Affiliations 

  • 1 SRM Research Institute, SRM Institute of Science and Technology, Kattankulathur, Chennai, Tamil Nadu, 603 203, India
  • 2 Endocrine and Exposome Laboratory, Department of Zoology, Madras Christian College, Tambaram, Chennai, Tamil Nadu, 600 059, India
  • 3 Innovation and Technology Advancement Department, GK Aqua Sdn Bhd, Lot 563 Kg Sg Machang Ulu, 71750, Lenggeng, Negeri Sembilan, Malaysia
  • 4 Molecular Biophysics Lab, School of Chemical and Biotechnology, SASTRA Deemed to be University, Thanjavur, Tamil Nadu, 613 401, India
  • 5 Department of Zoology, Faculty of Science, King Saud University, Riyadh, 11451, Saudi Arabia
  • 6 Department of Zoology, Vaccines Research Unit, College of Science, King Saud University, Riyadh, 11451, Saudi Arabia
  • 7 Aquaculture Division, National Institute of Oceanography and Fisheries (NIOF), Alexandria, 21556, Egypt
  • 8 School of Processing Technology, Kerala University of Fisheries and Ocean Studies, Panangad, Kerala, India
  • 9 International Institute of Aquaculture and Aquatic Sciences (I-AQUAS), Universiti Putra Malaysia, 71050, Port Dickson, Negeri Sembilan, Malaysia
  • 10 SRM Research Institute, SRM Institute of Science and Technology, Kattankulathur, Chennai, Tamil Nadu, 603 203, India. jesuaraj@hotmail.com
Fish Physiol Biochem, 2021 Apr;47(2):293-311.
PMID: 33394283 DOI: 10.1007/s10695-020-00912-7

Abstract

This study reports the antioxidant property and molecular mechanism of a tryptophan-tagged peptide derived from a teleost fish Channa striatus of serine threonine-protein kinase (STPK). The peptide was tagged with tryptophan to enhance the antioxidant property of STPK and named as IW13. The antioxidant activity of IW13 peptide was investigated using in vitro methods such as DPPH, ABTS, superoxide anion radical scavenging and hydrogen peroxide scavenging assay. Furthermore, to investigate the toxicity and dose response of IW13 peptide on antioxidant defence in vitro, L6 myotubes were induced with generic oxidative stress due to exposure of hydrogen peroxide (H2O2). IW13 peptide exposure was found to be non-cytotoxic to L6 cells in the tested concentration (10, 20, 30, 40 and 50 μM). Also, the pre-treatment of IW13 peptide decreased the lipid peroxidation level and increased glutathione enzyme activity. IW13 peptide treatment upregulated the antioxidant enzyme genes: GPx (glutathione peroxidase), GST (glutathione S transferase) and GCS (glutamine cysteine synthase), in vitro in L6 myotubes and in vivo in zebrafish larvae against the H2O2-induced oxidative stress. The results demonstrated that IW13 renders protection against the H2O2-induced oxidative stress through a cellular antioxidant defence mechanism by upregulating the gene expression, thus enhancing the antioxidant activity in the cellular or organismal level. The findings exhibited that the tryptophan-tagged IW13 peptide from STPK of C. striatus could be a promising candidate for the treatment of oxidative stress-associated diseases.

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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