Cervical cancer is preventable by detecting precancerous states through Pap smear screening, followed by appropriate treatment. In Malaysia, even with a high incidence of cervical cancer and accessible screening program, the uptake of Pap smear screening was still low. Lack of knowledge, embarrassment, fear for the test and results are the factors reducing participation. The aim of this study was to determine the change in knowledge and attitude regarding the Pap smear screening as well as knowledge on cervical cancer among women in Mukim ]aya Setia after educational intervention. A community trial was conducted in August 2005 where 80 women were randomly selected. The women were interviewed for assessment prior to the intervention, using a set of structured questionnaire. The women underwent educational intervention programmes on cervical cancer and Pap smear screening. After a week, the women were re-assessed using the same set of questionnaire for their post intervention knowledge and attitudes. Scores were given for each answer, which were totaled and analyzed. The mean (SD) age was 41.1 (10.37) years. Most were housewives (87.5%), within the low income group (91.2%), of less than RM 1000.00 income per household. Most of the women received their education up to the secondary level only (97.5%). Despite their poor economic status, most of them had more than three children (68.8%). There was a significant improvement in knowledge of Pap smear (mean difference= 4.3, 95% CI= 3.55, 5.10) and cervical cancer (mean difference= 10.7, 95% CI= 10.03, 11.37) as well as attitude to Pap smear (mean difference= 7.1, 95% CI= 5.72, 8.47) after educational intervention. The knowledge and attitudes towards Pap smear screening as well as knowledge on cervical cancer among rural women in Mukim ]aya Setia, Kota Bharu improved after educational intervention.