Affiliations 

  • 1 Malaysian Agricultural Research And Development Institute, Serdang 43400, Malaysia; Institute of Nano Electronic Engineering, Universiti Malaysia Perlis, Kangar 01000, Malaysia
  • 2 Institute of Nano Electronic Engineering, Universiti Malaysia Perlis, Kangar 01000, Malaysia; School of Microelectronic Engineering, Universiti Malaysia Perlis, Arau 02600, Malaysia
  • 3 Institute of Nano Electronic Engineering, Universiti Malaysia Perlis, Kangar 01000, Malaysia; Institute of Advanced Technology, Universiti Putra Malaysia, Serdang 43400, Malaysia
  • 4 Institute of Nano Electronic Engineering, Universiti Malaysia Perlis, Kangar 01000, Malaysia
  • 5 School of Bioprocess Engineering, Universiti Malaysia Perlis, Arau 02600, Malaysia
  • 6 Institute of Advanced Technology, Universiti Putra Malaysia, Serdang 43400, Malaysia
  • 7 Department of Electrical and Computer Engineering, Engineering Faculty, International Islamic University Malaysia, Kuala Lumpur 53100, Malaysia
Biosens Bioelectron, 2017 Jul 15;93:146-154.
PMID: 27660016 DOI: 10.1016/j.bios.2016.09.035

Abstract

Surface acoustic wave mediated transductions have been widely used in the sensors and actuators applications. In this study, a shear horizontal surface acoustic wave (SHSAW) was used for the detection of food pathogenic Escherichia coli O157:H7 (E.coli O157:H7), a dangerous strain among 225 E. coli unique serotypes. A few cells of this bacterium are able to cause young children to be most vulnerable to serious complications. Presence of higher than 1cfu E.coli O157:H7 in 25g of food has been considered as a dangerous level. The SHSAW biosensor was fabricated on 64° YX LiNbO3 substrate. Its sensitivity was enhanced by depositing 130.5nm thin layer of SiO2 nanostructures with particle size lesser than 70nm. The nanostructures act both as a waveguide as well as a physical surface modification of the sensor prior to biomolecular immobilization. A specific DNA sequence from E. coli O157:H7 having 22 mers as an amine-terminated probe ssDNA was immobilized on the thin film sensing area through chemical functionalization [(CHO-(CH2)3-CHO) and APTES; NH2-(CH2)3-Si(OC2H5)3]. The high-performance of sensor was shown with the specific oligonucleotide target and attained the sensitivity of 0.6439nM/0.1kHz and detection limit was down to 1.8femto-molar (1.8×10(-15)M). Further evidence was provided by specificity analysis using single mismatched and complementary oligonucleotide sequences.

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.