Browse publications by year: 2025

  1. Sasmito SD, Taillardat P, Adinugroho WC, Krisnawati H, Novita N, Fatoyinbo L, et al.
    Nat Commun, 2025 Jan 28;16(1):740.
    PMID: 39875368 DOI: 10.1038/s41467-025-55892-0
    Southeast Asia (SEA) contributes approximately one-third of global land-use change carbon emissions, a substantial yet highly uncertain part of which is from anthropogenically-modified peat swamp forests (PSFs) and mangroves. Here, we report that between 2001-2022 land-use change impacting PSFs and mangroves in SEA generate approximately 691.8±97.2 teragrams of CO2 equivalent emissions annually (TgCO2eyr-1) or 48% of region's land-use change emissions, and carbon removal through secondary regrowth of -16.3 ± 2.0 TgCO2eyr-1. Indonesia (73%), Malaysia (14%), Myanmar (7%), and Vietnam (2%) combined accounted for over 90% of regional emissions from these sources. Consequently, great potential exists for emissions reduction through PSFs and mangroves conservation. Moreover, restoring degraded PSFs and mangroves could provide an additional annual mitigation potential of 94.4 ± 7.4 TgCO2eyr-1. Although peatlands and mangroves occupy only 5.4% of SEA land area, restoring and protecting these carbon-dense ecosystems can contribute substantially to climate change mitigation, while maintaining valuable ecosystem services, livelihoods and biodiversity.
    MeSH terms: Asia, Southeastern; Carbon Dioxide/analysis; Malaysia; Ecosystem; Wetlands*; Forests*
  2. Zaman R, Tan ESS, Bustami NA, Amini F, Seghayat MS, Ho YB, et al.
    Sci Rep, 2025 Jan 28;15(1):3507.
    PMID: 39875543 DOI: 10.1038/s41598-025-87680-7
    Opuntia ficus-indica (OFi) is a major fruit source prevalent in semiarid and arid regions across various countries worldwide. It is widely recognised for its potential health benefits; however, most studies investigating its effects have been limited to pre-clinical models, highlighting the need for further validation through clinical trials. This study aimed to evaluate the effectiveness of OFi supplementation in enhancing antioxidant levels. Fifty healthy participants, aged 18 years and older, including males and females, received a daily OFi supplement of 1500 mg for 3 months. These findings revealed a significant 48.1% increase in salivary total antioxidant capacity (TAC) (P 
    MeSH terms: Adolescent; Adult; Female; Humans; Male; Malondialdehyde/analysis; Malondialdehyde/metabolism; Middle Aged; Plant Extracts/administration & dosage; Saliva/metabolism; Saliva/chemistry; Biomarkers/analysis; Dietary Supplements*; Young Adult
  3. Hailu H, Incollingo Rodriguez AC, Rodriguez A, Skouteris H, Hill B
    Int J Obes (Lond), 2025 Jan 28.
    PMID: 39875594 DOI: 10.1038/s41366-025-01725-5
    BACKGROUND: Weight bias is a global health challenge and community members are endorsed as the most common source of weight bias. The nature of weight biases specifically against preconception, pregnant, and postpartum (PPP) women from the perspective of community members is not known, especially in terms of cross-cultural trends. We investigated the magnitude of explicit and implicit weight bias and profiles of characteristics associated with harbouring weight bias.

    METHODS: We conducted a multinational investigation of clusters of factors associated with weight bias against PPP women (May-July 2023). Community members from Australia, Canada, United States (US), United Kingdom (UK), Malaysia, and India completed a cross-sectional survey measuring explicit and implicit weight biases, beliefs about weight controllability, and awareness of sociocultural body ideals. Hierarchical multiple regression and latent profile analyses identified clusters of factors associated with weight bias.

    RESULTS: Participants from India reported the lowest explicit weight bias (B = -0.45, p = 0.02). Participants from Australia (B = -0.14, p = 0.04) and the UK (B = -0.16, p = 0.02) (vs. US) reported the lowest implicit weight bias. Three distinct profiles were identified clustering on body mass index (BMI) and weight-controllability beliefs: low-BMI/moderate-beliefs, high-BMI/more biased beliefs, and high-BMI/less biased beliefs. Profile membership varied by country of residence and weight bias outcomes with low-BMI/moderate-beliefs profiles containing more people from non-Western countries and with low explicit weight bias.

    CONCLUSIONS: Explicit and implicit weight bias was harboured by participants across all included nations, although less pronounced in non-Western countries. Our profiles highlight that individuals who held a stronger belief that weight is controllable, regardless of their body weight, should be targeted for interventions to eliminate weight stigma.

  4. Shyam S, Tan SY, ALsheibani BAA, How KE, Morpeth A, Rippin H, et al.
    PMID: 39876534 DOI: 10.1177/10105395241313419
    Nutritional quality of food for infants and young children (FIYC) is key to optimum development and health. To evaluate commercial FIYC products, the World Health Organization Regional Office for Europe (WHO-EURO) has defined a Nutrient and Promotion Profile Model (NPPM). Although FIYC is commonly used in Malaysia, its quality is largely unknown. We evaluated the nutrient profiles and on-pack promotions of FIYC available in Kuala Lumpur, Malaysia, using the NPPM. Among the 285 products sampled from a range of retail outlets, none met all NPPM promotional recommendations; only 24% satisfied all relevant nutrition criteria. Several products failed on sugar (29%) and sodium (26%) criteria, with around half having added sugar. In addition, 3%, 6%, and 9% of FIYC failed relevant fat, protein, and energy requirements, respectively. Although 98% had inappropriate health claims, 36% missed appropriate breastfeeding statements. There is an urgent need to improve the quality of FIYC in Malaysia.
  5. Kamal F, Othman HR
    Am J Case Rep, 2025 Jan 29;26:e945579.
    PMID: 39876542 DOI: 10.12659/AJCR.945579
    BACKGROUND Lupus anticoagulants (LA) can interfere with routine coagulation tests such as the activated partial thromboplastin time (aPTT) and prothrombin time (PT). The international normalized ratio (INR) is derived from PT and is used to monitor warfarin therapy. A positive LA result is one of the laboratory criteria of the 2023 ACR/EULAR antiphospholipid syndrome (APS) classification criteria. We report a case in which LA interfered with INR measurement in an APS patient. CASE REPORT Our patient was a 45-year-old man who had experienced multiple episodes of thromboembolism. His INR was consistently high, despite not being on any anticoagulant. Our laboratory used a recombinant PT reagent, Siemens Healthineers Dade® Innovin® on a fully automated coagulometer, the Sysmex CS-2500. PT measurements were repeated using 2 different analyzers, the Sysmex CA-104 and Werfen ACL Top 550 CTS. The PT results were 40.5 s (reference range (RR): 9.3-10.8 s) and 56 s, using Sysmex CS2500 and CS104, respectively. However, the PT was 13.4 s (RI: 10.3-12.7 s) using Werfen ACL Top 550 CTS. We retested the sample using Thromborel® S, a tissue-derived PT reagent, and PT was found to be within the reference range. The patient tested positive for LA, anti-cardiolipin, and anti-beta2 glycoprotein I antibodies. CONCLUSIONS LA can falsely prolong the PT when a recombinant PT reagent is used. When we retested the plasma using a tissue-derived PT reagent - Thromborel® S - PT was within normal limits. Thus, it is important to acknowledge that LA can react differently with different PT reagents.
    MeSH terms: Humans; Male; Middle Aged; Prothrombin Time*; Thromboembolism; International Normalized Ratio*
  6. Koh HP, Ab Jalal H, Koo YP, Chen MSY, Selvaratanam M, Bhuvanendran Pillai A, et al.
    J Pharm Policy Pract, 2025;18(1):2457410.
    PMID: 39877033 DOI: 10.1080/20523211.2025.2457410
    BACKGROUND: The emergency medicine (EM) pharmacist is an integrated part of the Emergency Department (ED) interdisciplinary team in many countries, including Malaysia. The presence of EM pharmacists in the ED has positively impacted patient outcomes. Data on EM pharmacists' interventions is scarce in the Asian region. In Malaysia, data on interventions done by EM pharmacists in the EDs was unavailable. This study aimed to assess the type of interventions done by EM pharmacists in the ED of tertiary public hospitals in Malaysia.

    METHODS: This cross-sectional, multicenter study involved EM pharmacists from 14 tertiary hospitals in Malaysia. All accepted interventions done by EM pharmacists in the ED for patients admitted to the Red (critical) and Yellow (semi-critical) zones from January to June 2022 were extracted from the Clinical Pharmacy Report Form. All data were analyzed descriptively.

    RESULTS: The EM pharmacists documented 1659 accepted interventions on 1584 patients during the study period. Inappropriate regimens (n = 1117, 67.3%) and incomplete prescriptions (n = 339, 20.4%) were the main categories of accepted interventions in ED. Inappropriate drug (n = 574, 34.6%), dose (n = 292, 17.6%), and frequency (n = 176, 10.6%) were the top three subcategory interventions documented under inappropriate regimens. Antimicrobials, antihypertensives, and proton pump inhibitors were the commonest drug intervened under the categories of inappropriate drug intervention. There were 272 (16.4%) accepted interventions on high-alert medications (HAMs). Insulin, enoxaparin, and noradrenaline were the most intervened HAMs.

    CONCLUSION: Inappropriate treatment regimens were the most common intervention category done by EM pharmacists in Malaysia. The significant number of interventions done by EM pharmacists demonstrated the importance of EM pharmacists as integral members of the EM team. This data can help improve the quality of clinical pharmacy services in the ED and is important for the future expansion of clinical pharmacy services in all EDs across Malaysia, neighbouring countries, and other developing countries.

  7. Li XL, Dai YC, Liu ZB, Jiang YH, Liu HG, Yuan Y
    MycoKeys, 2025;112:211-232.
    PMID: 39877126 DOI: 10.3897/mycokeys.112.127011
    Nigroporusvinosus (Berk.) Murrill, first described from North America, was considered to be a common species in China. The existence of a species complex is confirmed through a phylogenetic analysis of samples examined. Based on morphological examination and molecular evidence, four new species are described as Nigroporusaustralianus, N.austroasianus, N.subvinosus and N.yunnanensis. They are characterized by pileate, effused-reflexed to resupinate, purplish, vinaceous to brown basidiomata when fresh, mostly becoming brown when dry. Nigroporusaustralianus is characterized by narrower basidiospores measuring 3.4-4.1 × 1.3-1.5 µm, thicker contextual hyphae measuring 3.2-6.4 µm in diam and a geographical distribution in Australia. Nigroporusaustroasianus is characterized by smaller pores measuring 10-13 per mm, generative hyphae dominant in the tube trama, small basidiospores measuring 3-4.1 × 1.5-2 μm and a distribution in Malaysia and tropical to subtropical regions of China. Nigroporussubvinosus is characterized by skeletal hyphae with thin to slightly thick walls, barrel- to pear-shaped basidia, and long cystidioles measuring 10-18 µm and is common in Asia. Nigroporusyunnanensis is characterized by thinner pilei measuring 2.5 mm thick at the base, bigger basidiospores measuring 4-4.5 × 1.9-2.2 μm and is found only in Yunnan. The 2-gene (ITS+nLSU) analysis of the Steccherinaceae indicated that the four new species nested in the Nigroporus clade. The 3-gene (ITS+nLSU+TEF1) analysis of the genus Nigroporus showed that N.australianus formed a monophyletic lineage, N.subvinosus was sister to N.austroasianus and N.yunnanensis, and N.austroasianus was sister to N.yunnanensis. Furthermore, N.vinosus sensu stricto is also distributed in Asia.
  8. Hiew SY, Low BY
    Explor Res Clin Soc Pharm, 2025 Mar;17:100556.
    PMID: 39877296 DOI: 10.1016/j.rcsop.2024.100556
    BACKGROUND: Active pharmaceutical residues introduced into the environment through irresponsible household disposal of unused and expired medication can damage nature and health. Through medication take-back programmes, these risks can be mitigated. However, healthcare professionals' neglect of their responsibility to ensure proper disposal practices has perpetuated subpar norms among the public.

    OBJECTIVES: The objectives of this systematic review were to assess the knowledge, attitude, and practice of household pharmaceutical waste disposal among healthcare professionals and healthcare professional students as well as to compare their respective levels of knowledge, attitude and practice.

    METHODS: A systematic search of published articles from 2014 to 2023 in three online journal databases (Pubmed, Scopus, and Web of Science) yielded an initial pool of 10,381 records, which was narrowed down by title and abstract screening to 46 relevant publications for full-text examination and the final inclusion of 21 papers for data extraction and synthesis.

    RESULTS: The findings revealed deficiencies in academic curricula and medication disposal training, leading to inadequate knowledge and perceptions among healthcare professionals. Many healthcare professionals consider public education about safe medication disposal practices to be outside their job descriptions, leading to infrequent communication of medication disposal methods to their consumers and service users. Additionally, inadequate and inaccessible infrastructure further hinders proper medication disposal practices despite awareness of the consequences.

    CONCLUSIONS: This study provides insights for policymakers and educators to address these and enhance healthcare professionals' participation in improving safe medication disposal practices. Efforts to strengthen training programmes, incorporate comprehensive education on medication disposal into curricula, and improve infrastructure for safe medication disposal are essential to effectively address the issue of household pharmaceutical waste disposal.

  9. Abdillah RAK, Budhy TI, Narmada IB, Ardani IGAW, Ramadhani NF, Sitalaksmi RM, et al.
    J Oral Biol Craniofac Res, 2025;15(1):170-177.
    PMID: 39877333 DOI: 10.1016/j.jobcr.2024.12.017
    OBJECTIVE: The orthodontic mini-implant (OMI) failure often occurs due to the accumulation of peri-implantitis bacteria surrounding it, which results in a stable, resistant form of absolute skeletal anchorage during orthodontic treatment. Administering doxycycline may be the solution, but long-term side effects result in antibiotic resistance. Roselle flowers (Hibiscus sabdariffa) possess beneficial active phytochemical substances, which may have potential as an OMI peri-implantitis alternative therapy. This study investigates the antibacterial activity of 1 % Roselle flower (H. sabdarifa) nanoemulsion (NE) extract (1 % RNE) toward peri-implantitis bacteria in OMIs.

    METHODS: A phytochemical analysis of 1 % RNE was carried out to examine the active substances possessed in it, such as flavonoids, quinone, saponin, alkaloids, tannins, terpenoids, and steroids. Nanoemulsion characterization was carried out using a particle size analyzer (PSA). The antibacterial activity of 1 % RNE toward Prevotella intermedia (Pi), Porphyromonas gingivalis (Pg), Aggregatibacter actinomycetemcomitans (Aa), and Fusobacterium nucleatum (Fn) was carried out to determine the minimum inhibitory concentration, minimum bactericidal concentration, and inhibitory zone compared with doxycycline as a positive control.

    RESULTS: Roselle flower NE extract (1 %) possessed flavonoids, quinone, saponin, alkaloids, tannins, terpenoids, and steroids positively. The PSA showed that the 1 % RNE had a size of 98.13 d nm. The antibacterial activity of 1 % RNE against Aa, Pg, Pi, and Fn bacteria at 3.125 % showed significant differences (p 

  10. Mudalige T, Pathiraja V, Delanerolle G, Cavalini H, Wu S, Taylor J, et al.
    BJUI Compass, 2025 Jan;6(1):e464.
    PMID: 39877583 DOI: 10.1002/bco2.464
    BACKGROUND: Pelvic organ prolapse (POP) occurs when one or more pelvic organs (uterus, bowel, bladder or top of the vagina) descend from their normal position and bulge into the vagina. Symptoms include pelvic discomfort, fullness, and changes in bladder or bowel function. Treatment ranges from conservative approaches to surgery, depending on symptom severity. Surgical methods include vaginal wall repair, with or without hysterectomy, or via laparoscopic, robotic or open techniques. Common complications include bleeding, infection, and urinary or bowel dysfunction.

    METHODS: A systematic review was conducted, and a protocol was registered with PROSPERO (CRD42022346051). Publications from 30 April 1980 to 30 April 2023 were retrieved from multiple databases. Data were analysed using random-effects and common-effects models with subgroup and sensitivity analyses.

    FINDINGS: Forty-four studies met the inclusion criteria, with 29 studies used for meta-analysis of vaginal prolapse surgery outcomes. Sixteen studies focused on patients who had undergone hysterectomy alongside prolapse repair.

    INTERPRETATION: Patients who underwent vaginal prolapse surgery with hysterectomy experienced higher operative and postoperative complication rates than those without hysterectomy. Increased risks included hospital readmission, POP recurrence and re-operation. The review highlighted a lack of diversity in terms of ethnicity, age and comorbidity status, which are essential to fully understanding the impact of POP. Future research should focus on these underrepresented factors.

  11. Sia TL, Lai CD, Manan K, Khiu FL, Bakhtiar SZ, Chor YK, et al.
    IDCases, 2025;39:e02149.
    PMID: 39877723 DOI: 10.1016/j.idcr.2025.e02149
    We report a first case of ceftazidime-resistant pediatric melioidosis involving a previously healthy seven-year-old boy who presented with right lobar pneumonia complicated with a 5-cm lung abscess. Ceftazidime was initiated on Day-6 of admission when Burkholderia pseudomallei (ceftazidime-susceptible, minimum inhibitory concentration [MIC] 1.0 mcg/mL) was isolated from blood. Despite ceftazidime therapy at the recommended dosage, he developed fulminant septic shock and respiratory failure on Day-18 of hospitalization, requiring invasive ventilation, hemodynamic support, and continuous renal replacement therapy. His antibiotic was empirically escalated to meropenem; ceftazidime-resistant B. pseudomallei (MIC 32 mcg/mL) was subsequently isolated from blood and endotracheal secretions. He improved after two weeks of intensive care and was discharged well after two months of hospitalization. Our literature review on ceftazidime-resistant B. pseudomallei infection indicates that acquired resistance is a rare but potentially lethal treatment-related complication. All melioidosis patients should be carefully monitored during treatment with ceftazidime (or other β-lactams) for the development of antimicrobial resistance.
  12. Albasri A, Gouda W, Alsaqabi F, Magdy MM, Mostafa AA, El Ghobashy N
    J Int Med Res, 2025 Jan;53(1):3000605241311446.
    PMID: 39877975 DOI: 10.1177/03000605241311446
    Behçet's disease (BD) is a rare systemic vasculitis that is characterized by recurrent oral and genital ulcers, uveitis, and skin lesions. Although neurological involvement is a known complication, ischemic stroke remains uncommon. Herein, we report a 37-year-old Kuwaiti woman who experienced recurrent ischemic stroke with no traditional risk factors. Imaging studies, including magnetic resonance imaging and angiography, confirmed the diagnosis of recurrent ischemic stroke and identified the underlying vasculitic changes. A detailed examination revealed a history of recurrent orogenital ulcers, leading to a diagnosis of BD. High-dose corticosteroids, azathioprine, and antiplatelet treatment were effectively used to manage her acute strokes and underlying vasculitis, thus preventing further thrombotic events. Our case report underscores the difficulties of diagnosing BD with ischemic stroke as the initial manifestation and emphasizes the importance of a multidisciplinary approach that involves rheumatologists, neurologists, and radiologists to create an effective treatment plan. Our findings also underscore the need for medical practitioners to be more knowledgeable about ischemic stroke in young patients without risk factors from endemic areas of BD, and to develop effective therapy plans. Our review of the English literature indicates that this is only the fifth report of stroke as the first presentation of BD.
    MeSH terms: Adrenal Cortex Hormones/therapeutic use; Adult; Azathioprine/therapeutic use; Female; Humans; Magnetic Resonance Imaging; Recurrence*
  13. Farooq S, Ngaini Z
    Curr Top Med Chem, 2025;25(1):47-62.
    PMID: 38847246 DOI: 10.2174/0115680266303704240524080333
    Flavonoids belong to the polyphenol group that naturally exists in fruits, vegetables, tea, and grains. Flavonoids, as secondary metabolites, show indispensable contributions to biological processes and the responses of plants to numerous environmental factors. The bioactivity of flavonoids depends on C6-C3-C6 ring substitution patterns that exhibit bioactive antioxidant, antimicrobial, antifungal, antitumor, and anti-inflammatory properties. The synthesis of flavonoids has been reported by various methodologies. Therefore, the present review systematically summarizes the synthesis of recent heterocyclic flavonoid derivatives via facile synthetic approaches since the research in flavonoids is useful for therapeutic and biotechnology fields.
    MeSH terms: Anti-Infective Agents/chemical synthesis; Anti-Infective Agents/pharmacology; Anti-Infective Agents/chemistry; Anti-Inflammatory Agents/chemical synthesis; Anti-Inflammatory Agents/pharmacology; Anti-Inflammatory Agents/chemistry; Antineoplastic Agents/chemical synthesis; Antineoplastic Agents/pharmacology; Antineoplastic Agents/chemistry; Antioxidants/chemical synthesis; Antioxidants/pharmacology; Antioxidants/chemistry; Humans; Molecular Structure
  14. Krishnan S, Ademi Z, Malone D, Abebe TB, Lim A
    Simul Healthc, 2025 Feb 01;20(1):61-69.
    PMID: 39110425 DOI: 10.1097/SIH.0000000000000811
    INTRODUCTION: Online education games are gaining ground in health profession education, yet there is limited literature on its costs. This study is an economic evaluation of the substitution of a face-to-face (F2F) workshop with an online escape room game teaching the same content.

    METHODS: A traditional F2F workshop on hepatitis management was conducted with 364 students in 2021 and was compared with a virtual self-run escape room game called Hepatitiscape™, which was used by 417 students in 2022. The outcomes were final examination and objective structured clinical examination (OSCE) scores for hepatitis stations. An incremental cost-effectiveness ratio was used to compare costs and outcomes. Student perceptions of the delivery of Hepatitiscape™ were also captured using an online questionnaire.

    RESULTS: Delivering the hepatitis case workshop via Hepatitiscape™ yielded an additional 4.77% increase in the final examination score and a 21.04% increase in the OSCE score at an additional cost of AUD $4212 in the first year compared with F2F delivery. This equated to an incremental cost-effectiveness ratio of AUD 883 per additional score of final examination and AUD 200 per additional score of OSCE for hepatitis stations. Hepatitiscape™ became cost saving from the second year onward. Student perception data revealed their recall of content was higher owing to the iterative design of the gaming elements.

    CONCLUSIONS: Hepatitiscape™ is likely to be a cost-effective strategy to deliver workshops that are routinely delivered F2F to test knowledge-based constructs. In addition, virtual gaming has a logistical advantage over F2F delivery in that it enhances student participation from remote locations and allows for better control and flexibility of content delivery with increasing or decreasing cohort sizes, and can have potential long-term sustainable savings.

    MeSH terms: Clinical Competence; Cost-Benefit Analysis*; Educational Measurement; Hepatitis; Humans; Video Games; Education, Distance/methods; Education, Distance/organization & administration
  15. Washif JA, Pyne DB
    Int J Sports Physiol Perform, 2025 Feb 01;20(2):321-327.
    PMID: 39265975 DOI: 10.1123/ijspp.2024-0227
    BACKGROUND: In high-performance sport, the support provided by sport scientists and other staff can be a valuable resource for coaches and athletes.

    PURPOSE: We propose and detail here the approach of "minimal, adequate, and accurate" sport-science support to ensure that programs of work and solutions are both economical and effective.

    METHODS: Our support provision advocates for utilization of "minimal" resources (employing the least amount of time, tools, and funding) necessary to achieve the desired outcomes. We strive for "adequate" information that fulfills specific objectives without excess and with the requirement that methods and data used are "accurate" (valid and reliable). To illustrate the principles of this approach, we outline a real-world example of supporting 100-m track (athletics) sprinters preparing and competing in an international competition. The provision of performance support emphasizes an integrated approach, combining knowledge and insights from multiple sport-science disciplines. The key facets managed under this approach are (1) neuromuscular readiness, (2) wellness monitoring, (3) movement observation, (4) motivation, (5) biomechanics and performance analysis, and (6) qualitative feedback. These facets are based on the specific performance determinants and influencing factors of an event (100-m dash).

    CONCLUSIONS: Application of this quantitative and qualitative approach can enhance the ability to make informed decisions. Nevertheless, the approach must be planned, evaluated, and refined on a regular basis to enable effective decision making in sport-science support. The 3-element approach of "minimal, adequate, and accurate" should be codesigned and supported by the athletes, coaches, and staff to ensure successful implementation.

    MeSH terms: Biomechanical Phenomena; Competitive Behavior; Feedback; Humans; Motivation; Running/physiology
  16. Suschinel R, Jaimes-Mogollón AL, Sim SF, Ting W, Cáceres-Tarazona JM, Alvarez-Valdez E, et al.
    Anal Bioanal Chem, 2025 Feb;417(4):771-783.
    PMID: 39722103 DOI: 10.1007/s00216-024-05691-1
    The analysis of the volatile compounds released by biological samples represents a promising approach for the non-invasive diagnosis of a disease. The present study, focused on a population of dogs infected with canine leishmaniasis, aimed to decipher the volatolomic profile associated with this disease in dogs, which represent the main animal reservoir for Leishmania pathogen transmission to humans. The volatiles emitted by the breath and hair of dogs were analysed employing the gas chromatography-mass spectrometry (GC-MS) technique. The acquired chromatograms were investigated using a novel algorithm developed in this study for automated chromatographic peak detection and matching in untargeted GC-MS analysis, which includes various steps that comprise noise reduction, m/z filtering, background subtraction, peak detection, peak matching, and generation of a peak table for compounds identification. The results revealed one tentative breath volatile biomarker and five tentative hair volatile biomarkers for the cutaneous form of the disease, which is characterised by skin ulcerations. Additionally, nine tentative breath volatile biomarkers and four tentative hair volatile biomarkers were found for the visceral form of the disease, which affects internal organs such as spleen, liver and bone marrow. All tentative biomarkers identified in this study were upregulated in cutaneous leishmaniasis, while in visceral leishmaniasis, all tentative biomarkers were upregulated in the breath and only one out of four in the hair. Only one compound (glyceryl monooleate) was identified as tentative volatile biomarker for both forms of the disease, in the hair of dogs.
    MeSH terms: Algorithms*; Animals; Dogs; Leishmaniasis/diagnosis; Leishmaniasis/parasitology; Leishmaniasis/veterinary; Leishmaniasis, Visceral/diagnosis; Leishmaniasis, Visceral/veterinary
  17. Kam MYY
    J Sci Food Agric, 2025 Jan 27.
    PMID: 39868583 DOI: 10.1002/jsfa.14164
    In recent years, changes in dietary patterns from an omnivore diet to a moderate-to-restrictive diet that includes more plant food are becoming popular for various reasons and the associated health benefits. Despite the increased consumption of plant food as recommended by these seemingly healthy diets, micronutrient deficiency is still prevalent particularly among the health-conscious populations. The aim of this review is to help guide interventions by understanding micronutrient deficiency trends from a dietary habit and plant physiology context. In this review, the author discusses how modern agricultural practices coupled with climate change, and with particular emphasis on the extreme dietary habits that lack variation and excessive consumption, may contribute to an increased ingestion of antinutrients which in turn potentially exacerbate vitamin and mineral deficiencies. While plants possess a wide range of secondary metabolites that exert beneficial health effects, some of these compounds are also antinutrients that interfere with the digestion and absorption of nutrients and micronutrients. Furthermore, the article also raises questions concerning the fate of antinutrient traits in future crops that were to be redesigned with improved stress tolerance, and the impacts it may have on human nutrition and the environment. © 2025 Society of Chemical Industry.
  18. Daud MN
    J Phys Chem A, 2025 Feb 06;129(5):1478-1491.
    PMID: 39869460 DOI: 10.1021/acs.jpca.4c07219
    This study investigates the motion of an electron in a Coulomb potential driven by an intense linearly polarized XUV laser pulse analyzed using Gordon-Volkov wave functions. The wave function is decomposed into spherical partial waves to model the scattered electron wave packet after the recollision with a proton. This interaction triggers high harmonic generation, producing coherent X-ray pulses with frequencies that are integer multiples of the XUV field. The research presents a novel method for achieving atomic-scale resolution at nanometer and subfemtosecond levels, enabling observation of electron-proton collisions on an attosecond time scale. It emphasizes the coupling of fields that create resonances in the scattered electron through photon energy exchange with XUV and X-ray pulses, leading to the formation of a Rydberg electron with energy levels up to n = 27 and angular momentum components l = 13 and m = ± 1. The combination of XUV and high-frequency X-ray fields introduces new nonperturbative nonlinear phenomena characterized by differential cross sections derived using the Floquet-Lippmann-Schwinger equation in the first-order Born approximation. The analysis shows that backward-forward scattering involves XUV-electron energy exchange, with peak intensity along the laser polarization vector, while sideways scattering, dominated by X-ray-electron interaction, peaks perpendicular to the polarization. Additionally, the laser-assisted scattering process results in temporary electron capture in a dressed proton-bound state, followed by escape and ejection, with the free electron ponderomotive energy exceeding 10Up.
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