producing region as an example, it is demonstrated that spatial variation in rainfall regime could impact the geographical distribution of rice grain-borne As at a national scale. The findings have implications for the assessment and management of the environmental risk from arsenic-contaminated rice grains.
  6. Jeyanthi V, Dhanasekaran J, Joseph SJ, Bajaber MA, Velusamy P, Anbu P, et al.
    APMIS, 2025 Mar;133(3):e70018.
    PMID: 40098601 DOI: 10.1111/apm.70018
    The global rise in antimicrobial resistance poses significant challenges to treating infectious diseases, particularly those caused by methicillin-resistant Staphylococcus aureus (MRSA) in healthcare settings. This research explores the potential of halophilic microorganisms as a source of novel antimicrobial compounds, focusing on Bacillus safensis isolated from saltpan soils in the Tuticorin coastal region, India. Among 158 isolates, B. safensis strain TC67 demonstrated potent anti-MRSA activity and was optimized under specific growth conditions for maximal metabolite production. The active compound was purified through silica gel column chromatography and analyzed using TLC, GC-MS, and 1H NMR spectroscopy. These analyses identified benzene derivatives and saturated fatty acids as key components, including eicosanoic and decanoic acids. The purified metabolites exhibited a minimum inhibitory concentration (MIC) of 31.25 μg/mL against MRSA. Mechanistic studies using flow cytometry and scanning electron microscopy (SEM) confirmed that the compound disrupts MRSA cell membranes, leading to decreased cell viability. This study highlights the potential of marine-derived Bacillus species as a source for antimicrobial agents, providing viable choices to combat MRSA infections.
    MeSH terms: Benzene Derivatives/isolation & purification; Benzene Derivatives/pharmacology; Benzene Derivatives/chemistry; Chromatography, Thin Layer; India; Gas Chromatography-Mass Spectrometry; Microbial Sensitivity Tests*; Soil Microbiology; Microbial Viability/drug effects
  7. Quan W, Fazlin Zulkifli H, Saari N, Shueb RH, Mustaffa N
    Front Pharmacol, 2025;16:1502931.
    PMID: 40098625 DOI: 10.3389/fphar.2025.1502931
    PURPOSE: Diverse novel therapeutic options for hepatocellular carcinoma (HCC) have surfaced in recent years. However, it is increasingly difficult to select the optimal medication. This research aims to assess overall survival (OS), progression-free survival (PFS), objective response rate (ORR), disease control rate (DCR), adverse events (AEs), and severe adverse events (SAEs) in HCC patients receiving adjuvant therapies compared to those receiving sorafenib.

    METHODS: Four databases were used to search articles. Only randomized controlled trials were included. Indicators such as OS, PFS, DCR, ORR, AEs and SAEs were used as outcomes. The protocol for this meta-analysis was registered with PROSPERO (Registration ID: CRD42024544394).

    RESULTS: Forty trials were included in this meta-analysis. The Oxaliplatin, Fluorouracil, and Leucovorin (OFL) + sorafenib group and the sintilimab + bevacizumab biosimilar group decreased the risk of death and increased PFS, ORR, and DCR. Yet, they also yielded remarkable adverse effects and severe adverse effects. To sum up, the atezolizumab + bevacizumab combination and tepotinib were recommended due to their favorable performance on all indexes.

    CONCLUSION: This study further substantiates the efficacy of combination therapies in HCC, while they cause more toxicity in general. It is pressingly urgent to develop new drugs for liver cancer and find rational strategies to alleviate AEs.

    SYSTEMATIC REVIEW REGISTRATION: PROSPERO, identifier CRD42024544394.

  8. Yassin MA, AlRasheed M, Al-Eisa T, Alhashim N, Alsayegh F, Abouzeid TE, et al.
    Front Nutr, 2025;12:1434484.
    PMID: 40098741 DOI: 10.3389/fnut.2025.1434484
    INTRODUCTION: In recent years, significant advances have been made in the treatment of immune thrombocytopenia (ITP) with the development of thrombopoietin receptor agonists (TPO-RAs). TPO-RAs are often used following the failure of prior therapies or when bleeding episodes persist despite glucocorticoid use. In Muslim countries, where religious observance includes 16/8 intermittent fasting, the timing of medication administration may be affected. This study is the first to evaluate the impact of Ramadan fasting on patients receiving different TPO-RAs.

    METHODS: A multicenter mixed-design study was performed in which Muslim patients who fasted during Ramadan while receiving TPO-RAs were interviewed between 2015 and 2023. Patient responses before, during, and after Ramadan were evaluated retrospectively. The bleeding tendency was assessed as (1) no bleeding, (2) minor cutaneous/mucosal bleeding, or (3) severe bleeding that involves major organs.

    RESULTS: The present study included 100 patients from three Muslim countries, including Qatar, Kuwait, and Saudi Arabia, across four tertiary centers. A complete response was observed in 63% of patients on ROM, 46% on ELT and 37% on AVA. For AVA, the mean platelet (PLT) count before Ramadan was estimated at [146.11 ± 111.76], while during Ramadan, it dropped to [131.7 ± 107.6]. For patients on ELT, the mean PLT count before Ramadan was estimated at [120.02 ± 59.7], while during Ramadan, it dropped to [100.8 ± 68.16] (p = 0.016). For patients on ROM, the mean platelet count before Ramadan was estimated at [122.68 ± 80.57], while during Ramadan, it was [130.94 ± 84.96]. Only 3% (3 patients on ELT) experienced bleeding episodes.

    CONCLUSION: This study supports the feasibility of Ramadan fasting for ITP patients receiving TPO-RAs. Further studies with a larger sample size are recommended to investigate the impact of other types of fasting on the efficacy and safety of TPO-RAs.

  9. Younis S, Jami H, Riaz F, Saleh W
    J Child Adolesc Trauma, 2025 Mar;18(1):151-165.
    PMID: 40098780 DOI: 10.1007/s40653-024-00675-w
    The escalating indirect exposure to terrorism through media has contributed to rising mental health issues among adolescents. The terrorist attack on Army Public School Peshawar (APSP) in Pakistan has received immense media coverage and the violence shown has touched the lives of natives. The current study adopts an Interpretative phenomenological analysis to explore the impact of indirect exposure to terrorism through media-television and Facebook-on adolescents' mental health in relation to terrorist attack on APSP. Moreover, the effect of changes occurred in parents' behaviour and school settings after the stated terrorist attack were also considered. To gather data, six Focus Group Discussions (FGDs) were conducted with adolescents (N = 45) who were enrolled in educational institutes far from the epicentre of the attack and came to know about the attack through media. Acquired qualitative data was subjected to thematic analysis revealing that indirect exposure to terrorism through media negatively impact the mental health of adolescents. Likewise, the abrupt changes in school settings and parental behaviour cause disruption in their immediate environment that further impacts their well-being.
  10. Luo Z, Zhang DW
    Front Psychol, 2025;16:1476928.
    PMID: 40099022 DOI: 10.3389/fpsyg.2025.1476928
    Attention-deficit/hyperactivity disorder (ADHD) is a prevalent neurodevelopmental disorder characterized by multiple neurocognitive deficits. Research suggests that music interventions, both active and passive, may be an effective complementary method of addressing ADHD challenges. This narrative review discusses seven potential neurocognitive mechanisms through which music interventions may help mitigate or alleviate ADHD symptoms, including executive function enhancement, timing improvement, arousal regulation, default mode network modulation, neural entrainment, affective management, and social bonding facilitation. Our study synthesized evidence from ADHD-specific studies and examined parallels to other populations to identify possible pathways through which music therapy could exert its effect. The paper also discusses the implications of individualized music interventions tailored to specific neurocognitive profiles in ADHD, advocating additional research to refine and optimize these approaches. Overall, music therapy has substantial potential as a complementary treatment for ADHD, offering new avenues for addressing the psychosocial and cognitive aspects of this condition.
  11. Zuo R, Guo X, Song X, Gao X, Zhang J, Jiang S, et al.
    J Pharm Anal, 2025 Mar;15(3):101080.
    PMID: 40099206 DOI: 10.1016/j.jpha.2024.101080
    The small-molecule alkaloid halofuginone (HF) is obtained from febrifugine. Recent studies on HF have aroused widespread attention owing to its universal range of noteworthy biological activities and therapeutic functions, which range from parasite infections and fibrosis to autoimmune diseases. In particular, HF is believed to play an excellent anticancer role by suppressing the proliferation, adhesion, metastasis, and invasion of cancers. This review supports the goal of demonstrating various anticancer effects and molecular mechanisms of HF. In the studies covered in this review, the anticancer molecular mechanisms of HF mainly included transforming growth factor-β (TGF-β)/Smad-3/nuclear factor erythroid 2-related factor 2 (Nrf2), serine/threonine kinase proteins (Akt)/mechanistic target of rapamycin complex 1(mTORC1)/wingless/integrated (Wnt)/β-catenin, the exosomal microRNA-31 (miR-31)/histone deacetylase 2 (HDAC2) signaling pathway, and the interaction of the extracellular matrix (ECM) and immune cells. Notably, HF, as a novel type of adenosine triphosphate (ATP)-dependent inhibitor that is often combined with prolyl transfer RNA synthetase (ProRS) and amino acid starvation therapy (AAS) to suppress the formation of ribosome, further exerts a significant effect on the tumor microenvironment (TME). Additionally, the combination of HF with other drugs or therapies obtained universal attention. Our results showed that HF has significant potential for clinical cancer treatment.
  12. Wong A, Cashmore R, Roslan NL, Ahmed N, Ibrahim M, Kuhn I, et al.
    J Intensive Care Soc, 2025 Mar 15.
    PMID: 40099279 DOI: 10.1177/17511437251326744
    BACKGROUND: POCUS is increasingly in utility and application across a variety of specialities. Although several professional societies, including the European Society of Intensive Care Medicine (ESICM) have recommended focussed Neurological ultrasound be a core competency, recommendations on how best to achieve this competency are lacking.

    AIM: The purpose of this systematic review was to assess what best-practice and evidence-based recommendations are available to achieve competency in Neurological Point-of-care ultrasound (NeuroPOCUS).

    METHODS: We undertook a structured systematic review of publications and studies on the training of NeuroPOCUS for intensive care.

    RESULTS: Our search strategy yielded a total of 4965 publications, reduced to 3551 following de-duplication. Despite this, only 28 publications were relevant following review of title and abstract and only 12 of these on full-text review. We identified three prospective studies, four consensus statements and five publications as posters and published abstracts. Structured analysis of these revealed minimal evidence-based teaching recommendations, and significant variability in teaching method.

    CONCLUSION: Compared to other modalities of POCUS (e.g. Cardiac and Lung), NeuroPOCUS lags behind with regards to utilisation and training in the general intensive care setting. Further work is needed to establish how this technique can be effectively taught and assessed for clinical application.

  13. Chang WJ, Liew SY, Kurz T, Tan SP
    Beilstein J Org Chem, 2025;21:596-600.
    PMID: 40099301 DOI: 10.3762/bjoc.21.46
    The hydantoin scaffold is renowned for its wide-ranging biological activities, including antibacterial, antiviral, anticancer, anti-inflammatory, and anticonvulsant effects. In this study, we present an innovative, sustainable approach to synthesizing hydantoins (H2a-j) directly from amino acids. This method employs a column chromatography-free, two-step, one-pot microwave-assisted synthesis that delivers hydantoins in yields ranging from 34% to 89%. The protocol demonstrates exceptional functional group tolerance, accommodating phenyl, aliphatic, phenol, alcohol, heterocyclic, and sulfide groups. This scalable, rapid, and eco-friendly strategy offers a promising avenue for the efficient synthesis of hydantoins, aligning with green chemistry principles and expanding the accessibility of these bioactive compounds for pharmaceutical applications.
  14. Pawar A, Dere S, Pandhare R, Mohite P, Alharbi HM, Subramaniyan V, et al.
    Sci Rep, 2025 Mar 14;15(1):8900.
    PMID: 40087313 DOI: 10.1038/s41598-025-90962-9
    The main objective of the present study was to improve the solubility and dissolution rate of felodipine (FLD), a drug that does not dissolve well in water, using a self-nanoemulsifying drug delivery system (SNEDDS). Many analyses have been performed in the laboratory using different oils, non-ionic surfactants, and water-soluble co-solvents to prepare FLD-loaded SNEDDS. It involves measurements of viscosity, refractive index, and droplet size. Solubility studies revealed the best way to load drugs, and pseudo-ternary phase diagrams showed the right amounts of surfactant and co-surfactant for preparing the nanoemulsion. An in vitro dissolution study showed that SNEDDS worked better than pure FLD, releasing over 95% of FLD within 20 min. SNEDDS loaded with felodipine are a good option for developing new oral medicines because they can hold more drugs, dissolve better, and dissolve more quickly. This new SNEDDS technology shows promise for improving the performance of drugs that do not dissolve well, which could lead to better therapeutic results.
    MeSH terms: Solubility; Emulsifying Agents
  15. Nizar MABM, Seo B, Hussaini HM, Young B, Rich AM
    Int J Paediatr Dent, 2025 Mar 14.
    PMID: 40087399 DOI: 10.1111/ipd.13309
    BACKGROUND: Alveolar lymphangioma is a rare condition that commonly develops on the alveolar ridge of Black male neonates. It typically presents as a bluish, dome-shaped swelling smaller than 1 cm on the posterior alveolar ridge of the mandible or/and maxilla, that allows it to be diagnosed clinically. Most previously reported cases of alveolar lymphangioma report spontaneous resolution, and biopsy is seldom performed unless the lesion becomes symptomatic or interferes with feeding.

    CASE REPORT: We report a case of oral alveolar lymphangioma on the anterior maxillary alveolar ridge of an 11-day-old infant of non-black descent. The lesion presented as a pedunculated, reddish, lobular mass in the anterior maxilla. Given its atypical presentation and the potential for feeding difficulties, an excisional biopsy was performed. The diagnosis was confirmed histologically through biopsy and supported by immunohistochemical staining.

    CONCLUSION: This case expands the understanding of alveolar lymphangioma by reporting it in a non-Black neonate with an unusual location and appearance, emphasising the need for excisional biopsy to rule out other potential oral lesions, especially neoplasms.

  16. Godela R, Nelson VK, Nuli MV, Jaini PK, Pathak S, Ponnekanti K, et al.
    BMC Pharmacol Toxicol, 2025 Mar 14;26(1):60.
    PMID: 40087706 DOI: 10.1186/s40360-025-00892-5
    The current work aims to develop a reliable and robust RP-HPLC method for analyzing Fosamprenavir and its potential impurities, including isomer, amino, propyl, nitro, and Amprenavir. The method used a Zobrax C18 column with a mobile phase of 0.1% V/V orthophosphoric acid in water and acetonitrile in gradient elution at a flow rate of 1 mL/min to accomplish efficient separation with detection at 264 nm and column temperature of 30 ± 20C. A diluent with a 1:1 water-to-acetonitrile ratio was used to prepare standard and sample solutions. The developed approach was validated as per ICH Q2(R1) guidelines. Fosamprenavir, Amino, Propyl, Isomer, Nitro impurities, and Amprenavir impurities were eluted at retention time (RT) of 5.3 min, 2.3 min, 4.3 min, 4.7 min, 8.1 min and 8.6 min correspondingly with good resolution within a 10-minute run time. Method validation confirmed system suitability, linearity (R² = 0.999), good sensitivity (LOD/LOQ), specificity, precision (% RSD: 0.5-1.7), accuracy (% recovery: 90.9-104.3%), and robustness. The optimized approach excelled existing methods in lower retention time, run time, sensitivity, and linearity for all potential impurities, making it a novel and trustworthy method for monitoring Fosamprenavir drug quality and assessing stated impurities. The established method allows precise measurement of Fosamprenavir and related substances, supporting drug safety and regulatory compliance.
    MeSH terms: Chromatography, High Pressure Liquid/methods; Morpholines/analysis; Morpholines/chemistry; Organophosphates/analysis; Reproducibility of Results; Chromatography, Reverse-Phase/methods; Chromatography, Reverse-Phase/standards; Limit of Detection
  17. Chia WX, Chung FF, Chia YC, Ng JY, Lee KH, Gunawan K, et al.
    BMC Nutr, 2025 Mar 14;11(1):54.
    PMID: 40087710 DOI: 10.1186/s40795-025-01037-x
    BACKGROUND: Prioritizing policies that address major non-communicable disease (NCD) risk factors, such as poor diet, is a cost-effective way to improve health outcomes. While education can enhance knowledge and practices in the short term, recent trends show that structural and policy-based initiatives, such as setting mandatory salt, sugar, and oil (SOS) targets are effective means of changing dietary behaviours and reducing NCD risk factors. However, few studies have surveyed the KAP of food vendors in workplaces and educational institutions and assessed their receptiveness to serving low-SOS dishes. This study aimed to evaluate the knowledge, attitudes, and practices (KAP) regarding salt, oil, and sugar (SOS) consumption and use, as well as the barriers, and enablers of these practices amongst food operators within Malaysia's out-of-home sector.

    METHODS: This study employed a cross-sectional, descriptive survey design to assess knowledge, attitudes, and practices (KAP) related to the consumption of salt, oil, and sugar and their use in food preparation amongst campus canteen staff at two Malaysian higher education institutions. Data were collected anonymously from 38 participants, which included representatives from all independent canteen operators on the premises. The study also explored the barriers and enablers vendors encounter in reducing salt and sugar usage.

    RESULTS: The study found that most participants were aware of the health risks associated with high salt, oil, and sugar (SOS) consumption and had positive attitudes towards reducing dietary SOS intake in general, though these attitudes did not extend to their own food preparations. However, their knowledge of recommended dietary guidelines was poor, and actual practices related to SOS reduction were inadequate. Barriers to reducing SOS included concerns about taste and customer acceptance, as well as the lack of standardized guidelines and tools for measuring SOS in food preparation.

    CONCLUSIONS: This study identifies gaps in knowledge and practices related to SOS reduction among the surveyed food vendors. While vendors acknowledge the health risks of high SOS consumption, there is a lack of awareness of dietary guidelines and practical low-SOS cooking methods, highlighting the need for regular monitoring, vendor education, and support in promoting healthier food options.

  18. Yang Y, Razak SRA, Ismail IS, Ma Y, Yunus MA
    Cancer Cell Int, 2025 Mar 14;25(1):94.
    PMID: 40087755 DOI: 10.1186/s12935-025-03666-5
    Cancer remains a major global health challenge due to its rising prevalence and high mortality rates. The field of microRNAs (miRNAs) has made significant progress in the understanding of tumorigenesis and has broadened our knowledge of their targeting, especially in cancer therapy. miRNAs, a class of small non-coding RNAs, participate in post-transcriptional gene regulation by translational inhibition or mRNA degradation. Among these, microRNA-192 (miR-192) is located on human chromosome 11q13.1, and is highly correlated with the occurrence and development of various human cancers. Dysregulation of miR-192 has been extensively studied in various pathological processes, including tumorigenesis, making it a valuable biomarker for cancer diagnosis and prognosis. The functional role of miR-192 varies across cancer types, acting as either a tumor suppressor or as an oncogene through the modulation of multiple gene expressions and downstream signaling pathways. However, the roles of miR-192 in cancer appear inconsistent across types, with current research often focused on specific genes or pathways, limiting insight into its broader impact on cellular signaling networks. Therefore, this review aims to provide a comprehensive overview of miR-192 research. The paper reviews differences in miR-192 expression in cancer and systematically summarizes the role of miR-192 in cancers. The review further explores the complex roles of miR-192 in various pathological processes, emphasizing its regulatory pathways, interaction networks, and association with tumor progression. This review also illustrates the clinical application of miR-192 as a diagnostic and prognostic biomarker for non-invasive cancer detection, as it is consistently present in both serum and exosomes. A comprehensive summary and analysis of the relationship between miR-192 and various cancers may provide valuable insights, potentially guiding novel approaches in clinical diagnosis, therapeutic strategies, and foundational cancer research.
  19. Abbas L, Muhamad Nor I, Ismail F, Palaniappan S, Chong GY, Wan Puteh SE, et al.
    Thromb J, 2025 Mar 14;23(1):25.
    PMID: 40087785 DOI: 10.1186/s12959-025-00710-2
    BACKGROUND: Patients with solid organ cancers are at increased risk of developing cancer-associated thrombosis (CAT), a complication driven by a complex interplay of patient-specific factors, cancer characteristics, and treatment modalities. Data on CAT and its associated risk factors within diverse ethnic groups, such as the Malaysian population, remains limited. This observational, cohort study aimed to address this gap by determining the incidence of CAT and identifying associated risk factors among multi-ethnic Malaysian patients with solid organ cancers.

    METHODS: This study included solid organ cancer patients aged ≥ 18 who attended HCTM and HKL from May 2022 to August 2023. The baseline demographics, and clinical characteristics, were acquired at the cancer diagnosis. Data on cancer treatment, thrombotic events and anticoagulation therapy during the study and its treatment were documented. Multivariable logistic regression analysis was performed to determine the independent factors associated with CAT in solid organ cancer.

    RESULTS: A total of 250 solid organ cancer patients were included, with a mean age of 57.7 (13.7) years. This multi-ethnic cohort consisted of mostly Malay patients (55.2%), followed by Chinese (33.2%) and Indian & others (11.6%). The prevalence of CAT at baseline was 4.8%, while the incidence of CAT during follow-up was 12%. Poor performance status and obesity were associated with CAT at baseline. Univariable logistic regression showed platelets ≥ 450 × 109/L and Khorana score ≥ 3 had significantly higher risks of CAT at baseline. Stage IV disease, radiotherapy and chemotherapy, namely platinum-based chemotherapy and antimetabolites were associated with CAT during follow-up. The ROC analysis showed that the KRS significantly predicted CAT (area under the curve, 0.701 (95%CI: 0.594-0.808, p = 0.001).

    CONCLUSIONS: This study highlights the prevalence of CAT at baseline and the incidence of CAT during follow-up, similar to other studies. Patients' clinical characteristics were associated with CAT at baseline while disease and treatment factors were associated with CAT at follow-up. These findings emphasise the need for targeted thromboprophylaxis in high-risk populations and highlight the importance of risk stratification tools such as the Khorana score for optimal patient management. Future studies involving larger patient cohorts and longer study duration would be beneficial. These findings provide valuable insights to inform clinical decision-making, optimise patient outcomes, and minimise potential risks.

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