New method for the isolation of endothelial cells from large vessels

Ataollahi F 1 , Pingguan-Murphy B 2 , Moradi A 2 , Wan Abas WA 2 , Chua KH 3 , Abu Osman NA 2

Affiliations 

  • 1 Department of Biomedical Engineering, Faculty of Engineering, University of Malaya, Kuala Lumpur, Malaysia. Electronic address: Forough_at@yahoo.com
  • 2 Department of Biomedical Engineering, Faculty of Engineering, University of Malaya, Kuala Lumpur, Malaysia
  • 3 Biochemistry Department, Faculty of Medicine, University Kebangsaan Malaysia, Kuala Lumpur, Malaysia
Cytotherapy, 2014 Aug;16(8):1145-52.
PMID: 24831838 DOI: 10.1016/j.jcyt.2014.01.010

Abstract

Numerous protocols for the isolation of bovine aortic endothelial cells have been described in the previous literature. However, these protocols prevent researchers from obtaining the pure population of endothelial cells. Thus, this study aimed to develop a new and economical method for the isolation of pure endothelial cells by introducing a new strategy to the enzymatic digestion method proposed by previous researchers.

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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